Search Strains

Chemosensory abnormal. Slightly small. Defective dauer formation. M-MATING NO SUCCESS.

Throws 11% males.

Mechanosensory abnormal. Neurons/Touch abnormal. Lethargic. M-MATING++ 1-10%WT.

Dauer defective. Non-crowder. M-MATING++++ >30%WT.

Dauer defective. Not leaky. Crowds. Amphid defect by EM. M-MATING-NO SUCCESS.

Dauer defective. Crowds. Chemotaxis defective-Na+. M-MATING-NO SUCCESS.

Suppressor weak. eDp22 = sup-3(e1390).

Vulvaless. Recessive. M-MATING+++ 10-30%WT.

Unc.

Dpy. Recessive. M-MATING+POOR <1%WT.

Dauer defective. Long.

tra-3 suppressed. Viable hermaphrodite. See also CGC 397.

Lineage abnormal. Recessive. Segregates males. Males abnormal.

ts

Maternal effect tra-3. Heterozygotes are WT and segregate WT and Dpy. Homozygous tra-3 animals are WT hermaphrodites which segregate abnormal sterile males or intersex.

Dpy. Recessive. M-MATING+POOR <1%WT.

Lineage abnormal. Bursae abnormal. Segregates abnormal males. M-MATING-NO SUCCESS.

Extra hypodermal nuclei. DTC migration abnormal. pka lin-20.

Heterozygotes are WT and segregate WT, Dpy, Vul, and dead eggs. The Dpys throw Dpy males. Maintain by picking WT.

WT phenotype. tra-3 amber mutant carrying homozygous linked amber suppressor.

Stable male/female strain propagated by crossing tra-3(e1767)IV females x tra-3(e1767)IV males. tra-1(e1575) is semi-dominant and transforms both XX and XO into fertile females. Strain contains 4 genotypes: 1) e1575/+; e1767 XX which are fertile females. 2) e1575/+; e1767 XO which are fertile females. 3) e1767 XX which are infertile pseudomales. 4) e1767 XO which are fertile males.

Heterozygotes are Unc (shaker; don't move well) and segregate more Shaker Unc, WT and DpyUnc (short and fairly paralyzed). The WT give only males. e1957 previously called sup-21.

Heterozygotes are WT and segregate WT, DpyUnc and fertile females.

Temperature sensitive Fem. Hermaphrodites at 15C, female at 25C. Maintain at 15C.

Hets are Unc and segregate Unc, WT hermaphrodites and dead eggs. The WT hermaphrodites segregate only abnormal sterile males or intersexes. Maintain by picking Unc.

Weakest known fem-3 mutation. Viable non-temperature-sensitive self-fertile XX hermaphrodites. e1999 XO males are slightly feminized, sometimes male-fertile. Reference: Hodgkin (1986) PMID: 3770465.

eIs2137 [sup-7(+) + Dm-hsp::lacZ] IV. lacZ expression induced by heat shock. Limited pattern (the pSHZ1 vector was originally designed for use in Drosophila, not C. elegans, and is not the best vector for expression of lacZ in the worm). This strain has predominantly historical value (as the first C. elegans strain shown to express a foreign gene). eIs2137 transgene is integrated and contains two plasmids: pAST (sup-7 amber suppressor), and pSHZ1 (lacZ driven by Drosophila heat shock promoter (S. Munro)).

Self-fertile intersexual XX animals. Tra-3 masculinized phenotype suppressed by egl-26 mutation. Reference: Hodgkin (1986) PMID: 3770465.

Viable Dpy at 15C. Extreme Dpy or L1 lethal at 25C.

Long, especially obvious in adults. Males mate well.

Hermaphrodites have an abnormal protrusive vulva. Segregate males with abnormally swollen bursa. Suppressor of certain alleles of certain genes.

Poorly growing, low self-fertility masculinized XX hermaphrodites. Weak allele of tra-2, partly suppressed to self-fertility by smg (NMD) mutation; permits efficient selection of new feminizing mutations. References: Spence et al. (1990) PMID: 2317869. Zarkower et al. (1994) PMID: 7520378.

ZZ21 lev-1(x21) tra-3(e2333) isolated by Jim Lewis as EMS-induced levamisole resistant derivative of N2. Found to carry a cryptic tra-3 allele by Jonathan Hodgkin. XX animals are viable hermaphrodites which produce 500 rather than 330 self-progeny; occasionally Egl; no other signs of masculinization.

Wild type hermaphrodite phenotype, since tra-3(e1107) is fully suppressed.

Paralyzed Unc. This strain carries a tightly linked mutation, che-3(ky1018), that changes Q1107 of che-3 to a stop codon. Reference: Larsch, et al. (2015) Cell Reports.

Wild-type hermaphrodites segregating wild-type hermaphrodites, Unc-24 females, Unc Dpy hermaphrodites. Maintain by picking wild-type hermaphrodites. Deletion allele of fem-1, with unusual maternal effect. Reference: Spence AM, et al. Cell. 1990 Mar 23;60(6):981-90. Johnson CL & Spence AM.. Science. 2011 Sep 2;333(6047):1311-4.

eEx24 [tra-3(+) + rol-6(su1006)]. Pick Rollers to maintain. Roller hermaphrodites producing more Rol hermaphrodites and non-Rol hermaphrodites that produce broods of 100% Tra-3 XX pseudomales. Tra-3 mutant rescued by transgenic tra-3(+); useful source of homozygous m+z- tra-3 XX hermaphrodites. Reference: Barnes & Hodgkin (1996) PMID: 8887539.

Small. Recessive. Males abnormal-abnormal spicules. M-MATING-NO SUCCESS.

Slow growth and movement. Egl. Somewhat Uncoordinated. Maternal effect: m+z- homozygotes have no obvious phenotype.

Him. Temperature-sensitive: infertile above 20C. Variably masculinized XX hermaphrodites segregating normal XO males. Stronger XX masculinization than null alleles of tra-3. Reference: Francis et al. (1995) PMID: 7713420.

Maintain by crossing males and hermaphrodites; may revert to purely hermaphrodite line. Fertile hermaphrodites and fertile males. Obligate XX strain with sex determined by her-1 V. Reference: Strain 9 in Hodgkin (2002) PMID: 12399387.

Resistant to infection by Microbacterium nematophilum (no tail swelling).

Male/hermaphrodite strain, propagate by crossing. Fertile XX hermaphrodites and fertile XX males. Obligate XX strain with sex determined by amber-suppressing tRNA, sup-34. Reference: Strain 14 in Hodgkin (2002) PMID: 12399387.

Dumpy (partially-suppressed Dpy-31). Sqt-3 mediated suppression of dpy-31 lethality. Reference: Novelli et al. (2004) PMID: 15579684.

Slightly dumpy. Variable cold-sensitive lethal (poor viability at 15C). Unusual missense allele (D297G) of sqt-3, suppresses dpy-31 lethality. Reference: Novelli et al. (2006) PMID: 16452136.

Homozygous viable dpy. dpy-31(e2919) homozygotes are almost inviable, but lethality is efficiently suppressed by sqt-3(e2906). Reference: Novelli J, et al., Genetics. 2004 Nov;168(3):1259-73.

Squat, dumpy at 15C. Inviable at higher temperatures. Deletion of most of sqt-3 coding sequence. Null by antibody staining.

Weak dumpy. Variable tail abnormal at 20C or higher. Cold-sensitive: most arrest as pretzel-stage embryos, with some escapers arresting as severely distorted L1 larvae at 15. Unusual missense allele (K280E) of sqt-3, cold-sensitive lethal and suppressor of dpy-31 lethality. Reference: Novelli et al. (2006) PMID: 16452136.

Notched head. Recessive. 100% Penetrance. Male spicules abnormal. M-MATING-NO SUCCESS. See also WBPaper00002236.

Surface abnormal. Resistant to infection by Microbacterium nematophilum (no tail swelling). Nonsense mutation Q89stop(UAA).