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Strain Species Genotype
CB4270 C. elegans tra-1(e2200) Show Description
Hermaphrodite at 15?, sterile and gonad-defective at 25?; feminized by smg (NMD) mutations.
CB4289 C. elegans tra-1(e1575e1816e1835) III; eDp6 (III;f). Show Description
Wildtype hermaphrodites segregating wildtype hermaphrodites and tra-1 XX males, which mate with greater efficiency than most tra-1 XX mutants. Reference: Hodgkin (1987) PMID:3428597.
DZ840 C. elegans tra-1(ez72[biotag::GFP::TEV::3xflag::tra-1]) III. Show Description
tra-1(ez72[biotag::GFP::TEV::3xflag::tra-1]) III.
CB2823 C. elegans tra-1(e1488) III; eDp6 (III;f). Show Description
Balanced well. Animals with the duplication are WT. Animals which have lost the duplication have a hermaphrodite gonad and intestine (they are self-fertile); the rest of the body is male.
CB2829 C. elegans tra-1(e1076) III; eDp6 (III;f). Show Description
Balanced well. Segregates males.
CB3631 C. elegans tra-1(e1929) III; eDp6 (III;f). Show Description
WT hermaphrodites which segregate WT hermaphrodites and tra-1 homozygous XX; weakly masculinized class A7, never fertile as males.
CB3989 C. elegans tra-1(q88) III; eDp6 (III;f). Show Description
Wildtype hermaphrodites segregating wild-type hermaphrodites and tra-1(q88) XX males, which are fertile and make yolk. Complex rearrangement of tra-1. Reference: Zarkower et al. (1994) PMID: 7520378
CB4900 C. elegans tra-1(rh132) III; eDp6 (III; f). Show Description
Fertile hermaphrodites segregating more fertile hermaphrodites and sterile Class A10 tra-1(rh132) XX homozygotes, which have hermaphrodite non-gonadal anatomy and male gonads; Smg (NMD)-sensitive. Reference: Zarkower et al. (1994) PMID: 7520378
CB5035 C. elegans tra-1(e1575 e1816 e1834) III; eDp25[tra-1(e1575 e1816)] (III; f). Show Description
Male/female strain, propagate by crossing. XX females and tra-1 XX males. Low fertility. Fertile females and fertile males; male-biased sex ratio. Strain with sex determined by a feminizing fragment chromosome (LGIIIR); unusual sex ratio. Reference: Strain 15 in Hodgkin (2002) PMID: 12399387.
DZ841 C. elegans tra-1(ez72[biotag::GFP::TEV::3xflag::tra-1]) III; zuIs236. Show Description
tra-1(ez72[biotag::GFP::TEV::3xflag::tra-1]) III. zuIs236 [his-72(1 kb 5'UTR)::BIRA::GFP::his-72(1 kb 3'UTR) + unc-119(+)]. Location of zuIs236 is not known, but is not in LG III.
BC12051 C. elegans dpy-5(e907) I; sEx12051. Show Description
sEx12051 [rCes Y47D3A.6b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525).
CB2590 C. elegans tra-1(e1099)/dpy-18(e1096) III. Show Description
Heterozygotes are wild-type, and segregate wild-type heterozygotes, fertile wild-type males (tra-1 homozygotes), and Dpy. Can recombine: pick individual WT hermaphrodites and check for correct segregation of progeny to maintain.
CB3769 C. elegans tra-1(e1575)/+ III; tra-3(e1767) IV. Show Description
Stable male/female strain propagated by crossing tra-3(e1767)IV females x tra-3(e1767)IV males. tra-1(e1575) is semi-dominant and transforms both XX and XO into fertile females. Strain contains 4 genotypes: 1) e1575/+; e1767 XX which are fertile females. 2) e1575/+; e1767 XO which are fertile females. 3) e1767 XX which are infertile pseudomales. 4) e1767 XO which are fertile males.
CB4559 C. elegans smg-2(e2008) I; tra-1(e1732) III. Show Description
Grows poorly, best at 20C. Self-fertile intersexual XX hermaphrodite due to partial suppression of weak tra-1 allele by smg-2(NMD). References: Zarkower et al. (1994) PMID: 7520378. Hodgkin (1987) PMID:3428597.
CB4613 C. elegans unc-32(e189) tra-1(e1575e1816e1831) III; eDp6 (III;f). Show Description
Unc-32 hermaphrodites segregating Unc hermaphrodites and Unc Tra homozygous XX pseudomales with variable male tails, hermaphroditic gonads and adult yolk production. Class B2 allele (male tail, sterile hermaphrodite gonad) derived by mutation of tra-(gf) allele e1575. Reference: Hodgkin (1987) PMID:3428597.
CB4890 C. elegans smg-2(e2008) I; tra-1(e1488) III. Show Description
Intersexual self-fertile XX hermaphrodites with Smg (protruding vulva) phenotype. Grows poorly, does best at 20C. Class B1 allele of tra-1 combined with smg-2(NMD), useful for selection of modifiers. Reference: Zarkower et al. (1994) PMID: 7520378
CB5133 C. elegans tra-1(e1732) III; eDp25[tra-1(e1575 e1816)] (III; f). Show Description
Male/female strain, propagated by crossing. eDp25 carries tra-1 gain-of-function allele. Anatomically normal XX females, tra-1(e1732) XO males, and intersexual tra-1(e1732) XX and e1732; eDp25 XO. References: Hodgkin (1987) PMID: 3428597. Zarkower et al. (1994) PMID: 7520378.
CB5200 C. elegans smg-2(e2008) I; tra-1(e2272) III. Show Description
Self-fertile hermaprodite or female. Protruding vulva. tra-1(e2272) XX homozygotes are sterile with abnormal gonads; e2272 mutation is partly suppressed by NMD (smg-2(e2008)), hence double mutants are fertile. Reference: Zarkower et al. (1994) PMID: 7520378.
CP38 C. briggsae Cbr-tra-1(nm2)/Cbr-let(nm28) III. Show Description
When singled, hermaphrodites should throw 2/3 hermaphrodites and 1/2 nm2 XX males. The lethal appears to balance the nm2 allele pretty well, but precise recombination mapping has not been performed. The XX males maintain their phenotypic resemblance to the unbalanced strain and are probably not fertile due to obvious gonadal deficiencies. This strain has been successfully grown at 15C and 20C. Both strains appear to have complete penetrance of the mutant phenotypes.
BW1145 C. elegans tra-1(e1099) dpy-18(e364) III; ctDp2 (III;f). Show Description
Animals which carry ctDp2 are WT. Animals which have lost ctDp2 are Dpy Males. Maintain by picking WT.
CB7171 C. elegans tra-1(e1099) subs-4(e3026) III; eDp6 (III;f). Show Description
Pick wild-type to maintain. Wild-type hermaphrodites segregate wild-type and dead masculinized embryos. e3026 is nonsense mutation in essential gene Y47D3B.1. Reference: O'Rourke et al (in preparation).
EM185 C. elegans tra-1(e1099) III; ram-5(bx30) X; eDp6 (III;f). Show Description
Animals with the duplication are WT. XX Animals which have lost the duplication are males (low fertility-gonad morphology variable). Rays abnormal. See also WBPaper0000498.
CB5190 C. elegans tra-2(e2020) II; tra-1(e1099)/+ III; xol-1(y9) X. Show Description
Heterozygous. Maintain by picking fertile hermaphrodites. Stable XX male/female strain (fertile XX females and low fertility XX males); males sire only female progeny. Reference: Strain 3 in Hodgkin (2002) PMID: 12399387.
JK5657 C. elegans tra-1(q183) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I; III). Show Description
Maintain at 15C. Heterozygotes are WT, GFP+ in the pharynx and segregate WT (GFP+ in the pharynx), dead eggs (hT2 homozygotes) and non-GFP tra-1(q183) homozygous females. Raise strain at 15C; at 20C many heterozygotes are also feminized. tra-1(q183) is temperature-sensitive for its XX, but not its XO mutant phenotype. At 25C, both q183/q183 and q183/+ are female. At 15C, q183/q183 is still always female, but q183/+ can be hermaphroditic. Maintain by picking fertile GFP worms and checking for correct segregation, since the hT2 balancer is lost at low frequencies.
CB2810 C. elegans tra-1(e1575)/+ III; unc-42(e270) him-5(e1490) dpy-21(e428) V. Show Description
Pick Unc hermaphrodite to maintain (these will be tra-1/+; unc-42 him-5 dpy-21 XO animals). Segregates Unc hermaphrodites (tra-1/+ III), Unc males (+/+ III), and DpyUnc hermaphrodites (+/+ III). dpy-21 not expressed in XO. dpy-21(e428) V; XX animals are weak Dpy. dpy-21(e428) V; XO animals are non-Dpy.
BW1084 C. elegans ncl-1(e1865) unc-36(e251) tra-1(e1099) dpy-18(e364) III; ctDp6 (III;f). Show Description
Animals which carry ctDp6 are WT. Animals which have lost ctDp6 are DpyUnc Males. Maintain by picking WT.
CB5213 C. elegans tra-2(e2272e2624) II. Show Description
Intragenic partial revertant of tra-1(e2272). Fertile hermaphrodites or females. Reference: Zarkower et al. (1994) PMID: 7520378.
JK3361 C. elegans qIs76 I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III) Show Description
qIs76 [tra-1::GFP::beta-GAL + rol-6(su1006)]. Rollers express TRA-1::GFP in Z1/Z4, intestine, etc. qIs76 homozygotes are lethal. Heterozygotes are Rol GFP+ (pharynx). Pick Rollers with GFP+ pharynx to maintain. Reference: Chang W., et al. Development. 2004 Mar;131(6):1425-36.
OH15862 C. elegans him-5 (e1490) V; otEx7353. Show Description
otEx7353 [UPN::tagRFP::TRA-1(active) + rol-6(su1006)]. Rollers. Pick Rollers to maintain. Nervous system-specific expression of a TRA-1 gain-of-function construct. UPN is a concatenated panneuronal promoter (containing promoter fragments from unc-11, rgef-1, ehs-1, and ric-19). TRA-1(active) is a shortened version of the TRA-1A cDNA (860aa) predicted to encode the proteolytically activated version of TRA-1A generated by C-terminal cleavage in hermaphrodites (Schvarzstein and Spence 2006).
RA7 C. elegans rdEx1. Show Description
rdEx1 [GFP::tra-1 + rol-6(su1006)]. Rollers. Array can cause sterility. Maintain by picking gravid Rollers. GFP::tra-1 fusion protein. Reference: Mathies LD, et al. Development. 2004 Sep;131(17):4333-43.
TY1807 C. elegans xol-1(y9) X. Show Description
Fully penetrant XO lethal. XX is WT. Enhancer of her-1(sd), tra-2(lf), tra-3(lf) XX masculinization phenotypes. Does not enhance tra-1(lf).
TY525 C. elegans him-8(e1489) IV; xol-1(y9) X. Show Description
Fully penetrant XO lethal. XX is WT. Enhancer of her-1(sd), tra-2(lf), tra-3(lf) XX masculinization phenotypes. Does not enhance tra-1(lf).