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Strain Species Genotype
AF1 C. elegans +/szT1 [lon-2(e678)] I; dpy-8(e1321) unc-3(e151)/szT1 X. Show Description
Heterozygotes are WT and segregate WT, DpyUnc, dead eggs and Lon males. Maintain by picking WT.
BW1118 C. elegans mel-25(ct60) unc-42(e270)/unc-23(e25) vab-8(e1017) V; lon-2(e678) X. Show Description
Maintain by picking Lon non-Unc. Throws Lon non-Unc, Lon Unc Vab (bent head, tails very thin), and Lon Unc Mel (kinker, temperature-sterile).
CB2198 C. elegans daf-3(e1376) lon-2(e678) X. Show Description
Dauer defective. Long.
CB2199 C. elegans lon-2(e678) daf-6(e1377) X. Show Description
Dauer defective. Long.
CB2217 C. elegans lon-2(e678) mec-7(e1343) X. Show Description
Long. Mechanosensory abnormal. Semidominant Mec.
CB2238 C. elegans lon-2(e678) unc-84(e1410) X. Show Description
Lon. Unc.
CB2620 C. elegans daf-9(e1406)/lon-2(e678) X. Show Description
Heterozygotes are WT and segregate WT, lethal dauers (DAUER-LIKE LARVAE) and Lon.
CB3253 C. elegans dpy-23(e840) lon-2(e678) X. Show Description
Variably Dpy, slow growing. Inviable on MYOB medium.
CB3270 C. elegans lon-2(e678) mec-7(e1506) X. Show Description
Long. Mechanosensory abnormal. Severe mechanosensory abnormal. Recessive.
CB3271 C. elegans lon-2(e678) mec-10(e1515) X. Show Description
Long. Mechanosensory abnormal.
CB3273 C. elegans lon-2(e678) mec-2(e75) X. Show Description
Long. Mechanosensory Abnormal. [NOTE: originally described as carrying e1084, this strain actually carries the e75 allele. The lesion has been verified by sequence analysis. (11/26/2018)]
CB3274 C. elegans lon-2(e678) mec-4(e1497) X. Show Description
Long. Mechanosensory abnormal.
CB3275 C. elegans lon-2(e678) mec-5(e1504) X. Show Description
Long. Mechanosensory abnormal.
CB3276 C. elegans lon-2(e678) mec-7(e1527) X. Show Description
Dominant Mechanosensory Abnormal at 20C. Long.
CB3475 C. elegans mcm-4(e1466)/szT1 [lon-2(e678)] I; +/szT1 X. Show Description
Heterozygotes are WT and segregate WT, Lon males and thin sterile Unc animals. Maintain by picking WT.
CB3533 C. elegans +/szT1 [lon-2(e678)] I; twk-18(e1913)/szT1 X. Show Description
Heterozygotes are Unc and segregate Unc, dead eggs, and Lon males. e1913 is a dominant Unc and recessive lethal. Maintain by picking Unc. e1913 previously called unc-110.
CB5042 C. elegans lon-2(e678)^lon-2(e678) X. Show Description
Attached X chromosome line. Long hermaphrodites with low fertility, producing some X^X X^X dead eggs. May spontaneously revert to unattached lon-2 XX. Reference: Hodgkin & Albertson (1995) PMID: 8647390.
CB5310 C. elegans unc-4(e120) II; lon-2(e678)^lon-2(e678) X. Show Description
Attached X strain with autosomal unc-4 marker, which facilitates test-crosses. Uncoordinated Long hermaphrodites, reduced fertility with many X^X X^X unhatched eggs. Reference: Hodgkin & Albertson (1995) PMID: 8647390.
CB678 C. elegans lon-2(e678) X. Show Description
Long. Mapping marker standard. M-MATING++++ >30%WT.
CB7538 C. elegans lon-2(e678) bus-8(ok1176) X. Show Description
Very slow-growing, grows best at 25C. Small, bleach-sensitive, Bus, resistant to Leucobacter Verde1. Reference: O'Rourke et al (in preparation).
CGC28 C. elegans +/szT1 [lon-2(e678) umnIs17] I; dpy-8(e1321) unc-3(e151)/szT1 X. Show Description
umnIs17 [myo-2p::GFP + NeoR, X: 6745526 (intergenic)] I. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, DpyUnc non-GFP, dead eggs and GFP+ Lon males. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into szT1 balancer in parental strain AF1 using CRISPR/Cas9.
CGC35 C. elegans +/szT1 [lon-2(e678) umnIs24] I; dpy-8(e1321) unc-3(e151)/szT1 X. Show Description
umnIs24 [myo-2p::GFP + NeoR, X: 15420938 (intergenic)] I. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, DpyUnc non-GFP, dead eggs and GFP+ Lon males. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into szT1 balancer in parental strain AF1 using CRISPR/Cas9.
CGC40 C. elegans +/szT1 [lon-2(e678) umnIs29] I; dpy-8(e1321) unc-3(e151)/szT1 X. Show Description
umnIs29 [myo-2p::mKate2 + NeoR, X: 6745526 (intergenic)] I. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc non-GFP, dead eggs and mKate2+ Lon males. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate2 transgene into szT1 balancer in parental strain AF1 using CRISPR/Cas9.
CGC41 C. elegans +/szT1 [lon-2(e678) umnIs30 umnIs24] I; dpy-8(e1321) unc-3(e151)/szT1 X. Show Description
umnIs30 [myo-2p::mKate2 + NeoR, X: 6745526 (intergenic)] I. umnIs24 [myo-2p::GFP + NeoR, X: 15420938 (intergenic)] I. Carries szT1 balancer with mKate2 tag on left arm and GFP tag on right arm. Heterozygotes are wild-type GFP+ & mKate2+, and segregate wild-type GFP+ & mKate2+, DpyUnc non-GFP & non-mKate2, dead eggs and GFP+ & mKate2+ Lon males. Maintain by picking wild-type with GFP+ & mKate2+. Derived by insertion of myo-2p::mKate transgene into szT1 balancer in parental strain CGC35 using CRISPR/Cas9.
CGC46 C. elegans +/szT1 [lon-2(e678)] I; dpy-8(e1321) unc-3(e151)/szT1 [umnIs35] X. Show Description
umnIs35 [myo-2p::GFP + NeoR, I: 6284001 (intergenic)] X. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, DpyUnc non-GFP, dead eggs and GFP+ Lon males. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into szT1 balancer in parental strain AF1 using CRISPR/Cas9.
CGC50 C. elegans +/szT1 [lon-2(e678) umnIs39] I; dpy-8(e1321) unc-3(e151)/szT1 [umnIs40] X. Show Description
umnIs39 [myo-2p::mKate2 + NeoR, X: 6745526 (intergenic)] I. umnIs40 [myo-2p::GFP + NeoR, I: 6284001 (intergenic)] X. Heterozygotes are wild-type GFP+ & mKate2+, and segregate wild-type GFP+ & mKate2+, DpyUnc non-GFP & non-mKate2, dead eggs, and GFP+ & mKate2+ Lon males. Maintain by picking wild-type with GFP+ & mKate2+. Derived by simultaneous insertion of independent myo-2p::mKate2 and myo-2p::GFP transgenes into each portion of the szT1 balancer in parental strain AF1 using CRISPR/Cas9.
CGC79 C. elegans +/szT1 [lon-2(e678) umnIs61] I; dpy-8(e1321) unc-3(e151)/szT1 X. Show Description
umnIs61 [myo-2p::mKate2 + NeoR, X: 15420938 (intergenic)] I. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc non-mKate2, dead eggs and mKate2+ Lon males. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate2 transgene into szT1 balancer in parental strain AF1 using CRISPR/Cas9.
CGC97 C. elegans +/szT1 [lon-2(e678)] I; dpy-8(e1321) unc-3(e151)/szT1 [umnIs77] X. Show Description
umnIs77 [myo-2p::mKate2 + NeoR, I: 6284001 (intergenic)] X. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc non-mKate2, dead eggs and mKate2+ Lon males. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate2 transgene into szT1 balancer in parental strain AF1 using CRISPR/Cas9.
CS624 C. elegans daf-2(e1370) III; lon-2(e678) X. Show Description
Temperature-sensitive. Maintain at 15C. Lon. Daf-c.
DA1077 C. elegans egl-30(ad810) dpy-5(e61)/szT1 [lon-2(e678)] I; +/szT1 X. Show Description
ad810 is homozygous lethal. ad810/+ is Egl and it suppresses gpb-2 (a.k.a. eat-11). In DA1077, heterozygotes are Egl. Strain throws Lon Males.
DA1096 C. elegans egl-30(ad810)/szT1 [lon-2(e678)] I; +/szT1 X. Show Description
ad810 is homozygous lethal. ad810/+ is Egl and it suppresses gpb-2 (a.k.a. eat-11). In DA1096, heterozygotes are Egl. Throws Lon males.
DA438 C. elegans bli-4(e937) I; rol-6(e187) II; daf-2(e1368) vab-7(e1562) III; unc-31(e928) IV; dpy-11(e224) V; lon-2(e678) X. Show Description
Linkage mapping strain. Maintain at 15C.
DA658 C. elegans lon-2(e678) npr-1(n1353) X. Show Description
Long. Clumps. npr-1 pka bor-1.
DA742 C. elegans lon-2(e678) unc-6(e78) egl-15(n484) X. Show Description
Long. Uncoordinated. Egl.
DR112 C. elegans lon-2(e678) him-4(e1267) X. Show Description
Long. Segregates males.
DR113 C. elegans lon-2(e678) mec-4(e1339) X. Show Description
Long. Mechanosensory Abnormal. Sometimes touch sensitive in tail.
DR205 C. elegans lon-2(e678) daf-12(m20) X. Show Description
Dauer defective. Long.
DR733 C. elegans daf-9(e1406) lon-2(e678)/unc-78(e1217) X. Show Description
Balanced lethal dauer constitutive. Heterozygotes are WT and segregate WT, LonDaf and Unc. Will get 3-5 adult Lon per clone. Maintain by picking WT.
DR921 C. elegans dpy-7(sc27) daf-9(e1406)/lon-2(e678) X. Show Description
Heterozygotes are WT. At 25C the hets segregate DpyDafs, WT and Lon. At 15C the hets segregate Dafs, WT and Lon. [dpy-7(sc27) is temperature sensitive. At 25C it is larger than most Dpys and has a tendency to roll. Not expressed at 15C.] Maintain by picking WT.
EE31 C. elegans mup-2(e2346) unc-6(e78)/lon-2(e678) X. Show Description
Heterozygotes are WT and segregate WT, Lon and Uncs. If raised at 25C, the Uncs are Mup -> 3-fold/L1 arrest. If raised at 15C the Uncs are fertile (reduced brood).
EG1020 C. elegans bli-6(sc16) IV; dpy-11(e224) V; lon-2(e678) X. Show Description
Dpy suppresses Bli and Lon. Strain appears to be only slightly Dpy. Useful for mapping, especially Unc mutations. Separately, dpy-11 causes dumpiness, bli-6 adult worms develop blisters on their bodies, and lon-2 worms are about 150% WT length.
EU307 C. elegans +/szT1 [lon-2(e678)] I; mom-1(or46) dpy-6(e14)/szT1 X. Show Description
Heterozygotes are WT and segregate WT, Lon males, lots of dead eggs, and Dpys. The Dpys should give all dead embryos at all temperatures. Approximately 60% of these dead embryos make excess mesoderm at the expense of endoderm, due to an E to MS fate transformation. Embryonic lethality is 100% penetrant for or46.
EU308 C. elegans +/szT1 [lon-2(e678)] I; mom-1(or10) dpy-6(e14)/szT1 X. Show Description
Heterozygotes are WT and segregate WT, Lon males, lots of dead eggs, and Dpys. The Dpys should give all dead embryos at all temperatures. Approximately 60% of these dead embryos make excess mesoderm at the expense of endoderm, due to an E to MS fate transformation. Embryonic lethality is 100% penetrant for or10.
EU361 C. elegans +/szT1 [lon-2(e678)] I; mom-1(or10) unc-6(n102)/szT1 X. Show Description
Heterozygotes are WT and segregate WT, Uncs with a protruding vulva, Long males and dead eggs. Uncs give only embryonic lethals which lack gut and make excess mesoderm (E adopts an MS-like fate). or10 is a recessive maternal-effect embryonic lethal. Zygotic phenotype of or10 is protruding vulva and slight uncoordination.
FX11552 C. elegans vps-45(tm246)/egl-17(e1313) lon-2(e678) X. Show Description
Heterozygotes are WTand segregate WT, Egl Lon, and tm246 homozygotes (temperature sensitive lethal which can be maintained as homozygotes at 15C). tm246 homozygotes have endocytosis defects in oocytes and coelomocytes at 25C. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
GE1076 C. elegans pha-1(e2123) III; lon-2(e678) X. Show Description
Long. Maintain at 15C. pha-1(e2123) is a temperature sensitive embryonic lethal. Strain viable only at 15C. lon-2 included to facilitate microinjection. Check regularly for ts lethality-spontaneous sup-35 mutations may occur!
GE1386 C. elegans tDf3 dpy-5(e61)/szT1 [lon-2(e678)] I; dpy-8(sc44)/szT1 X. Show Description
Heterozygotes are WT and segregate WT, Lon males and dead eggs. Strain will occasionally throw some DpysRollers.
GE1549 C. elegans tDf4 dpy-5(e61)/szT1 [lon-2(e678)] I; dpy-8(sc44)/szT1 X. Show Description
Heterozygotes are WT and segregate WT, dead eggs, and Lon males. Maintain by picking WT. Strain will occasionally throw some DpysRollers.
GR1028 C. elegans mnDp33 (X;IV)/+ IV; let-43(mg49) lon-2(e678) X. Show Description
Animals heterozygous for mnDp33 are Lon and segregate Lon and larval lethals. (Animals which have lost mnDp33 are larval lethal. Animals which are homozygous for mnDp33 are also larval lethal (L1-L2).)
GR1029 C. elegans let-44(mg41) lon-2(e678) X; mnDp31 (X;f). Show Description
Animals with mnDp31 are WT and segregate WT and dead embryos. (Animals which have lost mnDp31 arrest as embryos.)