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Strain Species Genotype
RW12259 C. elegans unc-3(st12259[unc-3::TY1::EGFP::3xFLAG]) II. Show Description
CRISPR/Cas9 engineered tagged endogneous locus.
CB151 C. elegans unc-3(e151) X. Show Description
Unc.
KRA437 C. elegans unc-3(n3435) X; kasEx147. Show Description
kasEx147 [oig-1p(1.6kb)::GFP::unc-54 3'UTR + myo-2p::GFP]. Pick worms with GFP+ pharynx to maintain array. Unc. 1.6kb cis-regulatory region (-2.6-1.0kb) upstream of oig-1 was fused to GFP with the unc-54 3'UTR. oig-1 uses mulitple cis-regulatory regions to achieve different expression patterns in motor neurons; this construct drives expression specifically in cholinergic motor neurons. Construct was injected into N2 worms. Reference: Feng W, et al. Elife. 2020 Jan 3;9. pii: e50065. doi: 10.7554/eLife.50065.
KRA439 C. elegans unc-3(n3435) X; kasEx149. Show Description
kasEx149 [oig-1p(2.6kb_del)::GFP::unc-54 3'UTR + myo-2p::GFP]. Pick worms with GFP+ pharynx to maintain array. Unc. 2.6kb cis-regulatory region with 200bp deletion removing predicted LIN-39 binding site upstream of oig-1 was fused to GFP. Expression of oig-1::GFP in GABAergic motor neurons is been abolished; expression was observed specifically in cholinergic motor neurons of the ventral nerve cord. Reference: Feng W, et al. Elife. 2020 Jan 3;9. pii: e50065. doi: 10.7554/eLife.50065.
KRA441 C. elegans unc-3(n3435) X; kasEx151. Show Description
kasEx151 [oig-1p(2.6kb_del)::GFP::unc-54 3'UTR + myo-2p::GFP]. Pick worms with GFP+ pharynx to maintain array. Unc. 2.6kb cis-regulatory region with 300bp deletion removing predicted LIN-39 binding site upstream of oig-1 was fused to GFP. Expression of oig-1::GFP in GABAergic motor neurons is retained, but eliminates ectopic expression in cholinergic motor neurons of the ventral nerve cord. Reference: Feng W, et al. Elife. 2020 Jan 3;9. pii: e50065. doi: 10.7554/eLife.50065.
MT10362 C. elegans unc-3(n3366) X. Show Description
MT10480 C. elegans unc-3(n3413) X. Show Description
OH13988 C. elegans ieSi57 II; unc-3(ot837[unc-3::mNeonGreen::AID]) X. Show Description
ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. The endogenous unc-3 locus is tagged with mNeonGreen and AID degron. mNeonGreen expression is seen in the cholinergic motor neurons, command interneurons, and ASI. Reference: Patel T & Hobert O. eLife 2017.
DL7 C. elegans wdIs4 II; unc-3(n3435) X. Show Description
wdIs4 [unc-4p::GFP + dpy-20(+)] II.
OH10850 C. elegans unc-3(e151) X; otEx4432. Show Description
otEx4432 [ace-2p(SL2 fosmid)::GFP + elt-2::DsRed2]. Fosmid-based reporter. Maintain by picking DsRed2(+) animals. Reference: Kratsios P, et al. Nat Neurosci. 2011 Nov 27. doi: 10.1038/nn.2989.
SP115 C. elegans mnDp8 (X;I); unc-3(e151) X. Show Description
WT strain.
SP116 C. elegans mnDp9 (X;I); unc-3(e151) X. Show Description
WT strain.
SP117 C. elegans mnDp10 (X;I); unc-3(e151) X. Show Description
WT strain. Occassionally Uncs appear.
SP122 C. elegans unc-3(e151) X; mnDp2 (X;f). Show Description
Strain throws WT and Uncs. Maintain by picking WT.
SP123 C. elegans unc-3(e151) X; mnDp3 (X;f). Show Description
Strain throws WT and Uncs. Maintain by picking WT.
SP124 C. elegans unc-3(e151) X; mnDp4 (X;f). Show Description
Strain throws WT and Uncs. Maintain by picking WT.
SP125 C. elegans unc-3(e151) X; mnDp5 (X;f). Show Description
FREE DUPLICATION. Strain throws WT and Uncs. Maintain by picking WT.
SP219 C. elegans mnDp1 (X;V)/+ V; unc-3(e151) X. Show Description
Pick WT to maintain. Segregates lethals. Dp/Dp are homozygous lethal.
SP517 C. elegans mnT9 (I;X)/unc-3(e151) X. Show Description
SP748 C. elegans unc-3(e151) X; mnDp12( X;f). Show Description
Strain throws WT and Uncs. Maintain by picking WT.
SP75 C. elegans mnDp25 (X;I)/+ I; unc-3(e151) X. Show Description
Strain throws WT and Unc. Maintain by picking WT.
SP76 C. elegans mnDp27 (X;II) II; unc-3(e151) X. Show Description
WT phenotype.
SP816 C. elegans unc-3(e151) X; mnDp14 (X;f). Show Description
Strain throws WT and Unc. Maintain by picking WT.
CGC36 C. elegans mnDp1 [umnIs25] (X;V)/+ V; unc-3(e151) X. Show Description
umnIs25 [myo-2p::GFP + NeoR, X: 15420938 (intergenic)]. Pick wild-type GFP+ to maintain. Segregates lethals: homozygous Dp/Dp are lethal. Derived by insertion of myo-2p::GFP transgene into mnDp1 duplication in parental strain SP219 using CRISPR/Cas9.
CGC37 C. elegans unc-3(e151) X; mnDp3 [umnIs26] (X;f). Show Description
umnIs26 [myo-2p::GFP + NeoR, X: 15420938 (intergenic)]. Pick wild-type GFP+ to maintain. Segregates wild-type GFP+ and Unc GFP-. Derived by insertion of myo-2p::GFP transgene into mnDp3 duplication in parental strain SP123 using CRISPR/Cas9.
CGC42 C. elegans mnDp10 [umnIs31] (X;I); unc-3(e151) X. Show Description
umnIs31 [myo-2p::GFP + NeoR, X: 15420938 (intergenic)] I. Segregates mostly wild-type GFP+ and occasional Unc GFP-. Maintain by picking WT GFP+. Derived by insertion of myo-2p::GFP transgene into mnDp10 duplication in parental strain SP117 using CRISPR/Cas9.
DR111 C. elegans him-4(e1266) unc-3(e151) X. Show Description
Unc. Segregates males.
DR1290 C. elegans dpy-6(e14) unc-3(e151) X. Show Description
Medium sized Dpy which curls its tail.
KRA102 C. elegans lin-39(kas1) III; ynIs40 V; unc-3(n3435) X. Show Description
ynIs40 [flp-11p::GFP] V. kas1 is a point mutation in the second intron splice acceptor site, disrupting splicing. Worms are vulvaless and have severe locomotion defects. Reference: Feng W, et al. Elife. 2020 Jan 3;9. pii: e50065. doi: 10.7554/eLife.50065.
OH10819 C. elegans unc-3(e151) X; vsIs48; otEx4441. Show Description
otEx4441 [hsp-16.2p::unc-3(cDNA) + ttx-3::mCherry]. vsIs48 [unc-17::GFP]. GFP expressed in all cholinergic neurons. Maintain by picking mCherry(+) animals. Reference: Kratsios P, et al. Nat Neurosci. 2011 Nov 27. doi: 10.1038/nn.2989.
OH10851 C. elegans juIs14 IV; unc-3(e151) X; otEx4812. Show Description
otEx4812 [unc-30p(2.6kb promoter)::unc-3(cDNA)::unc-54 3'UTR + myo-2::GFP]. juIs14 [acr-2p::GFP + lin-15(+)]. Maintain by picking myo-2::GFP(+) animals. Reference: Kratsios P, et al. Nat Neurosci. 2011 Nov 27. doi: 10.1038/nn.2989.
PR946 C. elegans unc-3(p1001) ace-1(p1000) X. Show Description
Acetylcholine abnormal. Kinky Unc.
SP14 C. elegans unc-3(e151) unc-7(e139) X. Show Description
SP15 C. elegans unc-9(e101) unc-3(e151) X. Show Description
SP394 C. elegans mnDp1(X;V)/+ V; unc-3(e151) mnDf41 X. Show Description
WT PHENOTYPE.
AV311 C. elegans dpy-18(e364) unc-3(e151) meT7 (III;X;IV). Show Description
Dpy. Unc. meT7 is an end-to-end-to-end fusion of chromosomes III, X, and V. The right end of III is fused to the left end of X, and the right end of X is fused to the left end of IV. Constructed by crossing eT5 and mnT12. meT7 homozygotes produce 92% viable progeny. meT7 heterozygotes are Him and produce many dead eggs.
MT2490 C. elegans unc-3(e151) lin-15B&lin-15A(n765) X. Show Description
Unc. Temperature sensitive Muv.
MT2718 C. elegans let-2(n1279) unc-3(e151) X; mnDp26 (X;f). Show Description
n1279 is lethal. Animals which are alive contain the duplication. n1279 pka sup-20.
PS1478 C. elegans unc-3(e151) lin-15B&lin-15A(e1763) X. Show Description
Unc. Muv. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
RW1385 C. elegans mnDp1 (X;V)/+ V; unc-3(e151) pat-9(st558) X. Show Description
Animals with the duplication are WT and segregate WT, sterile and Pats. Strain can be propagated by chunking.
SP223 C. elegans mnDp1 (X;V)/+ V; unc-3(e151) let-3(mn104) X. Show Description
LETHAL EARLY LARVAL Pick WT to maintain.
SP224 C. elegans mnDp1 (X;V)/+ V; let-4(mn105) unc-3(e151) X. Show Description
Lethal early larval. Maintain by picking WT.
SP225 C. elegans mnDp1 (X;V)/+ V; let-5(mn106) unc-3(e151) X. Show Description
LETHAL LATE LARVAL MATERNAL EFFECT Pick WT to maintain.
SP226 C. elegans mnDp1 (X;V)/+ V; let-9(mn107) unc-3(e151) X. Show Description
Pick WT to maintain. Throws WT, Sterile Uncs, and dead eggs(homozygous Dp).
SP231 C. elegans mnDp1(X;V)/+ V; unc-3(e151) let-7(mn112) X. Show Description
LETHAL LATE LARVAL SEX-INFLUENCED Maintain by picking WT.
SP232 C. elegans mnDp1 (X;V)/+ V; unc-3(e151) let-10(mn113) X. Show Description
LETHAL EARLY LARVAL. Maintain by picking WT.
SP233 C. elegans mnDp1(X;V)/+ V; unc-3(e151) let-2(mn114) X. Show Description
SP235 C. elegans mnDp1 (X;V)/+ V; unc-3(e151) let-11(mn116) X. Show Description
LETHAL EARLY LARVAL. Maintain by picking WT.
SP236 C. elegans mnDp1 (X;V)/+ V; unc-3(e151) let-16(mn117) X. Show Description
FERTILITY POOR LETHAL EARLY LARVAL Maintain by picking WT.
SP238 C. elegans mnDp1 (X;V)/+ V; unc-3(e151) let-1(mn119) X. Show Description
LETHAL EARLY LARVAL. Maintain by picking WT.