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Strain Species Genotype
CB1600 C. elegans tra-2(f70) II. Show Description
Transforms XX to males. Variable expression. Masculinized self-fertile hermaphrodite. tra-2(f70) was crossed into the N2 background to construct a dpy-10 tra-2 unc-4 triple mutant, then crossed to remove the flanking dpy and unc (Jonathan Hodgkin).
CB3778 C. elegans tra-2(e2020) II. Show Description
Obligate XX female, XO male strain. Maintain by mating. e2020 is a dominant mutation transforming XX into fertile females. XO animals are partly feminized by starvation or elevated temperatures, therefore the strain cannot be grown at 25C.
CB3779 C. elegans tra-2(e2021) II. Show Description
tra-2 gain-of-function. Male-female strain. Maintain by mating. References: EMBO J. 2001 Mar 15; 20(6): 1363–1372. Proc Natl Acad Sci U S A. 2004 Aug 24; 101(34): 12549–12554.
CB3967 C. elegans tra-2(e2046) II. Show Description
Obligate XX female, XO male strain. Maintain by mating. Viable 15-25C. e2046 is a dominant mutation transforming XX into fertile female; minimal effect on XO.
CB4905 C. elegans tra-2(e1403) II. Show Description
Weak Tra-2 allele, homozygous self-fertile XX. Slightly masculinized hermaphrodite, adults lack tail spike. Reference: Hodgkin & Barnes (1991) PMID: 1684664.
CB4952 C. elegans tra-2(e2541) II. Show Description
Hypomorphic allele. Homozygous XX animals are masculinized; have hermaphrodite gonad, abnormal male non-gonadal anatomy; self-fertile.
DH202 C. elegans tra-2(b202) II. Show Description
Temperature sensitive. XX animals are self-fertile hermaphrodites at 15C. XX animals are sterile males at 25C. ts period begins in late embryo.
JK726 C. elegans tra-2(q122) II. Show Description
tra-2(q122) is a gain-of-function allele. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
MT2292 C. elegans tra-2(n1106) II. Show Description
HSN(-) Egl. Weak allele.
PK125 C. elegans tra-2(e2531) II. Show Description
tra-2(e2531eg) Enhanced gain-of-function. Dominant allele that transforms XO animals into hermaphrodites. PK125 is composed of phenotypically WT hermaphrodites.
RB1949 C. elegans tra-2(ok2563) II. Show Description
C15F1.3 Homozygous. Outer Left Sequence: aaccagaaaagtcgccttga. Outer Right Sequence: tccacatcaagcatccagaa. Inner Left Sequence: ttggtgtgatggcaaagatg. Inner Right Sequence: atgcattcctgcgattcttc. Inner Primer PCR Length: 3370. Deletion size: about 1300 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
CB5213 C. elegans tra-2(e2272e2624) II. Show Description
Intragenic partial revertant of tra-1(e2272). Fertile hermaphrodites or females. Reference: Zarkower et al. (1994) PMID: 7520378.
CB2317 C. elegans tra-2(e1425) II; sup-5(e1464) III. Show Description
tra-2 suppressed. Viable hermaphrodite. See also CGC 397. Grow between 22.5C and 24C.
CB2580 C. elegans tra-2(e1094)/dpy-10(e128) II. Show Description
Heterozygotes are WT and segregate WT, Dpy and males.
CB4389 C. elegans tra-2(e1209) II; smg-3(ma117) IV. Show Description
Poorly growing, low self-fertility masculinized XX hermaphrodites. Weak allele of tra-2, partly suppressed to self-fertility by smg (NMD) mutation; permits efficient selection of new feminizing mutations. References: Spence et al. (1990) PMID: 2317869. Zarkower et al. (1994) PMID: 7520378.
CB5362 C. elegans tra-2(ar221) II; xol-1(y9) X. Show Description
ar221 is a tra-2 ts allele. Strain will grow at 15C as Egl hermaphrodites. When grown at 25C, all animals mature into X males, many of which are fertile. Good source of pure XX mating males. ar221 isolated by Jane Hubbard.
DZ683 C. elegans tra-2(ar221) II; xol-1(y9) X; rdIs4. Show Description
rdIs4 [ehn-3a::Venus(delta)]. Temperature-sensitive. Must be maintained at 15°C to produce progeny. Strain develops as hermaphrodites at 15°C (some animals are intersex with male tails), and develops as XX pseudomales at 25°C. GFP expressed in gonadal precursors. Reference: Kroetz MB & Zarkower D. G3 (Bethesda). 2015 Oct 23;5(12):2831-41.
CB2597 C. elegans tra-2(e1098)/dpy-10(e128) unc-4(e120) II. Show Description
Heterozygotes are WT and segregate WT, DpyUnc and males. Maintain by picking WT hermaphrodites.
CB2754 C. elegans tra-2(e1095)/dpy-10(e128) unc-4(e120) II. Show Description
Heterozygotes are WT and segregate WT, DpyUnc and males. Maintain by picking WT.
CB2830 C. elegans tra-2(e1209)/dpy-10(e128) unc-4(e120) II. Show Description
Balances well. Transformer weak. Heterozygotes are WT and segregate WT, DpyUnc and males. Maintain by picking WT.
CB4628 C. elegans tra-2(e1095) II; fem-1(e1927) IV; xol-1(y9) X. [XX females and tra-2; fem-1/+; xol-1 XX males] Show Description
Obligate male/female strain; maintain by crossing. Anatomically normal XX females and XX males. Male/female strain with sex determined by fem-1(+). Reference: Strain 11 in Hodgkin (2002) PMID: 12399387.
CB4706 C. elegans tra-2(e1095) II; fem-1(e1927) IV; xol-1(y9) X. [XX females and tra-2; fem-1; xol-1; eEx14[fem-1(+)] XX males] Show Description
Obligate male/female strain; maintain by crossing. Anatomically normal XX females and XX males. Male/female strain with sex determined by an extrachromosomal transgene array carrying fem-1(+). Reference: Strain 16 in Hodgkin (2002) PMID: 12399387.
CB4734 C. elegans tra-2(e1095)/+ II; fog-2(q71) V; xol-1(y9) X. [XX females and tra-2; fog-2; xol-1 XX males] Show Description
Obligate male/female strain; maintain by crossing. Anatomically normal XX females and XX males. Male/female XX strain with sex determined by tra-2(+). Reference: Strain 6 in Hodgkin (2002) PMID: 12399387.
CB5190 C. elegans tra-2(e2020) II; tra-1(e1099)/+ III; xol-1(y9) X. Show Description
Heterozygous. Maintain by picking fertile hermaphrodites. Stable XX male/female strain (fertile XX females and low fertility XX males); males sire only female progeny. Reference: Strain 3 in Hodgkin (2002) PMID: 12399387.
CB4050 C. elegans tra-2(e2046e2115) unc-4(e120) II; sup-7(st5) X. Show Description
Phenotypically wildtype XX hermaphrodite at 20C; poor viability and fertility at 25C. Rare XO animals are hermaphrodite due to suppression of amber mutation e2115 and consequent transformation of XO due to tra-2(e2046) gain-of-function. Reference: Hodgkin & Albertson (1995) PMID: 8647390.
CB5023 C. elegans tra-2(e2046e253) unc-4(e120) / + + II; dpy-26(n199) IV. [XO females, dpy-26 XO males] Show Description
Obligate XO male/female strain, propagate by crossing. Low fertility. Fertile XO females and XO males; inviable XX embryos and larvae. Reference: Strain 7 Hodgkin (2002) PMID: 12399387.
JK987 C. elegans tra-2(q276)/mnC1 [dpy-10(e128) unc-52(e444)] II. Show Description
Heterozygotes are WT and segregate WT, DpyUnc and males. The males will mate. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
ML503 C. elegans tra-2(q276)/rol-6(e187) II; mcDf2/dpy-8(e130) X. Show Description
Segregates WT, males, Dpy, Rol, DpyRol, dead eggs (mcDf2). Use XX tra-2 males to move mcDf2 around. mcDf2 is an approx. 500 kb deficiency removing a large chunk between mec-2 and sup-7 (non-inclusive).
CB4043 C. elegans smg-2(e2008) I; him-5(e1490) V. Show Description
e2008 recessively suppresses the phenotypes of mutations in unc-54(r293), lin-29(n546), tra-2 and dpy-5. Abnormal male tail. smg-2 homozygotes have slightly abnormal movement.
CP4 C. briggsae Cbr-dpy-?(nm4) II. Show Description
Dpy. Tightly linked to Cbr-tra-2 on LG II. Molecular identity unknown. Reference: Kelleher DF, et al. Genetics. 2008 Mar;178(3):1415-29.
CU7905 C. elegans smIs350 IV; unc-76(e911) V. Show Description
smIs350 [hsp-16::mCherry-NLS + tra-2::FLAG(3x) + unc-76(+)] IV. Some sterility. Maintain under normal conditions. Reference: Mapes J, et al. (2010) PNAS In press.
CU9087 C. elegans unc-76(e911) V; smIs380. Show Description
smIs380 [tra-2::GFP + unc-76(+)]. Some sterility. Maintain under normal conditions. Reference: Mapes J, et al. (2010) PNAS In press.
TY1807 C. elegans xol-1(y9) X. Show Description
Fully penetrant XO lethal. XX is WT. Enhancer of her-1(sd), tra-2(lf), tra-3(lf) XX masculinization phenotypes. Does not enhance tra-1(lf).
TY525 C. elegans him-8(e1489) IV; xol-1(y9) X. Show Description
Fully penetrant XO lethal. XX is WT. Enhancer of her-1(sd), tra-2(lf), tra-3(lf) XX masculinization phenotypes. Does not enhance tra-1(lf).