Strain: AA1 Species: Caenorhabditis elegans Genotype: daf-12(rh257) X. Description: daf-d. Strong heterochronic phenotypes in seam, somatic gonad, intestine. Class I allele. Occasional abnormal dauers under exhausted conditions. Mutagen: EMS Outcrossed: x Made by: Adam Antebi Received: 10/23/00 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AA10 Species: Caenorhabditis elegans Genotype: daf-12(rh286) X. Description: Weak heterochronic phenotypes in seam, intestine, somatic gonad. Class V allele. Mutagen: EMS Outcrossed: x4 Made by: Adam Antebi Received: 10/23/00 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AA107 Species: Caenorhabditis elegans Genotype: nhr-48(ok178) X. Description: ZK662.3 Homozygous. No obvious phenotype. Outer left primer sequence: TCTGAAGTTTGTGAGCCGTG. Outer right primer sequence: AGCGCCTAGATGAGCAACAT. Inner left primer sequence: TCCGTTGAATGCCATCTGTA. Inner right primer sequence: GGACGATGCACATGAGTTTG. Inner primer PCR product length: 3324 bp. Deletion size: 1956 bp. Mutagen: UV/TMP Outcrossed: x2 Made by: Adam Antebi Received: 08/19/03 Massachusetts General Hospital, Charlestown, MA ----------------------------------------------------------------------------- Strain: AA120 Species: Caenorhabditis elegans Genotype: dhIs26. Description: dhIs26 [daf-12a::GFP + lin-15(+)]. DAF-12::GFP localized primarily in nucleus, except during mitosis. Expressed widely in most cells including tissues modified for dauer formation or by stage from embryo to adult, but most elevated and widespread during L2. Mutagen: UV Outcrossed: x3 Made by: C. Kober-Eisermann Received: 03/23/07 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AA18 Species: Caenorhabditis elegans Genotype: daf-12(rh61rh412) X. Description: daf-d. Weak heterochronic phenotypes in seam, somatic gonad and intestine. Class III allele. Mutagen: Spontaneous Outcrossed: x Made by: Adam Antebi Received: 11/03/00 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AA277 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; dhIs64. Description: dhIs64 [daf-9p::daf-9::GFP + lin-15(+)]. Mutagen: Outcrossed: x Made by: Antebi Lab Received: 01/11/13 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AA278 Species: Caenorhabditis elegans Genotype: dhIs59. Description: dhIs59 [Topo::daf-9::GFP + lin-15(+)]. Perinuclear expression in a ventral pair of bilateral neurons identified as the IL1Vs or URAVs in the anterior ganglia. By mid-L2, expression in the cytoplasm of the hypodermis, the syncitial epidermis, but absent from midline, epidermal seam cells. Levels peak around the L2 molt and diminish during L4. In some cases, transient expression seen in the L3 vulval blast cells. Also expressed within the hermaphrodite spermatheca starting in late L4 larvae and continuing eve in old adults. In males, expression in IL1V/URAVs and hypodermis but not somatic gonad. In dauer larvae, strong expression in IL1V/URAV and specifically extends into axonal but not dendritic processes. In post-dauer stages, expression in a pattern similar to reproductively growing animals, except expression is absent in the hypodermis. Grow at 20C. May still contain lin-15(n765) mutation in the background. Mutagen: UV Outcrossed: x3 Made by: C. Kober-Eisermann Received: 03/23/07 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AA292 Species: Caenorhabditis elegans Genotype: daf-36(k114) V. Description: Mig on low cholesterol. Single daf-c at 27C, weak Mig. Strong expression in intestine at all stages. Grow at 20C. Mutagen: Outcrossed: x Made by: Veerle Rottiers Received: 03/23/07 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AA34 Species: Caenorhabditis elegans Genotype: daf-12(rh61) X. Description: daf-d. Strong heterochronic phenotypes in seam, somatic gonad, intestine. Class I allele. Mutagen: EMS Outcrossed: x2 Made by: Ed Hedgecock Received: 10/23/00 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AA408 Species: Caenorhabditis elegans Genotype: din-1(dh127) II. Description: daf-d, suppresses daf-12(rh61) daf-12(rh274) gonadal migration defects. Mutagen: EMS Outcrossed: x3 Made by: Andreas Ludewig Received: 01/28/08 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AA411 Species: Caenorhabditis elegans Genotype: din-1(dh149) II. Description: daf-d, suppresses daf-12(rh61) daf-12(rh274) gonadal migration defects. Mutagen: EMS Outcrossed: x3 Made by: Andreas Ludewig Received: 01/28/08 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AA426 Species: Caenorhabditis elegans Genotype: dre-1(dh99) V. Description: Precocious fusion of seam cells one stage earlier (prior to L3 molt); impenetrant gonadal migration defects; SynMig on daf-12 RNAi. Mutagen: EMS Outcrossed: x3 Made by: Nicole Fielenbach Received: 04/02/07 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AA6 Species: Caenorhabditis elegans Genotype: daf-12(rh84) X. Description: daf-d. Strong heterochronic phenotypes in seam, somatic gonad, intestine. Class I allele. Mutagen: EMS Outcrossed: x2 Made by: Ed Hedgecock Received: 10/23/00 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AA699 Species: Caenorhabditis elegans Genotype: din-1(hd36) II. Description: non-Daf. Temperature-sensitive phenotypes: at 20C half of the animals are egg-laying defective with occasional mispositioned gonadal arms; at 25C, 18% arrest as embryos: those animals that hatch usually display variable morphology defects in body and pharynx; nearly all animals that live to adults are small, clear, slightly uncoordinated, constipated, and virtually sterile. Maintain at 20C or below. Mutagen: Outcrossed: x Made by: Harald Hutter Received: 01/28/08 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AA776 Species: Caenorhabditis elegans Genotype: cyp-44A1(ok216) II. Description: Mutagen: UV/TMP Outcrossed: x0 Made by: OMRF Knockout Group Received: 07/22/03 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AA790 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; dhEx343. Description: dhEx343 [din-1p::din-1E::GFP + lin-15(+)]. Pick GFP+ to maintain. Animals with the array are GFP+ and non-Muv. Animals which have lost the array are Muv and non-GFP. din-1s::GFP is detected in hypodermis, seam, intestine, and somatic gonad including the distal tip cells. din-1s is also expressed in neurons, vulval precursors, body wall muscle, pharynx, and all tissues with heterochronic phenotypes or remodeled during dauer. Expression is first detected in a few nuclei by the comma stage of embryogenesis. By hatching, din-1s was widely expressed, albeit weakly. Overall expression in most tissues is detected at various levels into adult and in dauer larvae. Animals with the array are GFP+ and non-Muv. Animals which have lost the array are Muv and non-GFP. din-1p::din-1E::GFP was produced by cloning into Fire Lab vector L3781. Mutagen: Outcrossed: x Made by: Kerstin Neubert Received: 07/02/07 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AA82 Species: Caenorhabditis elegans Genotype: daf-12(rh284) X. Description: Gonadal lead cell Mig. Weak heterochronic phenotype in intestine. Weakly daf-c at 25C. Class V allele. Mutagen: EMS Outcrossed: x2 Made by: Adam Antebi Received: 10/23/00 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AA83 Species: Caenorhabditis elegans Genotype: daf-12(rh62rh157) X. Description: daf-d. Strong heterochronic phenotypes in seam and intestine. Weak heterochronic phenotypes in somatic gonad. Class II allele. Mutagen: Spontaneous revertant Outcrossed: x3 Made by: Ed Hedgecock Received: 10/23/00 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AA85 Species: Caenorhabditis elegans Genotype: daf-12(rh285) X. Description: Strong heterochronic phenotypes in seam, somatic gonad, and intestine. Weakly daf-c at 15C. Class IV allele. Mutagen: EMS Outcrossed: x2 Made by: Adam Antevi Received: 11/03/00 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AA86 Species: Caenorhabditis elegans Genotype: daf-12(rh61rh411) X. Description: Daf-d, weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele. Mutagen: Spontaneous revertant Outcrossed: x2 Made by: Adam Antebi Received: 10/23/00 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AA87 Species: Caenorhabditis elegans Genotype: daf-12(rh273) X. Description: Daf-c, gonadal Mig, weak heterochronic phenotypes in intestine and seam. Class VI allele. Mutagen: EMS Outcrossed: x3 Made by: Adam Antebi Received: 10/23/00 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AA88 Species: Caenorhabditis elegans Genotype: daf-12(rh193) X. Description: Strong heterochronic phenotypes in seam, somatic gonad, and intestine. Heterochronic phenotypes less penetrant at 15C. Weakly daf-c at 25C. Class IV allele. Mutagen: Outcrossed: x3 Made by: Adam Antebi Received: 11/03/00 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AA89 Species: Caenorhabditis elegans Genotype: daf-12(rh274) X. Description: daf-c. Gonadal Mig. Weak heterochronic phenotypes in intestine. Class VI allele. Mutagen: EMS Outcrossed: x3 Made by: Adam Antebi Received: 11/03/00 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AA968 Species: C.elegans Genotype: nhr-8(hd117) IV. Description: Mig on low cholesterol. Reference: Magner DB, et al. Cell Metab. 2013 Aug 6;18(2):212-24. doi: 10.1016/j.cmet.2013.07.007.PMID: 23931753 Mutagen: EMS Outcrossed: x4 Made by: Harald Hutter Received: 07/11/22 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: AB1 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Reference WBG 10(2) 140-141 and WBG 8(2) 52. Caenorhabditis elegans wild isolate. (Tc1 pattern VII). Mutagen: Outcrossed: x Made by: Received: 09/01/84 CSIRO Adelaide, Australia ----------------------------------------------------------------------------- Strain: AB2 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Reference WBG 10(2) 140-141 and WBG 8(2) 52. Caenorhabditis elegans wild isolate. (Tc1 pattern VIII). Mutagen: Outcrossed: x Made by: Received: 09/01/84 CSIRO Adelaide, Australia ----------------------------------------------------------------------------- Strain: AB3 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Reference WBG 10(2) 140-141 and WBG 8(2) 52. Caenorhabditis elegans wild isolate. (Tc1 pattern VIII). Mutagen: Outcrossed: x Made by: Received: 09/01/84 CSIRO Adelaide, Australia ----------------------------------------------------------------------------- Strain: AB4 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Reference WBG 10(2) 140-141 and WBG 8(2) 52. Caenorhabditis elegans wild isolate. (Tc1 pattern VIII). Mutagen: Outcrossed: x Made by: Received: 09/01/84 CSIRO Adelaide, Australia ----------------------------------------------------------------------------- Strain: ABR1 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; staEx1. Description: staEx1 [T20F7.6p + pha-1(+)]. Empty vector control strain. Maintain at 25 degrees. Superficially wild-type. Reference: Greer EL et al Curr Biol 2007 Oct 9;17(19):1646-56. Mutagen: Outcrossed: x0 Made by: Eric Greer Received: 04/22/10 STANFORD UNIVERSITY ----------------------------------------------------------------------------- Strain: ABR14 Species: Caenorhabditis elegans Genotype: shEx34. Description: shEx34 [myo-3p::mCherry]. Pick mCherry+ to maintain. This strain serves as a control strain to ABR16. Reference: Han S, et al. Nature 2017 doi: 10.1038/nature21686. Mutagen: Outcrossed: x0 Made by: Shuo Han Received: 03/30/17 STANFORD UNIVERSITY ----------------------------------------------------------------------------- Strain: ABR156 Species: C. briggsae Genotype: Cbr-she-1(v35) IV; mfIs42. Description: mfIs42 [Cel-sid-2(+) + Cel-myo-2::dsRed]. Maintain at 15C. Feminization is partially-penetrant at 15C; most hermaphrodites are somewhat self-fertile and can lay small broods. Can be maintained by crossing with male siblings. Feminized C. briggsae strain made susceptible to RNAi knock-down by feeding dsRNA due to the transgenic expression of C. elegans SID-2. Generated by crossing parental strains JU1018 with RE665. Reference: Booth LN, eLife 2019 Jul 8;8:e46418. PMID: 31282863. Mutagen: Outcrossed: x0 Made by: Lauren Booth Received: 10/13/21 STANFORD UNIVERSITY ----------------------------------------------------------------------------- Strain: ABR16 Species: Caenorhabditis elegans Genotype: shEx1. Description: shEx1 [ges-1p::fat-7 + myo-3p::mCherry]. Pick mCherry+ to maintain. FAT-7 over-expressing strain. ABR14 serves as a control strain for this strain. Reference: Han S, et al. Nature 2017 doi: 10.1038/nature21686. Mutagen: Outcrossed: x0 Made by: Shuo Han Received: 03/30/17 STANFORD UNIVERSITY ----------------------------------------------------------------------------- Strain: ABR161 Species: C. elegans Genotype: hjIs37; ldrIs1. Description: hjIs37 [vha-6p::mRFP-PTS1 + Cbr-unc-119(+)]. ldrIs1 [dhs-3p::dhs-3::GFP + unc-76(+)]. mRFP targeted to peroxisomes in intestinal cells. dhs-3::GFP is expressed mainly in intestinal cells and localized to intestinal lipid droplets. Derived by crossing parental strains VS10 and LIU1 and outcrossing six times to ABR lab stock of N2. Reference: Papsdorf K, et al. Nat Cell Biol. 2023 May;25(5):672-684. doi: 10.1038/s41556-023-01136-6. 2023. PMID 37127715. Mutagen: Outcrossed: x6 Made by: Katharina Papsdorf Received: 08/31/23 STANFORD UNIVERSITY ----------------------------------------------------------------------------- Strain: ABR212 Species: C. elegans Genotype: acd-1(sta6) delm-2(ok1822) I. Description: acd-1 and delm-2 are tandem paralogs. This double mutant was created by CRISPR-engineered deletion of acd-1 in a delm-2(ok1822) background (parental strain RB1523). acd-1(sta6) is predicted to be a null allele (~200bp indel causing frameshift in exon 4). Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Lauren Booth Received: 10/13/21 STANFORD UNIVERSITY ----------------------------------------------------------------------------- Strain: ABR225 Species: C. elegans Genotype: acd-1(sta6) delm-2(ok1822) I; delm-1(ok1266) IV. Description: acd-1 and delm-2 are tandem paralogs. This double mutant was created by CRISPR-engineered deletion of acd-1 in a delm-2(ok1822) background (parental strain RB1523). acd-1(sta6) is predicted to be a null allele (~200bp indel causing frameshift in exon 4). This triple mutant strain was made by crossing the acd-1(sta6) delm-2(ok1822) double mutant with delm-1(ok1226) parental strain RB1177. Mutagen: none Outcrossed: x0 Made by: Lauren Booth Received: 10/13/21 STANFORD UNIVERSITY ----------------------------------------------------------------------------- Strain: ABR339 Species: C. elegans Genotype: lpin-1(wbm76[lpin-1::GFP]) V. Description: GFP tag inserted into endogenous lpin-1 locus. The strain was generated by using 5' attgttgctggcatcaaaaa crRNA for C-terminal lpin-1 editing and using dpy-10 editing as a co-conversion marker, followed by outcrossing twice to ABR lab stock of N2 to eliminate the dpy-10 co-conversion marker. Reference: Papsdorf et al, Nature Cell Biology, 2023, PMID 37127715. [NOTE: This strain was incorrectly named WBM1369 lpin-1(sta10[lpin-1::GFP]) in an earlier version of the paper.] Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Carlos G Silva-García/Katharina Papsdorf Received: 08/31/23 STANFORD UNIVERSITY ----------------------------------------------------------------------------- Strain: ABR4 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; staEx4. Description: staEx4 [T20F7.6p(R81Q)::T20F7.6 + pha-1(+)]. Constitutively active T20f7.6 promoter construct (CA3). Maintain at 25 degrees. Superficially wild-type with increased lifespan and stress resistance. Reference: Greer EL et al Curr Biol 2007 Oct 9;17(19):1646-56. Mutagen: Outcrossed: x0 Made by: Eric Greer Received: 04/22/10 STANFORD UNIVERSITY ----------------------------------------------------------------------------- Strain: ABR5 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; staIs1. Description: staIs1 [pie-1p::GFP + unc-119(+)]. Superficially wild-type. Maintain under normal conditions. Reference: This strain was used as the empty vector control in Greer EL et al Nature 2010 doi: 10.1038/nature09195. Mutagen: Bombardment Outcrossed: x0 Made by: Eric Greer Received: 03/15/25 STANFORD UNIVERSITY ----------------------------------------------------------------------------- Strain: ABR7 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; staIs2. Description: staIs2 [pie-1p::rbr-2::GFP + unc-119(+)]. Extended longevity. Maintain under normal conditions. Reference: This strain was used as LC Ppie-1::rbr-2::GFP (#3) in Greer EL et al Nature 2010 doi: 10.1038/nature09195. Mutagen: Bombardment Outcrossed: x0 Made by: Eric Greer Received: 03/15/25 STANFORD UNIVERSITY ----------------------------------------------------------------------------- Strain: ABR9 Species: Caenorhabditis elegans Genotype: set-2(ok952) III; rbr-2(tm1231) IV. Description: Reduced lifespan. Maintain under normal conditions. The parental rbr-2 strain was outcrossed 6x and the parental set-2 strain was outcrossed 2x. Reference: Greer EL et al Nature (2010) doi: 10.1038/nature09195. Mutagen: Outcrossed: x Made by: Eric Greer Received: 06/17/10 STANFORD UNIVERSITY ----------------------------------------------------------------------------- Strain: AC196 Species: Caenorhabditis elegans Genotype: sao-1(ik1) V. Description: Superficially wild-type. Suppresses of aph-1(zu147). Reference: Hale VA, et al. Genetics. 2012 Mar; 190(3): 1043-1057. Mutagen: EMS Outcrossed: x5 Made by: C. Goutte & V. Hale Received: 07/20/15 Amherst College, Amherst, MA ----------------------------------------------------------------------------- Strain: AC257 Species: Caenorhabditis elegans Genotype: ppk-3(n2668) X. Description: Growth retardation, enlarged vacuoles (late endosomes and lysosomes) in intestine, epidermis, coelomocytes and pharynx. 27% embryonic lethality and 8% post-embryonic lethality. Mutagen: EMS Outcrossed: x8 Made by: Andrew Chisholm Received: 04/25/07 UMR7622, IBPS, Paris, France ----------------------------------------------------------------------------- Strain: AC365 Species: Caenorhabditis elegans Genotype: sao-1(ok3335) V. Description: Derived by outcrossing parental strain RB2429 six times to N2, followed by recombining flanking chromosome to the right and left by recombining on, and then off rol-4(sc8) and unc-76(e911). Reference: Hale VA, et al. Genetics. 2012 Mar; 190(3): 1043-1057. Mutagen: EMS Outcrossed: x13 Made by: Valerie Hale Received: 07/20/15 Amherst College, Amherst, MA ----------------------------------------------------------------------------- Strain: AC68 Species: Caenorhabditis elegans Genotype: unc-29(e1072) aph-2(zu181)/unc-13(e1091) lin-11(n566) I. Description: Heterozygotes are WT and segregate WT, Unc Egls, and dead eggs. Mutagen: Tc1 Outcrossed: x10 Made by: Received: 11/09/00 Amherst College, Amherst, MA ----------------------------------------------------------------------------- Strain: AD186 Species: Caenorhabditis elegans Genotype: egg-1(tm1071) III. Description: Temperature sensitive sterile. Maintain at 20C. Fertility is <10% of WT at 25C. Mutagen: UV/TMP Outcrossed: x>3 Made by: S. Mitani Received: 01/17/06 Waksman Institute, Piscataway, NJ ----------------------------------------------------------------------------- Strain: AD189 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; asIs2. Description: asIs2 [pie-1p::GFP::egg-1 + unc-119(+)]. Oocyte membranes are GFP+. Mutagen: Microparticle Bombardment Outcrossed: x Made by: Received: 03/27/06 Waksman Institute, Piscataway, NJ ----------------------------------------------------------------------------- Strain: AD200 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; asIs1. Description: asIs1 [pie-1p::GFP::egg-3 + unc-119(+)]. Mutagen: Outcrossed: x3 Made by: Rika Maruyama Received: 03/20/08 Waksman Institute, Piscataway, NJ ----------------------------------------------------------------------------- Strain: AD213 Species: Caenorhabditis elegans Genotype: spe-19(eb52) V; asEx83. Description: asEx83 [spe-19(+) + myo-3p::GFP]. Pick GFP+ to maintain. asEx83 contains 7.3kb genomic fragment including spe-19 (Y113G7A.10) and 850bp of upstream sequence. Transgene rescues spe-19(eb52) sperm activation defect. GFP+ hermaphrodites are fertile. non-GFP hermaphrodites are sterile. All males are fertile. Mutagen: EMS Outcrossed: x Made by: Bryan Geldziler Received: 02/12/16 Waksman Institute, Piscataway, NJ ----------------------------------------------------------------------------- Strain: AD226 Species: Caenorhabditis elegans Genotype: egg-3(tm1191)/mIn1 [mIs14 dpy-10(e128)] II. Description: Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ (heterozygotes), Dpy bright GFP+ (mIn1 homozygotes), and non-GFP tm1191 homozygotes. Pick WT dim GFP and check for correct segregation of progeny to maintain. Mutagen: Outcrossed: x3 Made by: Japanese KO Consort. Received: 03/20/08 Waksman Institute, Piscataway, NJ ----------------------------------------------------------------------------- Strain: AD238 Species: Caenorhabditis elegans Genotype: asIs2. Description: asIs2 [pie-1p::mCherry::egg-3]. Mutagen: Outcrossed: x3 Made by: Rika Maruyama Received: 03/20/08 Waksman Institute, Piscataway, NJ ----------------------------------------------------------------------------- Strain: AD265 Species: Caenorhabditis elegans Genotype: nnIs2. Description: nnIs2 [pie-1p::GFP::chs-1 + unc-119(+)]. Mutagen: Outcrossed: x3 Made by: Rika Maruyama Received: 06/25/08 Waksman Institute, Piscataway, NJ ----------------------------------------------------------------------------- Strain: AD266 Species: Caenorhabditis elegans Genotype: egg-4(tm1508) egg-5(ok1781) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP tm1508 ok1781 homozygotes (maternal sterile). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Parry JM, et al 2009 Current Biology 19(20):1752-7. Mutagen: Outcrossed: x Made by: Jean Parry Received: 12/15/09 Waksman Institute, Piscataway, NJ ----------------------------------------------------------------------------- Strain: AD271 Species: Caenorhabditis elegans Genotype: spe-38(eb44) I; him-5(e1490) V; asEx78. Description: asEx78 [spe-38p::spe-38(cDNA)::spe-38 3'UTR + myo-3p::GFP]. Pick GFP+ to maintain. GFP+ worms are fertile; animals that have lost the array are sterile. Mutagen: Outcrossed: x Made by: G. Singaravelu Received: 06/01/11 Waksman Institute, Piscataway, NJ ----------------------------------------------------------------------------- Strain: AD281 Species: Caenorhabditis elegans Genotype: spe-45(as38) IV; him-5(e1490) V. Description: Him. Temperature-sensitive sterile. Small brood size even at permissive temperatures; pick fertile animals and maintain at 15C. Worms lacking spe-45 function produce morphologically normal and motile sperm that cannot fuse with oocytes despite direct contact in the reproductive tract. spe-45 hermaphrodites and males are subfertile at 16C and sterile at 25C. Reference: Singaravelu G, et al. Current Biology 2015. http://dx.doi.org/10.1016/j.cub.2015.10.055 Mutagen: EMS Outcrossed: x10 Made by: G. Singaravelu Received: 01/20/16 Waksman Institute, Piscataway, NJ ----------------------------------------------------------------------------- Strain: AD292 Species: C. elegans Genotype: spe-51(as39) IV; him-5(e1490) V; asEx95. Description: asEx95 [T22B11.1(genomic) + myo-3p::GFP]. Pick GFP+ animals to maintain. as39 is a non-conditional allele of spe-51. Mutant hermaphrodites and males are severely subfertile due to a sperm defect. The extrachromosomal array asEx95 effectively rescues the fertility defect. Him. Reference: Mei X, et al. Curr Biol. 2023 Jul 3;S0960-9822(23)00780-7. doi: 10.1016/j.cub.2023.06.029. PMID: 37453427. Mutagen: EMS Outcrossed: x>3 Made by: Xue Mei Received: 08/15/23 St. John's University ----------------------------------------------------------------------------- Strain: AD294 Species: C. elegans Genotype: cylc-2(mon2[cylc-2::mNG::3xFLAG) I; him-5(e1490) V. Description: Endogenous cycl-2 locus tagged with mNeonGreen (mNG). Green fluorescence in sperm. Him. Reference: Krauchunas AR, et al. (2020). C. elegans CYLC-2 localizes to sperm. microPublication Biology. 10.17912/micropub.biology.000314. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Amy Maddox Received: 09/24/21 University of Delaware ----------------------------------------------------------------------------- Strain: AD296 Species: C. elegans Genotype: spe-51(syb2737[spe-51::mNeonGreen]) IV; him-5(e1490) V. Description: mNeonGreen tag inserted into the endogenous spe-51 locus. Him. Reference: Mei X, et al. Curr Biol. 2023 Jul 3;S0960-9822(23)00780-7. doi: 10.1016/j.cub.2023.06.029. PMID: 37453427. Mutagen: Outcrossed: x1 Made by: Xue Mei Received: 08/15/23 St. John's University ----------------------------------------------------------------------------- Strain: AD319 Species: C. elegans Genotype: spe-38(syb6556[spe-38::wrmScarlet-I]) I; him-5(e1490) V. Description: wrmScarlet-I tag inserted into endogenous spe-38 locus. Him. wrmScarlet-I expression labels membranous organelles (MOs) in the sperm. Reference: Zuo Y, et al. Biomolecules. 2023; 13(4):623. https://doi.org/10.3390/biom13040623 Mutagen: Outcrossed: x3 Made by: SunyBiotech & Yamei Zuo Received: 05/22/23 Waksman Institute, Piscataway, NJ ----------------------------------------------------------------------------- Strain: ADS1002 Species: C. elegans Genotype: aeaIs10. Description: aeaIs10 [rgef-1p::GCaMP6s::3xNLS + lin-15(+)]. Worms express GCaMP6s in all neuronal nuclei. Pan-neuronal imaging strain; suitable for rapid whole-brain imaging due to brightness, good signal to noise ratio, and relative resistance to photo-bleaching. Reference: Susoy V, et al. Cell. 2021 Sep 30;184(20):5122-5137.e17. PMID: 34534446 Mutagen: Outcrossed: x3 Made by: Zhen and Samuel labs Received: 12/08/21 Harvard University Physics Department ----------------------------------------------------------------------------- Strain: ADS707 Species: Caenorhabditis elegans Genotype: unc-13(s69) I; aeaIs8; hpIs728. Description: aeaIs8 [ift-20p::GCaMP6s::3xNLS + lin-15(+)]. hpIs728 [gpc-1p::wCherry + lin-15(+)]. Unc. Nuclear-localized GCaMP6s expressed in ciliated sensory neurons. Cytoplasmic wCherry expression in a subset of neurons. Derived by crossing EG9631 (unc-13) hermaphrodites with ZM10104 (aeaIs8; hpIs728) heterozygous males. Reference: Lin A, et al. bioRxiv 2022.05.27.493772; doi: https://doi.org/10.1101/2022.05.27.493772. Mutagen: N/A Outcrossed: x0 Made by: Albert Lin Received: 02/20/23 Harvard University Physics Department ----------------------------------------------------------------------------- Strain: AE501 Species: Caenorhabditis elegans Genotype: nhr-8(ok186) IV. Description: Approx. 1.3 kb deletion - does not remove entire coding region, might not be null allele. No overt morphological or behavioral abnormalities. Homozygotes are 2-3X more sensitive to colchicine and chloroquine than are N2 animals. Mutagen: EMS Outcrossed: x4 Made by: Moulder/Lindblom Received: 08/30/01 Massachusetts General Hospital, Charlestown, MA ----------------------------------------------------------------------------- Strain: AF1 Species: Caenorhabditis elegans Genotype: +/szT1 [lon-2(e678)] I; dpy-8(e1321) unc-3(e151)/szT1 X. Description: Heterozygotes are WT and segregate WT, DpyUnc, dead eggs and Lon males. Maintain by picking WT. Mutagen: X-ray Outcrossed: x Made by: Fodor A Received: 05/01/81 Hungarian Academy of Science, Szeged ----------------------------------------------------------------------------- Strain: AF13(4) Species: Oscheius akosreti Genotype: Oscheius akosreti wild isolate. Description: Isolated in Memorial Park in Madison, WI. No hermaphrodites. Lots of SDS resistant dauers. Can survive between 15-28C, but grows very slowly at 15C. Mutagen: Outcrossed: x Made by: Andras Fodor Received: 08/21/95 Hungarian Academy of Science, Szeged ----------------------------------------------------------------------------- Strain: AF16 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: See WBG 7(1) 48. Isolated from soil in Ahmedabad, India. Previously called C. briggsae G16. Mutagen: Outcrossed: x Made by: Andras Fodor Received: 02/01/82 Hungarian Academy of Science, Szeged ----------------------------------------------------------------------------- Strain: AF25 Species: Unknown species Genotype: Unknown species wild isolate Description: Mutagen: Outcrossed: x Made by: Andras Fodor Received: 08/21/95 Hungarian Academy of Science, Szeged ----------------------------------------------------------------------------- Strain: AF271 Species: Unknown species Genotype: Unknown species wild isolate Description: Mutagen: Outcrossed: x Made by: Andras Fodor Received: 08/21/95 Hungarian Academy of Science, Szeged ----------------------------------------------------------------------------- Strain: AF272 Species: Unknown species Genotype: Unknown species wild isolate Description: Dark in color. Mutagen: Outcrossed: x Made by: Andras Fodor Received: 08/21/95 Hungarian Academy of Science, Szeged ----------------------------------------------------------------------------- Strain: AF36 Species: Panagrolaimus rigidus Genotype: Panagrolaimus rigidus wild isolate. Description: Panagrolaimus rigidus (Schneider, 1886). Males and females. Animals are long. Mutagen: Outcrossed: x Made by: Andras Fodor Received: 08/21/95 Hungarian Academy of Science, Szeged ----------------------------------------------------------------------------- Strain: AF40 Species: Panagrolaimus rigidus Genotype: Panagrolaimus rigidus wild isolate. Description: Panagrolaimidae: P. rigidus (Schneider, 1866). Dig themselves into the agar. Mutagen: Outcrossed: x Made by: Andras Fodor Received: 08/21/95 Hungarian Academy of Science, Szeged ----------------------------------------------------------------------------- Strain: AF5 Species: Oscheius sp. Genotype: Oscheius sp. wild isolate Description: Isolated in Towers Hill State Park in WI. Males are rare. No SDS resistant dauers. Animals are osmosensitive, can be dried competely and recover in water. Rhabditidae. Previously known as Rhabditis sp. Mutagen: Outcrossed: x Made by: Andras Fodor Received: 08/21/95 Hungarian Academy of Science, Szeged ----------------------------------------------------------------------------- Strain: AF6733 Species: Unknown species Genotype: Unknown species wild isolate. Description: Isolated in Pennsylvania. SDS resistant dauers. Males are rare. Mutagen: Outcrossed: x Made by: Andras Fodor Received: 08/21/95 Hungarian Academy of Science, Szeged ----------------------------------------------------------------------------- Strain: AF6840 Species: Unknown species Genotype: Unknown species wild isolate. Description: Isolated in Pennsylvania. Males are rare. Mutagen: Outcrossed: x Made by: Andras Fodor Received: 08/21/95 Hungarian Academy of Science, Szeged ----------------------------------------------------------------------------- Strain: AF72 Species: Mesorhabditis spiculigera Genotype: Mesorhabditis spiculigera wild isolate. Description: Mesorhabditis spiculigera. Isolated in Pennsylvania. NOTE: (06/10/2016) AF72 was originally described as Mesorhabditis miotki, but K. Kiontke has determined through morphological inspection that this is actually a strain of Mesorhabditis spiculigera. Mutagen: Outcrossed: x Made by: Andras Fodor Received: 08/21/95 Hungarian Academy of Science, Szeged ----------------------------------------------------------------------------- Strain: AF7340 Species: Unknown species Genotype: Unknown species wild isolate Description: Isolated in Ohio. Mutagen: Outcrossed: x Made by: Andras Fodor Received: 08/21/95 Hungarian Academy of Science, Szeged ----------------------------------------------------------------------------- Strain: AF78 Species: Mesorhabditis sp. Genotype: Mesorhabditis sp. wild isolate. Description: Rhabditidae: Mesorhabditis sp. Isolated in Governor Dodge State Park in South Dakota. Dark in color. Mutagen: Outcrossed: x Made by: Andras Fodor Received: 08/21/95 Hungarian Academy of Science, Szeged ----------------------------------------------------------------------------- Strain: AF8032 Species: Unknown species Genotype: Unknown species wild isolate Description: Isolated at Niagara Falls in Canada. SDS resistant dauers look like C. elegans predauers. Males are rare. Mutagen: Outcrossed: x Made by: Andras Fodor Received: 08/21/95 Hungarian Academy of Science, Szeged ----------------------------------------------------------------------------- Strain: AF8130 Species: Pristionchus sp. Genotype: Pristionchus sp. wild isolate. Description: Neodiplogasteridae: Pristionchus Iheritieri, Maupas, 1919. Isolated in Ontario, Canada. [9/98: Ralf Sommer has found this strain to be hermaphroditic. He had successful matings between AF8130 and PS312 Pristionchus pacificus, indicating that AF8130 is probably P. pacificus.] Mutagen: Outcrossed: x Made by: Andras Fodor Received: 08/21/95 Hungarian Academy of Science, Szeged ----------------------------------------------------------------------------- Strain: AFS205 Species: Caenorhabditis elegans Genotype: zen-4(cle5) IV. Description: Temperature-sensitive embryonic-lethal mutant. Maintain at 15C. Shift L4s to 25C overnight to observe mutant phenotype of embryos produced by adults. Mutants lack a central spindle during early embryonic mitosis and exhibits a late cytokinesis defect (cleavage furrows regress after ingressing in nearly to the center of dividing embryonic cells). This strain can be used for CRISPR-Cas9 co-conversion. There is a causal mis-sense mutation present in zen-4(cle5), GAC to AAC (D520N), and one silent mutation, GCA to GCT at codon 519, that introduces an AluI site for RFLP analysis. A previous deposited version of this strain, zen-4(ok153), possessed two mis-sense mutations: GAC to AAC (D520N) and GAT to AAT (D735N). Reference: Farboud B, et al. Genetics Early online November 30, 2018; https://doi.org/10.1534/genetics.118.301775. Mutagen: Crispr/Cas9 Outcrossed: x4 Made by: Aaron F. Severson Received: 12/05/18 Cleveland State University, Cleveland OH, USA ----------------------------------------------------------------------------- Strain: AFS222 Species: Caenorhabditis elegans Genotype: zen-4(cle10) IV. Description: Temperature-sensitive embryonic-lethal mutant. Maintain at 15C. Shift L4s to 25C overnight to observe mutant phenotype of embryos produced by adults. Mutants lack a central spindle during early embryonic mitosis and exhibits a late cytokinesis defect (cleavage furrows regress after ingressing in nearly to the center of dividing embryonic cells). This strain can be used for CRISPR-Cas9 co-conversion. There is a causal mis-sense mutation present in zen-4(cle10), GAC to AAC (D520N), and two silent mutations. One silent mutation is a CGA to CGG mutation at codon 523 that creates a recognition site for a Cas9 guide RNA, in order to use zen-4(cle10ts) as a CRISPR/Cas9 co-conversion marker. The other silent mutation is a GCA to GCT mutation at codon 519 that introduces an AluI site for RFLP analysis. Reference: Farboud B, et al. Genetics Early online November 30, 2018; https://doi.org/10.1534/genetics.118.301775. Mutagen: Crispr/Cas9 Outcrossed: x5 Made by: Aaron F. Severson Received: 12/05/18 Cleveland State University, Cleveland OH, USA ----------------------------------------------------------------------------- Strain: AG150 Species: Caenorhabditis elegans Genotype: apc-1(ar104)/unc-4(e120) bli-1(e769) II. Description: Heterozygotes are WT and segregate WT, UncBli, and Steriles which have an everted vulva. ar104 previously called evl-22 and mat-2. Mutagen: Outcrossed: x Made by: Andy Golden Received: 05/30/01 NIDDK/NIH ----------------------------------------------------------------------------- Strain: AG151 Species: Caenorhabditis elegans Genotype: cdc-25.1(nr2036)/dpy-5(e61) unc-13(e450) I. Description: Heterozygotes are WT and segregate WT, DpyUncs and adult steriles. Original deletion from Axys Pharmaceuticals. Mutagen: UV/TMP Outcrossed: x10 Made by: Ashcroft/Golden Received: 01/24/03 NIDDK/NIH ----------------------------------------------------------------------------- Strain: AG152 Species: Caenorhabditis elegans Genotype: unc-85(e1414) bli-2(e768) dpy-10(e128) II. Description: Unc and Dpy. Not Blistered: dpy-10 suppresses the appearance of the blisters. Mutagen: Outcrossed: x Made by: Andy Golden Received: 06/17/05 NIDDK/NIH ----------------------------------------------------------------------------- Strain: AG165 Species: Caenorhabditis elegans Genotype: san-1(av31) unc-13(e450) I. Description: Unc. Reduced brood size. Larval lethal, larval arrest. Steriles. Bursts at vulva. Can be maintained as a homozygote. Mutagen: EMS Outcrossed: x3 Made by: Ed Davis Received: 05/01/07 NIDDK/NIH ----------------------------------------------------------------------------- Strain: AG166 Species: Caenorhabditis elegans Genotype: mdf-2(av16) unc-17(e245) IV. Description: Reduced brood size. Reduced hatching. Slow growth. Larval lethal. Larval arrest. Bursts at vulva. Suppresses the mat-3 one-cell arrest at 25C. Mutagen: EMS Outcrossed: x3 Made by: Received: 05/09/07 NIDDK/NIH ----------------------------------------------------------------------------- Strain: AG168 Species: Caenorhabditis elegans Genotype: fzy-1(av15) unc-4(e120) II. Description: Unc. Gain-of-function allele of fzy-1. Mutagen: EMS Outcrossed: x Made by: Andy Golden Received: 06/06/07 NIDDK/NIH ----------------------------------------------------------------------------- Strain: AG171 Species: Caenorhabditis elegans Genotype: mdf-1(av19) unc-42(e270) V. Description: Unc. Previously called AG164 by the CGC. Mutagen: EMS Outcrossed: x Made by: Andy Golden Received: 05/01/07 NIDDK/NIH ----------------------------------------------------------------------------- Strain: AG175 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; avEx122. Description: avEx122 [(pAG126) lpin-1p::GFP::lpin-1::lpin-1 3'UTR + unc-119(+)]. No GFP expression detected in germline. Mutagen: Bombardment Outcrossed: x Made by: Orna Cohen-Fix Received: 07/02/09 NIH, NIDDK, Bethesda, MD ----------------------------------------------------------------------------- Strain: AG176 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; avEx123. Description: avEx123 [lpin-1p::GFP::his-58::lpin-1 3'UTR + unc-119(+)]. No GFP expression detected in germline. Mutagen: Bombardment Outcrossed: x Made by: Orna Cohen-Fix Received: 07/02/09 NIH, NIDDK, Bethesda, MD ----------------------------------------------------------------------------- Strain: AG226 Species: Caenorhabditis elegans Genotype: rol-6(e187) unc-4 (e120)/mnC1 [dpy-10(e128) unc-52(e444) nIs190 let-?] II; him-8(e1489) IV. Description: nIs190 [myo-2::GFP]. Him. Heterozygotes are wild-type GFP+ and segregate WT GFP+ heterozygotes, Rol Uncs, dead embryos, and males. nIs190 [myo-2::GFP] integrated in or near mnC1. Approx 0.5% recombination seen between nIs190 and mnC1. Fails to complemement all markers on mnC1. Mutagen: Outcrossed: x10 Made by: Andy Golden Received: 09/05/13 NIDDK/NIH ----------------------------------------------------------------------------- Strain: AG247 Species: Caenorhabditis elegans Genotype: tyms-1(tm2429) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Homozygous lethal deletion balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP+, arrested hT2 aneuploids, and non-GFP tm2429 homozygotes (embryonic lethal). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP+ and check for correct segregation of progeny to maintain. Reference: Jaramillo-Lambert A, et al. G3 (2015). Mutagen: Outcrossed: x Made by: Abby Fuchsman Received: 03/27/15 NIDDK/NIH ----------------------------------------------------------------------------- Strain: AG248 Species: Caenorhabditis elegans Genotype: mus-101(tm1761) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Homozygous lethal deletion balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP+, arrested hT2 aneuploids, and non-GFP tm1761 homozygotes (embryonic lethal). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP+ and check for correct segregation of progeny to maintain. Reference: Jaramillo-Lambert A, et al. G3 (2015). Mutagen: Outcrossed: x Made by: Abby Fuchsman Received: 03/27/15 NIDDK/NIH ----------------------------------------------------------------------------- Strain: AG400 Species: C. elegans Genotype: fasn-1(av138[fasn-1::gfp]) I. Description: Homozygous viable, gfp expression in intestine, hypodermis, developing vulva, somatic gonad. Reference: Starich et al. eLife 2020;9:e58619. DOI: https://doi.org/10.7554/eLife.58619 Mutagen: Outcrossed: x2 Made by: Xiaofei Bai Received: 03/21/23 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: AG406 Species: C. elegans Genotype: pezo-1(av144) IV. Description: av144 is a CRISPR/Cas9 engineered deletion in the N-terminal region of pezo-1 removing exons 1–13. Small brood size. Reference: Bai X, et al. Elife. 2020 Jun 3:9:e53603. doi: 10.7554/eLife.53603. PMID: 32490809. Mutagen: Outcrossed: x0 Made by: Xiaofei Bai Received: 09/13/24 University of Florida ----------------------------------------------------------------------------- Strain: AG416 Species: C. elegans Genotype: pezo-1(av149) IV. Description: av149 is a CRISPR/Cas9 engineered deletion in the C-terminal region of pezo-1 removing the last seven exons (27–33) and introns. Small brood size. Reference: Bai X, et al. Elife. 2020 Jun 3:9:e53603. doi: 10.7554/eLife.53603. PMID: 32490809. Mutagen: Outcrossed: x0 Made by: Xiaofei Bai Received: 09/13/24 University of Florida ----------------------------------------------------------------------------- Strain: AG50 Species: Caenorhabditis elegans Genotype: daf-7(e1372) dpy-1(e1) III. Description: Maintain at 15C. Temperature-sensitive dauer constitutive: 100% dauers at 25C, leaky at 20C. Dpy. Crowds. Mutagen: Outcrossed: x Made by: Andy Golden Received: 08/27/99 NIDDK/NIH ----------------------------------------------------------------------------- Strain: AGD1032 Species: Caenorhabditis elegans Genotype: glp-1(e2141) III; xzEx1. Description: xzEx1 [unc-54p::Dendra2]. Maintain at 15C; sterile at 25C. Pick animals with green fluorescence in body wall muscle to maintain. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8. Mutagen: Outcrossed: x0 Made by: David Vilchez Received: 11/22/13 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD1033 Species: Caenorhabditis elegans Genotype: glp-1(e2141) III; xzEx3. Description: xzEx3 [unc-54p::UbG76V::Dendra2]. Maintain at 15C; sterile at 25C. Pick animals with green fluorescence in body wall muscle to maintain. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8. Mutagen: Outcrossed: x0 Made by: David Vilchez Received: 11/22/13 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD1047 Species: Caenorhabditis elegans Genotype: glp-1(e2141) III; uthEx649. Description: uthEx649 [rpn-6p::tdTomato + rol-6(su1006)]. Temperature-sensitive. Maintain at 15C; sterile at 25C. Rollers. Pick rollers to maintain array. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8. Mutagen: Outcrossed: x0 Made by: David Vilchez Received: 04/25/14 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD1048 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; glp-1(e2141) III; uthEx649. Description: uthEx649 [rpn-6p::tdTomato + rol-6(su1006)]. Temperature-sensitive. Maintain at 15C; sterile at 25C. Rollers. Pick rollers to maintain array. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8. Mutagen: Outcrossed: x0 Made by: David Vilchez Received: 04/25/14 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD1101 Species: Caenorhabditis elegans Genotype: uthIs372. Description: uthIs372 [sur-5p::pat-10::unc-54 3'UTR + myo-2p::tdTomato::unc-54 3' UTR]. Long-lived, thermotolerant. Reference: Baird NA, et al. Science. 2014 Oct 17;346(6207):360-3. Mutagen: Outcrossed: x7 Made by: Nathan Baird Received: 05/28/15 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD383 Species: Caenorhabditis elegans Genotype: uthIs202. Description: uthIs202 [aak-2(intron 1)::aak-2(aa1-aa321)::Tomato::unc-54 3'UTR + rol-6(su1006)]. Pick Rollers to maintain. Reference: Mair W, et al. Nature. 2011 Feb 17;470(7334):404-8. Mutagen: Outcrossed: x4 Made by: Will Mair Received: 09/18/13 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD397 Species: Caenorhabditis elegans Genotype: aak-1(tm1944) III; aak-2(ok524) X; uthEx202. Description: uthEx202 [crtc-1p::crtc-1 cDNA::tdTomato::unc-54 3'UTR + rol-6(su1006)]. Pick Rollers to maintain. Reference: Mair W, et al. Nature. 2011 Feb 17;470(7334):404-8. Mutagen: Outcrossed: x Made by: Will Mair Received: 09/18/13 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD418 Species: Caenorhabditis elegans Genotype: uthIs205. Description: uthIs205 [crtc-1p::crtc-1::RFP::unc-54 3'UTR + rol-6(su1006)]. Mutagen: Outcrossed: x4 Made by: William Mair Received: 08/17/12 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD448 Species: Caenorhabditis elegans Genotype: uthEx488. Description: uthEx488 [crtc-1p::crtc-1 cDNA (S179A)::tdTomato + rol-6(su1006)]. Pick Rollers to maintain. Reference: Mair W, et al. Nature. 2011 Feb 17;470(7334):404-8. Mutagen: Outcrossed: x Made by: Will Mair Received: 09/18/13 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD466 Species: Caenorhabditis elegans Genotype: uthEx222. Description: uthEx222 [crtc-1p::crtc-1 cDNA (S76A, S179A)::tdTomato::unc-54 3'UTR + rol-6(su1006)]. Pick Rollers to maintain. Reference: Mair W, et al. Nature. 2011 Feb 17;470(7334):404-8. Mutagen: Outcrossed: x Made by: Will Mair Received: 09/18/13 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD467 Species: Caenorhabditis elegans Genotype: uthEx490. Description: uthEx490 [aak-2 (intron 1)::aak-2(aa1-aa321 T172D)::GFP + crtc-1p::crtc-1::tdTomato + rol-6(su1006)]. Pick Rollers to maintain. Reference: Mair W, et al. Nature. 2011 Feb 17;470(7334):404-8. Mutagen: Outcrossed: x Made by: Will Mair Received: 09/18/13 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD469 Species: Caenorhabditis elegans Genotype: uthEx492. Description: uthEx492 [aak-2 (intron 1)::aak-2(aa1-aa321 T172A)::GFP + crtc-1p::crtc-1::tdTomato + rol-6(su1006)]. Pick Rollers to maintain. Reference: Mair W, et al. Nature. 2011 Feb 17;470(7334):404-8. Mutagen: Outcrossed: x Made by: Will Mair Received: 09/18/13 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD470 Species: Caenorhabditis elegans Genotype: uthEx493. Description: uthEx493 [crtc-1p::crtc-1 cDNA (S76A)::tdTomato + rol-6(su1006)]. Pick Rollers to maintain. Reference: Mair W, et al. Nature. 2011 Feb 17;470(7334):404-8. Mutagen: Outcrossed: x Made by: Will Mair Received: 09/18/13 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD597 Species: Caenorhabditis elegans Genotype: uthEx556. Description: uthEx556 [sur-5p::rpn-6 + myo-3p::GFP]. Pick animals with GFP expression in body wall muscle to maintain. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8. Mutagen: Outcrossed: x0 Made by: David Vilchez Received: 09/18/13 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD598 Species: Caenorhabditis elegans Genotype: uthEx557. Description: uthEx557 [sur-5p::rpn-6 + myo-3p::GFP]. Pick animals with GFP expression in body wall muscle to maintain. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8. Mutagen: Outcrossed: x Made by: David Vilchez Received: 09/18/13 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD614 Species: Caenorhabditis elegans Genotype: uthEx633. Description: uthEx633 [myo-3p::GFP]. Pick animals with GFP expression in body wall muscle to maintain. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8. Mutagen: Outcrossed: x0 Made by: David Vilchez Received: 09/18/13 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD710 Species: Caenorhabditis elegans Genotype: uthIs235. Description: uthIs235 [sur-5p::hsf-1::unc-54 3'UTR + myo-2p::tdTomato::unc-54 3' UTR]. Long-lived, thermotolerant. Small brood size. Reference: Baird NA, et al. Science. 2014 Oct 17;346(6207):360-3. Mutagen: Outcrossed: x2+ Made by: Nathan Baird Received: 05/29/15 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD731 Species: Caenorhabditis elegans Genotype: uthEx299. Description: uthEx299 [aak-2 (genomic aa1-aa321)::GFP::unc-54 3'UTR + myo-2p::tdTomato]. Pick animals with red pharynx to maintain. Reference: Mair W, et al. Nature. 2011 Feb 17;470(7334):404-8. Mutagen: Outcrossed: x Made by: Will Mair Received: 09/18/13 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD794 Species: Caenorhabditis elegans Genotype: hsf-1 (sy441) I; uthIs225. Description: uthIs225 [sur5p::hsf-1(CT-Delta)::unc-54 3'UTR + myo-2p::tdTomato::unc-54 3' UTR]. Long-lived, thermotolerant. Small brood size. Reference: Baird NA, et al. Science. 2014 Oct 17;346(6207):360-3. Mutagen: Outcrossed: x2+ Made by: Nathan Baird Received: 05/28/15 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD850 Species: Caenorhabditis elegans Genotype: rmIs110; uthEx557. Description: rmIs110 [F25B3.3p::Q40::YFP]. uthEx557 [sur5p::rpn-6 + myo3p::GFP]. All animals will express YFP in nervous system; pick animals with GFP expression in body wall muscle to maintain. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8. Mutagen: Outcrossed: x0 Made by: David Vilchez Received: 11/22/13 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD851 Species: Caenorhabditis elegans Genotype: rmIs284; uthEx557. Description: rmIs284 [F25B3.3p::Q67::YFP]. uthEx557 [sur-5p::rpn-6 + myo-3p::GFP]. All animals will express YFP in nervous system; pick animals with GFP expression in body wall muscle to maintain. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8. Mutagen: Outcrossed: x Made by: David Vilchez Received: 09/18/13 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD866 Species: Caenorhabditis elegans Genotype: rmIs110; uthEx633. Description: rmIs110 [F25B3.3p::Q40::YFP]. uthEx633 [myo-3p::GFP]. All animals will express YFP in nervous system; pick animals with GFP expression in body wall muscle to maintain. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8. Mutagen: Outcrossed: x Made by: David Vilchez Received: 09/18/13 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD867 Species: Caenorhabditis elegans Genotype: rmIs284; uthEx633. Description: rmIs284 [F25B3.3p::Q67::YFP]. uthEx633 [myo-3p::GFP]. All animals will express YFP in nervous system; pick animals with GFP expression in body wall muscle to maintain. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8. Mutagen: Outcrossed: x Made by: David Vilchez Received: 09/18/13 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD885 Species: Caenorhabditis elegans Genotype: rrf-3(b26) II; fem-1(hc17) IV; uthEx633. Description: uthEx633 [myo-3p::GFP]. Maintain at 15C; sterile at 25C. Pick animals with GFP expression in body wall muscle to maintain. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8. Mutagen: Outcrossed: x Made by: David Vilchez Received: 09/18/13 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD886 Species: Caenorhabditis elegans Genotype: rrf-3(b26) II; fem-1(hc17) IV; uthEx557. Description: uthEx557 [sur-5p::rpn-6 + myo-3p::GFP]. Maintain at 15C; sterile at 25C. Pick animals with GFP expression in body wall muscle to maintain. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8. Mutagen: Outcrossed: x Made by: David Vilchez Received: 11/22/13 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD926 Species: Caenorhabditis elegans Genotype: zcIs4 V; uthIs269. Description: zcIs4 [hsp-4::GFP] V. uthIs269 [sur-5p::hsf-1::unc-54 3'UTR + myo-2p::tdTomato::unc-54 3' UTR]. ER stress resistence. Reference: Taylor RC, Dillin A. Cell. 2013 Jun 20;153(7):1435-47. Mutagen: Outcrossed: x5+ Made by: Rebecca Taylor Received: 05/28/15 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD927 Species: Caenorhabditis elegans Genotype: uthIs270. Description: uthIs270 [rab-3p::xbp-1s (constitutively active) + myo-2p::tdTomato]. Pick animals with red pharynx to maintain. Reference: Taylor RC, Dillin A. Cell. 2013 Jun 20;153(7):1435-47. Mutagen: Outcrossed: x8 Made by: Rebecca Taylor Received: 09/18/13 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD945 Species: Caenorhabditis elegans Genotype: uthEx649. Description: uthEx649 [rpn-6p::tdTomato + rol-6(su1006)]. Rollers. Pick rollers to maintain array. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8. Mutagen: Outcrossed: x0 Made by: David Vilchez Received: 04/25/14 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGD946 Species: Caenorhabditis elegans Genotype: uthEx650. Description: uthEx650 [rpn-6p::tdTomato + rol-6(su1006)]. Rollers. Pick rollers to maintain array. Reference: Vilchez D, et al. Nature. 2012 Sep 13;489(7415):263-8. Mutagen: Outcrossed: x0 Made by: David Vilchez Received: 04/25/14 University of California Berkeley, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: AGK192 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; zdIs13 IV; armIs5. Description: zdIs13 [tph-1p::GFP] IV. armIs5 [zfp-1(fosmid)::FLAG + unc-119(+)]. Integrated zfp-1 transgene expressed in the germline. Fosmid-based zfp-1::FLAG transgene fully rescues stress-sensitivity and reduced lifespan in zfp-1(ok554) homozygotes. ChIP with anti-FLAG antibody detects ZFP-1::FLAG localization to promoters of highly expressed genes. References: Mansisidor AR, et al. PLoS Genet. 2011 Sep;7(9):e1002299. Avgousti DC, et al. Mol Cell Biol. 2013 Mar;33(5):999-1015. Cecere G, et al. Mol Cell. 2013 Jun 27;50(6):894-907. Mutagen: Outcrossed: x0 Made by: Cecere & Kennedy Received: 11/20/13 Boston University School of Medicine, Boston, MA ----------------------------------------------------------------------------- Strain: AGK233 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; niDf199 IV; armEx58. Description: armEx58 [WRM0611aH08-Del8mer + unc-119(+)]. Pick non-Unc to maintain. This strain contains a transgenic array that expresses a derivative WRM0611aH08 fosmid. The WRM0611aH08 fosmid contains the niDF199 locus (around 4 kb) that is deleted in the natural C. elegans isolate strain JU258. JU258 worms lack specific 21U-RNAs normally present in N2 worms due to this deletion of the niDF199 locus. This derivative fosmid construct lacks the upstream 8-mer motif (CTGTTTCA) next to 21U-3372. The expression of this individual 21U-RNA is lost in transgenic animals. unc-119(ed3) was crossed into JU258, the niDf199IV deletion was confirmed by PCR, and these Unc worms were used for bombardment. Reference: Cecere G, et al. Mol Cell. 2012 Sep 14;47(5):734-45. Mutagen: Outcrossed: x0 Made by: Cecere & Mansisidore Received: 12/16/14 Boston University School of Medicine, Boston, MA ----------------------------------------------------------------------------- Strain: AGK234 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; niDf199 IV; armEx53. Description: armEx53 [WRM0611aH08 + unc-119(+)]. Pick non-Unc to maintain. unc-119(ed3) was crossed into JU258, the niDf199IV deletion was confirmed by PCR, and these Unc worms were used for bombardment. This strain contains a transgenic array that expresses the WRM0611aH08 fosmid construct. This fosmid contains the niDF199 locus (around 4 kb) that is deleted in the natural C. elegans isolate strain JU258. JU258 worms lack specific 21U-RNAs normally present in N2 worms due to this deletion of the niDF199 locus. Expression of this fosmid construct in JU258 worms restores the expression of the missing 21U-RNAs in the germline, as measured by RT-qPCR. Reference: Cecere G, et al. Mol Cell. 2012 Sep 14;47(5):734-45. Mutagen: Outcrossed: x0 Made by: Cecere & Mansisidore Received: 12/16/14 Boston University School of Medicine, Boston, MA ----------------------------------------------------------------------------- Strain: AGK26 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; armEx5. Description: armEx5 [zfp-1(fosmid)::GFP + unc-119(+)]. Pick non-Unc to maintain. Fosmid-based zfp-1::GFP transgene fully rescues stress-sensitivity and reduced lifespan in zfp-1(ok554) homozygotes. Nuclear expression of zfp-1::GFP is observed ubiquitously in somatic cells in all developmental stages; high levels of GFP expression is observed in oocytes with lower levels of expression in the distal germline. References: Mansisidor AR, et al. PLoS Genet. 2011 Sep;7(9):e1002299. Avgousti DC, et al. Mol Cell Biol. 2013 Mar;33(5):999-1015. Mutagen: Outcrossed: x0 Made by: Cecere & Mansisidore Received: 11/20/13 Boston University School of Medicine, Boston, MA ----------------------------------------------------------------------------- Strain: AGK280 Species: Caenorhabditis elegans Genotype: zfp-1(ok554) unc-119(ed3) III; armEx14. Description: armEx14 [PHD1-PHD2::FLAG + zfp-1(short isoform) + unc-119(+)]. Pick non-Unc animals to maintain. The fosmid-based armEx14 transgene rescues zfp-1(ok554)/nDf17 lethality. Reference: Avgousti DC, et al. Mol Cell Biol. 2013 Mar;33(5):999-1015. Mutagen: Outcrossed: x0 Made by: Cecere & Mansisidore Received: 11/20/13 Boston University School of Medicine, Boston, MA ----------------------------------------------------------------------------- Strain: AGK369 Species: Caenorhabditis elegans Genotype: zfp-1(ok554) III; armIs8. Description: armIs8 [zfp-1(short isoform)::FLAG::GFP + rol-6(su1006)]. Rollers. The fosmid-based armIs8 transgene rescues the protruded vulva phenotype of zfp-1(ok554). Ubiquitous nuclear localization of zfp-1(long isoform)::FLAG::GFP is observed in somatic cells in all developmental stages, but is silenced in the germline. See AGK26 for germline-expressing zfp-1::GFP. Reference: Avgousti DC, et al. Mol Cell Biol. 2013 Mar;33(5):999-1015. Mutagen: Outcrossed: x Made by: Received: 12/16/14 Boston University School of Medicine, Boston, MA ----------------------------------------------------------------------------- Strain: AGK370 Species: Caenorhabditis elegans Genotype: zfp-1(ok554) III; armIs9. Description: armIs9 [zfp-1(long isoform)::FLAG::GFP + rol-6(su1006)]. Rollers. The fosmid-based armIs9 transgene rescues zfp-1(ok554)/nDf17 lethality. Ubiquitous nuclear localization of zfp-1(long isoform)::FLAG::GFP is observed in somatic cells in all developmental stages, but is silenced in the germline. See AGK26 for germline-expressing zfp-1::GFP. Reference: Avgousti DC, et al. Mol Cell Biol. 2013 Mar;33(5):999-1015. Mutagen: Outcrossed: x2 Made by: Daphne Avgousti Received: 11/20/13 Boston University School of Medicine, Boston, MA ----------------------------------------------------------------------------- Strain: AGK532 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; niDf199 IV; armEx196. Description: armEx196 [mex-5p::unc-130::GFP::tbb-2 3'UTR + Cbr-unc-119(+)]. Pick non-Unc to maintain. unc-119(ed3) was crossed into JU258, the niDf199IV deletion was confirmed by PCR, and these Unc worms were used for bombardment. JU258 worms lack specific 21U-RNAs normally present in N2 worms due to deletion of the niDF199 locus. Reference: Cecere G, et al. Mol Cell. 2012 Sep 14;47(5):734-45. Reference: Cecere G, et al. Mol Cell. 2012 Sep 14;47(5):734-45. Mutagen: Outcrossed: x0 Made by: Germano Cecere Received: 12/16/14 Boston University School of Medicine, Boston, MA ----------------------------------------------------------------------------- Strain: AGK537 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; armEx199. Description: armEx199 [cdl-1p::cdl-1::GFP + unc-119(+)]. Pick non-Unc to maintain. Nuclear localization of CDL-1::GFP in the germline and early embryos; strong enrichement of CDL-1::GFP in the nuclei of developing oocytes. Reference: Avgousti DC, et al. EMBO J. 2012 Oct 3;31(19):3821-32. Mutagen: Outcrossed: x0 Made by: Daphne Avgousti Received: 03/15/25 Boston University School of Medicine, Boston, MA ----------------------------------------------------------------------------- Strain: AGK541 Species: Caenorhabditis elegans Genotype: armSi1 II; unc-119(ed3) III. Description: armSi1 [mex5p::unc-130::GFP::tbb-2 3'UTR + Cbr-unc-119(+)] II. GFP expression from transgene is observed in the germline. Reference: Cecere G, et al. Mol Cell. 2012 Sep 14;47(5):734-45. Mutagen: Outcrossed: x0 Made by: Germano Cecere Received: 12/16/14 Boston University School of Medicine, Boston, MA ----------------------------------------------------------------------------- Strain: AGK573 Species: Caenorhabditis elegans Genotype: otIs225 II; daf-18(ok480) IV; armEx218. Description: otIs225 [cat-4::GFP] II. armEx218 [unc-119p::daf-18 + unc-119p::tagRFP + rol-6(su1006)]. Pick Rollers to maintain. Transgenic array expresses DAF-18 from unc-119 pan-neuronal promoter; rescues the HSN under-migration phenotype in daf-18 null mutants. Reference: Kennedy LM, et al. Cell Rep. 2013 Sep 12;4(5):996-1009. Mutagen: Outcrossed: x0 Made by: Lisa Kennedy Received: 12/16/13 Boston University School of Medicine, Boston, MA ----------------------------------------------------------------------------- Strain: AGK587 Species: Caenorhabditis elegans Genotype: armEx227. Description: armEx227 [pak-1p::NLS::tagRFP + rol-6(su1006)]. Pick rollers to maintain. pak-1p::NLS::tagRFP is expressed primarily in the hypodermal tissue during the comma and 1.5-fold stages, and in the CAN cells at the 3-fold stage through adulthood. Expression can also be seen in additional neurons during the larval and adult stages. Reference: Kennedy LM, et al. Cell Rep. 2013 Sep 12;4(5):996-1009. Mutagen: Outcrossed: x Made by: Received: 12/16/13 Boston University School of Medicine, Boston, MA ----------------------------------------------------------------------------- Strain: AGK640 Species: Caenorhabditis elegans Genotype: zdIs13 IV; pak-1(ok448) X; armEx252. Description: zdIs13 [tph-1p::GFP] IV. armEx252 [dpy-7p::pak-1::tagRFP + myo-2::GFP]. Pick animals with GFP+ pharynx to maintain. armEx252 rescues the pak-1(ok448) HSN under-migration phenotype. pak-1::tagRFP is expressed in the hypodermal tissue throughout development and adulthood. Reference: Kennedy LM, et al. Cell Rep. 2013 Sep 12;4(5):996-1009. Mutagen: Outcrossed: x0 Made by: Lisa Kennedy Received: 12/16/13 Boston University School of Medicine, Boston, MA ----------------------------------------------------------------------------- Strain: AGK650 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; zdIs13 IV; armEx257. Description: zdIs13 [tph-1p::GFP] IV. armEx257 [dpy-7p::daf-16b::tagRFP + myo-2::GFP]. Pick animals with GFP+ pharynx to maintain. armEx257 rescues the daf-16(mu86) HSN undermigration phenotype. dpy-7p::daf-16b::tagRFP expression is localized to nuclei in hypodermal tissue during the comma, 1.5 and 2-fold stages, becoming cytoplasmic or perinuclear by the 3-fold stage and persisting into adulthood. Reference: Kennedy LM, et al. Cell Rep. 2013 Sep 12;4(5):996-1009. Mutagen: Outcrossed: x0 Made by: Lisa Kennedy Received: 12/16/13 Boston University School of Medicine, Boston, MA ----------------------------------------------------------------------------- Strain: AH102 Species: Caenorhabditis elegans Genotype: lip-1(zh15) IV. Description: Deletion allele which removes exons 2 to 6 of lip-1 (C05B10.1). Incompletely penetrant ovulation defect. Mutagen: EMS Outcrossed: x8 Made by: Thomas Berset Received: 05/21/01 University of Zurich, Zurich, Switzerland ----------------------------------------------------------------------------- Strain: AH12 Species: Caenorhabditis elegans Genotype: gap-1(ga133) X. Description: Null allele. Mutagen: Psoralen Outcrossed: x4 Made by: Alex Hajnal Received: 12/08/97 University of Zurich, Zurich, Switzerland ----------------------------------------------------------------------------- Strain: AH142 Species: Caenorhabditis elegans Genotype: zhIs4 III. Description: zhIs4 [lip-1::GFP] III. lip-1::GFP transcriptional reporter expression is upregulated in the secondary VPCs P5.p and P7.p of early L3 animals. Mutagen: Gamma Rays Outcrossed: x5 Made by: Thomas Berset Received: 05/21/01 University of Zurich, Zurich, Switzerland ----------------------------------------------------------------------------- Strain: AH159 Species: Caenorhabditis elegans Genotype: sra-13(zh13) II. Description: sra-13(zh13) mutants display stronger chemotaxis to limiting concentrations of isoamylalcohol and diacetyl than WT animals. Deletion allele. 396 bp of 5' promoter sequence and all but the last exon are removed; probably a null allele. Mutagen: EMS Outcrossed: x8 Made by: Gopal Battu Received: 12/16/03 University of Zurich, Zurich, Switzerland ----------------------------------------------------------------------------- Strain: AH1747 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; zhIs35 I. Description: zhIs35 [let-23::GFP + unc-119(+)] I. zhIs35 resuces let-23(sy1) and recapitulates LET-23 antibody staining in VPCs. let-23::GFP transgene expression is higher in this strain than in AH1779 unc-119(ed3) III; zhIs38. Reference: Haag A, et al. PLoS Genet. 2014 May 1;10(5):e1004341. Mutagen: Bombardment Outcrossed: x2 Made by: JM Escobar-Restrepo Received: 02/18/16 University of Zurich, Zurich, Switzerland ----------------------------------------------------------------------------- Strain: AH1779 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; zhIs38 IV. Description: zhIs38 [let-23::GFP + unc-119(+)] IV. zhIs38 resuces let-23(sy1) and recapitulates LET-23 antibody staining in VPCs. let-23::GFP transgene is expressed at levels similar to endogenous LET-23. Reference: Haag A, et al. PLoS Genet. 2014 May 1;10(5):e1004341. Mutagen: Bombardment Outcrossed: x2 Made by: JM Escobar-Restrepo Received: 02/18/16 University of Zurich, Zurich, Switzerland ----------------------------------------------------------------------------- Strain: AH205 Species: Caenorhabditis elegans Genotype: sdn-1(zh20) X. Description: Slightly Unc. Variably Egl. zh20 is a deletion in sdn-1. The sequence of the breakpoint is: TTTGCTTCACAC//zh20//GTCGACAGGCAG. Mutagen: EMS Outcrossed: x6 Made by: Erika Frohli Received: 10/26/05 University of Zurich, Zurich, Switzerland ----------------------------------------------------------------------------- Strain: AH286 Species: Caenorhabditis elegans Genotype: unc-4(e120) ect-2(zh8) II; gap-1(ga133) X. Description: Muv and Unc. Semi-dominant mutation in ect-2 (previously called let-21). Mutagen: EMS Outcrossed: x3 Made by: Erika Frohli Received: 12/27/05 University of Zurich, Zurich, Switzerland ----------------------------------------------------------------------------- Strain: AH3437 Species: C. elegans Genotype: tln-1(zh117[gfp::tln-1]) I. Description: Wild-type morphology. Endogenous GFP reporter for tln-1. Reference: Walser M, et al. PLoS Genet. 2017 Jan 30;13(1):e1006592. Mutagen: Outcrossed: x3 Made by: Erika Fröhli Received: 01/23/20 University of Zurich, Zurich, Switzerland ----------------------------------------------------------------------------- Strain: AH346 Species: Caenorhabditis elegans Genotype: dep-1(zh34) unc-4(e120) II; lip-1(zh15) IV. Description: Pvl and weak Muv. Transformation of secondary to primary vulval cell fates. Mutagen: Outcrossed: x Made by: Thomas Berset Received: 12/27/05 University of Zurich, Zurich, Switzerland ----------------------------------------------------------------------------- Strain: AH5059 Species: Caenorhabditis elegans Genotype: let-23(zh131[FRT::let-23::FRT::GFP::LoxP::FLAG::let-23]) II. Description: CRISPR allele of endogenous let-23, which expresses let-23::GFP fusion protein and is susceptible for conditional knock-out via incorporated FRT sites with FLPase expression. Reference: Konietzka et al. 2019. Current Biology (accepted). Mutagen: Outcrossed: x4 Made by: Silvan Spiri Received: 02/28/20 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: AH75 Species: Caenorhabditis elegans Genotype: apr-1(zh10) unc-29(e1072) I; zhEx11. Description: zhEx11[apr-1(+) + sur-5::GFP]. Unc. Segregates dead eggs that have lost the rescuing array. Mutagen: EMS Outcrossed: x6 Made by: Alex Hajnal Received: 07/24/00 University of Zurich, Zurich, Switzerland ----------------------------------------------------------------------------- Strain: AL132 Species: C. elegans Genotype: icIs132. Description: icIs132 [unc-40::GFP]. Superficially wild-type. unc-40 translational reporter. Reference: Chan SS, et al. Cell. 1996 Oct 18;87(2):187-95. Mutagen: none Outcrossed: x- Made by: unknown Received: 09/04/18 Instituto de Biomedicina de Valencia (IBV-CSIC), Valencia, Spain ----------------------------------------------------------------------------- Strain: ALF3 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; daf-12(rh61rh411) X. Description: Daf-d. Unc. Mutagen: Outcrossed: x0 Made by: Yue Zhang Received: 05/01/12 University of Nebraska Medical Center ----------------------------------------------------------------------------- Strain: ALF4 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; daf-12(rh61rh411) X; bafIs4. Description: bafIs4 [daf-12 (fosmid) + unc-119(+)]; rescues both daf-12 and unc-119. Reference: Hochbaum D, et al. PLoS Genet. 2011 Jul;7(7):e1002179. Mutagen: Outcrossed: x0 Made by: Yue Zhang Received: 05/01/12 University of Nebraska Medical Center ----------------------------------------------------------------------------- Strain: ALF62 Species: Caenorhabditis elegans Genotype: bafIs62. Description: bafIs62 [lin-42p::GFP + unc-119(+)]. lin-42p::GFP reporter consists of 2 kb upstream of lin-42a isoform subcloned into modified pPD95.75 vector also carrying unc-119(+). Reference: Hochbaum D, et al. PLoS Genet. 2011 Jul;7(7):e1002179. Mutagen: Bombardment Outcrossed: x4 Made by: Daniel Hochbaum Received: 05/01/12 University of Nebraska Medical Center ----------------------------------------------------------------------------- Strain: ALF63 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; bafIs63. Description: bafIs63 [lin-42p(mut)::GFP + unc-119(+)]. lin-42p(mut)::GFP reporter consists of 2 kb upstream of lin-42a isoform subcloned into modified pPD95.75 vector also carrying unc-119(+); all potential DAF-12 binding sites have been mutated. Reference: Hochbaum D, et al. PLoS Genet. 2011 Jul;7(7):e1002179. Mutagen: Outcrossed: x1 Made by: Daniel Hochbaum Received: 05/01/12 University of Nebraska Medical Center ----------------------------------------------------------------------------- Strain: ALF82 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; daf-12(rh61rh411) X; bafIs62. Description: bafIs62 [lin-42p::GFP + unc-119(+)]. lin-42p::GFP reporter consists of 2 kb upstream of lin-42a isoform subcloned into modified pPD95.75 vector also carrying unc-119(+). Array was crossed into strain ALF3 to create ALF82. Reference: Hochbaum D, et al. PLoS Genet. 2011 Jul;7(7):e1002179. Mutagen: Outcrossed: x0 Made by: Daniel Hochbaum Received: 05/01/12 University of Nebraska Medical Center ----------------------------------------------------------------------------- Strain: ALF9 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; daf-12(rh61rh411) X; bafIs9. Description: bafIs9 [daf-12::TAP (fosmid) + unc-119(+)]; rescues both daf-12 and unc-119. TAP tag inserted into daf-12 fosmid. Reference: Hochbaum D, et al. PLoS Genet. 2011 Jul;7(7):e1002179. Mutagen: Outcrossed: x0 Made by: Yue Zhang Received: 05/01/12 University of Nebraska Medical Center ----------------------------------------------------------------------------- Strain: AM1 Species: Caenorhabditis elegans Genotype: osr-1(rm1) I. Description: Osmotic stress resistant. Received new stock May 7, 2008. Mutagen: EMS Outcrossed: x4 Made by: Aharon Solomon Received: 10/14/04 Northwestern University, Evanston, IL ----------------------------------------------------------------------------- Strain: AM101 Species: Caenorhabditis elegans Genotype: rmIs110. Description: rmIs110 [F25B3.3p::Q40::YFP]. Pan-neuronal YFP expression. Reference: Gidalevitz T, et al., Science. 2006 Mar 10;311(5766):1471-4. Mutagen: gamma irradiation Outcrossed: x0 Made by: Heather Brignull Received: 01/06/11 Northwestern University, Evanston, IL ----------------------------------------------------------------------------- Strain: AM1246 Species: C. elegans Genotype: rmIs126 X. Description: rmIs126 [unc-54p::Q0::YFP]. Diffuse distribution of Q0::YFP throughout the body-wall muscle cells. Worms maintain a soluble phenotype for the duration of their lifespan. [NOTE: This strain is a replacement for AM134, which was removed from distribution due to reports that it did not carry the correct transgene.] Mutagen: gamma irradiation Outcrossed: x5 Made by: Susana Garcia and Alex Chavez Received: 08/29/24 Northwestern University, Evanston, IL ----------------------------------------------------------------------------- Strain: AM138 Species: Caenorhabditis elegans Genotype: rmIs130 II. Description: rmIs130 [unc-54p::Q24::YFP]. Diffuse distribution of Q24::YFP throughout the body-wall muscle cells. Mutagen: Gamma Rays Outcrossed: x5 Made by: S. Garcia Received: 04/05/05 Northwestern University, Evanston, IL ----------------------------------------------------------------------------- Strain: AM140 Species: Caenorhabditis elegans Genotype: rmIs132 I. Description: rmIs132 [unc-54p::Q35::YFP]. AM140 animals show a Q35::YFP progressive transition from soluble to aggregated as they age. Mutagen: Gamma Rays Outcrossed: x5 Made by: S. Garcia/A Chavez Received: 04/05/05 Northwestern University, Evanston, IL ----------------------------------------------------------------------------- Strain: AM141 Species: Caenorhabditis elegans Genotype: rmIs133. Description: rmIs133 [unc-54p::Q40::YFP]. AM141 animals show a soluble Q40::YFP distribution in body wall muscle cells immediately after hatching. As these worms age the rapid formation of foci is observed. When they reach adulthood, AM141 animals show an entirely Q40::YFP aggregated phenotype. Mutagen: Gamma Rays Outcrossed: x5 Made by: S. Garcia/A Chavez Received: 04/05/05 Northwestern University, Evanston, IL ----------------------------------------------------------------------------- Strain: AM263 Species: Caenorhabditis elegans Genotype: rmIs175. Description: rmIs175 [unc-54p::Hsa-sod-1 (WT)::YFP]. Array encodes wild-type human SOD-1. YFP expression in body wall muscle. Array is prone to silencing; maintain by picking worms displaying typical aggregation patterns. Reference: Gidalevitz T, et al., PLoS Genet. 2009 Mar;5(3):e1000399. Mutagen: gamma irradiation Outcrossed: x5 Made by: Tom Krupinski Received: 01/06/11 Northwestern University, Evanston, IL ----------------------------------------------------------------------------- Strain: AM44 Species: Caenorhabditis elegans Genotype: rmIs190. Description: rmIs190 [F25B3.3p::Q67::CFP]. Pan-neuronal CFP expression. Mutagen: gamma irradiation Outcrossed: x5 Made by: S Tang & H Brignull Received: 01/06/11 Northwestern University, Evanston, IL ----------------------------------------------------------------------------- Strain: AM49 Species: Caenorhabditis elegans Genotype: rmIs172. Description: rmIs172 [F25B3.3p::Q19::CFP]. Pan-neuronal CFP expression. Reference: Gidalevitz T, et al., Science. 2006 Mar 10;311(5766):1471-4. Mutagen: gamma irradiation Outcrossed: x5 Made by: S Tang & H Brignull Received: 01/06/11 Northwestern University, Evanston, IL ----------------------------------------------------------------------------- Strain: AM725 Species: Caenorhabditis elegans Genotype: rmIs290. Description: rmIs290 [unc-54p::Hsa-sod-1 (127X)::YFP]. Array encodes mutated form of human SOD-1. YFP expression in body wall muscle. Array is prone to silencing; maintain by picking worms displaying typical aggregation patterns. Reference: Gidalevitz T, et al., PLoS Genet. 2009 Mar;5(3):e1000399. Mutagen: gamma irradiation Outcrossed: x5 Made by: Tom Krupinski Received: 01/06/11 Northwestern University, Evanston, IL ----------------------------------------------------------------------------- Strain: AMH1 Species: C. elegans Genotype: unc-33(e204) IV; sosIs5. Description: sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Paralyzed Unc. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 06/06/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH11 Species: C. elegans Genotype: wpIs36 I; sosIs5. Description: wpIs36 [unc-47p::mCherry] I. sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 06/06/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH13 Species: C. elegans Genotype: wpIs36 I; unc-33(e204) IV; sosIs5. Description: wpIs36 [unc-47p::mCherry] I. sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Paralyzed Unc. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 06/06/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH151 Species: C. elegans Genotype: juIs76 II; daf-7(e1372) III. Description: juIs76 [unc-25p::GFP + lin-15(+)] II. Maintain at 15C. Temperature sensitive dauer constitutive. 100% dauers at 25C. Leaky at 20C. Crowds. Growth slow. GFP expression in GABAergic motor neurons. Mutagen: NA Outcrossed: x0 Made by: Andrea Holgado Received: 10/20/21 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH23 Species: C. elegans Genotype: wpIs36 I; unc-33(e1193) IV; sosIs5. Description: wpIs36 [unc-47p::mCherry] I. sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Unc; almost paralyzed. Growth slow. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 06/06/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH25 Species: C. elegans Genotype: dhc-1(or352) I; sosIs5. Description: sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Maintain at 15 degrees. Temperature-sensitive embryonic lethal. 100% dead embryos at 25 degrees. Small, posteriorly displaced first mitotic spindle with defective chomosome segregation and cytokinesis. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 07/10/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH26 Species: C. elegans Genotype: unc-104(e1265) II; sosIs5. Description: sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Unc. Slow moving. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 07/10/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH3 Species: C. elegans Genotype: unc-33(mn407) IV; sosIs5. Description: sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Unc. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 06/06/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH30 Species: C. elegans Genotype: juIs76 II; daf-2(e1370) III. Description: juIs76 [unc-25p::GFP + lin-15(+)] II. Maintain at 15C. Temperature-sensitive dauer constitutive. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 06/06/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH31 Species: C. elegans Genotype: juIs76 II; unc-33(e1193) IV. Description: juIs76 [unc-25p::GFP + lin-15(+)] II. Unc; almost paralyzed. Growth slow. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 06/06/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH32 Species: C. elegans Genotype: juIs76 II; unc-33(mn407) IV. Description: juIs76 [unc-25p::GFP + lin-15(+)] II. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 06/06/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH34 Species: C. elegans Genotype: juIs76 II; unc-33(e204) IV. Description: juIs76 [unc-25p::GFP + lin-15(+)] II. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 07/10/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH36 Species: C. elegans Genotype: juIs76 II; daf-2(e1370) III; unc-33(mn407) IV. Description: juIs76 [unc-25p::GFP + lin-15(+)] II. Unc. Maintain at 15C. Synthetic Lethality at 25C. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 06/06/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH38 Species: C. elegans Genotype: juIs76 II; daf-2(e1370) III; unc-33(e204) IV. Description: juIs76 [unc-25p::GFP + lin-15(+)] II. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 06/06/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH43 Species: C. elegans Genotype: unc-33(e204) IV; adIs2122. Description: adIs2122 [lgg-1p::GFP::lgg-1 + rol-6(su1006)]. Rollers. Unc. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 06/06/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH46 Species: C. elegans Genotype: juIs76 II; daf-2(e1370) III; unc-33(e1193) IV. Description: Maintain at 15C. Synthetic Lethality at 25C. Unc; almost paralyzed. Growth slow. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 06/06/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH5 Species: C. elegans Genotype: unc-119(ed3) III; sosIs5. Description: sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Mutagen: Gamma Rays Outcrossed: x0 Made by: Andrea Holgado Received: 07/10/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH50 Species: C. elegans Genotype: juIs76; bec-1(ok691) IV/nT1 [qIs51] (IV;V). Description: juIs76 [unc-25p::GFP + lin-15(+)] II.  Heterozygotes are wild-type with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-pharyngeal GFP bec-1 homozygotes. Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 10/08/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH55 Species: C. elegans Genotype: daf-2(e1370) III; otIs117 IV. Description: otIs117 [unc-33p::GFP + unc-4(+)] IV. Maintain at 15C. Temperature-sensitive dauer constitutive. Pan-neuronal GFP. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 07/10/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH57 Species: C. elegans Genotype: unc-33(mn407); olaEx3013. Description: olaEx3013 [ttx-3p:mCherry::eGFP::lgg-1 + unc-122p::mCherry]. Pick animals with mCherry+ coelomocytes to maintain array. Unc. Tandem tags on LGG-1 label immature autophagosomes with both GFP and mCherry, but because GFP is preferentially quenched in an acidic environment, mature structures lose their GFP signal and display solely mCherry signal. Reference: Hill SE & Colon-Ramos D. 2018 bioRxiv 287144; doi: https://doi.org/10.1101/287144 Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 07/10/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH59 Species: C. elegans Genotype: unc-33(e204); olaEx3013. Description: olaEx3013 [ttx-3p:mCherry::eGFP::lgg-1 + unc-122p::mCherry]. Pick animals with mCherry+ coelomocytes to maintain array. Paralyzed Unc. Tandem tags on LGG-1 label immature autophagosomes with both GFP and mCherry, but because GFP is preferentially quenched in an acidic environment, mature structures lose their GFP signal and display solely mCherry signal. Reference: Hill SE & Colon-Ramos D. 2018 bioRxiv 287144; doi: https://doi.org/10.1101/287144 Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 07/10/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH61 Species: C. elegans Genotype: unc-13(e51) I; ddi-1(ok1468) IV. Description: ddi-1 also known as vsm-1. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 06/06/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH67 Species: C. elegans Genotype: unc-33(e204) IV; daf-2(e1370) III. Description: Maintain at 15C. Synthetic Lethality at 25C. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 06/06/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH69 Species: C. elegans Genotype: unc-33(mn407) IV; daf-2(e1370) III. Description: Maintain at 15C. Synthetic Lethality at 25C. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 06/06/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH7 Species: C. elegans Genotype: unc-51(e369) V; sosIs5. Description: sosIs5 [rab-3p::Cerulean-Venus::lgg-1 + unc-119(+)]. Paralyzed Unc. Slow growth. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 10/08/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH71 Species: C. elegans Genotype: unc-33(e1193) IV; daf-2(e1370) III. Description: Maintain at 15C. Synthetic Lethality at 25C. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 06/06/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH73 Species: C. elegans Genotype: ddi-1(ok1468) IV; sosIs6. Description: sosIs6 [C01G5.6p::C01G5.6::GFP]. ddi-1 also known as vsm-1. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 07/10/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH82 Species: C. elegans Genotype: ccIs4251 I; ddi-1(ok1468) IV. Description: ccIs4251 [myo-3p::GFP::LacZ::NLS + myo-3p::mitochondrial GFP + dpy-20(+)] I. ddi-1 also known as vsm-1. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 07/10/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH91 Species: C. elegans Genotype: unc-104(e1265) II; olaEx3013. Description: olaEx3013 [ttx-3p::mCherry::eGFP::lgg-1 + unc-122p::mCherry]. Pick animals with mCherry+ coelomocytes to maintain array. Unc. Slow moving. Tandem tags on LGG-1 label immature autophagosomes with both GFP and mCherry, but because GFP is preferentially quenched in an acidic environment, mature structures lose their GFP signal and display solely mCherry signal. Reference: Hill SE & Colon-Ramos D. 2018 bioRxiv 287144; doi: https://doi.org/10.1101/287144 Mutagen: Outcrossed: x0 Made by: Andrea Holgado Received: 12/17/20 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMH96 Species: C. elegans Genotype: sosIs3. Description: sosIs3 [unc-119p::ddi-1::GFP]. ddi-1 also known as vsm-1. Mutagen: N/A Outcrossed: x0 Made by: Andrea Holgado Received: 07/10/19 St Edward's University, Austin, TX ----------------------------------------------------------------------------- Strain: AMJ345 Species: Caenorhabditis elegans Genotype: jamSi2 II; rde-1(ne219) V. Description: jamSi2 [mex-5p::rde-1(+)] II. mex-5p::rde-1(+) inserted into ttTi5605 on LG II. Tissue-specific RNAi rescue allows silencing in germline and intestine. Reference: Marre JA, et al. Proc Natl Acad Sci USA. 2016. Mutagen: MosSCI Outcrossed: x0 Made by: Julia Marre Received: 09/30/16 University of Maryland, College Park, MD ----------------------------------------------------------------------------- Strain: AML1 Species: Caenorhabditis elegans Genotype: zfIs18; zfIs42. Description: zfIs18 [mec-4p::ChR2::YFP]. zfIs42 [rig-3p::GCaMP3::SL2::mCherry]. Pick mCherry+ animals to maintain strong expression from zfIs42. Worm expresses light-sensitive Channelrhodopsin (ChR2) and yellow fluorescent protein (YFP) in mechanosensory neurons, ALMR/L, AVM, PLML/R, and PVM. When fed all-trans retinal, blue light stimulation (473 nm at 2 mW * mm^-2) of head induces reversals. GCaMP3 and and mCherry expression in command interneurons AVA, and nearby pharyngeal neurons I1, I4, M4, NSM. Worms were made by crossing the integrated strains QW309 and QW625. Reference: Shipley FB, et al. Front Neural Circuits. 2014 Mar 24;8:28. Mutagen: Outcrossed: x0 Made by: Frederick Shipley Received: 05/28/14 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML10 Species: Caenorhabditis elegans Genotype: otIs355; otIs45 V. Description: otIs355 [rab-3::NLS::tagRFP]. otIs45 [unc-119::GFP] V. Pan-neural expression with no injection marker. Reference: Nguyen JP, et al. Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):E1074-81. Mutagen: Outcrossed: x0 Made by: George Plummer Received: 01/30/15 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML105 Species: C. elegans Genotype: wtfIs32. Description: wtfIs32 [str-2p::ChR2(H134R)::GFP +, myo-3p:mCherry]. Expression of an activating opsin molecule ChR2 (H134R) in AWC-ON neuron and mCherry in body wall muscles. Reference: Chen KS, et al. Olfactory learning alters navigation strategies and behavioral variability in C. elegans. ArXiv, Feb 23:arXiv:2311.07117v2. PMID: 38013890. Mutagen: N/A Outcrossed: x6 Made by: Anuj Sharma Received: 07/12/24 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML14 Species: Caenorhabditis elegans Genotype: wtfEx4. Description: wtfEx4 [rab-3p::NLS::GCaMP6s + rab-3p::NLS::tagRFP]. Pick RFP+ to maintain array. Reference: Nguyen JP, et al. Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):E1074-81. Mutagen: Outcrossed: x Made by: G Plummer & S Setu Received: 11/30/15 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML17 Species: Caenorhabditis elegans Genotype: wtfIs2. Description: wtfIs2 [rig-3p::Chrimson::SL2::mCherry]. Transgenic animals express light-gated ion channel Chrimson and fluorescent protein mCherry in AVA neurons as well as nearby nearby pharyngeal neurons I1, I4, M4, NSM. Worms reverse more upon exposure to red light via optogenetic activation of AVA neurons. Reference: Kumar S, et al. PLoS Biol. 2023 Sep 21;21(9):e3002280. doi: 10.1371/journal.pbio.3002280. PMID: 37733772. Mutagen: none Outcrossed: x0 Made by: Mochi Liu Received: 09/29/23 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML175 Species: Caenorhabditis elegans Genotype: lite-1(ce314) X; wtfIs3. Description: wtfIs3 [rab-3p::NLS::GFP + rab-3p::NLS::tagRFP]. Worms expressing the calcium insensitive fluorescent proteins GFP and tagRFP in the nuclei of all neurons in a lite-1(ce314) background. Control strain for AML70. Reference: https://www.biorxiv.org/content/biorxiv/early/2018/10/17/445643.full.pdf Mutagen: Outcrossed: x0 Made by: Anuj Sharma Received: 10/22/18 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML177 Species: C. elegans Genotype: wtfIs145. Description: wtfIs145 [rab-3p::his-24::GCaMP6s::unc-54 3'UTR + pha-1(+)]. Pan-neuronal expression of nuclear-localized GCaMP6s. Reference: Sharma AK, et al. Genetics 2024 May 11:iyae077. doi: 10.1093/genetics/iyae077 PMID: 38733622. Mutagen: N/A Outcrossed: x8 Made by: Anuj Sharma Received: 07/12/24 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML18 Species: Caenorhabditis elegans Genotype: wtfIs3. Description: wtfIs3 [rab-3p::NLS::GFP + rab-3p::NLS::tagRFP]. RFP and GFP expression in the nuclei of all neurons. AML18 acts as a control for the calcium imaging strain AML14. Reference: Nguyen JP, et al. Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):E1074-81. Mutagen: Outcrossed: x2 Made by: George Plummer Received: 11/30/15 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML310 Species: Caenorhabditis elegans Genotype: wtfIs5; wtfEx258. Description: wtfIs5 [rab-3p::NLS::GCaMP6s + rab-3p::NLS::tagRFP]. wtfEx258 [rig-3p::tagBFP::unc-54 3'UTR]. Pick BFP+ animals to maintain transgene. Integrated calcium indicator GCaMP6s and calcium-insensitive fluorescent protein RFP in the nuclei of all neurons for whole brain imaging. BFP expression from rig-3 promoter allows identification of AVAL/R neurons. Reference: Hallinen KM, et al. eLife. 2021 Jul 29;10:e66135. doi: 10.7554/eLife.66135. Mutagen: Outcrossed: x0 Made by: Anuj Sharma Received: 01/21/21 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML318 Species: C. elegans Genotype: otIs669 V. Description: Derived by out-crossing parental strain OH15262 an additional six times to N2. Out-crossed strain AML318 seems healthier than parental strain when maintaining long-term under normal conditions (AML observed an increase in male and sterile progeny in parental strain in successive generations.) otIs669 [UPN::NLS::TagRFP-T + acr-5::NLS::mTagBFP2::H2B + flp-1::NLS::mTagBFP2::H2B + flp-6::NLS::mTagBFP2::H2B + flp-18::NLS::mTagBFP2::H2B + flp-19::NLS::mTagBFP2::H2B + flp-26::NLS::mTagBFP2::H2B + gcy-18::NLS::mTagBFP2::H2B + ggr-3::NLS::mTagBFP2::H2B + lim-4::NLS::mTagBFP2::H2B + pdfr-1::NLS::mTagBFP2::H2B + srab-20::NLS::mTagBFP2::H2B + unc-25::NLS::mTagBFP2::H2B + cho-1::NLS::CyOFP1::H2B + flp-13::NLS::CyOFP1::H2B + flp-20::NLS::CyOFP1::H2B + gcy-36::NLS::CyOFP1::H2B + gpa-1::NLS::CyOFP1::H2B + nlp-12::NLS::CyOFP1::H2B + nmr-1::NLS::CyOFP1::H2B + ocr-1::NLS::CyOFP1::H2B + osm-9::NLS::CyOFP1::H2B + srh-79::NLS::CyOFP1::H2B + sri-1::NLS::CyOFP1::H2B + srsx-3::NLS::CyOFP1::H2B + unc-8::NLS::CyOFP1::H2B + acr-2::NLS::mNeptune2.5 + ceh-2::NLS::mNeptune2.5 + dat-1::NLS::mNeptune2.5 + dhc-3::NLS::mNeptune2.5 + eat-4::NLS::mNeptune2.5 + flp-3::NLS::mNeptune2.5 + gcy-35::NLS::mNeptune2.5 + glr-1::NLS::mNeptune2.5 + gcy-21::NLS::CyOFP1::H2B::T2A::NLS::mTagBFP2::H2B + klp-6::NLS::mNeptune2.5::T2A::NLS::CyOFP1::H2B + lim-6::NLS::mNeptune2.5::T2A::NLS::CyOFP1::H2B + mbr-1::NLS::mNeptune2.5::T2A::NLS::mTagBFP2::H2B + mec-3::NLS::CyOFP1::H2B::T2A::NLS::mTagBFP2::H2B + odr-1::NLS::mNeptune2.5::T2A::NLS::mTagBFP2::H2B + srab-20::NLS::mNeptune2.5::T2A::NLS::mTagBFP2::H2B] V. UPN (Ultra Pan-Neuronal) promoter contains four short pan-neuronal promoters fused together (unc-11::rgef-1::ehs-1::ric-19). NeuroPAL (Neuronal Polychromatic Atlas of Landmarks) transgene used to resolve unique neural identities in whole-brain images. Reference: Yemini E, et al. Cell. 2021 Jan 7;184(1):272-288.e11. PMID: 33378642. Mutagen: N/A Outcrossed: x14 Made by: Anuj Sharma Received: 07/12/24 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML32 Species: Caenorhabditis elegans Genotype: wtfIs5. Description: wtfIs5 [rab-3p::NLS::GCaMP6s + rab-3p::NLS::tagRFP]. Integrated calcium indicator GCaMP6s and calcium-insensitive fluorescent protein RFP in the nuclei of all neurons. Derived from AML14 by integration of wtfEx4. Reference: Nguyen JP, et al. PLoS Comput Biol. 2017 May 18;13(5):e1005517. Mutagen: UV irradiation Outcrossed: x2 Made by: George Plummer Received: 10/20/16 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML320 Species: Caenorhabditis elegans Genotype: otIs669 V; wtfIs145. Description: wtfIs145 [rab-3p::his-24::GCaMP6s::unc-54 3' UTR + pBX]. GCaMP6s transgene allows for imaging whole-brain calcium activity with neuronal identification using NeuroPAL system. See description of strain OH15262 for full description of otIs669 NeuroPAL (Neuronal Polychromatic Atlas of Landmarks) transgene (Yemini E, et al. Cell. 2021 Jan 7;184(1):272-288.e11. PMID: 33378642). Derived by out-crossing parental strain OH15262 an additional six times before incorporating wtfIs145. References: Yu X, et al. Elife. 2021 Jul 14;10:e66410. doi: 10.7554/eLife.66410. PMID: 34259623. Randi F, et al. Nature 2023 Nov;623(7986):406-414. doi: 10.1038/s41586-023-06683-4. PMID: 37914938. Sharma AK, et al. Genetics 2024 May 11:iyae077. doi: 10.1093/genetics/iyae077 PMID: 38733622. Mutagen: none Outcrossed: x6 Made by: Anuj Kumar Sharma Received: 08/24/22 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML344 Species: C. elegans Genotype: juSi164 unc-119(ed3) III; wtfIs263. Description: juSi164 [mex-5p::HIS-72::miniSOG + Cbr-unc-119(+)] III. wtfIs263 [rab-3p::AI::ChR2(H134R)::unc-54 3'UTR + rab-3p::AI::Voltron::unc-54 3'UTR + rab-3p::his-24::tagBFP::unc-54 3'UTR]. Keep plates covered to avoid unnecessary exposure to light. Pan-neuronal expression of ChR2 (H134R) along with Voltron volatage indicator and nuclear-localized tagBFP. Reporter construct contains an artificial intron (AI). Reference: Sharma AK, et al. Genetics 2024 May 11:iyae077. doi: 10.1093/genetics/iyae077 PMID: 38733622. Mutagen: N/A Outcrossed: x0 Made by: Anuj Sharma Received: 07/12/24 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML376 Species: C. elegans Genotype: juSi164 unc-119(ed3) III; wtfEx296. Description: juSi164 [mex-5p::HIS-72::miniSOG + Cbr-unc-119(+)] III. wtfEx296 [rab-3p::AI::gur-3G::unc-54 3'UTR + rab-3p::AI::prdx-2G::SL2::his-24::tagRFP::unc-54 3'UTR + rab-3p::his-24::GCaMP6s::unc-54 3'UTR]. Pick RFP+ to maintain. Keep plates covered to avoid unnecessary exposure to light. Worms expressing a purple light-sensitive optogenetic protein system: pan-neuronal expression of GUR-3 and PRDX-2 with nuclear-localized tagRFP and GCaMP6s. Reporter construct contains an artificial intron (AI). Reference: Sharma AK, et al. Genetics 2024 May 11:iyae077. doi: 10.1093/genetics/iyae077 PMID: 38733622. Mutagen: N/A Outcrossed: x0 Made by: Anuj Sharma Received: 07/12/24 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML405 Species: C. elegans Genotype: juSi164 unc-119(ed3) III; wtfEx315. Description: juSi164 [mex-5p::HIS-72::miniSOG + Cbr-unc-119(+)] III. wtfEx315 [5xQUAS::(delta)pes-10P::AI::gur-3G::unc-54 3'UTR + 5xQUAS::(delta)pes-10P::AI::prdx-2G::unc-54 3'UTR + rab-3p::AI::QF+hGR::unc-54 3'UTR + unc-122p::GFP]. Pick animals with GFP+ coelomocytes to maintain. Worms expressing a purple light-sensitive optogenetic protein system: pan-neuronal expression of GUR-3 and PRDX-2. Reporter construct contains an artificial intron (AI). Reference: Sharma AK, et al. Genetics 2024 May 11:iyae077. doi: 10.1093/genetics/iyae077 PMID: 38733622. Mutagen: N/A Outcrossed: x0 Made by: Anuj Sharma Received: 07/12/24 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML438 Species: C. elegans Genotype: juSi164 unc-119(ed3) III; wtfIs335. Description: juSi164 [mex-5p::HIS-72::miniSOG + Cbr-unc-119(+)] III. wtfIs335 [5xQUAS+(delta)pes-10p::AI::eTsChR::unc-54 3'UTR + rab-3p::AI::QF+hGR::SL2::tagBFP::unc-54 3'UTR + rab-3p::his-24::tagRFP::unc-54 3'UTR + rab-3p::his-24::GCaMP6s::unc-54 3'UTR]. Keep plates covered to avoid unnecessary exposure to light. Pan-neuronal eTsChR on activation using dex treatment via QF+hGR. Worms also express calcium sensing fluorescence protein- GCaMP6s in nuclei of each neurons. Reporter construct contains an artificial intron (AI). Reference: Sharma AK, et al. Genetics 2024 May 11:iyae077. doi: 10.1093/genetics/iyae077 PMID: 38733622. Mutagen: N/A Outcrossed: x0 Made by: Anuj Sharma Received: 07/12/24 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML456 Species: Caenorhabditis elegans Genotype: wtfIs348. Description: wtfIs348 [pAS3-5xQUAS::(delta)pes-10p::AI::gur-3G::unc-54 3' UTR + pAS3-5xQUAS::(delta)pes-10p::AI::prdx-2G::unc-54 3' UTR + pAS-3-rab-3p::AI::QF+GR::unc-54 3' UTR + unc-122::GFP]. Keep plates covered to avoid unnecessary exposure to light. This strain expresses a purple light-sensitive optogenetic protein system (i.e., GUR-3 and PRDX-2) in each neuron, and GFP in coelomocytes. Reference: Randi F, et al. Nature 2023 Nov;623(7986):406-414. doi: 10.1038/s41586-023-06683-4. PMID: 37914938. Sharma AK, et al. Genetics 2024 May 11:iyae077. doi: 10.1093/genetics/iyae077 PMID: 38733622. Mutagen: none Outcrossed: x8 Made by: Anuj Kumar Sharma Received: 08/24/22 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML462 Species: Caenorhabditis elegans Genotype: otIs669 V; wtfIs145; wtfIs348. Description: wtfIs145 [rab-3p::his-24::GCaMP6s::unc-54 3' UTR + pBX]. wtfIs348 [pAS3-5xQUAS::(delta)pes-10p::AI::gur-3G::unc-54 3' UTR + pAS3-5xQUAS::(delta)pes-10p::AI::prdx-2G::unc-54 3' UTR + pAS3-rab-3p::AI::QF+GR::unc-54 3' UTR + unc-122::GFP]. Keep plates covered to avoid unnecessary exposure to light. This strain expresses a purple light-sensitive optogenetic protein system (i.e., GUR-3 and PRDX-2) in each neuron, and GFP in coelomocytes. GCaMP6s transgene allows for imaging whole-brain calcium activity with neuronal identification using NeuroPAL system. See description of strain OH15262 for full description of otIs669 NeuroPAL (Neuronal Polychromatic Atlas of Landmarks) transgene (Yemini E, et al. Cell. 2021 Jan 7;184(1):272-288.e11. PMID: 33378642). Derived by out-crossing parental strain OH15262 an additional six times before incorporating other transgenes. References: Yu X, et al. Elife. 2021 Jul 14;10:e66410. doi: 10.7554/eLife.66410. PMID: 34259623. Randi F, et al. Nature 2023 Nov;623(7986):406-414. doi: 10.1038/s41586-023-06683-4. PMID: 37914938. Sharma AK, et al. Genetics 2024 May 11:iyae077. doi: 10.1093/genetics/iyae077 PMID: 38733622. Mutagen: none Outcrossed: x0 Made by: Anuj Kumar Sharma Received: 08/24/22 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML470 Species: Caenorhabditis elegans Genotype: juSi164 unc-119(ed3) III; wtfIs458. Description: juSi164 [mex-5p::HIS-72::miniSOG + Cbr-unc-119(+)] III. wtfIs458 [mec-4::Chrimson4.2::SL2::mCherry::unc-54 3' UTR + unc-122::GFP]. Maintain in the covered box to avoid unnecessary exposure to ambient light. Upon blue light treatment (460 nm LED light for 30 min at 4 Hz with 2 mW/mm2), Histone-miniSOG in the germline can induce heritable mutations. Transgenic animals express light-gated ion channel Chrimson and a fluorescent protein mCherry in mechanosensory neurons alongside GFP in coelomocytes. Reference: Liu M, et al. PLoS Biol. 2022 Jan 28;20(1):e3001524. doi: 10.1371/journal.pbio.3001524. PMID: 35089912. Mutagen: none Outcrossed: x0 Made by: Anuj Sharma Received: 08/11/21 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML496 Species: C.elegans Genotype: wtfIs465. Description: wtfIs465 [lim-4p::gtACR2::SL2::eGFP::unc-54 3' UTR + unc-122::RFP]. Transgenic animals express light-gated ion channel gtACR2 and a fluorescent protein eGFP in turning associated neurons RIV, SMB and SAA, alongside RFP in coelomocytes. Reference: Kumar S, et al. PLoS Biol. 2023 Sep 21;21(9):e3002280. doi: 10.1371/journal.pbio.3002280. PMID: 37733772. Mutagen: Outcrossed: x6 Made by: Anuj Sharma & Sandeep Kumar Received: 12/20/22 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML499 Species: C.elegans Genotype: wtfIs46; wtfIs465. Description: wtfIs46 [mec-4p::Chrimson::SL2::mCherry::unc-54 3'UTR]. wtfIs465 [lim-4p::gtACR2::SL2::eGFP::unc-54 3' UTR + unc-122::RFP]. Transgenic animals express light-gated ion channel Chrimson and mCherry in mechanosensory neurons, and light-gated ion channel gtACR2 and eGFP in turning associated neurons RIV, SMB and SAA. RFP expression in coelomocytes. Reference: Kumar S, et al. PLoS Biol. 2023 Sep 21;21(9):e3002280. doi: 10.1371/journal.pbio.3002280. PMID: 37733772. Mutagen: Outcrossed: x6 Made by: Anuj Sharma and Sandeep Kumar Received: 12/20/22 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML5 Species: Caenorhabditis elegans Genotype: otIs355 IV; lin-15B&lin-15A(n765) X; kyIs51. Description: otIs355 [rab-3p(prom1)::2xNLS::TagRFP] IV. kyIs51 [odr-2b::GFP + lin-15(+)]. Pan-neuronal nuclear RFP expression. Cytoplasmic GFP expressed in the odr-2b neurons including AIZ, AIB, SIAV, AVG, RIV, ASG and IL2 neurons. Derived from parental strains QW1155 and CX3300. Reference: Nguyen JP, et al. Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):E1074-81. Mutagen: Outcrossed: x Made by: George Plummer Received: 11/30/15 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML508 Species: Caenorhabditis elegans Genotype: unc-31(wtf502) IV; otIs669 V; wtfIs145; wtfIs348. Description: wtfIs145 [rab-3p::his-24::GCaMP6s::unc-54 3' UTR + pBX]. wtfIs348 [pAS3-5xQUAS::(delta)pes-10p::AI::gur-3G::unc-54 3' UTR + pAS3-5xQUAS::(delta)pes-10p::AI::prdx-2G::unc-54 3' UTR + pAS3-rab-3p::AI::QF+GR::unc-54 3' UTR + unc-122::GFP]. Keep plates covered to avoid unnecessary exposure to light. This strain expresses a purple light-sensitive optogenetic protein system (i.e., GUR-3 and PRDX-2) in each neuron, and GFP in coelomocytes. GCaMP6s transgene allows for imaging whole-brain calcium activity with neuronal identification using NeuroPAL system. unc-31(wtf502) is a CRISPR-engineered deletion allele. See description of strain OH15262 for full description of otIs669 NeuroPAL (Neuronal Polychromatic Atlas of Landmarks) transgene (Yemini E, et al. Cell. 2021 Jan 7;184(1):272-288.e11. PMID: 33378642). Derived by out-crossing parental strain OH15262 an additional six times before incorporating other transgenes. Reference: Randi F, et al. Nature 2023 Nov;623(7986):406-414. doi: 10.1038/s41586-023-06683-4. PMID: 37914938. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Anuj Kumar Sharma Received: 08/24/22 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML535 Species: C. elegans Genotype: wtfEx478. Description: wtfEx478 [rab-3p::AI::lite-1G::SL2::tagBFP::unc-54 3'UTR + unc-122p::GFP]. Pick GFP+ animals to maintain. Worms express purple/violet light-sensitive optogenetic protein LITE-1 pan-neuronallly and nuclear-localized blue fluorescent protein tagBFP via SL2 trans-slpicing regulatory sequence. Worms also express GFP in coelomocytes. Reporter construct contains an artificial intron (AI). Reference: Sharma AK, et al. Genetics 2024 May 11:iyae077. doi: 10.1093/genetics/iyae077 PMID: 38733622. Mutagen: N/A Outcrossed: x0 Made by: Anuj Sharma Received: 07/12/24 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML540 Species: C. elegans Genotype: juSi164 unc-119(ed3) III; wtfIs483. Description: juSi164 [mex-5p::HIS-72::miniSOG + Cbr-unc-119(+)] III. wtfIs483 [rab-3p::AI::lite-1G::unc-54 3'UTR + rab-3p::AI::prdx-2G::SL2::his-24::tagRFP::unc-54 3'UTR + rab-3p::his-24::GCaMP6s::unc-54 3'UTR]. Keep plates covered to avoid unnecessary exposure to light. Pan-neuronal expression of a purple/violet light-sensitive optogenetic protein system LITE-1 and PRDX-2 with nuclear-localized tagRFP and GCaMP6s. Reporter construct contains an artificial intron (AI). Reference: Sharma AK, et al. Genetics 2024 May 11:iyae077. doi: 10.1093/genetics/iyae077 PMID: 38733622. Mutagen: N/A Outcrossed: x0 Made by: Anuj Sharma Received: 07/12/24 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML546 Species: Caenorhabditis elegans Genotype: wtfEx496. Description: wtfEx496 [pAS3-rig-3p::AI::gur-3G::SL2::tagRFP::unc-54 3'UTR + pAS3-rig-3p::AI::prdx-2G::SL2::tagBFP::unc-54 3'UTR]. Pick animals either BFP or RFP expression in head neurons to maintain. Keep plates covered to avoid unnecessary exposure to light. This strain expresses a purple light-sensitive optogenetic protein system (i.e., GUR-3 and PRDX-2) alongside fluorescent proteins tagBFP and tagRFP in command interneurons AVA, and nearby pharyngeal neurons I1, I4, M4, NSM using rig-3 promoter. Worms exhibit higher reversal rate on exposing to blue light (peak value ~470) compared to controls. Reference: Randi F, et al. Nature 2023 Nov;623(7986):406-414. doi: 10.1038/s41586-023-06683-4. PMID: 37914938. Mutagen: No Outcrossed: x0 Made by: Anuj Sharma Received: 05/30/23 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML551 Species: Caenorhabditis elegans Genotype: gur-3(ok2245) X; wtfIs5. Description: wtfIs5 [rab-3p::NLS::GCaMP6s + rab-3p::NLS::tagRFP]. Integrated calcium indicator GCaMP6s and calcium-insensitive fluorescent protein RFP in the nuclei of all neurons in a gur-3(ok2245) mutant background. Derived from parental strain AML14 by integration of wtfEx4. Reference: Gauthey W, et al. Curr Biol. 2024 Jan 8;34(1):R14-R15. doi: 10.1016/j.cub.2023.10.043. PMID: 38194919. Mutagen: Outcrossed: x0 Made by: Anuj Sharma Received: 01/12/23 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML554 Species: Caenorhabditis elegans Genotype: lite-1(ce314) gur-3(ok2245) X; wtfIs5. Description: wtfIs5 [rab-3p::NLS::GCaMP6s + rab-3p::NLS::tagRFP]. Integrated calcium indicator GCaMP6s and calcium-insensitive fluorescent protein RFP in the nuclei of all neurons in a lite-1(ce314) gur-3(ok2245) double mutant background. Derived from parental strain AML14 by integration of wtfEx4. Reference: Gauthey W, et al. Curr Biol. 2024 Jan 8;34(1):R14-R15. doi: 10.1016/j.cub.2023.10.043. PMID: 38194919. Mutagen: Outcrossed: x0 Made by: Anuj Sharma Received: 01/12/23 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML580 Species: C. elegans Genotype: wtfIs491. Description: wtfIs491 [inx-1p::twk-18(gf)::mCherry + unc-122p::RFP]. AIB(-) activated potassium channel. Expression of twk-18 gain-of-function mutant in AIB neurons causes permanent inhibition. RFP expression in coelomocytes. Reference: Chen KS, et al. Olfactory learning alters navigation strategies and behavioral variability in C. elegans. ArXiv, Feb 23:arXiv:2311.07117v2. PMID: 38013890. Mutagen: N/A Outcrossed: x6 Made by: Anuj Sharma & Kevin Chen Received: 07/12/24 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML597 Species: C. elegans Genotype: lgc-47(sy1501) X; wtfIs46. Description: wtfIs46 [mec-4p::Chrimson::SL2::mCherry::unc-54 3'UTR]. Expression of activating opsin molecule Chrimson in six gentle-touch mechanosensory neurons (ALML/R, AVM, PLML/R, PVM). Reference: Kumar S, et al. An inhibitory acetylcholine receptor gates context dependent mechanosensory processing in C. elegans. bioRxiv 2024.03.21.586204; doi: https://doi.org/10.1101/2024.03.21.586204. PMID: 38585821. Mutagen: N/A Outcrossed: x0 Made by: Anuj Sharma and Sandeep Kumar Received: 07/12/24 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML614 Species: C. elegans Genotype: lgc-47(sy1501) X; wtfIs46; wtfEx535. Description: wtfIs46 [mec-4p::Chrimson::SL2::mCherry::unc-54 3'UTR]. wtfEx535 [tdc-1p::AI::lgc- 47::SL2::his-24::tagRFP + unc-122p::GFP]. Pick animals with GFP+ coelomocytes to maintain. Expression of activating opsin molecule Chrimson in six gentle-touch mechanosensory neurons (ALML/R, AVM, PLML/R, PVM). Rescuing LGC-47 expression in RIML/R neurons. Reporter construct contains an artificial intron (AI). Reference: Kumar S, et al. An inhibitory acetylcholine receptor gates context dependent mechanosensory processing in C. elegans. bioRxiv 2024.03.21.586204; doi: https://doi.org/10.1101/2024.03.21.586204. PMID: 38585821. Mutagen: N/A Outcrossed: x0 Made by: Anuj Sharma Received: 07/12/24 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML617 Species: C. elegans Genotype: lgc-47(sy1501) X; wtfIs46; wtfEx538. Description: wtfIs46 [mec-4p::Chrimson::SL2::mCherry::unc-54 3'UTR]. wtfEx538 [npr-9p::AI::lgc-47::SL2::tagBFP + unc-122p::GFP]. Pick animals with GFP+ coelomocytes to maintain. Expression of activating opsin molecule Chrimson in six gentle-touch mechanosensory neurons (ALML/R, AVM, PLML/R, PVM). Rescuing LGC-47 expression in AIB neuron. Reporter construct contains an artificial intron (AI). Reference: Kumar S, et al. An inhibitory acetylcholine receptor gates context dependent mechanosensory processing in C. elegans. bioRxiv 2024.03.21.586204; doi: https://doi.org/10.1101/2024.03.21.586204. PMID: 38585821. Mutagen: N/A Outcrossed: x0 Made by: Anuj Sharma Received: 07/12/24 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML618 Species: C. elegans Genotype: lgc-47(sy1501) X; wtfIs46; wtfEx539. Description: wtfIs46 [mec-4p::Chrimson::SL2::mCherry::unc-54 3'UTR]. wtfEx539 [rig-3p::AI::lgc-47::SL2::GFP + unc-122p::GFP]. Pick animals with GFP+ coelomocytes to maintain. Expression of activating opsin molecule Chrimson in six gentle-touch mechanosensory neurons (ALML/R, AVM, PLML/R, PVM). Rescuing LGC-47 expression in AVA neuron. Reporter construct contains an artificial intron (AI). Reference: Kumar S, et al. An inhibitory acetylcholine receptor gates context dependent mechanosensory processing in C. elegans. bioRxiv 2024.03.21.586204; doi: https://doi.org/10.1101/2024.03.21.586204. PMID: 38585821. Mutagen: N/A Outcrossed: x0 Made by: Anuj Sharma Received: 07/12/24 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML622 Species: C. elegans Genotype: lgc-47(sy1501) X; wtfIs46; wtfEx543. Description: wtfIs46 [mec-4p::Chrimson::SL2::mCherry::unc-54 3'UTR]. wtfEx543 [tdc-1p::AI::lgc-47::SL2::his-24::tagRFP + npr-9p::AI::lgc-47::SL2::tagBFP + rig-3p::AI::lgc-47::SL2::GFP + unc-122p::GFP]. Pick animals with GFP+ coelomocytes to maintain. Expression of activating opsin molecule Chrimson in six gentle-touch mechanosensory neurons (ALML/R, AVM, PLML/R, PVM). Rescuing LGC-47 expression in AIB, AVA, and RIM neurons. Reporter construct contains an artificial intron (AI). Reference: Kumar S, et al. An inhibitory acetylcholine receptor gates context dependent mechanosensory processing in C. elegans. bioRxiv 2024.03.21.586204; doi: https://doi.org/10.1101/2024.03.21.586204. PMID: 38585821. Mutagen: N/A Outcrossed: x0 Made by: Anuj Sharma Received: 07/12/24 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML627 Species: C. elegans Genotype: acc-1 (tm3268) IV; wtfIs46. Description: wtfIs46 [mec-4p::Chrimson::SL2::mCherry::unc-54 3'UTR]. Expression of activating opsin molecule Chrimson in six gentle-touch mechanosensory neurons (ALML/R, AVM, PLML/R, PVM). Reference: Kumar S, et al. An inhibitory acetylcholine receptor gates context dependent mechanosensory processing in C. elegans. bioRxiv 2024.03.21.586204; doi: https://doi.org/10.1101/2024.03.21.586204. PMID: 38585821. Mutagen: N/A Outcrossed: x0 Made by: Anuj Sharma Received: 07/12/24 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML659 Species: Caenorhabditis elegans Genotype: acc-1(tm3268) IV; lgc-47(sy1501) X; wtfIs46. Description: wtfIs46 [mec-4p::Chrimson::SL2::mCherry::unc-54 3'UTR]. mec-4 promoter drives expression of activating opsin molecule Chrimson and fluorescent protein mCherry in six gentle-touch mechanosensory neurons (ALML/R, AVM, PLML/R, PVM). Reference: Kumar S, et al. An inhibitory acetylcholine receptor gates context dependent mechanosensory processing in C. elegans. (2024) iScience. https://www.sciencedirect.com/science/article/pii/S2589004224020017 PMID: 38585821. Mutagen: none Outcrossed: x0 Made by: Anuj Sharma Received: 09/27/24 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML67 Species: Caenorhabditis elegans Genotype: wtfIs46. Description: wtfIs46 [mec-4p::Chrimson::SL2::mCherry::unc-54 3'UTR]. Transgenic animals express light-gated ion channel Chrimson and mCherry in mechanosensory neurons. Reference: Liu M, et al. eLife 2018;7:e36419 DOI: 10.7554/eLife.36419. Mutagen: Outcrossed: x6 Made by: Anuj Kumar Sharma Received: 03/26/18 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML70 Species: Caenorhabditis elegans Genotype: lite-1(ce314) X; wtfIs5. Description: wtfIs5 [rab-3p::NLS::GCaMP6s + rab-3p::NLS::tagRFP]. Integrated calcium indicator GCaMP6s and calcium-insensitive fluorescent protein RFP in the nuclei of all neurons in a lite-1(ce314) mutant background. Derived from parental strain AML14 by integration of wtfEx4. Reference: Gauthey W, et al. Curr Biol. 2024 Jan 8;34(1):R14-R15. doi: 10.1016/j.cub.2023.10.043. PMID: 38194919. Mutagen: Outcrossed: x0 Made by: Anuj Sharma Received: 10/22/18 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AML73 Species: C. elegans Genotype: pha-1(e2123)III; wtfEx48. Description: wtfEx48 [rab-3p::Chrimson::unc-54 3'UTR + pha-1(+)]. Maintain at 20-25C to select for animals carrying the array. Keep plates covered to avoid unnecessary exposure to light. Pan-neuronal expression of Chrimson. Reference: Sharma AK, et al. Genetics 2024 May 11:iyae077. doi: 10.1093/genetics/iyae077 PMID: 38733622. Mutagen: N/A Outcrossed: x0 Made by: Anuj Sharma Received: 07/12/24 Princeton University, Princeton NJ ----------------------------------------------------------------------------- Strain: AMP100 Species: C. elegans Genotype: ieSi57 II; rpb-2(cer135[rpb-2::GFP(delta)piRNA::AID::3xFLAG]) III. Description: ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. cer135 is a rpb-2::GFP(delta)piRNA::AID::3xFLAG tag inserted into the endogenous rpb-2 locus. This strain allows auxin-dependent disruption of RNA polymerase II with dose-dependent lifespan shortening. Reference: Oswal N, et al. PLoS Comput Biol. 2022 Sep 30;18(9):e1010415. PMID: 36178967. Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: ‪J. Vicencio, N. Oswal, N.Stroustrup & J. Ceron Received: 12/21/22 Centre de Regulacio Genomica, Barcelona, Spain ----------------------------------------------------------------------------- Strain: AMP101 Species: C. elegans Genotype: weSi174 II; daf-2(syb1177[daf-2::AID::TEV::3xFLAG]) III. Description: weSi174 [eif-3.Bp::TIR-1::linker::mCherry(dpiRNA)::tbb-2 3'UTR + unc-119(+)] II. Auxin-inducible degradation of DAF-2::AID causes dauer formation. Reference: Eder M, et al. Cell. 2024 Jul 25;187(15):3919-3935.e19. doi: 10.1016/j.cell.2024.05.050. PMID: 38908368. Mutagen: Outcrossed: x1 Made by: Natasha Oswal Received: 12/30/24 Centre de Regulacio Genomica, Barcelona, Spain ----------------------------------------------------------------------------- Strain: AMP116 Species: C. elegans Genotype: weSi174 II; daf-2(syb1177[daf-2::AID::TEV::3xFLAG]) glp-1(e2141) III. Description: weSi174 [eif-3.Bp::TIR-1::linker::mCherry(dpiRNA)::tbb-2 3'UTR + unc-119(+)] II. Maintain at 20C or less. Sterile at 25C. Auxin-inducible degradation of DAF-2::AID causes dauer formation. Reference: Eder M, et al. Cell. 2024 Jul 25;187(15):3919-3935.e19. doi: 10.1016/j.cell.2024.05.050. PMID: 38908368. Mutagen: Outcrossed: x1 Made by: Natasha Oswal Received: 12/30/24 Centre de Regulacio Genomica, Barcelona, Spain ----------------------------------------------------------------------------- Strain: AN170 Species: Caenorhabditis elegans Genotype: aff-1(ty4) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT (heterozygotes), Unc-4 worms which are strong Egl (ty4 homozygotes), and paralyzed Dpy Uncs (mnC1 homozygotes). Mutagen: EMS Outcrossed: x5 Made by: J Choi/A Newman Received: 02/19/08 Wright State University ----------------------------------------------------------------------------- Strain: AN87 Species: Caenorhabditis elegans Genotype: sel-12(ty11) X. Description: Egl. Premature stop codon. Mutagen: EMS Outcrossed: x6 Made by: Nese Cinar Received: 12/04/01 Baylor College of Medicine, Houston, TX ----------------------------------------------------------------------------- Strain: ANA65 Species: C. elegans Genotype: adeIs1 II; unc-119(ed3) III. Description: adeIs1 [mex-5::spd-1::GFP + unc-119(+)] II. The transgene has been inserted on chromosome II, using the MosSCI technique. This strains expresses the SPD-1 protein fused to GFP in the germline (both males and females) and in embryos. SPD-1 is nucleolar in interphase and labels the central spindle in mitosis and meiosis, later accumulating at the midbody. Reference: Nahaboo W, et al. Mol Biol Cell. 2015 Jun 1;26(11):2020-9. doi: 10.1091/mbc.E14-12-1577. Mutagen: MosSCI Outcrossed: x2 Made by: Melissa ZOUAK Received: 11/12/19 Ecole Normale Superieure de Lyon, Lyon, France ----------------------------------------------------------------------------- Strain: ANA72 Species: C. elegans Genotype: adeIs1 II; unc-119(ed3) III; ltIs37 IV. Description: adeIs1 [mex-5::spd-1::GFP + unc-119(+)] II. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Superficially wild-type. SPD-1::GFP and mCherry-tagged histones allow visualisation of chromatin with central spindle/midbody during cell divisions. Reference: Nahaboo W, et al. Mol Biol Cell. 2015 Jun 1;26(11):2020-9. Mutagen: MosSCI Outcrossed: x2 Made by: Melissa ZOUAK Received: 11/12/19 Ecole Normale Superieure de Lyon, Lyon, France ----------------------------------------------------------------------------- Strain: ANR149 Species: Caenorhabditis elegans Genotype: rrf-1(pk1417) I; pkIs2386. Description: pkIs2386 [unc-54p::alphasynuclein::YFP + unc-119(+)]. YFP expression in the muscles. Derived by crossing parental strains NL5901 and MAH23 to produce a Parkinson's model with germline-only RNAi. unc-119 might still be present in the background, but likely lost during crossing. Reference: Snow S, et al. (2024). Neuronal CBP-1 is required for enhanced body muscle proteostasis in response to reduced translation downstream of mTOR. Frontiers in Bioscience-Landmark. Mutagen: none Outcrossed: x0 Made by: Santina Snow Received: 04/15/24 Mt Desert Island Bio Lab, Salisbury Cove, ME ----------------------------------------------------------------------------- Strain: ANR153 Species: Caenorhabditis elegans Genotype: rde-1(ne300) V.; neIs9 X; pkIs2386. Description: pkIs2386 [unc-54p::alphasynuclein::YFP + unc-119(+)]. neIs9 [myo-3::HA::rde-1 + rol-6(su1006)] X. Rollers. YFP expression in the muscles. Derived by crossing parental strains NL5901 and WM118 to produce a Parkinson's model with muscle-only RNAi. Reference: Snow S, et al. (2024). Neuronal CBP-1 is required for enhanced body muscle proteostasis in response to reduced translation downstream of mTOR. Frontiers in Bioscience-Landmark. Mutagen: none Outcrossed: x0 Made by: Santina Snow Received: 04/15/24 Mt Desert Island Bio Lab, Salisbury Cove, ME ----------------------------------------------------------------------------- Strain: ANR165 Species: C. elegans Genotype: eif-2alpha(rog3) I. Description: eif-2alpha(rog3) is a (S49A) substitution allele (I:2073439..2073448; WS220) removing P site and PAM site. eif-2alpha (Y37E3.10) encodes the alpha subunit of eukaryotic translation initiation factor 2 (eIF2). Reference: Rollins J, et al. Loss of eif-2alpha phosphorylation on S49 (mammalian S51) associated with the integrated stress response hastens development in C. elegans. MicroPubl Biol. 2017;2017:10.17912/W2BM1S. doi: 10.17912/W2BM1S. Erratum in: MicroPubl Biol. 2020 Feb 27;2020: PMID: 32292896. Mutagen: Outcrossed: x0 Made by: Jarod Rollins Received: 10/23/23 Mt Desert Island Bio Lab, Salisbury Cove, ME ----------------------------------------------------------------------------- Strain: ANR168 Species: Caenorhabditis elegans Genotype: lin-15B(n744) X; pkIs2386; uIs57. Description: pkIs2386 [unc-54p::alphasynuclein::YFP + unc-119(+)]. uIs57 [unc-119p::YFP + unc-119p::sid-1 + mec-6p::mec-6]. YFP expression in the muscles. Parkinson's model with hypersensitive neuronal RNAi. Reference: Snow S, et al. (2024). Neuronal CBP-1 is required for enhanced body muscle proteostasis in response to reduced translation downstream of mTOR. Frontiers in Bioscience-Landmark. Mutagen: none Outcrossed: x0 Made by: Santina Snow Received: 04/15/24 Mt Desert Island Bio Lab, Salisbury Cove, ME ----------------------------------------------------------------------------- Strain: AP36 Species: Caenorhabditis elegans Genotype: mep-1(ok421)/nT1 [qIs51] (IV;V). Description: qIs51 [myo-2p::GFP + pes-10p::GFP + F22B7.9p::GFP]. Heterozygotes are wild-type GFP+ and segregate non-GFP ok421 homozygotes, wild-type GFP+ heterozygotes, and arrested nT1[qIs51] aneuploids. Pick wild-type GFP+ and check for correct segregation of progeny to maintain. Received new stock 12/02. Mutagen: UV/TMP Outcrossed: x5 Made by: A Puoti Received: 08/14/02 University of Fribourg, Switzerland ----------------------------------------------------------------------------- Strain: APL31 Species: C. elegans Genotype: lin-12(ljf31[lin-12::mNeonGreen[C1]::3xFLAG]) III. Description: mNG and 3xFLAG tags fused to the C-terminal end of the intracellular domain of the endogenous lin-12 locus using a 9 amino acid flexible linker. Reference: Pani AM, et al. A new toolkit to visualize and perturb endogenous LIN-12/Notch signaling in C. elegans. MicroPubl Biol. 2022 Jul 28;2022:10.17912/micropub.biology.000603. doi: 10.17912/micropub.biology.000603. PMID: 35966394. Mutagen: Outcrossed: x0 Made by: Ariel Pani Received: 10/23/23 University of Virginia ----------------------------------------------------------------------------- Strain: AQ3236 Species: C. elegans Genotype: ljSi2 II; unc-119(ed3) III. Description: ljSi2 [mec-7::GCaMP6m::SL2::TagRFP + unc-119(+)] II. GCaMP6m (13.693) and RFP expressed in touch receptor neurons (ALML/R, AVM, PVM, PLML/R). Dual expression of GCamp6m and RFP allows for ratio-metric corrections of motion artifacts. Reference: Cho Y, et al. Lab Chip. 2017 Jul 25;17(15):2609-2618. Mutagen: Outcrossed: x0 Made by: Dr. William Schafer Received: 11/10/17 Georgia Institute of Technology ----------------------------------------------------------------------------- Strain: AQ351 Species: C. elegans Genotype: bus-8A(lj22) X. Description: Skiddy, bleach-sensitive, drug-sensitive, Bus, resistant to Leucobacter Verde2, hypersensitive to Leucobacter Verde1. lj22 is a missense mutation (R32C) in bus-8A and might also affect bus-8B (out-of-frame 5'exon U1). Reference: Partridge et al. (2008) PMID: 18395708. Mutagen: EMS Outcrossed: x2? Made by: A W Tearle Received: 10/10/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: AQ866 Species: Caenorhabditis elegans Genotype: ser-4(ok512) III. Description: Y22D7AR.13. Hyperactive 5HT induced egglaying. Homozygous. Outer Left Sequence: AATTATCGGATTTAGGGCCG. Outer Right Sequence: ATGGAACGGAGCATTATTCG. Inner Left Sequence: CAACACGCAACGAATGTACC. Inner Right Sequence: TGTGAAGTTTGGGAGGCTTT. Inner primer WT PCR product: 3126. Outcrossed 5 times by Stanley Shyn. URL: http://www.celeganskoconsortium.omrf.org. Mutagen: UV/TMP Outcrossed: x5 Made by: Stanley Shyn Received: 02/24/03 MRC - LMB, Cambridge, UK ----------------------------------------------------------------------------- Strain: AR1 Species: Caenorhabditis elegans Genotype: hcp-6(mr17) I. Description: 15C: no phenotype. 26C: shifted as L4 or later results in 100% embryonic lethality; shifted before L4 results in Unc and Sterile animals. Intermediate phenotypes between 20-23C. Mutagen: EMS Outcrossed: x5 Made by: Roth/Morrison/Stear Received: 11/04/02 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR10 Species: Caenorhabditis elegans Genotype: cysl-1(mr25) X. Description: Sensitive to hydrogen sulfide and hydrogen cyanide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32. Mutagen: EMS Outcrossed: x1 Made by: Mark Budde Received: 09/10/11 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR11 Species: Caenorhabditis elegans Genotype: cysl-1(mr26) X. Description: Sensitive to hydrogen sulfide and hydrogen cyanide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32. Mutagen: EMS Outcrossed: x1 Made by: Mark Budde Received: 09/10/11 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR12 Species: Caenorhabditis elegans Genotype: suls-1(mr27) V. Description: Sensitive to hydrogen sulfide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32. Mutagen: EMS Outcrossed: x0 Made by: Mark Budde Received: 09/10/11 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR13 Species: Caenorhabditis elegans Genotype: sqrd-1(mr28) IV. Description: Sensitive to hydrogen sulfide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32. Mutagen: EMS Outcrossed: x0 Made by: Mark Budde Received: 09/10/11 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR14 Species: Caenorhabditis elegans Genotype: cysl-1(mr29) X. Description: Sensitive to hydrogen sulfide and hydrogen cyanide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32. Mutagen: EMS Outcrossed: x0 Made by: Mark Budde Received: 09/10/11 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR15 Species: Caenorhabditis elegans Genotype: sqrd-1(mr30) IV. Description: Sensitive to hydrogen sulfide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32. Mutagen: EMS Outcrossed: x0 Made by: Mark Budde Received: 09/10/11 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR16 Species: Caenorhabditis elegans Genotype: suls-2(mr31) I. Description: Sensitive to hydrogen sulfide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32. Mutagen: EMS Outcrossed: x0 Made by: Mark Budde Received: 09/10/11 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR17 Species: Caenorhabditis elegans Genotype: sqrd-1(mr32) IV. Description: Sensitive to hydrogen sulfide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32. Mutagen: EMS Outcrossed: x0 Made by: Mark Budde Received: 09/10/11 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR18 Species: Caenorhabditis elegans Genotype: cysl-1(mr33) X. Description: Sensitive to hydrogen sulfide and hydrogen cyanide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32. Mutagen: EMS Outcrossed: x0 Made by: Mark Budde Received: 09/10/11 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR19 Species: Caenorhabditis elegans Genotype: cysl-1(mr34) X. Description: Sensitive to hydrogen sulfide and hydrogen cyanide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32. Mutagen: EMS Outcrossed: x1 Made by: Mark Budde Received: 09/10/11 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR2 Species: Caenorhabditis elegans Genotype: hcp-6(mr17) unc-73(e936) I. Description: Unc at all temperatures. For hcp-6: 15C: no phenotype. 26C: shifted as L4 or later results in 100% embryonic lethality; shifted before L4 results in Unc and Sterile animals. Intermediate phenotypes between 20-23C. Mutagen: Outcrossed: x Made by: Jeff Stear Received: 11/04/02 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR20 Species: Caenorhabditis elegans Genotype: suls-1(mr35) V. Description: Sensitive to hydrogen sulfide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32. Mutagen: EMS Outcrossed: x0 Made by: Mark Budde Received: 09/10/11 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR21 Species: Caenorhabditis elegans Genotype: suls-2(mr36) I. Description: Sensitive to hydrogen sulfide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32. Mutagen: EMS Outcrossed: x1 Made by: Mark Budde Received: 09/10/11 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR23 Species: Caenorhabditis elegans Genotype: suls-1(mr38) V. Description: Sensitive to hydrogen sulfide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32. Mutagen: EMS Outcrossed: x0 Made by: Mark Budde Received: 09/10/11 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR24 Species: Caenorhabditis elegans Genotype: cysl-1(mr39) X. Description: Sensitive to hydrogen sulfide and hydrogen cyanide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32. Mutagen: EMS Outcrossed: x0 Made by: Mark Budde Received: 09/10/11 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR25 Species: Caenorhabditis elegans Genotype: cysl-1(mr40) X. Description: Sensitive to hydrogen sulfide and hydrogen cyanide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32. Mutagen: EMS Outcrossed: x0 Made by: Mark Budde Received: 09/10/11 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR3 Species: Caenorhabditis elegans Genotype: suls-1(mr18) V. Description: Sensitive to hydrogen sulfide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32. Mutagen: EMS Outcrossed: x0 Made by: Mark Budde Received: 09/10/11 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR4 Species: Caenorhabditis elegans Genotype: cysl-1(mr19) X. Description: Sensitive to hydrogen sulfide and hydrogen cyanide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32. Mutagen: EMS Outcrossed: x0 Made by: Mark Budde Received: 09/10/11 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR5 Species: Caenorhabditis elegans Genotype: hif-1(mr20) V. Description: Sensitive to hydrogen sulfide and hydrogen cyanide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32. Mutagen: EMS Outcrossed: x0 Made by: Mark Budde Received: 09/10/11 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR6 Species: Caenorhabditis elegans Genotype: sqrd-1(mr21) IV. Description: Sensitive to hydrogen sulfide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32. Mutagen: EMS Outcrossed: x0 Made by: Mark Budde Received: 09/10/11 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR7 Species: Caenorhabditis elegans Genotype: hif-1(mr22) V. Description: Sensitive to hydrogen sulfide and hydrogen cyanide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32. Mutagen: EMS Outcrossed: x0 Made by: Mark Budde Received: 09/10/11 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR8 Species: Caenorhabditis elegans Genotype: cysl-1(mr23) X. Description: Sensitive to hydrogen sulfide and hydrogen cyanide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32. Mutagen: EMS Outcrossed: x0 Made by: Mark Budde Received: 09/10/11 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: AR9 Species: Caenorhabditis elegans Genotype: sqrd-1(mr24) IV. Description: Sensitive to hydrogen sulfide. Reference: Budde MW, Roth MB. Genetics. 2011 Oct;189(2):521-32. Mutagen: EMS Outcrossed: x0 Made by: Mark Budde Received: 09/10/11 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: ARK7 Species: C. elegans Genotype: spe-36(nwk1[spe-36::gfp]) IV; him-5(e1490) V. Description: GFP tag inserted into endogenous spe-36 (F40F11.4) locus. SPE-36::GFP expression in spermatids and spermatozoa. Reference: Krauchunas AR, et al. Curr Biol. 2023 Jul 24;33(14):3056-3064.e5. doi: 10.1016/j.cub.2023.06.051. PMID: 37453426. Mutagen: Outcrossed: x0 Made by: Amber Krauchunas Received: 02/01/24 University of Delaware ----------------------------------------------------------------------------- Strain: ARM101 Species: Caenorhabditis elegans Genotype: wamSi101 V; unc-119(ed3) III. Description: wamSi101 [eft-3p::mTFP::unc-54 3'UTR + Cbr-unc-119(+)] V. Expresses a single copy of mTFP from eft-3 promoter; construct utilizes the unc-54 terminator and 3'UTR. Can be used as a standard for multicolor imaging and quantitative microscopy. Reference: Sands B, et al. 2018. Translational Medicine of Aging Volume 2, January 2018, Pages 1–10. Mutagen: Outcrossed: x0 Made by: Alex Mendenhall Received: 05/04/18 University of Washington ----------------------------------------------------------------------------- Strain: ARM103 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wamSi103 V. Description: wamSi103 [eft-3p::mKO2::unc-54 3'UTR + Cbr-unc-119(+)] V. Expresses a single copy of mKO2 from eft-3 promoter; construct utilizes the unc-54 terminator and 3'UTR. Can be used as a standard for multicolor imaging and quantitative microscopy. Reference: Sands B, et al. 2018. Translational Medicine of Aging Volume 2, January 2018, Pages 1–10. Mutagen: Outcrossed: x0 Made by: Alex Mendenhall Received: 05/04/18 University of Washington ----------------------------------------------------------------------------- Strain: ARM112 Species: Caenorhabditis elegans Genotype: wamSi112 II; unc-119(ed3) III Description: wamSi112 [eft-3p::mScarlet::unc-54 3'UTR + Cbr-unc-119(+)] II. Expresses a single copy of mScarlet from eft-3 promoter; construct utilizes the unc-54 terminator and 3'UTR. Can be used as a standard for multicolor imaging and quantitative microscopy. Reference: Sands B, et al. 2018. Translational Medicine of Aging Volume 2, January 2018, Pages 1–10. Mutagen: Outcrossed: x0 Made by: Alex Mendenhall Received: 05/04/18 University of Washington ----------------------------------------------------------------------------- Strain: ARM118 Species: Caenorhabditis elegans Genotype: wamSi118 II; unc-119(ed3) III. Description: wamSi118 [eft-3p::mCerulean3::unc-54 3'UTR + Cbr-unc-119(+)] II. Expresses a single copy of mCerulean3 from eft-3 promoter; construct utilizes the unc-54 terminator and 3'UTR. Can be used as a standard for multicolor imaging and quantitative microscopy. Reference: Sands B, et al. 2018. Translational Medicine of Aging Volume 2, January 2018, Pages 1–10. Mutagen: Outcrossed: x0 Made by: Alex Mendenhall Received: 05/04/18 University of Washington ----------------------------------------------------------------------------- Strain: ARM123 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wamSi123 V. Description: wamSi123 [eft-3p::mECitrine::unc-54 3'UTR + Cbr-unc-119 (+)] V. Expresses a single copy of mECitrine from eft-3 promoter; construct utilizes the unc-54 terminator and 3'UTR. Can be used as a standard for multicolor imaging and quantitative microscopy. Reference: Sands B, et al. 2018. Translational Medicine of Aging Volume 2, January 2018, Pages 1–10. Mutagen: Outcrossed: x0 Made by: Alex Mendenhall Received: 05/04/18 University of Washington ----------------------------------------------------------------------------- Strain: ARM3 Species: Caenorhabditis elegans Genotype: wamSi3 II; unc-119(ed3) III. Description: wamSi3 [eft-3p::mNeptune::unc-54 3'UTR + Cbr-unc-119(+)] II. Expresses a single copy of mNeptune from eft-3 promoter; construct utilizes the unc-54 terminator and 3'UTR. Can be used as a standard for multicolor imaging and quantitative microscopy. Reference: Sands B, et al. 2018. Translational Medicine of Aging Volume 2, January 2018, Pages 1–10. Mutagen: Outcrossed: x0 Made by: Alex Mendenhall Received: 05/04/18 University of Washington ----------------------------------------------------------------------------- Strain: ARM6 Species: Caenorhabditis elegans Genotype: wamSi6 II; unc-119(ed3) III. Description: wamSi6 [eft-3p::mTagBFP2::unc-54 3'UTR + Cbr-unc-119(+)] II. Expresses a single copy of mTagBFP2 from eft-3 promoter; construct utilizes the unc-54 terminator and 3'UTR. Can be used as a standard for multicolor imaging and quantitative microscopy. Reference: Sands B, et al. 2018. Translational Medicine of Aging Volume 2, January 2018, Pages 1–10. Mutagen: Outcrossed: x0 Made by: Alex Mendenhall Received: 05/04/18 University of Washington ----------------------------------------------------------------------------- Strain: ARM7 Species: Caenorhabditis elegans Genotype: wamSi7 II; unc-119(ed3) III. Description: wamSi7 [eft-3p::mTagRFP-T::unc-54 3'UTR + Cbr-unc-119(+)] II. Expresses a single copy of mTagRFP-T from eft-3 promoter; construct utilizes the unc-54 terminator and 3'UTR. Can be used as a standard for multicolor imaging and quantitative microscopy. Reference: Sands B, et al. 2018. Translational Medicine of Aging Volume 2, January 2018, Pages 1–10. Mutagen: Outcrossed: x0 Made by: Alex Mendenhall Received: 05/04/18 University of Washington ----------------------------------------------------------------------------- Strain: AS270 Species: Caenorhabditis elegans Genotype: gly-12(id47) X. Description: Phenotypically WT. F3.2 See also WBPaper00005927. Mutagen: UV/TMP Outcrossed: x5 Made by: Received: 03/12/03 Hospital for Sick Children, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: AS271 Species: Caenorhabditis elegans Genotype: gly-14(id48) III. Description: Phenotypically WT. M8ns. See also WBPaper00005927. Mutagen: UV/TMP Outcrossed: x5 Made by: Received: 03/12/03 Hospital for Sick Children, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: AS338 Species: Caenorhabditis elegans Genotype: dpy-6(e14) gly-13(ok712) X. Description: Mutagen: UV/TMP Outcrossed: x5 Made by: Harry Schachter Received: 01/24/04 Hospital for Sick Children, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: AT10 Species: Caenorhabditis elegans Genotype: srf-3(yj10) IV. Description: Superficially wild-type. Antibody staining required to observe phenotype. Contains at least one extraneous mutation in the background (Eur. Worm Mtg 2006, Venuz et al.). Mutagen: Outcrossed: x Made by: Received: 06/18/90 Worcester Polytechnic Institute ----------------------------------------------------------------------------- Strain: AT18 Species: C elegans Genotype: srf-6(yj13) II. Description: No visible phenotype. Whole genome sequenced strain. Reference: Grenache DG, et al. Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12388-93. Mutagen: EMS Outcrossed: x2 Made by: Karl J. Chin Received: 06/26/19 Worcester Polytechnic Institute ----------------------------------------------------------------------------- Strain: AT24 Species: C. elegans Genotype: srf-6(yj15) II. Description: No visible phenotype. Whole genome sequenced strain. Mutagen: EMS Outcrossed: x2 Made by: Karl J. Chin Received: 06/26/19 Worcester Polytechnic Institute ----------------------------------------------------------------------------- Strain: AT25 Species: C elegans Genotype: srf-6(yj41) II. Description: No visible phenotype. Whole genome sequenced strain. Mutagen: EMS Outcrossed: x2 Made by: Karl J. Chin Received: 06/27/19 Worcester Polytechnic Institute ----------------------------------------------------------------------------- Strain: AT28 Species: C. elegans Genotype: kyIs140 I; srf-6(yj13) unc-4(e120) II. Description: kyIs140 [str-2::GFP + lin-15(+)] I. Kinker; can't back up. srf-6 mutants express str-2::GFP in both AWC neurons (2AWC ON phenotype; wild-type phenotype is 1AWC ON): check for this phenotype to avoid reversion of srf-6(yj13). srf-6 mutants were originally identified by binding of an L1-specific antibody in later larval stages (L1-L4). Mutagen: EMS Outcrossed: x0 Made by: Samuel M Politz Received: 06/26/19 Worcester Polytechnic Institute ----------------------------------------------------------------------------- Strain: AT30 Species: C. elegans Genotype: kyIs140 I; nsy-1(ok593) unc-4(e120) II. Description: kyIs140 [str-2::GFP + lin-15(+)] I. nsy-1(ky593) has no visible phenotype, but can be tracked by linked Unc-4 phenotype (Kinker, can't back up). str-2::GFP is expressed in both AWC neurons. Mutagen: Outcrossed: x0 Made by: Samuel M Politz Received: 06/26/19 Worcester Polytechnic Institute ----------------------------------------------------------------------------- Strain: AT6 Species: Caenorhabditis elegans Genotype: srf-2(yj262) I. Description: Superficially wild-type. Antibody staining required to observe phenotype. Mutagen: EMS Outcrossed: x Made by: Miguel Estevez Received: 06/18/90 Worcester Polytechnic Institute ----------------------------------------------------------------------------- Strain: ATD1 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; sqt-3(sc8) par-1(b274) V/nT1[unc-?(n754) let-?] (IV;V); zuIs45 V. Description: zuIs45 [nmy-2p::nmy-2::GFP + unc-119(+)] V. Balanced heterozygotes are Unc and segregate Unc (heterozygotes), Rol Par (sqt-3 par-1 homozygotes; maternal effect lethal), and dead eggs (nT1 homozygotes). NMY-2::GFP is expressed in the germline and somatic gonad. Cross of JJ1473 and KK288. Unknown if unc-119(ed3) is still present or homozygous in background. Reference: Small LE & Dawes AT. Mol Biol Cell. 2017 Aug 1;28(16):2220-2231. Mutagen: Outcrossed: x0 Made by: Lawrence E. Small Received: 12/18/17 Ohio State University, Columbus, OH ----------------------------------------------------------------------------- Strain: ATD2 Species: Caenorhabditis elegans Genotype: par-2(or373) unc-119(ed3) III; zuIs45 V. Description: zuIs45 [nmy-2p::nmy-2::GFP + unc-119(+)] V. Temperature-sensitive maternal-effect lethal. Maintain at 15C. NMY-2::GFP is expressed in the germline and somatic gonad. Cross of JJ1473 and EU822. Unknown if unc-119(ed3) is still present or homozygous in background. NOTE: this strain was originally described as heterozygous for lin-2(e1309), but lin-2 has been lost; this strain is now homozygous wild-type for lin-2. Reference: Small LE & Dawes AT. Mol Biol Cell. 2017 Aug 1;28(16):2220-2231. Mutagen: Outcrossed: x0 Made by: Lawrence E. Small Received: 12/18/17 Ohio State University, Columbus, OH ----------------------------------------------------------------------------- Strain: ATD3 Species: Caenorhabditis elegans Genotype: lon-1(e185) par-3(e2074) unc-119(ed3) III; zuIs45 V; sDp3(III;f) Description: zuIs45 [nmy-2p::nmy-2::GFP + unc-119(+)] V. Pick wild-type to maintain. Worms carrying the sDp3 duplication are wild-type; animals that have lost the duplication are Lon Par (maternal effect lethal). Cross of JJ1473 and KK237. Unknown if unc-119(ed3) is still present or homozygous in background. Reference: Small LE & Dawes AT. Mol Biol Cell. 2017 Aug 1;28(16):2220-2231. Mutagen: Outcrossed: x0 Made by: Lawrence E. Small Received: 12/18/17 Ohio State University, Columbus, OH ----------------------------------------------------------------------------- Strain: ATD6 Species: Caenorhabditis elegans Genotype: par-6(zu222) unc-101(m1)/hIn1[unc-54(h1040)] I; unc-119(ed3) III; zuIs45 V. Description: zuIs45 [nmy-2p::nmy-2::GFP + unc-119(+)] V. Balanced worms are wild-type and segregate wild-type (heterozygotes), Coil Par (par-6 unc-101 homozygotes; maternal effect lethal), and paralyzed Unc (hIn1 homozygotes). Par phenotype is slightly leaky, but survivors are agametic. NMY-2::GFP is expressed in the germline and somatic gonad. Cross of JJ1473 and KK818. Unknown if unc-119(ed3) is still present or homozygous in background. Reference: Small LE & Dawes AT. Mol Biol Cell. 2017 Aug 1;28(16):2220-2231. Mutagen: Outcrossed: x0 Made by: Lawrence E. Small Received: 12/18/17 Ohio State University, Columbus, OH ----------------------------------------------------------------------------- Strain: ATD7 Species: Caenorhabditis elegans Genotype: par-2(ok1723)/sC1[dpy-1(s2170)], unc-119(ed3?) III; zuIs45 V. Description: zuIs45 [nmy-2p::nmy-2::GFP + unc-119(+)] V. Heterozygous worms are wild type and segregate wild type, Par (maternal effect lethal), and Dpy (sC1 homozygotes). Heterozygous and Par adults are indistinguishable on the plate. Maintain by picking wild-type worms and checking for correct segregation of progeny. NMY-2::GFP is expressed in the germline and somatic gonad. Cross of JJ1473 and VC1313. Unknown if unc-119(ed3) is still present or homozygous in background. Reference: Small LE & Dawes AT. Mol Biol Cell. 2017 Aug 1;28(16):2220-2231. Mutagen: Outcrossed: x0 Made by: Lawrence E. Small Received: 12/18/17 Ohio State University, Columbus, OH ----------------------------------------------------------------------------- Strain: ATU2301 Species: Caenorhabditis elegans Genotype: aceIs1; goeIs3. Description: goeIs3 [myo-3p::SL1::GCamP3.35::SL2::unc54 3'UTR + unc-119(+)]. aceIs1 [myo-3p::mitochondrial LAR-GECO + myo-2p::RFP]; likely inserted into LG II. Reporter expresses the calcium indicator cytosolic GCaMP3 and mitochondrial LAR-GECO in all body wall muscles. Mutagen: Outcrossed: x0 Made by: Atsushi Higashitani Received: 05/09/22 Graduate School of Life Sciences, Tohoku University ----------------------------------------------------------------------------- Strain: ATU3301 Species: Caenorhabditis elegans Genotype: ccIs4251 I; aceIs1. Description: ccIs4251 [myo-3p::GFP::LacZ::NLS + myo-3p::mitochondrial GFP + dpy-20(+)] I. aceIs1 [myo-3p::mitochondrial LAR-GECO + myo-2p::RFP]; likely inserted into LG II. Reporter expresses the calcium indicator mitochondrial LAR-GECO in all body wall muscles. Mutagen: Outcrossed: x0 Made by: Atsushi Higashitani Received: 05/09/22 Graduate School of Life Sciences, Tohoku University ----------------------------------------------------------------------------- Strain: ATU4301 Species: Caenorhabditis elegans Genotype: aceIs1. Description: aceIs1 [myo-3p::mitochondrial LAR-GECO + myo-2p::RFP]; likely inserted into LG II. Reporter expresses the calcium indicator mitochondrial LAR-GECO in all body wall muscles. Mutagen: Outcrossed: x0 Made by: Atsushi Higashitani Received: 05/09/22 Graduate School of Life Sciences, Tohoku University ----------------------------------------------------------------------------- Strain: AU1 Species: Caenorhabditis elegans Genotype: sek-1(ag1) X. Description: Enhanced susceptibility to pathogens (Esp). Egl-d. Nsy. Also called esp-2. Mutagen: EMS Outcrossed: x3 Made by: R. Feinbaum Received: 02/26/03 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: AU133 Species: Caenorhabditis elegans Genotype: agIs17 IV. Description: agIs17 [myo-2p::mCherry + irg-1p::GFP] IV. GFP in pharynx and intestine that turns on upon infection with pathogenic Pseudomonas aeruginosa strain PA14. Reference: Dunbar TL, et al. Cell Host Microbe. 2012 Apr 19;11(4):375-86. Mutagen: Outcrossed: x4 Made by: Emily Troemel Received: 09/09/13 UCSD, La Jolla CA, USA ----------------------------------------------------------------------------- Strain: AU147 Species: Caenorhabditis elegans Genotype: daf-16(mgDf47) I; glp-1(e2141) III. Description: Temperature sensitive sterility. Maintain at 15C. Mutagen: Outcrossed: x Made by: Sachiko Miyata Received: 04/16/08 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: AU166 Species: Caenorhabditis elegans Genotype: daf-16(mgDf47) I; fog-2(q71) V. Description: Temperature sensitive. Maintain by mating males & females at 15C. fog-2(q71) is male-female. Mutagen: Outcrossed: x Made by: Sachiko Miyata Received: 05/19/08 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: AU3 Species: Caenorhabditis elegans Genotype: nsy-1(ag3) II. Description: Enhanced susceptibility to pathogens (Esp). Nsy. Egl-d. Also called esp-8. Mutagen: EMS Outcrossed: x3 Made by: G. Alloing Received: 02/26/03 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: AU37 Species: Caenorhabditis elegans Genotype: glp-4(bn2) I; sek-1(km4) X. Description: Temperature sensitive sterility. Maintain at 15C. Enhanced sensitivity to pathogens. Mutagen: Outcrossed: x Made by: Terence Moy Received: 04/16/08 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: AU78 Species: Caenorhabditis elegans Genotype: agIs219 III. Description: agIs219 [T24B8.5p::GFP::unc-54 3' UTR + ttx-3p::GFP::unc-54 3' UTR] III. References: Shivers RP, et al. PLoS Genet. 2010 Apr 1;6(4):e1000892. Shivers RP, et al. Cell Host Microbe. 2009 Oct 22;6(4):321-30. Mutagen: Outcrossed: x3 Made by: Dennis Kim Received: 07/31/13 Boston Children's Hospital, Harvard Medical School ----------------------------------------------------------------------------- Strain: AU98 Species: Caenorhabditis elegans Genotype: inx-14(ag17) I. Description: Enhanced pathogen resistance. Mutagen: EMS Outcrossed: x5 Made by: Sachiko Miyata Received: 04/16/08 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: AUM1054 Species: C. elegans Genotype: gsk-3(tm2223) I/hT2[bli-4(e937) let-?(q782) qIs48] (I;III). Description: Homozygous sterile mutation balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP sterile tm2223 homozygotes. Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Furuta T, et al. Development. 2018 May 14;145(10). pii: dev161042. doi: 10.1242/dev.161042. Mutagen: Outcrossed: x5x Made by: Tokiko Furuta Received: 07/12/18 MD Anderson Cancer Center - Univ of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: AUM1535 Species: Caenorhabditis elegans Genotype: drsh-1(viz43)/tmC18[dpy-5(tmIs1200[myo-2p::mVenus])] I. Description: [D943G] substitution mutation in conserved residue within RNAse III domain. Balancer marked with myo-2p::Venus. Pick fertile wild-type (non-Dpy) Venus+ to maintain. drsh-1(viz43) homozygous animals display heterochronic phenotypes beginning at L3/L4 molt and typically burst at the vulva in L4. Heterozygotes are wild-type with pharyngeal Venus fluorescence, and segregate Venus+ heterozygotes, non-Venus viz-43 homozygotes, and Dpy Venus+ tmC18 homozygotes. Reference: Barish S, et al. Human Mol Genet. 2022 Aug 25;31(17):2934-2950. doi: 10.1093/hmg/ddac085. PMID: 35405010. Mutagen: Crispr/Cas9 Outcrossed: x5 Made by: J. H. Seemann Received: 06/16/23 MD Anderson Cancer Center - Univ of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: AUM1695 Species: Caenorhabditis elegans Genotype: prg-1(viz142[V5::mCherry::GSIGSLRSI::prg-1]) I. Description: V5 epitope and mCherry tags with a flexible linker inserted after the first 18 nt of the coding sequence of endogneous prg-1 locus. mCherry tagged PRG-1 primarily expressed and localized in both hermaphrodite and male gonad. Reference: Ortega J, et al. Sci. Adv.10,eadp0466(2024).DOI:10.1126/sciadv.adp0466 https://www.science.org/doi/10.1126/sciadv.adp0466 PMID: 39356768. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jake Seemann Received: 02/14/25 MD Anderson Cancer Center - Univ of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: AUM1747 Species: Caenorhabditis elegans Genotype: prg-1(viz142[V5::mCherry::GSIGSLRSI::prg-1] viz146[PAZ deletion]) I. Description: 282bp deletion (247aa-345aa) in PAZ domain of endogenously-tagged prg-1 locus. V5 epitope and mCherry tags with a flexible linker inserted after the first 18 nt of the coding sequence of endogneous prg-1 locus. mCherry tagged PRG-1 primarily expressed and localized in both hermaphrodite and male gonad. Reference: Ortega J, et al. Sci. Adv.10,eadp0466(2024).DOI:10.1126/sciadv.adp0466 https://www.science.org/doi/10.1126/sciadv.adp0466 PMID: 39356768. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jacob Ortega Received: 02/14/25 MD Anderson Cancer Center - Univ of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: AUM1760 Species: Caenorhabditis elegans Genotype: prg-1(viz142[V5::mCherry::GSIGSLRSI::prg-1] viz151[D583A]) I. Description: Inactive RNase mutation of endogenously-tagged prg-1 locus. V5 epitope and mCherry tags with a flexible linker inserted after the first 18 nt of the coding sequence of endogneous prg-1 locus. mCherry tagged PRG-1 primarily expressed and localized in both hermaphrodite and male gonad. Reference: Ortega J, et al. Sci. Adv.10,eadp0466(2024).DOI:10.1126/sciadv.adp0466 https://www.science.org/doi/10.1126/sciadv.adp0466 PMID: 39356768. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jake Seemann Received: 02/14/25 MD Anderson Cancer Center - Univ of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: AUM1787 Species: Caenorhabditis elegans Genotype: cosa-1(viz154[GFP::cosa-1]) III. Description: GFP tag inserted at N-terminus of endogenous cosa-1 locus. Expressed primarily in both hermaphrodite and male gonad. Reference: Ortega J, et al. Sci. Adv.10,eadp0466(2024).DOI:10.1126/sciadv.adp0466 https://www.science.org/doi/10.1126/sciadv.adp0466 PMID: 39356768. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Jacob Ortega Received: 02/14/25 MD Anderson Cancer Center - Univ of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: AUM1811 Species: Caenorhabditis elegans Genotype: plk-3(viz156[plk-3::GGSGGGSGGGSG::GFP]) IV. Description: GFP tag with linker sequence inserted at C-terminus of endogenous plk-3 locus. Expressed primarily in both male and hermaphrodite gonad. Reference: Ortega J, et al. Sci. Adv.10,eadp0466(2024).DOI:10.1126/sciadv.adp0466 https://www.science.org/doi/10.1126/sciadv.adp0466 PMID: 39356768. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jake Seemann Received: 02/14/25 MD Anderson Cancer Center - Univ of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: AUM1830 Species: Caenorhabditis elegans Genotype: sart-3(tm6688)/tmC9 [F36H1.2(tmIs1221)] IV. Description: Homozygous sterile deletion balanced by tmC9 [F36H1.2(tmIs1221[myo-2p::Venus])]. Heterozygotes are wild-type Venus+ in pharynx, and segregate wild-type Venus+ heterozygotes, non-Venus Sterile (tm6688 homozygotes), and Venus+ Mec Unc (tmC9 homozygotes). Pick viable fertile Venus+ animals and check for correct segregation of progeny to maintain. Reference: Furuta T & Arur S. 2023. sart-3 functions to regulate germline sex determination in C. elegans. microPublication Biology. PubMed ID: 37206989. Mutagen: Outcrossed: x5 Made by: Tokiko Furuta Received: 06/16/23 MD Anderson Cancer Center - Univ of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: AUM1863 Species: Caenorhabditis elegans Genotype: sart-3(tm15993)/tmC9 [F36H1.2(tmIs1221)] IV. Description: Homozygous lethal deletion balanced by tmC9 [F36H1.2(tmIs1221[myo-2p::Venus])]. Heterozygotes are wild-type Venus+ in pharynx, and segregate wild-type Venus+ heterozygotes, non-Venus Sterile (tm15993 homozygotes), and Venus+ Mec Unc (tmC9 homozygotes). Pick viable fertile Venus+ animals and check for correct segregation of progeny to maintain. 93% of tm15993 homozygotes die before adulthood and those that escape are sterile. Reference: Furuta T & Arur S. 2023. sart-3 functions to regulate germline sex determination in C. elegans. microPublication Biology. PubMed ID: 37206989. Mutagen: Outcrossed: x5 Made by: Tokiko Furuta Received: 06/16/23 MD Anderson Cancer Center - Univ of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: AUM1870 Species: C. elegans Genotype: ZK813.1(viz166[fln-1p::ZK813.1]) X. Description: The endogenous promoter of ZK813.1 (1046 bp) was replaced with the fln-1 promoter (947 bp) to limit the expression of ZK813.1 to the spermatheca and allow analysis of RNA transfer from soma to oocytes. Reference: Trimmer KA, et al. Cell Rep. 2023 May 23;42(6):112544. doi: 10.1016/j.celrep.2023.112544. PMID: 37227820. . Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jake Seemann Received: 06/16/23 MD Anderson Cancer Center - Univ of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: AUM1880 Species: Caenorhabditis elegans Genotype: prg-1(viz142[V5::mCherry::GSIGSLRSI::prg-1]) I; plk-3(viz172[plk-3 delta 21u-10935] viz156[plk-3::GGSGGGSGGGSG::GFP]) IV. Description: viz172 is a series of point mutations at that piRNA binding site in endogenously-tagged plk-3 locus. GFP tag with linker sequence inserted at C-terminus of endogenous plk-3 locus. V5 epitope and mCherry tags with a flexible linker inserted after the first 18 nt of the coding sequence of endogneous prg-1 locus. Both tagged transgenes are primarily expressed and localized in both hermaphrodite and male gonad. Eight silent mutations in 21u-10935 binding site. Original plk-3: CTCAGTCGTATCGAATATGCCCAA viz172: CTgtccCGTATCGAgTAcGCaCAg Reference: Ortega J, et al. Sci. Adv.10,eadp0466(2024).DOI:10.1126/sciadv.adp0466 https://www.science.org/doi/10.1126/sciadv.adp0466 PMID: 39356768. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jake Seemann Received: 02/14/25 MD Anderson Cancer Center - Univ of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: AUM2023 Species: Caenorhabditis elegans Genotype: daf-2(e1370) unc-119(ed3) III; vizIs23. Description: vizIs23 [pie-1p::GFP::daf-2(WT)::pie-1 3'UTR + unc-119(+)]. Maintain at 15C; pick superficially wild-type animals to avoid silencing of the transgene. pie-1 driven DAF-2 coding region with GFP transgene rescues the germline defects of daf-2(e1370). Slow growing. The transgene is sometimes silenced in the germline resulting in dauerunc animals at 25C. Reference: Lopez AL 3rd, et al. Dev Cell. 2013 Oct 28;27(2):227-40. Mutagen: Outcrossed: x2 Made by: Jessica (Jie) Chen Received: 08/15/14 MD Anderson Cancer Center - Univ of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: AUM2059 Species: C. elegans Genotype: vizSi20 II; unc-119(ed3) III. Description: vizSi20 [mex-5p::GFP::gsk-3 (K65A,E77A,D161A,D180A)::tbb-2 3’UTR + unc-119(+)] II. Superficially wild-type. vizSi20 was inserted into Chr II ttTi5605 using MosSci. GSK-3 cDNA was rendered kinase dead by replacing K65, E77, D161 and D180 to alanine. The transgene does not rescue gsk-3 sterility or embryonic lethality defects. Reference: Furuta T, et al. Development. 2018 May 14;145(10). pii: dev161042. doi: 10.1242/dev.161042. Mutagen: Outcrossed: x3x Made by: Tokyo Furuta Received: 07/12/18 MD Anderson Cancer Center - Univ of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: AUM2071 Species: C. elegans Genotype: vizSi32 II; unc-119(ed3) III. Description: vizSi32 [cdk-2p(intron)::GFP::tbb-2 3’ UTR + unc-119(+)] II. vizSi32 was inserted into ttTi5605 on Chr II using MosSci. Intron 1 of cdk-2 drives drives GFP expression in this transgene. Reference: Furuta T, et al. Development. 2018 May 14;145(10). pii: dev161042. doi: 10.1242/dev.161042. Mutagen: Outcrossed: x2x Made by: Tokyo Furuta Received: 07/12/18 MD Anderson Cancer Center - Univ of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: AUM2073 Species: C. elegans Genotype: vizSi34 II; unc-119(ed3) III. Description: vizSi34 [cdk-2p::GFP::tbb-2 3’ UTR + unc-119(+)] II. Superficially wild-type. vizSi34 was inserted into ttTi5605 on Chr II using MosSci. Predicted cdk-2 promoter (from WormBase) drives GFP expression. Reference: Furuta T, et al. Development. 2018 May 14;145(10). pii: dev161042. doi: 10.1242/dev.161042. Mutagen: Outcrossed: x4x Made by: Tokyo Furuta Received: 07/12/18 MD Anderson Cancer Center - Univ of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: AV106 Species: Caenorhabditis elegans Genotype: spo-11(ok79) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Uncs (heterozygotes), non-Unc spo-11 homozygotes, and dead eggs (nT1 homozygotes). spo-11 homozygotes produce an average of ~200 fertilized eggs but only about 0.1 progeny survive to adulthood. When mated to N2 males, spo-11 homozygotes will produce at least 5-10 cross progeny. Mutagen: Diepoxybutane Outcrossed: x8 Made by: Abby Dernburg Received: 10/30/98 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AV112 Species: Caenorhabditis elegans Genotype: mre-11(ok179) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Uncs (heterozygotes), non-Unc mre-11 homozygotes, and dead eggs (nT1 homozygotes). mre-11 homozygotes produce about 200 fertilized eggs but only about 2-3% of these eggs survive to adulthood (this mutation cannot be maintained in a homozygous condition). Occasionally non-Unc progeny that do not demonstrate the mre-11(ok179) mutant phenotype arise when grown in large liquid cultures. mre-11 is the predicted gene ZC302.1 Mutagen: Outcrossed: x Made by: Deletion Consortium Received: 12/04/00 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AV115 Species: Caenorhabditis elegans Genotype: msh-5(me23) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Uncs (heterozygotes), non-Unc msh-5 homozygotes, and dead eggs (nT1 homozygotes). msh-5 homozygotes give 97.9% dead eggs; of those that hatch, 42% are male. Mutagen: EMS Outcrossed: x Made by: Received: 03/06/02 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AV125 Species: Caenorhabditis elegans Genotype: spe-8(hc40) I; dpy-4(e1166) IV. Description: Can be maintained by chunking or setting up male/hermaphrodite crosses. Dpy. Mutagen: Outcrossed: x Made by: Gillian Stanfield Received: 10/30/06 University of Utah, Salt Lake City UT, USA ----------------------------------------------------------------------------- Strain: AV146 Species: Caenorhabditis elegans Genotype: chk-2(me64) rol-9(sc148)/unc-51(e369) rol-9(sc148) V. Description: Heterozygotes are fertile Rollers and segregate fertile non-Rollers (heterozygote), Unc Rollers (unc-51 homozygotes), and non-Unc Rollers that give 96-97% dead eggs (a high % of the survivors are males). Mutagen: Outcrossed: x Made by: Amy MacQueen Received: 07/11/01 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AV157 Species: Caenorhabditis elegans Genotype: spo-11(me44)/nT1 [unc-?(n754) let-? qIs50] (IV;V). Description: Balanced heterozygotes are GFP+ Unc and segregate GFP+ Unc (heterozygotes), non-GFP non-Unc spo-11(me44) homozygotes, and dead eggs (nT1 homozygotes). spo-11(me44) homozygotes are viable and produce more than 90% dead eggs (a large fraction of the survivors are males — strong Him phenotype); cytologically they lack chiasmata in diakinesis-stage oocytes and lack RAD-51 foci. Maintain by picking Unc. Mutagen: EMS Outcrossed: x>2 Made by: Gregory Chin Received: 08/27/07 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AV221 Species: Caenorhabditis elegans Genotype: unc-119(ed3) meT8 (III); meIs4 meT8 (IV); meIs1. Description: meIs1 [pie-1p::GFP::lacI + unc-119(+)]. meIs4 [lac-O + rol-6(su1006) + lacO] IV. Pick Rol worms to maintain. This strain throws both Rol and non-Rol worms, seemingly due to random silencing of rol-6(su1006) in the lacO array, meIs4. The strain expresses GFP::LacI in the gonad and embryos that is observed as foci (of lacO target) and nuclear haze. The expression level of GFP::LacI occasionally becomes low possibly due to random silencing of meIs1. If this happens, heat shock the strain at 25°C for 3 days, and pick a clone that exhibits bright GFP signals. Even at the highest expression level, GFP signal is too weak to detect with a fluorescent dissection microscope, and it is necessary to use a regular compound fluorescent microscope with an oil immersion 60X or 100X objective. The NA of the objective should be higher than 1.4. Reference: Bilgir C, et al. G3 (Bethesda). 2013 Mar 11. pii: g3.112.005165v1. Mutagen: Outcrossed: x0 Made by: Kentaro Nabeshima Received: 04/30/14 University of Michigan, Ann Arbor, MI ----------------------------------------------------------------------------- Strain: AV267 Species: Caenorhabditis elegans Genotype: syp-3(me42)/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT and GFP+. Segregate syp-3(me42) homozygotes that are non-GFP and lay mostly dead eggs; these mutants form abnormal synaptonemal complex formation during meiosis. Homozygous hT2[bli-4 let-? qIs48] animals are inviable. Mutagen: EMS Outcrossed: x4 Made by: J Engebrecht Received: 09/28/07 Harvard Medical School, Boston, MA ----------------------------------------------------------------------------- Strain: AV271 Species: Caenorhabditis elegans Genotype: him-3(me80)/nT1 [unc-?(n754) let-? qIs50] (IV;V). Description: Balanced heterozygotes are GFP+ Unc and segregate GFP+ Unc (heterozygotes), non-GFP non-Unc him-3(me80) homozygotes, and dead eggs (nT1 homozygotes). him-3(me80) homozygotes are viable and non-Unc. They produce more than 85% dead eggs and a large fraction (11%) of the survivors are males (Him phenotype). Cytologically they exhibit a reduced level of HIM-3 loading and fewer stretches of SYP-1 than WT. In diakinesis-stage oocytes, they contain a mixture of bivalents and univalents. Maintain by picking Unc. Mutagen: EMS Outcrossed: x>2 Made by: Kentaro Nabeshima Received: 08/27/07 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AV276 Species: Caenorhabditis elegans Genotype: syp-2(ok307) V/nT1 [unc-?(n754) let-?(m435)] (IV;V). Description: Balanced heterozygotes are Unc and segregate Unc (heterozygotes), non-Unc syp-2(ok307) homozygotes, and dead eggs (nT1 homozygotes). syp-2(ok307) homozygotes are viable and non-Unc. They produce 96% dead eggs and 44% males; cytologically they lack chiasmata in diakinesis-stage oocytes, exhibit persistent polarized nuclear organization during earlier meiotic prophase, lack synaptonemal complexes, and exhibit unstable pairing of homologous chromosomes. Mutagen: UV/TMP Outcrossed: x6 Made by: Monica Colaiacovo Received: 09/16/04 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AV280 Species: Caenorhabditis elegans Genotype: unc-119(e2498) III; him-17(ok424) V; meIs5. Description: meIs5 [him-17::GFP + unc-119(+)]. him-17::GFP is expressed in the germline. meIs5 not mapped. Mutagen: Microparticle Bombardment Outcrossed: x Made by: Kirthi Reddy Received: 09/16/04 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AV285 Species: Caenorhabditis elegans Genotype: swm-1(me66) him-5(e1490) V. Description: Sperm activation in virgin males. Poor sperm transfer. Mutagen: EMS Outcrossed: x6 Made by: Gillian Stanfield Received: 05/25/06 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AV307 Species: Caenorhabditis elegans Genotype: syp-1(me17) V/nT1 [unc-?(n754) let-? qIs50] (IV;V). Description: Balanced heterozygotes are GFP+ Unc and segregate GFP+ Unc (heterozygotes), non-GFP non-Unc syp-1(me17) homozygotes, and dead eggs (nT1 homozygotes). syp-1(me17) homozygotes produce 95% dead embryos and 38% males. Cytologically they lack chiasmata in diakinesis-stage oocytes, exhibit persistent polarized nuclear organization during earlier meiotic prophase, lack synaptonemal complexes, and exhibit unstable pairing of homologous chromosomes. qIs50 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter (F22B7.9) driving GFP in the intestine. Mutagen: EMS Outcrossed: x>2 Made by: Amy MacQueen Received: 09/25/03 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AV308 Species: Caenorhabditis elegans Genotype: him-14(it21)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are wild-type and segregate wild-type heterozygotes, DpyUncs (mnC1 homozygotes), and him-14 homozygotes that produce >95% dead embryos and 45% males. Among these surviving progeny, cytologically they have 12 univalents in diakinesis-stage oocytes owing to a failure to form crossovers during meiosis. Mutagen: EMS Outcrossed: x Made by: Received: 11/04/05 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AV311 Species: Caenorhabditis elegans Genotype: dpy-18(e364) unc-3(e151) meT7 (III;X;IV). Description: Dpy. Unc. meT7 is an end-to-end-to-end fusion of chromosomes III, X, and V. The right end of III is fused to the left end of X, and the right end of X is fused to the left end of IV. Constructed by crossing eT5 and mnT12. meT7 homozygotes produce 92% viable progeny. meT7 heterozygotes are Him and produce many dead eggs. Mutagen: Outcrossed: x>1 Made by: Kenneth Hillers Received: 12/05/03 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AV322 Species: Caenorhabditis elegans Genotype: swm-1(me87) him-5(e1490) V. Description: Sperm activation in virgin males. Very poor sperm transfer. Mutagen: EMS Outcrossed: x6 Made by: Gillian Stanfield Received: 05/25/06 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AV38 Species: Caenorhabditis elegans Genotype: mnDp66 (X;I); meDf2 X. Description: Produces 31% XO male self progeny; nondisjunction is correlated with a high frequency of achiasmate X chromosomes in oocyte nuclei, and a reduced frequency of X chromosome crossovers. meDf2 disrupts the function of the cis-acting X chromosome meiotic pairing center. meDf2/+ heterozygotes produce 4-6% XO progeny, so the presence of meDf2 can be followed in heterozygotes by this weak Him phenotype. Mutagen: Gamma Rays Outcrossed: x2 Made by: Anne Villeneuve Received: 06/16/97 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AV39 Species: Caenorhabditis elegans Genotype: mnDp66 (X;I); meDf3 X. Description: Produces 32% XO male self progeny; nondisjunction is correlated with a high frequency of achiasmate X chromosomes in oocyte nuclei, and a reduced frequency of X chromosome crossovers. meDf3 disrupts the function of the cis-acting X chromosome meiotic pairing center. meDf3/+ heterozygotes produce 4-6% XO progeny, so the presence of meDf3 can be followed in heterozygotes by this weak Him phenotype. Mutagen: Gamma Rays Outcrossed: x2 Made by: Anne Villeneuve Received: 06/16/97 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AV40 Species: Caenorhabditis elegans Genotype: mnDp66 (X;I); meDf4 X. Description: Produces 27% XO male self progeny; nondisjunction is correlated with a high frequency of achiasmate X chromosomes in oocyte nuclei, and a reduced frequency of X chromosome crossovers. meDf4 disrupts the function of the cis-acting X chromosome meiotic pairing center. meDf4/+ heterozygotes produce 4-6% XO progeny, so the presence of meDf4 can be followed in heterozygotes by this weak Him phenotype. Mutagen: Gamma Rays Outcrossed: x2 Made by: Anne Villeneuve Received: 06/16/97 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AV41 Species: Caenorhabditis elegans Genotype: mnDp66 (X;I); meDf5 X. Description: Produces 32% XO male self-progeny; nondisjunction is correlated with a high frequency of achiasmate X chromosomes in oocyte nuclei, and a reduced frequency of X chromosome crossovers. meDf5 disrupts the function of the cis-acting X chromosome meiotic pairing center. meDf5/+ heterozygotes produce 4-6% XO progeny, so the presence of meDf5 can be followed in heterozygotes by this weak Him phenotype. Mutagen: Gamma Rays Outcrossed: x2 Made by: Anne Villeneuve Received: 06/16/97 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AV473 Species: Caenorhabditis elegans Genotype: rad-50(ok197) V/nT1 [qIs51] (IV;V). Description: qIs51 [myo-2p::GFP + pes-10p::GFP + F22B7.9p::GFP]. Heterozygotes are wild-type GFP+ and segregate non-GFP ok197 homozygotes (viable, sterile), wild-type GFP+ heterozygotes, and arrested nT1[qIs51] aneuploids. rad-50 homozygotes are viable, produce more than 95% dead eggs and a large fraction of the survivors are male (Him phenotype). Pick wild-type GFP+ and check for correct segregation of progeny to maintain. Reference: Hayashi M, et al. PLoS Genet. 2007 Nov;3(11):e191. Mutagen: Outcrossed: x4 Made by: Michiko Hayashi Received: 04/07/15 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AV477 Species: C. elegans Genotype: dsb-2(me96) II. Description: Age-dependent defect in meiotic double-strand break formation. Homozygous mutants produce elevated frequency of males and dead embryos resulting from defects in meiotic chromosome segregation. The frequency of both males and dead embryos increases in later broods. Reference: Rosu S, et al. PLoS Genet. 2013;9(8):e1003674. Mutagen: EMS Outcrossed: x4 Made by: Simona Rosu Received: 08/27/18 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AV51 Species: Caenorhabditis elegans Genotype: me8 X. Description: Homozygotes produce 10-15% XO male self progeny; nondisjuction is correlated with an increased frequency of achiasmate X chromosomes in oocyte nuclei, and an unaltered distribution of X chromosome crossovers. Heterozygotes produce 1-2% male self-progeny. Homozygotes (and XO hemizygotes) are slower growing than WT; reduced male mating efficiency. me8 disrupts the function of the cis-acting X chromosome meiotic pairing center. Molecular studies show that the me8 chromosome carries a terminal deletion that removes >70 kb from the left end of the X chromosome, including the endogenous telomere; further, a segment of chromosome V has been translocated to the left end of X, and a new telomere has been added de novo to the end of the translocated segment. Mutagen: EMS Outcrossed: x4 Made by: Anne Villeneuve Received: 06/16/97 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AV554 Species: Caenorhabditis elegans Genotype: dpy-13(e184) unc-5(ox171) IV/nT1 [qIs51] (IV;V); krIs14 V/nT1 [qIs51] (IV;V). Description: krIs14 [hsp-16.48p::MosTransposase + lin-15B + unc-122p::GFP]. Heterozygotes are WT with GFP (GFP+ coelomocytes & GFP+ pharynx), and segregate WT GFP, arrested nT1[qIs51] aneuploids, and Dpy Unc homozygotes (GFP+ pharynx & GFP+ coelomocytes). Homozygous nT1[qIs51] inviable. Pick WT with GFP (GFP+ pharynx & GFP+ coelomocytes) and check for correct segregation of progeny to maintain. References: Robert V & Bessereau JL. EMBO J. 2007 Jan 10;26(1):170-83. doi: 10.1038/sj.emboj.7601463. PMID: 17159906. Rosu S, et al. Science. 2011 Dec 2;334(6060):1286-9. doi: 10.1126/science.1212424. PMID: 22144627. Toraason E, et al. Elife. 2024 Aug 8;13:e80687. doi: 10.7554/eLife.80687. PMID: 39115289. Mutagen: Outcrossed: x0 Made by: Simona Rosu Received: 02/06/25 University of Oregon, Eugene, OR ----------------------------------------------------------------------------- Strain: AV630 Species: Caenorhabditis elegans Genotype: meIs8 II. Description: meIs8 [pie-1p::GFP::cosa-1 + unc-119(+)] II. Transgene contains a combination of cDNA and genomic sequences of cosa-1 including 212 bp of 3'UTR. GFP is expressed in the adult germline as 6 bright foci per nucleus (one per chromosome pair) from late pachytene through diplotene stages. Reference: Yokoo R, et al. Cell. 2012 Mar 30;149(1):75-87. Mutagen: Bombardment Outcrossed: x1 Made by: Rayka Yokoo Received: 04/12/12 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AV828 Species: C. elegans Genotype: nbs-1(me102) meIs8/mIn1 [mIs14 dpy-10(e128)] II. Description: meIs8 [pie-1p::GFP::cosa-1 + unc-119(+)] II. Transgene contains a combination of cDNA and genomic sequences of cosa-1 including 212 bp of 3'UTR. GFP is expressed in the adult germline as 6 bright foci per nucleus (one per chromosome pair) from late pachytene through diplotene stages. Homozygous sterile mutation balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP me103 homozygotes (sterile adult). Pick WT dim GFP and check for correct segregation of progeny to maintain. nbs-1(me103) homozygotes have frayed and aggregated chromosomes at diakinesis of meiosis I. References: Girard C, et al. Proc Natl Acad Sci U S A. 2018 May 8;115(19):E4443-E4452. Yokoo R, et al. Cell. 2012 Mar 30;149(1):75-87. Mutagen: EMS Outcrossed: x4 Made by: Chloe Girard, Baptiste Roelens, Karl A. Zawadzki Received: 12/28/18 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AV860 Species: C. elegans Genotype: nbs-1(me103)/mIn1 [mIs14 dpy-10(e128)] II. Description: Homozygous sterile mutation balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP me103 homozygotes (sterile adult). Pick WT dim GFP and check for correct segregation of progeny to maintain. nbs-1(me103) homozygotes have frayed and aggregated chromosomes at diakinesis of meiosis I. Reference: Girard C, et al. Proc Natl Acad Sci U S A. 2018 May 8;115(19):E4443-E4452. Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Chloe Girard Received: 12/28/18 Stanford University Medical School, Stanford, CA ----------------------------------------------------------------------------- Strain: AVS310 Species: Caenorhabditis elegans Genotype: artEx27. Description: artEx27 [hpk-1p::hpk-1::GFP + rol-6(su1006)]. Full-length C-terminal hpk-1::GFP fusion. Broad pattern of developmental GFP expression in the intestine, hypodermal seam cells, and neurons. Reference: Das R, et al. PLoS Genet. 2017 Oct 16;13(10):e1007038. doi: 10.1371/journal.pgen.1007038. PMID: 29036198; PMCID: PMC5658188. Mutagen: Outcrossed: x0 Made by: Ritikas Das Received: 09/10/20 University of Rochester, Rochester, NY ----------------------------------------------------------------------------- Strain: AVS394 Species: C. elegans Genotype: artEx12. Description: artEx12 [hpk-1p::GFP + rol-6(su1006)]. Pick Rollers to maintain. Transcriptional fusion of hpk-1 promoter with GFP. Reference: Das R, et al. PLoS Genet. 2017 Oct 16;13(10):e1007038. doi: 10.1371/journal.pgen.1007038. PMID: 29036198; PMCID: PMC5658188. Mutagen: Outcrossed: x0 Made by: Ritikas Das Received: 09/10/20 University of Rochester, Rochester, NY ----------------------------------------------------------------------------- Strain: AVS397 Species: C elegans Genotype: gpIs1; artEx35. Description: gpIs1 [hsp-16.2p::GFP]. artEx35 [sur-5p::hpk-1::CFP + myo-2p::mCherry)]. Pick animals with red pharynx to maintain. Inducible GFP fluorescence after >1 hour heat shock. Reference: Das R, PLoS Genet. 2017 Oct 16;13(10):e1007038. doi: 10.1371/journal.pgen.1007038. PMID: 29036198; PMCID: PMC5658188. Mutagen: Outcrossed: x0 Made by: Ritikas Das Received: 09/10/20 University of Rochester, Rochester, NY ----------------------------------------------------------------------------- Strain: AVS408 Species: Caenorhabditis elegans Genotype: artEx31. Description: artEx31 [sur-5p::hpk-1::CFP + myo-2p::mCherry)]. Pick mCherry+ animals to maintain. sur-5 driven over-expression of hpk-1. Reference: Das R, et al. PLoS Genet. 2017 Oct 16;13(10):e1007038. doi: 10.1371/journal.pgen.1007038. PMID: 29036198; PMCID: PMC5658188. Mutagen: Outcrossed: x0 Made by: Ritikas Das Received: 09/10/20 University of Rochester, Rochester, NY ----------------------------------------------------------------------------- Strain: AVS411 Species: C elegans Genotype: artEx30. Description: artEx30 [hpk-1p::hpk-1::tdTomato + hsf-1p::hsf-1::GFP + rol-6 (su1006)]. Pick Rollers to maintain. Reference: Das R, PLoS Genet. 2017 Oct 16;13(10):e1007038. doi: 10.1371/journal.pgen.1007038. PMID: 29036198; PMCID: PMC5658188. Mutagen: Outcrossed: x0 Made by: Ritikas Das Received: 09/10/20 University of Rochester, Rochester, NY ----------------------------------------------------------------------------- Strain: AVS413 Species: Caenorhabditis elegans Genotype: hpk-1(pk1393) X; artEx29. Description: artEx29 [hpk-1p::hpk-1::GFP + rol-6(su1006)]. Full-length C-terminal hpk-1::GFP fusion transgene rescues the progeric phenotype of hpk-1(pk1393). Reference: Das R, et al. PLoS Genet. 2017 Oct 16;13(10):e1007038. doi: 10.1371/journal.pgen.1007038. PMID: 29036198; PMCID: PMC5658188. Mutagen: Outcrossed: x9 Made by: Ritikas Das Received: 09/10/20 University of Rochester, Rochester, NY ----------------------------------------------------------------------------- Strain: AWR41 Species: C. elegans Genotype: lin-35(kea7[lin-35p::degron::GFP::lin-35]) I. Description: N-terminal degron::GFP tag was inserted into the endogenous lin-35 locus. Generated in N2 background. Reference: Willis AR, et al. Sci Adv. 2021 May 5;7(19):eabf3114. PMID: 33952520 [NOTE: lin-35(kea7) is an N-terminal tag; the methods section of the paper incorrectly describes the tag as a C-terminal insertion.] Mutagen: Outcrossed: x3 Made by: Winnie Zhao Received: 05/19/21 University of Toronto ----------------------------------------------------------------------------- Strain: AWR45 Species: C. elegans Genotype: pals-22(kea8[pals-22::GFP::degron]) III. Description: C-terminal GFP::degron tag was inserted into the endogenous pals-22 locus. Generated in N2 background. Reference: Willis AR, et al. Sci Adv. 2021 May 5;7(19):eabf3114. PMID: 33952520 [NOTE: pals22(kea8) is a C-terminal tag; the methods section of the paper incorrectly describes the tag as an N-terminal insertion.] Mutagen: Outcrossed: x3 Made by: Winnie Zhao Received: 05/19/21 University of Toronto ----------------------------------------------------------------------------- Strain: AWR54 Species: C. elegans Genotype: lin-35(kea7[lin-35p::degron::GFP::lin-35]) I; ieSi57 II. Description: ieSi57 [eft-3p::TIR1::mRuby::unc-54 3’UTR + Cbr-unc-119(+)] II. N-terminal degron::GFP tag was inserted into the endogenous lin-35 locus. A single copy insertion of eft-3p::TIR1::mRuby allows auxin-inducible degradation of LIN-35 in somatic tissues. Generated in N2 background. Reference: Willis AR, et al. Sci Adv. 2021 May 5;7(19):eabf3114. PMID: 33952520 [NOTE: lin-35(kea7) is an N-terminal tag; the methods section of the paper incorrectly describes the tag as a C-terminal insertion.] Mutagen: Outcrossed: x3 Made by: Winnie Zhao Received: 05/19/21 University of Toronto ----------------------------------------------------------------------------- Strain: AWR56 Species: C. elegans Genotype: lin-35(kea7[lin-35p::degron::GFP::lin-35]) I; ieSi64 II. Description: ieSi64 [gld-1p::TIR1::mRuby::unc-54 3’UTR + Cbr-unc-119(+)] II. N-terminal degron::GFP tag was inserted into the endogenous lin-35 locus. A single copy insertion of gld-1p::TIR1::mRuby allows for auxin inducible degradation of LIN-35 in the germline. Generated in N2 background. Reference: Willis AR, et al. Sci Adv. 2021 May 5;7(19):eabf3114. PMID: 33952520 [NOTE: lin-35(kea7) is an N-terminal tag; the methods section of the paper incorrectly describes the tag as a C-terminal insertion.] Mutagen: Outcrossed: x3 Made by: Winnie Zhao Received: 05/19/21 University of Toronto ----------------------------------------------------------------------------- Strain: AWR58 Species: C. elegans Genotype: lin-35(kea7[lin-35p::degron::GFP::lin-35]) I; keaSi10 II. Description: keaSi10 [rpl-28p::TIR1::mRuby::unc-54 3’UTR + Cbr-unc-119(+)] II. N-terminal degron::GFP tag was inserted into the endogenous lin-35 locus. A single copy insertion of rpl-28::TIR1::mRuby allows auxin-inducible degradation of LIN-35 throughout the animal. Generated in N2 background. Reference: Willis AR, et al. Sci Adv. 2021 May 5;7(19):eabf3114. PMID: 33952520 [NOTE: lin-35(kea7) is an N-terminal tag; the methods section of the paper incorrectly describes the tag as a C-terminal insertion.] Mutagen: Outcrossed: x3 Made by: Winnie Zhao Received: 05/19/21 University of Toronto ----------------------------------------------------------------------------- Strain: AWR59 Species: C. elegans Genotype: keaSi10 II; pals22(kea8[pals-22::GFP::degron]) III. Description: keaSi10 [rpl-28p::TIR1::mRuby::unc-54 3’UTR + Cbr-unc-119(+)] II. C-terminal GFP::degron tag was inserted into the endogenous pals-22 locus. A single copy insertion of rpl-28::TIR1::mRuby allows auxin-inducible degradation of PALS-22 throughout the animal. Generated in N2 background. Reference: Willis AR, et al. Sci Adv. 2021 May 5;7(19):eabf3114. PMID: 33952520 [NOTE: pals22(kea8) is a C-terminal tag; the methods section of the paper incorrectly describes the tag as an N-terminal insertion.] Mutagen: Outcrossed: x3 Made by: Winnie Zhao Received: 05/19/21 University of Toronto ----------------------------------------------------------------------------- Strain: AWR61 Species: C. elegans Genotype: keaSi11 II; pals22(kea8[pals-22::GFP::degron]) III. Description: keaSi11 [vit-2p::TIR1::mRuby::unc-54 3’UTR + Cbr-unc-119(+)] II. C-terminal GFP::degron tag was inserted into the endogenous pals-22 locus. A single copy insertion of vit-2p::TIR1::mRuby allows for auxin inducible degradation in the adult intestine. Generated in N2 background. Reference: Willis AR, et al. Sci Adv. 2021 May 5;7(19):eabf3114. PMID: 33952520 [NOTE: pals22(kea8) is a C-terminal tag; the methods section of the paper incorrectly describes the tag as an N-terminal insertion.] Mutagen: Outcrossed: x3 Made by: Winnie Zhao Received: 05/19/21 University of Toronto ----------------------------------------------------------------------------- Strain: AWR62 Species: C. elegans Genotype: keaSi9 II; pals22(kea8[pals-22::GFP::degron]) III. Description: keaSi9 [myo-3p::TIR1::mRuby::unc-54 3’UTR + Cbr-unc-119(+)] II. C-terminal GFP::degron tag was inserted into the endogenous pals-22 locus. A single copy insertion of myo-3p::TIR1::mRuby allows for auxin inducible degradation in the body wall muscles. Generated in N2 background. Reference: Willis AR, et al. Sci Adv. 2021 May 5;7(19):eabf3114. PMID: 33952520 [NOTE: pals22(kea8) is a C-terminal tag; the methods section of the paper incorrectly describes the tag as an N-terminal insertion.] Mutagen: Outcrossed: x3 Made by: Winnie Zhao Received: 05/19/21 University of Toronto ----------------------------------------------------------------------------- Strain: AWR63 Species: C. elegans Genotype: keaSi12 II; pals22(kea8[pals-22::GFP::degron]) III. Description: keaSi12 [dpy-7p::TIR1::mRuby::unc-54 3’UTR + Cbr-unc-119(+)] II. C-terminal GFP::degron tag was inserted into the endogenous pals-22 locus. A single copy insertion of dpy-7p::TIR1::mRuby allows for auxin inducible degradation in the epidermis. Generated in N2 background. Reference: Willis AR, et al. Sci Adv. 2021 May 5;7(19):eabf3114. PMID: 33952520 [NOTE: pals22(kea8) is a C-terminal tag; the methods section of the paper incorrectly describes the tag as an N-terminal insertion.] Mutagen: Outcrossed: x3 Made by: Winnie Zhao Received: 05/19/21 University of Toronto ----------------------------------------------------------------------------- Strain: AWR64 Species: C. elegans Genotype: kea15 II; pals22(kea8[pals-22::GFP::degron]) III. Description: kea15 [rgef-1p::TIR1::mRuby::unc-54 3’UTR + Cbr-unc-119(+)] II. C-terminal GFP::degron tag was inserted into the endogenous pals-22 locus. A single copy insertion of rgef-1p::TIR1::mRuby allows for auxin inducible degradation in neurons. Generated in N2 background. Reference: Willis AR, et al. Sci Adv. 2021 May 5;7(19):eabf3114. PMID: 33952520 [NOTE: pals22(kea8) is a C-terminal tag; the methods section of the paper incorrectly describes the tag as an N-terminal insertion.] Mutagen: Outcrossed: x3 Made by: Winnie Zhao Received: 05/19/21 University of Toronto ----------------------------------------------------------------------------- Strain: AWR73 Species: Caenorhabditis elegans Genotype: aaim-1(kea22) X. Description: aaim-1 mutants are resistant to infection by N. parisii, but sensitive to infection by Pseudomonas aeruginosa PA14. No defects in development or lifespan were observed. Reference: Tamim El Jarkass H, et al. eLife. 2022;11:e72458. PMID: 34994689. Mutagen: Outcrossed: x3 Made by: Hala Tamim El Jarkass Received: 02/04/22 University of Toronto ----------------------------------------------------------------------------- Strain: AX1295 Species: Caenorhabditis elegans Genotype: gcy-35(ok769) I. Description: Supresses aggregation and bordering phenotypes of npr-1(null) animals. Mutagen: UV/TMP Outcrossed: x5 Made by: Mario de Bono Received: 12/07/04 Institute of Science and Technology Austria, Klosterneuburg, Austria ----------------------------------------------------------------------------- Strain: AX1296 Species: Caenorhabditis elegans Genotype: gcy-36(db42) X. Description: Supresses aggregation and bordering phenotypes of npr-1(null) animals. Mutagen: EMS Outcrossed: x8 Made by: Mario de Bono Received: 12/07/04 Institute of Science and Technology Austria, Klosterneuburg, Austria ----------------------------------------------------------------------------- Strain: AX1297 Species: Caenorhabditis elegans Genotype: gcy-36(db66) X. Description: Supresses aggregation and bordering phenotypes of npr-1(null) animals. Mutagen: EMS Outcrossed: x8 Made by: Mario de Bono Received: 12/07/04 Institute of Science and Technology Austria, Klosterneuburg, Austria ----------------------------------------------------------------------------- Strain: AX1305 Species: Caenorhabditis elegans Genotype: gcy-34(ok1012) V; npr-1(ad609) X. Description: Does not supress aggregation and bordering phenotypes of npr-1(null) animals. Mutagen: Outcrossed: x Made by: Mario de Bono Received: 12/07/04 Institute of Science and Technology Austria, Klosterneuburg, Austria ----------------------------------------------------------------------------- Strain: AX1410 Species: Caenorhabditis elegans Genotype: flp-18(db99) X. Description: Impaired chemotaxis and foraging behavior. Excess intestinal fat accumulation. Reduced oxygen consumption. Derived from NL4000. Reference: Cohen M, et al. 2009 Cell Metabolism 9: 375-385. Mutagen: Outcrossed: x6 Made by: Merav Cohen Received: 03/15/25 Institute of Science and Technology Austria, Klosterneuburg, Austria ----------------------------------------------------------------------------- Strain: AX1789 Species: Caenorhabditis elegans Genotype: dbEx719. Description: dbEx719 [npr-5::mCherry + unc-122p::GFP]. Pick GFP+ to maintain. mCherry expression in a subset of amphid neurons (ADF, ASE, ASG, ASI, ASJ, ASK, AWA, AWB), in the inner labial neuron IL2, in the interneurons AIA and AUA, and in the phasmids (PHA, PHB). Expression was also seen in head, neck, and body wall muscles. Reference: Cohen M, et al. 2009 Cell Metabolism 9: 375-385. Mutagen: Outcrossed: x6 Made by: Merav Cohen Received: 03/15/25 Institute of Science and Technology Austria, Klosterneuburg, Austria ----------------------------------------------------------------------------- Strain: AX1791 Species: Caenorhabditis elegans Genotype: npr-5(ok1583) V; dbEx720. Description: dbEx720 [npr-5::npr-5(cDNA) + unc-122p::GFP]. Pick GFP+ to maintain. Intestinal fat accumulation is similar to wild-type. Reference: Cohen M, et al. 2009 Cell Metabolism 9: 375-385. Mutagen: Outcrossed: x0 Made by: Merav Cohen Received: 03/15/25 Institute of Science and Technology Austria, Klosterneuburg, Austria ----------------------------------------------------------------------------- Strain: AX1792 Species: Caenorhabditis elegans Genotype: dbEx721. Description: dbEx721 [npr-4::mCherry + unc-122p::GFP]. Pick GFP+ to maintain. mCherry expression in the AVA and RIV neurons, possibly in BAG, in the tail neuron PQR, and in the BDU neurons. Expression was also seen in the coelomocytes, the intestine, and the rectal gland cells. Reference: Cohen M, et al. 2009 Cell Metabolism 9: 375-385. Mutagen: Outcrossed: x0 Made by: Merav Cohen Received: 03/15/25 Institute of Science and Technology Austria, Klosterneuburg, Austria ----------------------------------------------------------------------------- Strain: AX2061 Species: Caenorhabditis elegans Genotype: dbEx813. Description: dbEx813 [gcy-37p::cGi500]. Pick GFP+ animals to maintain. The cGi500 cGMP sensor is expressed in the oxygen-sensing AQR, PQR, and URX neurons. Reference: Couto A, et al. Proc Natl Acad Sci U S A. 2013 Aug 27;110(35):E3301-10. Mutagen: Outcrossed: x0 Made by: Africa Couto Received: 10/27/14 Institute of Science and Technology Austria, Klosterneuburg, Austria ----------------------------------------------------------------------------- Strain: AX2157 Species: Caenorhabditis elegans Genotype: tax-2(p694) I; lin-15B&lin-15A(n765) X; dbEx722. Description: dbEx722 [flp-17p::tax-2(cDNA)::SL2::GFP + lin-15(+)]. Pick non-Muv to maintain. Array rescues tax-2 in BAG. Reference: Bretscher AJ, et al. Neuron. 2011 Mar 24;69(6):1099-113. Mutagen: Outcrossed: x0 Made by: Andrew Bretscher Received: 12/17/13 Institute of Science and Technology Austria, Klosterneuburg, Austria ----------------------------------------------------------------------------- Strain: AX2159 Species: Caenorhabditis elegans Genotype: tax-2(p694) I; lin-15B&lin-15A(n765) X; dbEx723. Description: dbEx723 [gcy-32p::tax-2(cDNA)::SL2::GFP + lin-15(+)]. Pick non-Muv to maintain. Array rescues tax-2 in AQR, PQR, and URX. Reference: Bretscher AJ, et al. Neuron. 2011 Mar 24;69(6):1099-113. Mutagen: Outcrossed: x0 Made by: Andrew Bretscher Received: 12/17/13 Institute of Science and Technology Austria, Klosterneuburg, Austria ----------------------------------------------------------------------------- Strain: AX2161 Species: Caenorhabditis elegans Genotype: tax-2(p694) I; lin-15B&lin-15A(n765) X; dbEx724. Description: dbEx724 [flp-6p::tax-2(cDNA)::SL2::GFP + lin-15(+)]. Pick non-Muv to maintain. Array rescues tax-2 in ASE using an ASE-specific flp-6p fragment. Reference: Bretscher AJ, et al. Neuron. 2011 Mar 24;69(6):1099-113. Mutagen: Outcrossed: x0 Made by: Andrew Bretscher Received: 12/17/13 Institute of Science and Technology Austria, Klosterneuburg, Austria ----------------------------------------------------------------------------- Strain: AX2164 Species: Caenorhabditis elegans Genotype: tax-2(p694) I; lin-15B&lin-15A(n765) X; dbEx725. Description: dbEx725 [gcy-8p::tax-2(cDNA)::SL2::GFP + gcy-32p::tax-2(cDNA)::SL2::GFP+ flp-17p::tax-2(cDNA)::SL2::GFP + flp-6p::tax-2(cDNA)::SL2::GFP + lin-15(+)]. Pick non-Muv to maintain. Array rescues tax-2 in AFD, AQR, PQR, URX, BAG, and ASE. Reference: Bretscher AJ, et al. Neuron. 2011 Mar 24;69(6):1099-113. Mutagen: Outcrossed: x0 Made by: Andrew Bretscher Received: 12/17/13 Institute of Science and Technology Austria, Klosterneuburg, Austria ----------------------------------------------------------------------------- Strain: AX2178 Species: Caenorhabditis elegans Genotype: tax-2(p694) I; lin-15B&lin-15A(n765) X; dbEx726. Description: dbEx726 [gcy-8p::tax-2(cDNA)::SL2::GFP + lin-15(+)]. Pick non-Muv to maintain. Array rescues tax-2 in AFD. Reference: Bretscher AJ, et al. Neuron. 2011 Mar 24;69(6):1099-113. Mutagen: Outcrossed: x0 Made by: Andrew Bretscher Received: 12/17/13 Institute of Science and Technology Austria, Klosterneuburg, Austria ----------------------------------------------------------------------------- Strain: AX301 Species: Caenorhabditis elegans Genotype: npr-1(ad609) lin-15B&lin-15A(n765) X; dbEx35. Description: dbEx35 [npr-1::GFP + lin-15(+)]. Pick Non-Muv to maintain. Solitary feeders. GFP is expressed in approximately 20 neuron types. Reference: Coates JC, de Bono M. 2002 Nature 419: 925-929. Mutagen: Outcrossed: x0 Made by: Juliet Coates Received: 10/08/13 Institute of Science and Technology Austria, Klosterneuburg, Austria ----------------------------------------------------------------------------- Strain: AX7884 Species: C. elegans Genotype: pod-2(syb1772[pod-2::His10]) II; mccc-1(syb1666[mccc-1::His10]) IV; pyc-1(syb1680[pyc-1::His10]) V; pcca-1(syb1626[pcca-1::His10]) X. Description: Superficially wild-type. Referred to as MP3-His. Strain can be used to biotinylated carboxylases from worm extracts. AX7884 obtained by crossing parental strains PHX1772 pod-2(syb1772[pod-2::His10]) II, PHX1666 mccc-1(syb1666[mccc-1::His10]) IV, PHX1680 pyc-1(syb1680[pyc-1::His10]) V and PHX1626 pcca-1(syb1626[pcca-1::His10]) X to obtain the quadruple His10-tagged strain. The 5xGlycine(G-linker)-His10 tag is a 45 bp sequence (GGAGGAGGAGGAGGACACCATCACCATCACCACCACCACCACCAC) encoding five glycine as a linker and ten histidine residues was knocked in at the C terminus-just upstream of the termination codon-of each of the four carboxylases. Reference: Artan M, et al. J Biol Chem. 2022 Aug 3:102343. doi: 10.1016/j.jbc.2022.102343. Epub ahead of print. PMID: 35933017. Mutagen: Outcrossed: x0 Made by: Murat Artan Received: 09/07/22 Institute of Science and Technology Austria, Klosterneuburg, Austria ----------------------------------------------------------------------------- Strain: AY1 Species: Caenorhabditis elegans Genotype: nol-6(ac1) II. Description: Temperature sensitive mutant. Grow at 15 to 20C. Sterile at 25C. Mutagen: EMS Outcrossed: x4 Made by: Alejandro Aballay Received: 10/06/09 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY101 Species: Caenorhabditis elegans Genotype: acIs101. Description: acIs101 [F35E12.5p::GFP + rol-6(su1006)]. Rollers. Reference: (2010) J Bio Chem 285(14):10832-40. Mutagen: Outcrossed: x4 Made by: Devin Bolz Received: 04/06/10 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY102 Species: Caenorhabditis elegans Genotype: pmk-1(km25) IV; acEx102. Description: acEx102 [vha-6p::pmk-1::GFP + rol-6(su1006)]. Maintain by picking Rollers. Reference: (2010) J Bio Chem 285(14):10832-40. Mutagen: Outcrossed: x0 Made by: Devin Bolz Received: 04/06/10 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY131 Species: C. elegans Genotype: zcIs4 V; vit-1(ac2) X. Description: zcIs4 [hsp-4::GFP] V. vit-1(ac2) is a dominant allele that causes ER stress. Since vit-1 is expressed only in adult stage, ER stress is induced only in adult stage. The levels of GFP expression from zcIs4 [hsp-4::GFP] reporter indicates the level of ER stress. Reference: Singh J & Aballay A. MBio. 2017 May 30;8(3). pii: e00778-17. doi: 10.1128/mBio.00778-17. Mutagen: EMS Outcrossed: x6 Made by: Jogender Singh Received: 06/27/19 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY132 Species: C. elegans Genotype: zcIs4 V; vit-2(ac3) X. Description: zcIs4 [hsp-4::GFP] V. vit-2(ac3) is a dominant allele that causes ER stress. Since vit-2 is expressed only in adult stage, ER stress is induced only in adult stage. The levels of GFP expression from zcIs4 [hsp-4::GFP] reporter indicates the level of ER stress. Reference: Singh J & Aballay A. MBio. 2017 May 30;8(3). pii: e00778-17. doi: 10.1128/mBio.00778-17. Mutagen: EMS Outcrossed: x6 Made by: Jogender Singh Received: 04/30/19 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY133 Species: C. elegans Genotype: zcIs4 V; vit-4(ac4) X. Description: zcIs4 [hsp-4::GFP] V. vit-4(ac4) is a dominant allele that causes ER stress. Since vit-4 is expressed only in adult stage, ER stress is induced only in adult stage. The levels of GFP expression from zcIs4 [hsp-4::GFP] reporter indicates the level of ER stress. Reference: Singh J & Aballay A. MBio. 2017 May 30;8(3). pii: e00778-17. doi: 10.1128/mBio.00778-17. Mutagen: EMS Outcrossed: x6 Made by: Jogender Singh Received: 06/27/19 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY134 Species: C. elegans Genotype: zcIs4 V; vit-5(ac5) X. Description: zcIs4 [hsp-4::GFP] V. vit-5(ac5) is a dominant allele that causes ER stress. Since vit-5 is expressed only in adult stage, ER stress is induced only in adult stage. The levels of GFP expression from zcIs4 [hsp-4::GFP] reporter indicates the level of ER stress. Reference: Singh J & Aballay A. MBio. 2017 May 30;8(3). pii: e00778-17. doi: 10.1128/mBio.00778-17. Mutagen: EMS Outcrossed: x6 Made by: Jogender Singh Received: 06/27/19 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY135 Species: C. elegans Genotype: zcIs4 V; vit-6(ac6) X. Description: zcIs4 [hsp-4::GFP] V. vit-6(ac6) is a dominant allele that causes ER stress. Since vit-6 is expressed only in adult stage, ER stress is induced only in adult stage. The levels of GFP expression from zcIs4 [hsp-4::GFP] reporter indicates the level of ER stress. Reference: Singh J & Aballay A. MBio. 2017 May 30;8(3). pii: e00778-17. doi: 10.1128/mBio.00778-17. Mutagen: EMS Outcrossed: x6 Made by: Jogender Singh Received: 06/27/19 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY142 Species: C. elegans Genotype: vit-2(ac3) X. Description: vit-2(ac3) is a dominant allele that causes ER stress. Since vit-2 is expressed only in adult stage, ER stress is induced only in adult stage. Reference: Singh J & Aballay A. MBio. 2017 May 30;8(3). pii: e00778-17. doi: 10.1128/mBio.00778-17. Mutagen: EMS Outcrossed: x6 Made by: Jogender Singh Received: 04/30/19 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY143 Species: C. elegans Genotype: flp-21(ok889) V; flp-18(gk3063) X. Description: Superficially wild-type. Reference: Singh J & Aballay A. Dev Cell. 2019 Apr 8;49(1):89-99.e4. Mutagen: Outcrossed: x0 Made by: Jogender Singh Received: 04/30/19 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY145 Species: Caenorhabditis elegans Genotype: kyIs262 IV; acEx24. Description: kyIs262 [unc-86::myr::GFP + odr-1::RFP] IV. acEx24 [srbc-48p::srbc-48(cDNA) + unc-122::GFP]. Maintain by picking GFP+ to maintain array. Integrated RFP expression in AWB and AWC. Bright GFP expression in coelomocytes. Reference: Kaur S & Aballay A. Cell Reports. 2020 May 19; 31(7): 107662. PMID: 32433971 Mutagen: EMS Outcrossed: x6 Made by: Supender Kaur Received: 10/16/20 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY146 Species: Caenorhabditis elegans Genotype: kyIs262 IV; acEx25. Description: kyIs262 [unc-86::myr::GFP + odr-1::RFP] IV. acEx25 [odr-1p::srbc-48::GFP]. Maintain by picking GFP+ to maintain array. Extra-chromosomal GFP expression in AWC neurons. Integrated RFP expression in AWB and AWC. acEx25 rescues srbc-48 mediated defects. Reference: Kaur S & Aballay A. Cell Reports. 2020 May 19; 31(7): 107662. PMID: 32433971 Mutagen: EMS Outcrossed: x6 Made by: Supender Kaur Received: 10/16/20 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY147 Species: Caenorhabditis elegans Genotype: acEx26. Description: acEx26 [odr-1p::RFP]. Pick RFP+ to maintain. RFP expression in AWC and AWB. Reference: Kaur S & Aballay A. Cell Reports. 2020 May 19; 31(7): 107662. PMID: 32433971 Mutagen: No mutagen used Outcrossed: x0 Made by: Supender Kaur Received: 10/16/20 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY156 Species: Caenorhabditis elegans Genotype: acIs156. Description: acIs156 [vit-2p::vit-2(G839R)::GFP + myo-2p::mCherry]. In contrast to VIT-2::GFP, VIT-2(G839R)::GFP remains in the intestine and is not transported to embryos. mCherry expression in pharynx. Reference: Singh J & Aballay A. MBio. 2017 May 30;8(3). pii: e00778-17. doi: 10.1128/mBio.00778-17. Mutagen: integrated by UV Outcrossed: x6 Made by: Jogender Singh Received: 11/12/19 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY157 Species: C. elegans Genotype: gon-2(q388) I; acEx157. Description: acEx157 [gon-2p::gon-2(cDNA)::SL2::GFP + unc-122::RFP]. Maintain by picking GFP+ or RFP+ animals (GFP is expressed primarily in intestine and gonads). gon-2 expression driven by gon-2 promoter. Transgene rescues gon-2(lf). Reference: Filipowicz et al. eLife 2021;10:e65935. DOI: 10.7554/eLife.65935 Mutagen: Outcrossed: x0 Made by: Jonathan Lalsiamthara Received: 09/16/21 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY158 Species: C. elegans Genotype: gon-2(q388) I; acEx158. Description: acEx158 [ges-1p::gon-2(cDNA)::SL2::GFP + unc-122::RFP]. Maintain by picking GFP+ or RFP+ animals (GFP is expressed in intestinal cells). gon-2 expression driven by ges-1 promoter. Transgene rescues gon-2(lf). Reference: Filipowicz et al. eLife 2021;10:e65935. DOI: 10.7554/eLife.65935 Mutagen: Outcrossed: x0 Made by: Jonathan Lalsiamthara Received: 09/16/21 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY159 Species: C. elegans Genotype: gtl-2(n2618) IV; acEx159. Description: acEx159 [gtl-2p::gtl-2(cDNA)::SL2::GFP + unc-122::RFP]. Maintain by picking GFP+ or RFP+ animals (GFP is expressed primarily in the excretory cell and pharynx). gtl-2 expression driven by gtl-2 promoter. Transgene rescues gtl-2(lf). Reference: Filipowicz et al. eLife 2021;10:e65935. DOI: 10.7554/eLife.65935 Mutagen: Outcrossed: x0 Made by: Jonathan Lalsiamthara Received: 09/16/21 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY160 Species: C. elegans Genotype: gtl-2(n2618) IV; acEx160. Description: acEx160 [sulp-4p::gtl-2(cDNA)::SL2::GFP + unc-122::RFP]. Maintain by picking GFP+ or RFP+ animals (GFP is expressed in the excretory cell). gtl-2 expression driven by sulp-4 promoter. Transgene rescues gtl-2(lf). Reference: Filipowicz et al. eLife 2021;10:e65935. DOI: 10.7554/eLife.65935 Mutagen: Outcrossed: x0 Made by: Jonathan Lalsiamthara Received: 09/16/21 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY161 Species: Caenorhabditis elegans Genotype: mul-1(syb1027) IV. Description: F49F1.6. mul-1(syb1027) [IV:4121342..4123166] is a CRISPR/Cas9-engineered ∼1,650-bp deletion mutant of isoforms A and B (565 bp and 952 bp deleted, with generated termination codon), leaving a predicted truncated protein of 46 amino acids. Derived by out-crossing parental strain PHX1027 (Suny Biotech) with N2 six times. Reference: Hoffman CL, et al. mBio. 2020 Mar 3;11(2):e00060-20. PMID: 32127446 Mutagen: Crispr/Cas9 Outcrossed: x6 Made by: Casandra Hoffman Received: 09/16/21 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY162 Species: Caenorhabditis elegans Genotype: mul-1(syb1027) IV; acEx162. Description: acEx162 [mul-1p::mul-1::SL2::GFP + myo-2p::mCherry]. Pick mCherry+ to maintain. GFP expression in the intestine. acEx162 transgene rescues mul-1(lf). mul-1(syb1027) [IV:4121342..4123166] is a CRISPR/Cas9 ∼1,650-bp deletion mutant of isoforms A and B (565 bp and 952 bp deleted, with generated termination codon), leaving a predicted truncated protein of 46 amino acids. Reference: Hoffman CL, et al. mBio. 2020 Mar 3;11(2):e00060-20. PMID: 32127446 Mutagen: Outcrossed: x6 Made by: Casandra Hoffman Received: 09/16/21 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY172 Species: C. elegans Genotype: mrp-1(pk89) X; acEx172. Description: acEx172 [mrp-1p::mrp-1C::SL2::GFP + unc-122p::RFP]. Pick GFP+ or RFP+ animals to maintain. mrp-1C (isoform C from cDNA) expressed under its own promoter rescues mrp-1. Reference: Lalsiamthara J & Aballay A. Commun Biol. 2022 May 5;5(1):422. doi: 10.1038/s42003-022-03381-1. PMID: 35513700. Mutagen: Outcrossed: x0 Made by: Jonathan Lalsiamthara Received: 03/29/23 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY173 Species: C. elegans Genotype: mrp-1(pk89) X; acEx173. Description: acEx173 [mrp-1::GFP]. Pick GFP+ animals to maintain. Translationally fused MRP-1::GFP expressed under its own promoter rescues mrp-1. Reference: Lalsiamthara J & Aballay A. Commun Biol. 2022 May 5;5(1):422. doi: 10.1038/s42003-022-03381-1. PMID: 35513700. Mutagen: Outcrossed: x0 Made by: Jonathan Lalsiamthara Received: 03/29/23 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY174 Species: C. elegans Genotype: mrp-1(pk89) X; acEx174. Description: acEx174 [ges-1p::mrp-1::GFP + unc-122p::RFP]. Pick GFP+ or RFP+ animals to maintain. Translational fusion of MRP-1::GFP driven by intestine-specific ges-1 promoter. Reference: Lalsiamthara J & Aballay A. Commun Biol. 2022 May 5;5(1):422. doi: 10.1038/s42003-022-03381-1. PMID: 35513700. Mutagen: Outcrossed: x0 Made by: Jonathan Lalsiamthara Received: 03/29/23 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY175 Species: C. elegans Genotype: nmr-1(ak4) II; acEx175. Description: acEx175 [glr-1p::nmr-1::SL2::GFP + unc-122p::RFP]. Pick GFP+ or RFP+ animals to maintain. glr-1 promoter drives NMR-1 expression primarily in interneuron, neurons, and ventral nerve cord. Reference: Lalsiamthara J & Aballay A. Commun Biol. 2022 May 5;5(1):422. doi: 10.1038/s42003-022-03381-1. PMID: 35513700. Mutagen: Outcrossed: x0 Made by: Jonathan Lalsiamthara Received: 03/29/23 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY176 Species: C. elegans Genotype: nmr-1(ak4) II; acEx176. Description: acEx176 [dc-1p::nmr-1::SL2::GFP + unc-122p::RFP]. Pick GFP+ or RFP+ animals to maintain. tdc-1 promoter drives NMR-1 expression primarily in RIM interneurons. Reference: Lalsiamthara J & Aballay A. Commun Biol. 2022 May 5;5(1):422. doi: 10.1038/s42003-022-03381-1. PMID: 35513700. Mutagen: Outcrossed: x0 Made by: Jonathan Lalsiamthara Received: 03/29/23 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY177 Species: C. elegans Genotype: acEx177. Description: acEx177 [ges-1p::mrp-1::GFP + vha-6::DsRed + unc-122p::RFP]. Pick RFP+ to maintain. Translationally fused MRP-1::GFP expressed under intestinal specific ges-1 promoter, MRP-1::GFP proteins localize at the basolateral membrane of the intestine. Translationally fused VHA-6::RFP expressed under its own promoter, VHA-6::RFP proteins localize at the lumen or luminal membrane of the intestine. For better results, observe fluorescence signals on the L4 stage animals and also under higher magnification microscopy. Reference: Lalsiamthara J & Aballay A. Commun Biol. 2022 May 5;5(1):422. doi: 10.1038/s42003-022-03381-1. PMID: 35513700. Mutagen: Outcrossed: x0 Made by: Jonathan Lalsiamthara Received: 03/29/23 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY178 Species: Caenorhabditis elegans Genotype: ynIs78; acEx178. Description: ynIs78 [flp-8p::GFP]. acEx178 [flp-8p::ced-3 (p15)::nz + flp-32::cz::ced-3 (p17) + unc-122p::RFP]. AUA interneurons ablated in flp-8p::GFP background. GFP-labelled AUA neurons are missing in the neuronal ablated animals. Pick RFP+ animals to maintain. Reference: Filipowicz A, et al. BMC Biol. 2022 Oct 8;20(1):229. doi: 10.1186/s12915-022-01424-x. PMID: 36209082. Mutagen: Outcrossed: x0 Made by: Jonathan Lalsiamthara Received: 04/04/23 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY179 Species: Caenorhabditis elegans Genotype: ynIs87; acEx179. Description: ynIs78 [flp-8p::GFP]. acEx179 [flp-21p::ced-3 (p15)::nz + ncs-1p::cz::ced-3 (p17) + unc-122p::RFP]. RMG interneurons ablated in flp-21p::GFP background. GFP-labelled RMG neurons are missing in the neuronal ablated animals. Pick RFP+ animals to maintain. Reference: Filipowicz A, et al. BMC Biol. 2022 Oct 8;20(1):229. doi: 10.1186/s12915-022-01424-x. PMID: 36209082. Mutagen: Outcrossed: x0 Made by: Jonathan Lalsiamthara Received: 04/04/23 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY185 Species: C. elegans Genotype: acEx185. Description: acEx185 [hsp-16.41p::par-5::VN173 + hsp-16.41p::his-1::VC155 + unc-122p::RFP]. Pick RFP+ animals to maintain. BiFC reporter strain for PAR-5 and histone (H4) proteins interaction. To detect the protein-protein physical interactions, heat shock the animals for 3 hours at 33°C, allow them to recover for 12 hours at 20°C, and observe fluorescent-complementation signals under a high-magnification fluorescence microscope. Reference: Hong C, et al. PLoS Biol. 2021 Mar 31;19(3):e3001169. doi: 10.1371/journal.pbio.3001169. PMID: 33788830. Mutagen: Outcrossed: x0 Made by: Jonathan Lalsiamthara Received: 03/29/23 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY186 Species: C. elegans Genotype: acEx186. Description: acEx186 [hsp-16.41p::par-5::VN173 + hsp-16.41p::VC155 + unc-122p::RFP]. Pick RFP+ animals to maintain. Control strain for AY185 strain. Heat shock induces protein expression but NO BiFC complementation. Reference: Hong C, et al. PLoS Biol. 2021 Mar 31;19(3):e3001169. doi: 10.1371/journal.pbio.3001169. PMID: 33788830. Mutagen: Outcrossed: x0 Made by: Jonathan Lalsiamthara Received: 03/29/23 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY187 Species: C elegans Genotype: nhr-8(ok186) IV; acEx187. Description: acEx187 [vha-6p::nhr-8::SL2::GFP + rol-6(su1006)]. Pick Rollers to maintain (GFP expression in intestine is easy to see and might be easier to score than Rol). Intestinal rescue of nhr-8(ok186) mutants. Reference: Otarigho B & Aballay A. 2020. iScience. 2020 May 22;23(5):101068. doi: 10.1016/j.isci.2020.101068. PMID: 32361270. Mutagen: Outcrossed: x0 Made by: Benson Otarigho Received: 11/15/23 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY188 Species: C elegans Genotype: unc-30(ok613) IV; acEx188. Description: acEx188 [unc-30(+) + myo-2::mCherry]. Pick mCherry+ animals to maintain. Expression of unc-30 driven by its own promoter rescues unc-30(ok613). Reference: Otarigho B & Aballay A. Cell Rep. 2021 May 25;35(8):109187. doi: 10.1016/j.celrep.2021.109187. PMID: 34038721. Mutagen: none Outcrossed: x0 Made by: Benson Otarigho Received: 11/10/23 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY189 Species: C. elegans Genotype: unc-30(ok613) IV; acEx189. Description: acEx189 [rab-3p::unc-30 + myo-2::mCherry]. Pick mCherry+ animals to maintain. Expression of unc-30 driven by rab-3 neuronal promoter rescues unc-30(ok613) in neurons. Reference: Otarigho B & Aballay A. Cell Rep. 2021 May 25;35(8):109187. doi: 10.1016/j.celrep.2021.109187. PMID: 34038721. Mutagen: N/A Outcrossed: x0 Made by: Benson Otarigho Received: 11/10/23 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY190 Species: C. elegans Genotype: acEx190. Description: acEx190 [tax-2p::CZ::ced-3(p17)::unc-54 3’UTR + lim-6p::ced-3(p15)::NZ::unc-54 3’UTR + myo-3p::mCherry]. Pick mCherry+ to maintain.  ASG is ablated in animals carrying the array by employing a two-component system reconstituted caspase (recCaspase) using the tax-2 and lim-6 promoters.  Strain viable at all temperatures.  Reference Otarigho, B. and Aballay, A., 202. Cell reports, 35(8). PMID: 34038721. Mutagen: N/A Outcrossed: x0 Made by: Benson Otarigho Received: 11/29/23 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AY191 Species: C elegans Genotype: acEx191. Description: acEx191 [odr-2p::CZ::ced-3(p17)::unc-54 3’UTR+ unc-53p::ced-3(p15)::NZ::unc-54 3’UTR + rol-6(su1006)]. Pick Rollers to maintain.  PVP is ablated in animals carrying the array by employing a two-component system reconstituted caspase (recCaspase) using the odr-2 and unc-53 promoters.  Strain viable at all temperatures.  Reference Otarigho, B. and Aballay, A., 202. Cell reports, 35(8). PMID: 34038721. Mutagen: N/A Outcrossed: x0 Made by: Benson Otarigho Received: 11/29/23 MD Anderson Cancer Center, Houston, TX ----------------------------------------------------------------------------- Strain: AZ212 Species: Caenorhabditis elegans Genotype: unc-119(ed3) ruIs32 III. Description: ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. Homozygous expression of GFP::H2B histone fusion in germline. pAZ132. Mutagen: Microparticle Bombardment Outcrossed: x0 Made by: Vida Praitis Received: 05/07/01 University of Chicago, IL ----------------------------------------------------------------------------- Strain: AZ217 Species: Caenorhabditis elegans Genotype: unc-119(ed3) ruIs37 III. Description: ruIs37 [myo-2p::GFP + unc-119(+)] III. Expresses GFP in the pharynx. pAZ119. Mutagen: Microparticle Bombardment Outcrossed: x0 Made by: Vida Praitis Received: 05/17/01 University of Chicago, IL ----------------------------------------------------------------------------- Strain: AZ218 Species: Caenorhabditis elegans Genotype: unc-119(ed3) ruIs38 III. Description: ruIs38 [partial myo-2 promoter::GFP + unc-119(+)]. Expresses GFP in the pharynx. pAZ119. Mutagen: Microparticle Bombardment Outcrossed: x0 Made by: Vida Praitis Received: 05/17/01 University of Chicago, IL ----------------------------------------------------------------------------- Strain: AZ235 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; ruIs48. Description: ruIs48 [pie-1::tubulin::GFP + unc-119(+)]. Homozygous expression of GFP::tubulin fusion in germline and early embryos. Insertion unmapped. pAZ147. Mutagen: Microparticle Bombardment Outcrossed: x0 Made by: Vida Praitis Received: 05/07/01 University of Chicago, IL ----------------------------------------------------------------------------- Strain: AZ244 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; ruIs57. Description: ruIs57 [pie-1p::GFP::tubulin + unc-119(+)]. Homozygous expression of GFP::tubulin fusion in germline and early embryos. Insertion unmapped. pAZ147. Mutagen: Microparticle Bombardment Outcrossed: x0 Made by: Vida Praitis Received: 05/07/01 University of Chicago, IL ----------------------------------------------------------------------------- Strain: AZ30 Species: Caenorhabditis elegans Genotype: sma-1(ru18) V. Description: Strong Sma phenotype. Null allele.  NOTE: Some animals Roll Mutagen: UV/Psoralen Outcrossed: x3 Made by: Vida Praitis Received: 05/07/01 University of Chicago, IL ----------------------------------------------------------------------------- Strain: BA1 Species: Caenorhabditis elegans Genotype: fer-1(hc1) I. Description: Temperature sensitive. M-MATING+LOW TEMP ONLY. Recessive. Fertilization abnormal. Mutagen: EMS Outcrossed: x Made by: Ward S Received: 03/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: BA1013 Species: Caenorhabditis elegans Genotype: spe-6(hc49) vab-7(e1562)/qC1 [dpy-19(e1259) glp-1(q339)] III; spe-27(it132) IV. Description: Male/hermaphrodite line. Maintain at 15C to insure maintenance of male/hermaphrodite line. Mutagen: Outcrossed: x Made by: Paul Muhlrad Received: 02/28/05 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA1044 Species: Caenorhabditis elegans Genotype: spe-27(it132) spe-29(it127) IV; him-5(e1490) V. Description: Non-conditional hermaphrodite sterile. Males fertile. Self-progeny produced after mating. Mutagen: Outcrossed: x Made by: Jeremy Nance Received: 01/05/01 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA1051 Species: Caenorhabditis elegans Genotype: spe-12(hc76) I; spe-29(it127) IV; him-5(e1490) V. Description: Self-sterile hermaphrodites; fertile males. Mated hermaphrodites produce self and outcross progeny. Self progeny are 33% males. Mutagen: Outcrossed: x Made by: Jeremy Nance Received: 01/05/01 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA1061 Species: Caenorhabditis elegans Genotype: dpy-18(e364) spe-6(hc49) ale-1(mc14)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, Sterile Dpys (Dpy is temperature sensitive), and dead eggs. ale-1 is a recessive embryonic lethal. Mutagen: Outcrossed: x Made by: Paul Muhlrad Received: 02/28/05 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA1069 Species: Caenorhabditis elegans Genotype: F26F4.8(hc180) III. Description: Deletion allele of F26F4.8. Homologous to Zn finger of Drosophila ovo transcription factor. Developmental defects in hind gut, germline and vulva. HES primers 106/107 outer and HES 108/109 inner. Mutagen: UV/TMP Outcrossed: x6 Made by: H. Smith Received: 10/15/01 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA1070 Species: Caenorhabditis elegans Genotype: cdh-5(hc181) IV. Description: Deletion allele of F08B4.2. Homologous to Drosophila fat cadherin. Primers HES 114/115 outer and HES 116/117 inner. Mutagen: UV/TMP Outcrossed: x2 Made by: H. Smith Received: 10/15/01 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA1073 Species: Caenorhabditis elegans Genotype: dyf-5(hc183) I. Description: Deletion allele of M04C9.5 (mak-kinase). No visible phenotype in hermaphrodites. Primers HES 186/187 outer and HES 188/189 inner. Mutagen: Outcrossed: x0 Made by: H. Smith Received: 10/15/01 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA1083 Species: Caenorhabditis elegans Genotype: F43G6.6(hc184) II. Description: Deletion allele of F43G6.6. No obvious phenotype in hermaphrodites. HES primers 162/163 outer and 164/165 inner. Mutagen: UV/TMP Outcrossed: x0 Made by: H. Smith Received: 10/15/01 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA1084 Species: Caenorhabditis elegans Genotype: F09C12.2(hc185) II. Description: Deletion allele of F09C12.2. No obvious phenotype in hermaphrodites. HES primers 122/123 outer and HES 124/125 inner. Mutagen: UV/TMP Outcrossed: x0 Made by: H. Smith Received: 10/15/01 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA1090 Species: Caenorhabditis elegans Genotype: cav-2(hc191) V. Description: No visible phenotype in hermaphrodites. Deletion allele of C56A3.7. Mutagen: TMP Outcrossed: x6 Made by: HES Received: 07/26/01 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA1091 Species: Caenorhabditis elegans Genotype: C35D10.2(hc192) III. Description: No visible phenotype in hermaphrodites. Primes HES 146/146 and HES 148/149. Mutagen: TMP Outcrossed: x6 Made by: HES Received: 10/23/01 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA1093 Species: Caenorhabditis elegans Genotype: snf-10(hc194) V. Description: Y32F6A.2 No visible phenotype in hermaphrodites. Primers: outer HES 22/228 and inner HES 229/230. Mutagen: TMP Outcrossed: x6 Made by: H. Smith Received: 10/15/01 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA14 Species: Caenorhabditis elegans Genotype: fer-14(hc14) X. Description: Temperature sensitive. 8% of total oocytes produced are fertilized and give rise to larvae at 16C. Almost completely sterile at 25C. Mutagen: EMS Outcrossed: x2 Made by: Tom Roberts Received: 11/01/84 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA15 Species: Caenorhabditis elegans Genotype: rrf-3(hc15) II. Description: Temperature sensitive. Maintain at 15C. Mutagen: EMS Outcrossed: x2 Made by: Tom Roberts Received: 11/01/84 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA17 Species: Caenorhabditis elegans Genotype: fem-1(hc17) IV. Description: Temperature sensitive. Recessive. Feminization. Mutagen: EMS Outcrossed: x Made by: Nelson G Received: 03/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: BA2 Species: Caenorhabditis elegans Genotype: fer-2(hc2) III. Description: Temperature sensitive. Maintain at 15C. Some growth at 20C. Does not grow at 25C. Recessive. Mutagen: EMS Outcrossed: x Made by: Ward S Received: 03/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: BA23 Species: Caenorhabditis elegans Genotype: fer-6(hc23) I. Description: Temperature sensitive. Fertilization abnormal. Recessive. Mutagen: EMS Outcrossed: x Made by: Ward S Received: 03/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: BA24 Species: Caenorhabditis elegans Genotype: fer-1(hc24) I. Description: Fertilization abnormal. Recessive. Leaky temperature sensitive. Mutagen: EMS Outcrossed: x Made by: Ward S Received: 03/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: BA3 Species: Caenorhabditis elegans Genotype: eri-3(hc3) II. Description: Temperature sensitive. Fertilization abnormal. Recessive. Mutagen: EMS Outcrossed: x Made by: Ward S Received: 03/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: BA34 Species: Caenorhabditis elegans Genotype: fer-7(hc34) I. Description: Fertilization abnormal. Recessive. Temperature sensitive. Maintain at 15C. Very leaky: grows at 20C and also somewhat at 25.4C to 25.8C. Mutagen: EMS Outcrossed: x Made by: Ward S Received: 03/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: BA4 Species: Caenorhabditis elegans Genotype: fer-4(hc4) V. Description: Fertilization abnormal. Recessive. Temperature sensitive. Maintain at 15C. Some growth at 20C and 25.4 C. Mutagen: EMS Outcrossed: x Made by: Ward S Received: 03/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: BA521 Species: Caenorhabditis elegans Genotype: fer-1(hc1) unc-29(e1072) I. Description: Temperature sensitive. Maintain at 15C. Unc. Mutagen: Outcrossed: x Made by: Received: 02/01/86 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA522 Species: Caenorhabditis elegans Genotype: fer-1(hc13) unc-29(e1072) I. Description: Temperature sensitive. Maintain at 15C. Unc. Mutagen: EMS Outcrossed: x Made by: Received: 02/01/86 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA524 Species: Caenorhabditis elegans Genotype: fer-1(hc1) I; him-5(e1490) V. Description: Temperature sensitive. Maintain at 15C. Throws males. Mutagen: Outcrossed: x Made by: Y. Argon Received: 11/01/84 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA547 Species: Caenorhabditis elegans Genotype: fer-2(hc2) III; him-5(e1490) V. Description: Fertile at 16c and 20C. Sterile at 25C-Produces some defective embryos and 0-5 progeny. Throws males. Mutagen: Outcrossed: x Made by: Received: 11/01/84 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA562 Species: Caenorhabditis elegans Genotype: fer-4(hc4) him-5(e1490) V. Description: Temperature sensitive. Maintain at 15C. Produces about 30% males. Mutagen: Outcrossed: x Made by: Steve L'Hernault Received: 11/01/84 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA585 Species: Caenorhabditis elegans Genotype: spe-10(hc104) unc-76(e911) V. Description: Unc. Temperature sensitive, maintain at 16C. Average progeny: at 16C= 7 +/- 5; at 25C= 0.1 +/- .1. Mutagen: EMS Outcrossed: x Made by: Steve L'Hernault Received: 02/03/93 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA590 Species: Caenorhabditis elegans Genotype: spe-10(hc104) dpy-11(e224) V. Description: Dpy. Temperature sensitive, maintain at 16C. Average progeny at 16C= 7 +/- 5. At 25C, average progeny = 0.1 =/- .1. Mutagen: EMS Outcrossed: x Made by: Steve L'Hernault Received: 02/03/93 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA6 Species: Caenorhabditis elegans Genotype: fer-6(hc6) I. Description: Fertilization abnormal. Temperature sensitive. Recessive. Mutagen: EMS Outcrossed: x Made by: Ward S Received: 03/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: BA606 Species: Caenorhabditis elegans Genotype: spe-6(hc49) unc-25(e156) III; eDp6 (III;f). Description: Animals with the Duplication are WT. Animals without the Duplication are Unc and Sterile. Mutagen: EMS Outcrossed: x10 Made by: Steve L'Hernault Received: 02/03/93 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA609 Species: Caenorhabditis elegans Genotype: spe-6(hc49) vab-7(e1562) III; eDp6 (III;f). Description: Animals with the Duplications are WT. Animals which have lost the Duplication are Sterile and have an abnormal tail. Mutagen: EMS Outcrossed: x10 Made by: Steve L'Hernault Received: 02/03/93 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA671 Species: Caenorhabditis elegans Genotype: spe-9(hc88) I. Description: Self-sterile at 25C. Maintain at 15C. Mutagen: EMS Outcrossed: x>2 Made by: Diane Shakes Received: 11/01/94 Emory University, Atlanta, GA ----------------------------------------------------------------------------- Strain: BA676 Species: Caenorhabditis elegans Genotype: spe-6(hc92) unc-32(e189) III; eDp6 (III;f). Description: Unc. Animals which have lost the duplications are Sterile and Unc. Mutagen: EMS Outcrossed: x15 Made by: Diane Shakes Received: 01/11/93 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA708 Species: Caenorhabditis elegans Genotype: spe-9(hc52) I. Description: Maintain at 15C. Mutagen: EMS Outcrossed: x Made by: Received: 06/15/88 Emory University, Atlanta, GA ----------------------------------------------------------------------------- Strain: BA709 Species: Caenorhabditis elegans Genotype: fer-1(hc80) unc-29(e1072)/sDf6 I. Description: Heterozygotes are WT and segregate WT heterozygotes, Sterile Unc (fer-1 unc-29 homozygotes) and L1 lethals (sDf6 homozygotes). hc80 is a nonconditional sterile. hc80 sperm have short pseudopods. Mutagen: EMS Outcrossed: x>3 Made by: Steve L'Hernault Received: 10/17/94 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA714 Species: Caenorhabditis elegans Genotype: sDf5/spe-4(hc78) I. Description: Heterozygotes are WT and segregate WT heterozygotes, Sterile spe-4 homozygotes, and dead eggs (sDf5 homozygotes). Mutagen: EMS Outcrossed: x Made by: Steve L'Hernault Received: 06/15/88 Emory University, Atlanta, GA ----------------------------------------------------------------------------- Strain: BA717 Species: Caenorhabditis elegans Genotype: spe-11(hc90) I; sDp2 (I;f). Description: Animals carrying the duplication WT. Animals which have lost the Dup are sterile. Maintain by picking fertile animals and checking for segregation of fertile and sterile progeny. Mutagen: EMS Outcrossed: x Made by: Received: 06/15/88 Emory University, Atlanta, GA ----------------------------------------------------------------------------- Strain: BA744 Species: Caenorhabditis elegans Genotype: spe-10(hc104) V. Description: Temperature sensitive, maintain at 15C. Average progeny at 16C is 7 +/- 5. Average progeny at 25C is 0.1 +/- .1. Mutagen: EMS Outcrossed: x Made by: Diane Shakes Received: 02/03/93 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA759 Species: Caenorhabditis elegans Genotype: hcDf1 IV; eEx25. Description: eEx25 [C13G4(cosmid) + XDM23(phage)]. Animals with eEx25 are WT. Pick wild-type to maintain. Animals which have lost eEx25 are Twitchers and Sterile (occasionally produce young). This cosmid lacks the 3' end of the unc-22 gene while XDM23 lacks the 5' end of unc-22, but following injection an extrachromosomal array was formed that included at least one functional unc-22 gene. Mutagen: Spontaneous in TR466 Outcrossed: x Made by: Steve L'Hernault Received: 11/01/94 Emory University, Atlanta, GA ----------------------------------------------------------------------------- Strain: BA763 Species: Caenorhabditis elegans Genotype: spe-5(hc93) I; sDp2 (I;f). Description: Animals with the Duplications are WT. Animals which have lost the Duplication are Sterile. Mutagen: EMS Outcrossed: x Made by: Received: 06/15/88 Emory University, Atlanta, GA ----------------------------------------------------------------------------- Strain: BA782 Species: Caenorhabditis elegans Genotype: spe-10(hc104) him-5(e1490) V. Description: Temperature sensitive, maintain at 16C. Segregates males. Average progeny at 16C is 7 +/- 5. Average progeny at 25C is 0.1 +/- .1. Mutagen: EMS Outcrossed: x Made by: Diane Shakes. Received: 02/03/93 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA783 Species: Caenorhabditis elegans Genotype: spe-12(hc76) I. Description: Maintain by mating. Hermaphrodites produce nonfunctional sperm and are fertilization defective. Self-fertility is <1% at 16C or 25C. Males are fertile. Mutagen: EMS Outcrossed: x Made by: Steve L'Hernault Received: 06/15/88 Emory University, Atlanta, GA ----------------------------------------------------------------------------- Strain: BA784 Species: Caenorhabditis elegans Genotype: spe-8(hc50) I. Description: Male-hermaphrodite mating strain. Males are fertile, hermaphrodites are sterile. Mutagen: Steve L'Hernault Outcrossed: x Made by: EMS Received: 01/05/01 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA785 Species: Caenorhabditis elegans Genotype: spe-8(hc40) I. Description: Hermaphrodites produce nonfunctional, nonmotile sperm with uniformly aberrant pseudopods. Male sperm normal. Self-fertility is <1% at 16C or 25C. Maintain by mating. Mutagen: EMS Outcrossed: x Made by: Received: 06/15/88 Emory University, Atlanta, GA ----------------------------------------------------------------------------- Strain: BA786 Species: Caenorhabditis elegans Genotype: spe-8(hc53) I. Description: Male-hermaphrodite mating strain. Males are fertile, hermaphrodites are sterile. Mutagen: Steve L'Hernault Outcrossed: x Made by: EMS Received: 01/05/01 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA793 Species: Caenorhabditis elegans Genotype: spe-26(hc138) dpy-13(e184) IV. Description: Temperature sensitive. Fertile at 16C. Partially fertile at 20C. Sterile at 25C. Spermatogenesis arrests at the spermatocyte stage. Mutagen: EMS Outcrossed: x15 Made by: Jacob Varkey Received: 02/03/93 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA794 Species: Caenorhabditis elegans Genotype: spe-13(hc137) I. Description: Temperature sensitive. Maintain at 15C. Mutagen: EMS Outcrossed: x Made by: Received: 06/15/88 Emory University, Atlanta, GA ----------------------------------------------------------------------------- Strain: BA811 Species: Caenorhabditis elegans Genotype: sDf5/spe-4(hc78) unc-15(e73) I. Description: Heterozygotes are WT and segregate WT heterozygotes, Sterile Unc spe-4 unc-15 homozygotes, and dead eggs (sDf5 homozygotes). Pick wild-type to maintain. Mutagen: EMS Outcrossed: x Made by: Steve L'Hernault Received: 06/15/88 Emory University, Atlanta, GA ----------------------------------------------------------------------------- Strain: BA819 Species: Caenorhabditis elegans Genotype: spe-11(hc77) I. Description: Temperature sensitive sterile. Paternal effect embryonic lethal. Permissive temp is 20C. Restrictive temp is 25C. Mutagen: Outcrossed: x>4 Made by: Diane Shakes Received: 10/17/94 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA821 Species: Caenorhabditis elegans Genotype: spe-26(hc138) IV. Description: Temperature-sensitive. Maintain at 15C. Fertile at 15C. Partially fertile at 20C. Sterile at 25C. Spermatogenesis arrests at the spermatocyte stage. Increase lifespan in males and hermaphrodites. Mutagen: EMS Outcrossed: x15 Made by: Jacob Varkey Received: 01/11/93 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA824 Species: Caenorhabditis elegans Genotype: spe-26(hc139) dpy-20(e1282) IV. Description: Temperature sensitive. Partially fertile at 16C (very few progeny). Sterile at 20C and 25C. Weak Dpy at 15C. Spermatogenesis arrests at the spermatocyte stage. Mutagen: EMS Outcrossed: x15 Made by: Jacob Varkey Received: 02/03/93 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA825 Species: Caenorhabditis elegans Genotype: spe-26(hc140) dpy-20(e1282)/+ IV. Description: Heterozygotes are WT and segregate wild-type, wild-type heterozygotes, and Sterile Dpy (spe-4 dpy-20 homozygotes). Homozygous mutants are weak Dpy and partially fertile at 15C. Sterile at 20-25C. Spermatogenesis arrests at the spermatocyte stage. Mutagen: EMS Outcrossed: x15 Made by: Jacob Varkey Received: 02/03/93 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA836 Species: Caenorhabditis elegans Genotype: spe-26(it112) unc-22(e66) IV. Description: Temperature-sensitive. Fertile at 15C. Partially fertile at 20C. Sterile at 25C. Twitcher. Spermatogenesis arrests at the spermatocyte stage. Mutagen: EMS Outcrossed: x10 Made by: Diane Shakes Received: 01/11/93 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA837 Species: Caenorhabditis elegans Genotype: spe-26(it112) IV. Description: Temperature-sensitive. Fertile at 15C. Partially fertile at 20C. Sterile at 25C. Spermatogenesis arrests at the spermatocyte stage. Mutagen: EMS Outcrossed: x10 Made by: Diane Shakes Received: 01/11/93 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA838 Species: Caenorhabditis elegans Genotype: spe-26(hc140) IV. Description: Temperature sensitive. Weak Dpy and partial fertility at 15C (very few progeny). Sterile at 20C and 25C. Spermatogenesis arrests at the spermatocyte stage. Mutagen: EMS Outcrossed: x15 Made by: Jacob Varkey Received: 02/03/93 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA839 Species: Caenorhabditis elegans Genotype: spe-26(it118) unc-22(e66) IV. Description: Temperature-sensitive. Fertile at 15C. Partially fertile at 20C. Sterile at 25C. Twitcher. Spermatogenesis arrests at the spermatocyte stage. Mutagen: EMS Outcrossed: x10 Made by: Diane Shakes Received: 01/11/93 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA840 Species: Caenorhabditis elegans Genotype: spe-26(hc139) IV. Description: Temperature sensitive. Few progeny at 16C. Sterile at 20C and 25C. Spermatogenesis arrests at the spermatocyte stage. Mutagen: EMS Outcrossed: x15 Made by: Jacob Varkey Received: 02/03/93 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA843 Species: Caenorhabditis elegans Genotype: spe-26(it118) IV. Description: Temperature-sensitive. Fertile at 15C. Partially fertile at 20C. Sterile at 25C. Spermatogenesis arrests at the spermatocyte stage. Mutagen: EMS Outcrossed: x10 Made by: Diane Shakes Received: 02/03/93 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA883 Species: Caenorhabditis elegans Genotype: spe-12(hc152) I. Description: Male-hermaphrodite mating strain. Males are fertile, hermaphrodites are sterile. Mutagen: Outcrossed: x Made by: Received: 01/05/01 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA900 Species: Caenorhabditis elegans Genotype: spe-27(it110) IV. Description: Male/hermaphrodite mating strain. Hermaphrodites self-sterile at all temps, males fertile. Spermatids activate "normally" in TEA. In pronase they produce spikes but never form pseudopods. Similar to spe-12 and spe-8. Mutagen: EMS Outcrossed: x Made by: Diane Shakes Received: 08/16/96 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA901 Species: Caenorhabditis elegans Genotype: spe-27(it110) dpy-20(e1282) IV. Description: Temperature sensitive Dpy. Male/hermaphrodite mating strain. Hermaphrodites self-sterile at all temps; males fertile. Spermatids arrest with spikes in pronase; form normal pseudopods in TEA. Mutagen: Outcrossed: x Made by: Received: 08/16/96 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA925 Species: Caenorhabditis elegans Genotype: spe-26(hc138) dpy-20(e1282) IV. Description: Temperature-sensitive. Fertile and weak Dpy at 15C. Partially fertile at 20C. Sterile at 25C. Twitcher. Spermatogenesis arrests at the spermatocyte stage. Mutagen: EMS Outcrossed: x15 Made by: Jacob Varkey Received: 02/03/93 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA947 Species: Caenorhabditis elegans Genotype: spe-27(hc161) IV. Description: Male/hermaphrodite mating strain. Hermaphrodites self-sterile at all temps; males fertile. Mutagen: EMS Outcrossed: x6 Made by: Alicia Minniti Received: 08/16/96 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA959 Species: Caenorhabditis elegans Genotype: spe-29(it127) dpy-20(e1282) IV. Description: Homozygous male/hermaphrodite line. Males are fertile. Hermaphrodites are sterile, but slightly leaky producing a few progeny (at 25C - ts not tested). In pronase, spermatids from males activate to form many long spikes, terminating at this stage. A very few (1-3 per worm) activate to form normal-looking, motile spermatozoons. Mutagen: Outcrossed: x Made by: Received: 01/05/01 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA962 Species: Caenorhabditis elegans Genotype: spe-29(it127) IV. Description: Hermaphrodites are sterile; Males are fertile. Hermaphrodites lay oocytes (produce a few fertile eggs and many oocytes). Produce viable progeny when mated to males. Hermaphrodites produce 10X more self progeny at 20C (2.5/herm) than at either 16C or 25C. Mutagen: Outcrossed: x3 Made by: Jeremy Nance Received: 01/05/01 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA963 Species: Caenorhabditis elegans Genotype: spe-27(it132) IV. Description: Temperature sensitive spe-27 allele. Hermaphrodites are sterile at 25C but produce 30-40 progeny/hermaphrodite at 16C. Males are fertile. Mutagen: EMS Outcrossed: x Made by: Received: 08/16/96 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA966 Species: Caenorhabditis elegans Genotype: spe-27(it132) unc-22(e66) IV. Description: Temperature sensitive spe-27 allele. Hermaphrodites sterile at 25C; hermaphrodites produce 30-40 progeny/hermaphrodite at 16C. Males cannot mate due to the unc-22 mutation. Maintain at 15C. Mutagen: Outcrossed: x Made by: Paul Muhlrad Received: 08/16/96 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA968 Species: Caenorhabditis elegans Genotype: spe-29(it127) unc-24(e138) IV. Description: Unc. Leaky sterile at 20C; tighter at 16C and 25C. Easier to propagate as if male-female strain. Mutagen: Outcrossed: x Made by: Jeremy Nance Received: 01/05/01 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA969 Species: Caenorhabditis elegans Genotype: spe-6(hc163) III; spe-27(it132) dpy-20(e1282) IV. Description: Dpy. spe-6(hc163) is a recessive suppressor of spe-27(it132). spe-6(hc163) also suppresses spe-8, spe-12, spe-29 and other spe-27 alleles. Causes precocious spermatid activation. Fertile between 15-25C. Mutagen: EMS (hc163) Outcrossed: x5 Made by: Paul Muhlrad Received: 07/29/03 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA970 Species: Caenorhabditis elegans Genotype: spe-6(hc163) III; spe-27(it132) unc-22(e66) IV. Description: Twitcher Unc. spe-6(hc163) is a recessive suppressor of spe-27(it132). spe-6(hc163) also suppresses spe-8, spe-12, spe-29 and other spe-27 alleles. Causes precocious spermatid activation. Fertile between 15-25C. Mutagen: EMS (hc163) Outcrossed: x4 Made by: Paul Muhlrad Received: 07/29/03 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA971 Species: Caenorhabditis elegans Genotype: spe-27(it132) dpy-20(e1282) IV. Description: Temperature sensitive spe-27 allele. Leaky sterile at 20C. Males at 20C produce spermatids that form spikes in pronase. Males are fertile. Temperature sensitive dpy-20 allele. Maintain at 15C. Mutagen: Outcrossed: x Made by: Received: 08/16/96 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA975 Species: Caenorhabditis elegans Genotype: spe-6(hc163) III; spe-29(it127) dpy-20(e1282) IV. Description: spe-6(hc163) suppresses the self-sterility of spe-29 in this strain. Self-sterile at 25C. Mutagen: Outcrossed: x Made by: Paul Muhlrad Received: 02/28/05 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA979 Species: Caenorhabditis elegans Genotype: dpy-5(e61) spe-12(hc76) I; spe-6(hc163) III. Description: spe-6(hc163) suppresses hermaphrodite self-sterility of hc76. Dpy. Mutagen: Outcrossed: x Made by: Paul Muhlrad Received: 02/28/05 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA984 Species: Caenorhabditis elegans Genotype: spe-6(hc163) dpy-18(e364) III. Description: Dpy. spe-6(hc163) suppresses self-sterility of spe-8, spe-12, spe-27, and spe-29 mutants. Causes precocious spermatide activation. Fertile between 15-25C. Mutagen: EMS (hc163) Outcrossed: x Made by: Paul Muhlrad Received: 07/29/03 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BA989 Species: Caenorhabditis elegans Genotype: spe-6(hc163) III; spe-27(it132) IV. Description: spe-6(hc163) suppresses the ts self-sterile phenotype of spe-27(it132). Also suppresses spe-8, spe-12, and spe-29. Causes precocious spermatid activation. Lays eggs and oocytes. Males are weakly fertile. Fertile between 15-25C. Mutagen: EMS (hc163) Outcrossed: x Made by: Paul Muhlrad Received: 07/29/03 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: BAY7 Species: C. elegans Genotype: yanSi1 II; unc-119(ed3) III. Description: yanSi1 [eft-3p::dCAS9::VP64::tbb-2 3'UTR + Cbr-unc-119(+)] inserted into ttTi5605 (II:8.42 MB) in parental strain EG6699. Integration plasmid was generated via 3-way gateway reaction with pCFJ150 and plasmids containing eft-3 promoter (ID:1031@E02 promoterome), tbb-2 3'UTR (pCM1.36), and a full length dcas9::VP64 transgene cloned into pDONR201. Reference: Zullo JM, et al. Nature. 2019 Oct;574(7778):359-364. doi: 10.1038/s41586-019-1647-8. PMID: 31619788. Mutagen: Outcrossed: x0 Made by: Joe Zullo Received: 08/27/24 Harvard Medical School, Boston, MA ----------------------------------------------------------------------------- Strain: BB1 Species: Caenorhabditis elegans Genotype: dcr-1(ok247)/unc-32(e189) III. Description: Heterozygotes are WT and segregate WT heterozygote, Uncs (unc-32 homozygotes), and Steriles (dcr-1 homozygotes). Mutagen: EMS Outcrossed: x8 Made by: B. Barstead Received: 01/30/02 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: BB19 Species: Caenorhabditis elegans Genotype: adr-1(tm668) I. Description: Reduced lifespan, chemotaxis defective, co-suppression of transgenes in somatic cells. Maintain under normal conditions. Reference: Hundley HA, et al. RNA. 2008 Oct;14(10):2050-60. Mutagen: TMP/UV Outcrossed: x8 Made by: Mitani / Bass Received: 03/01/12 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: BB2 Species: Caenorhabditis elegans Genotype: adr-1(gv6) I. Description: Defective chemotaxis to volatile odorant. Low percentage (about 6%) have protruding vulva. Mutagen: EMS Outcrossed: x8 Made by: Michael Krause Received: 02/05/03 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: BB21 Species: Caenorhabditis elegans Genotype: adr-1(tm668) I; adr-2(ok735) III. Description: Reduced lifespan, chemotaxis defective, co-suppression of transgenes in somatic cells. Maintain under normal conditions. Reference: Hundley HA, et al. RNA. 2008 Oct;14(10):2050-60. Mutagen: EMS Outcrossed: x8 Made by: Brenda Bass Received: 03/01/12 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: BB22 Species: Caenorhabditis elegans Genotype: adr-2(gv42) rde-4(ne299) III. Description: RNAi deficient. Maintain under normal conditions. Mutagen: EMS Outcrossed: x8 Made by: Brenda Bass Received: 03/01/12 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: BB23 Species: Caenorhabditis elegans Genotype: adr-1(gv6) I; adr-2(gv42) III; rde-1(ne219) V. Description: RNAi deficient. Maintain under normal conditions. Reference: Tonkin LA & BassBL. Science. 2003 Dec 5;302(5651):1725. Knight SW & Bass BL. Mol Cell. 2002 Oct;10(4):809-17. Mutagen: EMS Outcrossed: x8 Made by: Brenda Bass Received: 03/01/12 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: BB239 Species: Caenorhabditis elegans Genotype: adr-1(uu49) I; adr-2(uu28) III. Description: Chemotaxis deficient. Transgenes are silenced in this background. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. NOTE: In the referenced publication, irregularities were noted in the adr-1(gv6);adr-2(gv42) null strain, which were ascribed to background mutationsint hat strain. This strain -- adr-1(uu49);adr-2(uu28) -- was generated Crispr/Cas9 targeted mutation and phenotypes are more consistent with another null strain, adr-1(tm668);adr-2(ok735). Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Daniel Reich Received: 03/26/18 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: BB24 Species: Caenorhabditis elegans Genotype: adr-1(gv6) I; adr-2(gv42) rde-4(ne299) III. Description: RNAi deficient. Maintain under normal conditions. Reference: Tonkin LA & BassBL. Science. 2003 Dec 5;302(5651):1725. Mutagen: EMS Outcrossed: x8 Made by: Brenda Bass Received: 03/01/12 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: BB242 Species: Caenorhabditis elegans Genotype: adr-1(uu49) I; adr-2(uu28) III; rde-1(uu51) V. Description: RNAi deficient. Lacks RNA editing. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Daniel Reich Received: 03/26/18 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: BB244 Species: Caenorhabditis elegans Genotype: adr-1(uu49) I; adr-2(uu28) rde-4(uu53) III. Description: RNAi deficient. Lacks RNA editing. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Daniel Reich Received: 03/26/18 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: BB259 Species: Caenorhabditis elegans Genotype: adr-1(uu49) I; adr-2(uu28) III; ggIs1 IV. Description: ggIs1 [nrde-3p::3xFlag::GFP::nrde-3 ORF + unc-119(+)] IV. Nuclear GFP::NRDE-3 signal. Lacks RNA editing. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. Mutagen: Outcrossed: x0 Made by: Daniel Reich Received: 03/26/18 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: BB261 Species: Caenorhabditis elegans Genotype: adr-1(uu49) I; rrf-3(uu56) II; adr-2(uu28) III. Description: Enhanced RNAi. Lacks RNA editing. Vulval bursting. Low brood size. Triple mutants display bursting and low brood size phenotypes not observed in adr-1;adr-2 or rrf-3 parental strains. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Daniel Reich Received: 03/26/18 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: BB270 Species: Caenorhabditis elegans Genotype: adr-1(uu49) I; rrf-3(uu56) II; adr-2(uu28) III; rde-1(uu51) V. Description: RNAi deficient. Lacks RNA editing. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Daniel Reich Received: 03/26/18 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: BB272 Species: Caenorhabditis elegans Genotype: adr-1(uu49) I; rrf-3(uu56) II; adr-2(uu28) rde-4(uu53) III. Description: RNAi deficient. Lacks RNA editing. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Daniel Reich Received: 03/26/18 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: BB278 Species: Caenorhabditis elegans Genotype: adr-1(uu49) I; rrf-3(uu56) II; adr-2(uu28) III; ggIs1 IV. Description: ggIs1 [nrde-3p::3xFlag::GFP::nrde-3 ORF + unc-119(+)] IV. Nuclear GFP::NRDE-3 signal. Enhanced RNAi. Lacks RNA editing. Vulval bursting. Low brood size. Triple mutants display bursting and low brood size phenotypes not observed in adr-1;adr-2 or rrf-3 parental strains. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. Mutagen: Outcrossed: x0 Made by: Daniel Reich Received: 03/26/18 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: BB283 Species: Caenorhabditis elegans Genotype: adr-1(uu49) I; adr-2(uu28) III; ergo-1(uu68) V. Description: Enhanced RNAi. Lacks RNA editing. Vulval bursting. Low brood size. Triple mutants display bursting and low brood size phenotypes not observed in adr-1;adr-2 or rrf-3 parental strains. Reference: Reich DP, et al. Genes Dev. 2018 Feb 1;32(3-4):271-282. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Daniel Reich Received: 03/26/18 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: BB3 Species: Caenorhabditis elegans Genotype: adr-2(gv42) III. Description: Defective chemotaxis to volatile odorant. Mutagen: EMS Outcrossed: x8 Made by: Michael Krause Received: 02/05/03 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: BB4 Species: Caenorhabditis elegans Genotype: adr-1(gv6) I; adr-2(gv42) III. Description: Defective chemotaxis to volatile odorant. Low percentage (about 6%) have protruding vulva. Mutagen: Outcrossed: x Made by: Michael Krause Received: 02/05/03 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: BB92 Species: Caenorhabditis elegans Genotype: dcr-1(ok247) III; uuEx18. Description: uuEx18 [dcr-1(wild-type) + dpy-30::mCherry]. Superficially wild-type. Reference: Welker N, et al. (2010) RNA 16:893-903. Mutagen: Outcrossed: x3 Made by: Noah Welker Received: 10/20/10 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: BB93 Species: Caenorhabditis elegans Genotype: dcr-1(ok247) III; uuEx19. Description: uuEx19 [dcr-1(K39A) + dpy-30::mCherry]. Temperature-sensitive, sterile at 25C. Reference: Welker N, et al. (2010) RNA 16:893-903. Mutagen: Outcrossed: x3 Made by: Noah Welker Received: 10/20/10 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: BB94 Species: Caenorhabditis elegans Genotype: dcr-1(ok247) III; uuEx20. Description: uuEx20 [dcr-1(D145N) + dpy-30::mCherry]. Temperature-sensitive, sterile at 25C. Reference: Welker N, et al. (2010) RNA 16:893-903. Mutagen: Outcrossed: x3 Made by: Noah Welker Received: 10/20/10 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: BB95 Species: Caenorhabditis elegans Genotype: dcr-1(ok247) III; uuEx21. Description: uuEx21 [dcr-1(G492R) + dpy-30::mCherry]. Temperature-sensitive, sterile at 25C. Reference: Welker N, et al. (2010) RNA 16:893-903. Mutagen: Outcrossed: x3 Made by: Noah Welker Received: 10/20/10 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: BC10002 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10002. Description: sEx10002[rCesC05D10.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/30/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10004 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10004. Description: sEx10004 [rCes C54H2.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/29/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10008 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10008. Description: sEx10008 [rCesF18E2.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 01/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10010 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx841. Description: sEx841[rCesF43E2.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 10/30/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10011 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx842. Description: sEx842 [rCesC34G6.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 02/19/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10013 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx844. Description: sEx844 [rCesF22E10.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 04/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10016 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx850. Description: sEx850 [rCesT21E8.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 04/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10019 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx853. Description: sEx853 [rCes W09D6.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10021 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx855. Description: sEx855 [rCesC47A10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 05/10/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10023 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx857. Description: sEx857 [rCesDH11.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 10/30/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10024 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx858. Description: sEx858 [rCesZK484.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 10/30/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10027 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx861. Description: sEx861 [rCes Y50E8A.16::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 12/08/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10028 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx862. Description: sEx862[rCesC54D1.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 01/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10030 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx864. Description: sEx864 [rCesC05A9.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 04/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10031 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx865. Description: sEx865 [rCesY43F8C.12::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 02/06/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10034 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx868. Description: sEx868 [rCesT21E8.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 04/24/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10036 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx870. Description: sEx870 [rCes W04C9.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10038 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx872. Description: sEx872[rCesF21G4.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 01/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10042 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10042. Description: sEx10042 [rCes Y53C10A.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10048 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10048. Description: sEx10048 [rCes B0222.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10051 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10051. Description: sEx10051 [rCes F33H2.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/13/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10058 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10058. Description: sEx10058 [rCes C56E6.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10059 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10059. Description: sEx10059 [rCes Y71F9AL.17::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10060 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx884. Description: sEx884 [rCesC12C8.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Reza Pourvali Received: 02/17/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10062 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx890. Description: sEx890 [rCesF11F1.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Reza Pourvali Received: 01/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10064 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx895. Description: sEx895 [rCesC37H5.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Received: 02/17/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10066 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx900. Description: sEx900 [rCesC15H9.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Reza Pourvali Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10068 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx893. Description: sEx893[rCesF54C9.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Reza Pourvali Received: 03/24/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10074 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10074. Description: sEx10074 [rCesK04H4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10075 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10075. Description: sEx10075 [rCesY53C12C.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 05/29/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10077 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10077. Description: sEx10077 [rCesF56C9.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/16/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10089 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10089. Description: sEx10089 [rCesF22E10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 04/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10095 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10095. Description: sEx10095 [rCesF11C3.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 10/25/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10100 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10100. Description: sEx10100 [rCes C26E6.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10101 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10101. Description: sEx10101 [rCesF37A4.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/16/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10102 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10102. Description: sEx10102 [rCes F01G4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 12/08/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10103 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10103. Description: sEx10103 [rCesF52B10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 10/03/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10105 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10105. Description: sEx10105 [rCesZk470.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/11/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10113 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10113. Description: sEx10113 [rCesF46B6.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 07/11/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10116 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10116. Description: sEx10116 [rCesF56B6.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10117 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10117. Description: sEx10117 [rCes Y119C1B.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/10/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10124 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10124. Description: sEx10124 [rCes F56B3.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10128 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10128. Description: sEx10128 [rCes ZC395.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10131 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10131. Description: sEx10131 contains [rCes DH11.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/23/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10133 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10133. Description: sEx10133 [rCes F38A5.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10141 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10141. Description: sEx10141 [rCesY54G2A.17::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 12/21/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10142 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10142. Description: sEx10142 [rCes Y54G2A.17::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/25/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10143 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10143. Description: sEx10143 [rCesF16D3.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/17/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10144 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10144. Description: sEx10144 [rCesC05C8.6:GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/01/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10146 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10146. Description: sEx10146 [rCesF58G6.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 04/18/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10148 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10148. Description: sEx10148 [rCesC30C11.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/10/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10150 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10150. Description: sEx10150 [rCes K06A5.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10152 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10152. Description: sEx10152 [rCes PAR2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10154 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10154. Description: sEx10154 [rCes T05B11.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10157 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10157. Description: sEx10157 [rCesB0303.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/01/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10159 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10159. Description: sEx10159 [rCes B0280.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10160 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10160. Description: sEx10160 [rCesC17H12.14::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 09/19/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10162 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10162. Description: sEx10162[rCesB0511.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10163 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10163. Description: sEx10163 [rCes C28H8.12::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10170 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10170. Description: sEx10170 [rCes R06B10.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10173 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10173. Description: sEx10173 [rCes C17G10.9a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10175 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10175. Description: sEx10175 [rCes Y6D11A.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10178 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10178. Description: sEx10178 [rCesC55B6.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/16/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10180 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10180. Description: sEx10180 [rCes Y94H6A.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10182 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10182. Description: sEx10182 [rCes R13A5.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10183 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10183. Description: sEx10183 [rCes B0252.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10185 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10185. Description: sEx10185 [rCesC09B7.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10190 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10190. Description: sEx10190 [rCesC30F8.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/18/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10191 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10191. Description: sEx10191[rCesY65B4BL.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/10/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10197 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10197. Description: sEx10197 [rCes C01F6.6a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10200 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10200. Description: sEx10200 [rCes F48E8.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/25/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10204 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10204. Description: sEx10204 [rCes C32F10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10206 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10206. Description: sEx10206 [rCesF59C12.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/23/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10210 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10089. Description: sIs10089 [rCes F22E10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10214 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10214. Description: sEx10214 [rCes ZK1248.14::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10220 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10220. Description: sEx10220 [rCes C11D2.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10222 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10222. Description: sEx10222 [rCes C09D4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10225 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10225. Description: sEx10225 [rCes C17E4.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10228 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10228. Description: sEx10228 [rCes F20D12.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10230 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10230. Description: sEx10230 [rCes F53E2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10231 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10231. Description: sEx10231 [rCes F53E2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10232 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10232. Description: sEx10232 [rCes C27C12.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10234 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10234. Description: sEx10234 [rCes C43G2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 09/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10241 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10241. Description: sEx10241 [rCes C49H3.6a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10243 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10243. Description: sEx10243 [rCes W01A11.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10244 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10244. Description: sEx10244 [rCesM01E11.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10249 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10249. Description: sEx10249 [rCesK06H7.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/13/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10253 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10253. Description: sEx10253[rCesB0228.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10255 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10255. Description: sEx10255 [rCesC23G10.4b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/22/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10257 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10257. Description: sEx10257 [rCesZK455.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 09/19/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10261 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10261. Description: sEx10261 [rCes F42G2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10267 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10267. Description: sEx10267 [rCes F45E12.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10273 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10273. Description: sEx10273 [rCes Y37D8A.22::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10276 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10276. Description: sEx10276 [rCes Y45F10A.6a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10278 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10278. Description: sEx10278 [rCesF52C12.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/22/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10280 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10280. Description: sEx10280 [rCes K11C4.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10281 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10281. Description: sEx10281 [rCesF52E1.13::GFP + pCeh361] Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/15/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10285 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10285. Description: sEx10285 [rCesZK795.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 03/11/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10287 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10287. Description: sEx10287 [rCes D2089.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10293 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10293. Description: sEx10293[rCesC47E8.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 02/17/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10295 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10295. Description: sEx10295 [rCes ZC328.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10297 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10297. Description: sEx10297 [rCesM03F8.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10304 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10304. Description: sEx10304 [rCes R07E3.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10306 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10306. Description: sEx10306 [rCes PAR2.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10307 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10307. Description: sEx10307 [rCes Y87G2A.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10312 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10312. Description: sEx10312[rCesC05D11.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10316 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10316. Description: sEx10316 [rCes Y87G2A.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10320 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10320. Description: sEx10320 [rCes B0024.14a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10322 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10322. Description: sEx10322 [rCes K08E4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10326 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10326. Description: sEx10326 [rCes F32D1.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/13/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10331 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10331. Description: sEx10331[rCesB0034.3a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/08/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10336 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10336. Description: sEx10336 [rCes B0035.1b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10338 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10109. Description: sIs10109 [rCesY32H12A.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Bob Johnsen Received: 03/02/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10340 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10340. Description: sEx10340 [rCesR07G3.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 10/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10342 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10342. Description: sEx10342 [rCes T06E8.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10346 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10346. Description: sEx10346 [rCes B0228.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10350 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10350. Description: sEx10350 [rCesB0218.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10352 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10352. Description: sEx10352 [rCesB0035.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 01/27/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10364 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10364. Description: sEx10364 [rCesC44B7.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/30/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10366 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10366. Description: sEx10366 [rCes T02D1.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 02/01/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10368 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10368. Description: sEx10368 [rCes B0280.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10371 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10371. Description: sEx10371 [rCes C53B4.7a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10374 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10374. Description: sEx10374 [rCesB0511.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Allan Mah Received: 01/24/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10375 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10375. Description: sEx10375 [rCes F22B7.::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10376 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10376. Description: sEx10376 [rCesZK688.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Victor Jensen Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10378 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10378. Description: sEx10378 [rCes C05C10.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10379 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10379. Description: sEx10379 [rCesC16A3.10a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/20/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10385 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10385. Description: sEx10385 [rCes ZK632.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10394 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10394. Description: sEx10394 [rCes B0336.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10396 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10396. Description: sEx10396 [rCes C06E7.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10398 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10398. Description: sEx10398 [rCesC06A8.1b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10399 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10399. Description: sEx10399 [rCes C05B10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10400 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10400. Description: sEx10400 [rCesR155.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Allan Mah Received: 10/27/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10407 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10407. Description: sEx10407 [rCes B0412.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/22/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10409 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10409. Description: sEx10409 [rCes B0414.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10416 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10416. Description: sEx10416 contains [rCesB0395.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10417 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10417. Description: sEx10417 [rCes B0361.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10421 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10421. Description: sEx10421[rCesC09G4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10425 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10425. Description: sEx10425 [rCes C09H6.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10427 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10427. Description: sEx10427 [rCesC09F5.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 10/24/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10431 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10431. Description: sEx10431[rCesB0496.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10433 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10433. Description: sEx10433[rCesT21B10.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 12/16/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10434 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10434. Description: sEx10434 [rCes Y46G5A.17::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10435 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10435. Description: sEx10435 [rCesC02F4.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/02/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10442 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10442. Description: sEx10442 [rCes C01G8.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10449 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10449. Description: sEx10449 [rCesB0547.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/13/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10453 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10092. Description: sIs10092[rCesY25C1A.5::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC10460 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10460. Description: sEx10460 [rCesC13C4.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/18/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10464 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10464. Description: sEx10464 [rCes ZK858.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10466 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10466. Description: sEx10466 [rCesC15H9.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/06/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10468 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10468. Description: sEx10468 [rCes C15C7.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10471 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10471. Description: sEx10471 [rCes F40F9.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10475 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10475. Description: sEx10475 [rCesC24A11.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/17/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10477 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10477. Description: sEx10477 [rCesT10E9.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Victor Jensen Received: 02/12/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10482 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10482. Description: sEx10482[rCesY6B3B.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/10/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10484 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10484. Description: sEx10484 [rCesC18D11.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 10/27/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10486 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10486. Description: sEx10486 [rCesZK721.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/02/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10489 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10489. Description: sEx10489 [rCesF36F2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/20/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10495 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10495. Description: sEx10495 [rCesT22B11.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10505 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10505. Description: sEx10505 [rCes ZK792.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10506 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10506. Description: sEx10506 [rCes Y111B2A.18::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10508 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10508. Description: sEx10508 [rCes T04G9.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10509 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10509. Description: sEx10509 [rCes W02C12.3a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10514 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10514. Description: sEx10514 [rCesT05E11.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/17/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10516 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10516. Description: sEx10516 [rCesC47E12.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 12/15/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10518 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10518. Description: sEx10518 [rCes Y17G7B.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10520 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10520. Description: sEx10520 [rCes F08F1.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10525 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10525. Description: sEx10525 [rCesZC404.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 04/04/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10537 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10537. Description: sEx10537 [rCesF10G7.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 02/20/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10538 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10538. Description: sEx10538 [rCes C36B1.12::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10543 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10543. Description: sEx10543[rCesR11A5.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/30/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10545 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10545. Description: sEx10545 [rCesF59A6.::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10550 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10550. Description: sEx10550 [rCes C05D11.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10551 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10551. Description: sEx10551 [rCesF20H11.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/19/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10553 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10553. Description: sEx10553 [rCes C05D11.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10560 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10560. Description: sEx10560 [rCesY50D7A.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10561 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10561. Description: sEx10561 contains [rCes F56H1.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 10/01/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10565 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10565. Description: sEx10565 [rCesY55F3BR.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10568 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10568. Description: sEx10568 [rCes C30F8.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10571 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10571. Description: sEx10571 [rCesT26A5.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/14/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10573 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10573. Description: sEx10573[rCesT26A5.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10574 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10574. Description: sEx10574 [rCesT26A5.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10577 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10577. Description: sEx10577 [rCesF14F3.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 05/11/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10579 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10136. Description: sIs10136 [rCes C41D11.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10588 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10126. Description: sIs10126 [rCes Y47G6A.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10589 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10109. Description: sIs10109 [rCesY32H12A.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 03/02/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10594 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10594. Description: sEx10594 [rCesF35G12.3B::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10600 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10600. Description: sEx10600 [rCes C05D11.11a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10604 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10325. Description: sIs10325 [rCesC36A4.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Victor Jensen Received: 05/22/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10607 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10607. Description: sEx10607 [rCesF12F6.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 02/07/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10611 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10611. Description: sEx10611 [rCes F20D12.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10612 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10612. Description: sEx10612 [rCes Y75B8A.12::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10614 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10614. Description: sEx10614 [rCes T25F10.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10615 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10615. Description: sEx10615 contains [rCesT01C8.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10618 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10618. Description: sEx10618 contains [rCesT05C12.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 12/01/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10621 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10621. Description: sEx10621[rCesF54G8.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/28/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10623 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10623. Description: sEx10623 [rCesF54A5.3a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10629 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10629. Description: sEx10629 [rCesY39A1A.15b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10634 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10634. Description: sEx10634 [rCes Y39G10AR.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10640 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10640. Description: sEx10640 [rCesY47H9C.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 05/28/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10642 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10642. Description: sEx10642[rCesC29E6.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/06/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10650 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10247. Description: sIs10247 [rCes ZC13.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10652 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10253. Description: sIs10253 [rCesB0228.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: J Lin & L Halim Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10653 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10259. Description: sIs10259 [rCesY32H12A.8::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC10655 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10655. Description: sEx10655 [rCes B0336.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 01/25/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10656 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10656. Description: sEx10656 [rCes K08D12.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 01/27/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10660 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10660. Description: sEx10660 [rCesF15C11.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/01/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10661 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10661. Description: sEx10661[rCesY48C3A.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10665 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10665. Description: sEx10665 [rCes Y48B6A.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10671 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10137. Description: sIs10137 [rCesM03F4.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 12/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10672 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10263. Description: sIs10263[rCesF35H8.6::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC10674 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10107. Description: sIs10107 [rCes F32A6.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10675 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10135. Description: sIs10135 [rCesZK177.8a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakkan Received: 10/20/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10680 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10083. Description: sIs10083[rCesF55A12.8::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC10688 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10688. Description: sEx10688 [rCes Y116A8A.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10689 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10689. Description: sEx10689 [rCesY25C1A.13::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10691 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10691. Description: sEx10691[rCesY54E5A.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/15/25 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10697 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10697. Description: sEx10697 [rCesZC101.2e::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Emily Ha Received: 03/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10699 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10699. Description: sEx10699 [rCes ZK154.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC107 Species: Caenorhabditis elegans Genotype: bli-4(e937) dpy-14(e188) I. Description: Blistered. Dpy (ts). Mutagen: Outcrossed: x Made by: Ann Rose Received: 10/06/99 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10700 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10700. Description: sEx10700 [rCesZK632.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Allan Mah Received: 03/11/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10709 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10112. Description: sIs10112 [rCes Y69A2AR.18::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 10/04/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10711 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10218. Description: sIs10218 [rCes C13F10.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10713 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10193. Description: sIs10193[rCesZK822.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 06/18/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10715 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10236. Description: sIs10236 [rCes Y32H12A.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10716 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10236. Description: sIs10236 [rCesY32H12A.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 09/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10717 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10503. Description: sIs10503 [rCesY71H2B.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Robert Johnsen Received: 06/20/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10718 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10246. Description: sIs10246 [rCesF10E9.6b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 01/11/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10719 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10576. Description: sIs10576 contains [rCes F11D5.3a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Robert Johnson Received: 10/01/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10720 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10523. Description: sIs10523[rCesC18H9.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Johnsen Received: 02/08/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10721 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10524. Description: sIs10524 [rCes C17H12.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10723 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10723. Description: sEx10723 [rCes C24A1.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10732 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10623. Description: sIs10623[rCesF54A5.3a::GFP + pCeh361]. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-rays Outcrossed: x0 Made by: Victor Jensen Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10733 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10623. Description: sEx10623[rCesF54A5.3a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC10734 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10734. Description: sEx10734 [rCes T16G1.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10744 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10744. Description: sEx10744 [rCesC48A7.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 04/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10749 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10749. Description: sEx10749 [rCes F08B12.3b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/07/13 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10751 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10751. Description: sEx10751[rCesC44B11.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Allan Mah Received: 06/19/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10753 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10753. Description: sEx10753 [rCes Y57G11C.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10754 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10754. Description: sEx10754 [rCes C09G12.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 01/27/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10760 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10760. Description: sEx10760 [rCesY54F10AM.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10766 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10766. Description: sEx10766 [rCes F20D12.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10770 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10239. Description: sIs10239 [rCes C28H8.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10773 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10773. Description: sEx10773 [rCes B0250.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10784 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10784. Description: sEx10784 contains [rCes C07H4.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 08/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10785 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10785. Description: sEx10785 contains [rCes Y108G3AL.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: L. Fang/Z. Zhao Received: 10/21/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10787 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10787. Description: sEx10787 [rCes Y18D10A.13::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10792 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10792. Description: sEx10792[rCesY55F3AL.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: L Fang & Z Zhao Received: 04/18/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10795 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10795. Description: sEx10795 [rCesY39B6A.20::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: L Fang & Z Zhao Received: 02/17/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10796 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10796. Description: sEx10796 [rCes ZC395.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/08/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10804 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10804. Description: sEx10804 [rCes Y67H2A.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10809 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10564. Description: sIs10564 contains [rCes T26C12.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 10/01/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10812 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10602. Description: sIs10602 contains [rCes K07A9.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 08/29/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10814 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10814. Description: sIs10814 [rCesT06H11.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-ray Outcrossed: x Made by: Robert Hollebakken Received: 10/27/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10818 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10693. Description: sIs10693[rCesY54E2A.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 11/20/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10819 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10697. Description: sIs10697 [rCesZC101.2e::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). sIs10697 [rCesZC101.2e::GFP + pCeh361] Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 03/02/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10825 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10825. Description: sEx10825 [rCes D2030.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10829 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10829. Description: sEx10829 [rCesF16B3.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10830 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10830. Description: sEx10830 [rCes C26C6.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/13/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10836 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10836. Description: sEx10836 [rCesT28F2.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 11/14/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10837 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10837. Description: sEx10837 [rCesB0334.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 09/12/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10838 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10838. Description: sEx10838 [rCes C05C8.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10848 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10703. Description: sIs10703[rCesR09F10.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 07/24/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10849 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10707. Description: sIs10707 [rCes F28H7.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10853 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10623. Description: sIs10623 [rCesF54A5.3A::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Victor Jensen Received: 08/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10856 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10123. Description: sIs10123[rCesT10H9.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Victor Jensen Received: 02/02/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10860 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10860. Description: sEx10860 [rCes M01F1.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10862 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10862. Description: sEx10862 [rCes T04A8.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 12/01/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10873 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10873. Description: sEx10873 [rCes ZC506.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10874 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10874. Description: sEx10874 [rCesC01G8.5a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10891 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10891. Description: sEx10891 [rCes C16C10.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10902 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10902. Description: sEx10902 [rCes C18E9.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10909 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10909. Description: sEx10909 [rCes W01A11.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10910 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10910. Description: sEx10910 [rCesT20F10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 08/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10912 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10912. Description: sEx10912[rCesT07C4.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 01/03/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10917 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10917. Description: sEx10917 [rCes Y43B11AR.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10929 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10929. Description: sEx10929 [rCes C27H5.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 01/14/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10933 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10933. Description: sEx10933 [rCesF34H10.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/16/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10950 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10950. Description: sEx10950 [rCesF38H4.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Emily Ha Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10952 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10952. Description: sEx10952[rCesR09B5.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10981 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10981. Description: sEx10981 [rCes Y71G12B.16::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC10999 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx10999. Description: sEx10999 [rCes D1054.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC110 Species: Caenorhabditis elegans Genotype: dpy-14(e188) unc-13(e51) let-85(s142)/unc-15(e73) I. Description: Heterozygotes are WT and segregate WT, Unc and DpyUncLets. Lethal at L1. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Ann Rose Received: 01/26/93 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC11000 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11000. Description: sEx11000 [rCes ZC412.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11002 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11002. Description: sEx11002 [rCes C56C10.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/25/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11003 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11003. Description: sEx11003[rCesD2030.9a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11009 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11009. Description: sEx11009 [rCesZK970.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: AllanMah Received: 11/05/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11010 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11010. Description: sEx11010 [rCesZK792.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 04/18/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11018 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11018. Description: sEx11018 [rCes C38C10.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11019 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11019. Description: sEx11019 [rCesC44B12.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11021 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11021. Description: sEx11021 [rCes F28F8.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11024 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11024. Description: sEx11024 [rCes F28D1.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11025 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11025. Description: sEx11025 [rCes F27D4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11026 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11026. Description: sEx11026 [rCesF27C1.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11027 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11027. Description: sEx11027 [rCesF26D10.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/15/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11040 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11040. Description: sEx11040 [rCes F22D6.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/19/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11042 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11042. Description: sEx11042 [rCes F13E6.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11043 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11043. Description: sEx11043 [rCesF14D12.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11044 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11044. Description: sEx11044 [rCesF10B5.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11048 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11048. Description: sEx11048 [rCes F11A10.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11054 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11054. Description: sEx11054 [rCesF31C3.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/15/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11057 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11057. Description: sEx11057 [rCes F31D4.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11059 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11059. Description: sEx11059 [rCesF38A6.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/25/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11061 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11061. Description: sEx11061 [rCes F39B2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11062 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11062. Description: sEx11062[rCesF37C12.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/15/25 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11063 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11063. Description: sEx11063 [rCes F38A5.13::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11065 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11065. Description: sEx11065 [rCesF42D1.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/02/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11068 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11068. Description: sEx11068 [rCesF46A9.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 04/07/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11070 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11070. Description: sEx11070 [rCes C44E4.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11072 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11072. Description: sEx11072[rCesC46F11.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/22/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11078 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11078. Description: sEx11078 [rCes C02B10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11085 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11085. Description: sEx11085 [rCesF46E10.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/21/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11090 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11090. Description: sEx11090 [rCes ZK512.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11098 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11098. Description: sEx11098 [rCes Y62E10A.13c::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11100 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11100. Description: sEx11100 [rCes C18C4.10b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11102 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11102. Description: sEx11102 [rCes R02F2.1b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11105 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11105. Description: sEx11105 [rCes C37E2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11106 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11106. Description: sEx11106 [rCes Y50D7A.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11108 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11108. Description: sEx11108 [rCes C28H8.11b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11109 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11109. Description: sEx11109 [rCes B0416.5a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11110 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11110. Description: sEx11110 [rCesT04C12.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/03/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11111 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11111. Description: sEx11111[rCesC32F10.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 10/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11116 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11116. Description: sEx11116 [rCesT10H9.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/24/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11120 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11120. Description: sEx11120 [rCesF53A3.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/08/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11124 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11124. Description: sEx11124 [rCesF47D12.4b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 05/22/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11128 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11128. Description: sEx11128 [rCesK10B3.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 02/22/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11129 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11129. Description: sEx11129 [rCesF41E7.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Allan Mah Received: 10/27/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11137 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11137. Description: sEx11137 [rCesF43H9.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11139 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11139. Description: sEx11139 [rCes R11E3.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11142 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11142. Description: sEx11142[rCesY48D7A.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/07/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11150 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11150. Description: sEx11150 [rCesF38B7.1B::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11161 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11161. Description: sEx11161 [rCesH21P03.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/18/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11164 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11164. Description: sEx11164 [rCes K02F2.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11168 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11168. Description: sEx11168 [rCesZK1127.9b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 04/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11172 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11172. Description: sEx11172 [rCes C33H5.18b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11178 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11178. Description: sEx11178 [rCes R166.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11180 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11180. Description: sEx11180 [rCes C10G8.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 01/27/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11182 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11182. Description: sEx11182[rCesZK381.5a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11183 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11183. Description: sEx11183[rCesZK381.5a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/14/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11186 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11186. Description: sEx11186 [rCesC54D1.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 04/18/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11190 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11190. Description: sEx11190 [rCes C44C1.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11193 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11193. Description: sEx11193 [rCes C50D2.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11195 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11195. Description: sEx11195 [rCesC39F7.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/20/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11198 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11198. Description: sEx11198 [rCesT23B12.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC112 Species: Caenorhabditis elegans Genotype: dpy-14(e188) unc-13(e51) let-82(s85)/unc-15(e73) I. Description: Heterozygotes are WT and segregate more WT, paralyzed Unc, and DpyUncLet (DpyUnc Larvae are abnormal). Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Ann Rose Received: 09/01/82 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC11201 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10158. Description: sIs10158 [rCesF54D8.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-rays Outcrossed: x0 Made by: Robert Hollebakken Received: 10/24/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11202 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10158. Description: sIs10158 [rCes F54D8.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11205 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10309. Description: sIs10309 [rCes F27D9.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11206 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10309. Description: sIs10309 [rCes F27D9.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11207 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10648. Description: sIs10648 [rCes Y45F3A.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11215 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10171. Description: sIs10171 [rCes C30F12.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11217 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10139. Description: sIs10139 [rCes F10E7.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11221 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11221. Description: sEx11221[rCesF28D1.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/08/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11234 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11234. Description: sEx11234 [rCes C02E11.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11238 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11238. Description: sEx11238 [rCesH39E23.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 01/04/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11239 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11239. Description: sEx11239 [rCesR11H6.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 02/25/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11241 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11241. Description: sEx11241 [rCes T22D1.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11245 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11245. Description: sEx11245 [rCes Y55D9A.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11253 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11253. Description: sEx11253 [rCes T03F6.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11255 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11255. Description: sEx11255 [rCesF19B6.1b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/03/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11264 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11264. Description: sIs11264 [rCesK07A3.1::GFP + pCeh361]. Inserted array seems to be unstable or prone to silencing. Pick non-Dpy GFP+ animals to maintain array. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). [NOTE: Though this strain is reported as carrying an integrated array, this strain segregates both GFP+ non-Dpy and GFP- Dpy.] Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/19/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11267 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11267. Description: sEx11267 [rCesK10G6.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/24/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11269 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11269. Description: sEx11269 [rCesR05F9.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/20/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11271 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11271. Description: sEx11271 [rCes R01H10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 07/13/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11274 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11274. Description: sEx11274 [rCesM163.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11281 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11281. Description: sEx11281 [rCes R07H5.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11286 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11286. Description: sEx11286 [rCesK07H8.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/04/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11288 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11288. Description: sEx11288 [rCes W02C12.3b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 07/24/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11290 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11290. Description: sEx11290 [rCesC04F6.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 12/13/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11293 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11293. Description: sEx11293 [rCes Y71H10B.1b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11301 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11301. Description: sEx11301[rCesR13A5.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11304 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11304. Description: sEx11304 [rCesR09E12.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/16/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11306 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11306. Description: sEx11306 [rCesT03E6.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/20/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11307 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11307. Description: sEx11307 [rCes T05A7.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11309 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11309. Description: sEx11309 [rCes T22C1.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11314 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11314. Description: sEx11314 [rCesT20G5.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/15/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11317 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11317. Description: sEx11317 [rCes T22H6.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11318 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11318. Description: sEx11318 [rCes T21B10.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11319 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11319. Description: sEx11319 [rCes T21D12.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11321 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11321. Description: sEx11321 [rCes T23G5.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11322 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11322. Description: sEx11322 [rCes T25G12.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11324 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11324. Description: sEx11324 [rCesT23G5.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/06/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11334 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11334. Description: sEx11334 [rCes W09C5.6a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11336 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11336. Description: sEx11336 [rCesT10B11.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/16/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11338 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11338. Description: sEx11338 [rCes Y54E10A.9a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/05/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11353 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10295. Description: sIs10295 [rCesZC328.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 08/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11356 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10402. Description: sIs10402 contains [rCes C08B6.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Robert Hollebakken Received: 10/01/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11358 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10334. Description: sIs10334 [rCesC13B9.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 02/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11360 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10905. Description: sIs10905 [rCesT27E9.4a::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC11361 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10905. Description: sIs10905 [rCes T27E9.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11364 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10402. Description: sIs10402[rCesC08B6.8::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC11365 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10402. Description: sIs10402 [rCes C08B6.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11367 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10403. Description: sIs10403[rCesF32F2.1::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC11369 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11369. Description: sEx11369 [rCesT03G6.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11374 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11374. Description: sEx11374 [rCes F53A9.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 04/19/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11393 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11393. Description: sEx11393 [rCes F26H9.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11395 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11395. Description: sEx11395 [rCesT19E7.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 01/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11397 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11397. Description: sEx11397 [rCes C01B7.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11408 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11408. Description: sEx11408 [rCes B0414.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11409 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11409. Description: sEx11409 [rCesF10B5.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11410 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11410. Description: sEx11410 [rCes M04B2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 04/19/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11412 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11412. Description: sEx11412 [rCesY51H4A.17::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/16/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11413 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11413. Description: sEx11413 [rCes Y76B12C.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11416 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11416. Description: sEx11416 [rCesR07B7.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Emily Ha Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11417 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11417. Description: sEx11417 [rCesK11E8.1d::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Emily Ha Received: 01/18/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11418 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11418. Description: sEx11418 [rCes F33C8.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11419 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11419. Description: sEx11419 [rCesK03F8.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11420 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11420. Description: sEx11420 [rCes C09C7.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11424 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11424. Description: sEx11424 [rCes E02H4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11425 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11425. Description: sEx11425 contains [rCes Y39E4B.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 08/26/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11430 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11430. Description: sEx11430 [rCesF22E10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/03/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11436 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11436. Description: sEx11436 [rCesC33A11.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/10/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11438 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11438. Description: sEx11438 [rCesC05E4.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11439 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11439. Description: sEx11439 [rCesC36E8.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/02/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11445 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10122. Description: sIs10122 [rCes K02A4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11450 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11450. Description: sEx11450 [rCesK08C7.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11452 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11452. Description: sEx11452 [rCes R09A1.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11466 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11466. Description: sEx11466 [rCesF13B10.1b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 02/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11468 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11468. Description: sEx11468 [rCes F48C11.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11472 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11472. Description: sEx11472 [rCes C55H1.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Allan Mah Received: 12/28/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11473 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11473. Description: sEx11473[rCesT23H2.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 04/11/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11475 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11475. Description: sEx11475 [rCesF56D12.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Emily Ha Received: 04/18/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11477 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11477. Description: sEx11477 [rCes F55D10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11480 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11480. Description: sEx11480 [rCes F55C7.7b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11487 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11487. Description: sEx11487 [rCesZC53.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 09/25/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11493 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11493. Description: sEx11493[rCesZK1127.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 02/20/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11494 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11494. Description: sEx11494 [rCesF26E4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/08/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11495 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11495. Description: sEx11495 [rCes F33H2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 10/01/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11497 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11497. Description: sEx11497 [rCes F56H9.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/28/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC115 Species: Caenorhabditis elegans Genotype: dpy-14(e188) unc-13(e51) let-81(s88)/unc-15(e73) I. Description: Heterozygotes are WT and segregate more WT, paralyzed Unc and DpyUncLethals. The DpyUncLets are abnormal larvae and die in early larval development. Pick WT to maintain. Mutagen: EMS Outcrossed: x Made by: Ann Rose Received: 09/01/82 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC11504 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11504. Description: sEx11504 [rCesY8G1A.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 07/11/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11507 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11507. Description: sEx11507 [rCes T21H3.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11510 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10411. Description: sIs10411[rCes B0457.1::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 06/20/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11512 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10392. Description: sIs10392[rCesB0334.4::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC11513 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10779. Description: sIs10779 [rCes C04G6.3::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Bombardment Outcrossed: x0 Made by: Robert Hollebakken Received: 01/23/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11515 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10781. Description: sIs10781[rCesC10H11.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 04/16/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11518 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10379. Description: sIs10379 [rCesC16A3.10a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 02/20/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11520 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10358. Description: sIs10358 [rCesY102A11A.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 02/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11521 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10354. Description: sIs10354 [rCesY39D8C.1::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC11522 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10354. Description: sIs10354 [rCesY39D8C.1::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC11525 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10330. Description: sIs10330 [rCesB0034.3a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 06/08/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11529 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11529. Description: sEx11529 [rCes F48G7.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11539 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11539. Description: sEx11539 [rCesF14D12.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/28/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11545 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11545. Description: sEx11545 [rCesK02F2.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/31/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11552 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11552. Description: sEx11552[rCesF54G8.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/11/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11562 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11562. Description: sEx11562 [rCes Y32G9A.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11564 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11564. Description: sEx11564 [rCesC02A12.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/06/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11566 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11566. Description: sEx11566 [rCesY19D10A.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/25/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11571 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11571. Description: sEx11571 contains [rCes F39G3.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Allan Mah Received: 12/01/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11580 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11580. Description: sEx11580 [rCes JC8.10b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11584 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11584. Description: sEx11584 [rCesK08E3.5b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Emily Ha Received: 03/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11587 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10415. Description: sIs10415 [rCes B0395.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 11/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11588 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11330. Description: sIs11330 [rCesW02D3.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 02/06/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11598 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11598. Description: sEx11598 [rCesF58G4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 09/24/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11600 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11600. Description: sEx11600 [rCesE03G2.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11601 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11601. Description: sEx11601 [rCes M01A10.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11603 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11603. Description: sEx11603[rCesT12A2.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/06/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11610 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11610. Description: sEx11610 [rCesZK287.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11611 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11611. Description: sEx11611 [rCes F40F9.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11613 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11613. Description: sEx11613[rCesM02B7.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11617 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11617. Description: sEx11617 [rCes W01A11.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/15/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11620 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11620. Description: sEx11620 [rCes R13H4.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Emily Ha Received: 02/06/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11626 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11626. Description: sEx11626 [rCes T13H5.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/25/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11630 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11630. Description: sEx11630 [rCesC07H6.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/31/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11631 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11631. Description: sEx11631[rCesY57E12AL.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/06/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11648 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11648. Description: sEx11648 [rCesC06C6.5a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/31/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11649 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11649. Description: sEx11649 [rCesF40F8.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11654 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11654. Description: sEx11654 [rCesW06H8.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11659 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11659. Description: sEx11659 [rCes M106.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11660 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11660. Description: sEx11660 [rCes F10D7.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11661 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11661. Description: sEx11661 [rCes Y73F8A.24::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11664 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11664. Description: sEx11664 [rCes C08F11.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11667 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11667. Description: sEx11667 [rCesW05E10.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 02/20/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11669 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11669. Description: sEx11669 [rCes C25E10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11679 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11679. Description: sEx11679 [rCes C50F2.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11688 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11688. Description: sEx11688 [rCes F39H12.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11694 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11694. Description: sEx11694 contains [rCes ZK856.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Zhongying Zhao Received: 10/21/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11696 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11696. Description: sEx11696 contains [rCes C04F6.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Zhongying Zhao Received: 11/18/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11699 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11699. Description: sEx11699 [rCesY62F5A.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/20/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11701 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11701. Description: sEx11701 [rCes C01F1.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11706 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11706. Description: sEx11706 [rCesF36A2.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11709 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11709. Description: sEx11709 [rCesC27H5.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Emily Ha Received: 04/22/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11716 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11716. Description: sEx11716 [rCesZK637.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/18/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11729 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11729. Description: sEx11729 [rCes Y49E10.20::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11730 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11730. Description: sEx11730 [rCesF48E8.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 01/20/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11735 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11735. Description: sEx11735 [rCesY82E9BR.14::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11736 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11736. Description: sEx11736 [rCes H28G03.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11742 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11742. Description: sEx11742[rCesT04C9.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11751 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11751. Description: sEx11751[rCesF53F10.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 04/24/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11760 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11760. Description: sEx11760 [rCesF55A12.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Allan Mah Received: 06/20/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11763 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11763. Description: sEx11763 [rCesK04F10.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 10/24/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11770 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11770. Description: sEx11770 [rCes F49E12.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11772 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11772. Description: sEx11772 [rCes C49C8.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11774 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11774. Description: sEx11774 [rCesB0035.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/31/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11776 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11776. Description: sEx11776 [rCes C10H11.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11778 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11778. Description: sEx11778 [rCes F26E4.12::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11779 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11779. Description: sEx11779 [rCes K07E3.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11780 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11780. Description: sEx11780 [rCesB0496.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/16/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11787 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11787. Description: sEx11787 [rCes C34G6.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11792 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11792. Description: sEx11792[rCesD2013.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Allan Mah Received: 12/22/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11793 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11793. Description: sEx11793[rCesD2085.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11796 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11796. Description: sEx11796 [rCes F22F4.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11799 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11799. Description: sEx11799 [rCesF56E3.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/24/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11803 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11803. Description: sEx11803 [rCesEEED8.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Emily Ha Received: 07/10/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11808 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11808. Description: sEx11808 [rCes F38E9.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11815 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11815. Description: sEx11815 [rCes Y52B11A.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11817 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11817. Description: sEx11817 [rCes Y56A3A.19::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11822 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11822. Description: sEx11822 [rCes T12A2.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11824 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11824. Description: sEx11824 [rCesZK1098.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11825 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11825. Description: sEx11825 [rCes ZK1098.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11831 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11831. Description: sEx11831[rCesK11D2.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11840 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10741. Description: sIs10741[rCesC50F2.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Simon Wong Received: 10/20/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11841 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11122. Description: sIs11122 [rCes T22B2.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11844 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11844. Description: sEx11844 [rCes F18F11.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11847 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11847. Description: sEx11847 [rCesW02D7.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/18/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11848 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11848. Description: sEx11848 [rCes Y24F12A.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11849 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11849. Description: sEx11849 [rCes C30F12.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11851 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11851. Description: sEx11851 [rCes C32E8.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11853 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11853. Description: sEx11853 [rCes ZC64.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11855 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11855. Description: sEx11855 [rCes C17G10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11856 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11856. Description: sEx11856 [rCesCD4.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 10/24/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11857 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11857. Description: sEx11857 [rCes C15C7.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 01/10/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11858 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10428. Description: sIs10428 [rCes C10C6.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-rays Outcrossed: x0 Made by: John Lin/Leny Halim Received: 01/10/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11859 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11859. Description: sEx11859 [rCesC53D6.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11862 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11862. Description: sEx11862[rCesF32B6.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 10/31/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11864 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11864. Description: sEx11864 [rCes T13H10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11868 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11868. Description: sEx11868 [rCes T27A8.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11871 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11871. Description: sEx11871 [rCes T01G1.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11875 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11875. Description: sEx11875 [rCesZC168.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11878 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11878. Description: sEx11878 [rCesC06G8.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/02/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11883 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11883. Description: sEx11883 [rCes ZK792.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11886 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11886. Description: sEx11886 [rCesY47D3A.25::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 06/06/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11887 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11887. Description: sEx11887 [rCesY75B8A.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 04/18/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11893 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11893. Description: sEx11893 [rCes F20D6.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11894 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11894. Description: sEx11894 [rCes C15C8.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11897 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11897. Description: sEx11897 [rCesY47D3A.6a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11898 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11898. Description: sEx11898 [rCesF44D12.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 03/02/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC119 Species: Caenorhabditis elegans Genotype: blmp-1(s71) I. Description: Dpy. Mutagen: EMS Outcrossed: x Made by: Rose A Received: 09/01/82 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11902 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11902. Description: sEx11902[rCesM18.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11904 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11904. Description: sEx11904 [rCesB0001.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/21/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11906 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11906. Description: sEx11906 [rCes F35G2.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11909 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11909. Description: sEx11909 [rCesK09E10.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11911 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11911. Description: sEx11911 [rCes T04A11.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11922 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11922. Description: sEx11922 [rCes W02A2.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11923 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11923. Description: sEx11923 [rCesY62E10A.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11926 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10429. Description: sIs10429 [rCes C12D8.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Simon Wong Received: 09/01/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11928 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10491. Description: sIs10491[rCesR12B2.5a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Simon Wong Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11936 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11936. Description: sEx11936 [rCes Y105C5B.21::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/02/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11937 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11937. Description: sEx11937 [rCesF54C8.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11938 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11938. Description: sEx11938 [rCesF54D5.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 04/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11941 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11941. Description: sEx11941[rCesF23B12.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 04/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11945 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11945. Description: sEx11945 [rCesF43E2.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Emily Ha Received: 02/22/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11946 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11946. Description: sEx11946 [rCes F30H5.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11948 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11948. Description: sEx11948 [rCesF08F3.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/18/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11949 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11949. Description: sEx11949 [rCes C01H6.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11958 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11958. Description: sEx11958 [rCes AH6.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11960 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11960. Description: sEx11960 [rCesC35D10.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 04/06/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11964 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11964. Description: sEx11964 [rCesY67D2.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Emily Ha Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11970 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11970. Description: sEx11970 [rCesC37C3.6a::GFP + pCeh361] Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 05/31/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11973 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11973. Description: sEx11973 [rCes C50E10.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11976 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11976. Description: sEx11976 [rCes B0304.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11985 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11985. Description: sEx11985 [rCes F37C12.17::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11990 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11990. Description: sEx11990 [rCesY106G6E.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Zhongying Zhao Received: 01/24/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11995 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11995. Description: sEx11995 [rCesF59A2.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Emily Ha Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC11999 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11999. Description: sEx11999 [rCes F57G9.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12002 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12002. Description: sEx12002 [rCes Y39G8B.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12003 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12003. Description: sEx12003 [rCes F58A6.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12005 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12005. Description: sEx12005 [rCes T21H8.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12008 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12008. Description: sEx12008 [rCes Y57A10B.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12009 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12009. Description: sEx12009 [rCes ZK265.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1201 Species: Caenorhabditis elegans Genotype: sDf19/nT1 IV; +/nT1 V. Description: sDf19 has a dominant twitcher phenotype, and is homozygous lethal-usually arresting as embryos, but occasionally will hatch. Heterozygotes are twitchers and segregate twitchers, Vulvaless and dead eggs (or lethal early larvae). This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Outcrossed: x Made by: T. Rogalski Received: 10/01/84 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12012 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12012. Description: sEx12012 [rCes C47A10.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12016 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12016. Description: sEx12016 [rCes T20D4.18::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12017 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12017. Description: sEx12017 [rCesZK637.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/22/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12019 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12019. Description: sEx12019 [rCes F56G4.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12021 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12021. Description: sEx12021[rCesT12A2.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 07/18/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12028 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12028. Description: sEx12028 [rCesF57C12.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 02/18/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12036 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12036. Description: sEx12036 [rCes F22E10.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 12/08/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12037 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12037. Description: sEx12037 [rCes Y34D9A.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12039 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12039. Description: sEx12039 contains [rCes ZK121.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Allan Mah Received: 08/26/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12045 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12045. Description: sEx12045 [rCes T20D4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12047 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12047. Description: sEx12047 [rCes C33A12.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12048 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12048. Description: sEx12048 [rCes H10D18.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12051 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12051. Description: sEx12051 [rCes Y47D3A.6b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12068 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12068. Description: sEx12068 [rCesK07B1.5b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12073 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12073. Description: sEx12073 [rCes B0041.7a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12075 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12075. Description: sEx12075 [rCesF12F6.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Allan Mah Received: 01/24/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12078 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12078. Description: sEx12078 [rCes T08G11.1b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12079 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12079. Description: sEx12079 [rCes B0285.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12082 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12082. Description: sEx12082 [rCes F25F2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Emily Ha Received: 08/25/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12095 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12095. Description: sEx12095 [rCes F28F5.3a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12098 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12098. Description: sEx12098 [rCes C05B5.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12101 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12101. Description: sEx12101[rCesF42A9.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 12/17/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12103 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12103. Description: sEx12103 contains [rCes F42G10.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Zhongying Zhao Received: 10/12/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12107 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12107. Description: sEx12107 [rCesC47C12.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12110 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12110. Description: sEx12110 [rCesT07H3.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12111 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12111. Description: sEx12111 [rCes C03G5.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12113 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12113. Description: sEx12113[rCesW07A12.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12115 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12115. Description: sEx12115 [rCesC24G7.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Zhongying Zhao Received: 06/19/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12117 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12117. Description: sEx12117 [rCesF46F11.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12120 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12120. Description: sEx12120 [rCesF08G2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/31/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12128 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12128. Description: sEx12128 [rCesY59C2A.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 01/20/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12133 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12133. Description: sEx12133 [rCes C50E10.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12138 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12138. Description: sEx12138 [rCes F09E10.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12139 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12139. Description: sEx12139 [rCesY48A6A.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 11/27/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12142 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12142. Description: sEx12142[rCesR03E9.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Zhongying Zhao Received: 10/28/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12145 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12145. Description: sEx12145 [rCes Y45G12C.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 02/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12146 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12146. Description: sEx12146 [rCesB0379.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 08/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12152 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12152. Description: sEx12152[rCesF20B6.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 09/05/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12154 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12154. Description: sEx12154 [rCes F52F12.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 01/10/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12158 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12158. Description: sEx12158 [rCes F09F7.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12159 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12159. Description: sEx12159 [rCes C27F2.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1216 Species: Caenorhabditis elegans Genotype: sDf21 dpy-4/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Gamma Rays Outcrossed: x Made by: Wild G Received: 01/14/92 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12162 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12162. Description: sEx12162[rCesH13N06.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 10/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1217 Species: Caenorhabditis elegans Genotype: sDf22/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Gamma Rays Outcrossed: x Made by: G. Wild Received: 12/15/87 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12173 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12173. Description: sEx12173[rCesT21H8.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 07/24/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12175 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12175. Description: sEx12175 [rCesC36C5.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 07/24/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12177 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12177. Description: sEx12177 [rCes C33G8.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12181 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12181. Description: sEx12181[rCesM01H9.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/03/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12182 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12182. Description: sEx12182 [rCes T20D4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12187 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12187. Description: sEx12187 [rCes Y53C10A.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12188 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12188. Description: sEx12188 [rCesC02B8.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12190 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12190. Description: sEx12190 [rCesF11C3.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12191 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12191. Description: sEx12191[rCesT17E9.1b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Emily Ha Received: 05/26/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12194 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12194. Description: sEx12194 [rCes Y39C12A.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12195 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12195. Description: sEx12195 [rCes Y39C12A.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12198 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12198. Description: sEx12198 [rCes T07H8.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12200 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12200. Description: sEx12200 [rCes F57G9.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12201 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12201. Description: sEx12201 [rCes R07B7.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12205 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12205. Description: sEx12205 [rCesC26F1.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 08/16/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12211 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12211. Description: sEx12211 [rCes C50E10.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/25/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12212 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12212. Description: sEx12212 [rCes AH6.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12220 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12220. Description: sEx12220 [rCes C34H4.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12224 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12224. Description: sEx12224 [rCesC08A9.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 04/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12225 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12225. Description: sEx12225 [rCesY47D3A.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12229 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12229. Description: sEx12229 [rCesY37B11A.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Emily Ha Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12230 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12230. Description: sEx12230 [rCes F13G3.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12231 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12231. Description: sEx12231[rCesE04F6.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Emily Ha Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12233 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12233. Description: sEx12233[rCesC13G5.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Emily Ha Received: 08/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12234 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12234. Description: sEx12234 [rCesC13G5.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Emily Ha Received: 05/21/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12236 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12236. Description: sEx12236 [rCesY76A2B.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12239 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12239. Description: sEx12239 [rCesD1081.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 04/10/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12246 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12246. Description: sEx12246 [rCes F54F11.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12251 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12251. Description: sEx12251 [rCes R144.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12268 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12268. Description: sEx12268 [rCesC35D10.16::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 05/03/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1227 Species: Caenorhabditis elegans Genotype: sDf23/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Gamma Rays Outcrossed: x Made by: Dave Baillie Received: 01/05/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12270 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12270. Description: sEx12270 [rCesF46F3.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12275 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12275. Description: sEx12275 [rCesM142.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12276 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10439. Description: sIs10439 [rCesC03C10.1::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Simon Wong Received: 10/01/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12278 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10460. Description: sIs10460 [rCes C13C4.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Simon Wong Received: 10/03/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12279 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10437. Description: sIs10437 [rCesC02E11.1a::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12285 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12285. Description: sEx12285 [rCes C27D6.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12286 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12286. Description: sEx12286 [rCes C27D6.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12299 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12299. Description: sEx12299 [rCes C50C3.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/29/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC123 Species: Caenorhabditis elegans Genotype: dpy-14(e188) unc-13(e51) let-84(s91)/unc-15(e73) I. Description: Heterozygotes are WT and segregate more WT, paralyzed Unc and DpyUncLet. The DpyUncs are abnormal larvae that die in late larval development. Pick WT to maintain. Mutagen: EMS Outcrossed: x Made by: Ann Rose Received: 09/01/82 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC1230 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf27 unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Gamma Rays Outcrossed: x Made by: R. Rosenbluth Received: 07/01/85 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12301 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10475. Description: sIs10475 [rCesC24A11.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Simon Wong Received: 05/31/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12302 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10480. Description: sIs10480 [rCes T28F3.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Simon Wong Received: 09/19/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12303 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10441. Description: sIs10441 [rCes C03A3.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12304 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10289. Description: sIs10289 [rCesB0545.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Simon Wong Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12310 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12310. Description: sEx12310 [rCesR05G6.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Emily Ha Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12312 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12312. Description: sEx12312[rCesK12C11.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Emily Ha Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12315 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12315. Description: sEx12315 [rCesK10B3.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Emily Ha Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12319 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12319. Description: sEx12319 [rCes F13G3.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12326 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12326. Description: sEx12326 [rCes Y73F4A.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12345 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12345. Description: sEx12345 [rCesC06E8.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12350 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12119. Description: sIs12119 [rCesC53A3.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Anthony Lee Received: 03/23/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12351 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12121. Description: sIs12121 [rCes F43G9.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12352 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12144. Description: sIs12144 [rCesR08E3.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Anthony Lee Received: 02/08/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12357 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12078. Description: sIs12078 [rCes T08G11.1b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12358 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12098. Description: sIs12098 [rCesC05B5.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Simon Wong Received: 10/20/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12360 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12140. Description: sIs12140 [rCes M195.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12361 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12069. Description: sIs12069 [rCesR05F9.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 11/14/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12362 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12101. Description: sIs12101[rCesF42A9.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 12/17/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12364 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10569. Description: sIs10569 [rCes C05D11.12::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12365 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12365. Description: sEx12365 [rCes C47E12.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12367 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12367. Description: sEx12367 [rCes ZK593.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12371 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12371. Description: sEx12371 [rCes F40F11.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12372 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12372. Description: sEx12372[rCesF26F4.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12378 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12378. Description: sEx12378 [rCes C45G9.10a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12380 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13480. Description: sEx13480 [rCes F58B3.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: xx Made by: Allan Mah Received: 05/05/09 University of British Columbia ----------------------------------------------------------------------------- Strain: BC12382 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12382. Description: sEx12382 [rCesC35D10.12::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/17/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12383 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12383. Description: sEx12383 [rCes C35D10.12::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12384 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12384. Description: sEx12384 [rCesC35D10.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 03/02/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12385 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12385. Description: sEx12385 [rCes F26A1.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12386 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12386. Description: sEx12386 [rCes F26A1.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12389 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12389. Description: sEx12389 contains [rCesF26A1.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 03/03/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC124 Species: Caenorhabditis elegans Genotype: dpy-14(e188) unc-13(e51) unc-37(s80)/unc-15(e73) I. Description: Heterozygotes are WT and segregate more WT, paralyzed Unc and DpyUncLet. The DpyUncLet larvae are abnormal. Pick WT to maintain. Mutagen: Outcrossed: x Made by: Received: 09/01/82 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12400 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15005. Description: sEx12400 [rCesF45E1.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). [NOTE: (07/15/2019) This strain was incorrectly annotated as carrying sEx15005. The correct transgene name is sEx12400.] Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/23/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12401 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10527. Description: sIs10527 [rCes ZK637.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12407 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12407. Description: sEx12407 [rCesT04A8.14::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12408 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12408. Description: sEx12408 [rCes T04A8.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12414 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12414. Description: sEx12414 [rCesF10F2.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/11/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12415 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10601. Description: sIs10601[rCesK08E3.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Simon Wong Received: 06/19/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12416 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10631. Description: sIs10631 [rCes Y39B6A.35::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12417 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10638. Description: sIs10638 [rCes Y43F4A.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12418 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12097. Description: sIs12097 [rCes T10F2.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12419 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10629. Description: sIs10629 [rCesY39A1A.15b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 05/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12420 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12076. Description: sIs12076 [rCesC34F11.9a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 04/03/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12421 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12421. Description: sEx12421[rCesY49E10.6 + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 06/04/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12422 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12422. Description: sEx12422 [zip-8a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 12/18/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12423 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12423. Description: sEx12423 [rCes F23F12.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12430 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12430. Description: sEx12430 [rCes C56G2.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12431 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12431. Description: sEx12431 [rCesC09E7.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 04/23/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12436 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12436. Description: sEx12436 [rCes M88.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12438 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12438. Description: sEx12438 [rCes R07E5.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12442 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12442. Description: sEx12442[rCes R07E5.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/08/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12457 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12457. Description: sEx12457 [rCes F54D8.6a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12466 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12466. Description: sEx12466 [rCes F35G12.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12468 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12468. Description: sEx12468 [rCesF56F3.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/20/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12469 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10796. Description: sIs10796 [rCes ZC395.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 03/15/25 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12473 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11776. Description: sIs11776 [rCesC10H11.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Simon Wong Received: 11/10/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12475 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11667. Description: sIs11667 [rCesW05E10.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12476 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11659. Description: sIs11659 [rCes M106.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12478 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10597. Description: sIs10597 [rCes F35A5.8a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12480 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12480. Description: sEx12480 [rCes C23G10.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12482 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12482. Description: sEx12482 [rCes C18F10.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12485 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12485. Description: sEx12485 [rCes T12A2.16a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12486 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12486. Description: sEx12486 [rCesC50C3.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 05/23/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12487 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12487. Description: sEx12487 [rCesC50C3.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 09/07/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12489 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12489. Description: sEx12489 [rCes D2007.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12491 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12491. Description: sEx12491 [rCes K06H7.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12493 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12493. Description: sEx12493 [rCes K06H7.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12494 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12494. Description: sEx12494 [rCes K12H4.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC125 Species: Caenorhabditis elegans Genotype: dpy-14(e188) unc-13(e51) let-79(s81)/unc-15(e73) I. Description: Heterozygotes are WT and segregate more WT, paralyzed Unc and DpyUncLethal. The DpyUncs are abnormal larvae that die in early larval development. Pick WT to maintain. Note 5/92: probably has lost dpy-14. Mutagen: EMS Outcrossed: x Made by: Ann Rose Received: 09/01/82 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12501 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12501. Description: sEx12501 [rCes F26F4.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/25/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12510 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12510. Description: sEx12510 [rCesT10B5.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 12/16/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12513 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11146. Description: sIs11146 [rCes F40F9.6a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12514 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10566. Description: sIs10566 [rCes Y73B6BL.19::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12515 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11147. Description: dpy-5(e907)/dpy-5(e907); sIs11147 [rCes F54D8.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Simon Wong Received: 10/07/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12517 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10541. Description: sIs10541[rCesF56E3.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Anthony Lee Received: 02/26/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12523 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12523. Description: sEx12523[rCesF54H12.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12526 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10120. Description: sIs10120 [rCesC36E8.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-rays Outcrossed: x0 Made by: Anthony Lee Received: 04/20/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12531 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12531. Description: sEx12531 [rCes F17C8.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12533 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12533. Description: sEx12533 [rCes F17C8.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12534 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12534. Description: sEx12534 [rCesC38D4.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12535 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12535. Description: sEx12535 [rCesF26A1.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12540 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12540. Description: sEx12540 [rCes C45G9.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12544 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10314. Description: sIs10314 [rCesC06B3.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12550 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12550. Description: sEx12550 [rCes C08C3.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 01/10/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12551 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12551. Description: sEx12551 [rCes ZK652.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12557 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12557. Description: sEx12557 [rCes T04A6.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12579 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12579. Description: sEx12579 [rCesF56C9.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 10/24/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12584 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12584. Description: sEx12584 [rCesT20H4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12591 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12591. Description: sEx12591[rCesT05C12.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 04/03/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12592 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11722. Description: sIs11722[rCesF19C6.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Simon Wong Received: 01/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12595 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12125. Description: sIs12125 [rCesAH6.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Anthony Lee Received: 07/24/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12597 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12174. Description: sIs12174 [rCes C47A10.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12601 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12601. Description: sEx12601[rCesF44B9.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 05/22/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12604 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12604. Description: sEx12604 [rCes F44B9.3a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12608 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11790. Description: sIs11790 [rCes Y50D7A.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12609 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12449. Description: sIs12449 [rCesH38K22.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Simon Wong Received: 10/15/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12610 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx11321. Description: sEx11321[rCesT23G5.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12611 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11096. Description: sIs11096 [rCes T25G12.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12615 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10898. Description: sIs10898 [rCes C26D10.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12616 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12377. Description: sIs12377 [rCes C45G9.13::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12624 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12624. Description: sEx12624 [ceh-23::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 10/09/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12648 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11686. Description: sIs11686 [rCesF42G9.9a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Simon Wong Received: 05/02/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12649 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10739. Description: sIs10739 [rCesC51E3.7a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Simon Wong Received: 08/03/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12652 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11234. Description: sIs11234 [rCesC02E11.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Simon Wong Received: 04/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12660 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12660. Description: sEx12660 [rCes R13A5.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12665 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11849. Description: sIs11849 [rCesC30F12.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12667 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11834. Description: sIs11834 [rCesM176.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Simon Wong Received: 02/22/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12668 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12029. Description: sIs12029 [rCesT08H4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Simon Wong Received: 06/19/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12669 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10522. Description: sIs10522[rCesC17G10.2::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12670 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11830. Description: sIs11830 [rCes F55F3.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-rays Outcrossed: x Made by: Simon Wong Received: 01/10/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12673 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11859. Description: sIs11859 [rCesC53D6.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lesley Chen Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12674 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10520. Description: sIs10520 [rCesF08F1.7::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12677 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11111. Description: sIs11111[rCesC32F10.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Anthony Lee Received: 10/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12678 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11921. Description: sIs11921[rCesJC8.10a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Anthony Lee Received: 02/12/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12679 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11724. Description: sIs11724 [rCesF36H1.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Anthony Lee Received: 12/01/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12680 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11843. Description: sIs11843 [rCes Y48E1B.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12686 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12686. Description: sEx12686 [rCes F07F6.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12687 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12687. Description: sEx12687 [rCesY49A3A.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 02/18/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12688 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11135. Description: sIs11135 [rCesF15A2.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Simon Wong Received: 09/20/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12690 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12454. Description: sIs12454 [rCesF43C1.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: John Lin Received: 11/06/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12693 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12693. Description: sIs12693 [rCesF41E7.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X ray Outcrossed: x Made by: Simon Wang Received: 10/27/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12695 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12409. Description: sIs12409 [rCes T23F11.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12696 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12408. Description: sIs12408 [rCes T04A8.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12701 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12328. Description: sIs12328 [rCes W02C12.3d::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Robert Hollebakken Received: 01/04/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12702 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10570. Description: sIs10570 [rCesC03G5.1::GFP + pCeh361]. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-rays Outcrossed: x0 Made by: Robert Hollebakken Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12703 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11836. Description: sIs11836 [rCes T27E9.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12704 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12466. Description: sIs12466 [rCesF35G12.8::GFP + pCeh361]. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Anthony Lee Received: 03/04/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12707 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10900. Description: sIs10900 [rCesC18A3.5a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-ray Outcrossed: x0 Made by: Anthony Lee Received: 03/03/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12711 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12711. Description: sEx12711 [rCes K07E3.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12716 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12716. Description: sEx12716 [rCes Y41D4A.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12721 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12721. Description: sEx12721[rCesF55A8.2b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 04/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12723 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12723. Description: sEx12723[rCesT14G10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 02/08/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12726 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12726. Description: sEx12726 [rCes C26C6.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 01/27/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12729 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12729. Description: sEx12729 [rCesM88.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/22/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12735 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12735. Description: sEx12735 [rCes C31C9.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12737 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12737. Description: sEx12737 [rCesD2024.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12743 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12743. Description: sEx12743[rCesC354D10.14::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 03/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12746 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12370. Description: sIs12370 [rCesF22B3.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Simon Wong Received: 05/31/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12747 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10574. Description: sIs10574 [rCesT26A5.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Simon Wong Received: 08/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12748 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10585. Description: sIs10585 [rCesC03G5.1::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12749 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12619. Description: sIs12619 [rCes C29E4.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12750 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12375. Description: sIs12375 [rCesR144.10::GFP + pCeh361]. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-rays Outcrossed: x0 Made by: Simon Wong Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12752 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11234. Description: sIs11234 [rCesC02E11.1a::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12753 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12371. Description: sIs12371[rCesF40F11.2::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12754 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12567. Description: sIs12567 [rCesC07H6.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12755 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12541. Description: sIs12541[rCesR144.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-rays Outcrossed: x0 Made by: Robert Hollebakken Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12758 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12445. Description: sIs12445 [rCesF35G12.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-rays Outcrossed: x Made by: Robert Hollebakken Received: 01/20/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12759 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10744. Description: sIs10744 [rCesC48A7.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 04/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12760 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12629. Description: sIs12629 [rCesZK112.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 05/02/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12762 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11268. Description: sIs11268 [rCesM03F4.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Anthony Lee Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12763 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11131. Description: sIs11131 [rCesF52B5.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Anthony Lee Received: 09/20/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12764 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11131. Description: sIs11131 [rCes F41G3.12::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12765 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12335. Description: sIs12335 [rCes R151.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12766 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11844. Description: sIs11844 [rCes F18F11.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12767 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11856. Description: sIs11856 [rCes CD4.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12772 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12772. Description: sEx12772[rCesF08C6.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 05/25/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12775 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12775. Description: sEx12775 [rCesF28F9.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 01/20/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12777 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12777. Description: sEx12777 [rCesF53H8.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 01/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12778 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12778. Description: sEx12778 [rCesC26C6.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 02/08/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12784 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11831. Description: sIs11831[rCesK11D2.2::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12786 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11990. Description: sIs11990 [rCesY106G6E.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Simon Wong Received: 01/24/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12789 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11174. Description: sIs11174 [rCesT01C8.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12790 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10518. Description: sIs10518 [rCes Y17G7B.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-rays Outcrossed: x0 Made by: John Lin Received: 01/10/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12791 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12533. Description: sIs12533 [rCes F17C8.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12792 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12792. Description: sIs12792[rCesF41E7.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X ray Outcrossed: x Made by: Anthony Lee Received: 10/27/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12794 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11178. Description: sIs11178 [rCesR166.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Gamma Outcrossed: x0 Made by: Anthony Lee Received: 04/22/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12795 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12530. Description: sIs12530 [rCes C45G9.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12796 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12796. Description: sEx12796 [rCesB0336.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 05/31/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12798 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12798. Description: sEx12798 [rCes ZK470.5a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12801 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12801. Description: sEx12801 [rCes B0303.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12803 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12803. Description: sEx12803 [rCes Y18D10A.6a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12805 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12805. Description: sEx12805 [rCes Y39A1A.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12810 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12810. Description: sEx12810 [rCes R144.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Domena Tu Received: 10/01/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1282 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-62(s472) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. Unc-62 is a recessive lethal. Mutagen: No mutagen used-control screen Outcrossed: x Made by: R. Rosenbluth Received: 07/01/85 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12827 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12827. Description: sEx12827 [rCes K03B8.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1283 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) sDf20/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Gamma Rays Outcrossed: x Made by: R. Rosenbluth Received: 10/01/85 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12833 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12501. Description: sIs12501 [rCes F26F4.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12834 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11238. Description: sIs11238 [rCesC28H8.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-ray Outcrossed: x0 Made by: Simon Wong Received: 04/06/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12835 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11012. Description: sIs11012 [rCes ZK384.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12836 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11268. Description: sIs11268 [rCesM03F4.7::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12838 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12375. Description: sIs12375 [rCes R144.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12839 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12655. Description: sIs12655 [rCesF48F7.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Gamma radiation Outcrossed: x0 Made by: Robert Hollebakken Received: 03/15/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12840 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10927. Description: sIs10927 [rCesC29E4.8::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12842 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12661. Description: sIs12661[rCesR13A5.9::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12843 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11286. Description: sIs11286 [rCesK07H8.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Anthony Lee Received: 03/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12844 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12662. Description: sIs12662 [rCes F53F10.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12845 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12541. Description: sIs12541[rCesR144.11::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12846 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12642. Description: sIs12642 [rCes F08F8.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12847 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10473. Description: sIs10473 [rCes C30H6.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12848 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11450. Description: sIs11450 [rCesK08C7.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Anthony Lee Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12849 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12328. Description: sIs12328 [rCes W02C12.3d::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12850 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11836. Description: sIs11836 [rCesT27E9.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12851 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10570. Description: sIs10570 [rCesC03G5.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12852 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12852. Description: sEx12852[rCesT16G1.8::GFP + pCeh361].. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 09/08/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12856 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12856. Description: sEx12856 [rCes K10C2.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 01/10/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1287 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf26/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Gamma Rays Outcrossed: x Made by: Received: 07/01/85 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12877 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12877. Description: sEx12877 [rCes Y55F3AM.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12878 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12878. Description: sEx12878 [rCesY37A1B.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 10/28/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12879 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12879. Description: sEx12879 [rCes Y55F3AM.3b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12881 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12881. Description: sEx12881 [rCes ZK512.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/28/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12883 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12883. Description: sEx12883 [rCes F11E6.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12884 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12884. Description: sEx12884 [rCes F11E6.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12886 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12886. Description: sEx12886 [rCes ZK643.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12889 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12639. Description: sIs12639 [rCes C07H6.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1289 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf28 unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Gamma Rays Outcrossed: x Made by: R. Rosenbluth Received: 07/01/85 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12890 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11337. Description: sIs11337 [rCesY37A1B.5::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12891 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11014. Description: sIs11014 [rCes F25B4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12892 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11756. Description: sIs11756 [rCesZC373.5::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12893 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11050. Description: sIs11050 [rCesF22F7.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-rays Outcrossed: x Made by: Robert Hollebakken Received: 01/20/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12895 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12655. Description: sIs12655 [rCesF48F7.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12896 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11718. Description: sIs11718 [rCes T27F6.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12897 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12060. Description: sIs12060 [rCesZK430.2::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12898 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11074. Description: sIs11074 [rCesC47B2.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Anthony Lee Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12899 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12687. Description: sIs12687 [rCes Y49A3A.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12900 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11600. Description: sIs11600 [rCesE03G2.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Anthony Lee Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12902 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12902. Description: sEx12902 [rCes F56F3.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12907 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10558. Description: sIs10558 [rCes T09B4.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12908 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12631. Description: sIs12631 [rCes ZK112.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12909 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12641. Description: sIs12641[rCesF08F8.9a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Simon Wong Received: 10/15/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1291 Species: Caenorhabditis elegans Genotype: let-93(s734) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, UncLet and dead eggs. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Leo Donati Received: 12/12/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12912 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12716. Description: sIs12716 [rCes Y41D4A.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12913 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10486. Description: sIs10486 [rCesZK721.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Bombardment Outcrossed: x0 Made by: Robert Hollebakken Received: 07/02/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12914 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10939. Description: sIs10939 [rCes F47B3.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Bombardment Outcrossed: x0 Made by: Robert Hollebakkan Received: 08/10/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12915 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11033. Description: sIs11033[rCesF23B12.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakkan Received: 03/16/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12917 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12341. Description: sIs12341[rCesB0280.7::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12918 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11087. Description: sIs11087 [rCesF56A12.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-rays Outcrossed: x0 Made by: Robert Hollebakken Received: 04/20/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12919 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10987. Description: sIs10987 [rCesD1054.15::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-ray Outcrossed: x0 Made by: John Lin Received: 03/16/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12921 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10729. Description: sIs10729 [rCes T12G3.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Anthony Lee Received: 02/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12923 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12801. Description: sIs12801 [rCes B0303.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12924 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12798. Description: sIs12798 [rCesZK470.5a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Anthony Lee Received: 06/23/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12925 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10312. Description: sIs10312[rCesC05D11.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Anthony Lee Received: 02/23/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12926 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12782. Description: sIs12782 [rCes K04D7.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12927 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12803. Description: sIs12803 [rCesY18D10A.6a::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12933 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12933. Description: sEx12933[rCesK08F8.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12934 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12169. Description: sIs12169 [rCesT19D2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Simon Wong Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12935 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11974. Description: sIs11974 [rCes C27D6.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12941 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12941. Description: sEx12941 [rCes F56D1.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12944 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12944. Description: sEx12944 [rCes T26E3.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12952 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12952. Description: sIs12952 [rCes ZK112.1::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Simon Wong Received: 01/19/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12954 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12954. Description: sIs12954 [rCesC35D10.14::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12956 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12739. Description: sIs12739 [rCesF25G6.5::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12963 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12963. Description: sEx12963[rCesF09D12.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 03/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12967 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12797. Description: sIs12797 [rCesY57G11C.20::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with low intensity GFP expression in larval and adult. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12969 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12774. Description: sIs12774 [rCesF52H2.5::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12970 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11293. Description: sIs11293 [rCes Y71H10B.1b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12986 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12773. Description: sIs12773 [rCesF09C3.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-ray Outcrossed: x0 Made by: Simon Wong Received: 03/16/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12987 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12810. Description: sIs12810 [rCes R144.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12988 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12777. Description: sIs12777 [rCesF53H8.2::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC12989 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12989. Description: sEx12989 [rCes F10E9.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12991 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx12991. Description: sEx12991 [rCes E02D9.1b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12993 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11169. Description: sIs11169 [rCes C33H5.18a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12994 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12736. Description: sIs12736 [rCesC35B1.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Gamma Outcrossed: x0 Made by: Simon Wong Received: 04/22/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12995 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11076. Description: sIs11076 contains [rCes C47B2.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Simon Wong Received: 10/01/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12996 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10871. Description: sIs10871 [rCes ZK829.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12997 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12740 . Description: sIs12740 [rCes T04A8.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC12998 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10951. Description: sIs10951[rCesK03A11.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: John Lin Received: 04/05/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13002 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10510. Description: sIs10510 [rCes D2013.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13003 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12880. Description: sIs12880 [rCesC26F1.5::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages, but rapid decrease in adults. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC13008 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13008. Description: sEx13008 [rCesH22D14.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 07/23/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13013 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13013. Description: sEx13013 [rCes R01H10.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13032 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13032. Description: sEx13032 [rCes Y97E10AR.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13035 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13035. Description: sEx13035 [rCes K07G5.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13037 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13037. Description: sEx13037 [rCesDY3.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 01/21/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13049 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13049. Description: sEx13049 [rCes Y39A1C.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13051 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13051. Description: sEx13051 [rCes F29D11.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13072 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13072. Description: sEx13072[rCesVF11C1L.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 02/13/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13073 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12930. Description: sIs12930 [rCes F56F10.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13074 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12775. Description: sIs12775 [rCesF28F9.1::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC13075 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13075. Description: sEx13075 [rCesC53C11.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/23/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13081 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12018. Description: sIs12018 [rCesB0228.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lesley Chen Received: 11/27/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13083 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12735. Description: sIs12735 contains [rCes C31C9.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Robert Hollebakken Received: 10/21/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13088 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13088. Description: sEx13088 [rCes E02D9.1b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13105 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13105. Description: sEx13105 [rCes Y37E3.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13132 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13132. Description: sEx13132[rCesH22D14.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 07/23/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13135 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13135. Description: sEx13135 [rCesW05B2.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/23/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13136 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13136. Description: sEx13136 [rCes C27D6.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13142 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13142. Description: sEx13142 [rCes T01A4.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13144 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13144. Description: sEx13144 [rCes F21D5.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13145 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13145. Description: sEx13145 [rCesF09E5.15::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/22/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13147 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13147. Description: sEx13147 [rCes W08E3.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13149 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13149. Description: sEx13149 [rCes F15G9.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13170 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12260. Description: sIs12260 [rCesF25H2.1::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC13172 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12730. Description: sIs12730 [rCesM88.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 05/22/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13176 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13176. Description: sEx13176 [rCes Y66D12A.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13183 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13183. Description: sEx13183 [rCes H01G02.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13193 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13193. Description: sEx13193 [rCes F11C1.5a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13200 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13200. Description: sEx13200 [rCesZK520.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13201 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13201. Description: sEx13201 [rCes F01F1.15::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13219 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12966. Description: sIs12966 [rCesW05E7.3::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC13222 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12984. Description: sIs12984 [rCesY75B8A.20::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 02/08/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13227 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13227. Description: sEx13227 [rCes K08E7.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13241 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13241. Description: sEx13241[rCesY22F5A.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/12/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13243 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13243. Description: sEx13243 [rCes Y56A3A.18::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13244 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13244. Description: sEx13244 [rCesT27A1.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13248 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12963. Description: sIs12963 [rCesF09D12.1::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: John Lin Received: 04/27/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13250 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13000. Description: sIs13000 [rCesT27F7.3b::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC13253 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13253. Description: sEx13253 [rCesY54E2A.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 07/31/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13255 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13255. Description: sEx13255 [rCesT02C5.5b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 10/20/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13256 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13256. Description: sEx13256 [rCes Y37D8A.17::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13258 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13258. Description: sEx13258 [rCesZK524.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 04/02/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13261 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13261. Description: sEx13261[rCesF32A6.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13263 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13263. Description: sEx13263 [rCes F25B4.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13266 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13266. Description: sEx13266 [rCesC04E12.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 03/06/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13283 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12964. Description: sIs12964 [rCesY87G2A.15::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC13287 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13287. Description: sEx13287 [rCes C36E8.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13292 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13292. Description: sEx13292[rCesC02C6.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/11/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13303 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12531. Description: sIs12531 [rCes F17C8.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13305 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12563. Description: sIs12563 [rCesR13A5.6::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 02/08/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13306 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12555. Description: sIs12555 [rCes T04A6.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13307 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12976. Description: sIs12976 [rCes C34B2.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13308 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12201. Description: sIs12201[rCesR07B7.11::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC13309 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13309. Description: sEx13309 [rCesF35G12.12::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Zhongying Zhao Received: 06/19/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13310 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13310. Description: sEx13310 [rCesM04F3.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 03/24/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13313 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13313. Description: sEx13313 [rCesF25H8.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/08/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13318 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13318. Description: sEx13318 [rCesC04C3.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525).. Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13333 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13333. Description: sEx13333 contains [rCes Y48C3A.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Domena Tu Received: 10/01/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13335 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13109. Description: sIs13109 [rCesY73B6BL.30::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-rays Outcrossed: x0 Made by: Lesley Chen Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC13336 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13113. Description: sIs13113 [rCesY110A7A.20::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC13337 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12928. Description: sIs12928 [rCesY39B6A.19::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 06/07/13 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13348 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13348. Description: sEx13348 [rCesK08F4.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/16/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13350 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13350. Description: sEx13350 [rCes R74.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13354 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11457. Description: sIs11457 [rCesR10H10.5::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC13361 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13361. Description: sEx13361[rCesH09G03.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 01/03/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13365 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13365. Description: sEx13365 [rCesC54D10.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 11/06/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13370 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13370. Description: sEx13370 [rCes C27H6.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13371 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13371. Description: sEx13371[rCesC29E6.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 07/18/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13375 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13375. Description: sEx13375 [rCesY53C12B.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13378 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13378. Description: sEx13378 [rCes h06h21.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/21/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13380 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13380. Description: sEx13380 [rCes W08D2.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13391 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13391. Description: sEx13391[rCesC09D8.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 04/06/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13401 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12199. Description: sIs12199 [rCes T26E3.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13403 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13403. Description: sEx13403[rCesF55C7.7a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 01/31/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13412 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13412. Description: sEx13412 [rCes Y105E8A.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13418 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13418. Description: sEx13418 [rCesT26A5.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Domena Tu Received: 10/28/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13422 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13422. Description: sEx13422 [rCes T11F9.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13429 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13429. Description: sEx13429 [rCes R11A8.7a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13430 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13430. Description: sEx13430 [rCesC52E12.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13431 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13431. Description: sEx13431 [rCes F38E9.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13436 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13436. Description: sEx13436 [rCesT08B2.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Zhongying Zhao Received: 04/10/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13443 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13443. Description: sEx13443 [rCesF36H2.3a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13446 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13446. Description: sEx13446 [rCes Y44A6B.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13447 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13447. Description: sEx13447 [rCesC11E4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/17/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13455 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13455. Description: sEx13455 [rCes F58G4.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13458 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13458. Description: sEx13458 [rCes F10A3.13::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13459 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13459. Description: sEx13459 [rCesY22D7AL.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 04/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13460 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13460. Description: sEx13460 [rCes F46C5.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13467 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13467. Description: sEx13467 [rCes T23F4.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13470 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13470. Description: sEx13470 [rCes K03B8.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13471 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13471. Description: sEx13471[rCesT04G9.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/08/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13472 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13472. Description: sEx13472 [rCes F31C3.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13480 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13480. Description: sEx13480 [rCes F58B3.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13483 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13483. Description: sEx13483[rCesW06H8.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/12/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13488 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13488. Description: sEx13488 [rCesF31B12.1b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 10/20/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13491 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12001. Description: sIs12001 [rCes T06G6.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13494 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13494. Description: sEx13494 [rCesC27A7.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 10/17/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13495 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13495. Description: sEx13495 [rCesF47B10.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/02/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13501 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13501. Description: sEx13501[rCesK01C8.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/25/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13503 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13503. Description: sEx13503[rCesF58H1.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 10/15/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13515 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13209. Description: sIs13209 [rCesF41E6.13a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lesley Chen Received: 02/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13516 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13245. Description: sIs13245 [rCes F42E11.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13517 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11997. Description: sIs11997 [rCes F49E12.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13518 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13281. Description: sIs13281 contains [rCes C09G12.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Robert Hollebakken Received: 10/01/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13519 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13260. Description: sIs13260 [rCes T19A5.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13520 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13244. Description: sIs13244 [rCesT27A1.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Robert Hollebakken Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13533 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11984. Description: sIs11984 [rCesC50E10.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Edmund Tang Received: 07/23/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13535 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13247. Description: sIs13247 [rCesC40C9.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Edmund Tang Received: 10/28/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13539 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13539. Description: sEx13539 [rCesT04D3.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 03/15/25 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13544 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13544. Description: sEx13544 [rCesC09E10.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 10/11/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13545 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13645. Description: sEx13645 [rCesC04F12.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13557 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13557. Description: sEx13557 [rCesT12D8.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/22/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13558 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13558. Description: sEx13558 [rCesF41C6.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13560 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13559. Description: sIs13559 contains [rCesH27M09.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Marco Gallo Received: 01/19/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13565 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13565. Description: sEx13565 [rCes Y95B8A.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13567 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13567. Description: sEx13567 [rCesC32D5.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/17/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13568 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13568. Description: sEx13568 [rCesT12D8.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13574 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13574. Description: sEx13574 [rCes T09A12.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13583 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10487. Description: sIs10487 [rCesB0304.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lesley Chen Received: 05/22/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13587 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10381. Description: sIs10381 [rCesF43G9.1::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Edmund Tang Received: 02/19/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13595 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13595. Description: sEx13595 [rCesW03F9.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Replaces strain BC11329. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/02/14 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13600 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13600. Description: sEx13600 [rCes K11G12.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13603 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13603. Description: sEx13603[rCesZK512.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/21/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13607 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13607. Description: sEx13607 [rCesB0403.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/22/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13608 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13608. Description: sEx13608 [rCesC52B11.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 03/24/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13611 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13611. Description: sEx13611 [rCes M04C9.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13616 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13137. Description: sIs13137 [rCes C43C3.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13623 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13623. Description: sEx13623 [rCes K07C11.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13624 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13624. Description: sEx13624 [rCes Y71F9B.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13627 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13627. Description: sEx13627 [rCes T10F2.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13632 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13632. Description: sEx13632[rCesF55H2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 04/16/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13635 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13635. Description: sEx13635 [rCes T28D6.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13637 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13637. Description: sEx13637 [rCes Y71F9AL.14::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13643 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13643. Description: sEx13643 [rCes C04A11.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13645 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13645. Description: sEx13645 [rCesC04F12.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 10/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13653 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13653. Description: sEx13653 [rCes W06H3.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13657 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13657. Description: sEx13657 [rCesY66H1B.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/08/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13658 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13658. Description: sEx13658 [rCesW09C5.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 09/13/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13659 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13659. Description: sEx13659 [rCesF37H8.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Received: 08/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13660 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13660. Description: sEx13660 [rCesF37H8.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13667 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13264. Description: sIs13264 [rCes F25B4.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13672 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13672. Description: sEx13672 [rCesY105E8A.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 11/04/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13675 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13675. Description: sEx13675 [rCes F41E6.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13679 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13679. Description: sEx13679 [rCesC07D10.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/08/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13683 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13683. Description: sEx13683[rCesF40E10.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/11/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13686 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13686. Description: sEx13686 [rCesR11.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 12/01/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13691 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13691. Description: sEx13691[rCesF16A11.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13693 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13693. Description: sEx13693 [rCes ZK256.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 01/10/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13697 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13697. Description: sEx13697 [rCesY48G8AL.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/22/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13699 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13699. Description: sEx13699 [rCesZC116.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/23/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13701 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13701. Description: sEx13701 [rCes C02H7.3a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/25/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13702 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13702. Description: sEx13702 [rCes F14B4.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13705 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13705. Description: sEx13705 [rCesR06B10.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 01/20/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13706 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13706. Description: sEx13706 [rCesC53A5.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 07/03/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13708 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13708. Description: sEx13708 [rCesF28C12.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 04/03/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13711 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13711. Description: sEx13711 [rCes F28C12.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13714 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13714. Description: sEx13714 [rCes C08F8.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13719 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13291. Description: sIs13291 [rCes F48E3.3::GFP + pCeh361] Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-rays Outcrossed: x Made by: Lesley Chen Received: 01/17/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13725 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13725. Description: sEx13725 [rCes W02A11.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13726 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13726. Description: sEx13726 [rCes Y111B2A.18::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13727 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13727. Description: sEx13727 [rCes y113g7b.17::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13728 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13215. Description: sIs13215 [rCes Y48A6B.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13729 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12212. Description: sIs12212[rCesAH6.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Edmund Tang Received: 12/22/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13735 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13735. Description: sEx13735 [rCesF55C10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13737 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13737. Description: sEx13737 [rCesC44H4.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Allan Mah Received: 06/19/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13739 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13739. Description: sEx13739 [rCesC15F1.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 04/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13743 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13743. Description: sEx13743[rCesT19B10.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/03/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13747 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10161. Description: sIs10161[rCesC34E10.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lesley Chen Received: 04/10/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13748 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12817. Description: sIs12817 [rCesB0393.1::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/10/05 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC13749 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12251. Description: sIs12251 [rCes R144.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13750 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12127. Description: sIs12127 [rCesAH6.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lesley Chen Received: 01/24/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13756 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13756. Description: sEx13756 [rCesZK370.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 04/22/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13757 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13757. Description: sEx13757 [rCes Y18D10A.6b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13758 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10944. Description: sIs10944 [rCes F40F8.1b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13759 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13263. Description: sIs13263 [rCes F25B4.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13765 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13765. Description: sEx13765 [rCesF47F6.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/07/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13769 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13769. Description: sEx13769 [rCesF54F2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13771 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13771. Description: sEx13771[rCesC24A8.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 04/24/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13773 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13773. Description: sEx13773[rCesF58G1.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/20/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13775 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13775. Description: sEx13775 [rCes F01G4.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13776 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13776. Description: sEx13776 [rCes Y57A10A.23::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13780 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13780. Description: sEx13780 [rCes F36F2.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 08/29/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13784 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13784. Description: sEx13784 [rCesF47D12.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 05/22/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13785 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13785. Description: sEx13785 [rCes Y69A2AR.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13786 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13786. Description: sEx13786 [rCesF56C11.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Zhongying Zhao Received: 07/05/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13793 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13793. Description: sEx13793 [rCesY54G2A.29::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/07/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13802 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13802. Description: sEx13802[rCesT21C12.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/02/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13809 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13297. Description: sIs13297 [rCes T27B1.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-ray Outcrossed: x0 Made by: Lesley Chen Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1381 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) sDf29/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Gamma Rays Outcrossed: x Made by: R. Rosenbluth Received: 08/01/84 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13810 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12977. Description: sIs12977 [rCesR10E11.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: John Lin Received: 01/24/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13815 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13815. Description: sEx13815 [rCesR107.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13816 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13816. Description: sEx13816 contains [rCes R107.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 11/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13820 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11342. Description: sIs11342[rCesC16D9.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: John Lin Received: 11/21/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13822 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13822. Description: sEx13822[rCesZC416.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 03/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13824 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13824. Description: sEx13824 [rCesF19H6.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/20/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13826 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13826. Description: sEx13826 [rCes F13D12.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13830 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13830. Description: sEx13830 [rCes F07A11.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13836 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13836. Description: sEx13836 [rCesC38H2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13840 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13840. Description: sEx13840 [rCes Y119D3B.14::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13845 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13845. Description: sEx13845 [rCes C07A9.3a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13846 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13846. Description: sEx13846 [rCesB0213.15::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 11/14/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13851 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13851. Description: sEx13851 [rCes F42G9.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13858 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13858. Description: sEx13858 [rCes C42C1.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13859 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13859. Description: sEx13859 [rCes R74.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/08/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13861 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13252. Description: sIs13252[rCesF01G12.5a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Tony Luo Received: 02/06/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13862 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13862. Description: sEx13862[rCesZC84.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 03/02/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13865 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13865. Description: sEx13865 [rCesY17G9B.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 04/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13871 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13871. Description: sEx13871 [rCes F36D4.5a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13878 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13878. Description: sEx13878 [rCesY37E11AR.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/22/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13884 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13884. Description: sEx13884 [rCes F48E3.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13886 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13886. Description: sEx13886 [rCes T01B11.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13890 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13890. Description: sEx13890 [rCes C13G3.3a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13891 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13891. Description: sEx13891[rCesC06A8.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/08/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13892 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13892. Description: sEx13892[rCesC14A4.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13894 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13894. Description: sEx13894 [rCes T11F9.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13896 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13896. Description: sEx13896 [rCesC13C4.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13897 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13897. Description: sEx13897 [rCes C11H1.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13898 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13898. Description: vsEx13898 [rCesC08B11.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13899 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13899. Description: sEx13899 [rCes F54G2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13915 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13915. Description: sEx13915 [rCes H28O16.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13916 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13916. Description: sEx13916 [rCesW09B6.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/13/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13919 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13919. Description: sEx13919 [rCes R12C12.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13923 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13923. Description: sEx13923[rCesF53A9.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/30/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13932 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13932. Description: sEx13932[rCesZK652.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/31/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13936 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13936. Description: sEx13936 [rCesT03F6.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/06/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13941 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13941. Description: sEx13941 [rCes F25H2.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13951 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13951. Description: sEx13951[rCesY54E5A.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13952 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13952. Description: sEx13952[rCesK12G11.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 09/19/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13953 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13953. Description: sEx13953 [rCes C04F12.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13954 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13954. Description: sEx13954 [rCesF10E7.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13956 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13956. Description: sEx13956 [rCesC04G6.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13961 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13961. Description: sEx13961 [rCes B0024.14b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13968 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13968. Description: sEx13968 [rCesF35H12.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13978 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13978. Description: sEx13978 [rCes F59B8.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13979 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13979. Description: sEx13979 [rCes C05D11.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13981 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13981. Description: sEx13981 [rCes C04C3.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13983 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13983. Description: sEx13983 [rCesY55F3AM.12::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/02/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13985 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13985. Description: sEx13985 [rCesC01F4.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13988 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13988. Description: sEx13988 [rCes C02F5.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13996 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13996. Description: sEx13996 [rCesC03D6.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 09/28/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC13998 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx13998. Description: sEx13998 [rCesC17H11.6b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14006 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14006. Description: sEx14006 [rCesC47D12.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/12/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14009 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14009. Description: sEx14009 [rCesF14D12.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 04/07/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14010 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14010. Description: sEx14010 [rCes F49C12.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14012 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14012. Description: sEx14012[rCesT24D11.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 07/18/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14014 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13316. Description: sIs13316 [rCes ZK353.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14016 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14016. Description: sEx14016 [rCesF54D7.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14023 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13333. Description: sIs13333 [rCes Y48C3A.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14025 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14025. Description: sEx14025 [rCesT07F10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/16/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14027 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14027. Description: sEx14027 [rCes F46F6.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14029 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14029. Description: sEx14029 [rCes C54G4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14036 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14036. Description: sEx14036 [rCesF20H11.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14038 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14038. Description: sEx14038 [rCesT26A5.5a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/16/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14044 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14044. Description: sEx14044 [rCes F40F9.6b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14050 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14050. Description: sEx14050 [rCes M01D7.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14052 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14052. Description: sEx14052[rCesK02A4.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/02/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14057 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14057. Description: sEx14057 [rCesF30A10.8a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/14/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14060 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14060. Description: sEx14060 [rCes C54D2.5a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14064 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14064. Description: sEx14064 [rCes F35H12.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14068 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14068. Description: sEx14068 [rCes M03A8.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14069 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14069. Description: sEx14069 [rCesW02H5.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 04/20/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14070 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14070. Description: sEx14070 [rCesF39C12.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14072 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14072. Description: sEx14072 [rCesF56E10.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 04/12/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14074 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14074. Description: sEx14074 [rCesY55D5A.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 08/24/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14075 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14075. Description: sEx14075 [rCes C42D8.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14084 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14084. Description: sEx14084 [rCes M106.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14088 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14088. Description: sEx14088 [rCesT11B7.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14096 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14096. Description: sEx14096 [rCes C16C10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14107 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14107. Description: sEx14107 [rCes C10G8.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14109 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14109. Description: sEx14109 [rCesC05D9.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 08/08/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14110 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14110. Description: sEx14110 [rCesC10E2.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/15/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14111 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14111. Description: sEx14111 [rCes C09D4.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14114 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14114. Description: sEx14114 [rCesY22D7AR.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 02/29/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14118 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14118. Description: sEx14118 [rCes B0546.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/05/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14119 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14119. Description: sEx14119 [rCes B0336.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14121 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14121. Description: sEx14121[rCesY116A8C.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 01/24/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14123 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14123. Description: sEx14123 [rCesY48G9A.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/12/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14126 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14126. Description: sE14126 [rCesC34D1.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). sEx14126 [rCesC34D1.3::GFP + pCeh361]. Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 03/02/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14128 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14128. Description: sEx14128 [rCes C16C10.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14129 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14129. Description: sEx14129 [rCesF20B6.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 04/18/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14130 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14130. Description: sEx14130 [rCesY69A2AR.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 08/03/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14131 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14131. Description: sEx14131 [rCes F10C1.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 12/08/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14133 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14133. Description: sEx14133[rCesB0350.2f::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 09/25/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14136 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14136. Description: sEx14136 [rCesF59G1.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 11/15/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14138 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14138. Description: sEx14138 [rCes C03B8.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14144 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14144. Description: sEx14144 [rCesT14F9.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/16/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14151 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14151. Description: sEx14151[rCesC49A1.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14153 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14153. Description: sEx14153 [rCes C34D4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14155 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14155. Description: sEx14155 contains [rCes C39F7.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 10/01/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14160 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14160. Description: sEx14160 [rCes C33A12.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14162 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14162. Description: sEx14162 [rCes C44C1.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14164 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14164. Description: sEx14164 [rCesC37C3.6b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14167 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13148. Description: sIs13148 [rCesR10E11.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Tony Luo Received: 06/08/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14172 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14172. Description: sEx14172 [rCesK08F8.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 10/20/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14175 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14175. Description: sEx14175 [rCes F32D1.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 06/07/13 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14176 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14176. Description: sEx14176 [rCesR08D7.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0. Made by: Allan Mah Received: 08/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14177 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14177. Description: sEx14177 [rCesT09B4.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14180 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13037. Description: sIs13037 [rCesDY3.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Tony Luo Received: 01/21/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14184 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14184. Description: sEx14184 [rCes F42G4.3b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14187 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14187. Description: sEx14187 [rCes W02A2.1::GFP + pCeh361] Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/17/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14193 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14193. Description: sEx14193 [rCesW09G12.4::GFP + pCeh361] Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/29/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14196 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14196. Description: sEx14196 [rCes ZK370.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14199 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12965. Description: sIs12965 [rCesT01B10.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Tony Luo Received: 10/28/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14200 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12234. Description: sIs12234 [rCesC13G5.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Tony Luo Received: 08/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14203 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14203. Description: sEx14203[rCesF02E9.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14205 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14205. Description: sEx14205 [rCes D1046.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14206 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14206. Description: sEx14206 [rCesF33A8.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/04/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14211 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14211. Description: sEx14211 [rCes F25B5.6a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14214 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14214. Description: sEx14214 [rCes F10E9.6a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 01/10/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14216 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14216. Description: sEx14216 [rCesF26F12.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14220 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14220. Description: sEx14220 [rCesF09D1.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 09/08/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14221 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14221. Description: sEx14221 [rCes F15D3.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14222 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14222. Description: sEx14222 [rCes F33H2.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14224 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14224. Description: sEx14224 [rCesF18A1.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/05/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14225 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14225. Description: sEx14225 [rCesF21F8.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/13/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14226 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14226. Description: sEx14226 [rCes F25D1.1::GFP + pCeh361]. F25D1.1 is ppm-1.A Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14228 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14228. Description: sEx14228 [rCes F35F11.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14229 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14229. Description: sEx14229 [rCesT10H4.12::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/06/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14231 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14231. Description: sEx14231 [rCesF29F11.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/08/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14232 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14232. Description: sEx14232 contains [rCes F38A5.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 12/02/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14235 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14235. Description: sEx14235 [rCes R07H5.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14237 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14237. Description: sEx14237 [rCesC03H5.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 01/04/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14238 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14238. Description: sEx14238 [rCes F41C3.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14239 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12504. Description: sIs12504 [rCes F26F4.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Tony Luo Received: 08/11/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1424 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-331(s427) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Homozygous s427 is a cold sensitive sterile adult.(??) Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Rosenbluth R Received: 11/13/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14240 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10142. Description: sIs10142[rCesY54G2A.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: John Lin Received: 04/03/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14242 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13607. Description: sIs13607 [rCesB0403.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Tony Luo Received: 02/22/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14244 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13698. Description: sIs13698 [rCes ZK836.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-ray Outcrossed: x0 Made by: John Lin Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14245 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14245. Description: sEx14245 [rCesF56D12.5a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/19/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14247 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14247. Description: sEx14247 [rCes F42G4.3a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14248 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14248. Description: sEx14248 [rCes F58F6.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14251 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14251. Description: sEx14251[rCes JC8.3a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/22/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14252 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14252. Description: sEx14252[rCesH19N07.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/20/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14255 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14255. Description: sEx14255 [rCesC33D3.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/22/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14258 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14258. Description: sEx14258 [rCesF40F4.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 05/02/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14259 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14259. Description: sEx14259 [rCes F25E2.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/25/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14261 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14261. Description: sEx14261[rCesF38A5.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/16/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14263 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14263. Description: sEx14263 [rCes CD4.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 11/20/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14264 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14264. Description: sEx14264 [rCes R07E4.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14266 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14266. Description: sEx14266 [rCesF35E8.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/16/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14270 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14270. Description: sEx14270 [rCes F41E6.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14271 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14271. Description: sEx14271 [rCes F38E9.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14272 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14272. Description: sEx14272 [rCes F45E4.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14275 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14275. Description: sEx14275 [rCes F49C12.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14277 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14277. Description: sEx14277 [rCes F40G9.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14279 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14279. Description: sEx14279 [rCesF42G8.12::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/07/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14283 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14283. Description: sEx14283[rCesW02B12.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 02/20/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14284 Species: Caenorhabditis elegans Genotype: dpy-5(e907); sEx14284. Description: sEx14284 [rCesF53E4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/15/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14288 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14288. Description: sEx14288 [rCes F09G2.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14289 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14289. Description: sEx14289 [rCesF46G10.7a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/24/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14291 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14291. Description: sEx14291 [rCes D1037.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14294 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14294. Description: sEx14294 [rCesF52D10.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14295 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14295. Description: sEx14295 [rCes F59D12.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14300 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14300. Description: sEx14300 [rCes Y70C5C.6a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/30/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14308 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14308. Description: sEx14308 [rCesZK1127.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14309 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14309. Description: sEx14309 [rCesF30F8.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/06/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14311 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14311. Description: sEx14311[rCesT23G7.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 04/23/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14312 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14312. Description: sEx14312 [rCes E04D5.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14315 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14315. Description: sEx14315 [rCes T05H10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14317 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14317. Description: sEx14317 [rCes C46C11.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14319 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13429. Description: sIs13429 [rCes R11A8.7a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14320 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13711. Description: sIs13711[rCesF28C12.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: John Lin Received: 06/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14321 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11063. Description: sIs11063[rCesF38A5.13::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: J Lin / L Halim Received: 10/20/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14322 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14322. Description: sEx14322 [rCes R03D7.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14325 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14325. Description: sEx14325 [rCesR05G6.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14328 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14328. Description: sEx14328 [rCes F45E6.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1433 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-345(s578) unc-46(e177)/eT1 V. Description: WT strain which segregates WT, Unc-36, dead eggs, and DpyUncLets. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: R. Rosenbluth Received: 02/22/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14330 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14330. Description: sEx14330 [rCesC44H4.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 06/19/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14332 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14332. Description: sEx14332[rCesC06G1.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14336 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14336. Description: sEx14336 [rCesC36B1.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 05/03/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14337 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14337. Description: sEx14337 [rCes F09B9.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/29/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1434 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-330(s573) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, DpyUncLet and dead eggs. Lethal mid larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: R. Rosenbluth Received: 02/03/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14340 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14340. Description: sEx14340 [rCes T21C9.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14342 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14342. Description: sEx14342[rCesY73B6BL.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/11/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14344 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14344. Description: sEx14344 [rCes K07B1.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14347 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14347. Description: sEx14347 [rCes Y48G10A.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1435 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-329(s575)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Some DpyUnc escapers are Sterile and have a protruding vulva. Mutagen: EMS Outcrossed: x Made by: Rosenbluth R Received: 11/13/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14351 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14351. Description: sEx14351 [rCes R12E2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14357 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14357. Description: sEx14357 [rCes T05H10.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14359 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14359. Description: sEx14359 [rCesT02G5.9a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14360 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14360. Description: sEx14360 [rCesT27C4.4b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 03/29/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14363 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14363. Description: sEx14363 [rCes T24H7.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14364 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11021. Description: sIs11021[rCesF28F8.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Tony Luo Received: 06/19/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14365 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs12991. Description: sIs12991 [rCes E02D9.1b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Tony Luo Received: 07/27/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14367 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14367. Description: sEx14367 [rCesT10B10.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 10/04/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14371 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14371. Description: sEx14371[rCesF45E10.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/24/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14376 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14376. Description: sEx14376 [rCesY60A3A.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14378 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14378. Description: sEx14378 [rCes F43G9.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14382 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14382. Description: sEx14382 [rCes F35D11.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14385 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14385. Description: sEx14385 [rCesF16D3.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/16/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14386 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14386. Description: sEx14386 [rCes T14D7.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14389 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14389. Description: sEx14389 [rCesC30H7.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/19/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1439 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-344(s376) unc-46(e177)/eT1 V. Description: WT strain which segregates WT, Unc-36, and dead eggs. (Egg lethal.) Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Rosenbluth R Received: 11/13/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14393 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14393. Description: sEx14393[rCesW02B12.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 04/24/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14394 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14394. Description: sEx14394 [rCes C16C10.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14395 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14395. Description: sEx14395 [rCesY17G7B.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 10/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14396 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14396. Description: sEx14396 [rCes W08F4.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14402 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14402. Description: sEx14402 [rCes Y102A11A.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14403 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14403. Description: sEx14403 [rCes T05H4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14404 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14404. Description: sEx14404 [rCes T24B8.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14406 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14406. Description: sEx14406 [rCes T14F9.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 08/25/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14409 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14409. Description: sEx14409 [rCes C14A4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14412 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14412. Description: sEx14412 [rCes Y53C10A.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14416 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14416. Description: sEx14416 [rCes Y71G12B.15::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14419 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14419. Description: sEx14419 [rCes Y73E7A.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14421 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14421. Description: sEx14421 [rCes C29H12.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14423 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14423. Description: sEx14423 [rCesM01D7.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 10/27/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14424 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14424. Description: sEx14424 [rCes Y87G2A.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14427 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14427. Description: sEx14427 [rCes F53C11.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14433 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14433. Description: sEx14433 [rCes F55C5.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14437 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14437. Description: sEx14437 [rCes Y18H1A.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14438 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14438. Description: sEx14438 [rCesF02E9.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/16/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14442 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14442. Description: sEx14442[rCesK04F10.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 08/13/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14444 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14444. Description: sEx14444 [rCes F38A1.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14448 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14448. Description: sEx14448 [rCesF17C11.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/16/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14450 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13786. Description: sIs13786 [rCes F56C11.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14451 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13955. Description: sIs13955 [rCesC50F7.4::GFP + pCeh361]. Rescued dpy-5 (WT gross phenotype) with GFP expression in all stages. Array transmission is greater than 99.5% (segregates less than 0.5% Dpys). Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Tony Luo Received: 02/19/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14452 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13799. Description: sIs13799 [rCesC27A2.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Tony Luo Received: 11/14/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14456 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14456. Description: sEx14456 [rCes C50D2.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14461 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14461. Description: sEx14461 [rCes T10C6.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14465 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14465. Description: sEx14465 [rCes C06H2.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14466 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14466. Description: sEx14466 [rCesR05H10.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 02/19/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14473 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14473. Description: sEx14473 [rCes T15H9.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14475 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14475. Description: sEx14475 [rCes F55A4.8a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/25/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14480 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14480. Description: sEx14480 [rCesY48G8AL.8a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Domena Tu Received: 01/24/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14482 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14482. Description: sEx14482[rCesY54G2A.18::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/18/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14486 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13954. Description: sIs13954 [rCesF10E7.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Tony Luo Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14487 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13738. Description: sIs13738 [rCesC18G1.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Tony Luo Received: 11/10/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14488 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13985. Description: sIs13985 [rCesC01F4.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Tony Luo Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14489 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13451. Description: sIs13451 [rCesW06D4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: John Lin Received: 11/30/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14491 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14491. Description: sEx14491 [rCes ZC247.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14493 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14493. Description: sEx14493 [rCes Y56A3A.13::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14496 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14496. Description: sEx14496 [rCes C13G3.3b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14499 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14499. Description: sEx14499 [rCesM01E11.7a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14500 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14500. Description: sEx14500 [rCesF55G1.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/21/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14501 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14501. Description: sEx14501[rCesT10C6.13::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/31/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14502 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14502. Description: sEx14502 [rCes F53C11.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14506 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14506. Description: sEx14506 [rCes F45F2.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14507 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14507. Description: sEx14507 [rCes F45F2.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14508 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14508. Description: sEx14508 [rCes C37C3.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14510 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14510. Description: sEx14510 [rCes H06H21.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14511 Species: Caenorhabditis elegans Genotype: dpy-5(e907); sEx14511. Description: dpy-5(e907); sEx14511[rCesM03A1.6b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 07/15/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14514 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14514. Description: sEx14514 [rCes Y47G6A.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14516 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14516. Description: sEx14516 [rCesT21D12.9a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/30/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14517 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14517. Description: sEx14517 [rCes T19B4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 10/11/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14519 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14519. Description: sEx14519 [rCes R74.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/08/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14524 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13981. Description: sIs13981[rCesC04C3.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Tony Luo Received: 03/16/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14526 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13038. Description: sIs13038 [rCesK03E6.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: John Lin Received: 09/11/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14531 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14531. Description: sEx14531 [rCes Y57G11C.13::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14532 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14532. Description: sEx14532 [rCes D2085.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14533 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14533. Description: sEx14533 contains [rCes Y57G11C.12::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 10/01/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14536 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14536. Description: sEx14536 [rCesF43C1.2b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/17/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14538 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14538. Description: sEx14538 [rCesW02D3.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/20/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14539 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14539. Description: sEx14539 [rCes F26H9.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14544 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14544. Description: sEx14544 [rCes T11A5.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14547 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14547. Description: sEx14547 [rCes F59F4.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14548 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14548. Description: sEx14548 [rCes Y73F8A.27::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14556 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14556. Description: sEx14556 [rCes ZK1128.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 09/01/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14561 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14561. Description: sEx14561[rCesR11A5.1b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 06/20/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14562 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14562. Description: sEx14562[rCesT11F9.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/02/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14566 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14566. Description: sEx14566 [rCesZK632.12::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/10/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14571 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14571. Description: sEx14571[rCesZK1193.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 01/25/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14574 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14574. Description: sEx14574 [rCes Y73F8A.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14575 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14575. Description: sEx14575 [rCesF37C12.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Domena Tu Received: 01/24/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14579 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13193. Description: sIs13193 [rCes F11C1.5a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14581 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14581. Description: sEx14581 [rCes C49H3.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14584 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14584. Description: sEx14584 [rCesC33D12.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/06/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14586 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14586. Description: sEx14586 [rCesK07D4.7b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Allan Mah Received: 06/19/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14588 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14588. Description: sEx14588 [rCes F37A4.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14590 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14590. Description: sEx14590 [rCesR13F6.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 11/30/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14593 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14593. Description: sEx14593 [rCesR13F6.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/08/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14596 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14596. Description: sEx14596 [rCesF28F5.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14602 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14602. Description: sEx14602 [rCes K08F11.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14605 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14605. Description: sEx14605 [rCes F45H10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14607 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14607. Description: sEx14607 [rCes K07C5.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/30/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14610 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14610. Description: sEx14610 [rCesC46H11.11b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/12/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14611 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14611. Description: sEx14611[rCesF55A12.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 02/13/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14613 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14613. Description: sEx14613[rCesW08G11.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/15/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14615 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14615. Description: sEx14615 [rCes C02F12.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14616 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14616. Description: sEx14616 [rCes F16A11.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14620 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14620. Description: sEx14620 [rCesF27C8.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/16/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14621 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14621. Description: sEx14621 [rCes F23C8.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/25/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14626 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14626. Description: sEx14626 [rCes ZK652.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14627 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14627. Description: sEx14627 [rCes F53C11.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14628 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14628. Description: sEx14628 [rCes F53C11.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14629 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14629. Description: sEx14629 contains [rCes ZK909.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 12/02/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14631 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14631. Description: sEx14631 [rCes F11A10.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14632 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14632. Description: sEx14632 [rCes F59F4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14633 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14633. Description: sEx14633 [rCes F32B6.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14634 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14634. Description: sEx14634 [rCesT02C12.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/17/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14636 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13872. Description: sIs13872[rCesB0285.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Tony Luo Received: 10/20/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14637 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13929. Description: sIs13929 [rCesT27E9.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Tony Luo Received: 02/18/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14639 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13979. Description: sIs13979 [rCes C05D11.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14642 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14642. Description: sEx14642 [rCes T07E3.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14644 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14644. Description: sEx14644 [rCes C47A10.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14646 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14646. Description: sEx14646 [rCes B0304.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14650 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14650. Description: sEx14650 [rCes F18C5.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14652 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14652. Description: sEx14652 [rCes F37C12.15::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14656 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14656. Description: sEx14656 [rCes F36D1.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14658 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14658. Description: sEx14658 [rCes C06G4.2b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14659 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14659. Description: sEx14659 [rCes Y54E10BL.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1466 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-326(s238) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, dead eggs and DpyUncLets. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: R. Rosenbluth Received: 07/01/85 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14661 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14661. Description: sEx14661[rCes F08A8.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/08/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14664 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14664. Description: sEx14664 [rCesM02G9.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/08/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14666 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14666. Description: sEx14666 [rCesF54F3.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/16/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14668 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14668. Description: sEx14668 [rCes Y57G11C.37::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 12/11/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14670 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14670. Description: sEx14670 [rCesB0272.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14671 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14671. Description: sEx14671[rCesF45H7.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Allan Mah Received: 06/19/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14672 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13936. Description: sIs13936 [rCesT03F6.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Tony Luo Received: 11/06/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14673 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13886. Description: sIs13886 [rCes T01B11.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14679 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14679. Description: sEx14679 [rCes Y32F6A.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14682 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14682. Description: sEx14682 [rCes T19A6.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14683 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14683. Description: sEx14683[rCesT25D3.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/22/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14685 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14685. Description: sEx14685 [rCesY110A7A.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 02/01/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14689 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14689. Description: sEx14689 [rCesC50B6.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/03/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14690 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14690. Description: sEx14690 [rCesZK337.1b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14694 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14694. Description: sEx14694 [rCesC17H12.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/25/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14699 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14699. Description: sEx14699 [rCes F57G9.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14700 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14700. Description: sEx14700 [rCes F58A6.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14701 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14701. Description: sEx14701 [rCesF56C9.10a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 03/16/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14702 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14702. Description: sEx14702 [rCes F37B4.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14703 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14703. Description: sEx14703[rCesT27F7.3a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14706 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14706. Description: sEx14706 [rCesF10G7.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/22/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14710 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14710. Description: sEx14710 [rCes F11H8.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14714 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14714. Description: sEx14714 [rCesY37E11B.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 01/20/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14716 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13913. Description: sIs13913 [rCesF01G10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Jin Lin Received: 05/16/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14720 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14720. Description: sEx14720 [rCesF56H11.1b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/08/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14723 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14723. Description: sEx14723[rCesR12E2.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 09/28/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14724 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14724. Description: sEx14724 [rCes W07G1.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14726 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14726. Description: sEx14726 [rCes T13F2.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14727 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14727. Description: sEx14727 [rCesW05H5.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/04/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14730 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14730. Description: sEx14730 [rCes C04F5.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14732 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14732. Description: sEx14732[rCesC36C5.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/04/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14733 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14733. Description: sEx14733[rCesC36C5.10 + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/04/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14734 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14734. Description: sEx14734 [rCesC36C5.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/04/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14736 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14736. Description: sEx14736 [rCes C36C5.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14738 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14738. Description: sEx14738 [rCesF59A7.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/14/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14740 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14740. Description: sEx14740 [rCesR13D11.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 04/03/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14744 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14744. Description: sEx14744 [rCes Y40B10A.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14746 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14746. Description: sEx14746 [rCesY64G10A.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 04/03/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14749 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14749. Description: sEx14749 [rCes C24B9.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14750 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14750. Description: sEx14750 [rCesC50H11.2 + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/04/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14751 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14751. Description: sEx14751[rCesC50H11.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/14/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14755 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14755. Description: sEx14755 [rCes F47D2.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14757 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14757. Description: sEx14757 [rCes H12I19.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14758 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14758. Description: sEx14758 [rCesK05D4.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 04/22/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14760 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14760. Description: sEx14760 [rCes K05D4.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14764 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14764. Description: sEx14764 [rCesT25E12.13::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 04/03/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14765 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14765. Description: sEx14765 [rCes Y6G8.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14767 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14767. Description: sEx14767 [rCes Y9C9A.14::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14768 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14768. Description: sEx14768 [rCes Y9C9A.15::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14770 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14770. Description: sEx14770 [rCes C31A11.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14772 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14772. Description: sEx14772 [rCes R04D3.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14774 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14774. Description: sEx14774 [rCes W07A8.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14775 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14775. Description: sEx14775 [rCesY44A6B.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/24/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14778 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14778. Description: sEx14778 [rCesC25A1.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 05/02/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14781 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14781. Description: sEx14781 [rCes F08G12.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14786 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14786. Description: sEx14786 [rCes R148.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14787 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14787. Description: sEx14787 [rCesF53F4.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14788 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14788. Description: sEx14788 [rCes T04C10.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14789 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14789. Description: sEx14789 [rCes Y38F1A.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14795 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14795. Description: sEx14795 [rCesZC506.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/16/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC148 Species: Caenorhabditis elegans Genotype: dpy-14(e188) let-75(s101) unc-13(e51)/unc-15(e73) I. Description: Heterozygotes are WT and segregate more WT, paralyzed Unc and DpyUncLethals. The DpyUncs are abnormal larvae that die in early larval development (L1). Pick WT to maintain. Previously called myo-1(s101). Mutagen: EMS Outcrossed: x Made by: Rose A Received: 08/01/80 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1480 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-332(s234)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, and dead eggs. Egg lethal. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Rosenbluth R Received: 11/27/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14801 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14801. Description: sEx14801[rCesY61A9LA.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 01/24/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14804 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14804. Description: sEx14804 [rCes T07D4.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14805 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14805. Description: sEx14805 [rCesC02F12.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 06/19/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14807 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14807. Description: sEx14807 [rCes npp-4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 09/13/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1481 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-350(s250) unc-46(e177)/eT1 V. Description: WT strain which segregates WT, Unc-36, dead eggs and DpyUncLet. Lethal late larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Rosenbluth R Received: 11/15/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14810 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14810. Description: sEx14810 [rCes F40G9.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14815 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14815. Description: sEx14815 [rCesT10C6.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/31/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14816 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14816. Description: sEx14816 [rCes F23F12.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14817 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14817. Description: sEx14817 [rCesF28C12.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/08/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14819 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14819. Description: sEx14819 [rCesF35C5.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/20/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14820 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14820. Description: sEx14820 [rCes F58A6.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14822 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14822. Description: sEx14822[rCesK02E11.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/04/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14825 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14825. Description: sEx14825 [rCesW07G1.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/04/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14827 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14827. Description: sEx14827 [rCes Y57A10C.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14829 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14829. Description: sEx14829 [rCes Y57A10C.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1483 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-419(s219)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: R. Rosenbluth Received: 01/19/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14831 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14831. Description: sEx14831 [rCes ZK688.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14832 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14832. Description: sEx14832[rCesC36C5.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/14/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14834 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14834. Description: sEx14834 [rCesR10H1.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 10/15/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14836 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14836. Description: sEx14836 [rCesB0238.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/14/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14838 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14838. Description: sEx14838 [rCesC24B9.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/14/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14839 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14839. Description: sEx14839 [rCesC24B9.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/14/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14840 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14840. Description: sEx14840 [rCesF47D2.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/14/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14842 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14842. Description: sEx14842 [rCes F47D2.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14844 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14844. Description: sEx14844 [rCes F14F8.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14845 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14845. Description: sEx14845 [rCesF14F8.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 04/03/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14847 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14847. Description: sEx14847 [rCesW07A8.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/24/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14848 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14848. Description: sEx14848 [rCesC47B2.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 03/02/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14852 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14852. Description: sEx14852[rCesF15B9.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/23/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14855 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14855. Description: sEx14855 [rCesF20G4.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/16/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14857 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14857. Description: sEx14857 [rCesW07A8.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 01/20/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14858 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14858. Description: sEx14858 [rCesY46G5A.30::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/27/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14860 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14860. Description: sEx14860 [rCes F07B10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14863 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14863. Description: sEx14863 [rCes T05A12.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14865 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14865. Description: sEx14865 [rCesC23H3.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/02/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14873 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14873. Description: sEx14873 [rCes F39G3.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14876 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14876. Description: sEx14876 [rCesF19H8.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 02/22/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14877 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14877. Description: sEx14877 [rCesC05C12.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 01/14/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14878 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14878. Description: sEx14878 [rCesT13A10.11a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 09/28/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14879 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14879. Description: sEx14879 [rCesF54E2.3a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 05/22/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14881 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14881. Description: sEx14881[rCesC06E8.3a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 01/23/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14883 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14883. Description: sEx14883 [rCes Y57G11C.24c::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14885 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14885. Description: sEx14885 [rCesZK54.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14887 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14887. Description: sEx14887 [rCesT17H7.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/21/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14889 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13958. Description: sIs13958 [rCes F28D1.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14892 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs14196. Description: sIs14196 [rCesZK370.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: John Lin Received: 04/10/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14894 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14894. Description: sEx14894 [rCesC36C5.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 11/12/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14895 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14895. Description: sEx14895 [rCesC24B9.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 04/03/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14896 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14896. Description: sEx14896 [rCesW07A8.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 12/10/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14898 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14898. Description: sEx14898 [rCes C01G10.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14899 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14899. Description: sEx14899 [rCes Y108G3AL.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC149 Species: Caenorhabditis elegans Genotype: dpy-14(e188) unc-13(e51) let-83(s97)/unc-15(e73) I. Description: Heterozygotes are WT and segregate more WT, paralyzed Unc and DpyUncLethals. The DpyUnc are abnormal larvae which die in early larval development. Pick WT to maintain. Mutagen: EMS Outcrossed: x Made by: Rose A Received: 06/01/82 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14900 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14900. Description: sEx14900 [rCes ZK632.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14902 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14902. Description: sEx14902 [rCes Y87G2A.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14904 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14904. Description: sEx14904 [rCes C05C10.6a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/23/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14906 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14906. Description: sEx14906 [rCesY53G8AR.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/18/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14907 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14907. Description: sEx14907 [rCes F54D7.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14908 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14908. Description: sEx14908 [rCes Y43F4B.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14909 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14909. Description: sEx14909 [rCes Y119C1B.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14910 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14910. Description: sEx14910 [rCesF09E5.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 03/23/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14912 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14912. Description: sEx14912[rCesY42G9A.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/20/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14915 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14915. Description: sEx14915 [rCes M18.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14917 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14917. Description: sEx14917 [rCes C42C1.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14918 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14918. Description: sEx14918 [rCesF21G4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/18/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14919 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14919. Description: sEx14919 [rCes C27C12.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14921 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14921. Description: sEx14921 [rCes Y48G8AR.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14922 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14922. Description: sEx14922 [rCes F15B10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 08/25/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14923 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14923. Description: sEx14923 [rCes K07H8.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/05/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14924 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14924. Description: sEx14924 [rCes C54D2.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14926 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14926. Description: sEx14926 [rCesK09A11.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 11/06/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14929 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14929. Description: sEx14929 [rCes Y39A1A.19::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14931 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14931. Description: sEx14931 [rCesC34G6.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 02/19/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14933 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14933. Description: sEx14933 [rCes T10H9.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14934 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14934. Description: sEx14934 [rCesZC317.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 09/03/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14935 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14935. Description: sEx14935 [rCes T09E8.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14937 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14937. Description: sEx14937 [rCesF16B4.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 05/23/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14938 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14938. Description: sEx14938 [rCes R13.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14940 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14940. Description: sEx14940 [rCes T14G12.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14944 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14944. Description: sEx14944 [rCes T13H2.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14946 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14946. Description: sEx14946 [rCes C45B11.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14948 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14948. Description: sEx14948 [rCesT13B5.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/02/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14951 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14951. Description: sEx14951 [rCes C04G2.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14952 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14952. Description: sEx14952[rCesF20C5.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/20/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14954 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14954. Description: sEx14954 [rCes F08F3.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14956 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14956. Description: sEx14956 [rCesF42E11.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/30/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14958 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14958. Description: sEx14958 [rCes C27F2.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14959 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14959. Description: sEx14959 [rCes K09A9.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14964 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14964. Description: sEx14964 [rCes C01G8.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14966 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14966. Description: sEx14966 [rCesC27H5.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/16/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14968 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14968. Description: sEx14968 [rCesT01H8.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 02/18/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14969 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14969. Description: sEx14969 [rCesC35B8.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/19/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14974 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14974. Description: sEx14974 [rCes F29D10.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14978 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14978. Description: sEx14978 [rCes T10B11.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14979 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14979. Description: sEx14979 [rCesF57B10.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/16/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14981 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14981. Description: sEx14981[rCesT19D7.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 10/24/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14983 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14983. Description: sEx14983 [rCes Y44E3A.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14985 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14985. Description: sEx14985 [rCesF28B4.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/22/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14986 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14986. Description: sEx14986 [rCes F26A1.14::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14988 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14988. Description: sEx14988 [rCesC37F5.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 08/22/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14990 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14990. Description: sEx14990 [rCes F53B6.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/05/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14993 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14993. Description: sEx14993 contains [rCes ZK1098.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 09/22/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14994 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14994. Description: sEx14994 [rCesR04F11.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/08/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC14995 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx14995. Description: sEx14995 [rCes W02B3.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15000 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15000. Description: sEx15000 [rCes R13F6.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15001 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15001. Description: sEx15001 [rCes F28F5.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15003 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15003. Description: sEx15003[rCesF23F12.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/11/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15005 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15005. Description: sEx15005 [rCesY111B2A.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 04/10/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15006 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15006. Description: sEx15006 [rCes B0238.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15009 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15009. Description: sEx15009 [rCes M28.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/28/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15017 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15017. Description: sEx15017 [rCes Y50E8A.16::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 12/08/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15026 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15026. Description: sEx15026 [rCesF56H11.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/15/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15028 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15028. Description: sEx15028 [rCesC11D2.6a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 01/31/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15032 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15032. Description: sEx15032 [rCesC54A12.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). drn-1::GFP. Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 02/06/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15043 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15043. Description: sEx15043 [rCes T01E8.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 01/10/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15044 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15044. Description: sEx15044 [rCes F01D5.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 01/10/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15046 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15046. Description: sEx15046 [rCesC25D7.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 12/11/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15047 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15047. Description: sEx15047 [rCesC37H5.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/14/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15051 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15051. Description: sEx15051 [rCes C05D9.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/29/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15054 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15054. Description: sEx15054 [rCes ZK697.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15056 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15056. Description: sEx15056 [rCes C56G2.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15057 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15057. Description: sEx15057 [rCes C56G2.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15064 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15064. Description: sEx15064 [rCesC23G10.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/08/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15068 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15068. Description: sEx15068 [rCesF42A10.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 08/16/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15071 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15071. Description: sEx15071 [rCes H43I07.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15073 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15073. Description: sEx15073[rCesY39A1B.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 03/23/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15074 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15074. Description: sEx15074 [rCesF46G10.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 10/24/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15075 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15075. Description: sEx15075 [rCes T27F6.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15076 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15076. Description: sEx15076 [rCes Y54F10AL.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15078 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15078. Description: sEx15078 [rCes K10C8.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/25/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15079 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15079. Description: sEx15079 [rCes D1007.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15082 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15082. Description: sEx15082 [rCes M01G5.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15083 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15083. Description: sEx15083 [rCes H37N21.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15084 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15084. Description: sEx15084 [rCes T28D6.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15103 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15103. Description: sEx15103[rCesF44C8.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/06/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15137 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13046. Description: sIs13046 [rCesC43C3.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: John Lin Received: 11/01/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15138 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13136. Description: sIs13136 [rCesC27D6.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: John Lin Received: 08/28/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15141 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15141. Description: sEx15141[rCesC13D9.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 03/24/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15142 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15142. Description: sEx15142 [rCes C04E6.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15143 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15143. Description: sEx15143 [rCes C04F2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/04/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15144 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15144. Description: sEx15144 [rCes F21F8.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Received: 10/01/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15147 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15147. Description: sEx15147 [rCesF44F4.13::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 12/21/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15148 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15148. Description: sEx15148 [rCesF44F4.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 03/24/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15161 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15161. Description: sEx15161 [rCes F42G2.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15162 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15162. Description: sEx15162 [rCes ZC376.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15165 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15165. Description: sEx15165 [rCesZC204.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/24/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15167 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15167. Description: sEx15167 [rCesY80D3A.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/22/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15170 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15170. Description: sEx15170 [rCes R06F6.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15173 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15173. Description: sEx15173 [rCesC07E3.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/07/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15175 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15175. Description: sEx15175 [rCesY66A7A.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 09/15/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15177 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15177. Description: sEx15177 [rCesC49C3.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 09/15/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15179 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15179. Description: sEx15179 [rCesC02B4.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/06/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15183 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15183. Description: sEx15183 [rCes C05E4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15187 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15187. Description: sEx15187 [rCes F09C6.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15188 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15188. Description: sEx15188 [rCes F45C12.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1519 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf31/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. Original isolation: BO strain BC1261 unc-22(s727) hermaphrodite heat shocked for 1 hour at 34C. Crossed to N2 strain BC1270 unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Picked individual WT hermaphrodites. Screened for the absence of gravid Unc-22. Retained strain as BC1379. Balanced over eT1: BC1379 unc-22(s727)[BO]/nT1[N2] IV; sDf31[BO]/nT1[N2] hermaphrodite crossed to BC 1265 dpy-18(e364)/eT1 III; unc-46(e177)/eT1 V. Pick WT hermaphrodites that twitch in 1% nicotine. Retained one strain that segregated Unc-36 as BC1428. Pseudolinked sDf31 to dpy-18: BC1428 +/eT1 III; sDf31[BO]/eT1 V crossed to BC 1265 dpy-18/eT1 III; unc-46/eT1 V male. Picked WT hermaphrodite F1. From strains segregating Unc-36 but no Dpy or Unc-46 or DpyUnc-46, cross WT hermaphrodites to BC1265 males. From strains producing Dpy, crossed individual WT males to BC70 eT1;eT1. From each cross, crossed WT X WT. Kept one strain producing on Dpy or DpyUncs as S-H716 male. Picked one hermaphrodite to start BC1519. Comments: 1) The LGV region balanced by eT1 should be all BO. 2) Probably the rest of the genome still has a considerable amount from BO since it was crossed to N2 only 4 times. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Tc1? Outcrossed: x Made by: Leo Donati Received: 02/03/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15199 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13915. Description: sIs13915 [rCesH28O16.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-rays Outcrossed: x Made by: Jon Lin Received: 01/20/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15201 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15201. Description: sEx15201[rCesY56A3A.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 02/06/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15208 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15208. Description: sEx15208 [rCesZK757.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1521 Species: Caenorhabditis elegans Genotype: dpy-18(e364) III/eT1 [let-?(s1799)] (III;V); let-327(s247) unc-46(e177) V/eT1 [let-?(s1799)] (III;V). Description: Heterozygotes are WT and throw WT, DpyUncLet and dead eggs. DpyUncLet are translucent, have slow development, are cold sensitive and die as late larvals. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: R. Rosenbluth Received: 07/01/85 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15211 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15211. Description: sEx15211[rCesC50F4.14::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 01/23/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15215 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15215. Description: sEx15215 [rCes Y24D9A.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15216 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15216. Description: sEx15216 [rCes Y51H7C.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15218 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15218. Description: sEx15218 [rCesZK1067.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/08/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15222 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15222. Description: sEx15222[rCesF47B7.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/05/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15224 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15224. Description: sEx15224 [rCes T03D8.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/28/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15226 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15226. Description: sEx15226 [rCes Y69A2AL.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15228 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15228. Description: sEx15228 [rCes R02D3.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15233 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15233. Description: sEx15233[rCesC24G6.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/06/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15234 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15234. Description: sEx15234 [rCesT18H9.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/18/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15236 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15236. Description: sEx15236 [rCesW06B11.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15238 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15238. Description: sEx15238 [rCesC37C3.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 05/25/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15240 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15240. Description: sEx15240 [rCes F14F3.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/10/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15241 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15241. Description: sEx15241 [rCes Y110A7A.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15244 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15244. Description: sEx15244 [rCesF32D1.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/28/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15247 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15247. Description: sEx15247 [rCes F42C5.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15250 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15250. Description: sEx15250 [rCesC50C3.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15251 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15251. Description: sEx15251 [rCes D2007.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15253 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15253. Description: sEx15253 [rCes D2007.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15254 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15254. Description: sEx15254 [rCes K06H7.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15256 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15256. Description: sEx15256 [rCesF44B9.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/08/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15259 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15259. Description: sEx15259 [rCes ZK688.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15260 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15260. Description: sEx15260 [rCes C02C2.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15262 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15262. Description: sEx15262[rCesE01A2.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15263 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15263. Description: sEx15263 [rCes Y40B1B.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15266 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs10341. Description: sIs10341 [rCes T04D1.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15268 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs14115. Description: sIs14115 [rCes C39F7.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15269 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15269. Description: sEx15269 [rCesT03D3.14::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 09/25/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15270 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15270. Description: sEx15270 [rCesR07B5.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/24/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15274 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15274. Description: sEx15274 [rCes ZC487.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15275 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15275. Description: sEx15275 [rCes C56A3.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15277 Species: Caenorhabditis elegans Genotype: dpy-5(e907); sEx15277. Description: sEx15277 rCesZC168.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/15/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15279 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15279. Description: sEx15279 [rCes F40C5.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15280 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15280. Description: sEx15280 [rCes F40C5.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/10/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15281 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15281. Description: sEx15281[rCesT24A6.15::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Received: 11/08/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15282 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15282. Description: sEx15282[rCesT24A6.18::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 03/24/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15286 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15286. Description: sEx15286 [rCesY65B4BR.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/28/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15287 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15287. Description: sEx15287 [rCesC24G6.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/18/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15289 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15289. Description: sEx15289 [rCesT02E9.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/18/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15290 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15290. Description: sEx15290 [rCesC05E4.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 04/03/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15292 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15292. Description: sEx15292 [rCes C25A1.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/21/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15297 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15297. Description: sEx15297 [rCesT24H10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 01/18/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15298 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15298. Description: sEx15298 [rCes C03D6.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15299 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15299. Description: sEx15299 [rCesZK131.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 05/31/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15307 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15307. Description: sEx15307 [rCes Y43F4B.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15308 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15308. Description: sEx15308 [rCesM03C11.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/17/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15310 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15310. Description: sEx15310 [rCes R04A9.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15315 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15315. Description: sEx15315 [rCes K10G9.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15317 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15317. Description: sEx15317 [rCesZK896.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 02/20/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15319 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15319. Description: sEx15319 [rCesC54D10.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/06/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15328 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15328. Description: sEx15328 [rCesF35B12.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 02/17/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15331 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15331. Description: sEx15331[rCesC40D2.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 05/22/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15334 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15334. Description: sEx15334 [rCes K04G11.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15338 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13769. Description: sIs13769 [rCesF54F2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: John Lin Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15342 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15342. Description: sEx15342 [rCes W02B12.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15345 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15345. Description: sEx15345 [rCesC03A7.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/14/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15347 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15347. Description: sEx15347 [rCes F53B1.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15352 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15352. Description: sEx15352 [rCes F54C9.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15354 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15354. Description: sEx15354 [rCes JC8.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15355 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15355. Description: sEx15355 [rCes ZK856.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15356 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15356. Description: sEx15356 [rCesY110A2AL.13::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/16/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15357 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15357. Description: sEx15357 [rCes F52A8.6b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15358 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15358. Description: sEx15358 [rCesC42C1.14::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/22/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15363 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15363. Description: sEx15363 [rCes C01G10.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15369 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15369. Description: sEx15369 [rCesK01D12.12::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/06/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15374 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15374. Description: sEx15374 [rCes C04B4.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15376 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15376. Description: sEx15376 [rCes C38C10.5a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15377 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15377. Description: sEx15377 [rCes Y48C3A.14::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15381 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15381. Description: sEx15381 [rCes C32E12.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15383 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15383. Description: sEx15383[rCesF15A2.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/30/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15384 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15384. Description: sEx15384 [rCesD2045.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 09/18/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15390 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15390. Description: sEx15390 [rCes M142.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15398 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15398. Description: sEx15398 [rCesF56D1.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 05/21/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15400 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15400. Description: sEx15400 [rCesF53A9.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 11/30/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15406 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15406. Description: sEx15406 [rCesF53H8.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 06/20/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15410 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11495. Description: sIs11495 [rCes F33H2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15411 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs11803. Description: sIs11803 [rCesEEED8.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: John Lin Received: 07/10/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15412 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs14214. Description: sIs14214 [rCesF10E9.6a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: John Lin Received: 01/11/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15418 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15418. Description: sEx15418 [rCes AC7.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15419 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15419. Description: sEx15419 [rCes T09F3.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15422 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15422. Description: sEx15422 [rCes C37H5.6b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15424 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15424. Description: sEx15424 [rCes Y73F8A.34::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 02/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15426 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15426. Description: sEx15426 [rCes ZK177.8b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15428 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15428. Description: sEx15428 [rCes F43E2.7a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15430 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15430. Description: sEx15430 [rCes ZK973.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15431 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15431. Description: sEx15431[rCesF52D10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 09/19/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15433 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15433. Description: sEx15433 [rCes R11D1.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15434 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15434. Description: sEx15434 [rCes R11D1.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15435 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15435. Description: sEx15435 [rCesF28A10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 05/21/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15438 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15438. Description: sEx15438 [rCesK08A8.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/18/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15440 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15440. Description: sEx15440 [rCesC50B6.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 12/01/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15442 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15442. Description: sEx15442 [rCes Y55D9A.2b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15444 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15444. Description: sEx15444 [rCes Y53G8AL.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15445 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15445. Description: sEx15445 [rCes W10D5.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15451 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15451. Description: sEx15451 [rCes D1007.12::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. GFP+ animals have very few progeny, remove dumpy animals to keep them from taking over the plate. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15452 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15452. Description: sEx15452 [rCes F07D10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/29/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15460 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15460. Description: sEx15460 [rCes R11F4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 03/02/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15461 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15461. Description: sEx15461[rCesR12E2.13::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 09/06/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15463 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15463. Description: sEx15463[rCesR151.2b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 08/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15464 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15464. Description: sEx15464 [rCesT06D8.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 04/16/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15466 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15466. Description: sEx15466 [rCes T12D8.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15469 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15469. Description: sEx15469 [rCesC28C12.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15476 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15476. Description: sEx15476 [rCes T22H9.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15479 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15479. Description: sEx15479 [rCes F22F7.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1548 Species: Caenorhabditis elegans Genotype: unc-22(s7) let-67(s214)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul and Twitcher Steriles. The sterility can be rescued by male sperm. Mutagen: EMS Outcrossed: x Made by: Moerman & Rogalski Received: 12/30/94 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15484 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15484. Description: sEx15484 [rCes C29G2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15486 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15486. Description: sEx15486 [rCes T10B5.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15488 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15488. Description: sEx15488 [rCes Y45G12C.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15489 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15489. Description: sEx15489 [rCes F13B12.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1549 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-349(s217)/eT1 V. Description: WT strain which segregates WT, Unc-36, dead eggs and DpyUncLets. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: R. Rosenbluth Received: 01/19/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15491 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15491. Description: sEx15491[rCesC06G3.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 04/03/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15496 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15496. Description: sEx15496 [rCesC34F11.3a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/03/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15498 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15498. Description: sEx15498 [rCesC34F11.3b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/03/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15500 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15500. Description: sEx15500 [rCes C18F10.7a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15504 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15504. Description: sEx15504 [rCesB0286.4c::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/13/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15507 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15507. Description: sEx15507 [rCesT08B2.7b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/23/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15508 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15508. Description: sEx15508 [rCes K09A9.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15510 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15510. Description: sEx15510 [rCes H06H21.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15512 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15512. Description: sEx15512 [rCes F36D4.5b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15513 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15513. Description: sEx15513 [rCes Y34D9A.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15514 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15514. Description: sEx15514 contains [rCes F55A4.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 10/26/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15515 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15515. Description: sEx15515 [rCesT13A10.11b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 09/28/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15517 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15517. Description: sEx15517 [rCes W09G3.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 02/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15519 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13978. Description: sIs13978 [rCesF59B8.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: John Lin Received: 11/10/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15521 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13743. Description: sIs13743 [rCesT19B10.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: John Lin Received: 01/24/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15527 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15527. Description: sEx15527 [rCes K12C11.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/09/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15528 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15528. Description: sEx15528 [rCesY48G9A.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/13/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15529 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15529. Description: sEx15529 [rCes Y71F9AL.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15530 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15530. Description: sEx15530 [rCes Y76B12C.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15531 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15531. Description: sEx15531 [rCes ZK1058.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15536 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15536. Description: sEx15536 [rCes T19B10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1554 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf32 unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: EMS Outcrossed: x Made by: R. Rosenbluth Received: 07/01/85 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15540 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15540. Description: sEx15540 [rCes F47G9.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Domena Tu Received: 12/19/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15545 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15545. Description: sEx15545 [rCes F53E10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15553 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15553. Description: sEx15553 [rCes Y73C8B.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15556 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15556. Description: sEx15556 [rCes C07G3.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15557 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15557. Description: sEx15557 [rCes F47C10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15565 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15565. Description: sEx15565 [rCesH23N18.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/26/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15567 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15567. Description: sEx15567 [rCesF26G5.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15568 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15568. Description: sEx15568 [rCes ZC317.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15570 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15570. Description: sEx15570 contains [rCes K12B6.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Lily Fang Received: 10/08/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15571 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15571. Description: sEx15571 [rCes K11G9.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15574 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15574. Description: sEx15574 [rCesM03E7.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/23/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15578 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15578. Description: sEx15578 [rCesC55H1.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 06/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15579 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13363. Description: sIs13363 [rCes D2024.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15588 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15588. Description: sEx15588 [rCes C17H12.13::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15590 Species: Caenorhabditis elegans Genotype: dpy-5(e907); sEx15590. Description: sEx15590 [rCesC25E10.12::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/15/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15592 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15592. Description: sEx15592[rCesC27C12.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Domena Tu Received: 01/24/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15594 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15594. Description: sEx15594 [rCesC49G7.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/23/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15595 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15595. Description: sEx15595 [rCes F08C6.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15598 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15598. Description: sEx15598 [rCesF10C1.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/05/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15603 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15603. Description: sEx15603[rCesF52H3.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 11/30/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15605 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15605. Description: sEx15605 [rCes F56A3.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15606 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15606. Description: sEx15606 [rCesF58B3.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 11/06/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15608 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15608. Description: sEx15608 [rCesK06A1.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 11/06/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15614 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15614. Description: sEx15614 [rCes R02E12.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/30/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15615 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15615. Description: sEx15615 [rCes R10F2.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 08/25/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15619 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15619. Description: sEx15619 [rCes T01D3.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 12/01/11 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15622 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15622. Description: sEx15622[rCesT07G12.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 10/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15631 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15631. Description: sEx15631 [rCes Y39A3CR.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15634 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15634. Description: sEx15634 [rCesE01F3.1b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15639 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15639. Description: sEx15639 [rCesC38H2.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: 0 Outcrossed: x06 Made by: Lily Fang Received: 06/07/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15640 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15640. Description: sEx15640 [rCes B0284.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15643 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15643. Description: sEx15643 [rCes C16C10.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15645 Species: Caenorhabditis elegans Genotype: dpy-5(e907); sEx15645. Description: sEx15645 [rCesB0285.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/15/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15648 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15648. Description: sEx15648 [rCes F25B5.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15649 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15649. Description: sEx15649 [rCesF28B12.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/09/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15650 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15650. Description: sEx15650 [rCesF44E7.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/23/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15652 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15652. Description: sEx15652 [rCes C05C8.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15653 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15653. Description: sEx15653[rCesC03G6.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15659 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15659. Description: sEx15659 [rCes EGAP798.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15667 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15667. Description: sEx15667 [rCes C29F9.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15671 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15671. Description: sEx15671[rCesT17H7.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 01/03/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15680 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15680. Description: sEx15680 [rCesC47A10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 03/31/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15682 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15682. Description: sEx15682 [rCes R06F6.5a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15683 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs14317. Description: sIs14317 [rCesC46C11.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: John Lin Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15684 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs14288. Description: sIs14288 [rCes F09G2.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15685 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15685. Description: sEx15685 [rCes D2096.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15692 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15692. Description: sEx15692 [rCes W04B5.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15693 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15693. Description: sEx15693[rCesT03F7.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15694 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15694. Description: sEx15694 [rCesY48A6B.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 09/19/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15698 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15698. Description: sEx15698 [rCes K02G10.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC157 Species: Caenorhabditis elegans Genotype: dpy-14(e188) unc-13(e51) let-80(s96)/unc-15(e73) I. Description: Heterozygotes are WT and segregate more WT, paralyzed Unc and Lethal DpyUncs. Lethal early larval. Pick WT to maintain. Mutagen: EMS Outcrossed: x Made by: Rose A Received: 06/01/82 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15700 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15700. Description: sEx15700 [rCesT23G11.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 01/03/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15703 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15703. Description: sEx15703[rCesW02H5.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 02/02/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15705 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15705. Description: sEx15705 [rCesC27B7.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 04/23/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15707 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15707. Description: sEx15707 [rCesC50F4.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 07/06/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15709 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15709. Description: sEx15709 [rCesZK337.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Lily Fang Received: 10/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15712 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15712. Description: sEx15712 [rCes Y50D7A.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15715 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15715. Description: sEx15715 [rCes Y54F10AL.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15716 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15724. Description: sEx15724 [rCesD1014.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/19/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1572 Species: Caenorhabditis elegans Genotype: +/eT1 III; let-401(s193) dpy-11(e224) unc-42(e270)/eT1 V. Description: WT strain which segregates WT, Unc-36, dead eggs and DpyUncLets. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Roglaski/Rosenbluth Received: 10/01/85 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15720 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15720. Description: sEx15720 [rCes ZK829.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15723 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15723. Description: sEx15723 [rCes C37C3.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15724 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15724. Description: sEx15724 [rCesD1014.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/07/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15728 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15728. Description: sEx15728 [rCesF41E6.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 04/24/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15729 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15729. Description: sEx15729 [rCes Y45F3A.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1573 Species: Caenorhabditis elegans Genotype: +/eT1 III; let-402(s127) dpy-11(e224) unc-42(e270)/eT1 V. Description: WT strain which segregates WT, Unc-36, dead eggs and DpyUncLets. Lethal mid larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: R. Rosenbluth Received: 10/01/85 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15733 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs14205. Description: sIs14205 [rCesD1046.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-rays Outcrossed: x0 Made by: John Lin Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15734 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs14164. Description: sIs14164 [rCesC37C3.6b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: X-rays Outcrossed: x0 Made by: John Lin Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15735 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15735. Description: sEx15735 [rCesB0464.5a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1574 Species: Caenorhabditis elegans Genotype: +/eT1 III; let-403(s120) dpy-11(e224) unc-42(e270)/eT1 V. Description: WT strain which segregates WT, Unc-36, dead eggs and DpyUncLets. Lethal mid-late larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Rogalski/Rosenbluth Received: 10/01/85 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15741 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15741. Description: sEx15741[rCesT15H9.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Domena Tu Received: 02/29/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15745 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15745. Description: sEx15745 [rCesC37C3.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15746 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15746. Description: sEx15746 [rCesY62H9A.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15747 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15747. Description: sEx15747 [rCesF02E8.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15749 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15749. Description: sEx15749 [rCesF46C5.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15752 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15752. Description: sEx15752[rCesY54G11A.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15754 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15754. Description: sEx15754 [rCesC52E12.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15755 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15755. Description: sEx15755 [rCesC32F10.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15757 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15757. Description: sEx15757 [rCesT28C6.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15760 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15760. Description: sEx15760 [rCesF11G11.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15761 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15761. Description: sEx15761[rCesF55B11.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15766 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15766. Description: sEx15766 [rCesM142.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15767 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15767. Description: sEx15767 [rCesZK550.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15771 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15771. Description: sEx15771[rCesC30F12.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15773 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15773. Description: sEx15773[rCesF23F1.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15774 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15774. Description: sEx15774 [rCesK06A4.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15776 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15776. Description: sEx15776 [rCes Y47A7.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15777 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15777. Description: sEx15777 [rCesF10D2.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/10/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15783 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15783. Description: sEx15783[rCesY54G2A.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15784 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15784. Description: sEx15784 [rCesK02D7.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15787 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15787. Description: sEx15787 [rCesF20G4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15789 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15789. Description: sEx15789 [rCesZC142.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15791 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15791. Description: sEx15791[rCesF48G7.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15793 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15793. Description: sEx15793[rCesR03H4.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15795 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15795. Description: sEx15795 [rCesF47D2.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15797 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15797. Description: sEx15797 [rCesF47D2.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15798 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15798. Description: sEx15798 [rCesF40H7.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15800 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15800. Description: sEx15800 [rCesF40H7.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15802 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15802. Description: sEx15802[rCesF40H7.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15804 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15804. Description: sEx15804 [rCesF40H7.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15806 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15806. Description: sEx15806 [rCesT24E12.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15808 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15808. Description: sEx15808 [rCesF56H9.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15809 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15809. Description: sEx15809 [rCesW05B10.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15828 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15828. Description: sEx15828 [rCesF20D1.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15831 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15831. Description: sEx15831 [rCesF09B9.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/14/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15833 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15833. Description: sEx15833 [rCes F32B6.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15835 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15835. Description: sEx15835 [rCesK11C4.3a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 01/23/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15837 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15837. Description: sEx15837 [rCesC05C8.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/01/05 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15839 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs14542. Description: sIs14542[rCesT01D3.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: John Lin Received: 05/29/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15840 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13611. Description: sIs13611 [rCes M04C9.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15841 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sIs13484. Description: sIs13484 [rCesF46H5.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: John Lin Received: 06/19/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15845 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15845. Description: sEx15845 [rCes K05D4.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15846 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15846. Description: sEx15846 [rCes M176.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15860 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15860. Description: sEx15860 [rCesR04D3.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/04/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15863 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15863. Description: sEx15863[rCesY57A10C.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15866 Species: Caenorhabditis elegans Genotype: dpy-5(e907); sEx15866. Description: sEx15866 [rCesC24B9.12::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/15/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15868 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15868. Description: sEx15868 [rCesY47G7B.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 04/03/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15869 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15869. Description: sEx15869 [rCes K08G2.12::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15870 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15870. Description: sEx15870 [rCesF11A5.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 12/10/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15871 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15871. Description: sEx15871[rCesF11A5.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15876 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15876. Description: sEx15876 [rCes F25E5.13::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15882 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15882. Description: sEx15882 [rCes Y39A3CR.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15888 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15888. Description: sEx15888 [rCes F46C5.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15889 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15889. Description: sEx15889 [rCes Y57G7A.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/08/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15890 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15890. Description: sEx15890 [rCes Y54G11A.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15893 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15893. Description: sEx15893 [rCesF20G4.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Allan Mah Received: 01/24/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15894 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15894. Description: sEx15894 [rCes T28C6.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC159 Species: Caenorhabditis elegans Genotype: dpy-5(e61) unc-13(e51) I; sDp1 (I;f). Description: Animals with the duplication are WT. Animals which have lost the duplication are DpyUnc. Segregates 2% males. The males are sterile. The duplication is homozygous lethal. Mutagen: Gamma ray Outcrossed: x0 Made by: Rose A Received: 07/01/80 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15905 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15905. Description: sEx15905 [rCes F55A11.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15914 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15914. Description: sEx15914 [rCes K06A4.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15916 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15916. Description: sEx15916 [rCes Y54G11A.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15918 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15918. Description: sEx15918 [rCes H27A22.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15924 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15924. Description: sEx15924 [rCesF59B10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 03/17/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15925 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15925. Description: sEx15925 [rCesM02D8.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Allan Mah Received: 04/10/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15929 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15929. Description: sEx15929 [rCes F42G2.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15931 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15931. Description: sEx15931[rCesF52A8.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/22/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15932 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15932. Description: sEx15932[rCesF53F10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15937 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15937. Description: sEx15937 [rCes C50C10.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15938 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15938. Description: sEx15938 [rCes F15E6.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15940 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15940. Description: sEx15940 [rCesR11G11.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 04/03/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15957 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15957. Description: sEx15957 [rCes R04D3.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15959 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15959. Description: sEx15959 [rCes C50B6.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15966 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15966. Description: sEx15966 [rCesH27D07.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 09/29/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15973 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15973. Description: sEx15973 [rCes Y75B8A.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15987 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15987. Description: sEx15987 [rCes C13D9.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15994 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15994. Description: sEx15994 [rCes R13D7.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC15999 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx15999. Description: sEx15999 [rCesC51E3.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC160 Species: Caenorhabditis elegans Genotype: dpy-14(e188) unc-13(e51) let-87(s106)/unc-15(e73) I. Description: Heterozygotes are WT and segregate WT, paralyzed Unc and DpyUncLethal. Lethal early larval (L1). Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Rose A Received: 08/01/80 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16004 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16004. Description: sEx16004 [rCes F56D5.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16010 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16010. Description: sEx16010 [rCes F54B8.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16016 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16016. Description: sEx16016 [rCesF19B2.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 01/09/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16019 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16019. Description: sEx16019 [rCes K03D3.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16021 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16021. Description: sEx16021 [rCes F10A3.15::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16028 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16028. Description: sEx16028 [rCes Y9C9A.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16030 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16030. Description: sEx16030 [rCesY9C9A.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 04/03/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16049 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16049. Description: sEx16049 [rCes C46E10.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16051 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16051. Description: sEx16051 [rCes F07F6.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16057 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16057. Description: sEx16057 [rCesF09C12.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 09/28/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16064 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16064. Description: sEx16064 [rCesC50B6.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 07/24/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16070 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16070. Description: sEx16070 [rCes C41G6.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16071 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16071. Description: sEx16071 [rCes C41G6.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16072 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16072. Description: sEx16072 [rCes ZK697.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16074 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16074. Description: sEx16074 [rCes B0454.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16083 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16083. Description: sEx16083[rCesZK652.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 01/31/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16086 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16086. Description: sEx16086 [rCes F08F8.3a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16092 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16092. Description: sEx16092[rCesK07E12.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 01/20/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16094 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16094. Description: sEx16094 [rCes F52C9.8a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16095 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16095. Description: sEx16095 [rCesF25F2.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16096 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16096. Description: sEx16096 [rCes F37C12.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16099 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16099. Description: sEx16099 [rCes T08A11.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16107 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16107. Description: sEx16107 [rCes C44C3.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16112 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16112. Description: sEx16112[rCesY55F3AM.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 04/03/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16116 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16116. Description: sEx16116 [rCes F49H6.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16118 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16118. Description: sEx16118 [rCes F10A3.13::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16124 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16124. Description: sEx16124 [rCesF35G12.4a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 01/04/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16125 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16125. Description: sEx16125 [rCes F54D8.3a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16132 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16132. Description: sEx16132[rCesZK688.6a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 08/30/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16140 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16140. Description: sEx16140 [rCesC51E3.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16142 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16142. Description: sEx16142 [rCes B0454.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16151 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16151. Description: sEx16151[rCesF02E8.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Allan Mah Received: 01/24/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16156 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16156. Description: sEx16156 [rCesC05D11.7b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 05/23/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16158 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16158. Description: sEx16158 [rCesT04C9.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 01/03/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16159 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16159. Description: sEx16159 [rCes B0393.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16163 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16163. Description: sEx16163 [rCes T04A8.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16164 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16164. Description: sEx16164 [rCesF09F7.7a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 11/30/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16165 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16165. Description: sEx16165 [rCesR09E12.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 03/03/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16169 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16169. Description: sEx16169 [rCesF26F4.7::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/28/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16177 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16177. Description: sEx16177 [rCes ZC404.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16188 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16188. Description: sEx16188 [rCes C44C3.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16192 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16192. Description: sEx16192[rCesT04C9.1b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Allan Mah Received: 06/19/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16196 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16196. Description: sEx16196 [rCesC16A3.10b::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 02/20/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC162 Species: Caenorhabditis elegans Genotype: dpy-14(e188) unc-13(e51) let-88(s132)/unc-15(e73) I. Description: Heterozygotes are WT and segregate WT, paralyzed Unc and DpyUncLethal. The DpyUncs are abnormal larvae which die in early larval development. Pick WT to maintain. Mutagen: EMS Outcrossed: x Made by: Rose A Received: 06/01/82 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16201 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16201. Description: sEx16201 [rCes F31E3.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16202 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16202. Description: sEx16202 [rCes C17F4.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16203 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16203. Description: sEx16203 [rCes F07B10.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16205 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16205. Description: sEx16205 [rCes Y9C9A.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16236 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16236. Description: sEx16236 [rCesT17E9.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 07/31/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16238 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16238. Description: sEx16238 [rCesT04C9.1a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Allan Mah Received: 06/19/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16261 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16261. Description: sEx16261 [rCes F28F5.3a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16278 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16278. Description: sEx16278 [rCesF57B9.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/29/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16286 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16286. Description: sEx16286 [rCes C09E7.8a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16288 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16288. Description: sEx16288 [rCes C56G2.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16292 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16292. Description: sEx16292[rCesBE0003N10.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x Made by: Domena Tu Received: 06/19/08 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16293 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16293. Description: sEx16293[rCesF58B6.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 05/31/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16305 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16305. Description: sEx16305 [rCes C05D2.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16311 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16311. Description: sEx16311 [rCes K01A11.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16312 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16312. Description: sEx16312 [rCes C36E8.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16314 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16314. Description: sEx16314 [rCes F44D12.2::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16317 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16317. Description: sEx16317 [rCes F13H10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16322 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16322. Description: sEx16322 [rCes F58A4.10::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16323 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16323. Description: sEx16323 [rCes R53.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16326 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16326. Description: sEx16326 [rCes T03D8.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16329 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16329. Description: sEx16329 [rCesF20B10.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 10/01/06 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16336 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16336. Description: sEx16336 [rCesY116A8C.35::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 02/20/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16338 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16338. Description: sEx16338 [rCes F59G1.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16339 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16339. Description: sEx16339 [rCes F13H6.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16340 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16340. Description: sEx16340 [rCes Y106G6H.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16344 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16344. Description: sEx16344 [rCes B0261.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16347 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16347. Description: sEx16347 [rCes B0513.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16354 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16354. Description: sEx16354 [rCes ZC434.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16358 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16358. Description: sEx16358 [rCes C15H11.9::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16365 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16365. Description: sEx16365 [rCes F26F4.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16367 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16367. Description: sEx16367 [rCes F39H11.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16377 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16377. Description: sEx16377 [rCes C47D12.6::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16380 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16380. Description: sEx16380 [rCes D2021.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16384 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16384. Description: sEx16384 [rCes F35B3.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC16434 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx16434. Description: sEx16434 [rCes K11H3.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 12/12/12 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC165 Species: Caenorhabditis elegans Genotype: dpy-14(e188) unc-13(e51) let-89(s133)/unc-15(e73) I. Description: Heterozygotes are WT and segregate WT, paralyzed Unc and DpyUncLethal. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Ann Rose Received: 09/01/82 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC17009 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx17009. Description: sEx17009 [rCesT13F2.11::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 03/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC17132 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx17132. Description: sEx17132 [rCesC39E6.4::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 06/14/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC17322 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx17322. Description: sEx17322 contains [rCesZC373.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: David Lee Received: 11/24/09 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC17441 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx17441. Description: sEx17441 [rCes F58E10.3::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Baillie Lab Received: 05/07/13 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1751 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-466(s1063) unc-46(e177)/eT1 V. Description: WT strain which segregates WT, Unc-36, dead eggs and DpyUncs. The DpyUncs throw DpyUncs that are Sterile adults. (Mel->Sterile adult in F2.) Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 11/15/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC177 Species: Caenorhabditis elegans Genotype: unc-22(s17) IV. Description: Twitcher. Both heterozygotes and homozygotes twitch in 1% nicotine. Mutagen: EMS Outcrossed: x Made by: D. Moerman Received: 08/01/80 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1772 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-334(s908)/eT1 V. Description: Heterozygotes are WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: M. Khosla Received: 02/03/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1781 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf33 unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Gamma Rays Outcrossed: x Made by: R. Devlin Received: 04/28/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1782 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf34 unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Gamma Rays Outcrossed: x Made by: R. Devlin Received: 04/15/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1784 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) sDf36/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: No mutagen used Outcrossed: x Made by: R. Rosenbluth Received: 07/01/85 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1785 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) sDf30/eT1 V. Description: WT strain that segregates WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Gamma Rays Outcrossed: x Made by: R. Rosenbluth Received: 01/19/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC17993 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx17993. Description: sEx17993 contains [rCesC17E4.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu / Allan Ma Received: 03/03/10 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC18 Species: Caenorhabditis elegans Genotype: unc-22(s13) IV. Description: Twitcher Unc. Heterozygotes twitch in 1% Nicotine. Recessive. Mutagen: EMS Outcrossed: x Made by: Moerman D Received: 08/01/80 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC184 Species: Caenorhabditis elegans Genotype: dpy-14(e188) unc-13(e51) bli-4(s90)/unc-15(e73) I. Description: Heterozygotes are WT and segregate WT, paralyzed Unc and DpyUncLethals. The DpyUncs are abnormal larvae which die in late larval development. Pick WT to maintain. s90 previously called let-77. See also WBPaper00003507. CGC received new stock 3/01. Mutagen: EMS Outcrossed: x Made by: Ann Rose Received: 09/01/82 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC1907 Species: Caenorhabditis elegans Genotype: let-68(s1081) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul and LetUncTwitcher. Lethal ??. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Denise Clark Received: 09/02/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1912 Species: Caenorhabditis elegans Genotype: let-652(s1086) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Egl, dead eggs and UncLets. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Denise Clark Received: 01/05/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1919 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-343(s1025)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Egg lethal. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: R. Johnsen Received: 04/15/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1922 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-342(s1029) unc-46(e177)/eT1 V. Description: WT strain that segregates WT, Unc-36, dead eggs and DpyUncLets. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: R. Johnsen Received: 02/03/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1924 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-340(s1022)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: R. Johnsen Received: 02/03/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1925 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sos-1(s1031) unc-46(e177)/eT1 V. Description: WT strain that segregates WT, Unc-36 (eT1 homozygotes), and dead eggs. s1031 is an egg lethal. Maintain by picking WT. Previously called let-341(s1031). Mutagen: EMS Outcrossed: x Made by: R. Johnsen Received: 01/19/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1927 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-347(s1035) unc-46(e177)/eT1 V. Description: WT strain that segregates WT, Unc-36, dead eggs and DpyUncLets. Lethal late larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: R. Johnsen Received: 02/03/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1958 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncs and dead eggs. Maintain by picking WT. Mutagen: Outcrossed: x Made by: Rosenbluth R Received: 11/05/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC196 Species: Caenorhabditis elegans Genotype: dpy-5(e61) dpy-14(e188) rec-1(s180) I. Description: Dpy. Best maintained at 16C (dpy-14 is ts). High recombination. Mutagen: Outcrossed: x Made by: Ann Rose Received: 01/28/88 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC1972 Species: Caenorhabditis briggsae Genotype: Cbr-unc(s1270). Description: C. briggsae strain. Twitcher. Hets twitch only in prescence of nicotine. Mutagen: EMS Outcrossed: x2 Made by: S. Bird Received: 05/07/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1977 Species: Caenorhabditis briggsae Genotype: Cbr-unc(s1275). Description: C. briggsae strain. Unc-tends to coil. Mutagen: EMS Outcrossed: x1 Made by: S. Bird Received: 05/07/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1983 Species: Caenorhabditis briggsae Genotype: Cbr-dpy(s1281). Description: C. briggsae strain. Chubby. Moves poorly. Mutagen: EMS Outcrossed: x1 Made by: S. Bird Received: 05/07/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC199 Species: Caenorhabditis elegans Genotype: sDf6/unc-15(e73) I. Description: Heterozygotes are WT and segregate WT, Paralyzed Unc and Lethals (L1). Pick WT to maintain. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Formaldehyde Outcrossed: x Made by: Rose A Received: 12/01/81 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC1999 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; nDf18/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. Mutagen: Outcrossed: x Made by: R. Rosenbluth Received: 11/30/87 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC200 Species: Caenorhabditis elegans Genotype: unc-22(s12) IV. Description: Twitcher Unc. Recessive. Heterozygotes twitch in 1% Nicotine. Mutagen: EMS Outcrossed: x Made by: Moerman D Received: 07/01/80 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2001 Species: Caenorhabditis elegans Genotype: let-98(s1117) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, UncLet and dead eggs. Lethal late larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: D. Clark Received: 12/12/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2003 Species: Caenorhabditis elegans Genotype: let-96(s1112) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, Lethal Twitchers and dead eggs. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: D. Clark Received: 12/12/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC20063 Species: Caenorhabditis elegans Genotype: dpy-5(e907) I; sEx20063. Description: sEx20063[rCesF44E5.5::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525). Mutagen: Outcrossed: x0 Made by: Domena Tu Received: 03/27/07 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2008 Species: Caenorhabditis elegans Genotype: unc-22(s7) unc-31(e169) let-315(s1101)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, UncLet and dead eggs. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Clark D Received: 08/27/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2010 Species: Caenorhabditis elegans Genotype: let-60(s1124) unc-22(s7) unc-31(e169) IV/nT1 (IV;V). Description: Heterozygotes are WT and segregate WT, Vul and UncLethals. Strong allele of let-60. Unc Twitchers arrest as rod-like early larvae; rare escapers die as Vul adults. Mutagen: EMS Outcrossed: x Made by: Denise Clark Received: 12/03/97 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2013 Species: Caenorhabditis elegans Genotype: unc-22(s7) let-97(s1121) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, UncLet (Twitcher), Vul, and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: D. Clark Received: 12/12/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2020 Species: Caenorhabditis elegans Genotype: let-70(s1132) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, UncLet (twitchers) and dead eggs. Lethal early larval. Maintain by picking WT. See also WBPaper00002501. Mutagen: EMS Outcrossed: x Made by: Clark D Received: 01/14/92 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2021 Species: Caenorhabditis elegans Genotype: let-289(s1133) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vuls, early larval lethals which are Unc and Twitch, and dead eggs. Maintain by picking WT. Not outcrossed! Mutagen: EMS Outcrossed: x0 Made by: Denise Clark Received: 03/01/96 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: BC2024 Species: Caenorhabditis elegans Genotype: let-291(s1139) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vuls, early larval lethals which are Unc and Twitch, and dead eggs. Maintain by picking WT. Not outcrossed! Mutagen: EMS Outcrossed: x0 Made by: Denise Clark Received: 03/01/96 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: BC2029 Species: Caenorhabditis elegans Genotype: let-60(s1155) unc-22(s7) unc-31(e169) IV/nT1 (IV;V). Description: Heterozygotes are WT and segregate WT, Vul and Twitcher UncLethals. s1155 is viable; 14% Vul. Mutagen: EMS Outcrossed: x Made by: Denise Clark Received: 12/03/97 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2034 Species: Caenorhabditis elegans Genotype: let-292(s1146) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vuls, early larval lethals which are Unc and Twitch, and dead eggs. Maintain by picking WT. Not outcrossed! Mutagen: EMS Outcrossed: x0 Made by: Denise Clark Received: 03/01/96 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: BC2035 Species: Caenorhabditis elegans Genotype: unc-22(s7) unc-31(e169) let-317(s1153)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Egl, UncLet and dead eggs. Lethal at embryo-early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Clark D Received: 08/27/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2036 Species: Caenorhabditis elegans Genotype: let-100(s1160) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, UncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: D. Clark Received: 12/12/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2037 Species: Caenorhabditis elegans Genotype: let-293(s1166) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are short and somewhat Egl. Offspring are short. Segregate Vuls, early larval lethals which are Unc and Twitch, and dead eggs. Not outcrossed! Mutagen: EMS Outcrossed: x0 Made by: Denise Clark Received: 03/01/96 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: BC2047 Species: Caenorhabditis elegans Genotype: let-290(s1140) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vuls, early larval lethals which are Unc and Twitch, and dead eggs. Maintain by picking WT. Not outcrossed! Mutagen: EMS Outcrossed: x0 Made by: Denise Clark Received: 03/01/96 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: BC2049 Species: Caenorhabditis elegans Genotype: let-658(s1149) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, UncLet, Egl and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: D. Clark Received: 03/04/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2053 Species: Caenorhabditis elegans Genotype: let-307(s1171) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Egl, Lethal Twitchers and dead eggs. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: D. Clark Received: 12/12/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2060 Species: Caenorhabditis elegans Genotype: let-311(s1195) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, UncLet and dead eggs. Lethal late larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: D. Clark Received: 12/12/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2061 Species: Caenorhabditis elegans Genotype: let-69(s1111) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Egl, Lethal Twitchers and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Clark D Received: 01/14/92 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2063 Species: Caenorhabditis elegans Genotype: unc-22(s7) let-309(s1115) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, UncLet and dead eggs. Lethal late larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: D. Clark Received: 12/12/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2067 Species: Caenorhabditis elegans Genotype: let-659(s1152) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, UncLet, Egl and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: D. Clark Received: 03/04/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2069 Species: Caenorhabditis elegans Genotype: let-651(s1165) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, Lethals and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Clark D Received: 01/14/92 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2070 Species: Caenorhabditis elegans Genotype: let-294(s1103) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vuls, early larval lethals which are Unc and Twitch, and dead eggs. Maintain by picking WT. Not outcrossed! Mutagen: EMS Outcrossed: x0 Made by: Denise Clark Received: 03/01/96 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: BC2079 Species: Caenorhabditis elegans Genotype: unc-22(s7) unc-31(e169) let-301(s1134)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, UncLet, and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Clark D Received: 08/30/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2088 Species: Caenorhabditis elegans Genotype: let-295(s1175) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vuls, early larval lethals which are Unc and Twitch, and dead eggs. Maintain by picking WT. Not outcrossed! Mutagen: EMS Outcrossed: x0 Made by: Denise Clark Received: 03/01/96 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: BC2094 Species: Caenorhabditis elegans Genotype: unc-22(s7) unc-31(e169) let-313(s1135)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, Sterile Unc, and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Clark D Received: 10/14/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC21 Species: Caenorhabditis elegans Genotype: unc-22(s8) IV. Description: Twitcher Unc. Recessive. Heterozygotes twitch in 1% Nicotine. Mutagen: EMS Outcrossed: x Made by: Moerman D Received: 07/01/80 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2105 Species: Caenorhabditis elegans Genotype: let-308(s1705) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Egl, Lethal Twitchers and dead eggs. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Denise Clark Received: 12/12/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2107 Species: Caenorhabditis elegans Genotype: unc-22(s7) unc-31(e169) let-314(s1206)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Egl, UncLet and dead eggs. Lethal at embryo-early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Clark D Received: 01/14/92 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2113 Species: Caenorhabditis elegans Genotype: unc-22(s7) unc-31(e169) let-318(s1218)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, Sterile Uncs and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Clark D Received: 02/10/92 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2120 Species: Caenorhabditis elegans Genotype: unc-22(s7) unc-31(e169) let-321(s1228)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Egl, Sterile Unc, and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Clark D Received: 02/10/92 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2124 Species: Caenorhabditis elegans Genotype: unc-22(s7) unc-31(e169) let-322(s1238)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, UncLets and dead eggs. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Clark D Received: 02/03/92 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2126 Species: Caenorhabditis elegans Genotype: let-657(s1254) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, UncLet, Vul and dead eggs. Maintain by picking twitchers (hets) in 1% nicotine. Mutagen: EMS Outcrossed: x Made by: D. Clark Received: 05/18/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2130 Species: Caenorhabditis elegans Genotype: unc-22(s7) unc-31(e169) let-316(s1227)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul and TwitcherUncLet. Lethal ??. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Denise Clark Received: 09/02/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2137 Species: Caenorhabditis elegans Genotype: let-312(s1234) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, UncLet and dead eggs. Lethal late larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Denise Clark Received: 12/12/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2144 Species: Caenorhabditis elegans Genotype: unc-22(s7) unc-31(e169) let-320(s1248)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul and UncLet. Homozygous s1248 is a sterile adult. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: D. Clark Received: 01/21/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2148 Species: Caenorhabditis elegans Genotype: let-296(s1250) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vuls, early-mid larval lethals which are Unc and Twitch, and dead eggs. Maintain by picking WT. Not outcrossed! Mutagen: EMS Outcrossed: x0 Made by: Denise Clark Received: 03/01/96 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: BC215 Species: Caenorhabditis elegans Genotype: dpy-14(e188) unc-13(e51) let-78(s82)/unc-15(e73) I. Description: Heterozygotes are WT and segregate more WT, paralyzed Unc and DpyUncLethals. The DpyUncs are abnormal larvae which die in late larval development. Pick WT to maintain. Mutagen: EMS Outcrossed: x Made by: Ann Rose Received: 09/01/82 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC2179 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; mDf3/eT1 V. Description: Heterozygotes are WT and segregate WT, dead eggs and Unc-36. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. [2/99: Strain may have broken down: doesn't segregate real WT, and those animals that are not Unc-36 still are somewhat Unc; and unc-23 is no longer under mDf3 in this strain. Mike Nonet] Mutagen: Outcrossed: x Made by: R. Rosenbluth Received: 03/01/87 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC220 Species: Caenorhabditis elegans Genotype: dpy-14(e188) unc-13(e51) let-90(s140)/unc-15(e73) I. Description: Heterozygotes are WT and segregate WT, paralyzed Unc and DpyUncLethal. The DpyUncs are abnormal larvae. Pick WT to maintain. Mutagen: EMS Outcrossed: x Made by: Ann Rose Received: 09/01/82 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC2200 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, dead eggs and DpyUncs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Outcrossed: x Made by: Johnsen RC Received: 12/18/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2237 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-348(s1436) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: R. Johnsen Received: 06/14/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2245 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-339(s1444)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, dead eggs and DpyUncLets. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: R. Johnsen Received: 01/05/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2282 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) pst-1(s1481)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncs which arrest as embryos or as early larva and dead eggs. Mutagen: EMS Outcrossed: x Made by: Bob Johnsen Received: 04/12/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC23 Species: Caenorhabditis elegans Genotype: unc-22(s7) IV. Description: Twitcher Unc. Recessive. Heterozygotes twitch in 1% Nicotine Mutagen: EMS Outcrossed: x Made by: Moerman D Received: 07/01/80 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2303 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) unc-70(s1502)/eT1 V. Description: Heterozygotes are WT and segregate WT, Uncs and DpyUnc Lets that hatch. Mutagen: EMS Outcrossed: x Made by: RC Johnsen Received: 07/03/03 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2383 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-469(s1582)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal as sterile adult. Maintain by picking WT. [7/94: No longer throwing eT1 Unc-36's. May have picked up a lethal mutation on one of the eT1 chromosomes. Also, appears to be lethal in mid-late larval stage rather than as adult.] Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 12/18/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2409 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 [dpy-?] III; let-331(s1608) unc-46(e177)/eT1 [dpy-?] V. Description: Heterozygotes are WT and segregate WT, Unc-36???, Sterile DpyUncs, and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 11/30/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2420 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-346(s1619)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, dead eggs and DpuUncLets. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 05/10/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2422 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-476(s1621)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet (Sterile adults) and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/16/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC244 Species: Caenorhabditis elegans Genotype: dpy-14(e188) let-86(s141) unc-13(e51)/unc-15(e73) I. Description: Heterozygotes are WT and segregate more WT, paralyzed Unc and DpyUncLethals. The DpyUncs are abnormal larvae which die in early larval development. Pick WT to maintain. Mutagen: EMS Outcrossed: x Made by: Rose A Received: 06/01/82 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2440 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) unc-70(s1639)/eT1 V. Description: Heterozygotes are WT and segregate WT, Uncs, and DpyUnc Lets that hatch. Mutagen: EMS Outcrossed: x Made by: RC Johnsen Received: 07/03/03 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2501 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) sDf57/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Co 60 Outcrossed: x1 Made by: R. Rosenbluth Received: 02/17/94 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2504 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-406(s514)/eT1 V. Description: WT strain that segregates WT, Unc-36, dead eggs and mid larval DpyUnc lethals. Maintain by picking WT. Mutagen: Gamma Rays Outcrossed: x Made by: R. Rosenbluth Received: 01/19/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2507 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-60(e677) cdc-25.2(s819) dpy-11(e224)/eT1 V. Description: WT strain which segregates WT, Unc-36, and dead eggs. Egg lethal. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Kim McKim Received: 01/19/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2509 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-60(e677) dpy-11(e224) let-408(s827)/eT1 V. Description: WT strain that segregates WT, Unc-36 and dead eggs. Egg lethal. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Kim McKim Received: 04/28/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2510 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-60(e677) let-426(s826) dpy-11(e224)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Kim McKim Received: 04/15/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2511 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-60(e677) dpy-11(e224) sDf35/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: EMS Outcrossed: x Made by: Mark Heschl Received: 03/01/87 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2513 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; dpy-11(e224) let-407(s830)/eT1 V. Description: WT strain that segregates WT, Unc-36, dead eggs and DpyLets. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Kim McKim Received: 04/28/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2589 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; dpy-11(e224) let-405(s116)/eT1 V. Description: WT strain that segregates WT, Unc-36, dead eggs and DpyLets. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: R. Rosenbluth Received: 04/15/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2590 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; dpy-11(e224) let-404(s119) unc-42(e270)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: T. Rogalski Received: 11/20/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2617 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; mDf1/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Outcrossed: x Made by: Rogalski/Rosenbluth Received: 03/01/87 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2628 Species: Caenorhabditis elegans Genotype: sDf7/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, lethals (early larval) and dead eggs. Hets twitch in 1% nicotine. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Formaldehyde Outcrossed: x Made by: D. Clark Received: 07/17/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2646 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-60(e677) dpy-11(e224) let-410(s815)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Kim McKim Received: 04/15/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2648 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-60(e677) dpy-11(e224) let-409(s823)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Kim McKim Received: 04/28/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2649 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; dpy-11(e224) let-337(s825)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Kim McKim Received: 04/15/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2650 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; dpy-11(e224) rol-3(s833)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, dead eggs, and DpyRol. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Kim McKim Received: 04/28/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2738 Species: Caenorhabditis elegans Genotype: sDf110 dpy-18(e364)/eT1 III; unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36s and dead eggs. Mutagen: UV Outcrossed: x Made by: Received: 08/13/99 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC277 Species: Caenorhabditis elegans Genotype: unc-46(e177) dpy-11(e224) V. Description: DpyUnc. Medium Dpy. Shrinker. Poor backing. Slow, good forward movement. Mutagen: Outcrossed: x6 Made by: Dave Baillie Received: 04/01/85 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2831 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-440(s1411) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLets and dead eggs. Lethal very early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 12/07/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2832 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-336(s1413) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 11/20/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2833 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-442(s1416)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early-mid larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 12/12/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2836 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-436(s1403)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 12/12/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2837 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-326(s1404) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, dead eggs and DpyUncLets. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 12/11/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2838 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-437(s1405) unc-46(e177)/eT1 V. Description: Heterozygotes are WT (abnormal tail on hermpahrodites) and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 12/16/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2840 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-439(s1407)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 12/16/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2841 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-441(s1414)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval--escapers at permissive temp(15C). Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 01/31/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2842 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-444(s1418)/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 12/16/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2851 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-443(s1417)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, dead eggs and DpyUncLets. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 12/16/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2853 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-445(s1419)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet (Mel->larval lethal--tight coiler) and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 12/13/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2858 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-438(s2114)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Maintain by picking WT. Mutagen: Formaldehyde Outcrossed: x Made by: Johnsen RC Received: 12/17/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2890 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-411(s223)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal late larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Raja Rosenbluth Received: 01/19/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2892 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) air-1(s579)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet (Mel->egg lethal) and dead eggs. Maintain by picking WT. Previously called let-412(s579). See also WBPaper00005639. Mutagen: EMS Outcrossed: x Made by: Raja Rosenbluth Received: 01/19/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2895 Species: Caenorhabditis elegans Genotype: let-73(s685) unc-22(s7)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, Twitcher Steriles and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Clark D Received: 01/14/92 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2897 Species: Caenorhabditis elegans Genotype: let-72(s695) unc-22(s7)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, dead eggs, and UncLets. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Teresa Rogalski Received: 01/05/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2898 Species: Caenorhabditis elegans Genotype: let-71(s692) unc-22(s7)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vuls and Lethal Twitchers (Late larval lethals). Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Denise Clark Received: 09/15/97 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2903 Species: Caenorhabditis elegans Genotype: let-92(s504) unc-22(s7)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, dead eggs and UncLets. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Dave Pilgrim Received: 01/05/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2907 Species: Caenorhabditis elegans Genotype: let-91(s678) unc-22(s7)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, mid-larval lethals that Twitch, Vuls and dead eggs. Mutagen: EMS Outcrossed: x Made by: Denise Clark Received: 09/19/97 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2908 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-60(e677) dpy-11(e224) let-423(s818)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Kim McKim Received: 04/28/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2910 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-422(s194) dpy-11(e224) unc-42(e270)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Teresa Roglski Received: 01/19/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2991 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; dpy-11(e224) let-413(s128) unc-42(e270)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Egg lethal. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Teresa Rogalski Received: 01/19/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC2992 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; dpy-11(e224) let-409(s206)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Raja Rosenbluth Received: 12/01/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3005 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-424(s384)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncSterile adults and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Raja Rosenbluth Received: 12/01/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3006 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-330(s1702) unc-46(e177) let-427(s1057)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. let-427 is a sterile adult mutation. let-330 is a mid-larval mutation. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: R. Rosenbluth Received: 06/14/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3016 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; dpy-11(e224) let-416(s113) unc-42(e270)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Late larval lethal. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Teresa Rogalski Received: 12/01/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3017 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; dpy-11(e224) let-414(s114) unc-42(e270)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Teresa Rogalski Received: 12/01/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3019 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; dpy-11(e224) let-415(s129) unc-42(e270)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Mid-late larval lethal. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Teresa Rogalski Received: 12/01/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3020 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; dpy-11(e224) let-408(s195) unc-42(e270)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal late larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Teresa Rogalski Received: 12/01/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3021 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-417(s204) dpy-11(e224) unc-42(e270)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Teresa Rogalski Received: 12/01/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3033 Species: Caenorhabditis elegans Genotype: dpy-18(e346)/eT1 III; sDf48 unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: mut-4 Outcrossed: x Made by: Received: 12/09/92 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3038 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-448(s1363) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal mid-late larval. Maintain by picking WT. Mutagen: Tc1 Outcrossed: x Made by: Clark & McKim Received: 12/17/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3041 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-449(s1343) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. [11/96: the strain is not throwing Unc-36 (eT1 homozygotes). eT1 may have picked up a lethal mutations which causes these animals to arrest, possibly as L1s.] Mutagen: Tc1 Outcrossed: x Made by: Clark & McKim Received: 12/17/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3060 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) mdt-6(s385)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncSte adults and dead eggs. Maintain by picking WT. See also WBPaper00004853. mdt-6 also called med-6 and let-425. See WBPaper00004990. Mutagen: EMS Outcrossed: x Made by: Raja Rosenbluth Received: 12/01/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3062 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-429(s584)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncSte adults and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Raja Rosenbluth Received: 12/01/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3075 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-452(s1434) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval (hatches). Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 12/18/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3076 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-337(s1426)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/11/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3083 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-454(s1423)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal mid-larval; double cuticle, no pumping. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 05/10/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3085 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-453(s2167) let-417(s1424) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: R. Johnsen Received: 06/14/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3087 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-335(s1439)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 01/31/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3097 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-430(s1042) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncSte adults and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: R. Johnsen Received: 12/01/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3099 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-420(s1046) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncSte adult (vulva protrudes) and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen & Rosenbluth Received: 11/20/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3100 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-431(s1049) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncSte adult and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen & Rosenbluth Received: 11/27/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3106 Species: Caenorhabditis elegans Genotype: let-53(s43) unc-22(s7)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and throw WT, TwitcherLets, Vul and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: D. Clark Received: 12/09/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3107 Species: Caenorhabditis elegans Genotype: let-54(s44) unc-22(s7)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate more WT, Vul, and Lethal Twitchers. Lethal early larval. Heterozygotes twitch in 1% Nicotine. Mutagen: EMS Outcrossed: x Made by: Denise Clark Received: 04/30/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3119 Species: Caenorhabditis elegans Genotype: unc-5(e152) unc-22(s7) let-99(s1201) unc-31(e169) IV/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Egl, UncLet (maternal effect lethal) and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Denise Clark Received: 12/12/88 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC313 Species: Caenorhabditis elegans Genotype: rec-1(s180) I. Description: Increased recombination (3X - 4X over WT) for linkage groups I, IV and V. Recessive. Dominant with s155. Mutagen: Outcrossed: x Made by: Rose A Received: 08/01/80 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3134 Species: Caenorhabditis elegans Genotype: srl-2(s2507) dpy-18(e364) III; unc-46(e177) rol-3(s1040) V. Description: srl-2 is a recessive suppressor of the s1040 temperature sensitive lethal phenotype. srl-2 has no effect on the Roller phenotype of s1040. (At 20C, s1040 homozygotes arrest development at mid-larval stage. At 15C, s1040 homozygotes are viable weak left-handed rollers.) Maintain at 20C to select for retention of suppressor. Animals at 20C will be DpyUncRol. Mutagen: EMS Outcrossed: x Made by: W. B. Barbazuk Received: 02/17/94 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3150 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-338(s1020) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen & Rosenbluth Received: 11/27/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3199 Species: Caenorhabditis elegans Genotype: sDf60 IV/nT1 (IV;V). Description: Heterozygotes are WT and segregate WT, Vul and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Formaldehyde Outcrossed: x Made by: Clark D Received: 01/11/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3217 Species: Caenorhabditis elegans Genotype: unc-60(m35) dpy-11(e224) V; sDp30 (V;X). Description: Unc strain. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Gamma Rays Outcrossed: x Made by: Raja Rosenbluth Received: 12/01/89 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3247 Species: Caenorhabditis elegans Genotype: unc-22(s7) unc-31(e169) let-323(s1719)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul and UncLet. Homozygous s1719 are sterile adults. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: D. Clark Received: 01/21/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3255 Species: Caenorhabditis elegans Genotype: unc-22(s7) unc-31(e169) let-324(s1727)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, UncLets and dead eggs. UncLets die in early larval development. Maintain by picking Twitchers in 1% nicotine. Mutagen: EMS Outcrossed: x Made by: D.V. Clark Received: 02/03/94 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3261 Species: Caenorhabditis elegans Genotype: let-653(s1733) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, UncLet and dead eggs. Early larval arrest (L1-L2). Maintain by picking WT. See also WBPaper00002328 and WBPaper00003721. Mutagen: EMS Outcrossed: x Made by: Clark D Received: 01/14/92 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3266 Species: Caenorhabditis elegans Genotype: unc-22(s7) unc-31(e169) let-325(s1738)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul and dead eggs. Mutagen: EMS Outcrossed: x Made by: Diana Collins Received: 07/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3276 Species: Caenorhabditis elegans Genotype: let-655(s1748) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, UncLet (Sterile) and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Clark D Received: 01/14/92 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3282 Species: Caenorhabditis elegans Genotype: unc-22(s7) unc-31(e169) let-319(s1754)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul and UncLet. Lethal late larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: D. Clark Received: 01/21/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3295 Species: Caenorhabditis elegans Genotype: unc-22(s7) let-656(s1767) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul and TwitcherLetUnc. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Denise Clark Received: 09/02/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3303 Species: Caenorhabditis elegans Genotype: egl-38(s1775) unc-22(s7) unc-31(e169) IV/nT1 (IV;V). Description: s1775 homozygotes die as embryos or L1 larvae. Heterozygous animals are WT and segregate WT, Twitcher lethals and dead eggs. nT1 appears to have a lethal mutation. Mutagen: Outcrossed: x Made by: Received: 12/12/97 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: BC331 Species: Caenorhabditis elegans Genotype: let-54(s44) unc-22(s7)/+ + IV. Description: Heterozygotes are WT and segregate WT and Lethal Twitchers. Lethal early larval (L1-L2). Pick heterozygotes to maintain by picking Twitchers in 1% nicotine. Mutagen: EMS Outcrossed: x Made by: Moerman D Received: 12/01/83 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3315 Species: Caenorhabditis elegans Genotype: sDf61 unc-31(e169) IV/nT1 [let-?(m435)] (IV;V). Description: Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Formaldehyde Outcrossed: x Made by: Clark D Received: 01/14/92 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3316 Species: Caenorhabditis elegans Genotype: sDf62 unc-31(e169) IV/nT1 [let-?(m435)] (IV;V). Description: Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Formaldehyde Outcrossed: x Made by: Clark D Received: 01/14/92 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3317 Species: Caenorhabditis elegans Genotype: sDf63 unc-31(e169) IV/nT1 [let-?(m435)] (IV;V). Description: Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Formaldehyde Outcrossed: x Made by: Clark D Received: 02/03/92 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3318 Species: Caenorhabditis elegans Genotype: sDf64 unc-31(e169) IV/nT1 [let-?(m435)] (IV;V). Description: Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Formaldehyde Outcrossed: x Made by: Clark D Received: 02/03/92 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3319 Species: Caenorhabditis elegans Genotype: sDf65 unc-31(e169) IV/nT1 [let-?(m435)] (IV;V). Description: Heterozygotes are WT and segregate WT, lethal Uncs (arrest as L1) and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Formaldehyde Outcrossed: x Made by: Clark D Received: 01/14/92 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3320 Species: Caenorhabditis elegans Genotype: sDf67 unc-31(e169) IV/nT1 [let-?(m435)] (IV;V). Description: Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Formaldehyde Outcrossed: x Made by: Clark D Received: 01/14/92 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3401 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf42 unc-46(e177)/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT, dead eggs, no DpyUnc-46, and no Unc-36. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Formaldehyde Outcrossed: x Made by: Johnsen RC Received: 11/20/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3402 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf32 unc-46(e177)/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT, dead eggs and early larval lethals (sDf32 homozygotes). This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: EMS Outcrossed: x Made by: Rosenbluth Received: 05/23/94 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3413 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-455(s1447) unc-46(e177)/eT1 V. Description: Heterozygottes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 01/31/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3415 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-456(s1479)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 01/23/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3421 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-458(s1443) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 01/23/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3425 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-460(s1664)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: Formaldehyde Outcrossed: x Made by: Johnsen RC Received: 01/23/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3426 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-459(s1432) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 01/31/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3427 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-409(s1480)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval (hatches-tends to curl). Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/11/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3430 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-421(s1477)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, and dead eggs. Egg lethal. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 01/31/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3436 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf33 unc-46(e177)/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT (some seem to look a bit Dpyish) and segregate WT and dead eggs. sDf33 homozygotes arrest as embryos. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Gamma Rays Outcrossed: x Made by: Johnsen Received: 05/23/94 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3439 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf26/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Gamma Rays Outcrossed: x Made by: R. Johnsen Received: 06/29/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3443 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf46 unc-46(e177)/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT, dead eggs, no DpyUnc-46, and no Unc-36. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Formaldehyde Outcrossed: x Made by: Johnsen RC Received: 12/13/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3444 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-419(s1483) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early-mid larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 01/31/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3448 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-428(s1490) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal late larval-Extrudes viscera. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 01/31/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3450 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-403(s1482)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 01/31/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3452 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-463(s2168) rol-3(s1473)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 01/31/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3453 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-464(s1504)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 01/31/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3455 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; lin-40(s1506) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Mid-larval lethal. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 11/15/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3458 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-447(s1457) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet adults (Mel (egg lethal)) and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/11/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3461 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf53 unc-46(e177)/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Formaldehyde Outcrossed: x Made by: Johnsen RC Received: 12/07/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3462 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf39 unc-46(e177)/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Gamma Rays Outcrossed: x Made by: Johnsen RC Received: 11/20/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3463 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf38 unc-46(e177)/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Gamma Rays Outcrossed: x Made by: Johnsen RC Received: 11/27/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3464 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) sDf20/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Gamma Rays Outcrossed: x Made by: R. Johnsen Received: 06/29/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3466 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) sDf30/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Gamma Rays Outcrossed: x Made by: Johnsen/Rosenbluth Received: 06/29/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3468 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) sDf36/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: No mutagen used Outcrossed: x Made by: Rosenbluth/Johnsen Received: 06/29/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3469 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) sDf37/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Gamma Rays Outcrossed: x Made by: Johnsen RC Received: 11/27/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC347 Species: Caenorhabditis elegans Genotype: unc-54(s74) I. Description: Paralyzed Rigid Unc. Muscle birefrigence normal. Muscle normal in EM. Mutagen: EMS Outcrossed: x0 Made by: Moerman D Received: 04/01/80 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: BC3470 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf40 unc-46(e177)/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: mut-4 Outcrossed: x Made by: Johnsen RC Received: 12/10/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3471 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf41 unc-46(e177)/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT, dead eggs, and no Unc-36 or DpyUnc-46. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: mut-4 Outcrossed: x Made by: Johnsen RC Received: 11/29/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3472 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) sDf44/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Formaldehyde Outcrossed: x Made by: Johnsen RC Received: 11/29/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3473 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf45 unc-46(e177)/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: mut-4 Outcrossed: x Made by: Johnsen RC Received: 12/04/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3474 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) sDf47/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Formaldehyde Outcrossed: x Made by: Johnsen RC Received: 11/30/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3476 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf49 unc-46(e177)/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: mut-4 Outcrossed: x Made by: Johnsen RC Received: 12/04/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3499 Species: Caenorhabditis elegans Genotype: let-297(s1989) unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vuls, early larval lethals which are Unc and Twitch, and dead eggs. Maintain by picking WT. Not outcrossed! Mutagen: EMS Outcrossed: x0 Made by: Denise Clark Received: 03/01/96 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: BC352 Species: Caenorhabditis elegans Genotype: let-51(s41) unc-22(s7)/sDf2 IV. Description: Heterozygotes are Twitchers and segregate Twitchers, twitcher progeny that block in egg and dead eggs. Mutagen: Formaldehyde Outcrossed: x Made by: Don Moerman Received: 09/01/82 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3539 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf50 unc-46(e177)/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Formaldehyde Outcrossed: x Made by: Johnsen RC Received: 11/20/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3540 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf51 unc-46(e177)/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT, dead eggs, and no Unc-36 or DpyUnc-46. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: mut-4 Outcrossed: x Made by: Johnsen RC Received: 12/13/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3541 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf52 unc-46(e177)/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: mut-4 Outcrossed: x Made by: Johnsen RC Received: 12/03/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3543 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; nDf18/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Outcrossed: x Made by: Received: 06/29/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3551 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-426(s1527) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 01/31/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3555 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-467(s1521)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval (hatches). Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 01/31/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3557 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-340(s1508)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/11/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3559 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-415(s1525) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/11/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3564 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-327(s1496) unc-46(e177)/eT1 V. Description: Hets are Slow, have Morphological Abnormalities, and are lethal at 25C. Segregate hets, Unc-36 dead eggs, and DpyUncLet. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/11/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3581 Species: Caenorhabditis elegans Genotype: eT1 unc-60(s1331) III; eT1 V. Description: Unc-paralysed. Both Unc-60 and Unc-36. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Outcrossed: x Made by: Raja Rosenbluth Received: 07/16/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3590 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-433(s950)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUnc sterile adults and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: H. Stewart Received: 02/17/94 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3592 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-434(s1904)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUnc sterile adults and dead eggs. Maintain by picking WT. Mutagen: Gamma Rays Outcrossed: x Made by: H. Stewart Received: 02/17/94 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC364 Species: Caenorhabditis elegans Genotype: dpy-11(e224) unc-68(e540) V. Description: DpyUnc. Mutagen: Outcrossed: x Made by: R. Rosenbluth Received: 04/01/85 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3655 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-468(s1533)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/11/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3670 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; lag-2(s1486) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval, mid-larval at 15C. Maintain by picking WT. Previously called let-461(s1486). Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/11/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3674 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-470(s1581)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/11/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3684 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-472(s1605)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/12/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC369 Species: Caenorhabditis elegans Genotype: unc-5(e152) let-55(s45) unc-22(s7)/unc-5(e152) IV. Description: Heterozygotes are Unc and segregate Unc and Lethal Unc Twitchers. Lethal early larval. Heterozygotes twitch in 1% nicotine. Mutagen: EMS Outcrossed: x Made by: Moerman D Received: 12/01/83 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3698 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-411(s1595)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal mid-larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/19/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3700 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) pst-1(s1594)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal embryo to early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/11/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3702 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-402(s1526)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/11/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3712 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-474(s1577)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/11/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3713 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-473(s1602)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. [This strain is acting strangely: it is throwing bent heads. 12/95] Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/12/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3716 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-475(s1606)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet (Sterile adult) and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/20/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3718 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-407(s1631)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval (hatches). Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/16/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3727 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf56 unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/19/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3728 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-480(s1607)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet (Sterile adult) and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 05/10/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3729 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-478(s1620) let-?(s2169) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/18/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3731 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-479(s1576)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet (Sterile adult-> Lays unfertilized eggs) and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/18/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3732 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-418(s1617)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet (Sterile adult; Vulva protrudes; partial rescue at 15C) and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/18/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3733 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; let-481(s1636) unc-46(e177)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval (hatches). Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Johnsen RC Received: 02/27/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3737 Species: Caenorhabditis elegans Genotype: sDp8 (III;I); eT1 (III;V). Description: WT strain. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Outcrossed: x Made by: Stewart H Received: 05/10/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3840 Species: Caenorhabditis elegans Genotype: sDf69(s2298) unc-22(s7) lev-1(x22)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul and dead eggs. Hets twitch in 1% nicotine. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: EMS Outcrossed: x Made by: Marco Marra Received: 05/27/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3953 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf70 unc-46(e177)/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT, no Unc36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: UV Outcrossed: x Made by: Stewart H Received: 10/15/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3954 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) sDf71/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT and dead eggs. Embryonic lethal. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: UV Outcrossed: x Made by: Stewart H Received: 10/15/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3955 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf72 unc-46(e177)/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT and dead eggs. Embryonic lethal. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: UV Outcrossed: x Made by: Stewart H Received: 10/15/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3956 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf73 unc-46(e177)/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT, dead eggs, no Unc-36, and DpyUncLet. Lethal early larva. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: UV Outcrossed: x Made by: Stewart H Received: 04/29/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3957 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf74 unc-46(e177)/eT1 [let-500(s2165)] V. Description: WT strain which segregates WT and dead eggs. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: UV Outcrossed: x Made by: Stewart H Received: 10/15/90 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3958 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf75 unc-46(e177)/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT and dead eggs. Embryonic lethal. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: UV Outcrossed: x Made by: Stewart H Received: 04/29/91 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC3972 Species: Caenorhabditis elegans Genotype: srl-2(s2508) dpy-18(e354) III. Description: Dpy. Mutagen: EMS Outcrossed: x Made by: W. B. Barbazuk Received: 04/12/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4006 Species: Caenorhabditis elegans Genotype: sDf83 IV/nT1 [let-?(m435)] (IV;V). Description: sDf83 homozygotes arrest as late larva or sterile adults after 2 weeks. Mutagen: Formaldehyde Outcrossed: x Made by: J. Schein Received: 09/05/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4008 Species: Caenorhabditis elegans Genotype: sDf85(s2089) IV/nT1 [let-?(m435)] (IV;V). Description: Heterozygotes are WT and segregate WT and dead eggs. sDf85 homozygotes arrest as embryos or early L1 larva. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Formaldehyde Outcrossed: x Made by: J. Schein Received: 05/27/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4011 Species: Caenorhabditis elegans Genotype: srl-1(s2500) II; dpy-18(e364) III; unc-46(e177) rol-3(s1040) V. Description: srl-1 is a recessive suppressor of the s1040 temperature sensitive lethal phenotype. srl-1 has no effect on the Roller phenotype of s1040. (At 20C, s1040 homozygotes arrest development at mid-larval stage. At 15C, s1040 homozygotes are viable weak left-handed rollers.) Maintain at 20C to select for retention of suppressor. Animals at 20C will be DpyUncRol. Mutagen: Gamma Rays Outcrossed: x1 Made by: W. B. Barbazuk Received: 02/17/94 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4095 Species: Caenorhabditis elegans Genotype: sDf88(s2074) IV. Description: PCR used to establish that s2074 is a deletion. Twitcher. Mutagen: Formaldehyde Outcrossed: x Made by: J. Schein Received: 03/09/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4142 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-769(s2432) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in mid to late larval development. Mutagen: EMS Outcrossed: x Made by: A.M. Howell Received: 08/07/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4143 Species: Caenorhabditis elegans Genotype: dpy-17(e164) mup-4(s2433) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in early larval development. s2433 previously assigned to let-701. Mutagen: EMS Outcrossed: x Made by: Howell & Stewart Received: 06/28/99 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4144 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-723(s2434) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and Sterile as adults. Mutagen: EMS Outcrossed: x Made by: Howell & Stewart Received: 04/23/03 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4145 Species: Caenorhabditis elegans Genotype: let-738(s2435) dpy-17(e164) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication arrest as embryos. Mutagen: EMS Outcrossed: x Made by: Howell and Stewart Received: 10/06/99 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4148 Species: Caenorhabditis elegans Genotype: dpy-17(e164) mel-31(s2438) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and give only dead eggs. Mutagen: EMS Outcrossed: x Made by: A.M. Howell Received: 09/05/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4149 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-712(s2439) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs arrest as early larvae. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4151 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-972(s2441) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs arrest at early larvae. s2441 previously assigned to let-703. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4152 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-771(s2442) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest as Sterile Adults. Mutagen: EMS Outcrossed: x Made by: A. Howell Received: 07/13/99 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4153 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-764(s2443) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest as early larva. s2443 pka let-708. Mutagen: EMS Outcrossed: x Made by: A.M. Howell Received: 08/07/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4154 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-709(s2444) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs die as embryos. This is a slow developing strain. Mutagen: EMS Outcrossed: x Made by: A.M. Howell Received: 06/12/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4155 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-718(s2445) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs arrest as early larvae. This is a slow developing strain. Received new stock 9/03. 3/09: Buelow lab reported not seeing lethal DpyUncs. Mutagen: EMS Outcrossed: x Made by: A.M. Howell Received: 06/12/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4156 Species: Caenorhabditis elegans Genotype: let-750(s2446) dpy-17(e164) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs arrest as early larvae. This is a slow developing strain. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4157 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-721(s2447) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUncs and are sterile adults. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 09/16/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4159 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-713(s2449) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUncs and arrest at the mid-larval stage of development. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 09/16/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4163 Species: Caenorhabditis elegans Genotype: let-776(s2453) dpy-17(e164) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest at a mid-larval stage of development. Mutagen: EMS Outcrossed: x Made by: A.M. Howell Received: 05/12/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4164 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-725(s2454) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUncs and are sterile adults (some lay eggs that don't hatch). Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 09/16/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4165 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-719(s2455) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest as Sterile Adults. Mutagen: EMS Outcrossed: x Made by: A. Howell Received: 07/13/99 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4166 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-747(s2456) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs arrest at early to mid larval stage. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4167 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-716(s2457) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUncs which arrest at an early larval stage. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 09/16/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4168 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-704(s2458) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in mid-late larval development. Mutagen: EMS Outcrossed: x Made by: Howell & Stewart Received: 06/28/99 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4171 Species: Caenorhabditis elegans Genotype: let-761(s2461) dpy-17(e164) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs arrest at an early larval stage. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4172 Species: Caenorhabditis elegans Genotype: let-707(s2462) dpy-17(e164) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest at the early larval stage. Mutagen: EMS Outcrossed: x Made by: A. Howell Received: 03/02/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4173 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-766(s2463) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest as early larva. Mutagen: EMS Outcrossed: x Made by: A.M. Howell Received: 08/07/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4179 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-777(s2469) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and become sterile adults. Mutagen: EMS Outcrossed: x Made by: A.M. Howell Received: 04/12/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4181 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-717(s2471) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs arrest as early larvae. Mutagen: EMS Outcrossed: x Made by: A.M. Howell Received: 06/12/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4185 Species: Caenorhabditis elegans Genotype: let-763(s2475) dpy-17(e164) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in early larval development. Mutagen: EMS Outcrossed: x Made by: A.M. Howell Received: 08/07/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4186 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-741(s2476) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs arrest at early to mid larval stage. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4187 Species: Caenorhabditis elegans Genotype: let-737(s2477) dpy-17(e164) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs arrest as early larvae. Mutagen: EMS Outcrossed: x Made by: A.M. Howell Received: 06/12/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4188 Species: Caenorhabditis elegans Genotype: let-700(s2478) dpy-17(e164) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest at an early larval stage. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 04/16/01 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4190 Species: Caenorhabditis elegans Genotype: let-706(s2480) dpy-17(e164) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest at an early larval stage. Mutagen: EMS Outcrossed: x Made by: A.M. Howell Received: 05/12/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4192 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-768(s2482) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest as sterile adults. Mutagen: EMS Outcrossed: x Made by: Howell & Stewart Received: 06/28/99 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4212 Species: Caenorhabditis elegans Genotype: let-710(s2572) dpy-17(e164) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest at an early larval stage, but s2572 is leaky. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 04/16/01 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4213 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-728(s2573) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs arrest at early-mid larval stage. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4216 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-780(s2576) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest as Sterile Adults. Mutagen: EMS Outcrossed: x Made by: A. Howell Received: 07/13/99 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4218 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-748(s2578) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Mutagen: EMS Outcrossed: x Made by: A. Howell Received: 07/13/99 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4219 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-729(s2579) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and are embyronic lethals. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 04/16/01 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4222 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-714(s2582) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs arrest as early larvae. s2582 previously thought to be an allele of let-726. Mutagen: EMS Outcrossed: x Made by: A.M. Howell Received: 06/12/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4224 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-778(s2584) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest at the early larval stage. Mutagen: EMS Outcrossed: x Made by: A. Howell Received: 03/02/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4225 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-786(s2585) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in the early larval stage. Mutagen: EMS Outcrossed: x Made by: A.M. Howell Received: 09/05/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4227 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-711(s2587) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs arrest as early larvae. Mutagen: EMS Outcrossed: x Made by: A.M. Howell Received: 06/12/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4229 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-727(s2589) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest at an early larval stage. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 04/16/01 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4231 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-782(s2591) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs become sterile adults. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4233 Species: Caenorhabditis elegans Genotype: let-739(s2593) dpy-17(e164) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs arrest at early-mid larval stage. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4235 Species: Caenorhabditis elegans Genotype: let-759(s2595) dpy-17(e164) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest as late larvae or sterile adults. Mutagen: EMS Outcrossed: x Made by: A.M. Howell Received: 08/14/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4237 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-844(s2597) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs arrest at early larval stage. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4241 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-783(s2601) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and are probably embyronic lethals. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 04/16/01 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4242 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-784(s2602) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs become sterile adults. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4243 Species: Caenorhabditis elegans Genotype: let-760(s2603) dpy-17(e164) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in late larval development. Mutagen: EMS Outcrossed: x Made by: A.M. Howell Received: 08/07/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4245 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-753(s2605) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs arrest at early larval stage. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4246 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-789(s2606) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and become sterile adults. Mutagen: EMS Outcrossed: x Made by: A. Howell Received: 03/02/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4247 Species: Caenorhabditis elegans Genotype: let-758(s2607) dpy-17(e164) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc are adult steriles. Mutagen: EMS Outcrossed: x Made by: A.M. Howell Received: 08/07/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4249 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-746(s2609) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in early larval stages. Mutagen: EMS Outcrossed: x Made by: Howell & Stewart Received: 06/28/99 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4253 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-756(s2613) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUncs which arrest as early larvae. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 09/16/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4255 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-774(s2615) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUncs which arrest as early larvae. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 09/16/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4256 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-764(s2616) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest at the early larval stage. Mutagen: EMS Outcrossed: x Made by: A. Howell Received: 03/02/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4258 Species: Caenorhabditis elegans Genotype: let-715(s2618) dpy-17(e164) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs become sterile adults. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4259 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-752(s2619) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest as sterile adults. Mutagen: EMS Outcrossed: x Made by: Howell & Stewart Received: 06/28/99 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4261 Species: Caenorhabditis elegans Genotype: let-733(s2621) dpy-17(e164) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest as Sterile Adults. Note: let-733(s2621) seems to interact with let-749(s2467). Mutagen: EMS Outcrossed: x Made by: A. Howell Received: 07/13/99 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4262 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-754(s2622) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in mid larval development. Mutagen: EMS Outcrossed: x Made by: A.M. Howell Received: 08/07/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4267 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-843(s2627) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. After 3 days at 20C the DpyUncs are at the mid-larvae stage; by day 10 they are adults giving some eggs which hatch sick looking larvae. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4270 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-765(s2630) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs arrest at mid-larval stage. s2630 pka let-745. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4271 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-786(s2631) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest at the early larval stage. s2631 previously thought to be an allele of let-731. Mutagen: EMS Outcrossed: x Made by: A. Howell Received: 03/02/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4279 Species: Caenorhabditis elegans Genotype: sC1(s2023) [dpy-1(s2170)] III. Description: Dpy. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: EMS Outcrossed: x Made by: A.M. Howell Received: 05/27/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4289 Species: Caenorhabditis elegans Genotype: sDp10 (IV;X). Description: Mutagen: Gamma Rays Outcrossed: x Made by: M.A. Marra Received: 09/05/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC439 Species: Caenorhabditis elegans Genotype: sDf7/unc-31(e169) dpy-4(e1166) IV. Description: Heterozygotes are WT and segregate DpyUncs and early larval lethals. Heterozygotes twitch in 1% Nicotine. Pick WT to maintain. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Formaldehyde Outcrossed: x Made by: Moerman D Received: 06/01/82 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4390 Species: Caenorhabditis elegans Genotype: sEx776. Description: sEx776 [C05A2 (V) + pCes1943[rol-6(su1006)]]. "Low" amount of cosmid C05A2 + 100 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: The Ha Received: 04/15/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC442 Species: Caenorhabditis elegans Genotype: sDf10/unc-31(e169) IV. Description: Heterozygotes twitch in 1% nicotine. Hets are slow, and are somewhat difficult to distinguish from unc-31 homozygotes. Df/Df homozygotes die as early larvae. Maintain by picking twitchers in 1% nicotine. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Formaldehyde Outcrossed: x Made by: Moerman D Received: 06/01/82 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4425 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; sDf48 unc-46(e177)/eT1 [let-500(s2165)] V. Description: Heterozygotes are WT and segregate WT and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: mut-4 Outcrossed: x Made by: D. Clark Received: 03/04/93 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4542 Species: Caenorhabditis elegans Genotype: sEx29. Description: sEx29 [ZK652 (III) + pCes1943[rol-6(su1006)]]. Cosmid ZK652 and plasmid pCes1943 were injected into hermaphrodite from N2 strain. pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4544 Species: Caenorhabditis elegans Genotype: sEx31. Description: sEx31 [ZK688 (III) + pCes1943[rol-6(su1006)]]. Cosmid ZK688 and plasmid pCes1943 were injected into hermaphrodite from N2 strain. pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collin Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4545 Species: Caenorhabditis elegans Genotype: sEx32. Description: sEx32 [T20H4 (III) + pCes1943[rol-6(su1006)]]. T20H4 + pCes1943[rol-6(su1006dm)] injected into N2 hermaphrodite. pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC455 Species: Caenorhabditis elegans Genotype: unc-15(e73) unc-13(e51) I. Description: Paralysed Unc. Mutagen: Outcrossed: x Made by: Ann Rose Received: 06/12/87 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: BC4566 Species: Caenorhabditis elegans Genotype: sEx34. Description: sEx34 [T21D11 (III) + pCes1943[rol-6(su1006)]]. Cosmid T21D11 + pCes1943[rol-6(su1006dm)] injected into N2 hermaphrodite. pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4583 Species: Caenorhabditis elegans Genotype: sEx37. Description: sEx37 [C06G4 (III) + pCes1943[rol-6(su1006)]]. Cosmid C06G4 and plasmid pCes1943 were injected into hermaphrodite from N2 strain. pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4585 Species: Caenorhabditis elegans Genotype: sEx39. Description: sEx39 [K06H7 (III) + pCes1943[rol-6(su1006)]]. Cosmid K06H7 and plasmid pCes1943 were injected into hermaphrodite from N2 strain. pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4586 Species: Caenorhabditis elegans Genotype: unc-76(e911) rol-9(sc148)/sC4(s2172) [dpy-21(e428)] V. Description: Heterozygotes are WT and segregate WT and Unc Rollers. sC4(s2172) is not viable as a homozygote. As a heterozygote it reduces recombination between unc-76 and rol-9 to 1.8%. Note: This strain has been sequenced and the sC4 balancer contains a large deletion from V:16,060,619 to V:19,331,432 that removes 1,279 genes and has a complex rearrangement on LGIV. See Maroilley et al. Sci Reports (2021)11:18258 for more details. doi.org/10.1038/s41598-021-97764-9 Mutagen: Gamma Rays Outcrossed: x Made by: Helen Stewart Received: 12/10/01 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4604 Species: Caenorhabditis elegans Genotype: sEx50. Description: sEx50 [F44B9 (III) + pCes1943[rol-6(su1006)]]. 20 ng/ul F44B9 + 110 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4634 Species: Caenorhabditis elegans Genotype: dpy-17(e164) sDf125(s2424) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Unc-32 animals. Mutagen: UV Outcrossed: x Made by: Helen Stewart Received: 08/31/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4637 Species: Caenorhabditis elegans Genotype: sDf130(s2427) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Unc-32 animals. Mutagen: UV Outcrossed: x Made by: Helen Stewart Received: 08/31/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4638 Species: Caenorhabditis elegans Genotype: dpy-17(e164) sDf127(s2428) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Unc-32 animals. Mutagen: UV Outcrossed: x Made by: Helen Stewart Received: 08/31/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4649 Species: Caenorhabditis elegans Genotype: sEx67. Description: sEx67 [R151 (III) + pCes1943[rol-6(su1006)]]. segrgnt. 1. 20 ng/ul R151 + 110 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4650 Species: Caenorhabditis elegans Genotype: sEx68. Description: sEx68 [R13A5 (III) + pCes1943[rol-6(su1006)]]. 20 ng/ul R13A5 + 110 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4652 Species: Caenorhabditis elegans Genotype: sEx70. Description: sEx70 [T20B12 (III) + pCes1943[rol-6(su1006)]]. segrgnt 2. 20 ng/ul T20B12 + 110 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4655 Species: Caenorhabditis elegans Genotype: sEx73. Description: sEx73 [F08F8 (III) + pCes1943[rol-6(su1006)]]. segrgnt 3. 20 ng/ul F08F8 + 110 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4660 Species: Caenorhabditis elegans Genotype: sEx76. Description: sEx76 [C30C5 (III) + pCes1943[rol-6(su1006)]]. 20 ng/ul C30C5 + 110 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4662 Species: Caenorhabditis elegans Genotype: sEx77. Description: sEx77 [F56C9 (III) + pCes1943[rol-6(su1006)]]. segrgnt 1. 20 ng/ul F56C9 + 110 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4665 Species: Caenorhabditis elegans Genotype: sEx81. Description: sEx81 [F37C12 (III) + pCes1943[rol-6(su1006)]]. segrgnt 5. 20 ng/ul F37C12 + 110 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4666 Species: Caenorhabditis elegans Genotype: sEx82. Description: sEx82 [T04A6 (III) + pCes1943[rol-6(su1006)]]. segrgnt 1. 20 ng/ul T04A6 + 110 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4667 Species: Caenorhabditis elegans Genotype: sEx83. Description: sEx83 [C18H2 (III) + pCes1943[rol-6(su1006)]]. segrgnt. 1. 20 ng/ul C18H2 + 110 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4677 Species: Caenorhabditis elegans Genotype: dpy-17(e164) sDf135(s2767) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Unc-32 animals. DpyUncs arrest development at early larval stages. Mutagen: Gamma Rays Outcrossed: x Made by: Helen Stewart Received: 08/31/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4694 Species: Caenorhabditis elegans Genotype: sEx95. Description: sEx95 [C14B9 (III) + pCes1943[rol-6(su1006)]]. 20 ng/ul C14B9 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4696 Species: Caenorhabditis elegans Genotype: dpy-17(e164) sDf128(s2786) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Unc-32 animals. Mutagen: Gamma Rays Outcrossed: x Made by: Helen Stewart Received: 08/31/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4697 Species: Caenorhabditis elegans Genotype: sDf121(s2098) unc-32(e189) III; sDp3 (III;f). Description: Unc strain. Aged: Original sDf121 strain (BC4201) was maintained on plates for approx. 2 years, and then frozen as BC4697. See also WBPaper00003827. Mutagen: Gamma Rays Outcrossed: x Made by: A. Howell Received: 07/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4699 Species: Caenorhabditis elegans Genotype: sEx90. Description: sEx90 [D2007 (III) + pCes1943[rol-6(su1006)]]. segrgnt. 5. 20 ng/ul D2007 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4700 Species: Caenorhabditis elegans Genotype: sEx91. Description: sEx91 [C29E4 (III) + pCes1943[rol-6(su1006)]]. segrgnt. 1. 20 ng/ul C29E4 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4738 Species: Caenorhabditis elegans Genotype: sEx778. Description: sEx778 [B0379 (I) + pCes1943[rol-6(su1006)]]. line 1. "Low" amount of cosmid B0379 + 100 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: The Ha Received: 04/15/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4785 Species: Caenorhabditis elegans Genotype: sEx114. Description: sEx114 [C06E8 (III) + pCes1943[rol-6(su1006)]]. segrgnt. 2. 10.6 ng/ul C06E8 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4787 Species: Caenorhabditis elegans Genotype: sEx111. Description: sEx111 [F11H8 (III) + pCes1943[rol-6(su1006)]]. segrgnt 4. 20 ng/ul F11H8 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4798 Species: Caenorhabditis elegans Genotype: sEx119. Description: sEx119 [K04C2 (III) + pCes1943[rol-6(su1006)]]. 20 ng/ul K04C2 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4799 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-724(s2437) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Howell/Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4816 Species: Caenorhabditis elegans Genotype: sEx121. Description: sEx121 [R13F6 (III) + pCes1943[rol-6(su1006)]]. 20 ng/ul R13F6 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4822 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-823(s2792) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4823 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-824(s2793) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4824 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-812(s2794) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4825 Species: Caenorhabditis elegans Genotype: dpy-17(e164) dcr-1(s2795) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. s2795 previously called let-740. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4826 Species: Caenorhabditis elegans Genotype: let-811(s2796) dpy-17(e164) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4827 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-828(s2797) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4828 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-792(s2798) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4829 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-815(s2799) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4831 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-813(s2801) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4832 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-835(s2802) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4833 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-836(s2803) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4834 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-821(s2804) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4835 Species: Caenorhabditis elegans Genotype: mel-33(s2805) dpy-17(e164) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs give eggs that don't develop into adults. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4836 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-825(s2806) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4838 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-732(s2808) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4841 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-826(s2811) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4843 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-702(s2813) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4844 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-841(s2814) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4845 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-834(s2815) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4846 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-722(s2816) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4847 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-832(s2817) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4848 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-816(s2818) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4849 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-767(s2819) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Animals with the duplication are Unc. See also WBPaper00002901. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 06/07/00 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4850 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-826(s2820) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4851 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-839(s2821) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4852 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-796(s2822) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4853 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-817(s2823) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4854 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-797(s2824) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4855 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-793(s2825) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4856 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-829(s2826) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4857 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-827(s2827) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4858 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-830(s2828) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4859 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-814(s2829) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4860 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-743(s2830) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4862 Species: Caenorhabditis elegans Genotype: let-736(s2832) dpy-17(e164) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4863 Species: Caenorhabditis elegans Genotype: dpy-17(e164) cyk-1(s2833) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. cyk-1 was previously known as let-794 (in Stewart et al 1998). Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4864 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-837(s2834) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4865 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-818(s2835) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4866 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-810(s2836) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4867 Species: Caenorhabditis elegans Genotype: let-734(s2837) dpy-17(e164) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. pka let-833(s2837). Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4868 Species: Caenorhabditis elegans Genotype: let-791(s2838) dpy-17(e164) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4869 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-838(s2839) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4870 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-775(s2840) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4873 Species: Caenorhabditis elegans Genotype: let-819(s2843) dpy-17(e164) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4874 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-809(s2844) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4875 Species: Caenorhabditis elegans Genotype: let-820(s2845) dpy-17(e164) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4876 Species: Caenorhabditis elegans Genotype: let-840(s2846) dpy-17(e164) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4878 Species: Caenorhabditis elegans Genotype: let-822(s2848) dpy-17(e164) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4879 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-795(s2849) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4880 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-798(s2850) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs die as embryos. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4881 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-842(s2851) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4882 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-799(s2852) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 10/28/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4883 Species: Caenorhabditis elegans Genotype: let-831(s2853) dpy-17(e164) ncl-1(e1865) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. Mutagen: EMS Outcrossed: x Made by: Helen Stewart Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4885 Species: Caenorhabditis elegans Genotype: sEx124. Description: sEx124 [K07E12 (III) + pCes1943[rol-6(su1006)]]. segrgnt 2. 20 ng/ul K07E12 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4890 Species: Caenorhabditis elegans Genotype: sEx126. Description: sEx126 [K03F8 (III) + pCes1943[rol-6(su1006)]]. segrgnt 2. 20 ng/ul K03F8 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4892 Species: Caenorhabditis elegans Genotype: sEx128. Description: sEx128 [M01G4 (III) + pCes1943[rol-6(su1006)]]. 20 ng/ul M01G4 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4909 Species: Caenorhabditis elegans Genotype: sEx134. Description: sEx134 [F42A10 (III) + pCes1943[rol-6(su1006)]]. segrgnt 2. 20 ng/ul F42A10 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4913 Species: Caenorhabditis elegans Genotype: sEx136. Description: sEx136 [F28F5 (III) + pCes1943[rol-6(su1006)]]. 10ng/ul mini-prepped F28F5 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4914 Species: Caenorhabditis elegans Genotype: sEx137. Description: sEx137 [C05D11 (III) + pCes1943[rol-6(su1006)]]. segrgnt 3. 20 ng/ul C05D11 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 08/31/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4915 Species: Caenorhabditis elegans Genotype: sEx138. Description: sEx138 [K06G1 (I) + PCes1943[rol-6(su1006)]]. segrgnt. 2. 5ng/ul mini-prepped K06G1 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4916 Species: Caenorhabditis elegans Genotype: sEx139. Description: sEx139 [F44E2 (III) + pCes1943[rol-6(su1006)]]. 20 ng/ul F44E2 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4923 Species: Caenorhabditis elegans Genotype: egl-38(sy294) IV; him-5(e1490) V. Description: Mutagen: Outcrossed: x Made by: Received: 12/30/97 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: BC4925 Species: Caenorhabditis elegans Genotype: sEx142. Description: sEx142 [T12A2 (III) + pCes1943[rol-6(su1006)]]. segrgnt. 2. 10 ng/ul T12A2 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4926 Species: Caenorhabditis elegans Genotype: sEx143. Description: sEx143 [T12A2 (III) + pCes1943[rol-6(su1006)]]. segrgnt 3. 10 ng/ul T12A2 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 08/31/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4927 Species: Caenorhabditis elegans Genotype: sEx144. Description: sEx144 [C23G10 (III) + pCes1943[rol-6(su1006)]]. segrgnt 1. 20 ng/ul C23G10 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4929 Species: Caenorhabditis elegans Genotype: sEx146. Description: sEx146 [C05D10 (III) + pCes1943[rol-6(su1006)]]. segrgnt. 1. 20 ng/ul C05D10 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4931 Species: Caenorhabditis elegans Genotype: sEx148. Description: sEx148 [C05D11 (III) + pCes1943[rol-6(su1006)]]. segrgnt 5. 20 ng/ul C05D11 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4932 Species: Caenorhabditis elegans Genotype: sEx149. Description: sEx149 [F56D2 (III) + pCes1943[rol-6(su1006)]]. 20 ng/ul FF56D2 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 04/15/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4936 Species: Caenorhabditis elegans Genotype: sEx152. Description: sEx152 [C05D2 (III) + pCes1943[rol-6(su1006)]]. 20 ng/ul C05D2 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4964 Species: Caenorhabditis elegans Genotype: sEx164. Description: sEx164 [ZK418 (III) + pCes1943[rol-6(su1006)]]. 8 ng/ul mini-prepped cosmid ZK418 + 100 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4967 Species: Caenorhabditis elegans Genotype: sEx167. Description: sEx167 [R02F2 (III) + pCes1943[rol-6(su1006)]]. 9 ng/ul R02F2 + 91 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4970 Species: Caenorhabditis elegans Genotype: sEx170. Description: sEx170 [ZC155 (III) + pCes1943[rol-6(su1006)]]. 20 ng/ul ZC155 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4971 Species: Caenorhabditis elegans Genotype: sEx171. Description: sEx171 [T26A5 (III) + pCes1943[rol-6(su1006)]]. segrgnt 1. 10 ng/ul T26A5 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC4997 Species: Caenorhabditis elegans Genotype: dpy-17(e164) let-755(s2861) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Uncs. DpyUncs arrest at early-mid larval stages. Mutagen: EMS Outcrossed: x Made by: Greg Vatcher Received: 11/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5022 Species: Caenorhabditis elegans Genotype: sEx190. Description: sEx190 [F58A4 (III) + pCes1943[rol-6(su1006)]]. 20 ng/ul F54A8 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5025 Species: Caenorhabditis elegans Genotype: sEx193. Description: sEx193 [R05D3 (III) + pCes1943[rol-6(su1006)]]. 4 ng/ul mini-prepped cosmid R05D3 + 100 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5026 Species: Caenorhabditis elegans Genotype: sEx194. Description: sEx194 [R10E11 + pCes1943[rol-6(su1006)]]. 20 ng/ul R10E11 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5038 Species: Caenorhabditis elegans Genotype: sEx206. Description: sEx206 [ZK637 (III) + pCes1943[rol-6(su1006)]]. segrgnt 2. 6 ng/ul ZK637 + 100 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5055 Species: Caenorhabditis elegans Genotype: sEx223. Description: sEx223 [BO336 (III) + pCes1943[rol-6(su1006)]]. segrgnt A. 10 ng/ul BO336 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5058 Species: Caenorhabditis elegans Genotype: sEx226. Description: sEx226 [C13B9 (III) + pCes1943[rol-6(su1006)]]. segrgnt C. 20 ng/ul CB13B9 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5062 Species: Caenorhabditis elegans Genotype: sEx230. Description: sEx230 [F57B9 (III) + pCes1943[rol-6(su1006)]]. segrgnt. C. 5 ng/ul F57B9 + 95 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5063 Species: Caenorhabditis elegans Genotype: sEx264. Description: sEx264 [K04G7 (III) + pCes1943[rol-6(su1006)]]. segrgnt A. 10 ng/ul K04G7 + 100 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5078 Species: Caenorhabditis elegans Genotype: dpy-17(e164) mel-32(s2518) unc-32(e189) III; sDp3 (III;f). Description: Maintain by picking Unc-32 animals. Dpy animals that have lost sDp3 should also be Mel; expected Mel phenotype is embryonic lethal. Mutagen: EMS Outcrossed: x Made by: Greg Vatcher Received: 08/31/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5085 Species: Caenorhabditis elegans Genotype: sEx232. Description: sEx232 [B0303 (III) + pCes1943[rol-6(su1006)]]. segrgnt. 2. 5 ng/ul mini-prepped B0303 + 95 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5088 Species: Caenorhabditis elegans Genotype: sEx235. Description: sEx235 [R01H2 (III) + pCes1943[rol-6(su1006)]]. segrgnt. 3. 7 ng/ul R01H2 + 86 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5092 Species: Caenorhabditis elegans Genotype: sEx239. Description: sEx239 [BO361 (III) + pCes1943[rol-6(su1006)]]. segrgnt 2. A small amount of cosmid BO361 + 100 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5102 Species: Caenorhabditis elegans Genotype: sEx249. Description: sEx249 [T07E3 (III) + pCes1943[rol-6(su1006)]]. segrgnt. 3. 20 ng/ul T07E3 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5108 Species: Caenorhabditis elegans Genotype: sEx255. Description: sEx255 [pCes1943[rol-6(su1006)]]. 100 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5122 Species: Caenorhabditis elegans Genotype: sEx270. Description: sEx270 [ZK783 (III) + pCes1943[rol-6(su1006)]]. segrgnt C. Unknown amount of cosmid ZK783 + 100 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5123 Species: Caenorhabditis elegans Genotype: sEx271. Description: sEx271 [F54D8 (III) + pCes1943[rol-6(su1006)]]. segrgnt D. 20 ng/ul F54D8 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5124 Species: Caenorhabditis elegans Genotype: sEx272. Description: sEx272 [R12B2 (III) + pCes1943[rol-6(su1006)]]. 8.2 ng/ul R12B2 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5125 Species: Caenorhabditis elegans Genotype: sEx273. Description: sEx273 [F42G9 (III) + pCes1943[rol-6(su1006)]]. segrgnt 1. 20 ng/ul F42G9 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5127 Species: Caenorhabditis elegans Genotype: sEx275. Description: sEx275 [C31H11 (III) + pCes1943[rol-6(su1006)]]. segrgnt. 1. 10 ng/ul mini-prepped C31H11 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5155 Species: Caenorhabditis elegans Genotype: sEx282. Description: sEx282 [F01F1 (III) + pCes1943[rol-6(su1006)]]. segrgnt 1. 10 ng/ul F01F1 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5164 Species: Caenorhabditis elegans Genotype: sEx291. Description: sEx291 [F54E7 (III) + pCes1943[rol-6(su1006)]]. segrgnt. J. 20 ng/ul F54E7 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 01/20/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5173 Species: Caenorhabditis elegans Genotype: sEx293. Description: sEx293 [C07D10 (II) + pCes1943[rol-6(su1006)]]. segrgnt 1. 20 ng/ul C07D10 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 01/30/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5206 Species: Caenorhabditis elegans Genotype: sEx317. Description: sEx317 [F21H11 (III) + pCes1943[rol-6(su1006)]]. 10.0 ng/ul F21H11 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5211 Species: Caenorhabditis elegans Genotype: sEx314. Description: sEx314 [F22B7 (III) + pCes1943[rol-6(su1006)]]. 10.0 ng/ul F22B7 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5224 Species: Caenorhabditis elegans Genotype: sEx321. Description: sEx321 [D1022 (II) + pCes1943[rol-6(su1006)]]. 20 ng/ul D1022 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5229 Species: Caenorhabditis elegans Genotype: sEx326. Description: sEx326 [C28F5 (II) + pCes1943[rol-6(su1006)]]. 20 ng/ul C28F5 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5236 Species: Caenorhabditis elegans Genotype: sEx333. Description: sEx333 [C30G12 (II) + pCes1943[rol-6(su1006)]]. 6.4 ng/ul C30G12 + 100 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5237 Species: Caenorhabditis elegans Genotype: sEx334. Description: sEx334 [C08C3 (III) + pCes1943[rol-6(su1006)]]. 10 ng/ul C08C3 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5243 Species: Caenorhabditis elegans Genotype: sEx340. Description: sEx340 [F11D12 (III) + pCes1943[rol-6(su1006)]]. 10 ng/ul F11D12 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5256 Species: Caenorhabditis elegans Genotype: sEx351. Description: sEx351 [F43C1 (III) + pCes1943[rol-6(su1006)]]. 20 ng/ul F43C1 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5258 Species: Caenorhabditis elegans Genotype: sEx343. Description: sEx343 [T07F8 (II) + pCes1943[rol-6(su1006)]]. 20 ng/ul T07F8 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5267 Species: Caenorhabditis elegans Genotype: sEx345. Description: sEx345 [F13H10 (IV) + pCes1943[rol-6(su1006)]]. 20 ng/ul F13H10 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5273 Species: Caenorhabditis elegans Genotype: sEx352. Description: sEx352 [T05E11 (IV) + pCes1943[rol-6(su1006)]]. 20 ng/ul T05E11 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5276 Species: Caenorhabditis elegans Genotype: sEx355. Description: sEx355 [F01G4 (IV) + pCes1943[rol-6(su1006)]]. segrgnt. A. 20 ng/ul F01G4 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 04/15/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5280 Species: Caenorhabditis elegans Genotype: sEx359. Description: sEx359 [F36H1 (IV) + pCes1943[rol-6(su1006)]]. 20 ng/ul F36H1 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5287 Species: Caenorhabditis elegans Genotype: sEx362. Description: sEx362 [B0284 (III) + pCes1943[rol-6(su1006)]]. 20 ng/ul B0284 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5299 Species: Caenorhabditis elegans Genotype: sEx374. Description: sEx374 [F17C8 (III) + pCes1943[rol-6(su1006)]]. 20 ng/ul F17C8 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5301 Species: Caenorhabditis elegans Genotype: sEx376. Description: sEx376 [ZK1290 (II) + pCes1943[rol-6(su1006)]]. 10 ng/ul ZK1290 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: The Ha Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5302 Species: Caenorhabditis elegans Genotype: sEx377. Description: sEx377 [F43E2 (II) + pCes1943[rol-6(su1006)]]. 20 ng/ul F43E2 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5303 Species: Caenorhabditis elegans Genotype: sEx378. Description: sEx378 [C06A8 (II) + pCes1943[rol-6(su1006)]]. 20 ng/ul C06A8 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5305 Species: Caenorhabditis elegans Genotype: sEx380. Description: sEx380 [F56F3 (III) + pCes1943[rol-6(su1006)]]. 20 ng/ul F56F3 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5308 Species: Caenorhabditis elegans Genotype: sEx383. Description: sEx383 [R10E4 (III) + pCes1943[rol-6(su1006)]]. 20 ng/ul R10E4 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5311 Species: Caenorhabditis elegans Genotype: sEx386. Description: sEx386 [F10G11 (III) + pCes1943[rol-6(su1006)]]. 20 ng/ul F10G11 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5323 Species: Caenorhabditis elegans Genotype: sEx398. Description: sEx398 [R07E5 (III) + pCes1943[rol-6(su1006)]]. A small amount of R07E5 + 100 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5326 Species: Caenorhabditis elegans Genotype: sEx401. Description: sEx401 [B0495 (II) + pCes1943[rol-6(su1006)]]. 20 ng/ul B0495 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5334 Species: Caenorhabditis elegans Genotype: sEx409. Description: sEx409 [C16C10 (III) + pCes1943[rol-6(su1006)]]. 20 ng/ul C16C10 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5336 Species: Caenorhabditis elegans Genotype: sEx411. Description: sEx411 [T23F11 (III) + pCes1943[rol-6(su1006)]]. 20 ng/ul T23F11 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: The Ha Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5343 Species: Caenorhabditis elegans Genotype: sEx782. Description: sEx782 [K09H9 (I) + pCes1943[rol-6(su1006)]]. 20 ng/ul K09H9 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 04/15/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5344 Species: Caenorhabditis elegans Genotype: sEx414. Description: sEx414 [C27A12 (I) + pCes1943[rol-6(su1006)]]. 2.8 ng/ul C27A12 + 100 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5352 Species: Caenorhabditis elegans Genotype: sEx422. Description: sEx422 [C05C12 (IV) + pCes1943[rol-6(su1006)]]. A low concentration of C05C12 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 01/13/99 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5358 Species: Caenorhabditis elegans Genotype: sEx428. Description: sEx428 [T22B3 (IV) + pCes1943[rol-6(su1006)]]. 20 ng/ul T22B3 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5363 Species: Caenorhabditis elegans Genotype: sEx433. Description: sEx433 [ZK593 (IV) + pCes1943[rol-6(su1006)]]. segrgnt B. 20 ng/ul ZK593 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 04/15/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5364 Species: Caenorhabditis elegans Genotype: sEx434. Description: sEx434 [K01A6 (IV) + pCes1943[rol-6(su1006)]]. 20 ng/ul K01A6 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 04/15/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5367 Species: Caenorhabditis elegans Genotype: sEx437. Description: sEx437 [ZK596 (IV) + pCes1943[rol-6(su1006)]]. 20 ng/ul ZK596 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5370 Species: Caenorhabditis elegans Genotype: sEx440. Description: sEx440 [K02F2 (I) + pCes1943[rol-6(su1006)]]. 20 ng/ul K02F2 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5371 Species: Caenorhabditis elegans Genotype: sEx441. Description: sEx441 [F13E9 (IV) + pCes1943[rol-6(su1006)]]. 20 ng/ul F13E9 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5373 Species: Caenorhabditis elegans Genotype: sEx443. Description: sEx443 [T11G6 (IV) + pCes1943[rol-6(su1006)]]. 20 ng/ul T11G6 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5377 Species: Caenorhabditis elegans Genotype: sEx447. Description: sEx447 [K08F4 (IV) + pCes1943[rol-6(su1006)]]. 20 ng/ul K08F4 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5405 Species: Caenorhabditis elegans Genotype: sEx788. Description: sEx788 [C03C11 (I) + pCes1943[rol-6(su1006)]]. line 2. "Low" amount of C03C11 + 100 ng/ul pCes1943[rol-6(su1006)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: The Ha Received: 05/26/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5411 Species: Caenorhabditis elegans Genotype: sEx470. Description: sEx470 [F37A4 (III) + pCes1943[rol-6(su1006)]]. 6 ng/ul F37A4 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5429 Species: Caenorhabditis elegans Genotype: sEx480. Description: sEx480 [W10G11 (II) + pCes1943[rol-6(su1006)]]. 20 ng/ul W10G11 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Janke Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5437 Species: Caenorhabditis elegans Genotype: sEx488. Description: sEx488 [T06F6 (III) + pCes1943[rol-6(su1006)]]. 6 ng/ul T06F6 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5453 Species: Caenorhabditis elegans Genotype: sEx504. Description: sEx504 [T01D10 (III) + pCes1943[rol-6(su1006)]]. segrgnt 1. 5 ng/ul T01D10 + 95 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: The Ha Received: 04/15/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5477 Species: Caenorhabditis elegans Genotype: sEx528. Description: sEx528 [F25H8 (IV) + pCes1943[rol-6(su1006)]]. 20 ng/ul F52H8 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5480 Species: Caenorhabditis elegans Genotype: sEx531. Description: sEx531 [K01H12 (IV) + pCes1943[rol-6(su1006)]]. "low" ng/ul K01H12 + 83 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5482 Species: Caenorhabditis elegans Genotype: sEx533. Description: sEx533 [ZK1251 (IV) + pCes1943[rol-6(su1006)]]. "low" ng/ul ZK1251 + 83 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5489 Species: Caenorhabditis elegans Genotype: sEx540. Description: sEx540 [C28H8 (III) + pCes1943[rol-6(su1006)]]. segrgnt A. "Low" amount of cosmid C28H8 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 04/15/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5494 Species: Caenorhabditis elegans Genotype: sEx545. Description: sEx545 [F27C8 (IV) + pCes1943[rol-6(su1006)]]. "low" ng/ul F27C8 + 83 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5496 Species: Caenorhabditis elegans Genotype: sEx547. Description: sEx547 [C33A12 (IV) + pCes1943[rol-6(su1006)]]. "low" ng/ul C33A12 + 83 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5508 Species: Caenorhabditis elegans Genotype: sEx558. Description: sEx558 [B0280 (III) + pCes1943[rol-6(su1006)]]. "low" ng/ul B0280 + 100 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5509 Species: Caenorhabditis elegans Genotype: sEx559. Description: sEx559 [C37D8 (III) + pCes1943[rol-6(su1006)]]. 20 ng/ul C37D8 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5513 Species: Caenorhabditis elegans Genotype: sEx563. Description: sEx563 [F31E3 (III) + pCes1943[rol-6(su1006)]]. 10 ng/ul F31E3 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5523 Species: Caenorhabditis elegans Genotype: sEx570. Description: sEx570 [C14A1 (X) + pCes1943[rol-6(su1006)]]. 20 ng/ul C14A1 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5525 Species: Caenorhabditis elegans Genotype: sEx572. Description: sEx572 [ZK1074 (X) + pCes1943[rol-6(su1006)]]. 20 ng/ul ZK1074 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 02/17/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5532 Species: Caenorhabditis elegans Genotype: sEx579. Description: sEx579 [R02E12 (X) + pCes1943[rol-6(su1006)]]. 10 ng/ul R02E12 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5542 Species: Caenorhabditis elegans Genotype: sEx794. Description: sEx794 [C14E2 (X) + pCes1943[rol-6(su1006)]]. 10 ng/ul C14E2 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 05/26/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5544 Species: Caenorhabditis elegans Genotype: sEx591. Description: sEx591 [K03E5 (I) + pCes1943[rol-6(su1006)]]. seg 2. 20 ng/ul K03E5 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 04/15/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5545 Species: Caenorhabditis elegans Genotype: sEx592. Description: sEx592 [D1054 (V) + pCes1943[rol-6(su1006)]]. Segrgnt. II. low ng/ul D1054 + 100 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 04/15/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5577 Species: Caenorhabditis elegans Genotype: sEx623. Description: sEx623 [C50C12 (II) + pCes1943[rol-6(su1006)]]. 20 ng/ul C50C12 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5600 Species: Caenorhabditis elegans Genotype: sEx630. Description: sEx630 [F52C9 (III) + pCes1943[rol-6(su1006)]]. 9.8 ng/ul F52C9 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5601 Species: Caenorhabditis elegans Genotype: sEx631. Description: sEx631 [D1044 (III) + pCes1943[rol-6(su1006)]]. line 2. 12.3 ng/ul D1044 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: The Ha Received: 04/15/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5603 Species: Caenorhabditis elegans Genotype: sEx633. Description: sEx633 [F40H6 (III) + pCes1943[rol-6(su1006)]]. line 3. 8.2 ng/ul F40H6 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: The Ha Received: 04/15/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5606 Species: Caenorhabditis elegans Genotype: sEx636. Description: sEx636 [F48E8 (III) + pCes1943[rol-6(su1006)]]. line 1. 9.8 ng/ul F48E8 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: The Ha Received: 04/15/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5612 Species: Caenorhabditis elegans Genotype: sEx642. Description: sEx642 [F19F10 (V) + pCes1943[rol-6(su1006)]]. 20 ng/ul F19F10 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5618 Species: Caenorhabditis elegans Genotype: sEx648. Description: sEx648 [F47D12 (III) + pCes1943[rol-6(su1006)]]. 8.2 ng/ul F47D12 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5653 Species: Caenorhabditis elegans Genotype: sEx669. Description: sEx669 [C10C5 (IV) + pCes1943[rol-6(su1006)]]. "low" ng/ul C10C5 + 83 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5657 Species: Caenorhabditis elegans Genotype: sEx673. Description: sEx673 [F49C12 (IV) + pCes1943[rol-6(su1006)]]. 20 ng/ul F49C12 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5658 Species: Caenorhabditis elegans Genotype: sEx674. Description: sEx674 [C56G2 (III) + pCes1943[rol-6(su1006)]]. 10 ng/ul C56G2 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5664 Species: Caenorhabditis elegans Genotype: sEx680. Description: sEx680 [F40F11 (IV) + pCes1943[rol-6(su1006)]]. 20 ng/ul F40F11 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5667 Species: Caenorhabditis elegans Genotype: sEx683. Description: sEx683 [C08F8 (IV) + pCes1943[rol-6(su1006)]]. 20 ng/ul C08F8 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5668 Species: Caenorhabditis elegans Genotype: sEx684. Description: sEx684 [K11E8 (IV) + pCes1943[rol-6(su1006)]]. Segrgnt. B. 20 ng/ul K11E8 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Jacquie Schein Received: 04/15/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5678 Species: Caenorhabditis elegans Genotype: sEx691. Description: sEx691 [T12F5 (I) + pCes1943[rol-6(su1006)]]. 20 ng/ul T12F5 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5684 Species: Caenorhabditis elegans Genotype: sEx697. Description: sEx697 [B0467 (I) + pCes1943[rol-6(su1006)]]. 10 ng/ul B0467 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5694 Species: Caenorhabditis elegans Genotype: sEx705. Description: sEx705 [C33D8 (III) + pCes1943[rol-6(su1006)]]. 5 ng/ul C33D8 + 95 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5700 Species: Caenorhabditis elegans Genotype: sEx714. Description: sEx714 [F57E5 (X) + pCes1943[rol-6(su1006)]]. 21 ng/ul F57E5 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5705 Species: Caenorhabditis elegans Genotype: sEx719. Description: sEx719 [F20H11 (III) + pCes1943[rol-6(su1006)]]. segrgnt 2. 15 ng/ul F20H11 + 85 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 09/16/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5710 Species: Caenorhabditis elegans Genotype: sEx724. Description: sEx724 [C34C12 (III) + pCes1943[rol-6(su1006)]]. 20 ng/ul C34C12 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5712 Species: Caenorhabditis elegans Genotype: sEx726. Description: sEx726 [F49E7 (X) + pCes1943[rol-6(su1006)]]. 20 ng/ul F49E7 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5713 Species: Caenorhabditis elegans Genotype: sEx727. Description: sEx727 [F54D1 (IV) + pCes1943[rol-6(su1006)]]. ? ng/ul F54D1 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5717 Species: Caenorhabditis elegans Genotype: sEx731. Description: sEx731 [T19E10 (II) + pCes1943[rol-6(su1006)]]. 20 ng/ul T19E10 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5722 Species: Caenorhabditis elegans Genotype: sEx735. Description: sEx735 [W05F2 (I) + pCes1943[rol-6(su1006)]]. Segrgnt. 1. 20 ng/ul W05F2 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Lynnette Kuervers Received: 04/15/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5725 Species: Caenorhabditis elegans Genotype: sEx738. Description: sEx738 [M01B12 (I) + pCes1943[rol-6(su1006)]]. Segrgnt. 3. 20 ng/ul M01B12 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 04/15/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5726 Species: Caenorhabditis elegans Genotype: sEx739. Description: sEx739 [C07A9 (III) + pCes1943[rol-6(su1006)]]. 12.5 ng/ul C07A9 + 87.5 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5732 Species: Caenorhabditis elegans Genotype: sEx744. Description: sEx744 [C48B6 (I) + pCes1943[rol-6(su1006)]]. 7 ng/ul C48B6 + 93 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5733 Species: Caenorhabditis elegans Genotype: sEx745. Description: sEx745 [M01E11 (I) + pCes1943[rol-6(su1006)]]. Segrgnt I. 10 ng/ul M01E11 + 90 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Diana Collins Received: 04/15/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5734 Species: Caenorhabditis elegans Genotype: sEx746. Description: sEx746 [B0261 (I) + pCes1943[rol-6(su1006)]]. 28 ng/ul B0261 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: Received: 03/09/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5775 Species: Caenorhabditis elegans Genotype: sEx793. Description: sEx793 [B2044 III + pCes1943[rol-6(su1006)]]. line 8. 20 ng/ul B0244 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: David Baillie/The Ha Received: 09/16/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5776 Species: Caenorhabditis elegans Genotype: sEx796. Description: sEx796 [R04B5 (V) + pCes1943[rol-6(su1006)]]. line 4. Low amount of cosmid R04B5 + 100 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: The Ha Received: 05/26/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC5780 Species: Caenorhabditis elegans Genotype: sEx798. Description: sEx798 [K10D2 (III) + pCes1943[rol-6(su1006)]]. line 4. 20 ng/ul K10D2 + 80 ng/ul pCes1943[rol-6(su1006dm)] injected into hermaphrodite from BC842 (N2 male strain). pCes1943 carries rol-6(su1006) [an EcoRI insert from pRF4] and a kanamycin cassette. Select Rollers to maintain. Presence of cosmid confirmed by PCR. This transgenic strains were constructed in collaboration with the C. elegans Genome Sequencing Consortium Labs in St. Louis, MO and Hinxton, Cambridge, UK. Funding for the creation of this transgenic strain was provided by a grant to Ann Rose and David Baillie from the Canadian Genome Analysis and Technology Program (CGAT), and from a grant from NSERC (Canada) to David Baillie. If you use this strain in work which you publish, please acknowledge these grants. Mutagen: Outcrossed: x Made by: David Baillie/The Ha Received: 09/16/98 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC694 Species: Caenorhabditis elegans Genotype: sDf5/unc-15(e73) I. Description: Heterozygotes are WT and segregate more WT, Uncs and L1 lethals. Pick WT to maintain. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: EMS Outcrossed: x>1 Made by: Rose A Received: 02/01/82 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC700 Species: Caenorhabditis elegans Genotype: sDf4/bli-4(e937) dpy-14(e188) I. Description: Heterozygotes are semi-Dpy and segregate more semi-Dpy, Dpy(ts)Bli(late) and early larval lethals. Pick semi-Dpy to maintain. (Some reports indicate that sDf4 is not transferred through sperm.) This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Formaldehyde Outcrossed: x Made by: Rose A Received: 06/01/82 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC917 Species: Caenorhabditis elegans Genotype: let-61(s65) unc-22(s7)/+ + IV. Description: Heterozygotes are WT and segregate WT and Lethal Twitchers. Lethal late larval. Maintain by picking WT which throw Lethal Twitchers. Hets twitch in 1% Nicotine. Mutagen: EMS Outcrossed: x Made by: Rogalski T Received: 12/01/83 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC918 Species: Caenorhabditis elegans Genotype: let-63(s170) unc-22(s7)/+ + IV. Description: Heterozygotes are WT and segregate WT and Lethal Twitchers. Lethal mid larval. Maintain by picking WT which throw Lethal Twitchers. Hets twitch in 1% Nicotine. Mutagen: EMS Outcrossed: x Made by: Rogalski T Received: 12/01/83 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC933 Species: Caenorhabditis elegans Genotype: unc-22(s7) let-66(s176)/+ + IV. Description: Heterozygotes are WT and segregate WT and Lethal Twitchers. Lethal early larval. Heterozygotes twitch in 1% nicotine. Mutagen: Outcrossed: x Made by: Rogalski T Received: 12/01/83 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC934 Species: Caenorhabditis elegans Genotype: let-59(s175) unc-22(s7)/+ + IV. Description: Heterozygotes are WT and segregate WT and dead eggs. Hets twitch in 1% nicotine. Maintain by picking Twitchers in 1% Nicotine. Mutagen: Outcrossed: x Made by: Rogalski T Received: 12/01/83 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC954 Species: Caenorhabditis elegans Genotype: let-64(s216) unc-22(s7)/+ + IV. Description: Heterozygotes are WT (twitch in 1% nicotine) and segregate WT and thin, twitcher sterile adults. Pick twitchers in 1% nicotine to maintain. Mutagen: Outcrossed: x Made by: T. Rogalski Received: 08/01/84 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC96 Species: Caenorhabditis elegans Genotype: unc-22(s16) IV. Description: Twitcher Unc. Recessive. Heterozygotes twitch in 1% Nicotine. Mutagen: EMS Outcrossed: x Made by: Moerman D Received: 07/01/80 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC962 Species: Caenorhabditis elegans Genotype: mars-1(s254) unc-22(s7)/+ + IV. Description: Heterozygotes are WT and segregate WT and Lethal Twitchers. Lethal mid-larval. Heterozygotes twitch in 1% Nicotine. Maintain by picking Twitchers in 1% Nicotine. Mutagen: Outcrossed: x Made by: T. Rogalski Received: 12/01/83 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC964 Species: Caenorhabditis elegans Genotype: let-56(s46) unc-22(s7)/unc-5(e152) dpy-4(e1166) IV. Description: Heterozygotes are WT and segregate more WT, DpyUncs and Lethal Twitchers. Lethal late larval. Heterozygotes Twitch in 1% Nicotine. Pick WT to maintain. Mutagen: EMS Outcrossed: x Made by: Rogalski T Received: 03/01/82 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC965 Species: Caenorhabditis elegans Genotype: let-59(s49) unc-22(s7)/unc-5(e152) dpy-4(e1166) IV. Description: Heterozygotes are WT and segregate WT, DpyUnc and Lethal Twitchers. Lethal early larval. Hets twitch in 1% Nicotine. Pick WT to maintain. Mutagen: EMS Outcrossed: x Made by: Rogalski T Received: 03/01/82 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC966 Species: Caenorhabditis elegans Genotype: let-60(s59) unc-22(s7)/unc-5(e152) dpy-4(e1166) IV. Description: Heterozygotes are WT and segregate WT, DpyUnc, and Lethal Twitchers. Lethal mid-larval (leaky). Heterozygotes twitch in nicotine. Mutagen: Outcrossed: x Made by: Rogalski T Received: 03/01/82 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC986 Species: Caenorhabditis elegans Genotype: sDp3 (III;f); eT1 (III;V). Description: Throws Unc-36 and WT. Maintain by picking WT. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie. Mutagen: Gamma Rays Outcrossed: x Made by: R. Rosenbluth Received: 07/01/85 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BC987 Species: Caenorhabditis elegans Genotype: unc-22(s7) let-52(s42)/+ + IV. Description: Heterozygotes are WT and segregate WT and Lethal Twitchers. Lethal early larval. Hets twitch in 1% Nicotine. Pick WT to maintain. Mutagen: EMS Outcrossed: x Made by: T. Roglaski Received: 12/01/83 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: BCN2081 Species: Caenorhabditis elegans Genotype: crgSi2081 II; unc-119(ed3) III. Description: crgSi2081 [rpl-28p::PuroR + myo-2p::GFP + Cbr-unc-119(+)] II. Superficially wild-type. Puromycin resistant. ttTi5605 transposon in EG4322 has been replaced by a single copy insertion crgSi2081. Reference: Semple JI, et al., Nat Methods. 2010 Sep;7(9):725-7. Mutagen: Mos1 Outcrossed: x Made by: Jennifer Semple Received: 01/07/11 Centre de Regulacio Genomica, Barcelona, Spain ----------------------------------------------------------------------------- Strain: BCN9071 Species: Caenorhabditis elegans Genotype: vit-2(crg9070[vit-2::gfp]) X. Description: GFP knocked into C terminal of vit-2 gene by CRISPR/Cas9 using self-excising drug selection cassette method of Dickinson et al. (2015, Genetics). Strain is derived from BCN9070, which was outcrossed to remove a linked background mutation on LG X causing transgenic males to mate poorly. Reference: Perez MF, et al. Nature. 2017 Dec 7;552(7683):106-109. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x8 Made by: Marcos Francisco Perez Received: 12/10/18 Centre de Regulacio Genomica, Barcelona, Spain ----------------------------------------------------------------------------- Strain: BE1 Species: Caenorhabditis elegans Genotype: sqt-1(sc1) II. Description: Homozygotes are short, dumpyish in L4 and adult. Roller in L3. Hets Roll. M-MATING+POOR <1%WT Mutagen: EMS Outcrossed: x Made by: Cox J Received: 03/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: BE100 Species: Caenorhabditis elegans Genotype: sqt-1(sc100) II. Description: Pseudo WT. Mutagen: EMS Outcrossed: x2 Made by: R. Edgar Received: 10/01/85 University of California, Santa Cruz ----------------------------------------------------------------------------- Strain: BE101 Species: Caenorhabditis elegans Genotype: sqt-1(sc101) II. Description: Long, weak left-handed rollers in L3 and older. Mutagen: EMS Outcrossed: x2 Made by: R. Edgar Received: 10/01/85 University of California, Santa Cruz ----------------------------------------------------------------------------- Strain: BE102 Species: Caenorhabditis elegans Genotype: rol-8(sc102) II. Description: Weak left-handed roller as adult. Mutagen: EMS Outcrossed: x2 Made by: R. Edgar Received: 10/01/85 University of California, Santa Cruz ----------------------------------------------------------------------------- Strain: BE103 Species: Caenorhabditis elegans Genotype: sqt-1(sc103) II. Description: Pseudo WT. Mutagen: EMS Outcrossed: x2 Made by: R. Edgar Received: 10/01/85 University of California, Santa Cruz ----------------------------------------------------------------------------- Strain: BE104 Species: Caenorhabditis elegans Genotype: sqt-1(sc104) II. Description: Homozygotes are slightly Dpy. Heterozygotes are right roller. Mutagen: EMS Outcrossed: x2 Made by: R. Edgar Received: 10/01/85 University of California, Santa Cruz ----------------------------------------------------------------------------- Strain: BE107 Species: Caenorhabditis elegans Genotype: sqt-1(sc107) II. Description: Pseudo WT. Mutagen: EMS Outcrossed: x2 Made by: M. Kusch Received: 10/01/85 University of California, Santa Cruz ----------------------------------------------------------------------------- Strain: BE108 Species: Caenorhabditis elegans Genotype: sqt-2(sc108) II. Description: Homozygotes are squat and heterozygotes are right rollers. Mutagen: EMS Outcrossed: x3 Made by: M. Kusch Received: 10/01/85 University of California, Santa Cruz ----------------------------------------------------------------------------- Strain: BE109 Species: Caenorhabditis elegans Genotype: ?(sc109) V. Description: As homozygote suppresses blister formation in bli-1(sc73), bli-2(sc768) and bli-6(sc16). WT phenotype. Males sometimes have small blisters above their bursas. Males mate well. See wbg9.1p51. Mutagen: EMS Outcrossed: x>5 Made by: M. Kusch Received: 10/01/85 University of California, Santa Cruz ----------------------------------------------------------------------------- Strain: BE13 Species: Caenorhabditis elegans Genotype: sqt-1(sc13) II. Description: Left handed roller. Recessive. Mutagen: EMS Outcrossed: x Made by: Cox J Received: 03/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: BE148 Species: Caenorhabditis elegans Genotype: rol-9(sc148) V. Description: Strong right roller. Mutagen: EMS Outcrossed: x3 Made by: R. Russell Received: 05/26/87 University of California, Santa Cruz ----------------------------------------------------------------------------- Strain: BE149 Species: Caenorhabditis elegans Genotype: rol-9(sc149) V. Description: Strong right roller. Mutagen: EMS Outcrossed: x3 Made by: R. Russell Received: 05/26/87 University of California, Santa Cruz ----------------------------------------------------------------------------- Strain: BE15 Species: Caenorhabditis elegans Genotype: rol-8(sc15) II. Description: Left roller as adult. Mutagen: EMS Outcrossed: x2 Made by: Kusch and Cox Received: 10/01/85 University of California, Santa Cruz ----------------------------------------------------------------------------- Strain: BE150 Species: Caenorhabditis elegans Genotype: unc-51(e369) rol-9(sc148) V. Description: Roller Unc. Mutagen: Outcrossed: x Made by: Sara Roberts Received: 05/26/87 University of California, Santa Cruz ----------------------------------------------------------------------------- Strain: BE16 Species: Caenorhabditis elegans Genotype: bli-6(sc16) IV. Description: Blistered cuticle. Recessive. Mutagen: EMS Outcrossed: x Made by: Ward S Received: 07/01/80 University of California, Santa Cruz ----------------------------------------------------------------------------- Strain: BE22 Species: Caenorhabditis elegans Genotype: rol-1(sc22) II. Description: Left hand Roller. Recessive. Temperature sensitive. Mutagen: EMS Outcrossed: x Made by: Cox J Received: 07/01/82 University of California, Santa Cruz ----------------------------------------------------------------------------- Strain: BE26 Species: Caenorhabditis elegans Genotype: dpy-3(sc26) X. Description: Left-handed Dpy Roller. Recessive. Mutagen: EMS Outcrossed: x Made by: Cox J Received: 07/01/82 University of California, Santa Cruz ----------------------------------------------------------------------------- Strain: BE27 Species: Caenorhabditis elegans Genotype: dpy-7(sc27) X. Description: Temperature sensitive. Left hand Roller and Dpy at 25C. Dpyish at 16C. Recessive. Mutagen: EMS Outcrossed: x Made by: Received: 07/01/82 University of California, Santa Cruz ----------------------------------------------------------------------------- Strain: BE3 Species: Caenorhabditis elegans Genotype: sqt-2(sc3) II. Description: Heterozygotes Roll. M-MATING+POOR <1%WT. Mutagen: EMS Outcrossed: x Made by: Cox J Received: 03/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: BE38 Species: Caenorhabditis elegans Genotype: dpy-2(sc38) II. Description: Temperature sensitive. Left hand Roller and Dpy at 25C. Dpyish at 16C. Recessive. Mutagen: EMS Outcrossed: x Made by: Received: 07/01/82 University of California, Santa Cruz ----------------------------------------------------------------------------- Strain: BE42 Species: Caenorhabditis elegans Genotype: sqt-3(sc42) V. Description: Mutagen: EMS Outcrossed: x Made by: Kusch and Cox Received: 10/01/85 University of California, Santa Cruz ----------------------------------------------------------------------------- Strain: BE44 Species: Caenorhabditis elegans Genotype: dpy-8(sc44) X. Description: Temperature sensitive. Left hand Roller and Dpy at 25C. Wild-type at 16C. Recessive. Mutagen: EMS Outcrossed: x Made by: Received: 07/01/82 University of California, Santa Cruz ----------------------------------------------------------------------------- Strain: BE63 Species: Caenorhabditis elegans Genotype: sqt-3(sc63) V. Description: Dominant. Heterozygotes are Rollers. Temperature sensitive. At 25C: homozygotes are Sqt; heterozygotes are rollers. AT 16C: homozygotes are WT (a few adults may roll weakly); heterozygotes are WT. M-MATING+LOW TEMP ONLY. Mutagen: EMS Outcrossed: x Made by: Edgar B Received: 03/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: BE8 Species: Caenorhabditis elegans Genotype: sqt-3(sc8) V. Description: Adults and L4 animals roll left. Recessive. M-MATING++ 1-10%WT. PKA rol-4. Mutagen: EMS Outcrossed: x Made by: Cox J Received: 03/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: BE93 Species: Caenorhabditis elegans Genotype: dpy-2(e8) II. Description: Dpy. Recessive. Mutagen: EMS Outcrossed: x Made by: Edgar B Received: 03/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: BE97 Species: Caenorhabditis elegans Genotype: sqt-1(sc97) II. Description: WT morphology. Mutagen: EMS Outcrossed: x2 Made by: R. Edgar Received: 10/01/85 University of California, Santa Cruz ----------------------------------------------------------------------------- Strain: BE98 Species: Caenorhabditis elegans Genotype: rol-8(sc98) II. Description: Adults left-handed rollers. Mutagen: EMS Outcrossed: x2 Made by: R. Edgar Received: 10/01/85 University of California, Santa Cruz ----------------------------------------------------------------------------- Strain: BE99 Species: Caenorhabditis elegans Genotype: sqt-1(sc99) II. Description: Pseudo WT. Mutagen: EMS Outcrossed: x2 Made by: R. Edgar Received: 10/01/85 University of California, Santa Cruz ----------------------------------------------------------------------------- Strain: BFF40 Species: C. elegans Genotype: mjIs134 II; meg-3(tm4259) meg-4(ax2026) X. Description: mjIs134 [mex-5p::GFP::(Gly)5Ala::his-58::tbb-2 3'UTR + Cbr-unc-119(+)] II. Germ granule defective. ~30% sterility, maintain by picking gravid adults with visible embryos. Nuclear GFP expression in the germline. Reference: Lev I, et al. Curr Biol. 2019 Sep 9;29(17):2880-2891.e4. PMID: 31378614 Mutagen: Outcrossed: x0 Made by: Itai Toker Received: 04/19/22 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: BFF48 Species: C. elegans Genotype: mjIs134 II; hrde-1(tm1200) III. Description: mjIs134 [mex-5p::GFP::(Gly)5Ala::his-58::tbb-2 3'UTR + Cbr-unc-119(+)] II. Maintain at 15C. Heritable RNAi deficient, Mortal germline (Mrt) at 25C. Expressing nuclear GFP in the germline. Reference: Lev I, et al. Curr Biol. 2019 Sep 9;29(17):2880-2891.e4. PMID: 31378614 Mutagen: Outcrossed: x0 Made by: Itai Toker Received: 04/19/22 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: BFF49 Species: C. elegans Genotype: mjIs134 II; hrde-1(tm1200) III; meg-3(tm4259);meg-4(ax2026) X. Description: mjIs134 [mex-5p::GFP::(Gly)5Ala::his-58::tbb-2 3'UTR + Cbr-unc-119(+)] II. Maintain at 15C. Heritable RNAi deficient, germ granule defective, high sterility. Expresses nuclear GFP in the germline. Reference: Lev I, et al. Curr Biol. 2019 Sep 9;29(17):2880-2891.e4. PMID: 31378614 Mutagen: Outcrossed: x0 Made by: Itai Toker Received: 04/19/22 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: BFF53 Species: C elegans Genotype: bqSi577 IV. Description: bqSi577 [myo-2p::GFP + unc-119(+)] IV. Expresses GFP in pharyngeal muscles. Single-copy insertion in the MosSCI locus cxTi10882 on chromosome IV. Obtained via the outcrossing of strain BN578 with N2. Reference: Toker IA, et al. Dev Cell. 2022 Feb 7;57(3):298-309.e9. PMID: 35134343. Mutagen: Outcrossed: x0 Made by: Itai Toker Received: 06/08/22 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: BFF57 Species: C. elegans Genotype: srd-1(eh1) II; bqSi577 IV; meg-3(tm4259) meg-4(ax2026) X. Description: bqSi577 [myo-2p::GFP + unc-119(+)] IV. Germline granules defective, ~30% sterility. Males fail to be attracted by hermaphrodite-secreted volatile sex pheromones. Express GFP in pharyngeal muscles. Reference: Toker IA, et al. Dev Cell. 2022 Feb 7;57(3):298-309.e9. PMID: 35134343 Mutagen: Outcrossed: x0 Made by: Itai Toker Received: 04/19/22 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: BFF68 Species: C. elegans Genotype: mjIs134 II; hrde-1(pig6[degron::ha::hrde-1]) III. Description: mjIs134 [mex-5p::GFP::(Gly)5Ala::his-58::tbb-2 3'UTR + Cbr-unc-119(+)] II. N-terminal Auxin-Inducible Degron and HA tag inserted into the endogenous hrde-1 locus. gfp fluorescence in germline (mex-5::gfp). Control strain for BFF69; does not carry TIR1 transgene necessary for the degradation. hrde-1(pig6) crRNA sequence: CAUAAUUUUGUCGAGCAAGU. Reference: Toker IA, et al. Dev Cell. 2022 Feb 7;57(3):298-309.e9. PMID: 35134343. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Olga Antonova Received: 06/23/21 Tel Aviv University, Tel Aviv, Israel ----------------------------------------------------------------------------- Strain: BFF69 Species: C. elegans Genotype: mjIs134 II; hrde-1(pig6[degron::ha::hrde-1]) III; ieSi38 IV. Description: mjIs134 [mex-5p::GFP::(Gly)5Ala::his-58::tbb-2 3'UTR + Cbr-unc-119(+)] II. ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. N-terminal Auxin-Inducible Degron and HA tag inserted into the endogenous hrde-1 locus. Germline-expressed TIR1 and germline GFP. Allows for the degradation of hrde-1 and heritable RNAi deficiency in the presence of auxin. hrde-1(pig6) crRNA sequence: CAUAAUUUUGUCGAGCAAGU. Reference: Toker IA, et al. Dev Cell. 2022 Feb 7;57(3):298-309.e9. PMID: 35134343. Mutagen: Outcrossed: x0 Made by: Olga antonova Received: 06/23/21 Tel Aviv University, Tel Aviv, Israel ----------------------------------------------------------------------------- Strain: BFF70 Species: C. elegans Genotype: bqSi577 IV; meg-3(tm4259) meg-4(ax2026) X. Description: bqSi577 [myo-2p::GFP + unc-119(+)] IV. Germ granule defective, ~30 sterility. Express GFP in pharyngeal muscles. Reference: Toker IA, et al. Dev Cell. 2022 Feb 7;57(3):298-309.e9. PMID: 35134343 Mutagen: Outcrossed: x0 Made by: Itai Toker Received: 04/19/22 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: BG99 Species: Caenorhabditis elegans Genotype: laf-1(q267)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, Sterile Dpys and larval lethals (laf-1 homozygotes). Maintain by picking WT. Mutagen: EMS Outcrossed: x6 Made by: Received: 07/24/97 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: BIGb0170 Species: Sphingobacterium sp. Genotype: Sphingobacterium sp. Description: Bacteria. CeMbio Collection. Natural isolate from a C. elegans population in rotting apple. LB, 20-26C. Sampled in: Orsay, France. More information about collection on the project's wiki: http://www.cembio.uni-kiel.de/. 16S rRNA primer: 27F/1492R. 16S rRNA sequence: TGCAGTCGGACGGGANCCGTCGGAGAGCTTGCTCGAAGACGGTGAGAGTGGCGCACGG GTGCGTAACGCGTGAGCAACCTACCTCTATCAGGGGGATAGCCTCTCGAAAGAGAGATTAAC ACCGCATAACATATCTGACCGGCATCGGTTNGNTATTAAATATTTATAGGATAGAGATGGGCTC GCGTGACATTAGCTAGTTGGTAGGGTAACGGCTTACCAAGGCGACGATGTCTAGGGGCTCT GAGAGGAGAATCCCCCACACTGGTACTGAGACACGGACCAGACTCCTACGGGAGGCAGCA GTAAGGAATATTGGTCAATGGGCGGAAGCCTGAACCAGCCATGCCGCGTGCAGGATGACTG CCCTATGGGTTGTAAACTGCTTTTGTCCAGGAATAAACCTTTCTACGTGTAGGAAGCTGAATG TACTGGAAGAATAAGGATCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGATCCG AGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTAGGCGGCCTATTAAGTCAGGGGTGAAA TACGGTGGCTCAACCATCGCAGTGCCTTTGATACTGATGGGCTTGAATCCATTTGAAGTGGG CGGAATAAGACAAGTAGCGGTGAAATGCATAGATATGTCTTAGAACTCCGATTGCGAAGGCAG CTCACTAAGCTGGTATTGACGCTGATGCACGAAAGCGTGGGGATCGAACAGGATTAGATACC CTGGTAGTCCACGCCCTAAACGATGATAACTCGATGTTGGCGATAGACAGCCAGCGTCCAA GCGAAAGCGTTAAGTTATCCACCTGGGGAGTACGCCCGCAAGGGTGAAACTCAAAGGAATT GACGGGGGCCCGCACAAGCGGAGGAGCATGTGGTTTAATTCGATGATACGCGAGGAACCTT ACCCGGGCTTGAAAGTTAGTGAAGAATGCAGAGACGCATTCGTCCTTCGGGACACGAAACT AGGTGCTGCATGGCTGTCGTCAGCTCGTGCCGTGAGGTGTTGGGTTAAGTCCCGCAACGA GCGCAACCCCTATGTTTAGTTGCCAGCATGTAATGGNGGGGACTCTAAACAGACTGCCTGT GCAAA Mutagen: Outcrossed: x0 Made by: Wild isolate Received: 07/17/19 Baylor College of Medicine ----------------------------------------------------------------------------- Strain: BIGb0172 Species: Comamonas piscis Genotype: Comamonas piscis Description: Bacteria. CeMbio Collection. Natural isolate from a C. elegans population in rotting apple. Sampled in: Orsay, France. LB, 20-26C. Slow Grower. More information about collection on the project's wiki: http://www.cembio.uni-kiel.de/. 16S rRNA primer: 27F/1492R. 16S rRNA sequence: TATAGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGAACGGTAACAGGTCTTCGGATGCTGACGAGTGGCGAACGGGTGAGTAATACATCGGAACGTGCCTAGTAGTGGGGGATAACTACTCGAAAGAGTAGCTAATACCGCATGAGATCTAAGGATGAAAGCAGGGGATCGCAAGACCTTGTGCTACTAGAGCGGCTGATGGCAGATTAGGTAGTTGGTGGGATAAAAGCTTACCAAGCCGACGATCTGTAGCTGGTCTGAGAGGACGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGATCCAGCAATGCCGCGTGTAGGATGAAGGCCCTCGGGTTGTAAACTACTTTTGTACGGAACGAAAAGACTCTTTCTAATAAAGAGGGTCCATGACGGTACCGTAAGAATAAGCACCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTATGTAAGACAGAGGTGAAATCCCCGGGCTCAACCTGGGAACTGCCTTTGTGACTGCATAGCTAGAGTACGGTAGAGGGGGATGGAATTCCGCGTGTAGCAGTGAAATGCGTAGATATGCGGAGGAACACCGATGGCGAAGGCAATCCCCTGGACCTGTACTGACGCTCATGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGTCAACTGGTTGTTGGGAATTAACTTTCTCAGTAACGAAGCTAACGCGTGAAGTTGACCGCCTGGGGAGTACGGCCGCAAGGTTGAAACTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGATGATGTGGTTTAATTCGATGCAACGCGAAAAACCTTACCCACCTTTGACATGTACGGAAGTGACCAGAGATGGACATGTGCTCGAAAGAGAACCGTAACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGCCATTAGTTGCTACATTTAGTTGGGCACTCTAATGGGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTTATAGGTGGGGCTACACACGTCATACAATGGCTGGTACAAAGGGTTGCCAACCCGCGAGGGGGAGCTAATCCCATAAAGCCAGTCGTAGTCCGGATCGCAGTCTGCAACTCGACTGCGTGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGTCACGGTGAATACGTTCCCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGCGGGTCTCGCCAGAAGTAGGTAGCCTAACCGCAAGGAGGGCGCTTACCACGGCGGGGTTCGTGACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT Mutagen: Outcrossed: x0 Made by: Wild isolate Received: 07/17/19 Baylor College of Medicine ----------------------------------------------------------------------------- Strain: BIGb0393 Species: Pantoea sp. Genotype: Pantoea sp. Description: Bacteria. CeMbio Collection. Natural isolate from a C. elegans natural habitat (rotting Petasites stem). LB, 20-26C. Sampled in: Ivry, France. More information about collection on the project's wiki: http://www.cembio.uni-kiel.de/. 16S rRNA primer: 27F/1492R. 16S rRNA sequence: TGGAGCTTGCTCCTTGGGTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCC GATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGT GGGGGACCTTCGGGCCTCACACCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGG GTAATGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGA ACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCG CAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCA GCGGGGAGGAAGGCGGTGAGGTTAATAACCTCACCGATTGACGTTACCCGCAGAAGAAGCA CCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGNAATTA CTGGGCGTAAAGCGCACGCCGGCGGTCTGTCAAGTCGGATGTGAATCCCCGGGCTTAAC CTGGGAACTGCATTCGAAACTGGCAGGCTAGAGTCTCGTAGAGGGGGGTAGAATTCCAGGT GTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGAC GAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTC CACGCCGTAAACGATGTCGACTTGGAGGTTGTTCCCTTGAGGAGTGGCTTCCGGAGCTAA CGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGG GGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTA CTCTTGACATCCAGCGAACTTAGCAGAGATGCCCTGGTGCCTTCGGGAACCCTGAGACAG GTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGC GCAACCCTTATCCTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGA TAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGAGTAGGGCTACACA CGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGAGAGCAAGCGGACCTCATAAAG TGCGTCGTAGTCCGGATCGGAGTCTGCAACTCGACTCCGTGAAGTCGGAATCGCTAGTAAT CGTAGATCAGAATGCTACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACC ATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAAC Mutagen: Outcrossed: x0 Made by: Wild isolate Received: 07/17/19 Baylor College of Medicine ----------------------------------------------------------------------------- Strain: BJ21 Species: Caenorhabditis elegans Genotype: scm-1(hd30) I; sph-1(ox278) IV; sng-1(ok234) X. Description: Mutagen: Outcrossed: x12 Made by: RE Leube Received: 08/28/06 RWTH Aachen University, Aachen, Germany ----------------------------------------------------------------------------- Strain: BJH728 Species: C. elegans Genotype: unc-26(pek288[R216Q]) IV. Description: unc-26(pek288) is a CRISPR-engineered R216Q substitution in unc-26/synaptojanin 1 associated with early-onset Parkinsonism (EOP) (Quadri et al., 2013; Krebs et al., 2013). This allele shows abnormal focal accumulation of ATG-9 in presynaptic nerve terminals, defects in activity-induced synaptic autophagy, and defects in sustained neurotransmission and locomotory behaviors in aging animals. Reference: Yang S, et al. Neuron. 2022 Mar 2;110(5):824-840.e10. PMID: 35065714 Mutagen: Outcrossed: x1 Made by: Sisi Yang Received: 08/24/22 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: BJS4 Species: Caenorhabditis elegans Genotype: xpg-1(sbj4) I. Description: G to A splice acceptor mutation, LG I. position 6561778. Reference: Wolters S, et al. Genetics. 2014 Apr;196(4):985-99. Mutagen: EMS Outcrossed: x8 Made by: Stefanie Wolters Received: 03/03/14 University of Cologne, Cologne, Germany ----------------------------------------------------------------------------- Strain: BJS737 Species: C. elegans Genotype: mpk-1(sbj10) III. Description: Temperature sensitive allele of mpk-1, bypasses UV sensitivity of csb-1 mutant at 20-25C. Reference: Bianco JN & Schumacher B. Nucleic Acids Res. 2018 May 21. doi: 10.1093/nar/gky404. Mutagen: EMS Outcrossed: x0 Made by: Julien N Bianco Received: 08/09/18 University of Cologne, Cologne, Germany ----------------------------------------------------------------------------- Strain: BJS78 Species: Caenorhabditis elegans Genotype: smc-5(sbj3)/mIn1 [mIs14 dpy-10(e128)] II. Description: Homozygous viable mutation balanced by GFP- and dpy-10-marked inversion. Heterozygotes are wild-type with pharyngeal GFP signal, and segregate wild-type GFP+, Dpy bright GFP+ (mIn1 homozygotes), and non-GFP sbj3 homozygotes. Pick wild-type GFP+ to maintain. sbj3 homozygotes are morphologically wild-type but show reduction in viable progeny. Reference: Wolters S, et al. Genetics. 2014 Apr;196(4):985-99. Mutagen: EMS Outcrossed: x8 Made by: Stefanie Wolters Received: 02/11/14 University of Cologne, Cologne, Germany ----------------------------------------------------------------------------- Strain: BJS79 Species: Caenorhabditis elegans Genotype: smc-5(sbj2)/mIn1 [mIs14 dpy-10(e128)] II. Description: Homozygous viable mutation balanced by GFP- and dpy-10-marked inversion. Heterozygotes are wild-type with pharyngeal GFP signal, and segregate wild-type GFP+, Dpy bright GFP+ (mIn1 homozygotes), and non-GFP sbj3 homozygotes. Pick wild-type GFP+ to maintain. sbj3 homozygotes are morphologically wild-type but show reduction in viable progeny. Reference: Wolters S, et al. Genetics. 2014 Apr;196(4):985-99. Mutagen: EMS Outcrossed: x8 Made by: Stefanie Wolters Received: 02/11/14 University of Cologne, Cologne, Germany ----------------------------------------------------------------------------- Strain: BK36 Species: Caenorhabditis elegans Genotype: qpIs11 I; unc-119(ed3) III. Description: qpIs11 [vha-1p::GFP + unc-119(+)] I. Strong expression of GFP in the cytoplasm of the excretory canal cell (exc) and slightly less strong expression in the head mesodermal cell (hmc). Reference: Mattingly BC & Buechner M. Dev Biol. 2011 Nov 1;359(1):59-72. Mutagen: Psoralen + UV Outcrossed: x>5 Made by: Brendan C. Mattingly Received: 03/15/25 University of Kansas, Lawrence, KS ----------------------------------------------------------------------------- Strain: BL5717 Species: Caenorhabditis elegans Genotype: inIs179 II; him-8(e1489) IV. Description: inIs179 [ida-1p::GFP]. GFP is expressed in a subset of neurons and the neuroendocrine uv1 cells of the vulva. Identified neurons include ADE, ALA, ASI, ASK, AUA, ASG, AVH, AVJ, AVK, VC, HSN, PDE, PVP, PHA, PHB, PHC. Male sex-specific neurons include CA and some ray neurons. Plasmid pida-1::GFP 7.6 was injected into N2 and integrated to generate BL5715, then crossed into him-8(e1489) background. Mutagen: gamma irradiation Outcrossed: x2 Made by: T Zahn/P MacMorris Received: 01/21/03 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: BL5752 Species: Caenorhabditis elegans Genotype: inIs181; inIs182. Description: inIs181 [ida-1p::GFP]. inIs182 [ida-1p::GFP]. This is a strain in which two integrated copies of the same construct were crossed into a single strain in order to have stronger expression. Both arrays contain pTZ3i, which is ida-1::GFP with 2.4 kb of ida-1 promoter driving expression of ida-1::GFP fusion proten with ida-1 introns 3-9 included. Mutagen: Outcrossed: x2 Made by: Tobias Zahn Received: 04/05/06 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: BN1023 Species: C. elegans Genotype: bqSi294 II; bqSi1021 IV. Description: bqSi294 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::his-58 + unc-119(+)] II; bqSi1021 [lin-31p::FLP::SL2::mNG + unc-119(+)] IV. Heat shock induces nuclear GFP expression in P1.p-P11.p and nuclear mCherry expression elsewhere. Constitutive expression of diffusible mNeonGreen in P1.p-P11.p can mask GFP::HIS-58 signal. May carry unc-119(ed3) or unc-119(ed9) III. Reference: Fragoso-Luna A, et al. 2021 bioRxiv 2021.12.21.473632; doi: https://doi.org/10.1101/2021.12.21.473632 Mutagen: none Outcrossed: x2 Made by: Cristina Ayuso Received: 03/28/22 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN1029 Species: C. elegans Genotype: bqSi294 II; bqSi1027 IV. Description: bqSi294 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::his-58 + unc-119(+)] II; bqSi1027 [unc-122p::FLP::SL2::mNG + unc-119(+)] IV. Heat shock induces nuclear GFP expression in coelomocytes and nuclear mCherry expression elsewhere. Constitutive expression of diffusible mNeonGreen in coelomocytes can mask GFP::HIS-58 signal. Might carry unc-119(ed3) or unc-119(ed9) III. Reference: Fragoso-Luna A, et al. 2021 bioRxiv 2021.12.21.473632; doi: https://doi.org/10.1101/2021.12.21.473632 Mutagen: none Outcrossed: x2 Made by: Cristina Ayuso Received: 03/28/22 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN1082 Species: C. elegans Genotype: npp-2(bq38 [g>f>p::npp-2]) I; bqSi189 II. Description: bqSi189 [lmn-1p::mCherry::his-58 + unc-119(+)] II. Cassette for GFP-labeling and FLP-mediated inactivation of endogenous npp-2 inserted by CRISPR/Cas9 after the npp-2 start codon. FRT sites in GFP introns 2 & 3 are indicated by ">" symbols in genotype. Ubiquitous expression of mCh::HIS-58. Might carry unc-119(ed3) III. Reference: Fragoso-Luna A, et al. 2021 bioRxiv 2021.12.21.473632; doi: https://doi.org/10.1101/2021.12.21.473632 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Cristina Ayuso Received: 03/28/22 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN1097 Species: C. elegans Genotype: bqSi1030 II; bqSi548 IV. Description: bqSi1030 [hsp16.41p::FRT::mCherry::his-58::FRT::vhhGFP4::zif-1 + unc-119(+)] II. bqSi548 [dpy-7p::FLP + unc-119(+)] IV. Can be used for inducible degradation of GFP fusion proteins in hypodermal cell. May carry unc-119(ed3) III. Reference: Fragoso-Luna A, et al. 2021 bioRxiv 2021.12.21.473632; doi: https://doi.org/10.1101/2021.12.21.473632 Mutagen: No mutagen used Outcrossed: x2 Made by: Cristina Ayuso Received: 03/28/22 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN1117 Species: C. elegans Genotype: bqSi1030 II; bqSi508 IV. Description: bqSi1030 [hsp16.41p::FRT::mCherry::his-58::FRT::vhhGFP4::zif-1 + unc-119(+)] II. bqSi508 [elt-2p::FLP + unc-119(+)] IV. Can be used for inducible degradation of GFP fusion proteins in intestinal cells. May carry unc-119(ed3) III. Reference: Fragoso-Luna A, et al. 2021 bioRxiv 2021.12.21.473632; doi: https://doi.org/10.1101/2021.12.21.473632 Mutagen: none Outcrossed: x2 Made by: Cristina Ayuso Received: 03/28/22 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN1204 Species: C. elegans Genotype: bqSi294 II; bqSi1201 IV. Description: bqSi294 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::his-58 + unc-119(+)] II. bqSi1201 [hlh-12p::FLP::SL2::mNG + unc-119(+)] IV. Heat shock induces nuclear GFP expression in the distal tip cells and nuclear mCherry expression elsewhere. Constitutive expression of diffusible mNeonGreen in the distal tip cells can mask GFP::HIS-58 signal. Might carry unc-119(ed3) or unc-119(ed9) III. Reference: Fragoso-Luna A, et al. 2021 bioRxiv 2021.12.21.473632; doi: https://doi.org/10.1101/2021.12.21.473632 Mutagen: none Outcrossed: x2 Made by: Cristina Ayuso Received: 03/28/22 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN1216 Species: C. elegans Genotype: baf-1(bq52[gf>p::baf-1>]) III; bqSi577 IV. Description: bqSi577 [myo-2p::GFP + unc-119(+)] IV. Cassette for GFP-labeling and FLP-mediated inactivation of endogenous baf-1 inserted by CRISPR/Cas9 after the baf-1 start codon. FRT sites in GFP intron 3 and baf-1 3'UTR are indicated by ">" symbols in genotype. Reference: Fragoso-Luna A, et al. 2021 bioRxiv 2021.12.21.473632; doi: https://doi.org/10.1101/2021.12.21.473632 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Cristina Ayuso Received: 03/28/22 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN1319 Species: C. elegans Genotype: bqSi294 II; bqSi1308 IV. Description: bqSi294 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::his-58 + unc-119(+)] II; bqSi1308 [hlh-12p::FLP::SL2::mTagBFP2 + unc-119(+)] IV. Heat shock induces nuclear GFP expression in the distal tip cells and nuclear mCherry expression elsewhere. Constitutive expression of diffusible mTagBFP2 in the distal tip cells. Might carry unc-119(ed3) or unc-119(ed9) III. Reference: Fragoso-Luna A, et al. 2021 bioRxiv 2021.12.21.473632; doi: https://doi.org/10.1101/2021.12.21.473632 Mutagen: none Outcrossed: x2 Made by: Cristina Ayuso Received: 03/28/22 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN147 Species: Caenorhabditis elegans Genotype: emr-1(gk119) I; bqSi142 II. Description: bqSi142 [emr-1p::emr-1::mCherry + unc-119(+)] II. Might contain unc-119(ed3) in the background. Single-copy emr-1::mCherry transgene under control of emr-1 regulatory sequences. emr-1(gk119) embryos arrest when lem-2 is depleted by RNAi; bqSi142 fully rescues this phenotype. Reference: Morales-Martínez A, Dobrzynska A, Askjaer P. J Cell Sci. 2015 Feb 4. Mutagen: Outcrossed: x Made by: Peter Askjaer Received: 03/31/15 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN195 Species: Caenorhabditis elegans Genotype: bqSi195 II. Description: bqSi195 [(pBN65) hsp-16.41p::Dam::Myc::lmn-1 + unc-119(+)] II. Strain for DamID mapping of chromatin associated with the nuclear lamina/LMN-1. Made by MosSCI using strain EG4322 for injection. Might still carry unc-119(ed9) III, which is rescued by bqSi195. Reference: González-Aguilera C, et al. Genome Biol. 2014 Feb 3;15(2):R21. Mutagen: MosSCI Outcrossed: x2 Made by: C Gonzalez-Aguilera Received: 07/17/14 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN196 Species: Caenorhabditis elegans Genotype: bqSi196 II. Description: bqSi196 [hsp-16.41p::GFP::Myc::Dam + unc-119(+)] II. Strain for DamID mapping of chromatin associated with the nuclear lamina/LMN-1. Made by MosSCI using strain EG4322 for injection. Might still carry unc-119(ed9) III, which is rescued by bqSi196. Reference: González-Aguilera C, et al. Genome Biol. 2014 Feb 3;15(2):R21. Mutagen: MosSCI Outcrossed: x2 Made by: C Gonzalez-Aguilera Received: 07/17/14 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN218 Species: Caenorhabditis elegans Genotype: bqSi218 II. Description: bqSi218 [hsp-16.41p::Dam::Myc::emr-1 + unc-119(+)] II. Strain for DamID mapping of chromatin associated with the nuclear lamina/LMN-1. Made by MosSCI using strain EG4322 for injection. Might still carry unc-119(ed9) III, which is rescued by bqSi218. Reference: González-Aguilera C, et al. Genome Biol. 2014 Feb 3;15(2):R21. Mutagen: MosSCI Outcrossed: x2 Made by: C Gonzalez-Aguilera Received: 07/17/14 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN224 Species: Caenorhabditis elegans Genotype: lem-2(tm1582) bqSi210 II. Description: bqSi210 [lem-2p::lem-2::GFP + unc-119(+)] II. Might contain unc-119(ed3) in the background. Single-copy lem-2::GFP transgene under control of lem-2 regulatory sequences. lem-2(tm1582) embryos arrest when emr-1 is depleted by RNAi; bqSi210 fully rescues this phenotype. Reference: Morales-Martínez A, Dobrzynska A, Askjaer P. J Cell Sci. 2015 Feb 4. Mutagen: Outcrossed: x6 Made by: Peter Askjaer Received: 03/31/15 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN228 Species: Caenorhabditis elegans Genotype: bqSi210 II; bqSi226 IV. Description: bqSi210 [lem-2p::lem-2::GFP + unc-119(+)] II. bqSi226 [emr-1p::emr-1::mCherry + unc-119(+)] IV. Might contain unc-119(ed3) in the background. Single-copy lem-2::GFP transgene under control of lem-2 regulatory sequences and emr-1::mCherry under control of emr-1 regulatory sequences. Both transgenes are ubiquitously expressed and able to rescue development of animals without expression of endogenous lem-2 and emr-1. Reference: Morales-Martínez A, Dobrzynska A, Askjaer P. J Cell Sci. 2015 Feb 4. Mutagen: Outcrossed: x Made by: Peter Askjaer Received: 03/31/15 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN243 Species: Caenorhabditis elegans Genotype: bqSi235 II; bqSi242 IV. Description: bqSi235 [emr-1p::emr-1::GFP + unc-119(+)] II. bqSi226 [lem-2p::lem-2::mCherry + unc-119(+)] IV. Might contain unc-119(ed3) in the background. Single-copy emr-1::GFP transgene under control of emr-1 regulatory sequences and lem-2::mCherry under control of lem-2 regulatory sequences. Both transgenes are ubiquitously expressed and able to rescue development of animals without expression of endogenous lem-2 and emr-1. Reference: Morales-Martínez A, Dobrzynska A, Askjaer P. J Cell Sci. 2015 Feb 4. Mutagen: Outcrossed: x Made by: Peter Askjaer Received: 03/31/15 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN3 Species: Caenorhabditis elegans Genotype: vrk-1(ok1181)/mIn1 [mIs14 dpy-10(e128)] II. Description: F28B12.3. Homozygous sterile deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP ok1181 homozygotes (sterile adult). Pick WT dim GFP and check for correct segregation of progeny to maintain. Klerkx et al, Dev Biol. 2009 335:12-21. Mutagen: Outcrossed: x6 Made by: Elke Klerkx Received: 12/01/09 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN30 Species: Caenorhabditis elegans Genotype: npp-15(ok1954) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT and GFP+. ok1954 homozygotes arrest as larvae. Pick GFP+ heterozygotes to maintain. qIs48 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter driving GFP in the intestine, and is homozygous lethal. Reference: Rodenas E, et al. Mol Biol Cell. 2012 Mar;23(5):930-44. Mutagen: Outcrossed: x6 Made by: Eduardo Rodenas Received: 03/30/12 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN358 Species: Caenorhabditis elegans Genotype: ima-2(ok256) I/hT2[bli-4(e937) let-?(q782) qIs48] (I;III). Description: Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP sterile homozygotes (produce only dead embryos). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Derived from strain XA3503. Reference: ima-2(ok256) is described in Askjaer et al., Mol Biol Cell. 2002 Dec;13(12):4355-70. Mutagen: Outcrossed: x8 Made by: Peter Askjaer Received: 10/27/14 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN359 Species: Caenorhabditis elegans Genotype: ima-2(ok256) I/hT2[bli-4(e937) let-?(q782) qIs48] (I;III); qaIs3502. Description: qaIs3502 [pie-1p::YFP::lmn-1 + pie-1p::CFP::H2B + unc-119(+)]. Germline expression of YFP::lmn-1. CFP::HIS is silenced in qaIs3502. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP sterile homozygotes (produce only dead embryos). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Derived from strains XA3502 and XA3503. Reference: ima-2(ok256) is described in Askjaer et al., Mol Biol Cell. 2002 Dec;13(12):4355-70. Mutagen: Outcrossed: x8 Made by: Peter Askjaer Received: 10/27/14 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN360 Species: Caenorhabditis elegans Genotype: ima-2(ok256) I/hT2[bli-4(e937) let-?(q782) qIs48] (I;III); qaIs3502; ojIs1. Description: ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. qaIs3502 [pie-1p::YFP::lmn-1 + pie-1p::CFP::H2B + unc-119(+)]. Germline expression of YFP::lmn-1. CFP::HIS is silenced in qaIs3502. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP sterile homozygotes (produce only dead embryos). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Derived from strains XA3502 and XA3503. Reference: ima-2(ok256) is described in Askjaer et al., Mol Biol Cell. 2002 Dec;13(12):4355-70. Mutagen: Outcrossed: x8 Made by: Peter Askjaer Received: 10/27/14 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN40 Species: Caenorhabditis elegans Genotype: npp-5(tm3039)/mIn1 [mIs14 dpy-10(e128)] II. Description: Homozygous deletion chromosome balanced by GFP- and dpy-10-marked inversion. tm3039 homozygotes are viable but produce progengy that are primarily Lva or Lvl. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP tm3039 homozygotes. Pick WT dim GFP and check for correct segregation of progeny to maintain. Reference: Rodenas E, et al. Mol Biol Cell. 2012 Mar;23(5):930-44. Mutagen: Outcrossed: x6 Made by: Eduardo Rodenas Received: 03/30/12 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN426 Species: Caenorhabditis elegans Genotype: mel-28(bq5[GFP::mel-28]) III. Description: Strain ubiquitously expresses GFP::MEL-28 from the endogenous mel-28 locus. Transgene was inserted into parental strain N2 using CRISPR/Cas9 and co-conversion of dpy-10. Outcrossed twice to N2 to remove dpy-10. Reference: Gomez-Saldivar G, et al. PLoS Genet. 2016 Jun 24;12(6):e1006131. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: C. Ayuso Garcia Received: 02/17/17 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN452 Species: Caenorhabditis elegans Genotype: bqSi189 II; mel-28(bq5[GFP::mel-28]) III. Description: bqSi189 [lmn-1p::mCherry::his-58 + unc-119(+)] II. Strain ubiquitously expresses GFP::MEL-28 from the endogenous mel-28 locus. Transgene was inserted into parental strain N2 using CRISPR/Cas9 and co-conversion of dpy-10. Outcrossed twice to N2 to remove dpy-10. bqSi189 is a single-copy MosSCI insertion into ttTi5605 derived from injection of pBN13. Derived from parental strains BN189 and BN426. Reference: Gomez-Saldivar G, et al. PLoS Genet. 2016 Jun 24;12(6):e1006131. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: C. Ayuso Garcia Received: 02/17/17 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN455 Species: Caenorhabditis elegans Genotype: bqSi294 II; bqSi453 IV. Description: bqSi294 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::his-58 + unc-119(+)] II; bqSi453 [nhr-82p::FLP_CAI_G5D + unc-119(+)] IV. Heat shock produces green nuclei in the seam cell lineage and red nuclei elsewhere. Might still carry unc-119(ed3) III in background. Mutagen: Outcrossed: x2 Made by: Celia Muñoz-Jiménez Received: 07/31/17 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN46 Species: Caenorhabditis elegans Genotype: npp-19(tm2886) II; bqIs7. Description: bqIs7 [pie-1p::LAP::npp-19 + unc-119(+)]; rescues Emb defects. Rodenas et al, Dev Biol. 2009 327:399-409. Mutagen: Outcrossed: x6 Made by: Peter Askjaer Received: 12/01/09 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN464 Species: Caenorhabditis elegans Genotype: bqSi189 II; mel-28(t1684) unc-32(e189)/qC1 dpy-19(e1259) glp-1(q339) III; ojIs1 Description: bqSi189 [lmn-1p::mCherry::his-58 + unc-119(+)] II. ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. Maintain at 25C to help prevent silencing of ojIs1. mel-28 heterozygotes are wild-type and segregate wild-type, DpySteriles, and Uncs which give only dead eggs. ojIs1 is likely integrated into LG V. bqSi189 is a single-copy MosSCI insertion into ttTi5605 derived from injection of pBN13. Derived from GE2622; this strain might still carry him-3(e1147) in the background. Reference: Gomez-Saldivar G, et al. PLoS Genet. 2016 Jun 24;12(6):e1006131. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: C. Ayuso Garcia Received: 02/17/17 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN477 Species: Caenorhabditis elegans Genotype: bqSi471 II; bqSi225 IV. Description: bqSi471 [hsp-16.41p::FRT::mCherry::his-58::FRT::peel-1 + unc-119(+)] II; bqSi225 [emr-1p::emr-1::mCherry + unc-119(+)] IV. Expression of emr-1p::emr-1::mCherry marker is visible, but faint. Suitable for spatiotemporal cell ablation by crossing with FLP-expressing strains. Mutagen: Outcrossed: x2 Made by: Agnieszka Dobrzynska Received: 07/31/17 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN498 Species: Caenorhabditis elegans Genotype: bqSi294 II; bqSi487 IV. Description: bqSi294 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::his-58 + unc-119(+)] II; bqSi487 [mec-7p::FLP D5 + unc-119(+)] IV. Heat shock produces green nuclei in mechanosensory neurons and red nuclei elsewhere. Mutagen: Outcrossed: x2 Made by: Celia Muñoz-Jiménez Received: 07/31/17 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN499 Species: Caenorhabditis elegans Genotype: bqSi294 II; bqSi488 IV. Description: bqSi294 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::his-58 + unc-119(+)] II; bqSi488 [tph-1p::FLP D5 + unc-119(+)] IV. Heat shock produces green nuclei in serotonin-producing neurons and red nuclei elsewhere. Mutagen: Outcrossed: x2 Made by: Cristina Ayuso Received: 07/31/17 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN502 Species: Caenorhabditis elegans Genotype: bqSi294 II; bqSi494 IV. Description: bqSi294 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::his-58 + unc-119(+)] II; bqSi494 [hlh-8p::FLP D5 + unc-119(+)] IV. Heat shock produces green nuclei in the M lineage and red nuclei elsewhere. Mutagen: Outcrossed: x2 Made by: Celia Muñoz-Jiménez Received: 05/28/17 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN503 Species: Caenorhabditis elegans Genotype: bqSi294 II; bqSi495 IV. Description: bqSi294 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::his-58 + unc-119(+)] II; bqSi495 [myo-3p::FLP::D5 + unc-119(+)] IV. Heat shock produces green nuclei in body wall muscles and red nuclei elsewhere. Mutagen: Outcrossed: x2 Made by: Cristina Ayuso Received: 05/28/17 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN507 Species: Caenorhabditis elegans Genotype: bqSi294 II; bqSi506 IV. Description: bqSi294 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::his-58 + unc-119(+)] II; bqSi506 [rgef-1p::FLP D5 + unc-119(+)] IV. Heat shock produces green nuclei in neurons and red nuclei elsewhere. Mutagen: Outcrossed: x2 Made by: Cristina Ayuso Received: 07/31/17 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN528 Species: Caenorhabditis elegans Genotype: bqSi294 II; bqSi508 IV. Description: bqSi294 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::his-58 + unc-119(+)] II; bqSi508 [elt-2p::FLP D5 + unc-119(+)] IV. Heat shock produces green nuclei in intestinal cells (and some cells of the somatic gonad) and red nuclei elsewhere. Mutagen: Outcrossed: x2 Made by: Cristina Ayuso Received: 05/28/17 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN53 Species: Caenorhabditis elegans Genotype: vrk-1(ok1181)/mIn1 [mIs14 dpy-10(e128)] II; vrIs13. Description: vrIs13 [vrk-1p::VRK-1:GFP:VRK3UTR + unc-119(+)]. F28B12.3. Homozygous sterile deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP ok1181 homozygotes (sterile adult). Pick WT dim GFP and check for correct segregation of progeny to maintain. Klerkx et al, Dev Biol. 2009 335:12-21. Mutagen: Outcrossed: x6 Made by: Elke Klerkx Received: 12/01/09 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN543 Species: Caenorhabditis elegans Genotype: bqSi294 II; bqSi541 IV. Description: bqSi294 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::his-58 + unc-119(+)] II; bqSi541 [myo-2p::FLP D5 + unc-119(+)] IV. Heat shock produces green nuclei in pharyngal muscles and red nuclei elsewhere. Mutagen: Outcrossed: x2 Made by: Celia Muñoz-Jiménez Received: 05/28/17 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN544 Species: Caenorhabditis elegans Genotype: bqSi294 II; bqSi542 IV. Description: bqSi294 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::his-58 + unc-119(+)] II; bqSi542 [unc-47p::FLP D5 + unc-119(+)] IV. Heat shock produces green nuclei in GABAergic motor neurons and red nuclei elsewhere. Mutagen: Outcrossed: x2 Made by: Cristina Ayuso Received: 05/28/17 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN550 Species: Caenorhabditis elegans Genotype: bqSi294 II; bqSi548 IV. Description: bqSi294 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::his-58 + unc-119(+)] II; bqSi548 [dpy-7p::FLP D5 +unc-119(+)] IV.Heat shock produces green nuclei in the hypodermal cell lineage and red nuclei elsewhere. Mutagen: Outcrossed: x2 Made by: Celia Muñoz-Jiménez Received: 07/31/17 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN558 Species: Caenorhabditis elegans Genotype: bqSi294 II; bqSi556 IV. Description: bqSi294 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::his-58 + unc-119(+)] II; bqSi556 [lag-2p::FLP D5 + unc-119(+)] IV. Heat shock produces green nuclei in lag-2 expressing cells and red nuclei elsewhere. Mutagen: Outcrossed: x2 Made by: Cristina Ayuso Received: 05/28/17 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN578 Species: Caenorhabditis elegans Genotype: bqSi189 II; bqSi577 IV. Description: bqSi189 [lmn-1p::mCherry::his-58 + unc-119(+)] II; bqSi577 [myo-2p::GFP + unc-119(+)] IV. Strain carrying visible markers in the MosSCI loci on chr II (ttTi5605) and chr IV (cxTi10882); facilitates crosses between MosSCI strains with non-visible insertions. Mutagen: Outcrossed: x2 Made by: Patricia de la Cruz Ruiz Received: 07/31/17 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN580 Species: Caenorhabditis elegans Genotype: baf-1(bq12[g>f>p::baf-1]) III. Description: GFP cassette for labeling and FLP-mediated inactivation of baf-1 inserted by CRISPR/Cas9 after the baf-1 start codon. ">" symbols in genotype indicate Frt sites in introns 2 and 3 of GFP. Mutagen: Outcrossed: x2 Made by: Cristina Ayuso Received: 07/31/17 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN581 Species: C. elegans Genotype: baf-1(bq13[mCherry::baf-1]) III. Description: mCherry tag inserted into endogenous baf-1 locus after the start codon using by CRISPR/Cas9 engineering. Reference: Fragoso-Luna A, et al. 2021 bioRxiv 2021.12.21.473632; doi: https://doi.org/10.1101/2021.12.21.473632 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Cristina Ayuso Received: 03/28/22 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN617 Species: Caenorhabditis elegans Genotype: bqSi294 II; bqSi614 IV. Description: bqSi294 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::his-58 + unc-119(+)] II; bqSi614 [dat-1p::FLP D5::SL2::mNG + unc-119(+)] IV. Heat shock produces green nuclei in dopaminergic neurons and red nuclei elsewhere. Constitutive expression of diffusible mNeonGreen in dopaminergic neurons can mask GFP::HIS-58 signal. Mutagen: Outcrossed: x2 Made by: Cristina Ayuso Received: 07/31/17 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN646 Species: Caenorhabditis elegans Genotype: bqSi640 II; bqSi470 IV. Description: bqSi640 [dpy-7p::FRT::mCherry::his-58::FRT::GFP::his-58 + unc-119(+)] II; bqSi470 [hsp-16.41p::FLAG::NLS::FLP D5 + unc-119(+)] IV. Expression of red and green histones in hypodermal lineage before and after heat shock, respectively. Mutagen: Outcrossed: x2 Made by: Peter Askjaer Received: 07/31/17 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN69 Species: Caenorhabditis elegans Genotype: npp-5(tm3039)/mIn1 [mIs14 dpy-10(e128)] II; bqIs51 ltIs37 IV. Description: bqIs51 [pie-1p::GFP::npp-5 + unc-119(+)] IV. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Expresses GFP::NPP-5 and mCherry in the germ line. Homozygous deletion chromosome balanced by GFP- and dpy-10-marked inversion. tm3039 homozygotes are viable but produce progengy that are primarily Lva or Lvl; bqIs51 transgene rescues the npp-5(tm3039) phenotype. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP tm3039 homozygotes. Pick WT with dim GFP in the pharynx and check for correct segregation of progeny to maintain. Reference: Rodenas E, et al. Mol Biol Cell. 2012 Mar;23(5):930-44. Mutagen: Outcrossed: x6 Made by: Eduardo Rodenas Received: 03/30/12 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN711 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; bqSi711 IV. Description: bqSi711 [mex-5p::FLP::SL2::mNG + unc-119(+)] IV. Constitutive co-expression of codon-optimised FLP and fluorescent mNeonGreen in the germ line. Reference: Macias-Leon J & Askjaer P. (2018): Efficient FLP-mediated germ-line recombination in C. elegans. Micropublication:biology. Dataset. https://doi.org/10.17912/W2G66S Mutagen: Outcrossed: x0 Made by: Javier Macías-León Received: 04/12/18 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN793 Species: C. elegans Genotype: bqSi189 II; mel-28(bq17 [g>f>p::mel-28]) III. Description: bqSi189 [lmn-1p::mCherry::his-58 + unc-119(+)] II. Cassette for GFP-labeling and FLP-mediated inactivation of endogenous mel-28 inserted by CRISPR/Cas9 after the mel-28 start codon. FRT sites in GFP introns 2 & 3 are indicated by ">" symbols in genotype. Ubiquitous expression of mCherry::HIS-58. Reference: Fragoso-Luna A, et al. 2021 bioRxiv 2021.12.21.473632; doi: https://doi.org/10.1101/2021.12.21.473632 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Cristina Ayuso Received: 03/28/22 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN85 Species: Caenorhabditis elegans Genotype: npp-5(ok1966)/mIn1 [mIs14 dpy-10(e128)] II. Description: Homozygous deletion chromosome balanced by GFP- and dpy-10-marked inversion. ok1966 homozygotes are viable but produce progengy that are primarily Lva or Lvl. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP tm3039 homozygotes. Pick WT dim GFP and check for correct segregation of progeny to maintain. Reference: Rodenas E, et al. Mol Biol Cell. 2012 Mar;23(5):930-44. Mutagen: Outcrossed: x6 Made by: Eduardo Rodenas Received: 03/30/12 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN854 Species: C. elegans Genotype: bqSi294 II; bqSi852 IV. Description: bqSi294 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::his-58 + unc-119(+)] II. bqSi852 [ckb-3p::FLP::SL2::mNG + unc-119(+)] IV. Heat shock induces nuclear GFP expression in the somatic gonad and nuclear mCherry expression elsewhere. Constitutive expression of diffusible mNeonGreen in the somatic gonad can mask GFP::HIS-58 signal. Might carry unc-119(ed3) or unc-119(ed9) III. Reference: Fragoso-Luna A, et al. 2021 bioRxiv 2021.12.21.473632; doi: https://doi.org/10.1101/2021.12.21.473632 Mutagen: none Outcrossed: x2 Made by: Cristina Ayuso Received: 03/28/22 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN908 Species: Caenorhabditis elegans Genotype: unc-119(ed9) III; bqSi908 IV. Description: bqSi908 [UAS::FLP::SL2::mNG + unc-119(+)] IV. Strain for inducible co-expression of codon-optimised FLP recombinase and fluorescent mNeonGreen by cGAL (GAL4 optimised for C. elegans). Reference: Ayuso C & Askjaer P. Spatiotemporal control of genome recombination through combined FLP-Frt and GAL4-UAS technologies. microPublication Biology. https://doi.org/10.17912/MICROPUB.BIOLOGY.000089. Mutagen: Outcrossed: x0 Made by: Cristina Ayuso Received: 02/20/19 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BN999 Species: C. elegans Genotype: bqSi294 II; bqSi997 IV. Description: bqSi294 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::his-58 + unc-119(+)] II; bqSi997 [nhx-2p::FLP::SL2::mNG + unc-119(+)] IV. Heat shock induces nuclear GFP expression in intestinal cells and nuclear mCherry expression elsewhere. Constitutive expression of diffusible mNeonGreen in intestinal cells can mask GFP::HIS-58 signal. Might carry unc-119(ed3) or unc-119(ed9) III. Reference: Fragoso-Luna A, et al. 2021 bioRxiv 2021.12.21.473632; doi: https://doi.org/10.1101/2021.12.21.473632 Mutagen: none Outcrossed: x2 Made by: Cristina Ayuso Received: 03/28/22 CABD, CSIC-Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: BOX163 Species: C. elegans Genotype: erm-1(mib9[erm-1[T544D]]) I. Description: Homozygous viable. Modification of endogenous erm-1 locus mimics ERM-1(T544) phosphorylation. Variant affects ERM-1 localization and dynamics. Reduced brood size, increased embryonic and larval lethality. Reference: Ramalho JJ, et al. Development. 2020 Jul 22;147(14):dev188011. PMID: 32586975 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Joao J. Ramalho Received: 03/26/21 Utrecht University ----------------------------------------------------------------------------- Strain: BOX165 Species: C. elegans Genotype: erm-1(mib10[erm-1[T544A]]) I. Description: Homozygous viable. Modification of endogenous erm-1 locus mimics non-phosphorylated ERM-1(T544). Variant affects ERM-1 localization and dynamics. Reduced brood size, increased embryonic and larval lethality. Reference: Ramalho JJ, et al. Development. 2020 Jul 22;147(14):dev188011. PMID: 32586975 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Joao J. Ramalho Received: 03/26/21 Utrecht University ----------------------------------------------------------------------------- Strain: BOX188 Species: Caenorhabditis elegans Genotype: maph-1.1(mib12[GFP::maph-1.1]) I. Description: Endogenous maph-1.1 locus tagged with GFP using CRISPR/Cas9. Animals are homozygous viable and express GFP::maph-1.1 ubiquitously. Reference: Waaijers S, et al. BMC Biol. 2016 Aug 9;14:66. doi: 10.1186/s12915-016-0286-x. PMID: 27506200 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: JJ Ramalho Received: 07/17/19 Utrecht University ----------------------------------------------------------------------------- Strain: BOX213 Species: C. elegans Genotype: erm-1(mib15[erm-1::eGFP]) I. Description: Endogenous erm-1 locus tagged with eGFP. Homozygous viable, partially functional endogenous erm-1 tag. erm-1::GFP animals have a reduced brood size and incomplete outgrowth of the excretory canal, but show no other developmental or morphological abnormalities. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Joao J. Ramalho Received: 03/26/21 Utrecht University ----------------------------------------------------------------------------- Strain: BOX215 Species: C. elegans Genotype: erm-1(mib16[erm-1[T544D]::GFP]) I. Description: Homozygous viable. Endogenous erm-1 locus tagged with eGFP and modified to mimic ERM-1(T544) phosphorylation. Variant affects ERM-1 localization and dynamics. Reduced brood size, increased embryonic and larval lethality. eGFP-tagged ERM-1 is not fully functional: animals have a reduced brood size and incomplete outgrowth of the excretory canal, but show no other developmental or morphological abnormalities. The penetrance of intestinal phenotypes is slightly higher than in untagged T544 mutants, presumably owing to a detrimental influence of the COOH-terminal GFP tag. Reference: Ramalho JJ, et al. Development. 2020 Jul 22;147(14):dev188011. PMID: 32586975 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Joao J. Ramalho Received: 03/26/21 Utrecht University ----------------------------------------------------------------------------- Strain: BOX218 Species: C. elegans Genotype: erm-1(mib19[erm-1[T544A]::GFP]) I. Description: Homozygous viable. Endogenous erm-1 locus tagged with eGFP and modified to mimic non-phosphorylated ERM-1(T544). Variant affects ERM-1 localization and dynamics. Reduced brood size, increased embryonic and larval lethality. eGFP-tagged ERM-1 is not fully functional: animals have a reduced brood size and incomplete outgrowth of the excretory canal, but show no other developmental or morphological abnormalities. The penetrance of intestinal phenotypes is slightly higher than in untagged T544 mutants, presumably owing to a detrimental influence of the COOH-terminal GFP tag. Reference: Ramalho JJ, et al. Development. 2020 Jul 22;147(14):dev188011. PMID: 32586975 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Joao J. Ramalho Received: 03/26/21 Utrecht University ----------------------------------------------------------------------------- Strain: BP172 Species: Caenorhabditis elegans Genotype: muIs65 II; hyEx21. Description: hyEx21 [hsp-16.2p::eff-1 + rol-6(su1006)]. muIs65 [ajm-1::GFP + dpy-20(+)]. AJM-1 marks epithelial junctions. Pick Rollers to maintain. Mutagen: Outcrossed: x Made by: Meital Suissa Received: 02/29/08 Technion Research and Development Foundation, Haifa, Israel ----------------------------------------------------------------------------- Strain: BP175 Species: Caenorhabditis elegans Genotype: eff-1(hy21) II; hyEx22. Description: hyEx22 [hsp16-2::eff-1 + ajm-1::GFP + rol-6(su1006)]. Temperature sensitive eff-1 mutation (worms are cell fusion-defective at 25C; cell fusion defects are less penetrant at 15C). AJM-1 marks epithelial junctions. Pick GFP+ Rollers to maintain. Mutagen: Outcrossed: x Made by: Meital Suissa Received: 02/29/08 Technion Research and Development Foundation, Haifa, Israel ----------------------------------------------------------------------------- Strain: BP176 Species: Caenorhabditis elegans Genotype: hyEx23. Description: hyEx23 [des-2p::eff-1 + des-2::GFP + rol-6(su1006)]. Over-expression of EFF-1 in the PVDs. Shows reduced branching at L4 to adult stages. Pick GFP+ Rollers to maintain. Reference: Oren-Suissa M, et al. Science. 2010 Jun 4;328(5983):1285-8. Mutagen: Outcrossed: x Made by: Meital Oren-Suissa Received: 12/07/10 Technion Research and Development Foundation, Haifa, Israel ----------------------------------------------------------------------------- Strain: BP221 Species: Caenorhabditis elegans Genotype: eff-1(hy21) II; hyEx23. Description: hyEx23 [des-2p::eff-1 + des-2::GFP + rol-6(su1006)]. Cell-specific expression of eff-1 in the PVDs. Partially rescues arborization Eff-1 phenotypes. Pick GFP+ Rollers to maintain. Reference: Oren-Suissa M, et al. Science. 2010 Jun 4;328(5983):1285-8. Mutagen: Outcrossed: x Made by: Meital Oren-Suissa Received: 12/07/10 Technion Research and Development Foundation, Haifa, Israel ----------------------------------------------------------------------------- Strain: BP328 Species: Caenorhabditis elegans Genotype: eff-1(ok1021) II; hmIs4. Description: hmIs4 [des-2::GFP + rol-6(su1006)]. Dpy Rollers. eff-1(ok1021) was outcrossed 6 times. PVD neurons are hyperbranched and have disorganized menorah structures. Shows reduced sensitivity to strong mechanical stimuli. eff-1(ok1021) is a null allele and has strong arborization and cell fusion defects. Pick rollers to maintain. Reference: Oren-Suissa M, et al. Science. 2010 Jun 4;328(5983):1285-8. Mutagen: Outcrossed: x Made by: Meital Oren-Suissa Received: 12/07/10 Technion Research and Development Foundation, Haifa, Israel ----------------------------------------------------------------------------- Strain: BP387 Species: Caenorhabditis elegans Genotype: hyEx173. Description: hyEx173 [hsp16-2::aff-1 + ajm-1::GFP + rol-6(su1006)]. Pick Rollers to maintain. Rollers are GFP+. Mutagen: Outcrossed: x2 Made by: A. Sapir Received: 09/18/07 Technion Research and Development Foundation, Haifa, Israel ----------------------------------------------------------------------------- Strain: BP395 Species: Caenorhabditis elegans Genotype: hyEx167. Description: hyEx167 [4.5kb aff-1p::GFP transcriptional fusion + rol-6(su1006)]. Shows cytoplasmic GFP expression in embryonic hyp5. In L1 hermaphrodite larva, aff-1::GFP is expressed in pharyngeal muscle 3 and 5, in sheath cells of chemosensory neurons, and in tail neurons. In L3 hermaphrodites, the transgene is expressed in the anchor cell; this expression proceeds in L4 along with expression in the utse cells, the seam cells and cells of vulval rings A and D. In adults, aff-1::GFP is expressed in the sheath cells of chemosensory neurons and in head interneurons, uterus toroids 2 and 4, in the vulva VulD, utse, and seam cells. Worms of hyEx167 are slightly Egl. Maintain by picking Rollers. Mutagen: Outcrossed: x0 Made by: A. Sapir Received: 08/20/07 Technion Research and Development Foundation, Haifa, Israel ----------------------------------------------------------------------------- Strain: BP431 Species: Caenorhabditis elegans Genotype: eff-1(hy21) II; hmIs4. Description: hmIs4 [des-2::GFP + rol-6(su1006)]. Rollers. Temperature sensitive. PVD neurons are hyperbranched and have disorganized menorah structures. eff-1 worms are also cell fusion-defective at 25 C. Cell fusion and arborization defects are less penetrant at 15 C. Grow at 15 C to maintain. Pick rollers to maintain. Reference: Oren-Suissa M, et al. Science. 2010 Jun 4;328(5983):1285-8. Mutagen: Outcrossed: x Made by: Meital Oren-Suissa Received: 12/07/10 Technion Research and Development Foundation, Haifa, Israel ----------------------------------------------------------------------------- Strain: BP450 Species: Caenorhabditis elegans Genotype: hyEx30. Description: hyEx30 [myo-2::GFP + des-2::GFP + pBluescript]. Maintain by picking GFP+. Reference: Oren-Suissa M, et al. Science. 2010 Jun 4;328(5983):1285-8. Mutagen: Outcrossed: x Made by: Meital Oren-Suissa Received: 12/07/10 Technion Research and Development Foundation, Haifa, Israel ----------------------------------------------------------------------------- Strain: BP600 Species: Caenorhabditis elegans Genotype: aff-1(tm2214) II. Description: A 1.2 kb deletion in aff-1 (C44B7.3) which introduce stop codon after alanine 47. AFF-1 is a type I membrane protein necessary and sufficient for specific cell fusion events during embryonic and larval development. Temperature sensitivity was not detected. At 20° the fusion of hyp5 in the embryo does not occur as well as anchor cell (AC) fusion, vulval cells fusion of the A and D rings and the terminal fusion between the seam cells late in L4. 6% L1 rod-like lethal and 0% embryonic lethal. Adults are completely Egl, and partially Unc, Pvl. In addition, only 2% of AC in the mutant worms undergo fusion. These animals give very low brood size (16 progeny per worm) and 3.4% of the worms are sterile. This strain gives very small brood size and hence grows slowly. Originally from Shohei Mitani, Tokyo Women's Medical College, Tokyo, Japan. Mutagen: UV/TMP Outcrossed: x10 Made by: Amir Sapir Received: 08/20/07 Technion Research and Development Foundation, Haifa, Israel ----------------------------------------------------------------------------- Strain: BP601 Species: Caenorhabditis elegans Genotype: aff-1(tm2214)/mIn1 [dpy-10(e128) mIs14] II. Description: Homozygous sterile mutation balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and few GFP- tm2214 homozygotes animals which give very small brood size and hence can only be slowly propagated (see BP600 for detailed description for homozygote tm2214 phenotypes). Pick WT dim GFP and check for correct segregation of progeny to maintain. Mutagen: UV/TMP Outcrossed: x10 Made by: Amir Sapir Received: 08/20/07 Technion Research and Development Foundation, Haifa, Israel ----------------------------------------------------------------------------- Strain: BP610 Species: Caenorhabditis elegans Genotype: eff-1(ok1021) aff-1(tm2214)/mIn1 [mIs14 dpy-10(e128)] II. Description: Heterozygotes are WT with relatively dim pharyngeal GFP signal. Segregates Dpy with bright GFP (mIn1 homozygotes). Segregates very few escapers that are non-GFP (ok1021 tm2214 homozygotes). Mutagen: UV/TMP Outcrossed: x3 Made by: A. Sapir Received: 09/18/07 Technion Research and Development Foundation, Haifa, Israel ----------------------------------------------------------------------------- Strain: BP75 Species: Caenorhabditis elegans Genotype: eff-1(hy21) II. Description: Temperature sensitive. Cell fusion-defective embryos, larvae and adults at 25C. Cell fusion defects are less penetrant at 15C. Egl, Unv, Pvl, Dpy and 2% Muv at 20C and 25C. Mutants have body morphological defects and bulged tails at all temperatures, male tails are leptoderan. Partial sterility of hermaphrodites: brood size is 48 at 25C. sd-4%. ME=0. ES=3. OA-1 (oj55: complete embryonic and partial post-embryonic epithelial fusion failure). Cloned: encodes a type-I membrane glycoprotein with a single TM domain. Mutagen: EMS Outcrossed: x12 Made by: Received: 07/18/02 Technion Research and Development Foundation, Haifa, Israel ----------------------------------------------------------------------------- Strain: BP76 Species: Caenorhabditis elegans Genotype: eff-1(hy21) II; jcIs1 IV. Description: jcIs1 [ajm-1::GFP + unc-29(+) + rol-6(su1006)] IV. Temperature sensitive. Cell fusion-defective embryos, larvae and adults at 25C. Cell fusion defects are less penetrant at 15C. Egl, Unv, Pvl, Dpy and 2% Muv at 20C and 25C. Mutants have body morphological defects and bulged tails at all temperature, male tails are leptoderan. Partial sterility of hermaphrodites: brood size is 48 at 25C. ME=0. Cloned: ORF C26D10.5 encodes a type-I membrane glycoprotein with a single TM domain. ajm-1 was formerly known as jam-1 (Junction Associated Protein) and "the gene encoding the antigen recognized by the monoclonal antibody MH27." jcIs1 consists of pJS191, C45D3 and pRF4. Reference: Mohler WA, et al. Curr Biol. 1998 Sep 24;8(19):1087-90. Mutagen: Outcrossed: x Made by: G. Shemer Received: 09/18/02 University of Connecticut, Farmington, CT ----------------------------------------------------------------------------- Strain: BQ1 Species: Caenorhabditis elegans Genotype: akt-1(mg306) V. Description: Hid. Mutagen: EMS Outcrossed: x6 Made by: Patrick Hu Received: 01/22/08 Vanderbilt University Medical Center, Nashville, TN ----------------------------------------------------------------------------- Strain: BQ2 Species: Caenorhabditis elegans Genotype: eak-3(mg344) III. Description: Weak Hid. Eak. Mutagen: EMS Outcrossed: x6 Made by: Patrick Hu Received: 01/22/08 Vanderbilt University Medical Center, Nashville, TN ----------------------------------------------------------------------------- Strain: BQ3 Species: Caenorhabditis elegans Genotype: eak-4(mg348) IV. Description: Weak Hid. Eak. Mutagen: EMS Outcrossed: x6 Made by: Patrick Hu Received: 01/22/08 Vanderbilt University Medical Center, Nashville, TN ----------------------------------------------------------------------------- Strain: BQ4 Species: Caenorhabditis elegans Genotype: sdf-9(mg337) V. Description: Weak Hid. Eak. Mutagen: EMS Outcrossed: x6 Made by: Patrick Hu Received: 01/22/08 Vanderbilt University Medical Center, Nashville, TN ----------------------------------------------------------------------------- Strain: BQ5 Species: Caenorhabditis elegans Genotype: eak-6(mg329) I. Description: Weak Hid. Eak. Mutagen: EMS Outcrossed: x6 Made by: Patrick Hu Received: 01/22/08 Vanderbilt University Medical Center, Nashville, TN ----------------------------------------------------------------------------- Strain: BQ6 Species: Caenorhabditis elegans Genotype: eak-7(mg338) IV. Description: Weak Hid. Eak. Mutagen: EMS Outcrossed: x6 Made by: Patrick Hu Received: 04/26/12 Vanderbilt University Medical Center, Nashville, TN ----------------------------------------------------------------------------- Strain: BR2742 Species: Caenorhabditis elegans Genotype: pept-1(lg601) X. Description: Slow postembryonic development. Reduced brood size. Previously known as pep-2. [NOTE: the genotype of this strain was previously incorrectly annotated as lg1601. The correct allele name is lg601.] Reference: Meissner B, et al. J Biol Chem. 2004 Aug 27;279(35):36739-45. Mutagen: UV/TMP Outcrossed: x10 Made by: Elegene Received: 07/01/05 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR2815 Species: Caenorhabditis elegans Genotype: nep-1(by159) II. Description: 1450 bp deletion. Deletion removes 245 bp of promoter, 1st exon and 2nd exon. Mutagen: EMS Outcrossed: x8 Made by: Received: 02/08/06 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR2823 Species: Caenorhabditis elegans Genotype: chn-1(by155) I. Description: 989bp deletion. Primers used: RB1537 (external left): CAAGCTTAATTTGCACACAATGCGTCAG; RB1540 (external right): AGAAGAGGCAAGAATGGAACTTGTGCG; RB1538 (internal left): AACAATTTCCGCTTATTTTCAGCGTTTG; RB1539 (internal right): CTGAACCATTGAAAGCTTTTCAGATC. Deletion breakpoints (T09B4 coordinates): 32756/33746 through 32757/33747. Mutagen: EMS Outcrossed: x8 Made by: Hoppe/Sperl Received: 05/23/07 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR2958 Species: Caenorhabditis elegans Genotype: ceh-16(lg16) III; jcIs1 IV; ngEx1. Description: jcIs1 [ajm-1::GFP + unc-29(+) + rol-6(su1006)] IV. ngEx1[ceh-16::GFP]. ngEx1 rescues ceh-16(lg16) lethality. ajm-1 was formerly known as jam-1 (Junction Associated Protein) and "the gene encoding the antigen recognized by the monoclonal antibody MH27." jcIs1 consists of pJS191, C45D3 and pRF4. Reference: Cassata et al. Development. 2005 Feb;132(4):739-49. Mutagen: UV/TMP Outcrossed: x6 Made by: Giuseppe Cassata Received: 02/14/06 IFOM, Milano, Italy ----------------------------------------------------------------------------- Strain: BR3417 Species: Caenorhabditis elegans Genotype: spr-5(by134) I. Description: Suppressor of Egl of sel-12. Mutagen: UV/TMP Outcrossed: x5 Made by: Agata Smialowska Received: 07/01/05 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR4006 Species: Caenorhabditis elegans Genotype: pink-1(tm1779) II; byEx655. Description: byEx655 [pink-1p::pink-1::GFP + myo-2p::mCherry + herring sperm DNA]. Pick mCherry+ animals to maintain. pink-1 translational GFP (C-terminal GFP) fusion generated by cloning the complete pink-1 genomic fragment (3191 bp) with a 6-kb upstream promoter region and into pPD117.01. Reference: Sämann J, et al. J Biol Chem. 2009 Jun 12;284(24):16482-91. Mutagen: Outcrossed: x0 Made by: Franziska Haug Received: 02/18/15 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR5082 Species: C.elegans Genotype: shc-1(ok198) I; zIs356 IV. Description: zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)] IV. Tumorous germline with degeneration of surrounding extracellular matrix & disrupted gonadal basement membrane. Reference: Qi W, et al. PLoS Genet. 2012;8(8):e1002836. doi: 10.1371/journal.pgen.1002836. Mutagen: Outcrossed: x0 Made by: Wenjing Qi Received: 05/04/22 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR5270 Species: Caenorhabditis elegans Genotype: byIs161. Description: byIs161 [rab-3p::F3(delta)K280 + myo-2p::mCherry]. Pan-neuronal over-expression of the F3 pro aggregation fragment of the human Tau protein with K280 deleted (line A). Integration site not mapped. Reference: Fatuouros C, et al. Hum Mol Genet. 2012 Aug 15;21(16):3587-603. Mutagen: Gamma radiation Outcrossed: x5 Made by: C. Fatouros Received: 12/16/14 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR5271 Species: Caenorhabditis elegans Genotype: byIs162. Description: byIs162 [rab-3p::F3(delta)K280 I277P I380P + myo-2p::mCherry]. Pan-neuronal over-expression of the F3 pro aggregation fragment of the human Tau protein with K280 deleted and two isoleucines to proline substitutions in the hexapeptide motifs of the repeat region (line A). These mutations abrogate the aggregation, making this strain an anti-aggregation control for BR5270. Reference: Fatuouros C, et al. Hum Mol Genet. 2012 Aug 15;21(16):3587-603. Mutagen: Gamma radiation Outcrossed: x5 Made by: C. Fatouros Received: 12/16/14 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR5486 Species: Caenorhabditis elegans Genotype: byIs162; bkIs10. Description: byIs162 [rab-3p::F3(delta)K280(I277P)(I308P) + myo-2p::mCherry]. bkIs10 [aex-3p::h4R1NtauV337M + myo-2p::GFP]. Strain co-expressing the F3(delta)K280 anti-aggregation Tau fragment (with two I to P substitutions) and full-length Tau in all neurons. Red and green fluorescence in the pharynx due to the co-injection markers. The F3/2P fragment is not able to nucleate aggregation of full length Tau. bkIs10 contains an integrated transgene encoding the 1N4R isoforms of human tau with the 337M FTDP-17 mutation. Expression is driven by the pan-neuronal promoter aex-3. Over-expression of this transgene results in a pronounced Unc phenotype. Reference: Fatouros C, et al. Hum Mol Genet. 2012 Aug 15;21(16):3587-603. Mutagen: Outcrossed: x Made by: Chronis Fatouros Received: 06/20/16 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR5514 Species: Caenorhabditis elegans Genotype: tax-2(p671) I; tax-4(p678) III. Description: Reference: Liu S, Schulze E, Baumeister R. PLoS One. 2012;7(3):e32360. Mutagen: Outcrossed: x0 Made by: Shu Liu Received: 02/11/16 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR5602 Species: Caenorhabditis elegans Genotype: tax-4(p678) III; byEx836. Description: byEx836 [odr-4p::tax-4::GFP + myo-2p::mCherry]. Pick mCherry+ animals to maintain. Rescues tax-4 null mutant. Expresses tax-4(+) in AWA, AWB, AWC, ADF, ASG, ASH, ASI, ASJ, ASK, ADL, PHA, and PHB sensory neurons, but not AFD sensory neurons. Reference: Liu S, Schulze E, Baumeister R. PLoS One. 2012;7(3):e32360. Mutagen: Outcrossed: x0 Made by: Shu Liu Received: 05/28/15 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR5706 Species: Caenorhabditis elegans Genotype: byIs193; bkIs10. Description: byIs193 [rab-3p::F3(delta)K280 + myo-2p::mCherry]. bkIs10 [aex-3p::hTau V337M + myo-2p::GFP]. Worms have severe locomotion defect and slow growth. Reference: Fatuouros C, et al. Hum Mol Genet. 2012 Aug 15;21(16):3587-603. Mutagen: Gamma radiation Outcrossed: x5 Made by: C. Fatouros Received: 12/16/14 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR5944 Species: Caenorhabditis elegans Genotype: byIs193. Description: byIs193 [rab-3p::F3(delta)K280 + myo-2p::mCherry]. F3 pro aggregation fragment is expressed at low levels (line B); hard to detect by western blot. Reference: Fatuouros C, et al. Hum Mol Genet. 2012 Aug 15;21(16):3587-603. Mutagen: Gamma radiation Outcrossed: x5 Made by: C. Fatouros Received: 12/16/14 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR6427 Species: Caenorhabditis elegans Genotype: byIs194; bkIs10. Description: byIs194 [rab-3p::F3(delta)K280 I277P I380P + myo-2p::mCherry]. bkIs10 [aex-3p::hTau V337M + myo-2p::GFP]. Tau anti-aggregation double transgenic line generated by crossing byIs194 x bkIs10. Normal locomotion. Reference: Fatuouros C, et al. Hum Mol Genet. 2012 Aug 15;21(16):3587-603. Mutagen: Gamma radiation Outcrossed: x Made by: C. Fatouros Received: 12/16/14 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR6516 Species: Caenorhabditis elegans Genotype: byIs194. Description: byIs194 [rab-3p::F3(delta)K280 I277P I380P + myo-2p::mCherry]. Pan-neuronal over-expression of the F3 pro aggregation fragment of the human Tau protein with K280 deleted and two isoleucines to proline substitutions in the hexapeptide motifs of the repeat region (line B). These mutations abrogate the aggregation, making this strain an anti-aggregation control for BR5270. Reference: Fatuouros C, et al. Hum Mol Genet. 2012 Aug 15;21(16):3587-603. Mutagen: Gamma radiation Outcrossed: x5 Made by: C. Fatouros Received: 12/16/14 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR6563 Species: Caenorhabditis elegans Genotype: byIs161; bkIs10. Description: byIs161 [rab-3p::F3(delta)K280 + myo-2p::mCherry]. bkIs10 [aex-3p::h4R1NtauV337M + myo-2p::GFP]. Strain co-expressing the F3(delta)K280 pro-aggregation Tau fragment and full-length Tau in all neurons. Worms have severe locomotion defect and slow growth. Red and green fluorescence in the pharynx due to the co-injection markers. bkIs10 contains an integrated transgene encoding the 1N4R isoforms of human tau with the 337M FTDP-17 mutation. Expression is driven by the pan-neuronal promoter aex-3. Over-expression of this transgene results in a pronounced Unc phenotype. Derived by additional outcrossing of BR5485. Reference: Fatouros C, et al. Hum Mol Genet. 2012 Aug 15;21(16):3587-603. Mutagen: Outcrossed: x10 Made by: Chronis Fatouros Received: 06/20/16 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR7205 Species: C.elegans Genotype: endu-2(by190[endu-2::eGFP]) X. Description: eGFP tag inserted into the endogenous endu-2 locus. Reference: Qi W, et al. (2020) A secreted endoribonuclease ENDU-2 from the soma protects germline immortality in C. elegans. BioRxiv. doi: 10.1101/2020.12.04.408260. Accepted by Nature Communications. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Lijiang Long Received: 05/04/22 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR7295 Species: C.elegans Genotype: endu-2(tm4977) X; byEx1375. Description: byEx1375 [endu-2p::endu-2::eGFP + myo-2p::mCherry]. Pick mCherry+ animals to maintain array. Transgene rescues mortal germline (Mrt) phenotype of endu-2(tm4977). Reference: Qi W, et al. (2020) A secreted endoribonuclease ENDU-2 from the soma protects germline immortality in C. elegans. BioRxiv. doi: 10.1101/2020.12.04.408260. Accepted by Nature Communications. Mutagen: Outcrossed: x0 Made by: Wenjing Qi Received: 05/04/22 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR7827 Species: C.elegans Genotype: endu-2(tm4977) X; byEx1551. Description: byEx1551 [vha-6p::endu-2::eGFP::3xFLAG + myo-2p::mCherry]. Pick mCherry+ animals to maintain array. Transgene provides intestinal rescue of endu-2(tm4977) that also rescues mortal germline (Mrt) phenotype. Reference: Qi W, et al. (2020) A secreted endoribonuclease ENDU-2 from the soma protects germline immortality in C. elegans. BioRxiv. doi: 10.1101/2020.12.04.408260. Accepted by Nature Communications. Mutagen: Outcrossed: x0 Made by: Wenjing Qi Received: 05/04/22 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR8551 Species: C.elegans Genotype: endu-2(tm4977) X; byEx1795. Description: byEx1795 [unc-119p::endu-2::eGFP::3xFlag + rol-6(su1006)]. Pick Rollers to maintain. Transgene provides neuronal rescue of endu-2(tm4977) that also rescues mortal germline (Mrt) phenotype. Reference: Qi W, et al. (2020) A secreted endoribonuclease ENDU-2 from the soma protects germline immortality in C. elegans. BioRxiv. doi: 10.1101/2020.12.04.408260. Accepted by Nature Communications. Mutagen: Outcrossed: x0 Made by: Wenjing Qi Received: 05/04/22 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR8611 Species: Caenorhabditis elegans Genotype: pqm-1(ok485) II; sodh-1(ok2799) V. Description: Reference: Heimbucher T, et al. Nat Commun. 2020 Oct 2;11(1):4627. doi: 10.1038/s41467-020-18369-w. PMID: 33009389. Mutagen: Outcrossed: x0 Made by: Julian Received: 10/24/24 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR8724 Species: Caenorhabditis elegans Genotype: pqm-1(ok485) II; pwIs23. Description: pwIs23 [vit-2::GFP]. Reference: Heimbucher T, et al. Nat Commun. 2020 Oct 2;11(1):4627. doi: 10.1038/s41467-020-18369-w. PMID: 33009389. Mutagen: Outcrossed: x0 Made by: Julian Hog Received: 10/24/24 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR8807 Species: Caenorhabditis elegans Genotype: pqm-1(ok485) II; daf-2(e1370) III; nIs590 V. Description: nIs590 [fat-7p::fat-7::GFP + lin15(+)] V. Temperature sensitive dauer constitutive. Maintain at 15C. 100% dauers at 25C. 15% dauer formation at 20C. Long-lived. Reference: Heimbucher T, et al. Nat Commun. 2020 Oct 2;11(1):4627. doi: 10.1038/s41467-020-18369-w. PMID: 33009389. Mutagen: Outcrossed: x0 Made by: Piyush Gupta Received: 10/24/24 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR8808 Species: Caenorhabditis elegans Genotype: daf-2(e1370) III; nIs590 V. Description: nIs590 [fat-7p::fat-7::GFP + lin15(+)] V. Temperature sensitive dauer constitutive. Maintain at 15C. 100% dauers at 25C. 15% dauer formation at 20C. Long-lived. Reference: Heimbucher T, et al. Nat Commun. 2020 Oct 2;11(1):4627. doi: 10.1038/s41467-020-18369-w. PMID: 33009389. Mutagen: Outcrossed: x0 Made by: Piyush Gupta Received: 10/24/24 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BR8809 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; muIs139. Description: muIs139 [dod-11p::RFP::NLS + rol-6(su1006)]. Dauer defective. Short lived. Reference: Heimbucher T, et al. Nat Commun. 2020 Oct 2;11(1):4627. doi: 10.1038/s41467-020-18369-w. PMID: 33009389. Mutagen: Outcrossed: x0 Made by: Piyush Gupta Received: 10/24/24 Albert-Ludwigs-University of Freiburg, Freiburg, Germany ----------------------------------------------------------------------------- Strain: BRC546 Species: C. elegans Genotype: antIs30 II; unc-119(ed9) III. Description: antIs30 [attP-f + Cbr-unc-119(+) + glh-2p::phiC31 + rol-6(partial) + myo-2p::GFP + attP-r] II. antIs30 was inserted into ttTi5605 on LG II using MosSCI. GFP expression in pharynx is very weak (as it is single copy) and is easiest to see during the L1-L3 stages. This strain contains a phiC31 docking site and can be used for precise single-copy integration of transgenes via recombination mediated cassette exchange. The docking site contains inverted phiC31-attP sites flanking phiC31 integrase expressed from the glh-2 germline promoter. Integration constructs need to have inverted phiC31-attB sites that flank the intended sequence to be inserted. Reference: Yang FJ, et al. "phiC31 integrase for recombination mediated single copy insertion and genome manipulation in C. elegans." Genetics 2021. Mutagen: MosSCI Outcrossed: x0 Made by: Fang-Jung Yang Received: 03/10/21 Academia Sinica, Taiwan ----------------------------------------------------------------------------- Strain: BRC566 Species: C. elegans Genotype: antIs31 II; unc-119(ed9) III. Description: antIs31 [attP-f + Cbr-unc-119(ant40) + glh-2p::phiC31 + rol-6(partial) + myo-2p::GFP + attP-r] II. Unc. antIS31 has been found to self-excise; check for GFP expression periodically to retain the insertion. GFP expression in pharynx is very weak (as it is in single copy) and is easiest to see during the L1-L3 stages. This strain contains a phiC31 docking site and can be used for precise single-copy integration of transgenes via recombination mediated cassette exchange. The docking site contains inverted phiC31-attP sites flanking phiC31 integrase expressed from the glh-2 germline promoter. Integration constructs need to have inverted phiC31-attB sites that flank the intended sequence to be inserted. antIs31 was derived by CRISPR/Cas9 knockout of Cbr-unc-119 in antIs30 creating ant40, a 691 bp deletion in Cbr-unc-119. Because antIs31 does not rescue unc-119(ed3), BRC566 facilitates the use of Unc-119 rescue as a selection marker for transgene insertions. Reference: Yang FJ, et al. "phiC31 integrase for recombination mediated single copy insertion and genome manipulation in C. elegans." Genetics 2021. Mutagen: Outcrossed: x4 Made by: Fang-Jung Yang Received: 04/14/21 Academia Sinica, Taiwan ----------------------------------------------------------------------------- Strain: BS1175 Species: Caenorhabditis elegans Genotype: cdk-4(gv3) X; ozEx76. Description: ozEx76 [cdk-4p::CDK-4::GFP + sur-5::DsRed)]. Maintain by picking DsRed+. ozEx76 is unstable; strain exhibits high levels of lethality and sterilty. ~10% of progeny are fertile. Reference: Fox PM, et al. Development. 2011 Jun;138(11):2223-34. Mutagen: Outcrossed: x Made by: PM Fox Received: 07/26/12 Washington University School of Medicine, St. Louis MO, USA ----------------------------------------------------------------------------- Strain: BS14 Species: Caenorhabditis elegans Genotype: gld-1(q266)/nDf24 I. Description: Heterozygotes are fertile and grow more slowly than gld-1(q266) homozygotes. gld-1(q266) homozygotes are sterile and produce small abnormal oocytes. nDf24 homozygotes are dead. Mutagen: Outcrossed: x Made by: Received: 01/21/09 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: BS3156 Species: Caenorhabditis elegans Genotype: unc-13(e51) gld-1(q485)/hT2 [dpy-18(h662)] I; +/hT2 [bli-4(e937)] III. Description: Heterozygotes are wild-type and segregate WT heterozygotes, Unc (unc-13 gld-1 homozygotes), and Dpy (hT2 homozygotes; the bli-4 mutation is suppressed by dpy-18). XX Uncs have tumorous germline and are sterile; XO Uncs are cross fertile (though they don't mate due to the Unc mutation). q485 is the canonical allele of gld-1. It behaves like deletions of the locus in complementation tests and thus is genetically null. Molecularly, it is a deletion of 82 bases and fails to produce gld-1 RNA and protein. Mutagen: Psoralen Outcrossed: x8 Made by: David Miller Received: 02/13/97 Washington University School of Medicine, St. Louis MO, USA ----------------------------------------------------------------------------- Strain: BS3164 Species: Caenorhabditis elegans Genotype: unc-32(e189) glp-1(ar202) III. Description: Temperature sensitive. Maintain at 15 degrees. Germline tumor formation at 25 degrees. Proximal proliferations defects. Reference: Pepper et al. (2003) Genetics 163(1):115-32. Mutagen: Outcrossed: x Made by: Jane Hubbard Received: 07/06/10 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: BS3347 Species: Caenorhabditis elegans Genotype: lin-45(dx84) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Unc heterozygotes, non-Unc Steriles (lin-45 homozygotes), larval lethals, and dead eggs. dx84 is a strong lin-45 raf allele: dx84 homozygotes from dx84/+ heterozygotes are 47% larval lethal and among the adults, 100% are Sterile and Vul. Mutagen: UV Outcrossed: x4 Made by: Eric Lambie Received: 06/14/02 Washington University School of Medicine, St. Louis, MO ----------------------------------------------------------------------------- Strain: BS3348 Species: Caenorhabditis elegans Genotype: C07A4.1(ok144) X. Description: Primers used to detect the deletion: IQ789.E1: CACACATCGGTCTTCCACAC. IQ789.E2: AATGAAACACCGGAAACTCG. IQ789.I1: TGCAATTGTGTTGCAGGAAT. IQ789.I2: AAAAGAGTGGCTGGCTCGTA. Mutagen: Outcrossed: x1 Made by: Momoyo Hanazawa Received: 06/09/03 Washington University School of Medicine, St. Louis MO, USA ----------------------------------------------------------------------------- Strain: BS3383 Species: Caenorhabditis elegans Genotype: pmk-3(ok169). Description: F42G8.4. No obvious phenotype. Follow by PCR. Predicted gene is a p38 related Map Kinase. Approx. 1.5 kb deletion by agarose gel (not sequenced so end points not known). Nested PCR primers for detecting F42G8.4: F42G8.4EL1 5' - TCGCCCTTTGTATGTCTTCC - 3'. F42G8.4ER1 5' - TTCTCCAGGGATTAACGGTG - 3'. F42G8.4IL1 5' - TTTTCACTGCGTCTCAATCG - 3'. F42G8.4IR1 5' - TTTCAAATTTGCAGGTGTGC - 3'. Mutagen: Outcrossed: x1 Made by: Barstead/Moulder Received: 01/27/00 Washington University School of Medicine, St. Louis MO, USA ----------------------------------------------------------------------------- Strain: BS3392 Species: Caenorhabditis elegans Genotype: gld-2(q497) gld-1(q485)/hT2 [dpy-18(h662)] I; unc-32(e189) glp-1(q175)/hT2 [bli-4(e937)] III. Description: Heterozygotes are wild-type and segregate WT heterozygotes, Unc (gld-2 gld-1; unc-32 glp-1 homozygotes), and Dpy (hT2 homozygotes; the bli-4 mutation is suppressed by dpy-18). Check Unc-32 animals for tumors to confirm presence of glp-1 in the line. glp-1(q175) is nonsense R191 > stop (opal). Mutagen: Outcrossed: x Made by: Dave Hansen Received: 10/15/12 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: BS3493 Species: Caenorhabditis elegans Genotype: gld-3(ok308)/mIn1 [dpy-10(e128) mIs14] II. Description: Homozygous sterile mutation balanced by GFP- and dpy-10-marked inversion. Heterozygotes are wild-type with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP ok308 homozygotes (mostly sterile or Mel, but can be slowly propagated at 20C). Pick WT dim GFP and check for correct segregation of progeny to maintain. Mutagen: UV/TMP Outcrossed: x3 Made by: Dave Hansen Received: 09/05/02 Washington University School of Medicine, St. Louis MO, USA ----------------------------------------------------------------------------- Strain: BS3727 Species: C. elegans Genotype: lip-1(ok154) IV. Description: Temperature-sensitive allele. Can be grown at 20C with reduced fertility. Defects in pachytene progression, small oocyte formation and Emo are highly penetrant at 25C. ok154 is a null allele; 1504 bp deletion from -156 bp to +1348 bp, removes the start codon. Reference: Proc Natl Acad Sci USA. Das D, et al. 2022 Jan 18;119(3):e2113649119. PMID: 35022236 Mutagen: Outcrossed: x8 Made by: Schedl Lab Received: 03/09/22 MD Anderson Cancer Center - Univ of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: BS3760 Species: C. elegans Genotype: rskn-1(ok159) I. Description: Ectopic ERK MPK-1 (detected by dpMPK-1 immunostaining) in the loop region. Reference: Proc Natl Acad Sci USA. Das D, et al. 2022 Jan 18;119(3):e2113649119. PMID: 35022236 Mutagen: Outcrossed: x5 Made by: Schedl Lab Received: 03/09/22 MD Anderson Cancer Center - Univ of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: BS509 Species: Caenorhabditis elegans Genotype: ozDf2/dpy-21(e428) par-4(it33) V. Description: Heterozygotes are wild-type and segregate wild-type (heterozygotes), Dpys (dpy-21 par-4 homozygotes that lay dead eggs), and dead eggs (ozDf2 homozygotes). Maintain by picking wild-type. Mutagen: Outcrossed: x Made by: Tim Schedl Received: 07/17/90 Washington University School of Medicine, St. Louis MO, USA ----------------------------------------------------------------------------- Strain: BS518 Species: Caenorhabditis elegans Genotype: ozDf1/sdc-3(y52y180) unc-76(e911) V. Description: Heterozygotes are slow growing with WT phenotype. Hets segregate more slow growing WT, embryonic lethals (ozDf1/ozDf1) and DpyUncs which are sick and have a maternal effect lethal (none of the offspring from the DpyUncs survive to reproduce). Maintain by picking WT. Mutagen: Outcrossed: x Made by: Tim Schedl Received: 07/17/90 Washington University School of Medicine, St. Louis MO, USA ----------------------------------------------------------------------------- Strain: BS5182 Species: Caenorhabditis elegans Genotype: sec-61.G(oz1) sma-1(e30) V/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Unc, dead eggs and Sma. sec-61.G sma-1 homozygotes grow up to be sterile adults, with endomitotic oocytes in the gonad arm. sec-61.G also known as emo-1. Mutagen: Tc1 Outcrossed: x10 Made by: Tim Schedl Received: 11/12/98 Washington University School of Medicine, St. Louis MO, USA ----------------------------------------------------------------------------- Strain: BS5183 Species: Caenorhabditis elegans Genotype: lin-45(oz201) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Unc, non-Unc Steriles, larval lethals, and dead eggs. oz201 is a strong lin-45 raf allele: oz201 homozygotes from oz201/+ heterozygotes segregate 55% larval lethal and the remaining adults are all Sterile and Vul. Mutagen: EMS Outcrossed: x4 Made by: Tim Schedl Received: 06/14/02 Washington University School of Medicine, St. Louis, MO ----------------------------------------------------------------------------- Strain: BS5351 Species: Caenorhabditis elegans Genotype: nos-2(ok230) nos-1(gv5)/mIn1 [dpy-10(e128) mIs14] II. Description: Homozygous sterile mutation balanced by GFP- and dpy-10-marked inversion. Heterozygotes are wild-type with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP nos-2 nos-1 homozygotes (segregate many dead embryos). Pick wild-type dim GFP and check for correct segregation of progeny to maintain. Reference: Hansen D, et al. Development. 2004 Jan;131(1):93-104. Mutagen: Outcrossed: x0 Made by: Dave Hansen Received: 05/02/14 Washington University School of Medicine, St. Louis MO, USA ----------------------------------------------------------------------------- Strain: BS5431 Species: Caenorhabditis elegans Genotype: prp-17(oz273) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Homozygous sterile mutation balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP sterile oz273 homozygotes. Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Mutagen: Outcrossed: x Made by: Jessica Kerins Received: 02/08/11 Washington University School of Medicine, St. Louis MO, USA ----------------------------------------------------------------------------- Strain: BS5435 Species: Caenorhabditis elegans Genotype: prp-17(oz273) I/hT2 (I;III); glp-1(oz264) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: glp-1(oz264) is a gain-of-function allele. Homozygous sterile mutation balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP sterile oz273; oz264 homozygotes. Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Mutagen: Outcrossed: x Made by: Jessica Kerins Received: 02/08/11 Washington University School of Medicine, St. Louis MO, USA ----------------------------------------------------------------------------- Strain: BS553 Species: Caenorhabditis elegans Genotype: fog-2(oz40) V. Description: Male/female strain. Maintain by mating. Mutagen: Psoralen Outcrossed: x8 Made by: Tim Schedl Received: 09/25/07 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: BS5633 Species: C. elegans Genotype: ieSi64 II; lag-1(oz536oz537[lag-1::degron::3xHA]) IV. Description: ieSi64 [gld-1p::TIR1::mRuby::gld-1 3'UTR + Cbr-unc-119(+)] II. Essentially wild-type when maintained on NGM plates. All germline stem cells enter into meiosis when treated with Auxin (1mM). References: Chen J, et al. PLoS Genet. 2020 Mar 20;16(3):e1008650. PMID: 32196486. Chen J, et al. 2020 MicroPublication (https://doi.org/10.17912/micropub.biology.000310) . Mutagen: Outcrossed: x2 Made by: Jian Chen Received: 09/10/21 Washington University School of Medicine, St. Louis MO, USA ----------------------------------------------------------------------------- Strain: BS585 Species: Caenorhabditis elegans Genotype: unc-13(e51) ozDf5 I; nDp4 (I;V)/+. Description: Strain gives WT hermaphrodites and dead eggs. Mutagen: gamma irradiation Outcrossed: x6 Made by: Tim Schedl Received: 09/20/93 Washington University School of Medicine, St. Louis MO, USA ----------------------------------------------------------------------------- Strain: BS7011 Species: Caenorhabditis elegans Genotype: nemp-1(oz534)/sC1(s2023) [dpy-1(s2170) umnIs41] III. Description: Segregates WT RFP+ heterozygotes, viable non-RFP nemp-1(oz534) homozygotes, and RFP+ Dpy. Maintain by picking wild-type RFP+. About 10-20% of nemp-1(oz534) homozygotes are sterile. Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Ariz Mohammad Received: 12/28/18 Washington University School of Medicine, St. Louis MO, USA ----------------------------------------------------------------------------- Strain: BS7012 Species: Caenorhabditis elegans Genotype: nemp-1(oz535)/sC1(s2023) [dpy-1(s2170) umnIs41] III. Description: Segregates WT RFP+ heterozygotes, viable non-RFP nemp-1(oz535) homozygotes, and RFP+ Dpy. Maintain by picking wild-type RFP+. About 10-20% of nemp-1(oz535) homozygotes are sterile. Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Ariz Mohammad Received: 12/28/18 Washington University School of Medicine, St. Louis MO, USA ----------------------------------------------------------------------------- Strain: BT12 Species: Caenorhabditis elegans Genotype: him-4(rh319) X. Description: Him. Reference: Vogel BE, Hedgecock EM. (2001) Development. 128:883-94. Mutagen: EMS Outcrossed: x2 Made by: Bruce Vogel Received: 03/31/15 University of Maryland Baltimore, Baltimore MD, USA ----------------------------------------------------------------------------- Strain: BT14 Species: Caenorhabditis elegans Genotype: fbl-1(hd43)/dpy-20(e1282) unc-24(e138) IV. Description: Heterozygotes are WT and segregate WT, Steriles (hd43 homozygotes) and Dpy Uncs. Mutagen: EMS Outcrossed: x6 Made by: Hutter/Muriel Received: 02/15/06 University of Maryland Baltimore, Baltimore MD, USA ----------------------------------------------------------------------------- Strain: BT24 Species: Caenorhabditis elegans Genotype: rhIs23 III. Description: rhIs23 [GFP::him-4] III. GFP-tagged full-length hemicentrin reporter. Reference: Vogel BE, Hedgecock EM. (2001) Development. 128:883-94. Mutagen: Outcrossed: x Made by: Bruce Vogel Received: 03/31/15 University of Maryland Baltimore, Baltimore MD, USA ----------------------------------------------------------------------------- Strain: BU7222 Species: Caenorhabditis elegans Genotype: pat-3(st564) III; kqEx73. Description: kqEx73 [pat-3(sp) + rab-3::RFP + cki-1::GFP]. Pick RFP+ to maintain. kqEx73 carries a form of pat-3 gene with splicing defects; rescues pat-3 null allele. pat-3(sp) is a frameshift mutation in the splice acceptor region (ag to aa) that abolishes conserved interaction domains such as the NPxY motifs and creates a splice variant with an extra 19 amino acids. The pat-3(sp) animals not only produce mutant pat-3, but also express the regular splice form due to utilization of an unusual splice acceptor. Abnormal Distal Tip Cell migration and pat-3 gene splicing (intron 7) defects. Reference: Kihira S, et al. PLoS One. 2012;7(8):e42425. Mutagen: none Outcrossed: x0 Made by: Shingo Kihira Received: 07/17/19 Baylor University, Waco, TX ----------------------------------------------------------------------------- Strain: BU8041 Species: C. elegans Genotype: pat-3(kq8041) III. Description: Mild motility and gonad migration defects. pat-3(kq8041) is an engineered Y804A substitution of the membrane distal tyrosine in the cytoplasmic domain. Reference: Hanna J, et al., microPublication Biology. 10.17912/micropub.biology.000291. https://www.micropublication.org/journals/biology/micropub-biology-000291 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Zhongqiang Qiu Received: 01/16/25 Baylor University, Waco, TX ----------------------------------------------------------------------------- Strain: BU8042 Species: Caenorhabditis elegans Genotype: pat-3(kq8042) III. Description: Motility and cell (DTC) migration defects. pat-3(kq8042) is an engineered Y804E substitution of the membrane distal tyrosine in the cytoplasmic domain. Reference: Hanna J, et al., microPublication Biology. 10.17912/micropub.biology.000291. https://www.micropublication.org/journals/biology/micropub-biology-000291 Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Zhongqiang Qiu Received: 01/16/25 Baylor University, Waco, TX ----------------------------------------------------------------------------- Strain: BU8043 Species: Caenorhabditis elegans Genotype: pat-3(kq8043) III. Description: Mild motility and cell migration defects. pat-3(kq8043) is an engineered Y804F substitution of the membrane distal tyrosine in the cytoplasmic domain. Reference: Hanna J, et al., microPublication Biology. 10.17912/micropub.biology.000291. https://www.micropublication.org/journals/biology/micropub-biology-000291 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Zhongqiang Qiu Received: 01/16/25 Baylor University, Waco, TX ----------------------------------------------------------------------------- Strain: BV70 Species: C. elegans Genotype: unc-119(ed3) III; zbIs2. Description: zbIs2 [pie-1p::lifeact::RFP::pie-1 3' UTR + unc-119(+)]. Lifeact tag labels F-actin without interfering F-actin dynmics. The strain has never been officially published; however, a GFP version of this transgene is described in Pohl C & Bao Z. Dev Cell. 2010 Sep 14;19(3):402-12. PMID: 20833362. Mutagen: Bombardment Outcrossed: x0 Made by: Zhirong Bao Received: 12/07/22 Memorial Sloan-Kettering Cancer Center, New York, NY ----------------------------------------------------------------------------- Strain: BW1039 Species: Caenorhabditis elegans Genotype: dpy-5(e61) unc-40(e271) I. Description: DpyUnc. Slightly Egl. Mutagen: Outcrossed: x Made by: Paul Mains Received: 02/24/89 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1046 Species: Caenorhabditis elegans Genotype: unc-37(e262) daf-8(e1393) I. Description: Unc. Constitutive dauer formation (Daf-c) at 25C. Mutagen: Outcrossed: x Made by: Paul Mains Received: 02/24/89 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1049 Species: Caenorhabditis elegans Genotype: unc-40(e271) bli-4(e937) I. Description: UncBli Strain. Blisters late. Mutagen: Outcrossed: x Made by: Paul Mains Received: 02/24/89 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1053 Species: Caenorhabditis elegans Genotype: dpy-14(e188) unc-13(e1091) I. Description: Unc. Temperature-sensitive Dpy. Grows best at 15C. Mutagen: Outcrossed: x Made by: Paul Mains Received: 02/24/89 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1084 Species: Caenorhabditis elegans Genotype: ncl-1(e1865) unc-36(e251) tra-1(e1099) dpy-18(e364) III; ctDp6 (III;f). Description: Animals which carry ctDp6 are WT. Animals which have lost ctDp6 are DpyUnc Males. Maintain by picking WT. Mutagen: Gamma Rays Outcrossed: x Made by: Craig Hunter Received: 04/06/93 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1102 Species: Caenorhabditis elegans Genotype: dpy-5(e61) mei-2(ct102) unc-29(e1072) I; sDp2 (I;f). Description: Animals with the duplication are Unc. Animals that have lost the duplication are DpyUncMel. mei-2 is non-conditional recessive maternal effect lethal. Mutagen: EMS Outcrossed: x8 Made by: Mains & Sulston Received: 01/21/91 University of Calgary, Calgary, Alberta ----------------------------------------------------------------------------- Strain: BW1118 Species: Caenorhabditis elegans Genotype: mel-25(ct60) unc-42(e270)/unc-23(e25) vab-8(e1017) V; lon-2(e678) X. Description: Maintain by picking Lon non-Unc. Throws Lon non-Unc, Lon Unc Vab (bent head, tails very thin), and Lon Unc Mel (kinker, temperature-sterile). Mutagen: EMS Outcrossed: x13 Made by: Mains P Received: 01/21/91 University of Calgary, Calgary, Alberta ----------------------------------------------------------------------------- Strain: BW1145 Species: Caenorhabditis elegans Genotype: tra-1(e1099) dpy-18(e364) III; ctDp2 (III;f). Description: Animals which carry ctDp2 are WT. Animals which have lost ctDp2 are Dpy Males. Maintain by picking WT. Mutagen: Gamma Rays Outcrossed: x Made by: Craig Hunter Received: 04/06/93 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1161 Species: Caenorhabditis elegans Genotype: unc-22(ct169) IV. Description: Twitcher. Mutagen: Trimethylpsoralen Outcrossed: x0 Made by: Mark Yandell Received: 10/18/95 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1163 Species: Caenorhabditis elegans Genotype: unc-22(ct171) IV. Description: Twitcher. Mutagen: Trimethylpsoralen Outcrossed: x0 Made by: Mark Yandell Received: 01/18/96 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1166 Species: Caenorhabditis elegans Genotype: unc-22(ct174) IV. Description: Twitcher. Mutagen: Trimethylpsoralen Outcrossed: x0 Made by: Mark Yandell Received: 01/18/96 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1168 Species: Caenorhabditis elegans Genotype: unc-22(ct176) IV. Description: Twitcher. Mutagen: Trimethylpsoralen Outcrossed: x0 Made by: Mark Yandell Received: 10/18/95 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1169 Species: Caenorhabditis elegans Genotype: unc-22(ct177) IV. Description: Twitcher. Mutagen: Trimethylpsoralen Outcrossed: x0 Made by: Mark Yandell Received: 01/18/96 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1176 Species: Caenorhabditis elegans Genotype: unc-22(ct181) IV. Description: Mutagen: Trimethylpsoralen Outcrossed: x0 Made by: Mark Yandell Received: 01/18/96 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1199 Species: Caenorhabditis elegans Genotype: him-8(e1489) IV; unc-42(e270) sma-1(e30) V; ctDp8[vab-8(e1017)] (V;f). Description: Mutagen: Outcrossed: x Made by: Hunter C Received: 12/04/90 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1341 Species: Caenorhabditis elegans Genotype: nob-1(ct223) III; eDp6 (III;f). Description: Animals with the duplication are WT. Animals without the duplication are Nob (NO Back end; 100% lethal). Pick wild-type to maintain; typically segregates about 50% wild type progeny and 50% Nob larvae. Mutagen: Trimethylpsoralen Outcrossed: x Made by: Lois Edgar Received: 03/01/01 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1369 Species: Caenorhabditis elegans Genotype: unc-32(e189) dpy-18(e364) ctDf2/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Maintain by picking WT. Mutagen: Trimethylpsoralen Outcrossed: x0 Made by: Nurit Wolf Received: 01/18/96 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1434 Species: Caenorhabditis elegans Genotype: dpy-17(e164) III; him-8(e1489) IV; her-1(y101hv1) unc-42(e270) V; ctDp11 (III;V;f). Description: Wild type hermaphrodites and males which produce WT, Dpy, Unc and DpyUnc progeny due to mating or loss of the duplication. Pick WT hermaphrodites and males to maintain. Mutagen: Gamma Rays Outcrossed: x5 Made by: Craig Hunter Received: 06/17/99 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1535 Species: Caenorhabditis elegans Genotype: dpy-18(e364) ctDf3 unc-25(e156)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Maintain by picking WT. Mutagen: Trimethylpsoralen Outcrossed: x0 Made by: Nurit Wolf Received: 01/18/96 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1561 Species: Caenorhabditis elegans Genotype: dpy-18(e364) nob-1(ct223) unc-25(e156) III; eDp6 (III;f). Description: Animals with the duplication are WT. Animals without the duplication are Nob (NO Back end; 100% lethal). Pick wild-type to maintain. The Dpy and Unc phenotypes are not visible in the Nob background. ct223 is recessive. Mutagen: Trimethylpsoralen Outcrossed: x Made by: Lois Edgar Received: 01/16/01 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1563 Species: Caenorhabditis elegans Genotype: pal-1(ct281)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Pick wild-type heterozygotes to maintain. ct281 homozygotes show Nob phenotype: approximately 80% of homozygous embryos arrest at about the time of hatching with fairly normal anterior development but a severely deformed posterior with a variable knob-like shape; approximately 20% fail to enclose and do not hatch. ct281 is a 4.7kb deletion removing intron 5, exon 6, and the 3'UTR of the pal-1 gene. Reference: Edgar LG, et al. Dev Biol. 2001 Jan 1;229(1):71-88. Mutagen: Outcrossed: x Made by: Lois Edgar Received: 03/09/17 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1566 Species: Caenorhabditis elegans Genotype: pal-1(ct224)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Pick wild-type heterozygotes to maintain. ct224 homozygotes show Nob phenotype: approximately 80% of homozygous embryos arrest at about the time of hatching with fairly normal anterior development but a severely deformed posterior with a variable knob-like shape; approximately 20% fail to enclose and do not hatch. ct224 is a 4.2kb deletion removing exon 1 through exon 6 of the pal-1 gene. Reference: Edgar LG, et al. Dev Biol. 2001 Jan 1;229(1):71-88. Mutagen: Outcrossed: x Made by: Lois Edgar Received: 03/09/17 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW163 Species: Caenorhabditis elegans Genotype: ctDf1 V/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Uncs (heterozygotes) and dead eggs (ctDf1 homozygotes or nT1 homozygotes). Mutagen: Gamma Rays Outcrossed: x Made by: Received: 05/01/84 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1634 Species: Caenorhabditis elegans Genotype: nob-1(ct223)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: WT hermaphrodites that segregate WT, Dpy Steriles and Nob larvae. The Nob phenotype results in 100% lethality. Maintain by picking WT. Mutagen: Outcrossed: x Made by: Received: 03/01/01 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1651 Species: Caenorhabditis elegans Genotype: nob-1(ct351) III; eDp6 (III;f). Description: Animals with the duplication are WT. Animals without the duplication are Nob (NO Back end; 100% lethal). Pick wild-type to maintain; segregates wild type progeny, Nob larvae, and occasional dead eggs. ct351 is recessive. Mutagen: Trimethlypsoralen Outcrossed: x Made by: Lois Edgar Received: 01/16/01 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1739 Species: Caenorhabditis elegans Genotype: +/eT1 III; unc-62(t2012) dpy-11(e224)/eT1 V. Description: Heterozygotes are wild-type, and segregate WT heterozygotes, Dpy Mel (unc-62 dpy-11 homozygotes), and dead eggs (eT1 homozygotes). unc-62 dpy-11 homozygotes give only dead embryos (96%) or dead larva (4%). Mutagen: Outcrossed: x8 Made by: R. Feitchinger Received: 01/07/04 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1747 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177) let-427(s1057)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36, Sterile DpyUncs and dead eggs. Maintain by picking WT. Mutagen: from BC3006 Outcrossed: x Made by: Bill Wood Received: 10/21/96 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1809 Species: Caenorhabditis elegans Genotype: gpa-16(it143) I; him-5(e1490) V. Description: Temperature-sensitve. Maintain at 15C. Slight Maternal Effect Lethal (Mel) at 15C, more pronounced at 20C. Highly penetrant Mel at 25C and a fraction of the survivors have reversed left-right organs. Mutagen: Outcrossed: x10 Made by: D. Bergmann Received: 01/16/04 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1850 Species: Caenorhabditis briggsae Genotype: Cbr-him(s1290). Description: C. briggsae strain. Hermaphrodites are variably self-sterile, which is overcome by mating to males of the same genotype. From BC1991. Mutagen: Outcrossed: x2 Made by: Adrian Streit Received: 06/17/99 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1927 Species: C. elegans Genotype: pal-1(ct224)/qC1 [dpy-19(e1259) glp-1(q339)] III; ctIs33. Description: ctIs33 [pal-1::GFP + rol-6(su1006)]. Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Pick wild-type heterozygotes to maintain. ct224 homozygotes show Nob phenotype: approximately 80% of homozygous embryos arrest at about the time of hatching with fairly normal anterior development but a severely deformed posterior with a variable knob-like shape; approximately 20% fail to enclose and do not hatch. ct224 is a 4.2kb deletion removing exon 1 through exon 6 of the pal-1 gene. ctIs33 carries a non-rescuing pal-1::GFP fusion containing ~7kb 5' of the SL1 splice site through part of exon 5 fused to GFP. GFP expression is primarily embryonic and limited to a few cells; not visible except at high magnification. Reference: Edgar LG, et al. Dev Biol. 2001 Jan 1;229(1):71-88. Mutagen: TMP/UV Outcrossed: x4 Made by: Lois Edgar Received: 03/22/17 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1932 Species: Caenorhabditis elegans Genotype: ctIs39. Description: ctIs39 [hbl-1p::GFP::NLS::hbl-1 3'UTR + rol-6(su1006)]. Rollers. Reporter encodes the first 133 amino acids of HBL-1 followed by GFP with SV40 NLS and 1.4 kb of hbl-1 3' UTR. Reference: Fay DS, et al. Dev Biol. 1999 Jan 15;205(2):240-53. Mutagen: Gamma Rays Outcrossed: x3 Made by: David Fay Received: 04/14/06 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1935 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; ctIs43 him-5(e1490) V. Description: ctIs43 [dbl-1p::GFP + dbl-1p::GFP::NLS + unc-119(+)]. Mutagen: Gamma Outcrossed: x2 Made by: Yo Suzuki Received: 08/04/03 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1940 Species: Caenorhabditis elegans Genotype: ctIs40 X. Description: ctIs40 [dbl-1(+) + sur-5::GFP]. Lon. dbl-1 over-expressing line from injection of constructs ZC421 and pTG98. Mutagen: Outcrossed: x3 Made by: Yo Suzuki Received: 01/21/03 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1943 Species: Caenorhabditis elegans Genotype: gad-1(ct226) dpy-11(e224) V. Description: Maintain at 15C. Temperature-sensitive maternal effect, gastrulation defective. 100% of embryos arrest without gastrulating at 25C. Mutagen: EMS Outcrossed: x6 Made by: Bill Wood Received: 10/09/98 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW1946 Species: Caenorhabditis elegans Genotype: ctIs43 unc-42(e270) V. Description: Unc. ctIs43 [dbl-1p::GFP + dbl-1p::GFP::NLS + unc-119(+)]. Mutagen: Outcrossed: x3 Made by: Yo Suzuki Received: 08/04/03 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW2027 Species: Caenorhabditis elegans Genotype: unc-62(ct344) V; yDp1 (IV;V;f). Description: Animals carrying the duplication are wild-type. Animals which have lost yDp1 are Unc and give only dead eggs(57%) or dead larvae(43%). Mutagen: Outcrossed: x Made by: Queta Boese Received: 12/22/03 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW2041 Species: Caenorhabditis elegans Genotype: pal-1(ct224) dpy-17(e164) ncl-1(e1865) unc-36(e251) III; svDp1 (III;f). Description: svDp1 [sur-5::GFP] (III;f). svDp1 was derived by insertion of a sur-5::GFP marker into sDp3(III;f). Pick GFP+ wild-type to maintain. svDp1 rescues ct224. ct224 homozygotes (lacking GFP expression) show Nob phenotype: approximately 80% of homozygous embryos arrest at about the time of hatching with fairly normal anterior development but a severely deformed posterior with a variable knob-like shape; approximately 20% fail to enclose and do not hatch. The duplication is lost in about 30-40% of embryos. Reference: Edgar LG, et al. Dev Biol. 2001 Jan 1;229(1):71-88. Mutagen: Outcrossed: x>5 Made by: Lois Edgar Received: 03/09/17 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW2063 Species: Caenorhabditis elegans Genotype: ceh-20(ay38) unc-36(e251) III; svDp1 (III;f). Description: May have unc-4(e120) mutation in background. svDp1 balances from pal-1 through unc-36 on III. svDp1 made by fusing array containing [unc-4(+) + sur-5::GFP] to sDp3(III;f). Mutagen: Outcrossed: x Made by: Tassa Saldi Received: 01/07/04 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW219 Species: Caenorhabditis elegans Genotype: nDf31/unc-23(e25) sma-1(e30) V. Description: Heterozygotes are WT and segregate SmaUnc and Lethals. Lethal in L1. Maintain by picking WT. Mutagen: Gamma Rays Outcrossed: x Made by: Trent C Received: 06/01/84 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW287 Species: Panagrolaimus sp. Genotype: Caenorhabditis briggsae. Description: Chinese rhabditis hermaphrodite nematode from Bill Wood's Chinese collection, Beijing. According to David Fitch this strain is Panagrolaimus sp. April 2008: According to sequence data from Howe & Denver 2008 BMC Evol Biol 8:62, strain BW287 is C. briggsae. Mutagen: Outcrossed: x Made by: Bill Wood Received: 01/21/03 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW315 Species: Caenorhabditis elegans Genotype: mig-10(ct41) III. Description: Withered tail and Egl. Adults shorter than WT. Embryonic cell migrations affected: ALM and HSN with high penetrance, CAN with moderate penetrance. All misplaced nuclei at positions indicating incomplete migration. Mutagen: EMS Outcrossed: x Made by: Jim Manser Received: 01/21/93 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW462 Species: Caenorhabditis elegans Genotype: sup-26(ct49) III. Description: Superficially wild-type. Maintain under normal conditions. Reference: Manser J, et al. (2002) Genesis 34(3):184-95. Mutagen: EMS Outcrossed: x Made by: Bill Wood Received: 09/17/10 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: BW506 Species: Caenorhabditis elegans Genotype: ceh-10(ct78) III. Description: Withered tail. Adults shorter than WT. Embryonic cell migrations affected: CAN migration with high penetrance. Previously called mig-11. Mutagen: EMS Outcrossed: x1 Made by: Eve Wolinsky Received: 01/21/93 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW538 Species: Caenorhabditis elegans Genotype: unc-13(e1091) lin-10(e1438) I. Description: Unc and Vul. Mutagen: Outcrossed: x Made by: Paul Mains Received: 02/24/89 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW54 Species: Caenorhabditis elegans Genotype: ct350 II. Description: Maintain at 15C. Temperature sensitive embryonic lethal. Sterile at 25C. Congenic strain, N2 and Bergerac BE. This allele should NOT be assumed to define a gene to which someone gave the name zyg-12. This name should not be used unless someone finds a non-complementing Bristol mutation. There is no evidence at present that the ts results from a single gene defect. Has been backcrossed >6 times to Bristol strains and should only contain Bergerac DNA in the unc-85 to dpy-10 region. Mutagen: Outcrossed: x>6 Made by: B. Vanderslice Received: 01/21/93 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW589 Species: Caenorhabditis elegans Genotype: lin-10(e1438) unc-29(e1072) I. Description: Vul and Unc. Mutagen: Outcrossed: x Made by: Paul Mains Received: 02/24/89 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW635 Species: Caenorhabditis elegans Genotype: lin-10(e1438) daf-8(e1393) I. Description: Maintain at 15C. Vulvaless. Temperature-sensitive constitutive dauer (Daf-c) at 25C. Mutagen: Outcrossed: x Made by: Paul Mains Received: 02/24/89 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW708 Species: Caenorhabditis elegans Genotype: lin-28(n719) daf-8(e1393) I. Description: Egl. Constitutive dauer (Daf-c) at 25C. Mutagen: Outcrossed: x Made by: Paul Mains Received: 02/24/89 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW774 Species: Caenorhabditis elegans Genotype: lin-28(n719) unc-29(e1072) I. Description: Egl and Unc. Mutagen: Outcrossed: x Made by: Paul Mains Received: 02/24/89 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW837 Species: Caenorhabditis elegans Genotype: unc-29(e1072) lin-11(n566) I. Description: Vul and Unc. Mutagen: Outcrossed: x Made by: Paul Mains Received: 02/24/89 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BW916 Species: Caenorhabditis elegans Genotype: sup-26(n1091) III. Description: Superficially wild-type. Maintain under normal conditions. Reference: Manser J, et al. (2002) Genesis 34(3):184-95. Mutagen: EMS Outcrossed: x Made by: Bill Wood Received: 09/17/10 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: BW983 Species: Caenorhabditis elegans Genotype: bli-4(e937) unc-37(e262) I. Description: BliUnc. Blister seen in old adults. Mutagen: Outcrossed: x Made by: Paul Mains Received: 02/24/89 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: BX10 Species: Caenorhabditis elegans Genotype: ads-1(wa3) III. Description: Deficient in synthesis of ether-linked lipids. Contains high content of saturated fatty acids. Reference: Shi X, et al. J Lipid Res. 2016 Feb;57(2):265-75. Mutagen: EMS Outcrossed: x4 Made by: Jennifer Watts Received: 06/15/16 Washington State University, Pullman WA ----------------------------------------------------------------------------- Strain: BX106 Species: Caenorhabditis elegans Genotype: fat-6(tm331) IV. Description: Received new stock with confirmed deletion from Jennifer Watts 07/16/2009. Mutagen: UV/TP Outcrossed: x4 Made by: S. Mitani Received: 05/21/07 Washington State University, Pullman WA ----------------------------------------------------------------------------- Strain: BX107 Species: Caenorhabditis elegans Genotype: fat-5(tm420) V. Description: Received new stock with confirmed deletion from Jennifer Watts 07/16/2009. Mutagen: UV/TMP Outcrossed: x4 Made by: S. Mitani Received: 05/21/07 Washington State University, Pullman WA ----------------------------------------------------------------------------- Strain: BX110 Species: Caenorhabditis elegans Genotype: fat-6(tm331) IV; fat-5(tm420) V. Description: Mutagen: Outcrossed: x4 Made by: Trisha Brock Received: 01/06/10 Washington State University, Pullman WA ----------------------------------------------------------------------------- Strain: BX113 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; waEx15. Description: waEx15 [fat-7::GFP + lin15(+)]. Maintain by picking non-Muv animals. These should be GFP+. Mutagen: Outcrossed: x Made by: Trisha Brock Received: 04/29/09 Washington State University, Pullman WA ----------------------------------------------------------------------------- Strain: BX115 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; waEx16. Description: waEx16 [fat-6::GFP + lin15(+)]. Maintain by picking non-Muv animals. These should be GFP+. Mutagen: Outcrossed: x Made by: Trisha Brock Received: 04/29/09 Washington State University, Pullman WA ----------------------------------------------------------------------------- Strain: BX14 Species: Caenorhabditis elegans Genotype: elo-1(wa7) IV. Description: Reduced fatty acid elongation. Mutagen: EMS Outcrossed: x4 Made by: Jennifer Watts Received: 05/24/02 Washington State University, Pullman WA ----------------------------------------------------------------------------- Strain: BX150 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; waEx18. Description: waEx18 [fat-5::GFP + lin15(+)]. Maintain by picking non-Muv animals. These should be GFP+. Mutagen: Outcrossed: x Made by: Trisha Brock Received: 04/29/09 Washington State University, Pullman WA ----------------------------------------------------------------------------- Strain: BX153 Species: Caenorhabditis elegans Genotype: fat-7(wa36) V. Description: Mutagen: EMS Outcrossed: x6 Made by: Received: 12/11/06 Washington State University, Pullman WA ----------------------------------------------------------------------------- Strain: BX156 Species: Caenorhabditis elegans Genotype: fat-6(tm331) IV; fat-7(wa36) V. Description: Mutagen: Outcrossed: x Made by: Jennifer Watts Received: 01/06/10 Washington State University, Pullman WA ----------------------------------------------------------------------------- Strain: BX160 Species: Caenorhabditis elegans Genotype: fat-7(wa36) fat-5(tm420) V. Description: Mutagen: Outcrossed: x4 Made by: Trisha Brock Received: 01/06/10 Washington State University, Pullman WA ----------------------------------------------------------------------------- Strain: BX165 Species: Caenorhabditis elegans Genotype: nhr-80(tm1011) III. Description: Mutagen: UV/TMP Outcrossed: x3 Made by: S. Mitani Received: 12/02/08 Washington State University, Pullman WA ----------------------------------------------------------------------------- Strain: BX17 Species: Caenorhabditis elegans Genotype: fat-4(wa14) IV. Description: No delta5 fatty acid desaturase activity. Mutagen: EMS Outcrossed: x4 Made by: Jennifer Watts Received: 05/24/02 Washington State University, Pullman WA ----------------------------------------------------------------------------- Strain: BX24 Species: Caenorhabditis elegans Genotype: fat-1(wa9) IV. Description: No N3 fatty acid desaturase activity. Mutagen: EMS Outcrossed: x4 Made by: Jennifer Watts Received: 05/24/02 Washington State University, Pullman WA ----------------------------------------------------------------------------- Strain: BX259 Species: Caenorhabditis elegans Genotype: acl-7(wa20) II. Description: Deficient in synthesis of ether-linked lipids. Contains high content of saturated fatty acids. Reference: Shi X, et al. J Lipid Res. 2016 Feb;57(2):265-75. Mutagen: EMS Outcrossed: x4 Made by: Jennifer Watts Received: 06/15/16 Washington State University, Pullman WA ----------------------------------------------------------------------------- Strain: BX26 Species: Caenorhabditis elegans Genotype: fat-2(wa17) IV. Description: No delta 12 fatty acid desaturase activity. Slow growing. Unc. Dpy. Cold sensitive - maintain at 20C or higher. Mutagen: EMS Outcrossed: x4 Made by: Jennifer Watts Received: 05/24/02 Washington State University, Pullman WA ----------------------------------------------------------------------------- Strain: BX275 Species: Caenorhabditis elegans Genotype: fard-1(wa28) X. Description: Deficient in synthesis of ether-linked lipids. Contains high content of saturated fatty acids. Reference: Shi X, et al. J Lipid Res. 2016 Feb;57(2):265-75. Mutagen: EMS Outcrossed: x4 Made by: Jennifer Watts Received: 06/15/16 Washington State University, Pullman WA ----------------------------------------------------------------------------- Strain: BX30 Species: Caenorhabditis elegans Genotype: fat-3(wa22) IV. Description: Slow growing. Unc. Dpy. No delta6 fatty acid desaturase activity. Mutagen: EMS Outcrossed: x4 Made by: Jennifer Watts Received: 05/24/02 Washington State University, Pullman WA ----------------------------------------------------------------------------- Strain: BX52 Species: Caenorhabditis elegans Genotype: fat-4(wa14) fat-1(wa9) IV. Description: Mutagen: Outcrossed: x Made by: Received: 12/11/06 Washington State University, Pullman WA ----------------------------------------------------------------------------- Strain: BXN653 Species: Caenorhabditis elegans Genotype: scrm-1(cjn26) zdIs5 I. Description: zdIs5 [mec-4p::GFP + lin-15(+)] I. mec-4::GFP is expressed in touch neurons. Null allele of scrm-1: 2501 bp deletion and 7 bp insertion (CACACAT) removes the entire scrm-1 coding sequence (position 11688933 - 11691433 of chromosome I). Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Tarika Vijayaraghavan Received: 09/12/19 Monash University, Clayton, Victoria, Australia ----------------------------------------------------------------------------- Strain: BXN723 Species: Caenorhabditis elegans Genotype: fzo-1(cjn20) II. Description: Animals display reduced body bends and thrash rates, slow growth, and fragmented mitochondria. cjn20 is a 2629 bp deletion removing nucleotides 25-2654 of the fzo-1 locus. Originally published as cjn020. Reference: Byrne JJ, et al. Cell Mol Life Sci. 2019 May;76(10):1967-1985. (PMID 30840087) Mutagen: Crispr/Cas9 Outcrossed: x4 Made by: Joseph J. Byrne Received: 09/12/19 Monash University, Clayton, Victoria, Australia ----------------------------------------------------------------------------- Strain: BY250 Species: Caenorhabditis elegans Genotype: vtIs7. Description: vtIs7 [dat-1p::GFP]. Integrated reporter with bright GFP observable in all dopaminergic neurons and processes. No behavioral changes have been noted in relation to transgene integration. DA neuron degeneration is evident with easily detectible loss of GFP expression (e.g. Hardaway et al, J. Neurosci, 2015). Reference: Nass, R., et al. (2002). "Neurotoxin-induced degeneration of dopamine neurons in Caenorhabditis elegans." Proc Natl Acad Sci USA 99(5): 3264-3269. Mutagen: Outcrossed: x4 Made by: Richard Nass Received: 04/18/23 Florida Atlantic University, Jupiter, FL ----------------------------------------------------------------------------- Strain: BZ1202 Species: Caenorhabditis elegans Genotype: seb-3(eg696) X. Description: This strain is tolerant to acute treatment of ethanol. The severity and incidence of stress-induced tremors are greater than in wild-type. Reference: Jee C, et al. Genes Brain Behav. 2013 Mar;12(2):250-62. Mutagen: EMS Outcrossed: x4 Made by: Changhoon Jee Received: 08/29/16 Texas A&M University, College Station, TX ----------------------------------------------------------------------------- Strain: BZ142 Species: Caenorhabditis elegans Genotype: slo-1(eg142) V. Description: Head-bending phenotype. Maintain under normal conditions. Reference: Kim, H et al. (2009) PLoS Genetics 5(12):31000780. Mutagen: EMS Outcrossed: x2 Made by: Hoky Kim Received: 04/09/10 Rosalind Franklin University, North Chicago, IL ----------------------------------------------------------------------------- Strain: BZ28 Species: Caenorhabditis elegans Genotype: snf-6(eg28) III. Description: Head-bending phenotype. Maintain under normal conditions. Reference: Kim, H et al. (2004) Nature 430:891-6. Mutagen: EMS Outcrossed: x4 Made by: Hoky Kim Received: 04/09/10 Rosalind Franklin University, North Chicago, IL ----------------------------------------------------------------------------- Strain: BZ33 Species: Caenorhabditis elegans Genotype: dys-1(eg33) I. Description: Head-bending phenotype. Maintain under normal conditions. Reference: Kim, H et al. (2004) Nature 430:891-6. Mutagen: EMS Outcrossed: x4 Made by: Hoky Kim Received: 04/09/10 Rosalind Franklin University, North Chicago, IL ----------------------------------------------------------------------------- Strain: BZ555 Species: Caenorhabditis elegans Genotype: egIs1. Description: egIs1 [dat-1p::GFP] Bright GFP observable in dopamine neuronal soma and processes. For some reason this strain is resistant to neurotoxic effects of 6-OHDA compared to an independent strain BY200 (Nass et al., 2002). Or maybe BY200 is more sensitive to 6-OHDA due to its co-injection marker gene rol-6? Mutagen: TMP/UV Outcrossed: x6 Made by: Jon Pierce-Shimomura Received: 11/10/03 Gallo Center, UCSF, San Francisco, CA ----------------------------------------------------------------------------- Strain: BZ873 Species: Caenorhabditis elegans Genotype: aaim-1(ok295) X. Description: Dopamine receptor knockout. [NOTE: (3/3/2025) ok295 was previously described as an allele of dop-3/T14E8.4, but is actually an allele of aaim-1/T14E8.4.1 according to current gene models.] Derived by out-crossing parental strain RB563. Outer Left Sequence: ttgctccagcggttctagtt. Outer Right Sequence: gactgtctaagcgaccagcc. Inner Left Sequence: ttgtttgcgggtttgataca. Inner Right Sequence: agaagcacgcggtagttgat. Inner Primer PCR Length: 3254. Estimated Deletion Size: about 1000 bp. Mutagen: UV/TMP Outcrossed: x6 Made by: J. Pierce-Shimomura Received: 02/28/05 Gallo Center, UCSF, San Francisco, CA ----------------------------------------------------------------------------- Strain: BZ978 Species: Caenorhabditis elegans Genotype: islo-1(eg978) IV. Description: Head-bending phenotype. Maintain under normal conditions. Reference: Kim, H et al. (2009) PLoS Genetics 5(12):31000780. Mutagen: EMS Outcrossed: x2 Made by: Hoky Kim Received: 04/09/10 Rosalind Franklin University, North Chicago, IL ----------------------------------------------------------------------------- Strain: CA1117 Species: Caenorhabditis elegans Genotype: dsb-1(we11) IV/nT1[unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Uncs, dead eggs, and non-Uncs (dsb-1 homozygotes), which produce 99% inviable embryos due to meiotic nondisjunction. Pick Unc to maintain and check for correct segregation of progeny. we11 is a TCA to TAA nonsense mutation in the dsb-1 coding sequence that introduces a premature stop after leucine 96. Reference: Stamper EL, et al. PLoS Genet. 2013;9(8):e1003679. Mutagen: Outcrossed: x Made by: Ericca Stamper Received: 05/13/16 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1199 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; ieSi38 IV. Description: ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. Single copy transgene inserted into chromosome IV (cxTi10882) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in germ line and early embryos. This strain can be used for auxin-inducible degradation (AID) in germ line and early embryos. Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Mutagen: Outcrossed: x0 Made by: Ze Cheng Received: 09/19/16 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1200 Species: Caenorhabditis elegans Genotype: ieSi57 II; unc-119(ed3) III. Description: ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Single copy transgene inserted into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in the soma. This strain can be used for auxin-inducible degradation (AID) in somatic tissues. Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Mutagen: Outcrossed: x0 Made by: Liangyu Zhang Received: 12/02/15 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1202 Species: Caenorhabditis elegans Genotype: ieSi57 II; ieSi58 IV. Description: ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. ieSi58 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] IV. ieSi57 is a single copy transgene inserted into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in the soma. ieSi58 is a single copy transgene inserted into chromosome IV (oxTi177) expressing degron::GFP in the soma. This strain can be used as control for auxin-inducible degradation (AID) in somatic tissues. Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Mutagen: Outcrossed: x0 Made by: Liangyu Zhang Received: 12/02/15 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1203 Species: Caenorhabditis elegans Genotype: ieEx21. Description: ieEx21 [smu-2p::degron::smu-2::GFP::smu-2 3'UTR + rol-6(su1006)]. Rollers. Pick Rollers to maintain array. Rollers carry a transgene expressing degron- and GFP- tagged SMU-2 in both the soma and the germ line. This strain can be combined with different TIR1 strains to test auxin-inducible degradation (AID) of nuclear protein in various tissues. Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Mutagen: Outcrossed: x0 Made by: Liangyu Zhang Received: 12/02/15 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1204 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; ieSi58 IV. Description: ieSi58 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] IV. Single copy transgene inserted into chromosome IV (oxTi177) expressing degron::GFP in the soma. This strain can be combined with different TIR1 strains to test auxin-inducible degradation (AID) of protein in somatic tissue. Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Mutagen: Outcrossed: x0 Made by: Liangyu Zhang Received: 12/02/15 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1205 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; ieSi59 III. Description: ieSi59 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] III. Single copy transgene inserted into chromosome III (oxTi444) expressing degron::GFP at low levels in the soma. This strain can be combined with different TIR1 strains to test auxin-inducible degradation (AID) of protein in somatic tissue. Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Mutagen: Outcrossed: x0 Made by: Liangyu Zhang Received: 12/02/15 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1206 Species: Caenorhabditis elegans Genotype: ieSi57 II; ieSi59 III. Description: ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. ieSi59 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] III. ieSi57 is a single copy transgene inserted into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in the soma. ieSi59 is a single copy transgene inserted into chromosome III (oxTi444) expressing degron::GFP at low levels in the soma. This strain can be used as control for auxin-inducible degradation (AID) in somatic tissues. Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Mutagen: Outcrossed: x0 Made by: Liangyu Zhang Received: 12/02/15 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1207 Species: Caenorhabditis elegans Genotype: dhc-1(ie28[dhc-1::degron::GFP]) I. Description: A degron::GFP tag was inserted at the 3' end of the endogenous dhc-1 coding sequence via CRISPR/Cas9. This strain can be combined with different TIR1 strains to examine spatial and temporal requirements for dynein, and to serve as a control strain for auxin-inducible degradation (AID). Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Liangyu Zhang Received: 09/19/16 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1208 Species: Caenorhabditis elegans Genotype: ieSi60 II; unc-119(ed3) III. Description: ieSi60 [myo-2p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Single copy transgene inserted into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in pharyngeal muscle. This strain can be used for auxin-inducible degradation (AID) in pharyngeal muscle. Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Mutagen: Outcrossed: x0 Made by: Liangyu Zhang Received: 12/02/15 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1209 Species: Caenorhabditis elegans Genotype: ieSi61 II; unc-119(ed3) III. Description: ieSi61 [ges-1p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Single copy transgene inserted into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in the intestine. This strain can be used for auxin-inducible degradation (AID) in the intestine. Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Mutagen: Outcrossed: x0 Made by: Liangyu Zhang Received: 12/02/15 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1210 Species: Caenorhabditis elegans Genotype: dhc-1(ie28[dhc-1::degron::GFP]) I; ieSi57 II. Description: ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Single copy transgene inserted into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in the soma. A degron::GFP tag was inserted at the 3' end of the endogenous dhc-1 coding sequence via CRISPR/Cas9. This strain can be used to examine spatial and temporal requirements for dynein in somatic tissue, and to serve as a control strain for auxin-inducible degradation (AID). Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Mutagen: Outcrossed: x0 Made by: Liangyu Zhang Received: 12/02/15 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1212 Species: Caenorhabditis elegans Genotype: dhc-1(ie28[dhc-1::degron::GFP]) I; ieSi60 II. Description: ieSi60 [myo-2p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Single copy transgene inserted into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in pharyngeal muscle. A degron::GFP tag was inserted at the 3' end of the endogenous dhc-1 coding sequence via CRISPR/Cas9. This strain can be used to examine spatial and temporal requirements for dynein in pharyngeal muscle, and to serve as a control strain for auxin-inducible degradation (AID). Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Mutagen: Outcrossed: x0 Made by: Liangyu Zhang Received: 12/02/15 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1213 Species: Caenorhabditis elegans Genotype: dhc-1(ie28[dhc-1::degron::GFP]) I; ieSi61 II. Description: ieSi61 [ges-1p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. A degron::GFP tag was inserted at the 3' end of the endogenous dhc-1 coding sequence via CRISPR/Cas9. Single copy transgene inserted into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in the intestine. This strain can be used to examine spatial and temporal requirements for dynein in the intestine, and to serve as a control strain for auxin-inducible degradation (AID). Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Mutagen: Outcrossed: x0 Made by: Liangyu Zhang Received: 12/02/15 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1215 Species: Caenorhabditis elegans Genotype: dhc-1(ie28[dhc-1::degron::GFP]) I; ieSi38 IV. Description: ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. Single copy transgene inserted into chromosome IV (cxTi10882) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in germ line and early embryos. A degron::GFP tag was inserted at the 3' end of the endogenous dhc-1 coding sequence via CRISPR/Cas9. This strain can be used to examine spatial and temporal requirements for dynein in the germ line and early embryos, and to serve as a control strain for auxin-inducible degradation (AID). Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Mutagen: Outcrossed: x0 Made by: Liangyu Zhang Received: 09/19/16 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1216 Species: C. elegans Genotype: air-2(ie31[degron::GFP::air-2]) I. Description: Degron and GFP tag inserted into endogenous air-2 gene locus by CRISPR/Cas9 engineering. Reference: Divekar NS, et al. PLoS Genet. 2021 May 20;17(5):e1009567. PMID: 34014923 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Liangyu Zhang Received: 10/19/21 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1217 Species: C.elegans Genotype: air-2(ie31[degron::gfp::air-2]) I; ieSi38 IV. Description: ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. Degron and GFP tag inserted into endogenous air-2 gene locus by CRISPR/Cas9 engineering allows auxin-inducible degradation (AID) of AIR-2 in germ line and early embryos. Reference: Divekar NS, et al. PLoS Genet. 2021 May 20;17(5):e1009567. PMID: 34014923 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Liangyu Zhang Received: 10/19/21 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1218 Species: Caenorhabditis elegans Genotype: syp-3(ok758) I; ieSi11 II; unc-119(ed3) III. Description: ieSi11 [syp-3p::EmeraldGFP::syp-3::syp-3 3'UTR + Cbr-unc-119(+)] II. ieSi11 was inserted into ttTi5605 II using MosSCI. Expression of GFP::SYP-3 largely complements syp-3(ok758), but some meiotic nondisjunction is detected above the N2 background (85% embryonic viability; ~1% male self-progeny;). GFP::SYP-3 expression is readily detected in spermatocytes and oocytes in the germline, and localizes to the interface between paired homologous chromosomes during most of meiotic prophase. Reference: Rog O, Dernburg AF. Cell Rep. 2015 Mar 10. pii: S2211-1247(15)00178-3. Mutagen: MosSCI Outcrossed: x>3 Made by: Ofer Rog Received: 05/13/16 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1219 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; ieSi21 IV. Description: ieSi21 [sun-1p::sun-1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. ieSi21 was inserted into cxTi10882 IV using MosSCI. Expression of the transgenic SUN-1::mRuby fusion protein complements the sun-1 deletion allele. SUN-1::mRuby is expressed throughout the germline and in the early embryo, where it localizes to nuclear envelope and associates with chromosome pairing centers during early meiotic prophase. Reference: Rog O, Dernburg AF. Cell Rep. 2015 Mar 10. pii: S2211-1247(15)00178-3. Mutagen: MosSCI Outcrossed: x>3 Made by: Ofer Rog Received: 05/13/16 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1230 Species: Caenorhabditis elegans Genotype: htp-3(tm3655) I; ieSi6 II; unc-119(ed3) III. Description: ieSi6 [htp-3p::htp-3::GFP + Cbr-unc-119(+)] II. Maintain at 20C. Although silencing of the transgene has not observed, it may be helpful to maintain it over htp-3(tm3655) to continually select for expression. unc-119(ed3) might not be homozygous in this strain. Reference: Kim et al. Dev Cell. 2015 Oct 26;35(2):247-61. Mutagen: MosSCI Outcrossed: x3 Made by: Ofer Rog Received: 06/24/16 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1319 Species: Caenorhabditis elegans Genotype: plk-2(ok1936) I; ieSi21 IV; sun-1(ok1282) V. Description: ieSi21 [sun-1::mRuby] IV. Homozygous animals developed normally, their self-progeny showed reduced viability, and many survivors were males (8%). Mutagen: Outcrossed: x0 Made by: Ofer Rog Received: 11/07/19 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1352 Species: Caenorhabditis elegans Genotype: ieSi64 II; unc-119(ed3) III. Description: ieSi64 [gld-1p::TIR1::mRuby::gld-1 3’UTR + Cbr-unc-119(+)] II. Single copy transgene inserted into chromosome II (oxTi179) expressing a modified Arabidopsis thaliana TIR1 tagged with mRuby in the germ line and early embryos. Comparing to CA1472, this strain expresses a higher level of TIR1 and can induce a faster degradation of AID-tagged proteins in the germ line and early embryos. Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Mutagen: Outcrossed: x0 Made by: Liangyu Zhang Received: 01/29/18 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1353 Species: Caenorhabditis elegans Genotype: ieSi65 II; unc-119(ed3) III. Description: ieSi65 [sun-1p::TIR1::sun-1 3’UTR + Cbr-unc-119(+)] II. Single copy transgene inserted into chromosome II (oxTi179) expressing a modified Arabidopsis thaliana TIR1 in germ line and early embryos. This strain can be used for auxin-inducible degradation (AID) in germ line and early embryos. Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Mutagen: Outcrossed: x0 Made by: Liangyu Zhang Received: 01/29/18 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1369 Species: Caenorhabditis elegans Genotype: zhp-1(ie62[zhp-1::AID::3xFLAG]) I; meIs8 II; spo-11(ie59[spo-11::AID::3xFLAG]) ieSi38 IV. Description: meIs8 [pie-1p::GFP::cosa-1 + unc-119(+)] II. ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. Single copy transgene inserted into chromosome IV (cxTi10882) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in germ line and early embryos. This strain can be used for auxin-inducible degradation (AID) in germ line and early embryos. References: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Zhang et al., Elife. 2018 Mar 9;7. pii: e30789. Mutagen: Outcrossed: x0 Made by: Liangyu Zhang Received: 11/07/19 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1377 Species: Caenorhabditis elegans Genotype: zhp-2(ie67[zhp-2::AID::3xFLAG]) I; meIs8 II; spo-11(ie60[spo-11::AID::3xFLAG]) ieSi38 IV. Description: meIs8 [pie-1p::GFP::cosa-1 + unc-119(+)] II. ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. Single copy transgene inserted into chromosome IV (cxTi10882) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in germ line and early embryos. This strain can be used for auxin-inducible degradation (AID) in germ line and early embryos. References: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Zhang et al., Elife. 2018 Mar 9;7. pii: e30789. Mutagen: Outcrossed: x0 Made by: Liangyu Zhang Received: 11/07/19 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1421 Species: Caenorhabditis elegans Genotype: meIs8 dsb-2(ie58[dsb-2::AID::3xFLAG]) II; ieSi38 IV. Description: meIs8 [pie-1p::GFP::cosa-1 + unc-119(+)] II. ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. Single copy transgene inserted into chromosome IV (cxTi10882) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in germ line and early embryos. This strain can be used for auxin-inducible degradation (AID) in germ line and early embryos. References: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Zhang et al., Elife. 2018 Mar 9;7. pii: e30789. Mutagen: Outcrossed: x0 Made by: Liangyu Zhang Received: 11/07/19 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1423 Species: Caenorhabditis elegans Genotype: meIs8 II; spo-11(ie59[spo-11::AID::3xFLAG]) ieSi38 IV. Description: meIs8 [pie-1p::GFP::cosa-1 + unc-119(+)] II. ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. Single copy transgene inserted into chromosome IV (cxTi10882) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in germ line and early embryos. This strain can be used for auxin-inducible degradation (AID) in germ line and early embryos. References: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Zhang et al., Elife. 2018 Mar 9;7. pii: e30789. Mutagen: Outcrossed: x0 Made by: Liangyu Zhang Received: 11/07/19 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA1472 Species: Caenorhabditis elegans Genotype: ieSi68 II; unc-119(ed3) III. Description: ieSi68 [sun-1p::TIR1::mRuby::htp-1 3'UTR + Cbr-unc-119(+)] II. Single copy transgene inserted into chromosome II (oxTi179) expressing a modified Arabidopsis thaliana TIR1 tagged with mRuby in the germ line and early embryos. This strain can be used for auxin-inducible degradation (AID) in germ line and early embryos. Reference: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Mutagen: Outcrossed: x0 Made by: Liangyu Zhang Received: 01/29/18 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA151 Species: Caenorhabditis elegans Genotype: him-8(me4) IV. Description: 40% XO self progeny. Recessive. Mutagen: EMS Outcrossed: x3 Made by: Anne Villeneuve Received: 01/26/06 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA257 Species: Caenorhabditis elegans Genotype: him-8(tm611) IV. Description: 37% XO self progeny. Recessive. Mutagen: Outcrossed: x3 Made by: Shohei Mitani Received: 01/26/06 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA258 Species: Caenorhabditis elegans Genotype: zim-2(tm574) IV. Description: Weak Him. Produces unhatched eggs. Mutagen: UV/TMP Outcrossed: x5 Made by: S. Mitani Received: 03/06/07 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA324 Species: Caenorhabditis elegans Genotype: zim-1(tm1813) IV. Description: Weak Him. Produces unhatched eggs. Mutagen: UV/TMP Outcrossed: x5 Made by: S. Mitani Received: 03/06/07 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA388 Species: Caenorhabditis elegans Genotype: pch-2(tm1458) II. Description: Defective for meiotic synapsis checkpoint. Recessive. Mutagen: Outcrossed: x3 Made by: Shohei Mitani Received: 01/26/06 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA448 Species: Caenorhabditis elegans Genotype: unc-24(e138) zim-3(tm2303) IV. Description: Unc. Weak Him. Produces unhatched eggs. Mutagen: UV/TMP Outcrossed: x5 Made by: Carolyn Phillips Received: 03/06/07 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA538 Species: Caenorhabditis elegans Genotype: rad-51(lg8701)/mIs11 IV. Description: mIs11 [myo-2p::GFP + pes-10p::GFP + F22B7.9::GFP]. Pick WT with dim GFP+ in pharynx to maintain. mIs11 homozygotes will quickly overtake the population if not selected against. GFP expression in 4-cell embryos, pharyngeal muscle and gut. Heterozygotes are WT with dim GFP signal in the pharynx. mIs11 homozygotes are WT with bright GFP in the pharynx. rad-51(lg8701) homozygotes are non-GFP and throw dead eggs. Mutagen: Outcrossed: x>8 Made by: Needhi Bhalla Received: 04/30/07 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA649 Species: Caenorhabditis elegans Genotype: ubc-9(tm2610) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Uncs, dead eggs, and Pvul slow growing tm2610 homozygotes. Pick Unc to maintain and check for correct segregation of progeny. Reference: Bhalla N, et al. PLoS Genet. 2008 4(10) e1000235. Mutagen: Outcrossed: x Made by: A. Dernburg Received: 03/12/13 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA756 Species: Caenorhabditis elegans Genotype: ieSi1 II; ltIs37 IV. Description: ieSi1 [htp-3p::GFP::him-8 + unc-119(+)] II. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. ieSi1 is prone to silencing; GFP might not be visible at lower magnifications. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Wynne DJ, et al. J Cell Biol. 2012 Jan 9;196(1):47-64. Mutagen: Outcrossed: x Made by: Dave Wynne Received: 03/15/25 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CA998 Species: Caenorhabditis elegans Genotype: ieDf2 [unc-119+]/mIs11 IV. Description: mIs11 [myo-2p::GFP + pes-10p::GFP + F22B7.9::GFP]. Heterozygotes are wild-type with dim GFP signal in the pharynx. mIs11 homozygotes are wild-type with bright GFP in the pharynx. ieDf2 homozygotes (non-GFP) develop normally but produce 97.5% inviable embryos and a high frequency of males among the surviving self-progeny. Pick WT with dim GFP+ in pharynx to maintain. mIs11 homozygotes will quickly overtake the population if not selected against. GFP expression in 4-cell embryos, pharyngeal muscle and gut. ieDf2 is a deficiency of zim-1, zim-2, zim-3, and him-8 generated by MosDel, resulting in single-copy insertion of a copy of the C. briggsae unc-119 gene on Chromosome IV. The deletion spans the sequences from the beginning of the zim-1 coding sequence through the ttTi22866 Mos1 insertion site. Mutagen: MosDel Outcrossed: x3 Made by: Regina Rillo Received: 10/22/13 Univ California, Berkeley CA, USA ----------------------------------------------------------------------------- Strain: CB1 Species: Caenorhabditis elegans Genotype: dpy-1(e1) III. Description: Dpy. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 09/30/79 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1001 Species: Caenorhabditis elegans Genotype: flu-3(e1001) II. Description: Increased gut fluorescence, purple. M-MATING++ 1-10%WT. Recessive. Mutagen: EMS Outcrossed: x Made by: Babu P Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1002 Species: Caenorhabditis elegans Genotype: flu-1(e1002) V. Description: Increased gut fluorescence, bluish purple. M-MATING++ 1-10%WT. Semi-dominant. Mutagen: EMS Outcrossed: x Made by: Babu P Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1003 Species: Caenorhabditis elegans Genotype: kynu-1(e1003) X. Description: Reduced gut fluorescence, dull green. M-MATING+++ 10-30%WT. Mutagen: EMS Outcrossed: x Made by: Babu P Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1004 Species: Caenorhabditis elegans Genotype: flu-4(e1004) X. Description: Increased gut fluorescence, blue. M-MATING++++ >30%WT. Mutagen: EMS Outcrossed: x Made by: Babu P Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1005 Species: Caenorhabditis elegans Genotype: unc-59(e1005) I. Description: Poor backward movement. Protrusive vulva. Gonad extrusion. Egg-laying defects. Recessive. Mutagen: Outcrossed: x Made by: Received: 03/01/01 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: CB1008 Species: Caenorhabditis elegans Genotype: unc-54(e1008) I. Description: Unc. Suppressed by sup-5 and sup-7. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 08/01/82 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: CB1009 Species: Caenorhabditis elegans Genotype: unc-54(e1009) I. Description: Paralyzed Unc. Null allele. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB101 Species: Caenorhabditis elegans Genotype: unc-9(e101) X. Description: Unc. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB1017 Species: Caenorhabditis elegans Genotype: vab-8(e1017) V. Description: Degenerate tail. Posterior half is thin, pale, uncoordinated. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 02/01/82 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB102 Species: Caenorhabditis elegans Genotype: unc-10(e102) X. Description: Unc. Weak coiler. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB1026 Species: Caenorhabditis elegans Genotype: lin-1(e1026) IV. Description: Multi-vulva (Muv). Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1033 Species: Caenorhabditis elegans Genotype: che-2(e1033) X. Description: Chemotaxis abnormal. Amphid defect-EM. M-MATING-NO SUCCESS Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1034 Species: Caenorhabditis elegans Genotype: che-1(e1034) fer-1(hc1) I. Description: Chemotaxis abnormal. Temperature sensitive fertilization defective. Maintain at 15C. M-MATING+++ 10-30%WT. Mutagen: EMS Outcrossed: x Made by: Lewis J Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1039 Species: Caenorhabditis elegans Genotype: unc-5(e53) IV; nuc-1(e1392) X. Description: Unc. DNAse undetectable. Gut DNA fluoresence abnormal. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB105 Species: Caenorhabditis elegans Genotype: unc-22(e105) IV. Description: Mild Twitcher Unc. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 08/01/82 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: CB1062 Species: Caenorhabditis elegans Genotype: daf-18(e1375) IV; vab-3(e1062) X. Description: Dauer defective. Notched head. Unlinked double. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1065 Species: Caenorhabditis elegans Genotype: him-2(e1065) I. Description: Segregates males. Recessive. M-MATING+++ 10-30%WT. Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1066 Species: Caenorhabditis elegans Genotype: mec-1(e1066) V. Description: Mechanosensory abnormal. Small. Lethargic. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Lewis J Received: 06/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1068 Species: Caenorhabditis elegans Genotype: unc-79(e1068) III. Description: Unc-fainter. Stops. Stiff anterior. Mutagen: EMS Outcrossed: x Made by: Lewis J Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1069 Species: Caenorhabditis elegans Genotype: unc-80(e1069) V. Description: Unc-stiff anterior. Mutagen: EMS Outcrossed: x Made by: Lewis J Received: 02/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1071 Species: Caenorhabditis elegans Genotype: mor-1(e1071) III. Description: Head abnormal. Movement almost wild-type. Mutagen: EMS Outcrossed: x Made by: Lewis J Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1072 Species: Caenorhabditis elegans Genotype: unc-29(e1072) I. Description: Very sluggish as L1, moves better as adult. Weak kinker. Head region stiff. Moves better in reverse, fairly active. Levamisole resistant. Mutagen: EMS Outcrossed: x Made by: Lewis J Received: 05/01/76 University of Texas, San Antonio ----------------------------------------------------------------------------- Strain: CB1073 Species: Caenorhabditis elegans Genotype: che-5(e1073) IV. Description: Unc. Chemotaxis abnormal. Short. Variable Dpyish. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Lewis J Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB108 Species: Caenorhabditis elegans Genotype: vab-5(e108) ?. Description: Notched head. M-MATING++++ >30%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB109 Species: Caenorhabditis elegans Genotype: unc-16(e109) III. Description: Unc-sluggish. Small. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB1091 Species: Caenorhabditis elegans Genotype: unc-13(e1091) I. Description: Unc-paralysed, kinky, small, irregular pharyngeal pumping. Suppressed by sup-5 and sup-7. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 08/01/82 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: CB1092 Species: Caenorhabditis elegans Genotype: unc-54(e1092) I. Description: Paralyzed Unc. Null allele. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1108 Species: Caenorhabditis elegans Genotype: unc-54(e1108) I. Description: Slow moving Unc. Mutagen: EMS Outcrossed: x Made by: Baillie D Received: 09/01/80 Baylor College of Medicine, Houston, TX ----------------------------------------------------------------------------- Strain: CB1111 Species: Caenorhabditis elegans Genotype: cat-1(e1111) X. Description: Catecholamine abnormal. Recessive. M-MATING++ 1-10%WT. Previously called ctl-2. Mutagen: EMS Outcrossed: x0 Made by: Sulston J Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1112 Species: Caenorhabditis elegans Genotype: cat-2(e1112) II. Description: Catecholamine absent. Recessive. M-MATING+++ 10-30%WT. See also WBPaper00003844. Mutagen: EMS Outcrossed: x Made by: Sulston J Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB112 Species: Caenorhabditis elegans Genotype: unc-20(e112) X. Description: Temperature sensitive. At 15C, L1 larvae are coilers, adults are wild-type. At 25C, severe kinker, some coiling. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB1122 Species: Caenorhabditis elegans Genotype: cccp-1(e1122) III. Description: Movement slow. Egg retainer. Recessive. M-MATING+++ 10-30%WT. Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1124 Species: Caenorhabditis elegans Genotype: che-3(e1124) I. Description: Chemotaxis abnormal. Crawls off plate. Dauer defective. Small. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: J. Hodgkin Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1125 Species: Caenorhabditis elegans Genotype: mor-2(e1125) IV. Description: Chemotaxis abnormal. Rounded head. Mutagen: EMS Outcrossed: x Made by: Lewis J Received: 02/01/82 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1126 Species: Caenorhabditis elegans Genotype: che-6(e1126) IV. Description: Chemotaxis defective: Cl-. Dauer recovery slow. Movement almost wild-type. Recessive. M-MATING+++ 10-30%WT. Mutagen: EMS Outcrossed: x Made by: Lewis J Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1128 Species: Caenorhabditis elegans Genotype: che-7(e1128) V. Description: Chemotaxis defective: Cl-. Eggs laid off bacteria. Small. Recessive. M-MATING+++ 10-30%WT. Mutagen: EMS Outcrossed: x Made by: Lewis J Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB113 Species: Caenorhabditis elegans Genotype: unc-17(e113) IV. Description: Unc, coiler. Lannate sensitive. Mutagen: EMS Outcrossed: x0 Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB1138 Species: Caenorhabditis elegans Genotype: him-6(e1104) IV. Description: Segregates males (5%). Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1141 Species: Caenorhabditis elegans Genotype: cat-4(e1141) V. Description: Catecholamine abnormal. Recessive. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Sulston J Received: 03/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1147 Species: Caenorhabditis elegans Genotype: him-3(e1147) IV. Description: 3% XO self progeny. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 01/01/79 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1157 Species: Caenorhabditis elegans Genotype: unc-54(e1157) I. Description: Temperature sensitive. Unc. Recessive. Dominant Slow. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 09/01/80 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1166 Species: Caenorhabditis elegans Genotype: dpy-4(e1166) IV. Description: Dpy. M-MATING+++ 10-30%WT. Weakly semi-dominant. Mutagen: ICR Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1168 Species: Caenorhabditis elegans Genotype: unc-54(e1168) I. Description: Paralyzed Unc. Null allele. Mutagen: ICR Outcrossed: x Made by: Baillie D Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1170 Species: Caenorhabditis elegans Genotype: unc-55(e1170) I. Description: Unc-slow, very poor backing, tends to coil. Healthy. Mutagen: ICR Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1172 Species: Caenorhabditis elegans Genotype: unc-24(e1172) IV. Description: Unc. Mutagen: ICR Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1174 Species: Caenorhabditis elegans Genotype: unc-84(e1174) X. Description: Migration defective: pre VC. Variable expressivity. Reverse kinker as adult. L1 moves well. Temperature sensitive. Can be maintained at 20C. Mutagen: ICR Outcrossed: x Made by: D. Riddle Received: 07/01/77 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1179 Species: Caenorhabditis elegans Genotype: unc-22(e1179) IV. Description: Twitcher Unc. Mutagen: ICR Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1180 Species: Caenorhabditis elegans Genotype: dpy-11(e1180) V. Description: Piggy phenotype (severe Dpy). Growth slow. Poor viability. Mutagen: ICR Outcrossed: x Made by: Received: 12/01/77 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1189 Species: Caenorhabditis elegans Genotype: unc-51(e1189) V. Description: Paralysed, Dpy, tends to curl. Egl. Mutagen: ICR Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1190 Species: Caenorhabditis elegans Genotype: unc-57(e1190) I. Description: Unc-kinky backing. Slow moving. Mutagen: ICR Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1193 Species: Caenorhabditis elegans Genotype: unc-33(e1193) IV. Description: Unc-almost paralyzed. Growth slow. Not revertible DES. Mutagen: ICR Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1196 Species: Caenorhabditis elegans Genotype: unc-26(e1196) IV. Description: Unc-severe kinker. Small. Scrawny. Flaccid. Little movement. Slow pharyngeal pumping. Mutagen: ICR Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1197 Species: Caenorhabditis elegans Genotype: unc-44(e1197) IV. Description: Coiler Unc. Growth slow. Mutagen: ICR Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1199 Species: Caenorhabditis elegans Genotype: unc-41(e1199) V. Description: Kinky Unc. Growth slow. Mutagen: ICR Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB12 Species: Caenorhabditis elegans Genotype: dpy-9(e12) IV. Description: Dpy. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB120 Species: Caenorhabditis elegans Genotype: unc-4(e120) II. Description: Uncoordinated. May contain a weak daf-2 mutation (sa875). See Ailion and Thomas in Genetics 165: 127-144 2003. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB1201 Species: Caenorhabditis elegans Genotype: unc-54(e1201) I. Description: Paralyzed Unc. Null allele. Mutagen: ICR Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1214 Species: Caenorhabditis elegans Genotype: unc-15(e1214) I. Description: Limp paralyzed Unc. Larvae move slightly better. Egl. Disorganized muscle structure. Suppressed by sup-5 and sup-7. Mutagen: EMS Outcrossed: x Made by: Waterston R Received: 06/01/80 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: CB1215 Species: Caenorhabditis elegans Genotype: unc-15(e1215) I. Description: Slow moving Unc. Paramyosin abnormal. Mutagen: EMS Outcrossed: x Made by: Waterston R Received: 01/01/80 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: CB1216 Species: Caenorhabditis elegans Genotype: unc-87(e1216) I. Description: Body muscle abnormal. Limp paralyzed or very sluggish; somewhat Egl; larvae more paralyzed. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Waterston R Received: 02/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1217 Species: Caenorhabditis elegans Genotype: unc-78(e1217) X. Description: Slow moving Unc. Body Muscle abnormal. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Waterston R Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1220 Species: Caenorhabditis elegans Genotype: unc-82(e1220) IV. Description: Slightly slow Unc. Movement almost wild-type. Body muscle abnormal. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Waterston R Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1253 Species: Caenorhabditis elegans Genotype: che-3(e1253) I. Description: Chemosensory abnormal. Slightly small. Defective dauer formation. M-MATING NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1255 Species: Caenorhabditis elegans Genotype: vab-11(e1255) IV. Description: Blebs on tail of adult hermaphrodite. Tail irregular, tail cuticle sometimes separated, tail spike sometimes truncated. Some animals have enlarged excretory canals. Mutagen: EMS Outcrossed: x1 Made by: J. Hodgkin Received: 01/21/97 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1256 Species: Caenorhabditis elegans Genotype: him-3(e1256) IV. Description: Throws 11% males. Mutagen: Outcrossed: x Made by: Received: 03/15/25 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: CB1258 Species: Caenorhabditis elegans Genotype: unc-54(e1258) I. Description: Paralyzed Unc. Null allele. Mutagen: DES Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1259 Species: Caenorhabditis elegans Genotype: dpy-19(e1259) III. Description: Temperature sensitive Dpy. Reproduction poor at 25C. M-MATING+ AT LOW TEMPERATURE ONLY. Mutagen: DES Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1260 Species: Caenorhabditis elegans Genotype: unc-44(e1260) IV. Description: Paralyzed Unc. Small. Mutagen: DES Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1261 Species: Caenorhabditis elegans Genotype: unc-33(e1261) IV. Description: Paralyzed Unc. Mutagen: DES Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1265 Species: Caenorhabditis elegans Genotype: unc-104(e1265) II. Description: Unc. Slow moving. Mutagen: Outcrossed: x Made by: Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB1266 Species: Caenorhabditis elegans Genotype: him-4(e1266) X. Description: 6% XO self-progeny. Males sterile. M-MATING-NO SUCCESS. Mutagen: ICR Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1267 Species: Caenorhabditis elegans Genotype: him-4(e1267) X. Description: Male gonad abnormal. Males sterile. 6% XO self-progeny. M-MATING-NO SUCCESS. Mutagen: ICR Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1272 Species: Caenorhabditis elegans Genotype: unc-80(e1272) V. Description: Unc-fainter: stops. M-MATING+++ 10-30%WT. Mutagen: EMS Outcrossed: x Made by: Lewis J Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1275 Species: Caenorhabditis elegans Genotype: lin-1(e1275) IV. Description: Temperature sensitive. Lineage abnormal. Multi-vulva. M-MATING+POOR <1%WT. Mutagen: EMS Outcrossed: x Made by: Horvitz B Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB128 Species: Caenorhabditis elegans Genotype: dpy-10(e128) II. Description: Small Dpy. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB1281 Species: Caenorhabditis elegans Genotype: dpy-8(e1281) X. Description: Dpy. Temperature sensitive. Mutagen: EMS Outcrossed: x Made by: Edgar B Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1282 Species: Caenorhabditis elegans Genotype: dpy-20(e1282) IV. Description: Dpy. Temperature sensitive. Lethal cold sensitive. Male lethal. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Edgar B Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1291 Species: Caenorhabditis elegans Genotype: unc-79(e1291) III. Description: Unc-fainter: stops. Mutagen: ICR Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1292 Species: Caenorhabditis elegans Genotype: mec-1(e1292) V. Description: Touch insensitive; lethargic. Mutagen: ICR Outcrossed: x Made by: Don Riddle Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1295 Species: Caenorhabditis elegans Genotype: dpy-17(e1295) III. Description: Medium Dpy. Mutagen: ICR Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB130 Species: Caenorhabditis elegans Genotype: dpy-8(e130) X. Description: Medium Dpy. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB1300 Species: Caenorhabditis elegans Genotype: unc-54(e1300) I. Description: Unc. Suppressed by sup-5 and sup-7. Null allele? Milder than most null. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 08/01/82 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: CB1301 Species: Caenorhabditis elegans Genotype: unc-54(e1301) I. Description: Temperature sensitive Unc. Mutagen: EMS Outcrossed: x Made by: Received: 08/01/80 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: CB1309 Species: Caenorhabditis elegans Genotype: lin-2(e1309) X. Description: Vulvaless. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Horvitz B Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1313 Species: Caenorhabditis elegans Genotype: egl-17(e1313) X. Description: Egg-laying defective. Moderate to severe bloating. 30% make bags of worms. Males mate. [NOTE: Probably contains a tightly linked mutation which cannot be crossed off. Use MT3188 as reference strain. Michael Stern 6\97] Mutagen: EMS Outcrossed: x1 Made by: Horvitz B Received: 11/01/83 MIT, Cambridge MA, USA ----------------------------------------------------------------------------- Strain: CB1314 Species: Caenorhabditis elegans Genotype: dpy-19(e1314) III. Description: Temperature sensitive Dpy. Mutagen: Outcrossed: x Made by: Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB1315 Species: Caenorhabditis elegans Genotype: unc-54(e1315) I. Description: Paralyzed Unc. Severe phenotype. Recessive. Null allele. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1322 Species: Caenorhabditis elegans Genotype: lin-8(n111) II; lin-9(n112) III. Description: Multivulva. Mutagen: EMS Outcrossed: x Made by: Horvitz B Received: 12/01/80 MIT, Cambridge MA, USA ----------------------------------------------------------------------------- Strain: CB1323 Species: Caenorhabditis elegans Genotype: unc-82(e1323) IV. Description: Slow but normal development, disorganized muscle structure, somewhat Egl. Mutagen: EMS Outcrossed: x Made by: Robert Waterston Received: 10/10/03 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: CB1324 Species: Caenorhabditis elegans Genotype: dpy-7(e1324) X. Description: Dpy. Roller left hand. Temperature sensitive. Mutagen: EMS Outcrossed: x Made by: Edgar B Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1338 Species: Caenorhabditis elegans Genotype: mec-3(e1338) IV. Description: Mechanosensory abnormal. Neurons/Touch abnormal. Lethargic. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Sulston J Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1339 Species: Caenorhabditis elegans Genotype: mec-4(e1339) X. Description: Mechanosensory abnormal. Scored with difficulty. Sometimes sensitive in the tail. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Sulston J Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1340 Species: Caenorhabditis elegans Genotype: mec-5(e1340) X. Description: Mechanorsensory abnormal. Touch insensitive. Lethargic. M-MATING+++ 10-30%WT. Mutagen: EMS Outcrossed: x Made by: Sulston J Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1345 Species: Caenorhabditis elegans Genotype: dpy-17(e1345) III. Description: Dpy. Temperature sensitive. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1350 Species: Caenorhabditis elegans Genotype: sqt-1(e1350) II. Description: Dpy. Heterozygotes Roll. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1359 Species: Caenorhabditis elegans Genotype: dpy-2(e1359) II. Description: Roller. Temperature sensitive. Mutagen: DES Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1364 Species: Caenorhabditis elegans Genotype: daf-4(e1364) III. Description: Temperature sensitive dauer constitutive. 100% dauers at 25C. Dauer recovery poor at 15C. Small. M-Mating--no success. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1370 Species: Caenorhabditis elegans Genotype: daf-2(e1370) III. Description: Temperature sensitive dauer constitutive. Maintain at 15C. 100% dauers at 25C. 15% dauer formation at 20C. Long-lived. M-MATING+LOW TEMPERATURE ONLY. Mutagen: EMS Outcrossed: x Made by: Edgar B Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1372 Species: Caenorhabditis elegans Genotype: daf-7(e1372) III. Description: Temperature sensitive dauer constitutive. Crowds. 100% dauers at 25C. Leaky at 20C. Growth slow. M-MATING+LOW TEMPERATURE ONLY. Mutagen: EMS Outcrossed: x Made by: Edgar B Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1375 Species: Caenorhabditis elegans Genotype: daf-18(e1375) IV. Description: Dauer defective. Non-crowder. Chemotaxis normal. Mutagen: EMS Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1376 Species: Caenorhabditis elegans Genotype: daf-3(e1376) X. Description: Dauer defective. Non-crowder. M-MATING++++ >30%WT. Mutagen: EMS Outcrossed: x Made by: Lewis J Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1377 Species: Caenorhabditis elegans Genotype: daf-6(e1377) X. Description: Dauer defective. Crowds. Leaky. Dauer recovery normal. Chemotaxis defective-Na+. M-MATING++++ >30%WT. Mutagen: EMS Outcrossed: x Made by: Lewis J Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1378 Species: Caenorhabditis elegans Genotype: che-3(e1378) I. Description: Dauer defective. Not leaky. Crowds. Amphid defect by EM. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1379 Species: Caenorhabditis elegans Genotype: che-3(e1379) I. Description: Dauer defective. Crowds. Chemotaxis defective-Na+. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB138 Species: Caenorhabditis elegans Genotype: unc-24(e138) IV. Description: Weak kinker. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB1386 Species: Caenorhabditis elegans Genotype: daf-5(e1386) II. Description: Dauer defective. WT phenotype. M-MATING+++ 10-30%WT. Mutagen: EMS Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1387 Species: Caenorhabditis elegans Genotype: daf-10(e1387) IV. Description: Amphid defect. Chemotaxis defective (Na+). Crowds. Dauer defective. Very leaky. M-MATING+POOR <1%WT. Mutagen: EMS Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB139 Species: Caenorhabditis elegans Genotype: unc-7(e139) X. Description: Unc. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB1390 Species: Caenorhabditis elegans Genotype: eDp22 V. Description: Suppressor weak. eDp22 = sup-3(e1390). Mutagen: EMS Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1392 Species: Caenorhabditis elegans Genotype: nuc-1(e1392) X. Description: DNAse undetectable. M-MATING++++ >30%WT. Mutagen: EMS Outcrossed: x Made by: Received: 02/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1393 Species: Caenorhabditis elegans Genotype: daf-8(e1393) I. Description: Dauer constitutive. Temperature sensitive. Crowds. Chemotaxis normal. Grow at 15C. Mutagen: Outcrossed: x Made by: Edgar B Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1396 Species: Caenorhabditis elegans Genotype: mut-1(e1396). Description: Mutator. Mutagen: Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB14 Species: Caenorhabditis elegans Genotype: dpy-6(e14) X. Description: Dpy. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB1402 Species: Caenorhabditis elegans Genotype: unc-15(e1402) I. Description: Temperature sensitive Unc-paralyzed. WT at 15C. leaky at 25C. Sterile at 25C. Mutagen: EMS Outcrossed: x Made by: Edgar B Received: 10/01/80 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1407 Species: Caenorhabditis elegans Genotype: eDp23 V. Description: Mutagen: EMS Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1408 Species: Caenorhabditis elegans Genotype: unc-83(e1408) V. Description: Marginally Unc-cannot back. Lethargic. Migration pre VC defect. M-MATING+POOR <1%WT. Mutagen: EMS Outcrossed: x Made by: Horvitz B Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1410 Species: Caenorhabditis elegans Genotype: unc-84(e1410) X. Description: Marginally Unc-kinky backing. Temperature sensitive. Migration pre VC defect. M-MATING+POOR <1%WT. Can be maintained at 20C. Mutagen: EMS Outcrossed: x Made by: Horvitz B Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1413 Species: Caenorhabditis elegans Genotype: lin-7(e1413) II. Description: Vulvaless. Incomplete penetrance. Recessive. Males can mate. Mutagen: EMS Outcrossed: x Made by: Sulston J Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1414 Species: Caenorhabditis elegans Genotype: unc-85(e1414) II. Description: Bursae abnormal. Lineage abnormal. Unc-kinky backing. Unc-lurches forward. Egl. M-MATING-NO SUCCESS. Rec'd new stock from J. Hodgkin 12/4/00. Mutagen: EMS Outcrossed: x Made by: Horvitz B Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1416 Species: Caenorhabditis elegans Genotype: unc-86(e1416) III. Description: Egg-laying deficient. Lineage abnormal. Mechanosensory abnormal. M-MATING++ 1-10%WT. Serotonin def. Mutagen: EMS Outcrossed: x Made by: Horvitz B Received: 04/01/79 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1417 Species: Caenorhabditis elegans Genotype: lin-3(e1417) IV. Description: Vulvaless. Recessive. M-MATING+++ 10-30%WT. Mutagen: EMS Outcrossed: x Made by: Horvitz B Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1419 Species: Caenorhabditis elegans Genotype: unc-54(e1419) I. Description: Unc. Suppressed by sup-5 and sup-7. Mutagen: EMS Outcrossed: x Made by: Horvitz B Received: 08/01/82 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: CB1421 Species: Caenorhabditis elegans Genotype: unc-52(e1421) II. Description: Dystrophic body muscle. Weak allele. Mutagen: EMS Outcrossed: x Made by: Horvitz B Received: 08/01/82 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: CB1439 Species: Caenorhabditis elegans Genotype: lin-10(e1439) I. Description: Vulvaless. Mutagen: EMS Outcrossed: x Made by: Horvitz B Received: 12/01/80 MIT, Cambridge MA, USA ----------------------------------------------------------------------------- Strain: CB1449 Species: Caenorhabditis elegans Genotype: lin-7(e1449) II. Description: Vul. Mutagen: Outcrossed: x Made by: Bob Horvitz Received: 06/10/04 MIT, Cambridge MA, USA ----------------------------------------------------------------------------- Strain: CB1456 Species: Caenorhabditis elegans Genotype: lin-17(e1456) I. Description: Mutagen: EMS Outcrossed: x1 Made by: John Sulston Received: 12/16/96 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CB1459 Species: Caenorhabditis elegans Genotype: unc-87(e1459) I. Description: Unc. Very slow movement. Adults paralyzed. Eggs laid. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 06/01/78 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: CB1460 Species: Caenorhabditis elegans Genotype: unc-89(e1460) I. Description: Body muscle abnormal. Movement almost WT. Slightly small. M-MATING+++ 10-30%WT. Mutagen: EMS Outcrossed: x Made by: Waterston R Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1464 Species: Caenorhabditis elegans Genotype: sup-5(e1464) III. Description: Suppressor. Null allele. Sterile at 15C. Grow between 22.5 and 24C. Mutagen: EMS Outcrossed: x Made by: Waterston R Received: 01/01/78 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: CB1467 Species: Caenorhabditis elegans Genotype: him-5(e1467) V. Description: 20% XO self-progeny at 16C. Recessive. Temperature sensitive. M-MATING++++ >30%WT. Mutagen: EMS Outcrossed: x Made by: Horvitz B Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1472 Species: Caenorhabditis elegans Genotype: mec-6(e1342) I. Description: Mechanosensory abnormal. Touch insensitive. Lethargic. Mutagen: EMS Outcrossed: x Made by: Sulston J Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1477 Species: Caenorhabditis elegans Genotype: mec-7(e1343) X. Description: Mechanosensory abnormal - semidominant. Lethargic at 25C. Neurons/Touch abnormal. Mutagen: EMS Outcrossed: x Made by: Sulston J Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1479 Species: Caenorhabditis elegans Genotype: him-6(e1423) IV. Description: 15% XO self-progeny. 6% XXX self-progeny. Low brood size. 78% unhatched eggs. General non-disjunction. Recessive. Mutagen: EMS Outcrossed: x Made by: Horvitz B Received: 08/01/80 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1480 Species: Caenorhabditis elegans Genotype: him-7(e1480) V. Description: 3% XO self-progeny. Recessive.M-MATING+++ 10-30%WT. Mutagen: EMS Outcrossed: x Made by: Horvitz B Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1482 Species: Caenorhabditis elegans Genotype: sma-6(e1482) II. Description: Short adult and late larvae. Mutagen: EMS Outcrossed: x Made by: H. R. Horvitz Received: 08/11/87 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1487 Species: Caenorhabditis elegans Genotype: xpf-1(e1487) II. Description: Recessive. M-MATING++++ >30%WT. Mutagen: Outcrossed: x Made by: Hodgkin J Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1489 Species: Caenorhabditis elegans Genotype: him-8(e1489) IV. Description: 37% XO self-progeny. 6% XXX self-progeny. Recessive. M-MATING++ 1-10%WT. [Background mutation that causes rays 8 and 9 to fuse.] Mutagen: EMS Outcrossed: x Made by: Horvitz B Received: 01/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1490 Species: Caenorhabditis elegans Genotype: ali-1(e1934) him-5(e1490) V. Description: Faint or invisible alae in L1. Segregates males. Mutagen: Outcrossed: x Made by: J. Hodgkin Received: 09/01/87 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1494 Species: Caenorhabditis elegans Genotype: mec-9(e1494) V. Description: Mechanosensory abnormal. Touch insensitive. Lethargic. Recessive. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Chalfie M Received: 03/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB15 Species: Caenorhabditis elegans Genotype: unc-8(e15) IV. Description: Unc. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB1503 Species: Caenorhabditis elegans Genotype: mec-5(e1503) X. Description: Mechanosensory abnormal. Temperature sensitive. Mec at 25C. WT at 15C. Mutagen: EMS Outcrossed: x Made by: Chalfie M Received: 02/01/82 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1507 Species: Caenorhabditis elegans Genotype: unc-86(e1507) III. Description: HSN-. Egl. Mec. Mutagen: EMS Outcrossed: x Made by: Marty Chalfie Received: 06/24/98 MIT, Cambridge MA, USA ----------------------------------------------------------------------------- Strain: CB151 Species: Caenorhabditis elegans Genotype: unc-3(e151) X. Description: Unc. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB1511 Species: Caenorhabditis elegans Genotype: him-10(e1511) III. Description: Temperature sensitive. 2% XO self-progeny at 15C, 12% at 20C, and 27% at 25C. M-MATING++++ >30%WT. Mutagen: Outcrossed: x Made by: Chalfie M Received: 04/01/80 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1515 Species: Caenorhabditis elegans Genotype: mec-10(e1515) X. Description: Touch insensitive. Lethargic. Mutagen: EMS Outcrossed: x Made by: M. Chalfie Received: 01/01/81 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1517 Species: Caenorhabditis elegans Genotype: eDf2 III; eDp6 (III;f). Description: DpyUnc. See also WBPaper0000498 and WBPaper00002306. Mutagen: acetaldehyde Outcrossed: x Made by: Hodgkin J Received: 11/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1518 Species: Caenorhabditis elegans Genotype: her-1(e1518) V. Description: XX animals are WT. XO animals are fertile hermaphrodites. Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB152 Species: Caenorhabditis elegans Genotype: unc-5(e152) IV. Description: Unc. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB155 Species: Caenorhabditis elegans Genotype: unc-27(e155) X. Description: Unc. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB156 Species: Caenorhabditis elegans Genotype: unc-25(e156) III. Description: Shrinker-contracts both dorsally and ventrally when prodded. Slow, poor backing. Slightly small. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB1561 Species: Caenorhabditis elegans Genotype: her-1(e1561) V. Description: WT appearance. XO fertile hermaphrodites. Temperature sensitive. Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1562 Species: Caenorhabditis elegans Genotype: vab-7(e1562) III. Description: Unc. Tail abnormalities-twisted or knobbed. Recessive. M-MATING-NO SUCCESS. Mutagen: Outcrossed: x Made by: Culotti J Received: 07/01/80 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1597 Species: Caenorhabditis elegans Genotype: unc-103(e1597) III. Description: Semidominant. Paralyzed Unc. Prone to spontaneous suppression. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 02/01/82 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1598 Species: Caenorhabditis elegans Genotype: unc-1(e1598) X. Description: Coiler Unc. Dominant. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 02/01/82 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1600 Species: Caenorhabditis elegans Genotype: tra-2(f70) II. Description: Transforms XX to males. Variable expression. Masculinized self-fertile hermaphrodite. tra-2(f70) was crossed into the N2 background to construct a dpy-10 tra-2 unc-4 triple mutant, then crossed to remove the flanking dpy and unc (Jonathan Hodgkin). Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1611 Species: Caenorhabditis elegans Genotype: mec-4(e1611) X. Description: Mechanosensory abnormal. Touch insensitive. Dominant. Cells become vacuolated and die. Mutagen: EMS Outcrossed: x Made by: M. Chalfie Received: 01/01/81 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB164 Species: Caenorhabditis elegans Genotype: dpy-17(e164) III. Description: Dpy. Larvae abnormal. M-MATING+++ 10-30%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB169 Species: Caenorhabditis elegans Genotype: unc-31(e169) IV. Description: Slow moving Unc. Recessive. M-MATING+POOR <1%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1747 Species: Caenorhabditis elegans Genotype: unc-13(e309) I; sup-6(st19)/+ II; daf-1(e1146) IV. Description: Heterozygotes are WT and segregate WT, UncDaf and Lethals. Suppressed Unc in hets. Suppressed Daf in hets. sup-6 is recessive lethal. Mutagen: Outcrossed: x Made by: S. Brenner Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB177 Species: Caenorhabditis elegans Genotype: unc-46(e177) V. Description: Unc. Recessive. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB182 Species: Caenorhabditis elegans Genotype: dpy-3(e182) X. Description: Dpy. Recessive. M-MATING+POOR <1%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB184 Species: Caenorhabditis elegans Genotype: dpy-13(e184) IV. Description: Semi-dominant Dpy. Mapping marker standard. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB185 Species: Caenorhabditis elegans Genotype: lon-1(e185) III. Description: Long. Abnormal bursae. Epistatic to Small. Recessive. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB187 Species: Caenorhabditis elegans Genotype: rol-6(e187) II. Description: Right hand Roller. Recessive. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB188 Species: Caenorhabditis elegans Genotype: dpy-14(e188) I. Description: Dpy. Abnormal larvae. Temperature sensitive: lethal at 25C. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 08/01/77 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB189 Species: Caenorhabditis elegans Genotype: unc-32(e189) III. Description: Coiler Unc. Severe. Recessive. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1893 Species: Caenorhabditis elegans Genotype: unc-17(e113) dpy-13(e184) IV. Description: DpyUnc. e184 is semi-dominant. Mutagen: Outcrossed: x Made by: Received: 10/23/00 MIT, Cambridge MA, USA ----------------------------------------------------------------------------- Strain: CB190 Species: Caenorhabditis elegans Genotype: unc-54(e190) I. Description: Semi-paralyzed Unc. Null allele. Recessive. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB1918 Species: Caenorhabditis elegans Genotype: lon-1(e185) unc-32(e189) III. Description: Long. Unc. Linked closely. Mutagen: Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB193 Species: Caenorhabditis elegans Genotype: unc-29(e193) I. Description: Unc. Levamisole resistant. Recessive. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 06/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB1951 Species: Caenorhabditis elegans Genotype: unc-42(e270) sma-1(e30) V. Description: Small. Unc. Linked closely. Mutagen: Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2 Species: Caenorhabditis elegans Genotype: vab-1(e2) II. Description: Notched head. Penetrance <70%. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB2030 Species: Caenorhabditis elegans Genotype: unc-62(e644) dpy-11(e224) V. Description: UncDpy. Mutagen: Outcrossed: x Made by: S. Brenner Received: 04/05/99 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB204 Species: Caenorhabditis elegans Genotype: unc-33(e204) IV. Description: Paralyzed Unc. Recessive. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB205 Species: Caenorhabditis elegans Genotype: unc-26(e205) IV. Description: Growth slow. Unc and Small. Revertible. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2053 Species: Caenorhabditis elegans Genotype: dpy-18(e364) unc-64(e246) III. Description: DpyUnc. Mutagen: Outcrossed: x Made by: S. Brenner Received: 04/05/99 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2065 Species: Caenorhabditis elegans Genotype: dpy-11(e224) unc-76(e911) V. Description: DpyUnc. Mutagen: Outcrossed: x Made by: Sulston J Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2067 Species: Caenorhabditis elegans Genotype: unc-73(e936) dpy-5(e61) I. Description: UncDpy. Mutagen: Outcrossed: x Made by: S. Brenner Received: 04/05/99 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB207 Species: Caenorhabditis elegans Genotype: dpy-11(e207) V. Description: Severe dumpy (piggy), grows poorly. Behaves as a null allele. Sequenced: early nonsense mutation R4opal. Mutagen: EMS Outcrossed: x1 Made by: S. Brenner Received: 05/23/02 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2070 Species: Caenorhabditis elegans Genotype: bli-1(e935) rol-1(e91) II. Description: Roller. Very few animals are Blistered. Mutagen: Outcrossed: x Made by: J. Hodgkin Received: 07/13/94 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2095 Species: Caenorhabditis elegans Genotype: dpy-1(e1) sup-5(e1877) III; unc-51(e369) V. Description: Dpy. Slightly Unc. Semi-dominant suppressor. Suppressed Unc between 22.5 and 24C. Mutagen: Outcrossed: x Made by: Brenner S Received: 02/01/82 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2108 Species: Caenorhabditis elegans Genotype: dpy-18(e364) sup-1(e995) III. Description: Dpyish/Suppressed. Variable expression. Mutagen: Outcrossed: x Made by: Received: 03/15/25 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB211 Species: Caenorhabditis elegans Genotype: lev-1(e211) IV. Description: Levamisole resistant. Recessive. Marginally Unc-No sin wave. WT adults. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB2110 Species: Caenorhabditis elegans Genotype: unc-17(e245) IV; sup-2(e997) X. Description: Suppressed Unc. Movement almost WT. Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 06/01/80 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2167 Species: Caenorhabditis elegans Genotype: dpy-5(e61) unc-13(e1091) I. Description: Dpy. Unc. Mutagen: Outcrossed: x Made by: Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB2194 Species: Caenorhabditis elegans Genotype: dpy-11(e224) eDp22 V. Description: Dpy. Mutagen: EMS Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2195 Species: Caenorhabditis elegans Genotype: dpy-19(e1259) unc-32(e189) III. Description: Dpy. Coiler Unc. Closely linked. Doesn't grow at 25C. Mutagen: Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2196 Species: Caenorhabditis elegans Genotype: daf-4(e1364) unc-32(e189) III. Description: Temperature sensitive dauer constitutive. Unc. Closely linked. Mutagen: Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2198 Species: Caenorhabditis elegans Genotype: daf-3(e1376) lon-2(e678) X. Description: Dauer defective. Long. Mutagen: Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2199 Species: Caenorhabditis elegans Genotype: lon-2(e678) daf-6(e1377) X. Description: Dauer defective. Long. Mutagen: Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2203 Species: Caenorhabditis elegans Genotype: unc-54(e190) I; dpy-11(e224) eDp22 V. Description: Dpy. Movement Slow. Unc partially suppressed. Mutagen: Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2204 Species: Caenorhabditis elegans Genotype: unc-54(e190) I; eDp22 V. Description: Movement slow. Unc partially suppressed. Mutagen: EMS Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2206 Species: Caenorhabditis elegans Genotype: lon-1(e185) daf-4(e1364) unc-32(e189) III. Description: Temperature sensitive dauer constitutive. Long. Unc. Linked closely. Mutagen: Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2213 Species: Caenorhabditis elegans Genotype: unc-15(e73) I; eDp22 V. Description: Movement slow. Unc partially suppressed. Mutagen: EMS Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2216 Species: Caenorhabditis elegans Genotype: dpy-5(e61) mec-6(e1342) I. Description: Dpy. Mechanosensory Abnormal. Mutagen: Spontaneous Outcrossed: x Made by: Sulston J Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2217 Species: Caenorhabditis elegans Genotype: lon-2(e678) mec-7(e1343) X. Description: Long. Mechanosensory abnormal. Semidominant Mec. Mutagen: Outcrossed: x Made by: Sulston J Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2220 Species: Caenorhabditis elegans Genotype: unc-15(e73) I; eDp23 V. Description: Movement almost WT. Unc suppressed. Mutagen: EMS Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2221 Species: Caenorhabditis elegans Genotype: unc-54(e1315) I; dpy-11(e224) eDp23 V. Description: Dpy. Movement Slow. Unc partially suppressed. Mutagen: Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2222 Species: Caenorhabditis elegans Genotype: unc-42(e270) unc-41(e268) V. Description: Growth slow. Severe Unc. Linked closely. Mutagen: Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2233 Species: Caenorhabditis elegans Genotype: unc-69(e587) dpy-18(e364) III. Description: UncDpy. Mutagen: Outcrossed: x Made by: S. Brenner Received: 04/05/99 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2238 Species: Caenorhabditis elegans Genotype: lon-2(e678) unc-84(e1410) X. Description: Lon. Unc. Mutagen: Outcrossed: x Made by: Bob Horvitz Received: 12/01/98 MIT, Cambridge MA, USA ----------------------------------------------------------------------------- Strain: CB224 Species: Caenorhabditis elegans Genotype: dpy-11(e224) V. Description: Dpy. Recessive. Mapping marker standard. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2261 Species: Caenorhabditis elegans Genotype: dpy-5(e61) unc-40(e1430) I. Description: DpyUnc. Mutagen: Outcrossed: x Made by: Bob Horvitz Received: 01/26/98 MIT, Cambridge MA, USA ----------------------------------------------------------------------------- Strain: CB228 Species: Caenorhabditis elegans Genotype: unc-61(e228) V. Description: Unc. Recessive. Abnormal bursae. Males abnormal. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 09/01/77 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2305 Species: Caenorhabditis elegans Genotype: lon-1(e185) sup-5(e1464) III. Description: Lon, slow-growing. Very poor growth at less than 20C. Mutagen: Outcrossed: x1 Made by: J. Hodgkin Received: 08/11/98 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2317 Species: Caenorhabditis elegans Genotype: tra-2(e1425) II; sup-5(e1464) III. Description: tra-2 suppressed. Viable hermaphrodite. See also CGC 397. Grow between 22.5C and 24C. Mutagen: Outcrossed: x Made by: Hodgkin J Received: 11/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB2318 Species: Caenorhabditis elegans Genotype: sup-5(e1464) III; tra-3(e1107) IV. Description: tra-3 suppressed. Viable hermaphrodite. See also CGC 397. Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 11/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB2337 Species: Caenorhabditis elegans Genotype: him-2(e1065) I; mab-3(e1240) II. Description: Lineage abnormal. Recessive. Segregates males. Males abnormal. Mutagen: Outcrossed: x Made by: Hodgkin J Received: 04/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB2338 Species: Caenorhabditis elegans Genotype: mab-6(e1249) II; him-5(e1490) V. Description: Segregates males. Males abnormal. Mutagen: Outcrossed: x Made by: Hodgkin J Received: 03/01/80 MIT, Cambridge MA, USA ----------------------------------------------------------------------------- Strain: CB234 Species: Caenorhabditis elegans Genotype: unc-18(e234) X. Description: Severe Unc-paralyzed. Growth good. Lannate resistant. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB239 Species: Caenorhabditis elegans Genotype: ric-1(e239) III. Description: Slightly Unc. Lannate resistant. Recessive. Larvae Unc. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB24 Species: Caenorhabditis elegans Genotype: sqt-3(e24) V. Description: ts Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB246 Species: Caenorhabditis elegans Genotype: unc-64(e246) III. Description: Lannate resistant. Recessive. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB25 Species: Caenorhabditis elegans Genotype: unc-23(e25) V. Description: Unc. Mutagen: EMS Outcrossed: x0 Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB251 Species: Caenorhabditis elegans Genotype: unc-36(e251) III. Description: Unc. Recessive. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 09/01/77 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB257 Species: Caenorhabditis elegans Genotype: unc-39(e257) V. Description: Unc-easily scored. Recessive. M-MATING+POOR <1%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2580 Species: Caenorhabditis elegans Genotype: tra-2(e1094)/dpy-10(e128) II. Description: Heterozygotes are WT and segregate WT, Dpy and males. Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 02/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB2590 Species: Caenorhabditis elegans Genotype: tra-1(e1099)/dpy-18(e1096) III. Description: Heterozygotes are wild-type, and segregate wild-type heterozygotes, fertile wild-type males (tra-1 homozygotes), and Dpy. Can recombine: pick individual WT hermaphrodites and check for correct segregation of progeny to maintain. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 09/30/79 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2597 Species: Caenorhabditis elegans Genotype: tra-2(e1098)/dpy-10(e128) unc-4(e120) II. Description: Heterozygotes are WT and segregate WT, DpyUnc and males. Maintain by picking WT hermaphrodites. Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 06/01/80 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB261 Species: Caenorhabditis elegans Genotype: unc-59(e261) I. Description: Unc. Weakly semi-dominant. Forward and backward movement poor. Bursae abnormal. Lineage abnormal. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 09/01/77 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2612 Species: Caenorhabditis elegans Genotype: tra-3(e1107)/dpy-4(e1166) IV. Description: Maternal effect tra-3. Heterozygotes are WT and segregate WT and Dpy. Homozygous tra-3 animals are WT hermaphrodites which segregate abnormal sterile males or intersex. Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB2619 Species: Caenorhabditis elegans Genotype: eDf1 eDp21/dpy-11(e224) V. Description: Heterozygotes are WT and segregate WT, Dpy and lethals which die in larval development. See also WBPaper00001202. Mutagen: EMS Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB262 Species: Caenorhabditis elegans Genotype: unc-37(e262) I. Description: Coiler Unc. Severe. Recessive. M-MATING+POOR <1%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2620 Species: Caenorhabditis elegans Genotype: daf-9(e1406)/lon-2(e678) X. Description: Heterozygotes are WT and segregate WT, lethal dauers (DAUER-LIKE LARVAE) and Lon. Can recombine; check for correct segregation of progeny to maintain. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2621 Species: Caenorhabditis elegans Genotype: unc-15(e73) I; eDf1 eDp21/sma-1(e30) V. Description: Heterozygotes are slow moving. Segregates paralysed small. eDf1 eDp21 homozygotes die in larval development. Mutagen: Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2627 Species: Caenorhabditis elegans Genotype: lin-4(e912)/dpy-10(e128) II. Description: Heterozygotes are WT and segregate WT, Dpy and Vulvaless. Maintain by picking WT. Mutagen: P32 Outcrossed: x Made by: Chalfie M Received: 03/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB268 Species: Caenorhabditis elegans Genotype: unc-41(e268) V. Description: Unc. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB27 Species: Caenorhabditis elegans Genotype: dpy-3(e27) X. Description: Dpy. Recessive. M-MATING+POOR <1%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB270 Species: Caenorhabditis elegans Genotype: unc-42(e270) V. Description: Unc. Recessive. Mapping marker standard. M-MATING+POOR <1%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB271 Species: Caenorhabditis elegans Genotype: unc-40(e271) I. Description: Unc. Small. Egg Retainer. Serotonin Def. Recessive. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB2744 Species: Caenorhabditis elegans Genotype: lin-5(e1348)/dpy-10(e128) II. Description: Heterozygotes are WT. Segregates Dpy. Segregates sterile Unc--Unc cannot back. Balanced well. Mutagen: Outcrossed: x Made by: Sulston J Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2754 Species: Caenorhabditis elegans Genotype: tra-2(e1095)/dpy-10(e128) unc-4(e120) II. Description: Heterozygotes are WT and segregate WT, DpyUnc and males. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB2769 Species: Caenorhabditis elegans Genotype: eDf3/eDf24 I. Description: Heterozygotes are WT and segregate WT, dead eggs, and larval lethal (eDf24 homozygotes). eDf24 = let(e2000). Mutagen: DEO Outcrossed: x Made by: Phil Anderson Received: 09/01/82 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: CB2770 Species: Caenorhabditis elegans Genotype: eDf4/eDf24 I. Description: Heterozygotes are WT and segregate WT, dead eggs, and larval lethals (eDf24 homozygotes). eDf24 = let(e2000). Mutagen: DEO Outcrossed: x Made by: Phil Anderson Received: 01/01/87 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: CB2771 Species: Caenorhabditis elegans Genotype: eDf5/eDf24 I. Description: Hets are WT and segregate WT, dead eggs, and larval lethals (eDf24 homozygotes). eDf24 = let(e2000). Mutagen: DEO Outcrossed: x Made by: Phil Anderson Received: 02/15/90 MIT, Cambridge MA, USA ----------------------------------------------------------------------------- Strain: CB2772 Species: Caenorhabditis elegans Genotype: eDf6/eDf24 I. Description: Hets are WT and segregate WT, dead eggs, and larval lethals (eDf24 homozygotes). eDf24 = let(e2000). Mutagen: DEO Outcrossed: x Made by: Phil Anderson Received: 09/01/82 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: CB2773 Species: Caenorhabditis elegans Genotype: eDf7/eDf24 I. Description: Hets are WT and segregate WT, dead eggs, and larval lethals (eDf24 homozygotes). eDf24 = let(e2000). Mutagen: DEO Outcrossed: x Made by: Phil Anderson Received: 01/01/87 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: CB2775 Species: Caenorhabditis elegans Genotype: eDf9/eDf24 I. Description: Hets are WT and segregate WT, dead eggs, and larval lethals (eDf24 homozygotes). eDf24 = let(e2000). Mutagen: DEO Outcrossed: x Made by: Phil Anderson Received: 01/01/87 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: CB2776 Species: Caenorhabditis elegans Genotype: eDf10/eDf24 I. Description: Hets are WT and segregate WT, dead eggs, and larval lethals (eDf24 homozygotes). eDf24 = let(e2000). Mutagen: DEO Outcrossed: x Made by: Phil Anderson Received: 01/01/87 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: CB2777 Species: Caenorhabditis elegans Genotype: eDf11/eDf24 I. Description: Hets are WT and segregate WT, dead eggs, and larval lethals (eDf24 homozygotes). eDf24 = let(e2000). Mutagen: DEO Outcrossed: x Made by: Phil Anderson Received: 01/01/87 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: CB2778 Species: Caenorhabditis elegans Genotype: eDf12/eDf24 I. Description: Hets are WT and segregate WT, dead eggs, and larval lethals (eDf24 homozygotes). eDf24 = let(e2000). Mutagen: DEO Outcrossed: x Made by: Phil Anderson Received: 01/01/87 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: CB2779 Species: Caenorhabditis elegans Genotype: let-201(e1716) unc-54(e1092)/eDf24 I. Description: Heterozygotes are WT. eDf24 homozygotes arrest as early larvae. eDf24 = let(e2000). Mutagen: EMS Outcrossed: x Made by: Phil Anderson Received: 01/01/86 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: CB2780 Species: Caenorhabditis elegans Genotype: let-203(e1717) unc-54(e1092)/eDf24 I. Description: Heterozygotes are WT. eDf24 homozygotes arrest as early larvae. eDf24 = let(e2000). Mutagen: EMS Outcrossed: x Made by: Phil Anderson Received: 01/01/86 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: CB2781 Species: Caenorhabditis elegans Genotype: unc-54(e1092) let-208(e1718)/eDf24 I. Description: Heterozygotes are WT. eDf24 homozygotes arrest as early larvae. eDf24 = let(e2000). Mutagen: EMS Outcrossed: x Made by: Phil Anderson Received: 03/01/86 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: CB2782 Species: Caenorhabditis elegans Genotype: let-204(e1719) unc-54(e1092)/eDf24 I. Description: Heterozygotes are WT. eDf24 homozygotes arrest as early larvae. eDf24 = let(e2000). Mutagen: EMS Outcrossed: x Made by: Phil Anderson Received: 12/11/92 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: CB2784 Species: Caenorhabditis elegans Genotype: let-202(e1720) unc-54(e1092)/eDf24 I. Description: Heterozygotes are WT. eDf24 homozygotes arrest as early larvae. eDf24 = let(e2000). Mutagen: EMS Outcrossed: x Made by: Phil Anderson Received: 01/01/86 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: CB2785 Species: Caenorhabditis elegans Genotype: let-206(e1721) unc-54(e1092)/eDf24 I. Description: Heterozygotes are WT. eDf24 homozygotes arrest as early larvae. eDf24 = let(e2000). Mutagen: EMS Outcrossed: x Made by: Phil Anderson Received: 03/01/86 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: CB2786 Species: Caenorhabditis elegans Genotype: let-205(e1722) unc-54(e1092)/eDf24 I. Description: Heterozygotes are WT. eDf24 homozygotes arrest as early larvae. eDf24 = let(e2000). Mutagen: EMS Outcrossed: x Made by: Phil Anderson Received: 01/01/86 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: CB2787 Species: Caenorhabditis elegans Genotype: let-207(e1723) unc-54(e1092)/eDf24 I. Description: Heterozygotes are WT. eDf24 homozygotes arrest as early larvae. eDf24 = let(e2000). Mutagen: EMS Outcrossed: x Made by: Phil Anderson Received: 03/01/86 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: CB2810 Species: Caenorhabditis elegans Genotype: tra-1(e1575)/+ III; unc-42(e270) him-5(e1490) dpy-21(e428) V. Description: Pick Unc hermaphrodite to maintain (these will be tra-1/+; unc-42 him-5 dpy-21 XO animals). Segregates Unc hermaphrodites (tra-1/+ III), Unc males (+/+ III), and DpyUnc hermaphrodites (+/+ III). dpy-21 not expressed in XO. dpy-21(e428) V; XX animals are weak Dpy. dpy-21(e428) V; XO animals are non-Dpy. Mutagen: Outcrossed: x Made by: Hodgkin J Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB2818 Species: Caenorhabditis elegans Genotype: eDf13/eDf24 I. Description: Heterozygotes are WT. eDf24 homozygotes arrest as early larvae. eDf24 = let(e2000). Mutagen: DEO Outcrossed: x Made by: Phil Anderson Received: 01/01/87 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: CB2819 Species: Caenorhabditis elegans Genotype: eDf14/eDf24 I. Description: Heterozygotes are WT. eDf24 homozygotes arrest as early larvae. eDf24 = let(e2000). Mutagen: DEO Outcrossed: x Made by: Phil Anderson Received: 01/01/87 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: CB2820 Species: Caenorhabditis elegans Genotype: eDf15/eDf24 I. Description: Heterozygotes are WT. eDf24 homozygotes arrest as early larvae. eDf24 = let(e2000). Mutagen: DEO Outcrossed: x Made by: Phil Anderson Received: 01/01/87 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: CB2821 Species: Caenorhabditis elegans Genotype: eDf16/eDf24 I. Description: Heterozygotes are WT. eDf24 homozygotes arrest as early larvae. eDf24 = let(e2000). Mutagen: DEO Outcrossed: x Made by: Phil Anderson Received: 01/01/87 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: CB2823 Species: Caenorhabditis elegans Genotype: tra-1(e1488) III; eDp6 (III;f). Description: Balanced well. Animals with the duplication are WT. Animals which have lost the duplication have a hermaphrodite gonad and intestine (they are self-fertile); the rest of the body is male. Mutagen: acetaldehyde Outcrossed: x Made by: Hodgkin J Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB2829 Species: Caenorhabditis elegans Genotype: tra-1(e1076) III; eDp6 (III;f). Description: Balanced well. Animals with the duplication are WT. Animals which have lost the duplication have a hermaphrodite gonad and intestine (they are self-fertile); the rest of the body is male. Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB2830 Species: Caenorhabditis elegans Genotype: tra-2(e1209)/dpy-10(e128) unc-4(e120) II. Description: Balances well. Transformer weak. Heterozygotes are WT and segregate WT, DpyUnc and males. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB2842 Species: Caenorhabditis elegans Genotype: unc-58(e665e2112) X. Description: Intragenic revertant of dominant mutation e665. Slight uncoordinated phenotype. Mutagen: EMS reversion mutagenesis Outcrossed: x1 Made by: S. Brenner Received: 09/30/92 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB286 Species: Caenorhabditis elegans Genotype: unc-45(e286) III. Description: Slow moving Unc. Body muscle abnormal. Temperature sensitive. Recessive. M-MATING+POOR <1%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB293 Species: Caenorhabditis elegans Genotype: unc-29(e293) I. Description: Levamisole resistant. Segregates Dpy. Mutagen: EMS Outcrossed: x Made by: Lewis J Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB2987 Species: Caenorhabditis elegans Genotype: unc-13(e309) I; dpy-10(e128) sup-6(st19)/dpy-10(e128) II. Description: Dominant suppressor of Unc. Dpy. sup-6 is recessive lethal. Heterozygotes are Dpy non-Unc. Pick Dpy non-Unc to maintain. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB30 Species: Caenorhabditis elegans Genotype: sma-1(e30) V. Description: Small. Recessive. Abnormal bursae. Males abnormal. M-MATING-NO SUCCESS. See also WBPaper00003721. Mutagen: EMS Outcrossed: x1 Made by: Brenner S Received: 10/01/85 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3019 Species: Caenorhabditis elegans Genotype: unc-54(e1258) I; eDf1 eDp21/+ V. Description: Movement slow. Suppressed Unc. Revertant. Heterozygotes move slowly and segregate larval lethals (eDf1 eDp21 homozygotes), and paralyzed Uncs. Mutagen: EMS Outcrossed: x0 Made by: Received: 03/15/25 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB3031 Species: Caenorhabditis elegans Genotype: unc-17(e245) IV; snb-1(e1563) V. Description: Dominant suppressor of Unc. Movement almost WT. Mutagen: UV Outcrossed: x Made by: Hodgkin J Received: 09/01/80 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3035 Species: Caenorhabditis elegans Genotype: unc-54(e1258) I; wdDp1 V. Description: Suppressed Unc. Mutagen: EMS Outcrossed: x Made by: Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB306 Species: Caenorhabditis elegans Genotype: unc-50(e306) III. Description: Levamisole resistant. Recessive. Kinky Unc. M-MATING+POOR <1%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3060 Species: Caenorhabditis elegans Genotype: sup-9(n180) II; unc-93(e1500) III. Description: Recessive Suppressor. XO Fertile hermaphrodite. WT phenotype. Mutagen: Spontaneous Outcrossed: x Made by: Horvitz B Received: 09/01/80 MIT, Cambridge MA, USA ----------------------------------------------------------------------------- Strain: CB307 Species: Caenorhabditis elegans Genotype: unc-47(e307) III. Description: Unc. Recessive. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB312 Species: Caenorhabditis elegans Genotype: unc-13(e312) I. Description: Slightly Unc. Suppressed by sup-5 and sup-7. Null allele? Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 08/01/82 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: CB315 Species: Caenorhabditis elegans Genotype: unc-34(e315) V. Description: Unc. Male spicules abnormal. Recessive. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 09/01/77 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3168 Species: Caenorhabditis elegans Genotype: him-1(e879) I; mab-3(e1240) II. Description: Lineage abnormal. Bursae abnormal. Segregates abnormal males. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 09/01/80 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB318 Species: Caenorhabditis elegans Genotype: unc-30(e318) IV. Description: Unc-cannot back. Recessive. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 04/01/78 Simon Fraser University, Vancouver, BC ----------------------------------------------------------------------------- Strain: CB3191 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Wild type, apparently identical to N2. Isolated by Carl Johnson in June 1973 in a garden in Altadena, CA. Sample obtained from laboratory of R.E. Russell in October 1980. Sent to CGC in Jan. 1997. Reference WBG 10(2) 140-141. Caenorhabditis elegans wild isolate. Mutagen: Outcrossed: x Made by: Carl Johnson Received: 01/21/97 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3192 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: WT. Resembles N2. Wild isolate from Altadena, CA. Caenorhabditis elegans wild isolate. Original name = GA2. Mutagen: Outcrossed: x0 Made by: C.D. Johnson Received: 01/12/00 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3193 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: WT. Resembles N2. Natural isolate from Altadena, CA. Caenorhabditis elegans wild isolate. Original name = GA3. Mutagen: Outcrossed: x0 Made by: C.D. Johnson Received: 01/12/00 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3194 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: WT. Resembles N2. Natural isolate from Altadena, CA. Caenorhabditis elegans wild isolate. Original name = GA4. Mutagen: Outcrossed: x0 Made by: C.D. Johnson Received: 01/12/00 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3195 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: WT. Resembles N2. Natural isolate from Altadena, CA. Caenorhabditis elegans wild isolate. Original name = GA5. Mutagen: Outcrossed: x0 Made by: C.D. Johnson Received: 01/12/00 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3196 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: WT. Resembles CB4854 (GA9). Natural isolate from Altadena, CA. Caenorhabditis elegans wild isolate. Original name = GA13. Mutagen: Outcrossed: x0 Made by: C.D. Johnson Received: 01/12/00 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3197 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: WT. Resembles CB4854 (GA9). Natural isolate from Altadena, CA. Caenorhabditis elegans wild isolate. Original name = GA23. Mutagen: Outcrossed: x0 Made by: C.D. Johnson Received: 01/12/00 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3198 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: WT. Resembles CB4853 (GA12). Natural isolate from Pasadena, CA. Caenorhabditis elegans wild isolate. Original name = PA2. Mutagen: Outcrossed: x0 Made by: E.M. Hedgecock Received: 01/12/00 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3199 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: WT. Resembles CB4857 (Cl2a). Natural isolate from Pasadena, CA. Caenorhabditis elegans wild isolate. Original name = PA3. Mutagen: Outcrossed: x0 Made by: E.M. Hedgecock Received: 01/12/00 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3202 Species: Caenorhabditis elegans Genotype: hch-1(e1734) X. Description: Delayed hatching from egg shell. QL and descendant cells migrate forward instead of backward (incomplete penetrance). Mutagen: Outcrossed: x Made by: Received: 03/15/25 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: CB3203 Species: Caenorhabditis elegans Genotype: ced-1(e1735) I. Description: Abnormal cell death. Mutagen: EMS Outcrossed: x1 Made by: Ed Hedgecock Received: 08/01/83 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3214 Species: Caenorhabditis elegans Genotype: smg-1(e1228) him-2(e1065) I. Description: Bursae abnormal. Have slightly protruding vulva in adults. Segregates males with abnormal swollen bursae. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 09/01/80 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3218 Species: Caenorhabditis elegans Genotype: him-2(e1065) I; mab-4(e1252) III. Description: Bursae abnormal. Vulva protruding. Segregates abnormal males. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 09/01/80 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3221 Species: Caenorhabditis elegans Genotype: him-2(e1065) I; mab-8(e1250) II. Description: Hermaphrodites are nearly WT. Vulva slightly swollen. Males have abnormal swollen bursae. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 02/01/82 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3234 Species: Caenorhabditis elegans Genotype: him-13(e1742) I. Description: Selfed hermaphrodites produce 5% males and 2% XXX hermaphrodites (percent of viable progeny), and 30% unhatched eggs (percent of total progeny). Mutagen: EMS Outcrossed: x2 Made by: Hedgecock/Hodgkin Received: 09/01/87 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB324 Species: Caenorhabditis elegans Genotype: unc-23(e324) V. Description: Unc. Head muscles degenerative. Recessive. M-MATING+POOR <1%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3241 Species: Caenorhabditis elegans Genotype: clr-1(e1745) II. Description: Temperature sensitive lethal: inviable at 25C, grows at 20C and 15C. Somewhat sickly. Small brood sizes. At 25C homozygotes have a starved translucent appearance, facilitating Nomarski visualization of neuron processes. Mutagen: EMS Outcrossed: x1 Made by: Ed Hedgecock Received: 06/01/85 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: CB3243 Species: Caenorhabditis elegans Genotype: rab-10(e1747) III. Description: Homozygous viable and looks almost normal by dissecting scope, but under Nomarski microscopy the gut has a very abnormal spongy appearance, which is especially noticeable in L4 larvae. Mutagen: Outcrossed: x Made by: Received: 09/20/95 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3252 Species: Caenorhabditis elegans Genotype: rnt-1(e1241) I; him-5(e1490) dpy-21(e428) V. Description: Hermaphrodites are Dpy and Him. Males are non-Dpy and Mab. Males have abnormal bursa and defective rays. Both sexes show lateral hypodermis lineage defexts. M-MATING+POOR <1%WT. Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 09/01/80 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3253 Species: Caenorhabditis elegans Genotype: dpy-23(e840) lon-2(e678) X. Description: Variably Dpy, slow growing. Inviable on MYOB medium. Mutagen: Outcrossed: x Made by: J. Hodgkin Received: 04/05/99 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3256 Species: Caenorhabditis elegans Genotype: mab-5(e1751) III. Description: Semidominant Unc(mild). Intragenic revertants common. Mutagen: EMS Outcrossed: xY Made by: Received: 10/13/92 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: CB3257 Species: Caenorhabditis elegans Genotype: ced-2(e1752) IV. Description: Abnormal cell death. Mutagen: EMS Outcrossed: x1 Made by: Ed Hedgecock Received: 08/01/83 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3261 Species: Caenorhabditis elegans Genotype: ced-1(e1754) I. Description: Mutagen: Outcrossed: x Made by: Ron Ellis Received: 03/23/07 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3270 Species: Caenorhabditis elegans Genotype: lon-2(e678) mec-7(e1506) X. Description: Long. Mechanosensory abnormal. Severe mechanosensory abnormal. Recessive. Mutagen: Outcrossed: x Made by: Chalfie M Received: 03/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB3271 Species: Caenorhabditis elegans Genotype: lon-2(e678) mec-10(e1515) X. Description: Long. Mechanosensory abnormal. Mutagen: Outcrossed: x Made by: Chalfie M Received: 03/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB3273 Species: Caenorhabditis elegans Genotype: lon-2(e678) mec-2(e75) X. Description: Long. Mechanosensory Abnormal. [NOTE: originally described as carrying e1084, this strain actually carries the e75 allele. The lesion has been verified by sequence analysis. (11/26/2018)] Mutagen: Outcrossed: x Made by: Chalfie M Received: 03/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB3274 Species: Caenorhabditis elegans Genotype: lon-2(e678) mec-4(e1497) X. Description: Long. Mechanosensory abnormal. Mutagen: EMS Outcrossed: x Made by: Chalfie M Received: 03/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB3275 Species: Caenorhabditis elegans Genotype: lon-2(e678) mec-5(e1504) X. Description: Long. Mechanosensory abnormal. Mutagen: EMS Outcrossed: x Made by: Chalfie M Received: 03/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB3276 Species: Caenorhabditis elegans Genotype: lon-2(e678) mec-7(e1527) X. Description: Dominant Mechanosensory Abnormal at 20C. Long. Mutagen: EMS Outcrossed: x Made by: Chalfie M Received: 03/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB3284 Species: Caenorhabditis elegans Genotype: mec-12(e1605) III. Description: Touch insensitive, lethargic. Mutagen: EMS Outcrossed: x Made by: Received: 01/01/81 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3297 Species: Caenorhabditis elegans Genotype: vab-9(e1744) II; him-5(e1490) V. Description: Him. M-MATING+POOR <1%WT. Tail abnormalities->knob-like swelling in larvae and adults. Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 06/01/80 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3298 Species: Caenorhabditis elegans Genotype: him-5(e1490) dpy-21(e428) V; mab-7(e1599) X. Description: Hermaphrodites are Dpy. Males are non-Dpy and have abnormal bursae in adult, with swollen rays. Males will mate, but with very low efficiency. [3/98: King Chow isolated a line from the CGC stock that was throwing 100% Mabs. Sent the strain back to the CGC to replace the old stock.] Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 01/01/80 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3299 Species: Caenorhabditis elegans Genotype: mab-5(e1239) III; him-5(e1490) dpy-21(e428) V. Description: Hermaphrodites are Dpy and segregate males. Males are non-Dpy and Mab. Both sexes show lineage alterations. Mutagen: Outcrossed: x Made by: Hodgkin J Received: 07/01/80 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB330 Species: Caenorhabditis elegans Genotype: unc-29(e330) I. Description: Unc. Recessive. Levamisole and lannate resistant. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 09/01/77 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3303 Species: Caenorhabditis elegans Genotype: mig-1(e1787) I. Description: QL migration reversed, HSN migration defective. Egl. Mutagen: EMS Outcrossed: x1 Made by: Ed Hedgecock Received: 03/01/87 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: CB3304 Species: Caenorhabditis elegans Genotype: vab-3(e1796) X. Description: Extra hypodermal nuclei. DTC migration abnormal. pka lin-20. Mutagen: EMS Outcrossed: x Made by: Ed Hedgecock Received: 07/29/97 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3313 Species: Caenorhabditis elegans Genotype: ect-2(e1778)/dpy-10(e128) II. Description: Poorly balanced. Hets are WT and segregate WT, Dpys and ect-2 homozygotes. ect-2 homozygotes are sterile Uncs which reach adulthood, sometimes giving polynucleate oocytes. ect-2 pka let-21. Mutagen: Outcrossed: x Made by: Ed Hedgecock Received: 09/03/96 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3323 Species: Caenorhabditis elegans Genotype: che-13(e1805) I. Description: No FITC uptake by phasmids and amphids. Dauer formation defective. Osmotic avoidance defective. Mutagen: EMS Outcrossed: x1 Made by: Ed Hedgecock Received: 06/01/85 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: CB3329 Species: Caenorhabditis elegans Genotype: che-10(e1809) II. Description: Defective osmotic avoidance. FITC-. Mutagen: EMS Outcrossed: x1 Made by: Ed Hedgecock Received: 06/01/85 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: CB3330 Species: Caenorhabditis elegans Genotype: che-11(e1810) V. Description: Defective in osmotic avoidance. FITC-. Dauer defective. Mutagen: EMS Outcrossed: x1 Made by: Ed Hedgecock Received: 06/01/85 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: CB3332 Species: Caenorhabditis elegans Genotype: che-12(e1812) V. Description: Defective osmotic avoidance. FITC uptake abnormal. Mutagen: EMS Outcrossed: x1 Made by: Ed Hedgecock Received: 06/01/85 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: CB3335 Species: Caenorhabditis elegans Genotype: anc-1(e1802) I. Description: Nuclei not anchored in certain epithelial cells. Mutagen: EMS Outcrossed: x Made by: Received: 12/01/82 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3339 Species: Caenorhabditis elegans Genotype: anc-1(e1753) I. Description: Nuclei not anchored in certain epithelial cells. Mutagen: EMS Outcrossed: x Made by: Received: 12/01/82 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3353 Species: Caenorhabditis elegans Genotype: mab-9(e1245) II; him-5(e1490) dpy-21(e428) V. Description: Hermaphrodites are Dpy and segregates males. Males are non-Dpy and have severe tail abnormalities; frequently lethal to adult males. Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 02/01/82 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3383 Species: Caenorhabditis elegans Genotype: cat-6(e1861) V. Description: Sensory neurons CEP, ADE, PDE take up FITC; tubular body of CEP cilia disrupted; slightly defective dauer formation. Mutagen: EMS Outcrossed: x1 Made by: Ed Hedgecock Received: 06/01/85 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: CB3387 Species: Caenorhabditis elegans Genotype: ced-1(e1735) I; ced-2(e1752) IV. Description: Mutagen: Outcrossed: x Made by: Ed Hedgecock Received: 07/08/04 MIT, Cambridge MA, USA ----------------------------------------------------------------------------- Strain: CB3388 Species: Caenorhabditis elegans Genotype: ncl-1(e1865) III. Description: Abnormal large nucleoli in all cells. Mutagen: Outcrossed: x Made by: Received: 11/01/85 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: CB3397 Species: Caenorhabditis elegans Genotype: eDf20 III; eDp6 (III;f) Description: Wild type strain segregating more wild type and embryonic lethals, in approximately 1:1 ratio. Mutagen: X Rays Outcrossed: x>3 Made by: J. Hodgkin Received: 12/29/93 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3432 Species: Caenorhabditis elegans Genotype: anc-1(e1802) dpy-5(e61) I. Description: Dpy. Nuclei not anchored in certain epithelial cells. Mutagen: Outcrossed: x Made by: Received: 12/03/82 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3439 Species: Caenorhabditis elegans Genotype: lin-16(e1743) III; mnDp37 (III;f). Description: Animals which have lost the Dup are thin, sterile, and uncoordinated. Maintain by picking WT (Egl). Mutagen: Outcrossed: x Made by: M. Chalfie Received: 04/04/88 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3440 Species: Caenorhabditis elegans Genotype: anc-1(e1873) I. Description: e1873 allele is the only anc-1 allele in which the molecular lesion has been identified (Starr and Han, 2002) and is considered by the authors to be the reference allele. e1873 does not revert, unlike most other alleles of anc-1. Reference: Hedgecock and Thomson. Cell. 1982 Aug;30(1):321-30. Starr and Han. Science. 2002 Oct 11;298(5592):406-9. Mutagen: EMS Outcrossed: x Made by: Ed Hedgecock Received: 09/27/16 University of California, Davis, CA ----------------------------------------------------------------------------- Strain: CB3474 Species: Caenorhabditis elegans Genotype: ben-1(e1880) III. Description: Benomyl Resistant. Dominant at 25C--Recessive at 15C. Mutagen: EMS Outcrossed: x Made by: Received: 06/01/85 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: CB3475 Species: Caenorhabditis elegans Genotype: mcm-4(e1466)/szT1 [lon-2(e678)] I; +/szT1 X. Description: Heterozygotes are WT and segregate WT, Lon males and thin sterile Unc animals. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Horvitz/Chalfie?? Received: 04/01/84 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: CB3497 Species: Caenorhabditis elegans Genotype: dpy-25(e817) II. Description: Dpy. Severe. Semidominant. Inviable at 15C. Mutagen: EMS Outcrossed: x0 Made by: Hodgkin J Received: 02/01/82 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB351 Species: Caenorhabditis elegans Genotype: unc-65(e351) V. Description: Slightly Unc-cannot back. Recessive. M-MATING++ 1-10%WT. CGC received new stock 6/01. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 09/01/77 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3514 Species: Caenorhabditis elegans Genotype: lin-23(e1883)/dpy-10(e128) II. Description: Heterozygotes are WT and segregate WT, Dpy, and Long Thin Steriles (adults lay no or few eggs). lin-23 animals have excess cell divisions in larval blast cell lineages. Mutagen: EMS Outcrossed: x Made by: Ed Hedgecock Received: 11/10/00 University of Georgia, Athens, GA ----------------------------------------------------------------------------- Strain: CB3518 Species: Caenorhabditis elegans Genotype: mab-10(e1248) II; him-5(e1490) V. Description: Hermaphrodtes segregate impotent males. Males show very slight swelling of bursa. Mutagen: EMS Outcrossed: x Made by: Hodgkin J Received: 02/01/82 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3531 Species: Caenorhabditis elegans Genotype: mab-5(e1239) III; him-5(e1490) V. Description: Males abnormal. Mutagen: Outcrossed: x Made by: Received: 04/20/93 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CB3533 Species: Caenorhabditis elegans Genotype: +/szT1 [lon-2(e678)] I; twk-18(e1913)/szT1 X. Description: Heterozygotes are Unc and segregate Unc, dead eggs, and Lon males. e1913 is a dominant Unc and recessive lethal. Maintain by picking Unc. e1913 previously called unc-110. Mutagen: EMS Outcrossed: x2 Made by: Jonathan Hodgkin Received: 04/21/93 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3549 Species: Caenorhabditis elegans Genotype: ncl-2(e1896)/dpy-13(e184) IV. Description: Heterozygotes are weakly Dpy and segregate more weak Dpys, dpy-13 homozygotes and ncl-2 homozygotes. ncl-2 homozygotes are viable, sterile animals with abnormal nucleoli (abnormal refractile appearance by Nomarski). Imperfectly balanced: May Break Down!! Mutagen: EMS Outcrossed: x1 Made by: Ed Hedgecock Received: 12/21/93 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB355 Species: Caenorhabditis elegans Genotype: unc-65(e355) V. Description: Stronger phenotype than e351. Mutagen: EMS Outcrossed: x Made by: Sydney Brenner Received: 06/27/01 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3574 Species: Caenorhabditis elegans Genotype: lin-30(e1908) III. Description: Variable failures in cytokinesis. Mostly viable and fertile. Mutagen: EMS Outcrossed: x1 Made by: Ed Hedgecock Received: 07/13/94 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3610 Species: Caenorhabditis elegans Genotype: mig-6(e1931)/dpy-11(e224) V. Description: Heterozygotes are WT and segregate WT, Dpys, and steriles. mig-6(e1931) hermaphrodites are sterile, with failure of DTC migrations. Gonad sterile, disorganized, otherwise hermaphrodites appear normal. Strain is imperfectly balanced - check for correct segregation of Dpy and Mig from WT hermaphrodites. Mutagen: EMS Outcrossed: x Made by: E.M. Hedgecock Received: 09/02/97 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3616 Species: Caenorhabditis elegans Genotype: tra-3(e1107) dpy-4(e1166)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Dpy, Vul, and dead eggs. The Dpys throw Dpy males. Maintain by picking WT. Mutagen: Outcrossed: x Made by: Kimble J Received: 07/01/83 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: CB362 Species: Caenorhabditis elegans Genotype: unc-44(e362) IV. Description: Paralyzed Unc. Small. Recessive. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3631 Species: Caenorhabditis elegans Genotype: tra-1(e1929) III; eDp6 (III;f). Description: WT hermaphrodites which segregate WT hermaphrodites and tra-1 homozygous XX; weakly masculinized class A7, never fertile as males. Mutagen: EMS Outcrossed: x2 Made by: Hedgecock/Hodgkin Received: 11/14/01 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB364 Species: Caenorhabditis elegans Genotype: dpy-18(e364) III. Description: Dpy. Mapping marker standard. Recessive. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3687 Species: Caenorhabditis elegans Genotype: che-14(e1960) I. Description: Some FITC uptake by amphids but not by phasmids. Hypodermal defect. Mutagen: EMS Outcrossed: x Made by: Received: 06/01/85 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: CB369 Species: Caenorhabditis elegans Genotype: unc-51(e369) V. Description: Paralyzed Unc. Recessive. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3695 Species: Caenorhabditis elegans Genotype: fem-1(e1965)/unc-5(e53) mor-2(e1125) IV. Description: WT heterozygote, fem-1(e1965) maternal. Segregates WT heterozygotes, WT fem-1 homozygotes that give only fertile female progeny, and UncMor. (Probably has lost mor-2 [Edgley, 4/92]. When confirmed, should be given a new strain name.) Mutagen: EMS Outcrossed: x1 Made by: T. Doniach/J Hodgkin Received: 03/01/85 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3737 Species: Caenorhabditis elegans Genotype: sup-29(e1986) tra-3(e1903) IV. Description: WT phenotype. tra-3 amber mutant carrying homozygous linked amber suppressor. Mutagen: EMS Outcrossed: x0 Made by: J. Hodgkin Received: 04/04/88 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3740 Species: Caenorhabditis elegans Genotype: eDf24 I; eDp20 (I;II); mnT12 (IV;X). Description: Phenotypically WT. See also CGC 801. eDf24 = let(e2000). Mutagen: Outcrossed: x Made by: Received: 09/01/85 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB376 Species: Caenorhabditis elegans Genotype: unc-13(e376) I. Description: Slightly Unc. Suppressed by sup-5 and sup-7. Null allele? Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 08/01/82 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: CB3769 Species: Caenorhabditis elegans Genotype: tra-1(e1575)/+ III; tra-3(e1767) IV. Description: Stable male/female strain propagated by crossing tra-3(e1767)IV females x tra-3(e1767)IV males. tra-1(e1575) is semi-dominant and transforms both XX and XO into fertile females. Strain contains 4 genotypes: 1) e1575/+; e1767 XX which are fertile females. 2) e1575/+; e1767 XO which are fertile females. 3) e1767 XX which are infertile pseudomales. 4) e1767 XO which are fertile males. Mutagen: Outcrossed: x Made by: J. Hodgkin Received: 03/01/85 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3775 Species: Caenorhabditis elegans Genotype: dpy-20(e2017) IV. Description: Severe roundheaded Dpy. Almost inviable at 15C. Mutagen: EMS Outcrossed: x2 Made by: J. Hodgkin Received: 03/01/86 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3778 Species: Caenorhabditis elegans Genotype: tra-2(e2020) II. Description: Obligate XX female, XO male strain. Maintain by mating. e2020 is a dominant mutation transforming XX into fertile females. XO animals are partly feminized by starvation or elevated temperatures, therefore the strain cannot be grown at 25C. Mutagen: EMS Outcrossed: x1 Made by: T. Doniach Received: 08/12/93 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3779 Species: C. elegans Genotype: tra-2(e2021) II. Description: tra-2 gain-of-function. Male-female strain. Maintain by mating. References: EMBO J. 2001 Mar 15; 20(6): 1363–1372. Proc Natl Acad Sci U S A. 2004 Aug 24; 101(34): 12549–12554. Mutagen: unknown Outcrossed: x? Made by: T. Doniach Received: 07/03/18 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: CB3816 Species: Caenorhabditis elegans Genotype: tra-3(e1107) IV; unc-58(e665) sup-21(e1957) dpy-6(e14)/+ X. Description: Heterozygotes are Unc (shaker; don't move well) and segregate more Shaker Unc, WT and DpyUnc (short and fairly paralyzed). The WT give only males. e1957 previously called sup-21. Mutagen: EMS Outcrossed: x Made by: Hodgkin Received: 05/01/85 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB382 Species: Caenorhabditis elegans Genotype: unc-49(e382) III. Description: Shrinker-contracts both dorsally and ventrally when prodded. Slow, poor backing. Slightly small. M-MATING++ 1-10%WT Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3823 Species: Caenorhabditis elegans Genotype: eDf18/unc-24(e138) dpy-20(e1282) IV. Description: Heterozygotes are WT and segregate WT, DpyUnc and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x1 Made by: J. Hodgkin Received: 08/01/85 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3824 Species: Caenorhabditis elegans Genotype: eDf19/unc-24(e138) dpy-20(e1282) IV. Description: Heterozygotes are WT (somewhat Unc and Egl) and segregate WT, DpyUnc and dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x1 Made by: J. Hodgkin Received: 08/01/85 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB384 Species: Caenorhabditis elegans Genotype: unc-11(ic9) unc-63(e384) I. Description: Unc. Recessive. Levamisole resistant. M-MATING++ 1-10%WT. Also contains an unc-11 mutation->Aixa Alfonso 10/94. See WBPaper00002195. Use ZZ37 for unc-63 reference strain. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 06/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB3843 Species: Caenorhabditis elegans Genotype: fem-3(e1996)/unc-24(e138) dpy-20(e1282) IV. Description: Heterozygotes are WT and segregate WT, DpyUnc and fertile females. Mutagen: EMS Outcrossed: x Made by: J. Hodgkin Received: 03/01/86 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3844 Species: Caenorhabditis elegans Genotype: fem-3(e2006) IV. Description: Temperature sensitive Fem. Hermaphrodites at 15C, female at 25C. Maintain at 15C. Mutagen: EMS Outcrossed: x Made by: J. Hodgkin Received: 12/16/94 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3855 Species: Caenorhabditis elegans Genotype: plg-1(e2001) III; him-5(e1490) V. Description: Hermaphrodites and males superficially WT. Males lay down a gelatinous plug over the vulva of mated hermaphrodites. Plug formation controlled only by male genotype. Both plg-1 and plg-1/+ males make plugs. Mutagen: Outcrossed: x Made by: C. Kenyon Received: 02/01/87 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3874 Species: Caenorhabditis elegans Genotype: dpy-20(e2017) IV; sup-28(e2058) X. Description: Partly or completely suppressed Dpy. Mutagen: EMS Outcrossed: x Made by: Hodgkin Received: 04/04/88 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3875 Species: Caenorhabditis elegans Genotype: sup-23(e2059) dpy-20(e2017) IV. Description: Weak suppression of dpy-20 by sup-23. Medium Dpy. Mutagen: EMS Outcrossed: x Made by: J. Hodgkin Received: 03/01/86 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3908 Species: Caenorhabditis elegans Genotype: sup-22(e2057) dpy-20(e2017) IV. Description: Weak suppression of dpy-20 by sup-22. Dpy phenotype, but less Dpy than dpy-20 alone. Mutagen: EMS Outcrossed: x Made by: J. Hodgkin Received: 03/01/86 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3909 Species: Caenorhabditis elegans Genotype: dpy-20(e2017) IV; sup-21(e1957) X. Description: Suppressed Dpy. e1957 previously called sup-21. Mutagen: Outcrossed: x Made by: Jonathan Hodgkin Received: 09/03/96 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3911 Species: C. elegans Genotype: dpy-27(rh18) III; 4A;3X. Description: Non-Dpy 4A;3X hermaphrodites segregating 4A;3X hermaphrodites, 4A;2X males and dead or very dumpy 4A;4X hermaphrodites. Reference: Strain 19 in Hodgkin (2002) PMID: 12399387 Mutagen: EMS Outcrossed: x2 Made by: J. Hodgkin Received: 08/24/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3912 Species: Caenorhabditis elegans Genotype: eT2 (X;I)/unc-2(e55) X. Description: Heterozygotes are WT and segregate WT, Unc and dead embryos. eT2 homozygotes are embryonic lethals. Mutagen: Outcrossed: x Made by: Cynthia Kenyon Received: 02/27/95 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: CB3924 Species: Caenorhabditis elegans Genotype: pal-1(e2091) III; him-5(e1490) V. Description: Males are missing most of the rays and additional alae extend into the tail. Mutagen: EMS Outcrossed: x2 Made by: Cynthia Kenyon Received: 04/20/93 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CB3967 Species: Caenorhabditis elegans Genotype: tra-2(e2046) II. Description: Obligate XX female, XO male strain. Maintain by mating. Viable 15-25C. e2046 is a dominant mutation transforming XX into fertile female; minimal effect on XO. Mutagen: X Rays Outcrossed: x4 Made by: J. Hodgkin Received: 08/12/93 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3970 Species: Caenorhabditis elegans Genotype: unc-4(e120) bli-1(e769) II. Description: Uncoordinated and Blistered. Mutagen: Outcrossed: x Made by: Michael Shen Received: 02/14/94 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB398 Species: Caenorhabditis elegans Genotype: mec-8(e398) I. Description: Slightly Dpy. Sluggish. Recessive. Mechanosensory abnormal. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB3988 Species: Caenorhabditis elegans Genotype: tra-3(e1107) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Hets are Unc and segregate Unc, WT hermaphrodites and dead eggs. The WT hermaphrodites segregate only abnormal sterile males or intersexes. Maintain by picking Unc. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 09/21/87 Western Washington University, Bellingham, WA ----------------------------------------------------------------------------- Strain: CB3989 Species: C. elegans Genotype: tra-1(q88) III; eDp6 (III;f). Description: Wildtype hermaphrodites segregating wild-type hermaphrodites and tra-1(q88) XX males, which are fertile and make yolk. Complex rearrangement of tra-1. Reference: Zarkower et al. (1994) PMID: 7520378 Mutagen: EMS Outcrossed: x3 Made by: J. Hodgkin Received: 08/24/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB3991 Species: Caenorhabditis elegans Genotype: sma-8(e2111) V. Description: Short, blunt head. Dominant. Mutagen: EMS Outcrossed: x3 Made by: J. Hodgkin Received: 10/01/85 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4000 Species: Caenorhabditis elegans Genotype: sma-1(e30) V. (high Tc1 copy number) Description: High Tc1 copy number, arose spontaneously in CB30. Him. Small, roundheaded. Mutagen: Outcrossed: x Made by: Brenner S Received: 10/01/85 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4015 Species: C. elegans Genotype: fem-3(e1999) IV. Description: Weakest known fem-3 mutation. Viable non-temperature-sensitive self-fertile XX hermaphrodites. e1999 XO males are slightly feminized, sometimes male-fertile. Reference: Hodgkin (1986) PMID: 3770465. Mutagen: EMS Outcrossed: x3 Made by: J. Hodgkin Received: 08/24/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4017 Species: Caenorhabditis elegans Genotype: fem-1(hc17) him-8(e1489) IV; unc-1(e1598) X. Description: Severely Uncoordinated; Him; feminized at temperatures above 20C. Grow at 15C. Used for generating patroclinous WT males, by exploiting non-disjunction caused by him-8, and dominant sex-linked unc-1. Mutagen: Outcrossed: x Made by: Jonathan Hodgkin Received: 12/07/98 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB402 Species: Caenorhabditis elegans Genotype: unc-55(e402) I. Description: Unc. Recessive. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4027 Species: Caenorhabditis elegans Genotype: tra-3(e1107) eIs2137 IV. Description: eIs2137 [sup-7(+) + Dm-hsp::lacZ] IV. lacZ expression induced by heat shock. Limited pattern (the pSHZ1 vector was originally designed for use in Drosophila, not C. elegans, and is not the best vector for expression of lacZ in the worm). This strain has predominantly historical value (as the first C. elegans strain shown to express a foreign gene). eIs2137 transgene is integrated and contains two plasmids: pAST (sup-7 amber suppressor), and pSHZ1 (lacZ driven by Drosophila heat shock promoter (S. Munro)). Mutagen: Outcrossed: x Made by: A. Fire Received: 04/09/02 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: CB403 Species: Caenorhabditis elegans Genotype: unc-29(e403) I. Description: Unc. Recessive. M-MATING++ 1-10%WT. W176Stop, a "C" to "T" mutation at 3305 on the T08G11 cosmid. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4035 Species: Caenorhabditis elegans Genotype: fem-2(e2105)/unc-45(r450) dpy-1(e1) III. Description: Heterozygotes are WT and segregate WT and DpyUnc. 1/3 of the WT are fem-2 homozygotes. Homozygous fem-2 animals are hermaphrodites if mother was heterozygous for fem-2, and are fertile females if mother was homozygous for fem-2. Mutagen: Outcrossed: x Made by: J. Hodgkin Received: 03/01/86 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4037 Species: Caenorhabditis elegans Genotype: glp-1(e2141) III. Description: Temperature sensitive. Sterile at 25C. Maintain at 15C. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. [2/98: Craig Mello noticed a different embryonic phenotype in this strain as compared to the e2141 stock that Jim Priess obtained from England-the ABp fate appears WT.] [NOTE (11/16/10 - J. Hubbard): This strain is NOT synonymous with glp-1(e2144) as previously reported in Kodoyianni V, Maine EM, Kimble J. (1992) [Molecular basis of loss-of-function mutations in the glp-1 gene of Caenorhabditis elegans. Mol Biol Cell. 3,1199-213. PMID: 1457827]. As reported in Worm Breeders Gazette December 2010; 18(3), e2144 carries the mutation c2785t in exon 8, leading to the amino acid change L929F, whereas e2141 carries the mutations c2920t and a3610g in exon 8, leading to the amino acid changes R974C and T1204A.] Mutagen: EMS Outcrossed: x Made by: Schnabels Received: 04/03/96 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: CB404 Species: Caenorhabditis elegans Genotype: unc-53(e404) II. Description: Unc-cannot back. Males abnormal. Bursae abnormal. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 09/01/77 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4043 Species: Caenorhabditis elegans Genotype: smg-2(e2008) I; him-5(e1490) V. Description: e2008 recessively suppresses the phenotypes of mutations in unc-54(r293), lin-29(n546), tra-2 and dpy-5. Abnormal male tail. smg-2 homozygotes have slightly abnormal movement. Mutagen: Outcrossed: x Made by: J. Hodgkin Received: 01/28/88 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4050 Species: C. elegans Genotype: tra-2(e2046e2115) unc-4(e120) II; sup-7(st5) X. Description: Phenotypically wildtype XX hermaphrodite at 20C; poor viability and fertility at 25C. Rare XO animals are hermaphrodite due to suppression of amber mutation e2115 and consequent transformation of XO due to tra-2(e2046) gain-of-function. Reference: Hodgkin & Albertson (1995) PMID: 8647390. Mutagen: EMS Outcrossed: x2 Made by: J. Hodgkin Received: 08/27/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4053 Species: C. elegans Genotype: egl-26(e1952) II; tra-3(e1767) IV. Description: Self-fertile intersexual XX animals. Tra-3 masculinized phenotype suppressed by egl-26 mutation. Reference: Hodgkin (1986) PMID: 3770465. Mutagen: EMS Outcrossed: x2 Made by: J. Hodgkin Received: 08/31/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB406 Species: Caenorhabditis elegans Genotype: unc-57(e406) I. Description: Unc. Recessive. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB407 Species: Caenorhabditis elegans Genotype: unc-49(e407) III. Description: Uncoordinated, shrinker. Mutagen: EMS Outcrossed: x1 Made by: S. Brenner Received: 11/01/99 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4077 Species: Caenorhabditis elegans Genotype: eDf21/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: WT heterozygotes segregate WT, DpyUnc and unhatched eggs. Mutagen: Outcrossed: x Made by: M. Shen Received: 01/31/89 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB408 Species: Caenorhabditis elegans Genotype: unc-43(e408) IV. Description: Marginally Unc-no sin wave. Lethargic. Recessive. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB4088 Species: Caenorhabditis elegans Genotype: him-5(e1490) V. Description: From CB1490, which is a double mutant of him-5 with ali-1. CB4088 has been outcrossed to remove ali-1. Mutagen: Outcrossed: x Made by: J. Hodgkin Received: 08/29/08 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4104 Species: Caenorhabditis elegans Genotype: mab-12(e2166) IV; him-5(e1490) V. Description: Males have abnormal swollen bursa. Hermaphrodites have abnormal swollen vulva. Mutagen: EMS Outcrossed: x1 Made by: S. Emmons Received: 01/28/88 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4108 Species: Caenorhabditis elegans Genotype: fog-2(q71) V. Description: Male/female strain. Maintain by mating. XX animals are female. XO animals are WT males. Mutagen: Outcrossed: x Made by: Received: 07/13/94 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4111 Species: Caenorhabditis elegans Genotype: mab-24(e2169) I; him-5(e1490) V. Description: Anterior displacement of rays and fan. Ray identities are not altered. Slightly Unc (coiler). Mutagen: EMS Outcrossed: x>5 Made by: Scott Emmons Received: 04/14/93 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: CB4118 Species: Caenorhabditis elegans Genotype: unc-32(e189) ooc-4(e2078)/eDf2 III. Description: Heterozygotes are WT and segregate WT, Uncs which are sterile and dead eggs (eDf2 homozygotes). Strain breaks down very rarely. Mutagen: EMS Outcrossed: x2 Made by: T. Doniach Received: 11/15/93 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4121 Species: Caenorhabditis elegans Genotype: sqt-3(e2117) V. Description: Viable Dpy at 15C. Extreme Dpy or L1 lethal at 25C. Mutagen: EMS Outcrossed: x1? Made by: Schnabels Received: 11/01/99 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4123 Species: Caenorhabditis elegans Genotype: lon-3(e2175) V. Description: Long, especially obvious in adults. Males mate well. Mutagen: Spontaneous in TR679 Outcrossed: x2 Made by: R. Hoskins Received: 07/10/89 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4147 Species: Caenorhabditis elegans Genotype: fog-1(e2121) unc-11(e47) I; sDp2 (I;f). Description: Animals with the Dup are WT. Animals which have lost the Dup are Unc and female. Maintain by picking WT. Mutagen: EMS Outcrossed: x>5 Made by: Tabitha Doniach Received: 09/01/86 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB419 Species: Caenorhabditis elegans Genotype: unc-42(e419) V. Description: Unc. Recessive. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4219 Species: Caenorhabditis elegans Genotype: pal-2(e2260) IV; him-5(e1490) V. Description: WT hermaphrodites. Mails have ectopic lateral alae in tail region. Segregates males. Mutagen: EMS Outcrossed: x1 Made by: M. Shen Received: 02/01/93 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4227 Species: Caenorhabditis elegans Genotype: sup-34(e2227) I; unc-24(e138) dpy-20(e2017) IV. Description: sup-34 is dominant amber suppressor: weak suppression of Unc, medium suppression of Dpy. Mutagen: EMS Outcrossed: x1 Made by: Jonathan Hodgkin Received: 02/01/93 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4270 Species: C. elegans Genotype: tra-1(e2200) Description: Hermaphrodite at 15C, sterile and gonad-defective at 25C; feminized by smg (NMD) mutations. Mutagen: EMS Outcrossed: x2 Made by: J. Hodgkin Received: 10/04/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB428 Species: Caenorhabditis elegans Genotype: dpy-21(e428) V. Description: Dpy hermaphrodites. Males (XO) non-Dpy. M-MATING++++ >30%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB4281 Species: Caenorhabditis elegans Genotype: +/eT1 III; eDf43 dpy-11(e224)/eT1 V. Description: eDf43 pka lin-49(e2173). Heterozygotes are WT and segregate WT, Unc-36, and LinDpys. The lin-40 dpy-11 homozygotes are Dpy, Sterile and abnormal. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: S. Emmons/J. Hodgkin Received: 07/13/94 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4289 Species: C. elegans Genotype: tra-1(e1575e1816e1835) III; eDp6 (III;f). Description: Wildtype hermaphrodites segregating wildtype hermaphrodites and tra-1 XX males, which mate with greater efficiency than most tra-1 XX mutants. Reference: Hodgkin (1987) PMID:3428597. Mutagen: EMS Outcrossed: x3 Made by: J. Hodgkin Received: 08/24/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4330 Species: Caenorhabditis elegans Genotype: unc-4(e2309) II. Description: Reference: Mutagen: Outcrossed: x Made by: Received: 11/20/12 Vanderbilt University, Nashville, TN ----------------------------------------------------------------------------- Strain: CB4354 Species: Caenorhabditis elegans Genotype: smg-3(ma117) IV; him-5(e1490) V. Description: Hermaphrodites have an abnormal protrusive vulva. Segregate males with abnormally swollen bursa. Suppressor of certain alleles of certain genes. Mutagen: Outcrossed: x Made by: J. Hodgkin Received: 01/28/88 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4355 Species: Caenorhabditis elegans Genotype: him-8(e1489) IV; smg-4(ma116) V. Description: Hermaphrodites have an abnormal protrusive vulva. Segregate males with abnormally swollen bursa. ma116 is a weak recessive suppressor of certain alleles of certain genes. Mutagen: Outcrossed: x Made by: Received: 01/28/88 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4370 Species: Caenorhabditis elegans Genotype: unc-117(e2330) X. Description: Spontaneous mutation in TR679. Adults slightly Unc, slow and loopy. Larvae more affected, slow moving. Mutagen: Spontaneous-TR679 Outcrossed: x Made by: D. Thierry-Mieg Received: 04/21/93 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4371 Species: Caenorhabditis elegans Genotype: unc-118(e2331) X. Description: Spontaneous mutation in parental strain TR679. Adults backward coiler, slow forward, slight kinker. Larvae more affected, strong kinkers. Variable penetrance. Mutagen: Spontaneous-TR679 Outcrossed: x Made by: D. Thierry-Mieg Received: 04/21/93 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4374 Species: Caenorhabditis elegans Genotype: spg- (e2335) III; unc-31(e928) IV. Description: Spongy gut, intestine has foamy look, especially in L4; Temperature sensitive-almost normal at 15C; leaky Eat at 20C; Penetrant and sick Eat at 25C. Unc. Mutagen: EMS Outcrossed: x Made by: Received: 07/17/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: CB4377 Species: C. elegans Genotype: lin-22(n372) pal-2(e2260) IV; him-5(e1490) V; lin-32(e1926) X. Description: Hermaphrodites segregating males with abnormal ray lineages. Reference: Hodgkin et al. (1989) Genome, 31, 625-637. doi:10.1139/g89-116. Mutagen: Outcrossed: x1 Made by: M. M. Shen Received: 08/24/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4389 Species: C. elegans Genotype: tra-2(e1209) II; smg-3(ma117) IV. Description: Poorly growing, low self-fertility masculinized XX hermaphrodites. Weak allele of tra-2, partly suppressed to self-fertility by smg (NMD) mutation; permits efficient selection of new feminizing mutations. References: Spence et al. (1990) PMID: 2317869. Zarkower et al. (1994) PMID: 7520378. Mutagen: Outcrossed: x2 Made by: J. Hodgkin Received: 08/24/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4391 Species: Caenorhabditis elegans Genotype: unc-31(e928) unc-26(e2340) IV. Description: Unc. Eat. Mutagen: Outcrossed: x Made by: Received: 02/22/94 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: CB4394 Species: Caenorhabditis elegans Genotype: eat-1(e2343) unc-31(e928) IV. Description: Thin, starved, healthy adults. Retain few eggs. Slow irregular pumping at 16, 20, 25C. Unc. Mutagen: EMS Outcrossed: x Made by: Leon Avery Received: 09/20/93 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: CB4419 Species: Caenorhabditis elegans Genotype: tra-3(e2333) IV. Description: ZZ21 lev-1(x21) tra-3(e2333) isolated by Jim Lewis as EMS-induced levamisole resistant derivative of N2. Found to carry a cryptic tra-3 allele by Jonathan Hodgkin. XX animals are viable hermaphrodites which produce 500 rather than 330 self-progeny; occasionally Egl; no other signs of masculinization. Mutagen: from ZZ21 Outcrossed: x3 Made by: J. Hodgkin Received: 11/01/99 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4425 Species: Caenorhabditis elegans Genotype: tra-3(e1107) sup-24(st354) IV. Description: Wild type hermaphrodite phenotype, since tra-3(e1107) is fully suppressed. Mutagen: Outcrossed: x Made by: Jonathan Hodgkin Received: 05/31/95 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4435 Species: Caenorhabditis elegans Genotype: dpy-20(e2017) sup-24(st354) IV. Description: Extreme Dpy suppressed to weak Dpy or non-Dpy phenotype by amber suppressor. Received new stock from J. Hodgkin 5/31/95. Mutagen: EMS Outcrossed: x Made by: Jonathan Hodgkin Received: 02/01/93 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB444 Species: Caenorhabditis elegans Genotype: unc-52(e444) II. Description: Unc. Larvae move. Paralyzed adult. Dystrophic body muscle. M-MATING-NO SUCCESS. Recessive. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4457 Species: C. elegans Genotype: fem-1(e2342) / unc-5(e53) mor-2(e1125) IV. Description: Wild-type hermaphrodites segregating WT, Fem, Unc Mor. Maintain by picking wild-type hermaphrodites. Deletion allele of fem-1, with unusual maternal effect. Reference: Spence AM, et al. Cell. 1990 Mar 23;60(6):981-90. Johnson CL & Spence AM.. Science. 2011 Sep 2;333(6047):1311-4. Mutagen: 32P mutagenesis of N2 Outcrossed: x6 Made by: Andrew M. Spence Received: 08/27/18 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4461 Species: Caenorhabditis elegans Genotype: twk-18(e1913e2383) X. Description: Wild type. previously called unc-110. Mutagen: EMS Outcrossed: x7 Made by: D. Thierry-Mieg Received: 04/21/93 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB448 Species: Caenorhabditis elegans Genotype: unc-24(e448) IV. Description: Unc. Recessive. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB450 Species: Caenorhabditis elegans Genotype: unc-13(e450) che-3(ky1018) I. Description: Paralyzed Unc. This strain carries a tightly linked mutation, che-3(ky1018), that changes Q1107 of che-3 to a stop codon. Reference: Larsch, et al. (2015) Cell Reports. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4504 Species: Caenorhabditis elegans Genotype: gon-1(e1254)/eDf18 IV. Description: Heterozygotes mostly fertile at or below 20C; all sterile at 25C. Progeny are fertile heterozygotes with variable Gon abnormality, e1254 homozygotes (strong Gon, "white patch" phenotype) and eDf18 homozygotes (embryonic lethal). See also WBPaper00003841. Mutagen: EMS Outcrossed: x3 Made by: J. Hodgkin Received: 11/15/93 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4507 Species: Caenorhabditis elegans Genotype: C. briggsae wild isolate. Description: Wild isolate from Palm Canyon, CA. Segregated to remove gro-1(e2400) [CB4512]. Caenorhabditis elegans wild isolate CB gro-1(+) subclone of PaC1 (Tc1 pattern IV). Mutagen: Outcrossed: x Made by: Received: 01/31/91 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4512 Species: Caenorhabditis elegans Genotype: gro-1(e2400) III. Description: Slow growing. Homozygous gro-1(e2400) derived from PaC-1 (CB4507). Caenorhabditis elegans wild isolate CB subclone of PaC1 (Tc1 pattern IV?)]. Mutagen: Outcrossed: x Made by: Hodgkin J Received: 01/31/91 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4549 Species: Caenorhabditis elegans Genotype: dpy-11(e224) smg-4(ma116) V. Description: Dpy hermaphrodites. Protruding vulva in adult; viable at all temperatures. Mutagen: Outcrossed: x Made by: J. Hodgkin Received: 02/05/98 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4550 Species: Caenorhabditis elegans Genotype: dpy-1(e1) smg-6(r896) III. Description: Dpy hermaphrodites. Protruding vulva in adult; viable at all temperatures. Mutagen: Outcrossed: x Made by: J. Hodgkin Received: 02/05/98 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4554 Species: C. elegans Genotype: smg-2(e2008) smg-1(e1228) I; him-5(e1490) V. Description: Smg (protruding vulva) hermaphrodites segregating Smg hermaphrodites and Smg (Male abnormal) males. Reference: Hodgkin et al. (1989) PMID: 2583479. Mutagen: EMS Outcrossed: x2 Made by: J. Hodgkin Received: 08/24/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4555 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Wild isolate from Pasadena, Ca. [Flower bed on CIT campus in summer of 1971 (or 1973??). Wild type phenotype. High copy Tc1. Reference WBG 10(2) 140-141. Caenorhabditis elegans wild isolate. CB subclone of PA1 (Tc1 pattern HCG?). Mutagen: Outcrossed: x Made by: Hedgecock E Received: 01/31/91 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4559 Species: C. elegans Genotype: smg-2(e2008) I; tra-1(e1732) III. Description: Grows poorly, best at 20C. Self-fertile intersexual XX hermaphrodite due to partial suppression of weak tra-1 allele by smg-2(NMD). References: Zarkower et al. (1994) PMID: 7520378. Hodgkin (1987) PMID:3428597. Mutagen: Outcrossed: x2 Made by: J. Hodgkin Received: 08/24/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4567 Species: Caenorhabditis elegans Genotype: unc-53(e2432) II. Description: Unc-cannot back. Egl. Males abnormal. Multiple defects in neuronal outgrowth and branching, also defects in excretory canal extension and in sex muscles migration. Mutagen: EMS Outcrossed: x Made by: T. Bogaert Received: 10/31/02 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: CB4570 Species: Caenorhabditis elegans Genotype: lev-1(x577) gro-2(e2442) IV. Description: Levamisole resistant. Slow growing. Mutagen: EMS Outcrossed: x0 Made by: J. Lewis/T. Barnes Received: 02/01/93 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB458 Species: Caenorhabditis elegans Genotype: dpy-13(e458) IV. Description: Mutagen: EMS Outcrossed: x1 Made by: S. Brenner Received: 07/01/86 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4605 Species: Caenorhabditis elegans Genotype: mab-9(e2410) II; him-5(e1490) V. Description: Mutagen: Outcrossed: x Made by: Received: 08/27/96 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4613 Species: C. elegans Genotype: unc-32(e189) tra-1(e1575e1816e1831) III; eDp6 (III;f). Description: Unc-32 hermaphrodites segregating Unc hermaphrodites and Unc Tra homozygous XX pseudomales with variable male tails, hermaphroditic gonads and adult yolk production. Class B2 allele (male tail, sterile hermaphrodite gonad) derived by mutation of tra-(gf) allele e1575. Reference: Hodgkin (1987) PMID:3428597. Mutagen: EMS Outcrossed: x3 Made by: J. Hodgkin Received: 08/24/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4628 Species: C. elegans Genotype: tra-2(e1095) II; fem-1(e1927) IV; xol-1(y9) X. [XX females and tra-2; fem-1/+; xol-1 XX males] Description: Obligate male/female strain; maintain by crossing. Anatomically normal XX females and XX males. Male/female strain with sex determined by fem-1(+). Reference: Strain 11 in Hodgkin (2002) PMID: 12399387. Mutagen: Outcrossed: x2 Made by: J. Hodgkin Received: 08/24/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4681 Species: Caenorhabditis elegans Genotype: nDf17/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are Dpy and segregate Dpy, Sterile Dpys and dead eggs. [CGC received new stock in 1/1999 from Leon Avery.] Mutagen: Gamma Rays Outcrossed: x Made by: Joel Rothman Received: 01/04/99 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: CB4689 Species: Caenorhabditis elegans Genotype: dpy-28(y1) III; him-8(e1489) IV. Description: y1 is a temperature sensitive mutation affecting only XX animals. him-8(e1489) causes X non-disjunction, resulting in many males. Grow at 15C. XX and XXX animals not viable above 15C. After shifting to 25C, surviving population is almost all XO males. Mutagen: Outcrossed: x Made by: Jonathan Hodgkin Received: 12/07/98 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB47 Species: Caenorhabditis elegans Genotype: unc-11(e47) I. Description: Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB4706 Species: C. elegans Genotype: tra-2(e1095) II; fem-1(e1927) IV; xol-1(y9) X. [XX females and tra-2; fem-1; xol-1; eEx14[fem-1(+)] XX males] Description: Obligate male/female strain; maintain by crossing. Anatomically normal XX females and XX males. Male/female strain with sex determined by an extrachromosomal transgene array carrying fem-1(+). Reference: Strain 16 in Hodgkin (2002) PMID: 12399387. Mutagen: Outcrossed: x3 Made by: J. Hodgkin Received: 08/24/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4711 Species: Caenorhabditis elegans Genotype: mab-5(e1239) egl-5(n945) III. Description: n945: HSN-. Egl. Coiler. Mutagen: EMS Outcrossed: x Made by: Andrew Chisholm Received: 07/20/04 MIT, Cambridge MA, USA ----------------------------------------------------------------------------- Strain: CB4734 Species: C. elegans Genotype: tra-2(e1095)/+ II; fog-2(q71) V; xol-1(y9) X. [XX females and tra-2; fog-2; xol-1 XX males] Description: Obligate male/female strain; maintain by crossing. Anatomically normal XX females and XX males. Male/female XX strain with sex determined by tra-2(+). Reference: Strain 6 in Hodgkin (2002) PMID: 12399387. Mutagen: Outcrossed: x2 Made by: J. Hodgkin Received: 08/24/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4747 Species: Caenorhabditis elegans Genotype: dpy-20(e2017) unc-24(e138) IV; sup-33(st389) X. Description: Variable phenotypes, slightly dumpy and/or uncoordinated, especially at 25C. Grows poorly at 15C. Low fertility. sup-33(st389) is a weak amber suppressor; e2017 and e138 are amber mutations. Reference: Kondo K, et al. (1990) J Mol Biol. 1990 Sep 5;215(1):7-19. Mutagen: Outcrossed: x2 Made by: J. Hodgkin Received: 07/20/16 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4760 Species: C. elegans Genotype: fem-1(e2044) mor-2(e1125) unc-24(e158) fem-3(q20) / unc-5(e53) dpy-20(e1282) IV Description: Wild-type hermaphrodites segregating wild-type hermaphrodites, Unc-24 females, Unc Dpy hermaphrodites. Maintain by picking wild-type hermaphrodites. Deletion allele of fem-1, with unusual maternal effect. Reference: Spence AM, et al. Cell. 1990 Mar 23;60(6):981-90. Johnson CL & Spence AM.. Science. 2011 Sep 2;333(6047):1311-4. Mutagen: 32P mutagenesis of N2 Outcrossed: x3 Made by: Andrew M. Spence Received: 08/27/18 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4783 Species: Caenorhabditis elegans Genotype: mup-1(e2430) II. Description: Temperature sensitive two-fold arrest lethal. Maintain at 15C. Easily scored, at restrictive temperature they look like tiny little croissants. Mutagen: EMS Outcrossed: x Made by: Bogaert T Received: 02/09/90 DevGen NV, Zwijnaarde, Belgium ----------------------------------------------------------------------------- Strain: CB4784 Species: Caenorhabditis elegans Genotype: mup-1(e2347)/+ II. Description: Unbalanced two-fold arrest lethal. Clone 20 - 30 WT, screen F1 for presence of mup-1 homozygotes. Easily scored, they look like tiny little croissants. Mutagen: EMS Outcrossed: x Made by: Bogaert T Received: 02/09/90 DevGen NV, Zwijnaarde, Belgium ----------------------------------------------------------------------------- Strain: CB4785 Species: Caenorhabditis elegans Genotype: mup-1(e2434)/+ II. Description: Unbalanced two-fold arrest lethal. Clone 20 - 30 WT, screen F1 for presence of mup-1 homozygotes. Easily scored, they look like tiny little croissants. Mutagen: EMS Outcrossed: x Made by: Bogaert T Received: 02/09/90 DevGen NV, Zwijnaarde, Belgium ----------------------------------------------------------------------------- Strain: CB4786 Species: Caenorhabditis elegans Genotype: mup-1(e2436)/+ II. Description: Unbalanced two-fold arrest lethal. Clone 20 - 30 WT, screen F1 for presence of mup-1 homozygotes. Easily scored, they look like tiny little croissants. Mutagen: EMS Outcrossed: x Made by: Bogaert T Received: 02/09/90 DevGen NV, Zwijnaarde, Belgium ----------------------------------------------------------------------------- Strain: CB4787 Species: Caenorhabditis elegans Genotype: mup-1(e2438)/+ II. Description: Unbalanced two-fold arrest lethal. Clone 20 - 30 WT, screen F1 for presence of mup-1 homozygotes. Easily scored, they look like tiny little croissants. Mutagen: EMS Outcrossed: x Made by: Bogaert T Received: 02/09/90 DevGen NV, Zwijnaarde, Belgium ----------------------------------------------------------------------------- Strain: CB4788 Species: Caenorhabditis elegans Genotype: mup-1(e2439)/+ II. Description: Unbalanced two-fold arrest lethal. Clone 20 - 30 WT, screen F1 for presence of mup-1 homozygotes. Easily scored, they look like tiny little croissants. Mutagen: EMS Outcrossed: x Made by: Bogaert T Received: 02/09/90 DevGen NV, Zwijnaarde, Belgium ----------------------------------------------------------------------------- Strain: CB4789 Species: Caenorhabditis elegans Genotype: mup-1(e2437) dpy-10(m481) II. Description: mup-1 is a two-fold arrest lethal, suppressed in this strain by dpy-10. Mutagen: EMS Outcrossed: x Made by: Bogaert T Received: 02/09/90 DevGen NV, Zwijnaarde, Belgium ----------------------------------------------------------------------------- Strain: CB4791 Species: Caenorhabditis elegans Genotype: mup-1(e2436) dpy-10(e128) II. Description: mup-1 is a two-fold arrest lethal, suppressed in this strain by dpy-10. Mutagen: EMS Outcrossed: x Made by: Bogaert T Received: 02/09/90 DevGen NV, Zwijnaarde, Belgium ----------------------------------------------------------------------------- Strain: CB4792 Species: Caenorhabditis elegans Genotype: mup-1(e2430) dpy-10(e128) II. Description: mup-1(e2430), a ts two-fold arrest lethal, is suppressed by dpy-10. Mutagen: EMS Outcrossed: x Made by: Bogaert T Received: 02/09/90 DevGen NV, Zwijnaarde, Belgium ----------------------------------------------------------------------------- Strain: CB4793 Species: Caenorhabditis elegans Genotype: mup-1(e2430) lin-31(n301) unc-85(e1414) II. Description: mup-1(e2430) is a temperature sensitive two-fold arrest lethal. Maintain at 15C. Easily scored, at restrictive temperature they look like tiny little croissants. Muv and Unc. Mutagen: EMS Outcrossed: x Made by: Bogaert T Received: 02/09/90 DevGen NV, Zwijnaarde, Belgium ----------------------------------------------------------------------------- Strain: CB4817 Species: Caenorhabditis elegans Genotype: wDf5(e500)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUncs and dead eggs. Mutagen: EMS Outcrossed: x Made by: Joel Rothman Received: 01/23/03 University of California, Santa Barbara CA, USA ----------------------------------------------------------------------------- Strain: CB4834 Species: C. elegans Genotype: tra-3(e1108); eEx24. Description: eEx24 [tra-3(+) + rol-6(su1006)]. Pick Rollers to maintain. Roller hermaphrodites producing more Rol hermaphrodites and non-Rol hermaphrodites that produce broods of 100% Tra-3 XX pseudomales. Tra-3 mutant rescued by transgenic tra-3(+); useful source of homozygous m+z- tra-3 XX hermaphrodites. Reference: Barnes & Hodgkin (1996) PMID: 8887539. Mutagen: Outcrossed: x2 Made by: T.M Barnes Received: 08/24/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4845 Species: Caenorhabditis elegans Genotype: unc-119(e2498) III. Description: Small, severely uncoordinated. Spontaneous segregant from N2/RW7000 (Bristol/Bergerac) hybrid strain. Daf-d. Mutagen: Spontaneous Outcrossed: x Made by: Dave Pilgrim Received: 02/14/94 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4851 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Wild type-slightly Unc. Tc1 pattern high copy; different from RW7000. Bergerac isolate obtained by S. Brenner from Brun group. Caenorhabditis elegans wild isolate. CB subclone of Bergerac N62 (Tc1 pattern HCB). To obtain ECA243, a sequenced isolate of this wild strain, please visit the C. elegans Natural Diversity Resource at www.elegansvariation.org. Mutagen: Outcrossed: x Made by: Received: 01/31/91 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4852 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Wild type. Low Tc1 copy number; pattern X. [Obtained from Rothamsted by S. Brenner as 'Panagrellus redivivus'. Cross fertile with C. elegans N2.] Caenorhabditis elegans wild isolate. CB subclone of N3 (Tc1 pattern X). Mutagen: Outcrossed: x Made by: Received: 01/31/91 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4853 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated from Carl Johnson's organic garden in Altadena, CA in 1974. Wild type. Low copy Tc1; pattern III. Caenorhabditis elegans wild isolate. CB subclone of GA-12 (Tc1 pattern III). To obtain ECA245, a sequenced isolate of this wild strain, please visit the C. elegans Natural Diversity Resource at www.elegansvariation.org. Mutagen: Outcrossed: x Made by: Johnson C Received: 01/31/91 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4854 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated from Carl Johnson's organic garden in Altadena, CA in 1974. Wild type. Low copy Tc1, pattern V. Caenorhabditis elegans wild isolate. CB subclone of GA-9 (Tc1 pattern V). Mutagen: Outcrossed: x Made by: Johnson C Received: 01/31/91 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4855 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: NOTE: Whole-genome analysis indicates that this stock is genotypically CB4858. Users interested in this strain are encouraged to obtain a verified CB4855-derivied strain. To obtain ECA247, a sequenced isolate of this wild strain, please visit the C. elegans Natural Diversity Resource at www.elegansvariation.org. Isolated from compost in Palo Alto, CA in 1982(?). Wild type (plg-1(e2001)). Low copy Tc1, pattern VI. Caenorhabditis elegans wild isolate CB subclone of Sta-5 (Tc1 pattern VI). Original stock isolated by T. Doniach. Mutagen: Outcrossed: x Made by: Doniach/Greenstein Received: 12/18/08 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB4856 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated from a pineapple field in Hawaii in 1972 by L. Hollen. Wild type. Low copy Tc1; pattern IX. C. elegans wild isolate. CB subclone of HA-8 (Tc1 pattern IX). See also WBPaper00005369. [NOTE (4-2014): Users reported abnormalities in CB4856 in late 2013 and early 2014. The current working stock at the CGC was thawed in 2-2014 from stock frozen in 1995.] Mutagen: Outcrossed: x Made by: Hollen L Received: 01/31/91 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4857 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated from decaying mushroom during rain in Claremont, CA in November 1972. Wild type. Low copy Tc1, pattern II. Reference WBG 10(2) 140-141 and 11(5) 60. Caenorhabditis elegans wild isolate. CB subclone of Cl2a (Tc1 pattern II). To obtain ECA249, a sequenced isolate of this wild strain, please visit the C. elegans Natural Diversity Resource at www.elegansvariation.org. Mutagen: Outcrossed: x Made by: Received: 01/31/91 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4858 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated from Caltech flowerbed in the summer of 1971 (1973??) in Pasadena, CA. Wild type. Low copy Tc1; pattern XI. Caenorhabditis elegans wild isolate. EM subclone of PA1 (Tc1 pattern XI). To obtain ECA251, a sequenced isolate of this wild strain, please visit the C. elegans Natural Diversity Resource at www.elegansvariation.org. Mutagen: Outcrossed: x Made by: Hedgecock E Received: 01/31/91 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4869 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Hermaphrodites are WT at all stages. Adult males abnormal with swollen distorted bursal anatomy. Male mating efficiency is zero. Natural mutation present in original KR314 isolate. Mutagen: Outcrossed: x2 Made by: Jonathan Hodgkin Received: 02/01/93 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4870 Species: Caenorhabditis elegans Genotype: unc-122(e2520) I. Description: Medium coiler Unc at all stages. Mutagen: EMS Outcrossed: x2 Made by: Ed Hedgecock Received: 02/01/93 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4876 Species: Caenorhabditis elegans Genotype: clk-1(e2519) III. Description: Maternally rescued. Homozygous e2519 worms coming from homozygous mothers develop slowly. Adult rhythmic behaviors are also slowed. Mutagen: EMS Outcrossed: x20 Made by: Siegfried Hekimi Received: 06/27/95 McGill University, Montreal PQ, Canada ----------------------------------------------------------------------------- Strain: CB4883 Species: Caenorhabditis elegans Genotype: let-551(e2517)/dpy-21(e428) rol-9(sc148) V. Description: Heterozygotes are WT and segregate WT, Lethals (L2/L3 arrest at 20C, some survive to sterile adults at 15C), and DpyRol. Not balanced well: get recombinants. Must maintain by picking hets at each generation and check for proper segregants in F1. Mutagen: Spontaneous from TR388 Outcrossed: x2 Made by: J. Hodgkin Received: 08/12/93 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB489 Species: Caenorhabditis elegans Genotype: dpy-2(e489) II. Description: Temperature sensitive. Left hand Roller. Recessive. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4890 Species: C. elegans Genotype: smg-2(e2008) I; tra-1(e1488) III. Description: Intersexual self-fertile XX hermaphrodites with Smg (protruding vulva) phenotype. Grows poorly, does best at 20C. Class B1 allele of tra-1 combined with smg-2(NMD), useful for selection of modifiers. Reference: Zarkower et al. (1994) PMID: 7520378 Mutagen: Outcrossed: x2 Made by: J. Hodgkin Received: 09/13/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB49 Species: Caenorhabditis elegans Genotype: unc-8(e49) IV. Description: Unc. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB4900 Species: C. elegans Genotype: tra-1(rh132) III; eDp6 (III; f). Description: Fertile hermaphrodites segregating more fertile hermaphrodites and sterile Class A10 tra-1(rh132) XX homozygotes, which have hermaphrodite non-gonadal anatomy and male gonads; Smg (NMD)-sensitive. Reference: Zarkower et al. (1994) PMID: 7520378 Mutagen: Outcrossed: x2 Made by: J. Hodgkin Received: 08/31/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4905 Species: C. elegans Genotype: tra-2(e1403) II. Description: Weak Tra-2 allele, homozygous self-fertile XX. Slightly masculinized hermaphrodite, adults lack tail spike. Reference: Hodgkin & Barnes (1991) PMID: 1684664. Mutagen: Outcrossed: x3 Made by: J. Hodgkin Received: 08/31/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB491 Species: Caenorhabditis elegans Genotype: sma-3(e491) III. Description: Small. Recessive. Males abnormal-abnormal spicules. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4932 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Wild isolate with unique Tc1 pattern. Bor phenotype. Isolated by P.S. Grewal before 1991, from mushroom farm near Taunton, England. Sent to Ann Burnell in 1991. Sent from Burnell to J. Hodgkin in Jan. 1992. Sent to CGC in Jan. 1997. Caenorhabditis elegans wild isolate. Mutagen: Outcrossed: x Made by: P.S. Grewal Received: 01/21/97 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4949 Species: Caenorhabditis elegans Genotype: dpy-26(n199) IV; eDp26 X. Description: dpy-26(n199) is XO viable, XX lethal. eDp26 is a duplication of the left end of X, attached to X in an inverted orientation. Strain gives non-Dpy Egl hermaphrodites, intersexes, males and dead eggs. Mutagen: Outcrossed: x Made by: Received: 05/03/95 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4950 Species: Caenorhabditis elegans Genotype: eDp26 X. Description: eDp26 is a duplication of the left end of X, attached to X in an inverted orientation. CB4950 is homozygous for eDp26, which is XX viable and XO lethal. XX hermaphrodites are slightly small and slightly Egl. Mutagen: Outcrossed: x Made by: Received: 05/03/95 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4951 Species: Caenorhabditis elegans Genotype: spe-12(hc76) I; him-8(e1489) IV. Description: Male/female strain which must be maintained by mating. spe-12(hc76) prevents self-fertility in hermaphrodites but does not impair spermatogenesis in males. Presence of him-8(e1489) increases male frequency. Mutagen: Outcrossed: x Made by: Jonathan Hodgkin Received: 12/07/98 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB4952 Species: C. elegans Genotype: tra-2(e2541) II. Description: Hypomorphic allele. Homozygous XX animals are masculinized; have hermaphrodite gonad, abnormal male non-gonadal anatomy; self-fertile. Mutagen: EMS Outcrossed: x2 Made by: J. Hodgkin Received: 08/31/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5 Species: Caenorhabditis elegans Genotype: unc-7(e5) X. Description: Unc. Moves backward better than forward, kinker in forward movement. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB5009 Species: Caenorhabditis elegans Genotype: let-552(e2542)/dpy-25(e817) II. Description: At 20C or 25C heterozygotes are semi-Dpy and segregate semi-Dpys (heterozygotes), Dpys (dpy-25/dpy-25) and animals which arrest as Dpyish young larvae (let-552/let-552). Maintain by picking semi-Dpy. At 15C it is difficult to distinguish between let-552 homozygotes and dpy-25 homozygotes (e817 is inviable at 15C). Mutagen: Outcrossed: x Made by: Jonathan Hodgkin Received: 01/06/94 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: CB502 Species: Caenorhabditis elegans Genotype: sma-2(e502) III. Description: Small. Recessive. Male spicules abnormal. M-MATING-NO SUCCESS. Synthetic lethal common. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5020 Species: Caenorhabditis elegans Genotype: gro-3(e2556) I. Description: Slow growth and movement. Egl. Somewhat Uncoordinated. Maternal effect: m+z- homozygotes have no obvious phenotype. Mutagen: Spontaneous Outcrossed: x3 Made by: J. Hodgkin Received: 03/13/97 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5023 Species: C. elegans Genotype: tra-2(e2046e253) unc-4(e120) / + + II; dpy-26(n199) IV. [XO females, dpy-26 XO males] Description: Obligate XO male/female strain, propagate by crossing. Low fertility. Fertile XO females and XO males; inviable XX embryos and larvae. Reference: Strain 7 Hodgkin (2002) PMID: 12399387. Mutagen: Outcrossed: x1 Made by: J. Hodgkin Received: 08/31/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5035 Species: C. elegans Genotype: tra-1(e1575 e1816 e1834) III; eDp25[tra-1(e1575 e1816)] (III; f). Description: Male/female strain, propagate by crossing. XX females and tra-1 XX males. Low fertility. Fertile females and fertile males; male-biased sex ratio. Strain with sex determined by a feminizing fragment chromosome (LGIIIR); unusual sex ratio. Reference: Strain 15 in Hodgkin (2002) PMID: 12399387. Mutagen: Outcrossed: x2 Made by: J. Hodgkin Received: 09/13/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5042 Species: C. elegans Genotype: lon-2(e678)^lon-2(e678) X. Description: Attached X chromosome line. Long hermaphrodites with low fertility, producing some X^X X^X dead eggs. May spontaneously revert to unattached lon-2 XX. Reference: Hodgkin & Albertson (1995) PMID: 8647390. Mutagen: Outcrossed: x2 Made by: J. Hodgkin Received: 08/31/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB51 Species: Caenorhabditis elegans Genotype: unc-13(e51) unc-122(n2916) I. Description: Unc. See WBPaper00003781 regarding the unc-122 mutation. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB5101 Species: Caenorhabditis elegans Genotype: dpy-26(n199) IV; eEx36. Description: eEx36 [F16E1 + rol-6(su1006)]. eEx36 carries multiple copies of fox-1, which confers a Xol phenotype and pRF4 which confers a Rol phenotype. dpy-26(n199) is XO viable and XX lethal. Strain consists mostly of Rol hermaphrodites and non-Rol males, all XO. Mutagen: Outcrossed: x Made by: Received: 05/03/95 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5133 Species: C. elegans Genotype: tra-1(e1732) III; eDp25[tra-1(e1575 e1816)] (III; f). Description: Male/female strain, propagated by crossing. eDp25 carries tra-1 gain-of-function allele. Anatomically normal XX females, tra-1(e1732) XO males, and intersexual tra-1(e1732) XX and e1732; eDp25 XO. References: Hodgkin (1987) PMID: 3428597. Zarkower et al. (1994) PMID: 7520378. Mutagen: Outcrossed: x2 Made by: J. Hodgkin Received: 09/13/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5140 Species: Caenorhabditis elegans Genotype: gro-2(e2442) IV. Description: Slow growth. Maternal rescue: m+z- homozygotes have no obvious phenotype. Mutagen: Bergerac Outcrossed: x2 Made by: Barnes/Hodgkin Received: 03/13/97 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5145 Species: Caenorhabditis elegans Genotype: vab-14(e2610) X. Description: Hermaphrodites have abnormal truncated or blebbed tails, pale appearance, some excretory cell abnormalities, especially in adults. Males have variably abnormal tails, some are able to mate. Mutagen: EMS Outcrossed: x2 Made by: J. Hodgkin Received: 08/11/98 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5161 Species: Caenorhabditis brenneri Genotype: Caenorhabditis brenneri. Description: Male-female strain. Reference WBG 9(3) 121. Isolated from sugar cane in Trinidad by D.J. Hunt (Commonwealth Institute of Parasitology). [7/7/93: Scott Baird and David Fitch question whether this strain is really C. remanei.] [7/95: Not cross-fertile with the authentic C. remanei isolated by Walter Sudhaus.] Previously called C. remanei by the CGC. Walter Sudhaus has tentatively described this strain as Caenorhabditis sp.; this description is not offical and is contigent upon its being published. See WBPaper00002633 and WBPaper00003993. sp. 4 in Kiontke and Sudhaus Wormbook Ecology chapter. Mutagen: Outcrossed: x Made by: Andras Fodor Received: 04/04/88 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5166 Species: Caenorhabditis elegans Genotype: dif-1(e2562) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Uncs, dead eggs, and WT (dif-1/dif-1). The WT animals give 100% dead eggs. dif-1 is a maternal effect embryonic lethal gene and e2562 is a putative null allele. Mutagen: UV Outcrossed: x Made by: Julie Ahringer Received: 08/16/95 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB518 Species: Caenorhabditis elegans Genotype: bli-5(e518) III. Description: Blistered cuticle. Small. Bursae abnormal. Males abnormal. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 09/01/77 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5190 Species: C. elegans Genotype: tra-2(e2020) II; tra-1(e1099)/+ III; xol-1(y9) X. Description: Heterozygous. Maintain by picking fertile hermaphrodites. Stable XX male/female strain (fertile XX females and low fertility XX males); males sire only female progeny. Reference: Strain 3 in Hodgkin (2002) PMID: 12399387. Mutagen: Outcrossed: x3 Made by: J. Hodgkin Received: 08/31/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5200 Species: C. elegans Genotype: smg-2(e2008) I; tra-1(e2272) III. Description: Self-fertile hermaprodite or female. Protruding vulva. tra-1(e2272) XX homozygotes are sterile with abnormal gonads; e2272 mutation is partly suppressed by NMD (smg-2(e2008)), hence double mutants are fertile. Reference: Zarkower et al. (1994) PMID: 7520378. Mutagen: EMS Outcrossed: x2 Made by: J. Hodgkin Received: 08/31/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5203 Species: Caenorhabditis elegans Genotype: plg-1(e2001) III. Description: Hermaphrodites are WT. Males form copulatory plug when mating. Weaker than CB3855 males. Mutagen: Outcrossed: x>6 Made by: Jonathan Hodgkin Received: 06/04/01 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5213 Species: C. elegans Genotype: tra-2(e2272e2624) II. Description: Intragenic partial revertant of tra-1(e2272). Fertile hermaphrodites or females. Reference: Zarkower et al. (1994) PMID: 7520378. Mutagen: EMS Outcrossed: x2 Made by: J. Hodgkin Received: 08/31/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB522 Species: Caenorhabditis elegans Genotype: unc-29(e522) I. Description: Mutagen: EMS Outcrossed: x0 Made by: S. Brenner Received: 03/01/85 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB524 Species: Caenorhabditis elegans Genotype: unc-70(e524) V. Description: Unc. Semi-dominant at 25C. M-MATING+++ 10-30%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5265 Species: C. elegans Genotype: sup-1(e995e2636) III; unc-17(e245) IV; xol-1(y9) X. Description: Severely uncoordinated coiler, slow growing. Useful strain for selecting non-Sup-1 suppressors of unc-17(e245). Reference: Mathews et al. (2012) PMID: 23051648. Mutagen: Outcrossed: x2 Made by: J. Hodgkin Received: 08/31/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5281 Species: Caenorhabditis elegans Genotype: air-2(e2631)/dpy-5(e61) I; him-8(e1489) IV. Description: WT hermaphrodites segregate WT hermaphrodites and males, Dpy hermaphrodites and males and Stu (Sterile and Uncoordinated) hermaphrodites and males. Mutagen: EMS Outcrossed: x2 Made by: J. Hodgkin Received: 08/10/99 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5300 Species: Caenorhabditis elegans Genotype: fem-1(hc17) IV; xol-1(y9) X. Description: WT hermaphrodite if grown at 15-20C. WT females if grown at 25C. When crossed, these females yield only XX viable hermaphrodite progeny, all XO progeny are dead. Mutagen: Outcrossed: x Made by: J. Hodgkin Received: 08/11/98 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5310 Species: C. elegans Genotype: unc-4(e120) II; lon-2(e678)^lon-2(e678) X. Description: Attached X strain with autosomal unc-4 marker, which facilitates test-crosses. Uncoordinated Long hermaphrodites, reduced fertility with many X^X X^X unhatched eggs. Reference: Hodgkin & Albertson (1995) PMID: 8647390. Mutagen: Outcrossed: x2 Made by: J. Hodgkin Received: 08/31/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5311 Species: C. elegans Genotype: egl-26(e1952e2650) II; him-8(e1489) IV. Description: Partial intragenic revertant. Weak Egl phenotype, less severe than egl-26(e1952). Reference: Hodgkin (1986) PMID: 3770465. Mutagen: Spontaneous Outcrossed: x2 Made by: J. Hodgkin Received: 08/31/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5330 Species: Caenorhabditis elegans Genotype: vab-12(dx25) III; him-8(e1489) IV. Description: Vab XX hermaphrodites and XO males, viable at all temperatures. Adult hermaphrodite tail spike is invariably shortened and/or vacuolated, often also abnormal in larvae. Possible excretory cell abnormalities. Rays of adult male tail variably abnormal, other structures normal; males can mate. Mutagen: EMS Outcrossed: x2 Made by: E Lambie & J Hodgkin Received: 02/05/98 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5337 Species: C. elegans Genotype: sma-8(e2656) V. Description: Small, blunt rounded nose; heterozygous e2656/+ are animals similar, XO males can mate. Molecularly and phenotypically different from sma-8(e2111). Mutagen: EMS Outcrossed: x3 Made by: J. Hodgkin Received: 08/31/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5348 Species: Caenorhabditis elegans Genotype: mrt-2(e2663) III. Description: Unable to propagate indefinitely: lines become sterile from F10-F28, with short telomeres and fused chromosomes. Hypersensitive to X-irradiation; weak Him phenotype; strong Him phenotype in later generations resulting from X-A fusions. Cross once or twice, freeze down many F2 mrt-2 plates, and go back to these plates every two months for fresh a mrt-2 line. A BstN1 RFLP makes the mrt-2 mutation easy to track. Mutagen: EMS Outcrossed: x2 Made by: Shawn Ahmed Received: 04/18/00 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5362 Species: Caenorhabditis elegans Genotype: tra-2(ar221) II; xol-1(y9) X. Description: ar221 is a tra-2 ts allele. Strain will grow at 15C as Egl hermaphrodites. When grown at 25C, all animals mature into X males, many of which are fertile. Good source of pure XX mating males. ar221 isolated by Jane Hubbard. Mutagen: Outcrossed: x Made by: J. Hodgkin Received: 08/11/98 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5365 Species: C. elegans Genotype: tra-3(bn75) IV; him-5(e1490) V. Description: Him. Temperature-sensitive: infertile above 20C. Variably masculinized XX hermaphrodites segregating normal XO males. Stronger XX masculinization than null alleles of tra-3. Reference: Francis et al. (1995) PMID: 7713420. Mutagen: Outcrossed: x2 Made by: J. Hodgkin Received: 09/13/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5378 Species: Caenorhabditis elegans Genotype: unc-42(e270) let-560(e2658) V/nT1 (IV;V). Description: let-560(e2658) was a spontaneous mutation in strain DH424. let-560 homozygotes are late embryonic lethal. Strain segregates WT, Vul and dead eggs. Mutagen: Outcrossed: x Made by: J. Hodgkin Received: 08/11/98 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB538 Species: Caenorhabditis elegans Genotype: unc-1(e538) X. Description: Recessive. Kinker Unc. Mutagen: Outcrossed: x Made by: Received: 08/02/94 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB5380 Species: Caenorhabditis elegans Genotype: fox-1(e2643) X. Description: fox-1(e2643) is a 1.2 kb deletion of fox-1; functional null. Hermaphrodites and males are WT in gross phenotype, slightly abnormal in hermaphrodite fertility and male mating. Synergistic masculinizing effects with some X chromosome deletions. Mutagen: Tc1 Outcrossed: x>5 Made by: M. Skipper Received: 04/05/99 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB540 Species: Caenorhabditis elegans Genotype: unc-68(e540) V. Description: Unc. See also WBPaper00002536. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB541 Species: Caenorhabditis elegans Genotype: unc-71(e541) III. Description: Unc. Recessive. M-MATING+++ 10-30%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 08/01/77 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5414 Species: Caenorhabditis elegans Genotype: srd-1(eh1) II. Description: EMS-induced deletion from -583 to +1017, including first two exons and part of third exon. Probable null. No obvious phenotype. Mutagen: EMS Outcrossed: x5 Made by: D. Hughes/M. Bickle Received: 04/05/99 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5423 Species: Caenorhabditis elegans Genotype: him-17(e2707) V. Description: Him. Sterile at 25C. Weak allele, R862stop. Mutagen: EMS Outcrossed: x3 Made by: J. Hodgkin Received: 02/28/07 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5443 Species: Caenorhabditis elegans Genotype: bus-4(e2693) IV. Description: Resistant to infection by Microbacterium nematophilum (no tail swelling). Slightly small. Mutagen: EMS Outcrossed: x1 Made by: JA Hodgkin Received: 09/08/05 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5475 Species: C. elegans Genotype: her-1(e1518) V; sdc-2(y15) X. Description: Obligate XO hermaphrodite. Low fertility, segregating many dead XX and nullo-X zygotes. Double mutant combining two null or near-null mutations. Reference: van den Berg MCW, et al. Genetics. 2006 Jun;173(2):677-83. doi: 10.1534/genetics.106.056093. PMID: 16582430. Mutagen: EMS Outcrossed: x3 Made by: J. Hodgkin Received: 11/05/24 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5495 Species: C. elegans Genotype: bus-10 & ZK596.4 & ZK596.5(e2715) IV. Description: Viable, Bus, resistant to Leucobacter Verde2, hypersensitive to Leucobacter Verde1. e2715 is a small deficiency (3191 bp) which removes all of the bus-10 exons and internal ncRNA genes ZK596.4 & ZK596.5. Reference: O'Rourke et al (in preparation). Mutagen: mut-7 Outcrossed: x3 Made by: J Hodgkin Received: 10/10/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB55 Species: Caenorhabditis elegans Genotype: unc-2(e55) X. Description: Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB5535 Species: Caenorhabditis elegans Genotype: hda-1(e1795) V/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Unc, non-Unc Sterile Gon, and dead eggs. Mutagen: EMS Outcrossed: x4 Made by: J. Hodgkin Received: 02/28/07 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5542 Species: Caenorhabditis elegans Genotype: dpy-6(e2762) X. Description: Dumpy. e2760/0 Dpy males are able to mate (ME 1/2), in contrast to other dpy-6 alleles. mrt-2 induced. Mutagen: Outcrossed: x3 Made by: S. Ahmed/J. Hodgkin Received: 12/04/00 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5584 Species: Caenorhabditis elegans Genotype: mIs12 II. Description: mIs12 [myo-2p::GFP + pes-10p::GFP + F22B7.9p::GFP] II. Hermaphrodites expressing compound GFP reporter (see PD4790). Strong pharyngeal muscle expression, easily scored by GFP dissecting scope. mIs12 is tightly linked to unc-4 II, and not to LG III or IV as previously reported. mIs12 homozygous males mate well (ME3). Mutagen: Outcrossed: x1 Made by: J. Hodgkin Received: 12/04/00 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5590 Species: C. elegans Genotype: her-1(e1518) sdc-3(y52) V; xol-1(y9) X. [XX hermaphrodites, her-1/+ sdc-3/sdc-3; xol-1 XX males] Description: Maintain by crossing males and hermaphrodites; may revert to purely hermaphrodite line. Fertile hermaphrodites and fertile males. Obligate XX strain with sex determined by her-1 V. Reference: Strain 9 in Hodgkin (2002) PMID: 12399387. Mutagen: Outcrossed: x2 Made by: J. Hodgkin Received: 08/31/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5595 Species: Caenorhabditis elegans Genotype: unc-85(e1414) II; him-8(e1489) IV. Description: Unc hermaphrodites and males. Mate have variably abnormal tail anatomy. ME 0. Mutagen: Outcrossed: x1 Made by: J. Hodgkin Received: 12/04/00 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5600 Species: Caenorhabditis elegans Genotype: ccIs4251 I; him-8(e1489) IV. Description: ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. Superficially WT hermaphrodites and males expressing GFP in nuclei and mitochondria of body wall muscles. Fluorescent body wall muscle nuclei can be seen by dissecting microscope with epifluoresence optics. Males mate poorly (ME 1/2). ccIs4251 mapped to LGI, + 2.5. Mutagen: Outcrossed: x1 Made by: J. Hodgkin Received: 12/04/00 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5602 Species: Caenorhabditis elegans Genotype: vhl-1(ok161) X. Description: vhl-1 deletion. Constitutive HIF-1 in normoxia, slow growth and reduced brood size. See also WBPaper00005962. Mutagen: TMP Outcrossed: x5+ Made by: Barstead/Hodgkin Received: 11/26/01 Wellcome Trust Centre for Human Genetics, Oxford, England ----------------------------------------------------------------------------- Strain: CB5609 Species: Caenorhabditis elegans Genotype: bus-1(e2678) V. Description: Resistant to infection by Microbacterium nematophilum (no tail swelling). Mutagen: EMS Outcrossed: x2 Made by: JA Hodgkin Received: 09/08/05 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5610 Species: Caenorhabditis elegans Genotype: bus-2(e2687) IV. Description: Resistant to infection by Microbacterium nematophilum (no tail swelling). Mutagen: EMS Outcrossed: x2 Made by: JA Hodgkin Received: 09/08/05 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5611 Species: Caenorhabditis elegans Genotype: bus-3(e2696) I. Description: Resistant to infection by Microbacterium nematophilum (no tail swelling). Mutagen: EMS Outcrossed: x2 Made by: JA Hodgkin Received: 09/08/05 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5612 Species: Caenorhabditis elegans Genotype: bus-5(e2688) X. Description: Resistant to infection by Microbacterium nematophilum (no tail swelling). Slightly skiddy movement, some cuticle fragility. Mutagen: EMS Outcrossed: x2 Made by: JA Hodgkin Received: 09/08/05 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5613 Species: Caenorhabditis elegans Genotype: bus-6(e2691) V. Description: Resistant to infection by Microbacterium nematophilum (no tail swelling). Mutagen: EMS Outcrossed: x3 Made by: JA Hodgkin Received: 09/08/05 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5614 Species: C. elegans Genotype: bus-5(e2701) X. Description: Bus (M. nematophilum resistant), bleach-sensitive, non-Bah (Yersinia sensitive). e2701 is an intermediate strength missense allele, S167N (YAASKAA>YAANKAA). Reference: O'Rourke et al (in preparation). Mutagen: EMS Outcrossed: x3 Made by: J Hodgkin Received: 10/10/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5617 Species: Caenorhabditis elegans Genotype: bus-10(e2702) IV. Description: Resistant to infection by Microbacterium nematophilum (no tail swelling). Mutagen: EMS Outcrossed: x2 Made by: JA Hodgkin Received: 09/08/05 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5635 Species: Caenorhabditis elegans Genotype: ptr-15(e2710) V. Description: Resistant to infection by Microbacterium nematophilum (no tail swelling). Abnormal lectin staining. ptr-15 also known as bus-13. Mutagen: EMS Outcrossed: x3 Made by: JA Hodgkin Received: 09/08/05 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5636 Species: Caenorhabditis elegans Genotype: bus-12(e2740) IV. Description: Resistant to infection by Microbacterium nematophilum (no tail swelling). Mutagen: mut-7 Outcrossed: x3 Made by: JA Hodgkin Received: 09/08/05 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5638 Species: C. elegans Genotype: sup-34(e2227)/+ I; tra-3(e1107) IV; xol-1(y9) X. [XX hermaphrodites and tra-3; xol-1 XX males] Description: Male/hermaphrodite strain, propagate by crossing. Fertile XX hermaphrodites and fertile XX males. Obligate XX strain with sex determined by amber-suppressing tRNA, sup-34. Reference: Strain 14 in Hodgkin (2002) PMID: 12399387. Mutagen: Outcrossed: x2 Made by: J. Hodgkin Received: 09/13/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB566 Species: Caenorhabditis elegans Genotype: unc-34(e566) V. Description: Unc. Mutagen: EMS Outcrossed: x1 Made by: S. Brenner Received: 06/03/93 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5661 Species: C. elegans Genotype: srf-2(e2771) I. Description: Surface abnormal, resistant to infection by Microbacterium nematophilum. Reference: Gravato-Nobre et al. (2005) PMID: 16079230. Mutagen: Tc1 Outcrossed: x3 Made by: M. J. Gravato-Nobre Received: 08/31/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5664 Species: C. elegans Genotype: dpy-31(e2770) III; sqt-3(e2809) V. Description: Dumpy (partially-suppressed Dpy-31). Sqt-3 mediated suppression of dpy-31 lethality. Reference: Novelli et al. (2004) PMID: 15579684. Mutagen: EMS Outcrossed: x2 Made by: J. Novelli Received: 08/31/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB5680 Species: Caenorhabditis elegans Genotype: bus-16(e2802) I. Description: Resistant to infection by Microbacterium nematophilum (no tail swelling). Skiddy movement; frequent vulval rupture; bleach sensitive; hypersensitive to drugs. Abnormal lectin staining. Mutagen: EMS Outcrossed: x2 Made by: Hodgkin/Whittington Received: 09/08/05 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB569 Species: Caenorhabditis elegans Genotype: unc-54(e569) I. Description: Paralyzed Unc. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 08/01/80 Baylor College of Medicine, Houston, TX ----------------------------------------------------------------------------- Strain: CB5699 Species: Caenorhabditis elegans Genotype: bus-15(e2709) IV. Description: Abnormal response to infection by Microbacterium nematophilum (no tail swelling). Abnormal lectin staining. Mutagen: EMS Outcrossed: x2 Made by: JA Hodgkin Received: 09/08/05 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB57 Species: Caenorhabditis elegans Genotype: unc-14(e57) I. Description: Unc. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB572 Species: Caenorhabditis elegans Genotype: unc-33(e572) IV. Description: Paralyzed Unc. Small. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB576 Species: Caenorhabditis elegans Genotype: unc-54(e576) I. Description: Slow movement. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 08/01/80 Baylor College of Medicine, Houston, TX ----------------------------------------------------------------------------- Strain: CB587 Species: Caenorhabditis elegans Genotype: unc-69(e587) III. Description: Unc. Recessive. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6036 Species: Caenorhabditis elegans Genotype: him-17(e2806) V. Description: Him. Sterile at 25C. Weak allele, M223I. Mutagen: EMS Outcrossed: x4 Made by: J. Hodgkin Received: 02/28/07 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6037 Species: Caenorhabditis elegans Genotype: bus-18(e2795) V. Description: Resistant to infection by Microbacterium nematophilum (no tail swelling). Small, skiddy movement; slow-growing; frequent vulval rupture; bleach sensitive, hypersensitive to drugs. Abnormal lectin staining. Mutagen: EMS Outcrossed: x3 Made by: Hodgkin/Whittington Received: 09/08/05 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6038 Species: C. elegans Genotype: tax-4(e2861) III. Description: Chemotaxis-defective; partly resistant to bacterial infection by Microbacterium nematophilum (Bus phenotype) and Leucobacter Verde2; abnormal surface. Reference: Yook & Hodgkin (2007) PMID: 17151260. Mutagen: MosSCI Outcrossed: x3 Made by: K. J. Yook Received: 09/13/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6055 Species: Caenorhabditis elegans Genotype: bus-8B(e2698) X. Description: Resistant to infection by Microbacterium nematophilum (no tail swelling). Slightly skiddy, fragile cuticle, bleach sensitive, hypersensitive to drugs. Abnormal lectin staining. Mutagen: EMS Outcrossed: x2 Made by: JA Hodgkin Received: 09/08/05 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6064 Species: Caenorhabditis elegans Genotype: bus-14(e2779) V. Description: Abnormal response to infection by Microbacterium nematophilum (no tail swelling). Mutagen: mut-7 Outcrossed: x3 Made by: MJ Gravato-Nobre Received: 09/08/05 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6081 Species: Caenorhabditis elegans Genotype: bus-17(e2800) X. Description: Resistant to infection by Microbacterium nematophilum (no tail swelling). Skiddy movement; bleach sensitive; hypersensitive to drugs. Abnormal lectin staining. Mutagen: EMS Outcrossed: xx3 Made by: Hodgkin/Whittington Received: 09/08/05 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6088 Species: Caenorhabditis elegans Genotype: egl-9(sa307) hif-1(ia4) V. Description: Mutagen: Outcrossed: x Made by: Jonathan Hodgkin Received: 01/19/05 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6090 Species: Caenorhabditis elegans Genotype: hif-1(ia4) V; vhl-1(ok161) X. Description: Mutagen: Outcrossed: x Made by: Jonathan Hodgkin Received: 01/19/05 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB61 Species: Caenorhabditis elegans Genotype: dpy-5(e61) I. Description: Strong dumpy; early larvae non-Dpy. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB611 Species: Caenorhabditis elegans Genotype: unc-23(e611) V. Description: Head abnormal. Variable expression. M-MATING+++ 10-30%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB6116 Species: Caenorhabditis elegans Genotype: egl-9(sa307) V; vhl-1(ok161) X. Description: Egg-laying defective. Slow growing. Mutagen: Outcrossed: x Made by: J. Hodgkin Received: 02/28/07 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6121 Species: C. elegans Genotype: eT5 (X; III); fog-2(q71) V females and eT5 (X; III) / eT1(III; V); fog-2 / eT1 (fog-2 V; III) males Description: Male/female strain, propagate by crossing. Females are eT5 (III;X); fog-2(q71) V. Males are eT5 (III;X) / eT1(III;V); fog-2 / eT1 (fog-2 III;V). Strain with multichromosomal sex determination. Reference: Strain 21 in Hodgkin (2002) PMID: 12399387. Mutagen: Outcrossed: x2 Made by: J. Hodgkin Received: 09/13/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6129 Species: C. elegans Genotype: dpy-27(rh18) III; fem-1(hc17) IV. [4A;3X] Description: Temperature-sensitive: Maintain at 15C. Stable, temperature-sensitive tetraploid strain: fertile 4A;3X hermaphrodites and 4A;2X males at 15C; fertile 4A;3X females and feminized sterile 4A;2X males at 25C. Reference: Hodgkin (2002) PMID: 12399387. Mutagen: EMS Outcrossed: x2 Made by: J Hodgkin Received: 10/04/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6144 Species: C. elegans Genotype: dpy-31(e2770) III; eEx512. Description: eEx512 [dpy-31(+) + sur-5p::GFP]. Pick GFP+ to maintain. Lethal dpy-31 mutant rescued by array containing dpy-31(+) 6.5 kb fragment. Reference: Novelli et al. (2004) PMID: 15579684. Mutagen: Outcrossed: x2 Made by: J. Novelli Received: 09/13/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6147 Species: Caenorhabditis elegans Genotype: bus-8B(e2882) X. Description: Viable allele. Hypersensitive to many drugs. Mutagen: Outcrossed: x3 Made by: J. Hodgkin Received: 08/29/08 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6177 Species: C. elegans Genotype: bus-8B(e2883) X. Description: Viable slightly dumpy hermaphrodites, drug and bleach sensitive, resistant to M. nematophilum (Bus phenotype) and Leucobacter Verde1, hypersensitive to Leucobacter Verde1. References: Partridge et al. (2008) PMID: 18395708. Hodgkin et al. (unpublished, in preparation). Mutagen: EMS Outcrossed: x3 Made by: J. Hodgkin Received: 09/13/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6193 Species: Caenorhabditis elegans Genotype: bus-8B(e2885) X. Description: Viable allele. Hypersensitive to many drugs. Mutagen: Outcrossed: x3 Made by: J. Hodgkin Received: 08/29/08 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB620 Species: Caenorhabditis elegans Genotype: lin-18(e620) X. Description: Some hermaphrodites (<50%) have single small protrusion posterior to vulva, occassional vulval rupture. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 07/01/86 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6208 Species: Caenorhabditis elegans Genotype: bus-8B(e2887) X. Description: Cold-sensitive lethal allele. Viable at >18C. Strongly hypersensitive to many drugs. Mutagen: Outcrossed: x3 Made by: J. Hodgkin Received: 08/29/08 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6216 Species: C. elegans Genotype: bus-8B(e2887) X; eEx541. Description: eEx541 [bus-8(+) + sur-5p::GFP]. Pick GFP+ to maintain. Nearly inviable, severely dumpy bus-8 mutant rescued by array. Reference: Partridge et al. (2008) PMID: 18395708. Mutagen: Outcrossed: x1 Made by: F. Partridge Received: 09/13/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB623 Species: Caenorhabditis elegans Genotype: unc-42(e623) V. Description: Unc. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6233 Species: C. elegans Genotype: dpy-17(e2898) dpy-31(e2770) III. Description: Viable dumpy. Suppressor allele of dpy-17: lethality of dpy-31(e2770) suppressed by e2898. Reference: Novelli et al. (2006) PMID: 16452136. Mutagen: EMS/ENU Outcrossed: x2 Made by: J Novelli Received: 10/04/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6234 Species: C. elegans Genotype: dpy-17(e2899) dpy-31(e2770) III. Description: Weak dumpy. Lethal dpy-31 mutation suppressed by dpy-17 mutation. Reference: Novelli et al. (2006) PMID: 16452136. Mutagen: EMS Outcrossed: x2 Made by: J. Novelli Received: 09/13/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6246 Species: C. elegans Genotype: sqt-3(e2911) V. Description: Slightly dumpy. Variable cold-sensitive lethal (poor viability at 15C). Unusual missense allele (D297G) of sqt-3, suppresses dpy-31 lethality. Reference: Novelli et al. (2006) PMID: 16452136. Mutagen: EMS Outcrossed: x2 Made by: J. Novelli Received: 09/13/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6253 Species: C. elegans Genotype: bus-8B(e2992) X; eEx541. Description: eEx541 [bus-8(+) + sur-5p::GFP]. Pick GFP+ to maintain. bus-8(e2892) animals are viable on Leucobacter Verde1, unlike most other bus-8 mutants. References: Partridge et al. (2008) PMID: 18395708. Stroud et al (in preparation). Mutagen: EMS Outcrossed: x2 Made by: F Partridge Received: 10/04/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6335 Species: Caenorhabditis elegans Genotype: dpy-31(e2919) III; sqt-3(e2906) V. Description: Homozygous viable dpy. dpy-31(e2919) homozygotes are almost inviable, but lethality is efficiently suppressed by sqt-3(e2906). Reference: Novelli J, et al., Genetics. 2004 Nov;168(3):1259-73. Mutagen: Outcrossed: x1 Made by: J. Novelli Received: 01/03/11 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6338 Species: Caenorhabditis elegans Genotype: bus-19(e2912) V. Description: Small, skiddy movement, drug-sensitive, fragile cuticle, Bus (resistant to infection by Microbacterium nematophilum). Mutagen: Mos1 transposon Outcrossed: x3 Made by: Karen Yook Received: 04/10/07 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6347 Species: Caenorhabditis elegans Genotype: dpy-18(e364) III; hif-1(ia4) V. Description: Dpy. Mutagen: Outcrossed: x Made by: J. Hodgkin Received: 02/28/07 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6430 Species: Caenorhabditis elegans Genotype: sqt-3(e2924) V. Description: Squat, dumpy at 15C. Inviable at higher temperatures. Deletion of most of sqt-3 coding sequence. Null by antibody staining. Mutagen: EMS Outcrossed: x3 Made by: J. Novelli Received: 08/15/06 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB644 Species: Caenorhabditis elegans Genotype: unc-62(e644) V. Description: Unc. Recessive. Bursae abnormal. Males abnormal. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 09/01/77 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6449 Species: C. elegans Genotype: sqt-3(e2909) V. Description: Weak dumpy. Variable tail abnormal at 20C or higher. Cold-sensitive: most arrest as pretzel-stage embryos, with some escapers arresting as severely distorted L1 larvae at 15. Unusual missense allele (K280E) of sqt-3, cold-sensitive lethal and suppressor of dpy-31 lethality. Reference: Novelli et al. (2006) PMID: 16452136. Mutagen: EMS Outcrossed: x3 Made by: J. Novelli Received: 09/13/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6452 Species: C. elegans Genotype: dpy-25(e817) II; xol-1(y9) X. Description: Severe dumpy; dominant, hence crossing with wild-type males yields dumpy hermaphrodites and dead eggs (dpy-25/+; xol-1/0). Mutagen: Outcrossed: x2 Made by: J Hodgkin Received: 10/04/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6453 Species: C. elegans Genotype: dpy-31(e2770) unc-119(ed3) III; eIs101. Description: eIs101 [dpy-17(E301K) + unc-119(+)]. Weakly dumpy, non-Unc. dpy-31 lethality suppressed by integrated dpy-17(gf) transgene. Reference: Novelli et al. (2006) PMID: 16452136. Mutagen: Outcrossed: x2 Made by: J Novelli Received: 10/04/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB648 Species: Caenorhabditis elegans Genotype: vab-3(e648) X. Description: Notched head. Recessive. 100% Penetrance. Male spicules abnormal. M-MATING-NO SUCCESS. See also WBPaper00002236. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 06/01/80 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6503 Species: C. elegans Genotype: bgIs312 I; him-8(e1489) IV; bus-17(e2923) X. Description: bgIs312 [pes-6::GFP]. GFP expression in excretory cell only. Bus (resistant to infection by M. nematophilum and Leucobacter Verde2), hypersensitive to Leucobacter Verde1, bleach-sensitive, drug-sensitive; Him (high incidence of males). e2923 is a Mos1 insertion in bus-17 (ZK678.8). Reference: Yook & Hodgkin (2007), Genetics 175: 681-697 Mutagen: Mos1 Outcrossed: x3 Made by: Yook KJ Received: 07/13/18 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB651 Species: Caenorhabditis elegans Genotype: unc-54(e651) I. Description: Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 09/01/80 Baylor College of Medicine, Houston, TX ----------------------------------------------------------------------------- Strain: CB652 Species: Caenorhabditis elegans Genotype: dpy-22(e652) X. Description: Unc. Dpyish. Recessive. Males lethal. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 12/01/79 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6596 Species: C. elegans Genotype: bus-19(e2964) V. Description: Weak Dpy, Gro, Skd, Daf-d, bleach sensitive. Resistant to infection by M. nematophilum (Bus). <10% larval arrest with rod-like lethality. Severe missense allele (G90E), probable null allele. Reference: Yook & Hodgkin (2007) PMID: 17151260. Mutagen: EMS Outcrossed: x2 Made by: K. J. Yook Received: 09/13/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6598 Species: C. elegans Genotype: bus-19(e2966) V. Description: Bus: resistant to M. nematophilum, Leucobacter Verde2; hypersensitive to Leucobacter Verde2. Weak allele, missense (Pro235Leu). Reference: Yook & Hodgkin (2007) PMID: 17151260. Mutagen: EMS Outcrossed: x3 Made by: K J Yook Received: 09/15/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB66 Species: Caenorhabditis elegans Genotype: unc-22(e66) IV. Description: Twitcher. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB6614 Species: Caenorhabditis elegans Genotype: egl-8(e2917) V. Description: Egg-laying defective, Bus (no tail swelling after infection by Microbacterium nematophilum). Mutagen: Mos1 transposon Outcrossed: x3 Made by: Karen Yook Received: 04/10/07 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6627 Species: Caenorhabditis elegans Genotype: srf-3(e2689) IV. Description: Surface abnormal. Resistant to infection by Microbacterium nematophilum (no tail swelling). Nonsense mutation Q89stop(UAA). Mutagen: EMS Outcrossed: x4 Made by: J. Hodgkin Received: 08/15/06 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB665 Species: Caenorhabditis elegans Genotype: unc-58(e665) X. Description: Shaker Unc. Reverts spontaneously. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6667 Species: C. elegans Genotype: bus-12(e2977) IV. Description: Bus: resistant to M. nematophilum, Leucobacter Verde2; hypersensitive to Leucobacter Verde1. Strong nonsense mutation of bus-12 (Gln123Amber); reference null allele. Reference: Gravato-Nobre et al. (2011) PMID: 20980242. Mutagen: EMS Outcrossed: x3 Made by: M J Gravato-Nobre Received: 09/15/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6689 Species: C. elegans Genotype: nit-1(tm1190) III. Description: No obvious phenotype, wildtype pathogen resistance. Reference: O’Rourke et al. (2005) PMID: 16809667. Mutagen: UV/TMP Outcrossed: x3 Made by: J Hodgkin Received: 09/15/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB669 Species: Caenorhabditis elegans Genotype: unc-52(e669) II. Description: Unc-adults paralyzed. Dystrophic body muscle. Suppressed by sup-5 and sup-7. Null allele (?). Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 08/01/82 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: CB6710 Species: C. elegans Genotype: unc-119(ed3) III; eEx650. Description: eEx650 [ilys-3p::GFP + unc-119(+)]. Pick wild-type to maintain. Transcriptional reporter transgene for ilys-3. Reference: Gravato-Nobre et al. (2016) PMID: 27525822. Mutagen: Outcrossed: x2 Made by: M J Gravato-Nobre Received: 09/15/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6712 Species: C. elegans Genotype: unc-119(ed3) III; eEx652. Description: eEx652 [ilys-1p::DsRed2 + unc-119(+)]. Pick wild-type to maintain. Transcriptional reporter for ilys-1Reference: Gravato-Nobre et al. (2016) PMID: 27525822. Mutagen: Outcrossed: x2 Made by: M J Gravato-Nobre Received: 09/15/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6725 Species: C. elegans Genotype: unc-119(ed3); eEx655. Description: eEx655 [ilys-2p::CFP; unc-119(+)]. Pick wild-type (non-Unc) to maintain. Transgenic animals carry ilys-2 transcriptional reporter. Reference: Gravato-Nobre et al. (2016) PMID: 27525822. Mutagen: Outcrossed: x2 Made by: M J Gravato-Nobre Received: 10/04/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6734 Species: C. elegans Genotype: clec-60(tm2319) II. Description: No obvious phenotype. Reference: O’Rourke et al. (2005) PMID: 16809667. Mutagen: Outcrossed: x3 Made by: J Hodgkin Received: 10/04/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6736 Species: C. elegans Genotype: clec-61(tm2566) II. Description: No obvious phenotype, apparently wildtype sensitivity to pathogens. Reference: O’Rourke et al. (2005) PMID: 16809667. Mutagen: UV/TMP Outcrossed: x3 Made by: J Hodgkin Received: 09/15/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6737 Species: C. elegans Genotype: clec-69&clec-70(ok2061) IV. Description: No obvious phenotype, apparently wild-type sensitivity to pathogens. Derivative of RB1663, further out-crossed into wild-type background. Reference: O’Rourke et al. (2005) PMID: 16809667. Mutagen: UV/TMP Outcrossed: x3 Made by: J Hodgkin Received: 09/15/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6738 Species: C. elegans Genotype: lys-7(ok1384) V. Description: Gross phenotype wildtype, increased sensitivity to some bacterial pathogens. Derivative of RB1285, further out-crossed into wild-type background. References: O’Rourke et al. (2005) PMID: 16809667. Gravato-Nobre et al. (2016) PMID: 27525822. Mutagen: UV/TMP Outcrossed: x3 Made by: J Hodgkin Received: 09/15/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6740 Species: C. elegans Genotype: bus-20(e2990) III; him-5(e1490) V. Description: Anatomically normal hermaphrodites and males; Bus (resistant to infection by M. nematophilum); weakly resistant to Leucobacter Verde2; insensitive to Leucobacter Verde1. Reference: J Hodgkin (unpublished). Mutagen: EMS Outcrossed: x3 Made by: J Hodgkin Received: 09/15/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6742 Species: C. elegans Genotype: dhs-5(e2992) II; him-5(e1490) V. Description: Bus (resistant to M. nematophilum infection); sensitive to Leucobacter Verde2, hypersensitive to Leucobacter Verde1. Reference: M J Gravato-Nobre, J Hodgkin (unpublished). Mutagen: EMS Outcrossed: x3 Made by: J Hodgkin Received: 09/15/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6746 Species: C. elegans Genotype: mif-1(ok2009) III. Description: No obvious phenotype, normal sensitivity to bacterial pathogens. Derived from RB1633; further out-crossed into wild-type background. Reference: O’Rourke et al. (2005) PMID: 16809667. Mutagen: UV/TMP Outcrossed: x3 Made by: J Hodgkin Received: 09/15/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6747 Species: C. elegans Genotype: glf-1(tm2412) IV; nbEx146. Description: nbEx146 [glf-1p::glf-1(+) + sur-5p::GFP]. Pick GFP+ animals to maintain. Array rescues glf-1(null) mutant. Reference: Novelli et al. (2009) PMID: 19751718. Mutagen: UV/TMP Outcrossed: x2 Made by: J Novelli Received: 09/15/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB675 Species: Caenorhabditis elegans Genotype: unc-54(e675) I. Description: Slow moving Unc. Semi-dominant. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6755 Species: C. elegans Genotype: osr-1(ok959) I; glf-1(tm2412) IV. Description: Maintain at 15C. Small, sluggish, slow-growing fragile hermaphrodites. Almost inviable above 20C. Reference: Novelli et al. (2009) PMID: 19751718. Mutagen: Outcrossed: x2 Made by: J Novelli Received: 10/04/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6761 Species: C. elegans Genotype: unc-119(ed3); eEx665. Description: eEx665 [trf-1p::dsRed2 + unc-119(+)]. Pick wild-type to maintain. Transcriptional reporter for trf-1. Reference: Wang et al. (2015) PMID: 26687621. Mutagen: Outcrossed: x2 Made by: M J Gravato-Nobre Received: 09/15/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB677 Species: Caenorhabditis elegans Genotype: unc-60(e677) V. Description: Unc-slow moving. Body muscle abnormal. Semi-dominant. Birefringent patches. Eggs laid. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x1 Made by: Brenner S Received: 07/01/85 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6772 Species: C. elegans Genotype: clec-50(ok2455) V. Description: No obvious phenotype, apparently normal sensitivity to bacterial pathogens. Derived from RB1897; further out-crossed into wild-type background. References: O’Rourke et al. (2005) PMID: 16809667. D J O'Rourke and J Hodgkin, unpublished. Mutagen: UV/TMP Outcrossed: x3 Made by: J Hodgkin Received: 09/15/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB678 Species: Caenorhabditis elegans Genotype: lon-2(e678) X. Description: Long. Mapping marker standard. M-MATING++++ >30%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6785 Species: C. elegans Genotype: unc-119(ed3) III; eEx650. Description: eEx650 [ilys-4p::GFP + unc-119(+)]. Transcriptional reporter for ilys-4. Reference: Gravato-Nobre et al. (2016) PMID: 27525822. Mutagen: Outcrossed: x2 Made by: M J Gravato-Nobre Received: 10/04/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6786 Species: C. elegans Genotype: unc-119(ed3) III; eEx671. Description: eEx671 [ilys-5p::GFP; unc-119(+)]. Pick wild-type (non-Unc) to maintain. Transcriptional reporter for ilys-5. Reference: Gravato-Nobre et al. (2016) PMID: 27525822. Mutagen: Outcrossed: x2 Made by: M J Gravato-Nobre Received: 10/03/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6794 Species: C. elegans Genotype: dhs-5(e2997) II. Description: Bus (resistant to infection by M. nematophilum), sensitive to both Leucobacter Verde1 and Leucobacter Verde2. Reference: O'Rourke et al (in preparation). Mutagen: EMS Outcrossed: x3 Made by: J Hodgkin Received: 10/03/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6858 Species: C. elegans Genotype: srf-10(yj10) bus-12(e2997) IV. Description: Viable, surface abnormal, Bus (resistant to infection by M. nematophilum), resistant to Leucobacter Verde2, hypersensitive to Leucobacter Verde1. Reference: Gravato-Nobre et al. (2011) PMID: 20980242. Mutagen: Outcrossed: x2 Made by: J Hodgkin Received: 10/03/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6881 Species: C. elegans Genotype: bus-22(e2798) III. Description: Bus (resistant to infection by M. nematophilum), resistant to Leucobacter Verde2, hypersensitive to Leucobacter Verde1. Reference: O'Rourke et al (in preparation). Mutagen: EMS Outcrossed: x3 Made by: J Hodgkin Received: 10/03/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6921 Species: C. elegans Genotype: bus-10 & ZK596.4 & ZK596.5 & ZK596.1(e2737) IV. Description: Viable, Bus (M. nematophilum resistant), resistant to Leucobacter Verde2, hypersensitive to Leucobacter Verde1. e2737 is a ~4.5 kb deficiency which removes all of the bus-10 exons, internal ncRNA genes ZK596.4 & ZK596.5, and ZK596.1. Reference: O'Rourke et al (in preparation). Mutagen: mut-7 Outcrossed: x3 Made by: J Hodgkin Received: 10/10/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6931 Species: C. elegans Genotype: bus-10 & ZK596.4 & ZK596.5(e2715) IV; dhs-29(e3014) X. Description: Bus, bleach-sensitive, resistant to Leucobacter Verde2 and Leucobacter Verde1. e2715 is a small deficiency (3191 bp) which removes all of the bus-10 exons and internal ncRNA genes ZK596.4 & ZK596.5. Reference: O'Rourke et al (in preparation). Mutagen: EMS Outcrossed: x2 Made by: J Hodgkin Received: 10/10/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6965 Species: C. elegans Genotype: vab-18(e1210) X. Description: Most L1 larvae are deformed with irregular shapes. Older larvae and adults exhibit variably bent-head. Resembles spon-1(e2623) II. Homozygous viable, but inviable as hemizygotes (e1210/maDf2). Reference: Hodgkin (unpublished). Mutagen: EMS Outcrossed: x4 Made by: J Hodgkin Received: 10/03/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB697 Species: Caenorhabditis elegans Genotype: vab-6(e697) III. Description: Notched head. Roller. Variably DpyRoller. Tail abnormalities. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB6978 Species: C. elegans Genotype: bus-24(e3020) IV. Description: Skiddy, bleach-sensitive, Bus (resistant to infection by M. nematophilum), resistant to Leucobacter Verde2 and to Leucobacter Verde1. Missense mutation in Y11D7A.9. Reference: O'Rourke et al (in preparation). Mutagen: EMS Outcrossed: x2 Made by: J Hodgkin Received: 10/03/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB698 Species: Caenorhabditis elegans Genotype: vab-10(e698) I. Description: Head abnormal-bent. Variable penetrance. Other abnormalities. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 02/01/82 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6990 Species: C. elegans Genotype: srf-2(yj262) bah-1(ok2197) I. Description: Viable, but surface abnormal, Bus (resistant to infection by M. nematophilum), Bah (resistant to Yersinia biofilm). Reference: O'Rourke et al. (in preparation) Mutagen: Outcrossed: x2 Made by: J Hodgkin Received: 10/03/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6991 Species: C. elegans Genotype: mrp-1(pk89) bus-5(br19) X. Description: Skiddy hermaphrodites, bleach-sensitive, drug-hypersensitive, abnormal bacterial pathogen resistance. Reference: Stroud et al (in preparation). Mutagen: Outcrossed: x2 Made by: J Hodgkin Received: 10/04/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB6992 Species: C. elegans Genotype: bus-5(br19) pgp-3(pk18) X. Description: Hypersensitive to drugs. Resistant to infection by M. nematophilum and Leucobacter Verde2. Hypersensitive to Leucobacter Verde1. Reference: O'Rourke et al. (in preparation). Mutagen: Outcrossed: x2 Made by: J Hodgkin Received: 10/03/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7007 Species: C. elegans Genotype: ilys-3(ok3222) IV. Description: Viable, but defective in bacterial grinding and hypersensitive to bacterial pathogens. Derived by out-crossing parental strain VC2496. Reference: Gravato-Nobre et al. (2016) PMID: 27525822. Mutagen: UV/TMP Outcrossed: x3 Made by: M J Gravato-Nobre Received: 10/03/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7080 Species: C. elegans Genotype: eEx754. Description: eEx754 [ilys-3p::ilys-3::mCherry::unc-54 3'UTR + sur-5p::GFP]. Pick GFP+ to maintain. Translational reporter for ilys-3. Reference: Gravato-Nobre et al. (2016) PMID: 27525822. Mutagen: Outcrossed: x2 Made by: M J Gravato-Nobre Received: 10/03/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7101 Species: C. elegans Genotype: dhs-29(e3014) X. Description: Skiddy, bleach-sensitive hermaphrodites. Resistant to infection by Leucobacter Verde1. Reference: O'Rourke et al. (in preparation). Mutagen: EMS Outcrossed: x4 Made by: J Hodgkin Received: 10/03/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7109 Species: C. elegans Genotype: acs-20(e3031) IV; him-5(e1490) V. Description: Him. Skiddy, bleach-sensitive hermaphrodites and males. Resistant to Leucobacter Verde1. Reference: O'Rourke et al. (in preparation). Mutagen: EMS Outcrossed: x3 Made by: J Hodgkin Received: 10/03/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB713 Species: Caenorhabditis elegans Genotype: unc-108(e713) I. Description: Unc. M-MATING++ 1-10%WT. Previously called unc-67. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 09/01/77 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7148 Species: C. elegans Genotype: him-5(e1490) V; bus-8B(tm1410) X; eEx763. Description: eEx763 [bus-8(exonU2stop) + sur-5p::GFP]. Pick GFP+ to maintain. Lethal bus-8 mutation rescued by bus-8 transgene defective in 5' exons U1 and U2. Reference: Stroud et al (in preparation). Mutagen: Outcrossed: x2 Made by: J Hodgkin Received: 10/04/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7151 Species: C. elegans Genotype: him-8(e1489) IV; ptr-15(e2710) V. Description: Him. Bus (resistant to M. nematophilum); hypersensitive to Leucobacter Verde1. References: Gravato-Nobre et al. (2005) PMID: 16079230. Hodgkin et al (in preparation). Mutagen: Outcrossed: x3 Made by: J Hodgkin Received: 10/04/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7171 Species: C. elegans Genotype: tra-1(e1099) subs-4(e3026) III; eDp6 (III;f). Description: Pick wild-type to maintain. Wild-type hermaphrodites segregate wild-type and dead masculinized embryos. e3026 is nonsense mutation in essential gene Y47D3B.1. Reference: O'Rourke et al (in preparation). Mutagen: Outcrossed: x2 Made by: J Hodgkin Received: 10/03/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7172 Species: C. elegans Genotype: ilys-3(ok3222) IV; lys-7(oj1385) V. Description: Viable, poor bacterial grinding, increased sensitivity to bacterial pathogens. Reference: Gravato-Nobre et al. (2016) PMID: 27525822. Mutagen: Outcrossed: x2 Made by: M J Gravato-Nobre Received: 10/03/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7173 Species: C. elegans Genotype: ilys-3(ok3222) IV; ilys-5(tm3151) X. Description: Viable, but with poor bacterial grinding and increased susceptibility to some bacterial pathogens. Reference: Gravato-Nobre et al. (2016) PMID: 27525822. Mutagen: Outcrossed: x2 Made by: M J Gravato-Nobre Received: 09/29/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7174 Species: C. elegans Genotype: ptr-15(e2710e3062) V. Description: Intragenic missense revertant. Partly resistant to Leucobacter Verde1. Reference: Stroud et al. (2013) IWM2013Abstract. ptr-15 also known as bus-13. Mutagen: EMS Outcrossed: x3 Made by: J Hodgkin Received: 09/29/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7176 Species: C. elegans Genotype: bus-8A(lj22) dhs-29(e3066) X. Description: Skiddy, bleach-sensitive, drug-sensitive. Resistant to infection by Leucobacter Verde1 and Leucobacter Verde2. lj22 is a missense mutation (R32C) in bus-8A and might also affect bus-8B (out-of-frame 5'exon U1). Reference: O'Rourke et al (in preparation). Mutagen: Outcrossed: x2 Made by: J Hodgkin Received: 09/29/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7177 Species: C. elegans Genotype: bus-8A&B(lj22e3067) X. Description: Small, skiddy, slow-growing, bleach-sensitive; resistant to Leucobacter Verde1. Intragenic pseudorevertant of bus-8(lj22), P63L = KLHPGDWW> KLHLGDWW. lj22 is a missense mutation (R32C) in bus-8A and might also affect bus-8B (out-of-frame 5'exon U1). Reference: O'Rourke et al (in preparation). Mutagen: EMS Outcrossed: x3 Made by: J Hodgkin Received: 10/04/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7181 Species: C. elegans Genotype: bus-8B(e2883e3071) X. Description: Weak Bus, viable on Leucobacter Verde1. e3071 is a missense intragenic revertant (P97S) of e2883 (G378E). Reference: Stroud et al (in preparation). Mutagen: EMS Outcrossed: x3 Made by: J Hodgkin Received: 09/29/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB719 Species: Caenorhabditis elegans Genotype: unc-1(e719) X. Description: Unc: recessive kinker. Mutagen: EMS Outcrossed: x1 Made by: S. Brenner Received: 03/01/86 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7191 Species: C. elegans Genotype: srf-5(gk424525) X. Description: Bus (M. nematophilum resistant). Weakly resistant to Leucobacter Verde2; hypersensitive to Leucobacter Verde1. Weak missense allele (C59Y). Reference: O'Rourke et al (in preparation). Mutagen: EMS/ENU Outcrossed: x6 Made by: J Hodgkin Received: 09/29/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7198 Species: C. elegans Genotype: acs-20(e3031) IV. Description: Bleach-sensitive, drug-sensitive, resistant to Leucobacter Verde1. Reference: O'Rourke et al (in preparation). Mutagen: EMS Outcrossed: x3 Made by: J Hodgkin Received: 09/29/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7201 Species: C. elegans Genotype: bah-2(gk487599) IV. Description: Bah (resistant to bacterial Biofilm formation by Yersinia). Reference: O'Rourke et al (in preparation). Mutagen: EMS/ENU Outcrossed: x12 Made by: J Hodgkin Received: 10/10/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7212 Species: C. elegans Genotype: unc-119(ed3) III; eEx781. Description: eEx781 [ilys-6p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Transcriptional reporter for ilys-6. Reference: Gravato-Nobre et al. (2016) PMID: 27525822. Mutagen: Outcrossed: x2 Made by: M J Gravato-Nobre Received: 09/29/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB723 Species: Caenorhabditis elegans Genotype: unc-60(e723) V. Description: Semi-paralyzed Unc. Stiff. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 10/01/80 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7248 Species: C.elegans Genotype: dpy-18(e499)/subs-4(e3026) III; wIs78 IV. Description: wIs78 [SCMp::GFP + ajm-1p::GFP + F58E10 (cosmid) + unc-119(+)] IV. Heterozygous strain. Wild-type hermaphrodites segregating wild-type, Dpy-18, and dead eggs (subs-4 homozygotes). Pick wild-type to maintain. Reference: Gravato-Nobre et al (in preparation). Mutagen: Outcrossed: x2 Made by: J Hodgkin Received: 09/29/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7259 Species: C. elegans Genotype: bus-8B(ok1175) X; eEx763. Description: eEx763 [bus-8(exon2stop) + sur-5p::GFP]. Pick GFP+ to maintain. bus-8(ok1175) rescued by modified bus-8(+) transgene. Reference: O'Rourke et al (in preparation). Mutagen: Outcrossed: x2 Made by: J Hodgkin Received: 09/29/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7272 Species: Caenorhabditis elegans Genotype: ccIs4251 I; mIs12 II; dpy-17(e164) III; frIs7 IV; uIs69 V. Description: ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. mIs12 [myo-2p::GFP + pes-10p::GFP + F22B7.9p::GFP] II. frIs7 [nlp-29p::GFP + col-12p::DsRed] IV. uIs69 [pCFJ90(myo-2p::mCherry) + unc-119p::sid-1] V. Mapping strain. This strain is homozygous for integrated fluorescence markers on LG I, II, IV and V, all of which are easily and independently scored using a fluorescent dissecting microscope, plus an easily scored visible marker (dpy-17) for LGIII. The good markers on all five autosomes facilitate linkage assignment of unmapped mutations, and enable rapid replacement of chromosomes when outcrossing heavily mutagenized strains such as those from the Million Mutation Project. Mutagen: Outcrossed: x4 Made by: Jonathan Hodgkin Received: 03/25/14 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB73 Species: Caenorhabditis elegans Genotype: unc-15(e73) I. Description: Paralyzed Unc. Semi-dominant. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7401 Species: C. elegans Genotype: unc-119(ed3) III; bah-2(gk487599) IV; eEx835. Description: eEx835 [bah-2(+) + unc-119(+)]. Pick wild-type to maintain. bah-2(gk487599) rescued by transgene. Reference: O'Rourke et al (in preparation). Mutagen: Outcrossed: x2 Made by: J Hodgkin Received: 09/29/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7403 Species: C. elegans Genotype: bah-3(br9) I; bah-2(gk487599) IV. Description: Bah (resistant to Yersinia biofilm formation). Reference: Hodgkin et al (in preparation). Mutagen: Outcrossed: x2 Made by: J Hodgkin Received: 10/04/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7410 Species: C. elegans Genotype: bah-1(ok2197) I; bah-2(gk487599) IV. Description: Bah (resistant to Yersinia biofilm formation). Reference: Hodgkin et al (in preparation). Mutagen: Outcrossed: x2 Made by: J Hodgkin Received: 10/04/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7422 Species: C. elegans Genotype: bus-22(e3108) subs-4(e3048) III. Description: Viable, small, slow-growing, bleach-sensitive, resistant to Leucobacter Verde1 and Leucobacter Verde2. Lethality of subs-4(e3048) suppressed by bus-22(e3108) mutation. Reference: O'Rourke et al (in preparation). Mutagen: Outcrossed: x2 Made by: J Hodgkin Received: 09/29/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7425 Species: C. elegans Genotype: him-5(e1490) V; bus-25(e3094) X. Description: Him. Small, slow-growing, bleach-sensitive; Bus (resistant to M. nematophilum), slightly resistant to Leucobacter Verde2, hypersensitive to Leucobacter Verde1. Reference: Hodgkin et al (in preparation). Mutagen: EMS Outcrossed: x3 Made by: J Hodgkin Received: 10/04/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7427 Species: C. elegans Genotype: unc-17(e245) IV; eEx849. Description: eEx849 [C28H8.4(sdmV186E)]. unc-17 missense mutant suppressed by missense C28H8.4 transgene. Pick non-Unc hermaphrodites to maintain. Animals that have lost the array are severely Unc (coilers). Reference: Stroud et al (in preparation). Mutagen: Outcrossed: x2 Made by: J Hodgkin Received: 09/29/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7430 Species: C. elegans Genotype: unc-17(e245) IV; eEx855. Description: eEx855 [erd-2(sdmV186E) + sur-5p::GFP]. unc-17 missense mutant partly suppressed by missense erd-2 (a.k.a. sup-2) transgene. Pick GFP+ (weak Unc) hermaphrodites to maintain. Animals that have lost the array are severely Unc (coilers). Reference: Stroud et al (in preparation). Mutagen: Outcrossed: x2 Made by: J Hodgkin Received: 09/29/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7431 Species: Caenorhabditis elegans Genotype: bus-17(br2) X. Description: Altered surface properties; somewhat skiddy movement; drug-sensitive, bleach-sensitive. Resistant to some bacterial pathogens (hence Bus, Bah phenotypes) and hypersensitive to others. Reference: Yook K, Hodgkin J. Genetics. 2007 Feb;175(2):681-97. Mutagen: EMS Outcrossed: x4 Made by: J. Hodgkin Received: 07/20/16 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7448 Species: C. elegans Genotype: bus-5(e3133) X. Description: Slightly skiddy, bleach-sensitive, drug-sensitive, Bus (M. nematophilum resistant), Bah (Yersinia biofilm resistant), Leucobacter Verde1 hypersensitive. CRISPR/Cas9-induced deletion. Reference: O'Rourke et al (in preparation). Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: J Hodgkin Received: 09/29/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7468 Species: C. elegans Genotype: acs-22(gk373989) X. Description: No obvious phenotype; derived from VC20689. Reference: O'Rourke et al (in preparation). Mutagen: EMS/ENU Outcrossed: x4 Made by: J Hodgkin Received: 10/04/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7471 Species: C. elegans Genotype: unc-119(ed3) III; bus-8A(lj22) X; eEx861. Description: eEx861 [bus-8(U1,2)::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. bus-8 exon U1 mutation rescued by small U1,2 transgene. lj22 is a missense mutation (R32C) in bus-8A and might also affect bus-8B (out-of-frame 5'exon U1). Reference: O'Rourke et al (in preparation). Mutagen: Outcrossed: x2 Made by: J Hodgkin Received: 10/10/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7476 Species: C. elegans Genotype: him-8(e1489) IV; galt-1(op497) V. Description: Him. Resistant to fungal toxin CGL2; weakly resistant to Leucobacter Verde1. Him strain derived from pmk-1;galt-1 double mutant. References: Butschi et al (2010). O'Rourke et al (in preparation). Mutagen: Outcrossed: x2 Made by: J Hodgkin Received: 10/10/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7492 Species: C. elegans Genotype: bus-28(gk236264) V. Description: Bus (M. nematophilum resistant), weakly resistant to Leucobacter Verde2, hypersensitive to Leucobacter Verde1. Reference: O'Rourke et al (in preparation). Mutagen: EMS/ENU Outcrossed: x6 Made by: J Hodgkin Received: 10/10/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB75 Species: Caenorhabditis elegans Genotype: mec-2(e75) X. Description: Lethargic. Neurons touch abnormal. Mechanosensory abnormal-touch insensitive. Recessive. Phenotype severe. M-MATING+++ 10-30%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB7505 Species: C. elegans Genotype: him-8(e1489) IV; ptr-15(cr52) V; eEx730. Description: eEx730 [ptr-15(+) + sur-5p::GFP]. Pick GFP+ to maintain. Him. Lethal 1118bp deletion allele of ptr-15 rescued by eEx730. Non-GFP animals will be dead eggs and dead hatchlings. References: O’Rourke et al. (in revision 2024), Kuwabara et al. (in prep.) Mutagen: CRISPR-Cas9 Outcrossed: x3 Made by: J. Hodgkin Received: 11/05/24 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7516 Species: C. elegans Genotype: bus-10(e2702) IV; bus-28(gk236264) V. Description: Viable, Bus (M. nematophilum resistant), resistant to Leucobacter Verde2, hypersensitive to Leucobacter Verde1. Reference: O'Rourke et al (in preparation). Mutagen: Outcrossed: x2 Made by: J Hodgkin Received: 10/10/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7517 Species: C. elegans Genotype: ptr-15(cr52) lon-3(e2175) V; eEx730. Description: eEx730 [ptr-15(+) + sur-5p::GFP]. Pick GFP+ to maintain. Lon. Non-GFP animals will mostly be dead eggs and dead hatchlings, but can segregate rare GFP-negative viable ptr-15 lon-3 homozygotes (which are fertile but grow very poorly). Reference: Kuwabara et al. (in prep.) Mutagen: Outcrossed: x2 Made by: J.Hodgkin Received: 11/05/24 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7537 Species: C. elegans Genotype: srf-2(e3137) I. Description: Srf, Bus, Bah. Amber nonsense mutation: W125* (TIPFFCKW>TIPFFCK*). Reference: O'Rourke et al (in preparation). Mutagen: EMS Outcrossed: x1 Made by: J Hodgkin Received: 10/10/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7538 Species: C. elegans Genotype: lon-2(e678) bus-8B(ok1176) X. Description: Very slow-growing, grows best at 25C. Small, bleach-sensitive, Bus, resistant to Leucobacter Verde1. Reference: O'Rourke et al (in preparation). Mutagen: Outcrossed: x4 Made by: J Hodgkin Received: 10/10/17 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB754 Species: Caenorhabditis elegans Genotype: rol-3(e754) V. Description: Left hand Roller. Incomplete penetrance. M-MATING+POOR <1%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7549 Species: C.elegans Genotype: bus-4(br4) IV. Description: Q288Stop(UAA). Reference null. Surface abnormal, resistant to M. nematophilum and Leucobacter Verde2, killed by Leucobacter Verde1. References: Darby C, et al. Genetics. 2007 May;176(1):221-30. doi: 10.1534/genetics.106.067496. Epub 2007 Mar 4. PMID: 17339204. O’Rourke D, et al. G3 (Bethesda). 2023 May 2;13(5):jkad056. doi: 10.1093/g3journal/jkad056. PMID: 36911920. Mutagen: ENU Outcrossed: x5 Made by: C.Darby Received: 11/05/24 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7550 Species: C. elegans Genotype: erd-2.1(e997) X. Description: Val186Glu. Null allele. Slightly cold-sensitive; dominant suppressor of unc-17(e245). Lethal with erd-2.2(RNAi). Also known as sup-2(e997). Reference: Mathews EA, et al. Genetics. 2021;218(4):iyab065. doi:10.1093/genetics/iyab0. PMID: 33914877. Mutagen: EMS Outcrossed: x4 Made by: J. Hodgkin Received: 11/05/24 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7575 Species: C. elegans Genotype: dhs-29(e3013) X. Description: W242StopUAG. Reference null. Surface abnormal, bleach and drug sensitive; resistant to Leucobacter Verde1. Mutagen: EMS Outcrossed: x6 Made by: J. Hodgkin Received: 11/05/24 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB7587 Species: C. elegans Genotype: ptr-15(gk5234) V; crEx498. Description: crEx498 [dpy-14p::ptr-15(+) + sur-5p::GFP]. Pick animals with nuclear GFP throughout body to maintain. Lethal ptr-15 deletion allele marked with pharyngeal GFP [loxP ::myo-2p::GFP::unc-54 3’UTR + rps-27p::neoR::unc-54 3’UTR::loxP]; lethality rescued by hypodermal expression of PTR-15 form crEx498 array. Non-nuclear GFP animals (only pharyngeal expression) will be dead eggs and dead hatchlings. Derived from parental strain VC4151. References: O’Rourke et al. (in revision 2024). Mutagen: CRISPR-Cas9 Outcrossed: x3 Made by: J.Hodgkin Received: 11/05/24 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB767 Species: Caenorhabditis elegans Genotype: bli-3(e767) I. Description: Blistered cuticle. Bursae abnormal. Dpy. Males abnormal. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 08/01/77 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB768 Species: Caenorhabditis elegans Genotype: bli-2(e768) II. Description: Blistered cuticle. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Sulston J Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB769 Species: Caenorhabditis elegans Genotype: bli-1(e769) II. Description: Blistered cuticle. Male tale abnormal. M-MATING-NO SUCCESS. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 08/01/77 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB78 Species: Caenorhabditis elegans Genotype: unc-6(e78) X. Description: Unc. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB791 Species: Caenorhabditis elegans Genotype: unc-5(e791) IV. Description: Superficially wild-type. References: Rosu S, et al. Science. 2011 Dec 2;334(6060):1286-9. doi: 10.1126/science.1212424. PMID: 22144627. Toraason E, et al. Curr Biol. 2021 Apr 12;31(7):1508-1514.e5. doi: 10.1016/j.cub.2021.03.008. Epub 2021 Mar 18. PMID: 33740427. Toraason E, et al. Elife. 2024 Aug 8;13:e80687. doi: 10.7554/eLife.80687. PMID: 39115289. Mutagen: Outcrossed: x0 Made by: Hodgkin Lab Received: 02/06/25 University of Oregon, Eugene, OR ----------------------------------------------------------------------------- Strain: CB81 Species: Caenorhabditis elegans Genotype: unc-18(e81) X. Description: Recessive, paralysed, kinky, thin at all stages, able to lay eggs. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB840 Species: Caenorhabditis elegans Genotype: dpy-23(e840) X. Description: Dpyish. Head abnormal. Lethal hemizygous. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 06/01/80 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB843 Species: Caenorhabditis elegans Genotype: unc-54(e843) I. Description: Stiff. Body muscle abnormal. Mutagen: X-ray Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB845 Species: Caenorhabditis elegans Genotype: unc-30(e191) IV. Description: Unc-cannot back up. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB873 Species: Caenorhabditis elegans Genotype: eT1 (III;V). Description: M-MATING+POOR <1%WT. Recessive. Poor movement forward and backward. Unc-36 phenotype. Dead eggs. Mutagen: P32 Outcrossed: x Made by: Babu P Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB879 Species: Caenorhabditis elegans Genotype: him-1(e879) I. Description: Segregates males. Recessive. Non-disjunction X-specific. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB88 Species: Caenorhabditis elegans Genotype: dpy-7(e88) X. Description: Dpy. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB883 Species: Caenorhabditis elegans Genotype: unc-74(e883) I. Description: Unc-kinky and coiler. Levamisole resistant. M-MATING++ 1-10%WT. Segregates steriles. Mutagen: P32 Outcrossed: x Made by: Babu P Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB904 Species: Caenorhabditis elegans Genotype: unc-38(e264) I. Description: Levamisole resistant. Recessive. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB91 Species: Caenorhabditis elegans Genotype: rol-1(e91) II. Description: Adults left-handed rollers. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 07/01/77 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB927 Species: Caenorhabditis elegans Genotype: eDf28 IV. Description: Unc-weak kinker. Fairly active. Healthy. See WBPaper00002474. Previously called unc-24(e927). Mutagen: P32 Outcrossed: x Made by: Babu P Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB928 Species: Caenorhabditis elegans Genotype: unc-31(e928) IV. Description: Unc-very slow and sluggish. Insensitive to prodding. Egl. Constitutive pharyngeal pumping. Long lived. Mutagen: Outcrossed: x Made by: Received: 11/13/89 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB933 Species: Caenorhabditis elegans Genotype: unc-17(e245) IV. Description: M-MATING-NO SUCCESS. UNC-Severe coiler at all stages-small and thin. SCORED EASILY. Suppressed by sup-1, sup-2, and snb-1. Resistant to lannate. See also CGC 1770. Mutagen: EMS Outcrossed: x Made by: S. Brenner Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB934 Species: Caenorhabditis elegans Genotype: sma-1(e934) V. Description: Short, round-headed, especially in early larvae. Adults have slight rolling tendency. Males can mate. Mutagen: EMS Outcrossed: x1 Made by: J. Hodgkin Received: 10/01/85 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB936 Species: Caenorhabditis elegans Genotype: unc-73(e936) I. Description: Severe coiler Unc. Bursae abnormal. Males abnormal. M-MATING-NO SUCCESS. Mutagen: P32 Outcrossed: x Made by: Brenner S Received: 12/01/79 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB937 Species: Caenorhabditis elegans Genotype: bli-4(e937) I. Description: M-MATING+POOR <1%WT. Adult blistered, especially head. Mutagen: P32 Outcrossed: x Made by: Babu P Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB94 Species: Caenorhabditis elegans Genotype: unc-1(e94) X. Description: Kinky Unc. Recessive. M-MATING++ 1-10%WT. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CB950 Species: Caenorhabditis elegans Genotype: unc-75(e950) I. Description: Unc-weak coiler, especially in reverse; moves forward well; sluggish; short. Mutagen: Outcrossed: x Made by: Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: CB96 Species: Caenorhabditis elegans Genotype: vab-2(e96) IV. Description: Notched head. Tail abnormalities. Variable expression. Incomplete penetrance. Especially seen in L1. M-MATING++++ >30%WT. See also WBPaper00003843 and WBPaper00003865. Previously called efn-1(e96). Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 01/01/80 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CB998 Species: Caenorhabditis elegans Genotype: unc-52(e998) II. Description: Severe Unc. Paralyzed adults. Dystrophic body muscle. Mutagen: EMS Outcrossed: x Made by: Brenner S Received: 08/01/82 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: CBX102 Species: Microbacterium nematophilum Genotype: Microbacterium nematophilum Description: Bacteria. Caenorhabditis pathogen. Previously called Aureobacterium nematophilum. Biosafety Level: BSL-1. Mutagen: Outcrossed: x Made by: J. Hodgkin Received: 12/04/00 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: CC1 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Continuously cultured in liquid CeMM for 4 years. Mutagen: Outcrossed: x Made by: Nate Szewczyk Received: 12/09/03 Ohio University, Athens, Ohio ----------------------------------------------------------------------------- Strain: CC2 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: N2 stock which was flown on STS-107, shuttle Columbia. Mutagen: Outcrossed: x Made by: Nate Szewczyk Received: 05/01/03 Ohio University, Athens, Ohio ----------------------------------------------------------------------------- Strain: CC3 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: This strain was grown on CeMM (C. elegans Maintenance Medium) for 3 years (called CC1). It was then flown on STS-107, shuttle Columbia (and now called CC3). Mutagen: Outcrossed: x Made by: Nate Szewczyk Received: 05/01/03 Ohio University, Athens, Ohio ----------------------------------------------------------------------------- Strain: CD184 Species: Caenorhabditis elegans Genotype: ace-3(dc2) unc-52(e444) II. Description: Acetylcholine abnormal. Unc (phenotype exactly like unc-52 except lacking class C acetylcholinesterase). Mutagen: Outcrossed: x Made by: Johnson C Received: 02/01/88 Oklahoma Center for Neuroscience ----------------------------------------------------------------------------- Strain: CD194 Species: Caenorhabditis elegans Genotype: lin-7(e1413) unc-52(e444) II. Description: Unc and Vul. Mutagen: Outcrossed: x Made by: Carl Johnson Received: 11/01/94 Hereditary Disease Foundation, New York, NY ----------------------------------------------------------------------------- Strain: CD196 Species: Caenorhabditis elegans Genotype: ace-3(dc2) unc-52(e444) egl-50(n1086) II. Description: Unc and Egl. Mutagen: Outcrossed: x Made by: Carl Johnson Received: 11/01/94 Hereditary Disease Foundation, New York, NY ----------------------------------------------------------------------------- Strain: CDH1 Species: Caenorhabditis elegans Genotype: pduEx1. Description: pduEx1 [pCE-bJUN-VN173 + pCE-bFOX-VC155 + rol-6(su1006)]. Pick Rollers to maintain. Heat-shock induces protein expression and BiFC complementation consistent with expression of the hsp-16.2 promoter. Mutagen: Outcrossed: x0 Made by: YJS/CDH Received: 08/06/08 Purdue University, West Lafayette, IN ----------------------------------------------------------------------------- Strain: CDH2 Species: Caenorhabditis elegans Genotype: pduEx2. Description: pdeEx2 [pCE-bJUN-VN173 + pCE-bFOSdeltaZIP-VC155 + rol-6(su1006)]. Pick Rollers to maintain. Heat-shock induces protein expression but NO BiFC complementation. Mutagen: Outcrossed: x0 Made by: YJS/CDH Received: 08/06/08 Purdue University, West Lafayette, IN ----------------------------------------------------------------------------- Strain: CE1047 Species: Caenorhabditis elegans Genotype: egl-30(ep271) I. Description: Dominant, gain-of-function mutation in egl-30; missense M244I. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x2 Made by: Lisa Moore Received: 02/18/08 Exelixis Inc., San Francisco, CA ----------------------------------------------------------------------------- Strain: CE1051 Species: Caenorhabditis elegans Genotype: goa-1(ep275) I. Description: Gln to Stop mutation in residue 205. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x1 Made by: Lisa Moore Received: 02/18/08 Exelixis Inc., San Francisco, CA ----------------------------------------------------------------------------- Strain: CE1190 Species: Caenorhabditis elegans Genotype: unc-74(x19) gla-3(ep312) I. Description: Gla. May contain dam-1(op241) in background. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Ross Francis Received: 02/18/08 Exelixis Inc., San Francisco, CA ----------------------------------------------------------------------------- Strain: CE1239 Species: Caenorhabditis elegans Genotype: hop-1(ep369) I; sel-12(ep6) spr-3(ep17) X. Description: ep369 is a weak allele. ep6 is a deletion allele. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Ross Francis Received: 03/28/08 Exelixis Inc., San Francisco, CA ----------------------------------------------------------------------------- Strain: CE1255 Species: Caenorhabditis elegans Genotype: cep-1(ep347) I. Description: Resistant to radiation-induced apoptosis (Dam); non-Gla; very weak Rad; no gross phenotype. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x6 Made by: Mike Costa Received: 02/18/08 Exelixis Inc., San Francisco, CA ----------------------------------------------------------------------------- Strain: CE1258 Species: Caenorhabditis elegans Genotype: eat-16(ep273) I. Description: Missense mutation E158K. Worms are Egl-c, Eat, and loopy-Unc. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Robert Choy Received: 02/18/08 Exelixis Inc., San Francisco, CA ----------------------------------------------------------------------------- Strain: CE1338 Species: Caenorhabditis elegans Genotype: epIs14. Description: epIs14 [sbp-1p::GFP + rol-6(su1006)]. Rollers. sbp-1 previously known as pin-1. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Gamma Rays Outcrossed: x0 Made by: Lisa Kadyk Received: 02/18/08 Exelixis Inc., San Francisco, CA ----------------------------------------------------------------------------- Strain: CE1463 Species: Caenorhabditis elegans Genotype: vps-41(ep402) unc-6(e78)/dpy-8(e130) unc-6(e78) X. Description: Heterozygotes are Unc. Segregates DpyUncs. Segregates Uncs which are recessive Mel. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Mike Costa Received: 02/18/08 Exelixis Inc., San Francisco, CA ----------------------------------------------------------------------------- Strain: CE1494 Species: Caenorhabditis elegans Genotype: +/hT2 [dpy-18(h662)] I; pen-2(ep220)/hT2 [bli-4(e937)] III. Description: Heterozgyotes are WT and segregate WT, Dpys, and Ste or Mel. Pick wild-type individuals and check for correct segregation of progeny to maintain. bli-4 is suppressed by dpy-1 in hT2 homozygotes-only see a very few DpyBli.This strain cannot be distributed to commercial organizations. ep220 is likely Mel. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Garth McGrath Received: 02/18/08 Exelixis Inc., San Francisco, CA ----------------------------------------------------------------------------- Strain: CE1570 Species: Caenorhabditis elegans Genotype: vps-35(ep442) rol-1(e91) II. Description: Weak Egl. Rollers. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Garth McGrath Received: 02/18/08 Exelixis Inc., San Francisco, CA ----------------------------------------------------------------------------- Strain: CE1857 Species: Caenorhabditis elegans Genotype: ect-2(e1778)/unc-4(e120) sqt-1(sc13) II. Description: Heterozygotes are WT and segregate WT, Roller Uncs, and ect-2 homozygotes (sterile Uncs which reach adulthood, sometimes giving polynucleate oocytes). ect-2 pka let-21. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Ross Francis Received: 06/01/04 Exelixis Inc., San Francisco, CA ----------------------------------------------------------------------------- Strain: CE541 Species: Caenorhabditis elegans Genotype: sbp-1(ep79) III. Description: Slow growth, low fat stores. Viable at 15C, not at 25C. Slightly Dpy, reduced brood size, low penetrance. Maintain under normal conditions. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Reference: Liang et al., (2010) PlosOne 5:3 e9869. Mutagen: Tc1 insertion and excision Outcrossed: x10 Made by: Kim Ferguson Received: 07/23/10 Washington State University, Pullman WA ----------------------------------------------------------------------------- Strain: CE548 Species: Caenorhabditis elegans Genotype: sbp-1(ep79) III; epEx141. Description: epEx141 [sbp-1::GFP::SBP-1 + rol-6(su1006)]. ep79 is a strong allele of sbp-1 (ts lethal). sbp-1 aka pin-1. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: M. Costa Received: 03/28/08 Exelixis Inc., San Francisco, CA ----------------------------------------------------------------------------- Strain: CE778 Species: Caenorhabditis elegans Genotype: unc-29(e1072) aph-1(ep140)/fog-3(q443) I. Description: This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Ross Francis Received: 02/18/08 Exelixis Inc., San Francisco, CA ----------------------------------------------------------------------------- Strain: CE833 Species: Caenorhabditis elegans Genotype: sbp-1(ep176) III. Description: Weak sbp-1 allele. sbp-1 aka pin-1. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. NOTE: This strain carries an unknown GFP marker. Mutagen: Outcrossed: x Made by: M. Costa Received: 03/28/08 Exelixis Inc., San Francisco, CA ----------------------------------------------------------------------------- Strain: CEN2ent1 Species: Enterobacter xiangfangensis Genotype: Enterobacter xiangfangensis Description: Bacteria. CeMbio Collection. Isolate from soil of mesocosm experiment. More information about collection on the project's wiki: http://www.cembio.uni-kiel.de/. 16S rRNA primer: 27F/1492R. 16S rRNA sequence: TTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAGGAAGCAGCTTGCTGCTTTGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCGGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAAGGCGATAAGGTTAATAACCTTGTCGATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTCTGTCAAGTCGGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATTCGAAACTGGCAGGCTAGAGTCTTGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGTGCCCTTGAGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTACTCTTGACATCCAGAGAACTTAGCAGAGATGCTTTGGTGCCTTCGGGAACTCTGAGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTTTGTTGCCAGCGGTTAGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGAGTAGGGCTACACACGTGCTACAATGGCGCATACAAAGAGAAGCGACCTCGCGAGAGCAAGCGGACCTCATAAAGTGCGTCGTAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGTGGATCAGAATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTT Mutagen: Outcrossed: x0 Made by: Wild isolate Received: 07/17/19 University of California, Berkeley, CA ----------------------------------------------------------------------------- Strain: CER123 Species: Caenorhabditis elegans Genotype: ham-3(he159) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I; III). Description: Heterozygotes are WT, GFP+ in the pharynx and segregate WT (GFP+ in the pharynx), dead eggs (homozygotes for hT2) and ham-3(he159) homozygotes, which are Sma, Egl, Adl, Pvl. Maintain by picking GFP+ worms and checking for correct segregation, since the hT2 balancer is lost at low frequencies. he159 allele was isolated by John Satterlee from a deletion library at Sander van den Heuvel's lab. Reference: Ertl I, et al. Genetics. 2016 Mar;202(3):961-75. Mutagen: Outcrossed: x3 Made by: M. Porta de la Riva Received: 03/28/16 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER162 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; cerIs7. Description: cerIs7 [lsm-1::GFP(fosmid) + unc-119(+)]. cerIs7 rescues all the temperature-sensitive phenotypes of lsm-1(tm3585). Reporter contains full-length lsm-1 tagged with GFP. GFP was inserted into a fosmid containing lsm-1. GFP is expressed diffusely in the cytoplasm of somatic cells. Under heat shock conditions, GFP accumulates forming cytoplasmic foci. Reference: Cornes E, et al. RNA. 2015 Sep;21(9):1544-53. Mutagen: Bombardment Outcrossed: x3 Made by: Eric Cornes Received: 03/28/16 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER178 Species: Caenorhabditis elegans Genotype: nfki-1(cer1) X. Description: cer1 is a CRISPR-generated 368 bp deletion removing intron 2 and part of exon 3, creating a premature stop codon. Low penetrance of developmental defects such as abnormal L1 morphology, aberrant gonad migration, and an abnormal number of distal tip cells. Reference: Brena D, et al. Sci Rep. 2020 Sep 30;10(1):16153. Mutagen: Outcrossed: x3 Made by: Julian Ceron Received: 06/07/21 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER187 Species: Caenorhabditis elegans Genotype: nfki-1(cer2) X. Description: cer2 is a CRISPR-generated 438 bp deletion + 50 bp insertion removing intron 2 and part of exon 3, creating a premature stop codon. Low penetrance of developmental defects such as abnormal L1 morphology, aberrant gonad migration, and an abnormal number of distal tip cells. Reference: Brena D, et al. Sci Rep. 2020 Sep 30;10(1):16153. Mutagen: Outcrossed: x3 Made by: Julian Ceron Received: 06/07/21 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER240 Species: Caenorhabditis elegans Genotype: prp-8(cer22[R2303G]) III. Description: Partial loss of function allele. prp-8(cer22[R2303G]) point mutation mimics a mutation identified in a Retinitis Pigmentosa patient. Slow growth (Gro). Reference: Kukhtar D, et al. Hum Mol Genet. 2020 Mar 27;29(5):756-765. doi: 10.1093/hmg/ddz315. PMID: 31919495 Mutagen: Outcrossed: x0 Made by: Dmytro Kukhtar Received: 07/29/22 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER244 Species: Caenorhabditis elegans Genotype: ikb-1(cer9) I. Description: cer9 is a CRISPR-generated 462 bp deletion at the beginning of the ikb-1 coding sequence, including the start codon (no transcript should be synthesized). Low penetrance of developmental defects such as abnormal L1 morphology, aberrant gonad migration, and an abnormal number of distal tip cells. Reference: Brena D, et al. Sci Rep. 2020 Sep 30;10(1):16153. Mutagen: Outcrossed: x4 Made by: Julian Ceron Received: 06/07/21 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER248 Species: Caenorhabditis elegans Genotype: snrp-200(cer24[S1080L]) II. Description: Partial loss of function allele. snrp-200(cer24[S1080L]) point mutation mimics a mutation identified in a Retinitis Pigmentosa patient. Slow growth (Gro). Reference: Kukhtar D, et al. Hum Mol Genet. 2020 Mar 27;29(5):756-765. doi: 10.1093/hmg/ddz315. PMID: 31919495 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Dmytro Kukhtar Received: 07/29/22 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER250 Species: C. elegans Genotype: ubh-4(cer25[F73V]) II. Description: Superficially wild-type. ubh-4(cer25[F73V]) is a missense mutation mimicking a human BAP1 cancer mutation. Reference: Martinez-Fernandez C, et al. Cells. 2023 Mar 18;12(6):929. doi: 10.3390/cells12060929. PMID: 36980270 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Carmen Martínez-Fernández Received: 05/04/23 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER256 Species: Caenorhabditis elegans Genotype: snrp-200(cer23[V676L]) II. Description: Partial loss of function allele. snrp-200(cer23[V676L]) point mutation mimics a mutation identified in a Retinitis Pigmentosa patient. Reduced brood size, slow growth (Gro). Reference: Kukhtar D, et al. Hum Mol Genet. 2020 Mar 27;29(5):756-765. doi: 10.1093/hmg/ddz315. PMID: 31919495 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Dmytro Kukhtar Received: 07/29/22 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER323 Species: C. elegans Genotype: ubh-4(cer27) II. Description: Reduced brood size. Genetic interaction with rpn-9. cer27 is a 1033 bp deletion removing the start codon and nearly all of the ubh-4 coding sequence. Reference: Martinez-Fernandez C, et al. Cells. 2023 Mar 18;12(6):929. doi: 10.3390/cells12060929. PMID: 36980270 Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Carmen Martínez-Fernández Received: 05/04/23 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER344 Species: C. elegans Genotype: ubh-4(cer32[A87D]) II. Description: Superficially wild-type. ubh-4(cer32[A87D]) is a missense mutation mimicking a human BAP1 cancer mutation. Reference: Martinez-Fernandez C, et al. Cells. 2023 Mar 18;12(6):929. doi: 10.3390/cells12060929. PMID: 36980270 Mutagen: CRISPR/Cas-9 Outcrossed: x0 Made by: Carmen Martínez-Fernández Received: 05/04/23 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER348 Species: Caenorhabditis elegans Genotype: trxr-1(cer35[Sec666C]) IV. Description: Missense mutation selenocysteine to cysteine. Resistant to cisplatin exposure. Primers to genotype this missense mutation and other silent mutations: [Common Fw: GGCTTCCACATTCTCACTCC] [RV wildtype: CTTAACCTCAGCAACCAGAA] [RV Sec to Cys: CTTAACCGCAACATCCGCTG] Reference: García-Rodríguez FJ, et al. Dis Model Mech. 2018 Jun 21;11(6). Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Dmytro Kukhtar Received: 11/27/18 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER36 Species: Caenorhabditis elegans Genotype: lsm-1(tm3585) II; lsm-3(tm5166) IV. Description: Maintain at 15C. Temperature sensitive. Reduced brood size, Sma. Reference: Cornes E, et al. RNA. 2015 Sep;21(9):1544-53. Mutagen: Outcrossed: x4 Made by: Eric Cornes Received: 03/28/16 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER374 Species: Caenorhabditis elegans Genotype: trxr-1(cer55[Sec666X]) IV. Description: Missense mutation engineered by CRISPR removes a selenocysteine to place a stop codon. Resistance to cisplatin exposure. Primers to genotype the cer55 and other silent mutations: [Common Fw: #1224 GGCTTCCACATTCTCACTCC] [RV wildtype: #1414 cTTAACCTCAGCAACCAGAA] [RV Sec to STOP: #1421 CTTAACCTTAACATCCGCTG] Reference: García-Rodríguez FJ, et al. Dis Model Mech. 2018 Jun 21;11(6). Mutagen: Outcrossed: x0 Made by: Carmen Martínez Received: 11/27/18 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER4 Species: Caenorhabditis elegans Genotype: rsr-2(tm2625)/mIn1 [dpy-10(e128) mIs14] II. Description: mIs14 [myo-2::GFP]. Heterozygotes are WT and GFP+ with signal in the pharynx. Heterozygote animals segregate heterozygotes (WT GFP+), mIn1 homozygotes (Dpy and brighter GFP+), and rsr-2(tm2625) homozygotes (Lva non-GFP). Pick WT GFP+ animals and check for proper segregation of progeny to maintain. Reference: Fontrodona L, et al. PLoS Genet. 2013 Jun;9(6):e1003543. Mutagen: Outcrossed: x5 Made by: Laura Fontrodona Received: 11/04/13 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER41 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; cerIs2. Description: cerIs2 [lsm-4p::lsm-4::GFP::lsm-4 3'UTR + unc-119(+)]. Reporter contains full-length lsm-4 tagged with GFP. GFP was inserted into a fosmid containing lsm-4. Ubiquitous nuclear GFP expression in somatic and germ cells under normal growth conditions. Cytoplasmic GFP accumulation after exposure to heat-shock. Reference: Cornes E, et al. RNA. 2015 Sep;21(9):1544-53. Mutagen: Bombardment Outcrossed: x0 Made by: Eric Cornes Received: 03/28/16 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER444 Species: Caenorhabditis elegans Genotype: sftb-1(cer114[mCherry::sftb-1]) III. Description: Endogenous sftb-1 reporter generated by CRISPR/Cas9 using the Nested CRISPR protocol (Vicencio et al., 2019 Genetics). mCherry was amplified from pJJR83 plasmid and inserted at the 5' end of the sftb-1 gene. External primers used for genotyping: (For: AGCTATCGAAGTTTAGGATGTTGTT) (Rev: CGGTTCCAATCGAGTCTAGGTA) Reference: Serrat X, et al. PLoS Genet. 2019 Oct 21;15(10):e1008464. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Xènia Serrat Received: 11/12/19 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER461 Species: Caenorhabditis elegans Genotype: nfki-1(cer116[eGFP::nfki-1]) X. Description: eGFP tag inserted into the endogenous nfki-1 locus. eGFP::NFKI-1 signal is observed in diverse neurons in the animals' head and tail throughout post-embryonic development. No obvious phenotype. Reference: Brena D, et al. Sci Rep. 2020 Sep 30;10(1):16153. Mutagen: CRIPSR/Cas9 Outcrossed: x1 Made by: Julian Ceron Received: 06/07/21 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER522 Species: C. elegans Genotype: ubh-4(cer140) rpn-9(gk401)/mIn1 [mIs14 dpy-10(e128)] II. Description: Homozygous viable mutation balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP cer140 gk401 homozygotes (synthetic sterile). Pick WT dim GFP and check for correct segregation of progeny to maintain. Generated by CRISPR-mediated deletion of ubh-4 in gk401 mutant background. Reference: Martinez-Fernandez C, et al. Cells. 2023 Mar 18;12(6):929. doi: 10.3390/cells12060929. PMID: 36980270 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Carmen Martínez-Fernández Received: 05/19/23 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER529 Species: Caenorhabditis elegans Genotype: sftb-1(cer144) III. Description: Dose-dependent sensitivity (developmental arrest) to pladienolide B and herboxidiene (modulators of pre-mRNA splicing). sftb-1(cer144[S1090A, A1095T, I1096V, F1101Y]) contains four missense mutations reproducing the HEAT repeat 15 of the human SF3B1 protein. Ten silent mutations increase primer specificity for PCR genotyping. Primers used for genotyping: (WT For: GAGCTGCAATTAATACATTTGGATTT) (WT Rev: AAACTCGCATTCCTTCACAT) (cer144 For: GGTACTATTCTGTGGCGTCT) (cer144 Rev: GTAACCGAAAGTGTTCACAGTT) Reference: Serrat X, et al. PLoS Genet. 2019 Oct 21;15(10):e1008464. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Xènia Serrat Received: 11/12/19 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER554 Species: Caenorhabditis elegans Genotype: comt-4(cer157[comt-4p::GFP::H2B]) V. Description: No obvious phenotype. comt-4(cer157) is a complete deletion of the comt-4 gene (coding sequence + introns), which was replaced by GFP::H2B (Nested CRISPR, Vicencio et al, Genetics 2019), thereby creating a null allele and a transcriptional reporter at the same time. Reference: Martínez-Fernández C, et al. Dis Model Mech. 2022 Mar 1;15(3):dmm049161. doi: 10.1242/dmm.049161. PMID: 35107130 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Carmen Martínez-Fernández Received: 07/29/22 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER588 Species: Caenorhabditis elegans Genotype: cat-2(cer181[cat-2p::GFP::H2B 1-3]) II. Description: Abnormal locomotion can be rescued with dopamine. cat-2(cer181) is a complete deletion of the cat-2 gene (coding sequence + introns), which was substituted by the sequence of the step 1 repair for GFP::H2B (Nested CRISPR, Vicencio et al, Genetics 2019). Allele can be detected using the following primers: Fwd: ctatgtgaagtcacacctgtc Rev: cttgctggaagtgtacttggtg. Reference: Martínez-Fernández C, et al. Dis Model Mech. 2022 Mar 1;15(3):dmm049161. doi: 10.1242/dmm.049161. PMID: 35107130 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Carmen Martínez-Fernández Received: 07/29/22 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER620 Species: C. elegans Genotype: ubh-4(cer68[ubh-4::eGFP]) rpn-9(cer203[rpn-9::wrmScarlet]) II Description: eGFP tag inserted into endogenous ubh-4 locus. wrmScarlet tag inserted into endogenous rpn-9 locus. Reference: Martinez-Fernandez C, et al. Cells. 2023 Mar 18;12(6):929. doi: 10.3390/cells12060929. PMID: 36980270 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Carmen Martínez-Fernández Received: 05/04/23 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER660 Species: Caenorhabditis elegans Genotype: cer227[mex-5p::SpG(smu-2 introns) + unc-119(+)] II; unc-119(ed3) III. Description: Missense mutations D1135L and S1136W, G1218K and E1219Q, and R1335Q and T1337R were introduced on the Cas9 gene at EG9615 strain, to cause endogenous expression of the Cas9 variant SpG. SpG is efficient for CRISPR on NGN PAM sites. Reference: Vicencio J, et al. Nature Communication, 2022. May 12;13(1):2601. doi: 10.1038/s41467-022-30228-4. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Dmytro Kukhtar Received: 07/29/22 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CER7 Species: Caenorhabditis elegans Genotype: rsr-2(tm2607) II. Description: Superficially wild-type. tm2607 is a 196 bp deletion + 1bp insertion that removes part of an essential gene, but produces viable animals. Reference: Fontrodona L, et al. PLoS Genet. 2013 Jun;9(6):e1003543. Mutagen: Outcrossed: x3 Made by: Laura Fontrodona Received: 11/04/13 Fundacion IDIBELL, Barcelona, Spain ----------------------------------------------------------------------------- Strain: CEW1 Species: Oscheius tipulae Genotype: Oscheius tipulae. Description: Isolated in 1991 by Carlos E. Winter in soil samples taken at the University of Sao Paulo in Brazil. Hermaphrodite strain. Adults are 1.5mm. The life cycle is a little longer than C. elegans at 22C. Each lays about 300 eggs in the three days following the moult from L4 to adult. Eggs are laid just after being fertilized resulting sometimes in plates with many eggs (much more than C. elegans). See Comp. Biochem. Physiol 103B: 189, 1992. See Nematology 2(1): 89-98, 2000. Can be grown and maintained on NGM. L1s easily frozen and stored in liquid nitrogen. This strain is deposited in Paul Sternberg's collection under the name PS1022. The species has not yet been determined; Lynn Carta will publish a paper proposing Oscheius brevesophaga. DO NOT use this name before the paper is published. Contact Carles E. Winter or Lynn Carta before publishing anything official about this strain. See also WBPaper00004471 and WBPaper00004485. AKA Oscheius sp. 1. Mutagen: Outcrossed: x Made by: Carlos E. Winter Received: 09/05/95 University of Sao Paulo, Sao Paulo, Brazil ----------------------------------------------------------------------------- Strain: CF1038 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I. Description: Dauer defective. Short lived. Mutagen: TMP + UV Outcrossed: x11 Made by: C. Kenyon and K. Kin Received: 04/04/00 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1045 Species: Caenorhabditis elegans Genotype: muIs49. Description: muIs49 [unc-22(+) egl-20::GFP]. Rescuing translational egl-20::GFP fusion. Not known to which LG muIs49 is attached. A few cells in the tail will light up with GFP. Mutagen: Gamma Rays Outcrossed: x3 Made by: J. Whangbo Received: 03/29/02 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1097 Species: Caenorhabditis elegans Genotype: ref-1(mu220) II. Description: P9.p and P10.p fail to fuse with hyp7 during L1 in hermaphrodites. Misshapen head (low penetrance) most visible in L1. Ectopic postdeirid generated by V6 (low penetrance). Mutagen: EMS Outcrossed: x3 Made by: Scott Alper Received: 05/14/01 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1135 Species: Caenorhabditis elegans Genotype: egl-20(n585) IV; muEx68. Description: muEx68 [(pJW33) myo-2p::egl-20::GFP + (7PD10.46) unc-22 (antisense)]. Use nicotine or levamisole to pick twitchers. Reference: Whangbo J & Kenyon C, (1999) Mol Cell 4(5):851-8. Mutagen: Outcrossed: x Made by: Jennifer Whangbo Received: 08/23/10 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1137 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; daf-2(e1370) III; muIs61. Description: muIs61 [daf-16a::GFP + rol-6(su1006)]. Temperature-sensitive. Maintain at 15 C. Rollers. Reference: Lin K, Hsin H, Libina N, Kenyon C. Nat Genet. 2001 Jun;28(2):139-45. Mutagen: Outcrossed: x2 Made by: Kui Lin Received: 06/13/11 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1139 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; muIs61. Description: muIs61 [(pKL78) daf16::GFP + rol-6(su1006)]. muIs61 rescues daf-16(mu86). muIs61 is a gamma-induced insertion of muEx50. muIs61 insertion point has not been mapped. Mutagen: Gamma-induced insertion Outcrossed: x1 Made by: K. Lin Received: 07/23/01 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1170 Species: Caenorhabditis elegans Genotype: egl-20(n585) IV; muEx79. Description: muEx79 [(pJW33) myo-2p::egl-20::GFP + (7PD10.46) unc-22 (antisense)]. Use nicotine or levamisole to pick twitchers. Reference: Whangbo J & Kenyon C, (1999) Mol Cell 4(5):851-8. Mutagen: Outcrossed: x Made by: Jennifer Whangbo Received: 08/23/10 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1192 Species: Caenorhabditis elegans Genotype: egl-27(n170) II; muIs35 V. Description: muIs35 [mec-7::GFP + lin-15(+)]. Mutagen: Outcrossed: x Made by: Queelim Ch'ng Received: 05/03/05 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF12 Species: C. elegans Genotype: rol-6(e187) II; lin-22(n372) IV; him-5(e1490) V. Description: Rollers. lin-22 and him-5 mutations affect neuroblast formation from epidermal precursor cell V5. Mutagen: N/A Outcrossed: x0 Made by: David A. Waring Received: 06/21/17 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1259 Species: Caenorhabditis elegans Genotype: mig-13(mu225) lin-15B&lin-15A(n765) X; muIs62. Description: muIs62 [mig-13p::mig-13::GFP + lin-15(+)]. Mutagen: Outcrossed: x0 Made by: Queelim Ch'ng Received: 05/03/05 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1295 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; daf-2(e1370) III; muEx108. Description: muEx108 [(pKL99-2) daf-16::GFP/daf16bKO + rol-6(su1006)]. Grows okay at 20C. Rollers should form dauers at 25C. Pick Rollers to maintain. Mutagen: Outcrossed: x Made by: Kui Lin Received: 10/23/01 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1308 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; muEx116. Description: muEx116 [daf-16a::GFP(T54A S240A T242A S314A) + rol-6(su1006)]. Grows at all temperatures. Carries extrachromosomal array. Pick Rollers to maintain. Mutagen: Outcrossed: x Made by: Kui Lin Received: 10/23/01 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1330 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; muEx128. Description: muEx128 [(pKL79) daf-16a::GFP) + rol-6(su1006)]. Grows okay at 20C. Pick Rollers to maintain. Mutagen: Outcrossed: x Made by: Kui Lin Received: 10/23/01 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1371 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; muEx151. Description: muEx151 [(pKL106-8) daf-16aAM::GFP/bKO + rol-6(su1006)]. Grows okay at 20C. Pick Rollers to maintain. Mutagen: Outcrossed: x Made by: Kui Lin Received: 10/23/01 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1380 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; daf-2(e1370) III; muEx158. Description: muEx158 [daf-16cAM::GFP + sur-5p::GFP] (AM = AKT-site mutant). Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less. muEx158 contains GFP-tagged daf-16 c isoform (described as a1 isoform in Lin, et al. Nat Genet. 2001) with 4 Ser/Thr residues mutated to Ala, which completely rescues dauer formation and partially restores longevity of daf-16; daf-2 double mutants. Reference: Lin K, et al. Nat Genet. 2001 Jun;28(2):139-45. Mutagen: Outcrossed: x Made by: Kui Lin Received: 12/06/16 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1395 Species: Caenorhabditis elegans Genotype: ceh-20(mu290) III; muIs164. Description: muIs164 [tax-4::GFP]. Mutagen: EMS (mu290) Outcrossed: x3 Made by: Lucie Yang Received: 03/27/07 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1407 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; muIs71 X. Description: muIs71 [(pKL99) daf-16ap::GFP::daf-16a(bKO)) + rol-6(su1006)]. Grows okay at 20C. Spontaneous integrant. Rollers. Mutagen: Outcrossed: x Made by: Kui Lin Received: 10/23/01 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1442 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; daf-2(e1370) III; muEx169. Description: muEx169 [unc-119p::GFP::daf-16 + rol-6(su1006)]. Pick Rollers to maintain. May grow better at 15C. Mutagen: Outcrossed: x Made by: Nataliya Libina Received: 01/19/05 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1449 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; daf-2(e1370) III; muEx176. Description: muEx176 [daf-16p::GFP::daf-16 + rol-6(su1006)]. Pick rollers to maintain -- Low transmission rate! Maintain at 15C. Forms dauers at 25C. Reference: Lin K, et al. Nat Genet. 2001 Jun;28(2):139-45. Mutagen: Outcrossed: x Made by: N Libina & C Kenyon Received: 01/05/12 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1514 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; daf-2(e1370) III; muEx211. Description: muEx211[pNL213(ges-1p::GFP::daf-16) + rol-6(su1006)]. Grows at 15C (probably also at 20C). Pick Rollers to maintain. Mutagen: Outcrossed: x Made by: Nataliya Libina Received: 01/13/05 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1515 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; daf-2(e1370) III; muEx212. Description: muEx212[pNL212(myo-3p::GFP::daf-16) + rol-6(su1006)]. Grows at 15C (probably also at 20C). Pick Rollers to maintain. Mutagen: Outcrossed: x Made by: Nataliya Libina Received: 01/13/05 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1553 Species: Caenorhabditis elegans Genotype: muIs84. Description: muIs84 [(pAD76) sod-3p::GFP + rol-6(su1006)]. Green expression in head, tail and around vulva. Many animals roll weakly or not at all, but still express GFP. Grows at all temperatures. Mutagen: Outcrossed: x3 Made by: Libina & Berman Received: 01/06/04 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1580 Species: Caenorhabditis elegans Genotype: daf-2(e1370) III; muIs84. Description: muIs84 [(pAD76) sod-3p::GFP + rol-6(su1006)]. Green expression in head, tail and around vulva. Daf-C at 25C. Grows well at 20C. Mutagen: Outcrossed: x Made by: N. Libina Received: 01/21/04 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1588 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; daf-2(e1370) III; muIs84. Description: muIs84 [(pAD76) sod-3p::GFP + rol-6(su1006)]. Dim green expression in head, tail and around vulva. Daf-d. Can grow at 20C. Mutagen: Outcrossed: x Made by: N. Libina Received: 01/21/04 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1595 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; daf-2(e1370) III; muEx227. Description: muEx227 [(pNL213) ges-1p::GFP::daf-16) + rol-6(su1006)]. Pick Rollers to maintain. Mutagen: Outcrossed: x Made by: Nataliya Libina Received: 01/13/05 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF162 Species: Caenorhabditis elegans Genotype: mig-2(mu28) X. Description: mig-2 null mutation. Animals look grossly WT but Q descendants and other migratory neurons misplaced. Mutagen: EMS Outcrossed: x3 Made by: Judith Austin Received: 11/29/99 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1632 Species: Caenorhabditis elegans Genotype: unc-62(mu232) muIs35 V. Description: muIs35 [mec-7::GFP + lin-15(+)]. Egl. GFP+. QR pax migration defect. Mutagen: Gamma-induced insertion Outcrossed: x0 Made by: Lucie Yang Received: 12/18/07 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1641 Species: Caenorhabditis elegans Genotype: qid-6(mu252) III. Description: QL descendants migrate anteriorly. Bli. Unc. Weak Dpy. Mutagen: EMS Outcrossed: x3 Made by: Q Ch'ng/Y Lie Received: 01/28/04 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1660 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; daf-2(e1370) III; muIs84; muEx211. Description: muIs84 [(pAD76) sod-3p::GFP + rol-6(su1006)]. muEx211 [ges-1p::daf-16::GFP + rol-6(su1006)]. Pick Rollers to maintain. Partial rescue of lifespan phenotype. Some animals show variable daf-16 expression in the intestine. Grows okay at 15C. [NOTE: muEx211 is quite unstable. Be sure to pick Rollers to avoid losing the array.] Mutagen: Outcrossed: x Made by: N. Libina Received: 01/22/04 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1665 Species: Caenorhabditis elegans Genotype: muIs32 II; mig-15(mu327) X. Description: muIs32 [mec-7p::GFP + lin-15(+)]. QL descendants migrate anteriorly; defects in HSN migration, male tail formation and PLM axon outgrowth. Egl. Unc. Pvl. Weak Dpy. Mutagen: EMS Outcrossed: x3 Made by: Q Ch'ng/Y Lie Received: 01/28/04 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1667 Species: Caenorhabditis elegans Genotype: mig-15(mu342) X. Description: QL descendants migrate anteriorly; defects in HSN migration, male tail formation and PLM axon outgrowth. Egl. Unc. Pvl. Weak Dpy. Mutagen: EMS Outcrossed: x3 Made by: Q Ch'ng/Y Lie Received: 01/28/04 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1700 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; mes-1(bn7) X; muEx248. Description: muEx248 [(pNL209) daf-16::GFP::daf-16(cDNA) + podr-1::RFP]. Pick green (body) / red (head neurons) animals. Transmission efficiency ~50%. Can be grown at 20C with some sterility (30-50%). The higher the temperture, the greater the sterility. Mutagen: Outcrossed: x Made by: Nataliya Libina Received: 11/28/05 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1724 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; daf-2(e1370) III; muIs105. Description: muIs105 [daf-16p::GFP::daf-16 + rol-6(su1006)]. Rollers; Rol phenotype is not always evident). Integrated line derived from CF1449. Maintain at 15C. Forms dauers at 25C. Reference: Lin K, et al. Nat Genet. 2001 Jun;28(2):139-45. Mutagen: Outcrossed: x Made by: M Hansen & C Kenyon Received: 01/05/12 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1756 Species: Caenorhabditis elegans Genotype: ptp-3(mu245) muIs32 II. Description: muIs32 [mec-7p::GFP + lin-15(+)]. QL descendants migrate anteriorly. Mutagen: EMS Outcrossed: x3 Made by: Q Ch'ng/Y Lie Received: 01/28/04 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1806 Species: Caenorhabditis elegans Genotype: ceh-20(mu290) III; muEx261. Description: muEx261[ceh-20::GFP at C terminus + odr-1::RFP]. Mutagen: Outcrossed: x Made by: Lucie Yang Received: 03/09/07 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1814 Species: Caenorhabditis elegans Genotype: rrf-3(pk1426) II; daf-2(e1370) III. Description: Daf-c at 25.5C; grow at 20C or less. Long lived. Mutagen: Outcrossed: x Made by: C Kenyon/M Hansen Received: 06/05/08 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1824 Species: Caenorhabditis elegans Genotype: muEx265. Description: muEx265 [hsf-1p::hsf-1(cDNA) + myo-3::GFP]. Pick GFP worms to maintain. Mutagen: Outcrossed: x Made by: Ao-lin Hsu Received: 04/21/05 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1827 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; daf-2(e1370) III; muEx268. Description: muEx268 [ges-1p::GFP::daf-16(cDNA) + odr-1::RFP]. daf-16 GFP expressed in intestine. Partial rescue of lifespan phenotype. Grows okay at 15C. Pick RFP to maintain. Mutagen: Outcrossed: x Made by: N. Libina Received: 01/22/04 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1844 Species: Caenorhabditis elegans Genotype: rrf-3(b26) II; daf-2(mu150) III; fem-1(hc17) IV. Description: Long lifespan. Maintain at 15C. Worms develop slowly at 20C, and are sterile at 25C. Reference: Garigan D, et al. Genetics. 2002 Jul;161(3):1101-12. Mutagen: EMS Outcrossed: x3 Made by: Delia Garigan Received: 11/21/13 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1864 Species: Caenorhabditis elegans Genotype: daf-10(mu377) IV. Description: Temperature-sensitive allele of daf-10 exhibits dye filling (Dyf) phenotype at 25C and non-Dyf at 20C. Mutagen: Outcrossed: x2 Made by: Joy Alcedo Received: 12/06/16 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1874 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; muIs84. Description: muIs84 [(pAD76) sod-3p::GFP + rol-6(su1006)]. Green expression in head, tail and around vulva. Mutagen: Gamma Rays Outcrossed: x Made by: Malene Hansen Received: 03/23/07 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1880 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; glp-1(e2141) III. Description: Sterile at 25C; grow at 20C or less. glp-1(e2141) longevity suppressed by daf-16(mu86). Mutagen: Outcrossed: x Made by: C Kenyon/M Hansen Received: 06/05/08 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1903 Species: Caenorhabditis elegans Genotype: glp-1(e2144) III. Description: Temperature sensitive. Sterile at 25C. Maintain at 15C. [NOTE: CF1903 carries glp-1(e2144), not e2141 as previously described.] Mutagen: Outcrossed: x3 Made by: C Kenyon/J Berman Received: 06/05/08 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1935 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; glp-1(e2141) III; muIs109. Description: muIs109 [daf-16p::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. Mutagen: Gamma Rays Outcrossed: x Made by: C Kenyon/M Hansen Received: 06/05/08 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF196 Species: Caenorhabditis elegans Genotype: muIs3 V. Description: muIs3 [mab-5::lacZ + rol-6(su1006)] V. Rollers. Mutagen: array integrated by gamma rays Outcrossed: x3 Made by: Deborah Cowing Received: 11/29/99 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF1980 Species: Caenorhabditis elegans Genotype: rrf-3(pk1426) II; daf-2(e1368) III. Description: Daf-c at 25.5C; grow at 20C or less. Long lived. Mutagen: Outcrossed: x Made by: C Kenyon/J Berman Received: 06/05/08 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2005 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; daf-2(e1370) III; muIs120. Description: muIs120 [ges-1p::GFP::daf-16 + rol-6(su1006)]. Maintain at 15-20C. Rollers. Long-lived. Gamma irradiation-induced integration of muEx211. Rescues daf-16a1/c in the intestine (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100. Mutagen: gamma irradiation Outcrossed: x4 Made by: Peichuan Zhang Received: 07/07/14 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2018 Species: Caenorhabditis elegans Genotype: muEx304. Description: muEx304 [lys-7p::RFP(NLS) + rol-6(su1006)]. Pick Rollers to maintain. Reference: Zhang P, et al. Cell Metab. 2013 Jan 8;17(1):85-100. Mutagen: Outcrossed: x0 Made by: Peichuan Zhang Received: 09/27/16 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2031 Species: Caenorhabditis elegans Genotype: muEx306. Description: muEx306 [tcer-1::GFP + odr-1::RFP]. Maintain by picking RFP+ animals. Reference: Gahzi A, et al. PLoS Genet. 2009 Sep;5(9):e1000639. Mutagen: Outcrossed: x Made by: Arjumand Ghazi Received: 07/12/11 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2037 Species: Caenorhabditis elegans Genotype: muEx311. Description: muEx311 [pep-2p::RFP(NLS) + rol-6(su1006)]. Pick Rollers to maintain. pep-2 is an other name for pept-1. Reference: Zhang P, et al. Cell Metab. 2013 Jan 8;17(1):85-100. Mutagen: Outcrossed: x0 Made by: Peichuan Zhang Received: 09/27/16 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2038 Species: Caenorhabditis elegans Genotype: muEx312. Description: muEx312 [dod-17p::RFP(NLS) + rol-6(su1006)]. Pick Rollers to maintain. Reference: Zhang P, et al. Cell Metab. 2013 Jan 8;17(1):85-100. Mutagen: Outcrossed: x0 Made by: Peichuan Zhang Received: 09/27/16 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2052 Species: Caenorhabditis elegans Genotype: kri-1(ok1251) I. Description: Reference: Berman JR, Kenyon C. Cell. 2006 Mar 10;124(5):1055-68. Mutagen: Outcrossed: x3 Made by: J Berman & C Kenyon Received: 01/05/12 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2060 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; muEx158. Description: muEx158 [daf-16cAM::GFP + sur-5p::GFP] (AM = AKT-site mutant). Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less. muEx158 contains GFP-tagged daf-16 c isoform (described as a1 isoform in Lin, et al. Nat Genet. 2001) with 4 Ser/Thr residues mutated to Ala, which rescues daf-16 mutants from defective dauer formation and also partially rescues longevity defect of daf-16; glp-1 double mutants. Reference: Berman JR, & Kenyon C. Cell. 2006 Mar 10;124(5):1055-68. Mutagen: Outcrossed: x Made by: Jennifer Berman Received: 12/06/16 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2061 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; glp-1(e2141) III; muEx158. Description: muEx158 [daf-16AM::GFP + sur-5p::GFP]. Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less. Mutagen: Outcrossed: x Made by: C Kenyon/J Berman Received: 06/24/08 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2065 Species: Caenorhabditis elegans Genotype: kri-1(ok1251) I; glp-1(e2141) III. Description: Temperature-sensitive. Sterile at 25C. [NOTE: can be grown at 20C, but 15C might be better for long-term propagation.] kri-1(ok1251) suppresses the longevity of glp-1(e2141) mutants. Mutagen: Outcrossed: x3 Made by: C Kenyon/J Berman Received: 06/05/08 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2093 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; daf-2(e1370) III; muIs131. Description: muIs131 [unc-119p::GFP::daf-16 + rol-6(su1006)]. Maintain at 15-20C. Rollers. Modestly long-lived. Gamma irradiation-induced integration of muEx184. Rescues daf-16a1/c in neurons (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100. Mutagen: gamma irradiation Outcrossed: x4 Made by: Peichuan Zhang Received: 07/07/14 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2102 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; daf-2(e1370) III; muIs126. Description: muIs126 [myo-3p::GFP::daf-16 + rol-6(su1006)]. Maintain at 15-20C. Rollers. Gamma irradiation-induced integration of muEx215. Rescues daf-16a1/c in muscles (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100. Mutagen: gamma irradiation Outcrossed: x4 Made by: Peichuan Zhang Received: 07/07/14 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2124 Species: Caenorhabditis elegans Genotype: muIs139. Description: muIs139 [dod-11p::RFP::NLS + rol-6(su1006)]. Pick Rollers to maintain. Reference: Zhang P, et al. Cell Metab. 2013 Jan 8;17(1):85-100. Mutagen: Outcrossed: x4 Made by: P Zhang & L Mitic Received: 09/27/16 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2135 Species: Caenorhabditis elegans Genotype: daf-16(mu86) kri-1(ok1251) I; glp-1(e2141) III; muIs109. Description: muIs109 [daf-16p::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. Mutagen: Outcrossed: x Made by: C Kenyon/J Berman Received: 06/05/08 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2154 Species: Caenorhabditis elegans Genotype: tcer-1(tm1452) II; glp-1(e2141) III. Description: Temperature sensitive sterile at 25C. tcer-1(-) causes loss of germ cells and increases lifespan. Reference: Ghazi A, Henis-Korenbilt S, & Kenyon C (2009) PLoS Genet 5(9):e1000639. Mutagen: Outcrossed: x Made by: Arjumand Ghazi Received: 08/23/10 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2164 Species: C. elegans Genotype: muEx329. Description: muEx329 [jnk-1::GFP + myo-3p::RFP]. Pick RFP+ to maintain. Mutagen: N/A Outcrossed: x0 Made by: Sivan Korenblit Received: 06/21/17 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2167 Species: Caenorhabditis elegans Genotype: tcer-1(tm1452) II. Description: Temperature sensitive sterile at 25C. tcer-1(-) causes loss of germ cells and increases lifespan. Reference: Ghazi A, Henis-Korenbilt S, & Kenyon C (2009) PLoS Genet 5(9):e1000639. Mutagen: Outcrossed: x3 Made by: Arjumand Ghazi Received: 08/23/10 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2218 Species: Caenorhabditis elegans Genotype: ncl-1(e1942) III. Description: Abnormal large nucleoli in most cells. Mutagen: Outcrossed: x4 Made by: D. Frank Received: 05/13/05 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2222 Species: Caenorhabditis elegans Genotype: muEx336. Description: muEx336 [mtl-1::RFP + rol-6(su1006)]. Pick Rollers to maintain. Reference: Zhang P, et al. Cell Metab. 2013 Jan 8;17(1):85-100. Mutagen: Outcrossed: x0 Made by: Peichuan Zhang Received: 05/02/13 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2247 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; glp-1(e2141) III; muEx248. Description: muEx248 [daf-16::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. Pick RFP+/GFP+ animals to maintain. Mutagen: Outcrossed: x Made by: C Kenyon/J Berman Received: 06/24/08 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2248 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; glp-1(e2141) III; daf-12(rh61rh411) X; muEx158. Description: muEx158 [daf-16AM::GFP + sur-5p::GFP]. Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less. Mutagen: Outcrossed: x Made by: C Kenyon/J Berman Received: 06/24/08 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2263 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; glp-1(e2141) III; daf-9(rh50) X; muEx158. Description: muEx158 [daf-16AM::GFP + sur-5p::GFP]. Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less. Mutagen: Outcrossed: x Made by: C Kenyon/J Berman Received: 06/24/08 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2265 Species: Caenorhabditis elegans Genotype: daf-16(mu86) kri-1(ok1251) I; glp-1(e2141) III; muEx158. Description: muEx158 [daf-16AM::GFP + sur-5p::GFP]. Pick GFP+ worms to maintain. Sterile at 25C; grow at 20C or less. Mutagen: Outcrossed: x Made by: C Kenyon/J Berman Received: 06/24/08 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2266 Species: Caenorhabditis elegans Genotype: muEx340. Description: muEx340 [ges-1p::RFP + ges-1p::ins-7]. Intestinal expression of ins-7 shortens lifespan. Pick RFP animals to maintain. Mutagen: Outcrossed: x0 Made by: Seung-Jae Lee Received: 02/07/08 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2278 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; glp-1(e2141) III; daf-12(rh61rh411) X; muEx248. Description: muEx248 [daf-16p::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. Pick RFP+/GFP+ animals to maintain. Mutagen: Outcrossed: x Made by: C Kenyon/J Berman Received: 06/05/08 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2282 Species: Caenorhabditis elegans Genotype: muEx344. Description: muEx344 [kri-1p::GFP::kri-1a cDNA + odr-1::RFP]. Maintain by picking RFP+ animals. Reference: Berman JR and Kenyon C. Cell. 2006 Mar 10;124(5):1055-68. Mutagen: Outcrossed: x Made by: Jennifer Berman Received: 06/13/11 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2288 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; glp-1(e2141) III; daf-9(rh50) X; muEx248. Description: muEx248 [daf-16p::GFP::DAF-16 cDNA + odr-1p::RFP]. Sterile at 25C; grow at 20C or less. Pick RFP+/GFP+ animals to maintain. Mutagen: Outcrossed: x Made by: C Kenyon/J Berman Received: 06/05/08 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2310 Species: Caenorhabditis elegans Genotype: kri-1(ok1251) I; glp-1(e2141) III; muEx353. Description: muEx353 [kri-1(cDNA, a-isoform)::GFP + odr-1p::RFP]. Pick RFP+ to maintain. Temperature sensitive. Sterile at 25C; maintain at 15-20C. Reference: Berman JR and Kenyon C. Cell. 2006 Mar 10;124(5):1055-68. Mutagen: Outcrossed: x0 Made by: Jennifer Berman Received: 05/02/13 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF237 Species: Caenorhabditis elegans Genotype: muIs2 unc-31(e169) IV. Description: muIs2 [mab-5::lacZ + unc-31(+)]. non-Unc. Mutagen: Integrated by gamma rays Outcrossed: x Made by: Steve Salser Received: 11/29/99 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF250 Species: Caenorhabditis elegans Genotype: cat-4(e1141) him-5(e1490) V. Description: Catecholamine abnormal. Throws males. Mutagen: Outcrossed: x Made by: Received: 06/17/04 University of San Diego ----------------------------------------------------------------------------- Strain: CF2570 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; daf-2(e1370) III; muIs142. Description: muIs142 [ges-1p::GFP::daf-16(cDNA) + odr-1p::RFP]. Maintain at 15-20C. Gamma irradiation-induced integration of muEx268. Rescues daf-16a1/c in the intestine (described in Libina et al, 2003). Reference: Zhang P, et al. Cell Metab, 2013. 17(1): p. 85-100. Mutagen: gamma irradiation Outcrossed: x4 Made by: Peichuan Zhang Received: 07/07/14 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF26 Species: C. elegans Genotype: lin-22(mu2) IV; him-5(e1490) V. Description: Alae-to-ray transformation similar to that of n372 animals. Mutagen: N/A Outcrossed: x0 Made by: Cynthia Kenyon Received: 06/21/17 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF263 Species: Caenorhabditis elegans Genotype: egl-20(mu39) IV. Description: QL migration defect. HSN migratin defect. Mutagen: EMS Outcrossed: x5 Made by: Jeanne Harris Received: 12/13/96 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2630 Species: Caenorhabditis elegans Genotype: sIs10314. Description: sIs10314 [rCesC06B3.4::GFP + pCeh361]. Strain was generated by outcrossing to remove dpy-5 from BC12544. Reference: Zhang P, et al. Cell Metab. 2013 Jan 8;17(1):85-100. Mutagen: X-ray Outcrossed: x2 Made by: Peichuan Zhang Received: 11/21/13 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF267 Species: Caenorhabditis elegans Genotype: muIs6 IV. Description: muIs6 [lin-39::lacZ + rol-6(su1006)] IV. lin-39 promoter driving lacZ. muIs6 was integrated by gamma irradiation. Mutagen: Gamma radiation Outcrossed: x3 Made by: Naomi Robinson Received: 03/09/01 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2805 Species: C. elegans Genotype: dop-2(vs105) V; dop-4(ok1321) dop-1(vs100) dop-3(vs106) X Description: Quadruple mutant removing four different dopamine receptor genes. Mutagen: N/A Outcrossed: x0 Made by: Marta Gaglia Received: 06/21/17 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2885 Species: Caenorhabditis elegans Genotype: aqp-1(tm2309) II. Description: Homozygous viable, but short-lived. Reference: Lee SJ, et al. Cell Metab. 2009 Nov;10(5):379-91. Mutagen: TMP/UV Outcrossed: x4 Made by: Seung-Jae Le Received: 09/27/16 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2892 Species: Caenorhabditis elegans Genotype: sEx10466. Description: sEx10466 [nnt-1p::GFP + (pCeh361)dpy-5(+)]. Pick GFP+ animals to maintain. Derived by outcrossing BC10466 2x to wild-type to remove dpy-5(e907). Reference: Zhang P, et al. Cell Metab. 2013 Jan 8;17(1):85-100. Mutagen: Outcrossed: x2 Made by: Peichuan Zhang Received: 09/27/16 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2893 Species: Caenorhabditis elegans Genotype: sEx11128. Description: sEx11128 [gpd-2p::GFP + (pCeh361)dpy-5(+)]. Pick GFP+ animals to maintain. Derived by outcrossing BC11128 2x to wild-type to remove dpy-5(e907). Reference: Zhang P, et al. Cell Metab. 2013 Jan 8;17(1):85-100. Mutagen: Outcrossed: x2 Made by: Peichuan Zhang Received: 09/27/16 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF2962 Species: Caenorhabditis elegans Genotype: muEx420. Description: muEx420 [hsp-12.6p::RFP(NLS) + odr-1p::RFP]. Pick RFP+ animals to maintain. Reference: Zhang P, et al. Cell Metab. 2013 Jan 8;17(1):85-100. Mutagen: Outcrossed: x0 Made by: Peichuan Zhang Received: 09/27/16 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF301 Species: Caenorhabditis elegans Genotype: mab-5(e2088) III; unc-31(e169) IV; him-5(e1490) V; muIs9 X. Description: muIs9 [hs-mab-5 + C14G10]. Heat-shock inducible mab-5. C14G10 contains a WT copy of unc-31. muIs9 integrated by gamma irradiation. Mutagen: Outcrossed: x Made by: Steve Salser Received: 03/09/01 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF31 Species: C. elegans Genotype: lin-22(mu5) IV; him-5(e1490) V. Description: Alae-to-ray transformation similar to that of n372 animals. Mutagen: N/A Outcrossed: x0 Made by: Cynthia Kenyon Received: 06/21/17 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF311 Species: Caenorhabditis elegans Genotype: mab-5(e1239) egl-5(n945) III; him-5(e1490) V. Description: Mutagen: Outcrossed: x Made by: Received: 06/17/04 University of San Diego ----------------------------------------------------------------------------- Strain: CF3166 Species: Caenorhabditis elegans Genotype: muEx473. Description: muEx473 [kin-19p::kin-19::tagRFP + tph-1p::GFP]. Pick RFP+ GFP+ animals to maintain. Low transmission rate. Translational fusion of KIN-19 displaying age-dependent aggregation. Reference: David DC, et al. PLoS Biol. 2010 Aug 10;8(8):e1000450. Mutagen: Outcrossed: x0 Made by: Della David Received: 12/06/16 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF32 Species: Caenorhabditis elegans Genotype: lin-1(e1026) IV; him-5(e1490) V. Description: Very slow growth. Mutagen: Outcrossed: x Made by: Cynthia Kenyon Received: 05/03/05 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF3556 Species: Caenorhabditis elegans Genotype: agIs6. Description: agIs6 [dod-24p::GFP]. Derived by outcrossing AU68 3 times to the Kenyon lab N2 strain. Reference: Yamawaki TM, et al. PLoS Biol. 2010 Aug 31;8(8). pii: e1000468. Mutagen: Outcrossed: x3 Made by: Tracy Yamawaki Received: 12/06/16 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF36 Species: C. elegans Genotype: lin-22(n372) unc-33(e204) IV. Description: Desorption Mutagen: N/A Outcrossed: x0 Made by: Lisa Wrischnik Received: 06/21/17 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF3649 Species: Caenorhabditis elegans Genotype: muIs209. Description: muIs209 [myo-3p::kin-19::tagRFP + tph-1p::GFP]. KIN-19::tagRFP aggregates with age in body-wall muscles. Animals have reduced thrashing compared to controls. Generated in N2 background. References: Huang YC, et al. Elife. 2019 May 3;8. pii: e43059. doi: 10.7554/eLife.43059. David DC, et al. PLoS Biol. 2010 Aug 10;8(8):e1000450. Mutagen: Outcrossed: x4 Made by: Della David Received: 10/01/19 Babraham Institute, Cambridge, UK ----------------------------------------------------------------------------- Strain: CF367 Species: Caenorhabditis elegans Genotype: mig-14(mu71) II. Description: QL descendants anterior, QR descendants slightly posterior to WT. HSN posterior (50%), BDU posterior (50%), DTCs make extra turns. Mild Egl. Male Mating: ME2. mig-14, mom-3, pvl-2, and let-553 are the same gene. Mutagen: EMS Outcrossed: x3 Made by: Lee Honigberg Received: 12/13/96 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF376 Species: Caenorhabditis elegans Genotype: bar-1(mu63) X. Description: Weak allele of bar-1. Mutagen: spont supp of pry-1(mu38) Outcrossed: x3 Made by: Julin Maloof Received: 06/25/99 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF391 Species: Caenorhabditis elegans Genotype: muIs13 V. Description: muIs13 [egl-5::lacZ + rol-6(su1006)]. Rollers. New stock received at CGC November 2001. Mutagen: Integrated by gamma rays Outcrossed: x Made by: Bruce Wang Received: 11/29/99 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF439 Species: Caenorhabditis elegans Genotype: lin-39(mu26) III; dpy-20(e1282) IV; him-5(e1490) V; muIs23. Description: muIs23 [hsp::lin-39 + (pMH86) dpy-20(+)]. Heat-shock inducible lin-39. muIs23 is a spontaneous integrant whose chromosomal location is unknown. [NOTE: This strain was previously described as carrying lin-39(n1760), but it is actually carrying the g to a substitution of mu26. Strain MT7255 has been confirmed to be carrying the a to t substitution of n1760.] Mutagen: Outcrossed: x Made by: Craig Hunter Received: 03/09/01 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF453 Species: Caenorhabditis elegans Genotype: muIs16 II; dpy-20(e1282) IV. Description: muIs16 [mab-5::GFP + dpy-20(+)]. non-Dpy. Mutagen: Integrated by gamma rays Outcrossed: x Made by: Hunter and Cowing Received: 11/29/99 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF4582 Species: Caenorhabditis elegans Genotype: muIs252 II; unc-119(ed3) III. Description: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Somatic expression of wrmScarlet1-10 (under the control of the eft-3 promoter and unc-54 3'UTR). Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628 Mutagen: Outcrossed: x1 Made by: Jerome Goudeau Received: 07/15/20 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF4586 Species: Caenorhabditis elegans Genotype: muIs252 II; unc-119(ed3) III; vha-13(mu493[wrmScarlet11::vha-13]) V. Description: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Homozygous viable. Endogenously-tagged wrmScarlet11::vha-13 generated via CRISPR/Cas9 insertion into parental strain CF4582. Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jerome Goudeau Received: 08/05/22 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF4587 Species: Caenorhabditis elegans Genotype: muIs253 II; unc-119(ed3) III. Description: muIs253 [eft-3p::sfGFP1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Somatic expression of sfGFP1-10 (under the control of the eft-3 promoter and the unc-54 3'UTR). [NOTE: A low level of "leaky" GFP expression from sfGFP1-10 is detected at high magnifications.] Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628 Mutagen: Outcrossed: x1 Made by: Jerome Goudeau Received: 07/15/20 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF4588 Species: Caenorhabditis elegans Genotype: muIs253 muIs252 II; unc-119(ed3) III. Description: muIs253 [eft-3p::sfGFP1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Somatic expression of sfGFP1-10 and wrmScarlet1-10 (both under the control of the eft-3 promoter and the unc-54 3'UTR). [NOTE: A low level of "leaky" GFP expression from sfGFP1-10 is detected at high magnifications.] Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628 Mutagen: Outcrossed: x1 Made by: Jerome Goudeau Received: 07/15/20 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF4592 Species: Caenorhabditis elegans Genotype: muIs253 II; unc-119(ed3) III; his-3(mu496[his-3::sfGFP11]) V. Description: muIs253 [eft-3p::sfGFP1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Somatic expression of sfGFP1-10 (under the control of the eft-3 promoter and the unc-54 3'UTR). GFP11 tag inserted into endogenous his-3 locus via CRISPR/Cas9 insertion into parental strain CF4587. Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jerome Goudeau Received: 05/16/23 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF4594 Species: Caenorhabditis elegans Genotype: muIs252 II; unc-119(ed3) III; his-3(mu497[his-3::wrmScarlet11]) V. Description: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Homozygous viable. Endogenously-tagged his-3::wrmScarlet11 generated via CRISPR/Cas9 insertion into parental strain CF4582. Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jerome Goudeau Received: 08/05/22 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF4601 Species: Caenorhabditis elegans Genotype: muIs252 II; unc-119(ed3) III; fib-1(mu498[wrmScarlet11::fib-1]) V. Description: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Homozygous viable. Endogenously-tagged wrmScarlet11::linker::fib-1 generated via CRISPR/Cas9 insertion into parental strain CF4582. Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jerome Goudeau Received: 08/05/22 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF4608 Species: Caenorhabditis elegans Genotype: muIs252 II; unc-119(ed3) III; his-3(mu500[his-3::wrmScarlet11(x3)]) V. Description: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Homozygous viable. Endogenously-tagged his-3::wrmScarlet11(x3) generated via CRISPR/Cas9 insertion of three wrmScarlet11 tags into the endogenous his-3 locus in parental strain CF4582. Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jerome Goudeau Received: 08/05/22 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF4610 Species: Caenorhabditis elegans Genotype: muIs257 I. Description: muIs257 [myo-3p::wrmScarlet1-10::unc-54 3'UTR] I. Muscle-specific expression of wrmScarlet1-10 (Under the control of the myo-3 promoter and unc-54 3'UTR). Generated using CRISPR/Cas9 in the SKI-LODGE strain WBM1126. Reference: Goudeau J, et al. bioRxiv 2020.07.02.185249; doi: https://doi.org/10.1101/2020.07.02.185249 Mutagen: Outcrossed: x1 Made by: Jerome Goudeau Received: 07/15/20 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF4611 Species: Caenorhabditis elegans Genotype: muIs257 I; fib-1(mu498[wrmScarlet11::fib-1]) V. Description: muIs257 [myo-3p::wrmScarlet1-10::unc-54 3'UTR] I. Homozygous viable. Endogenously-tagged wrmScarlet11::linker::fib-1 generated via CRISPR/Cas9 insertion into parental strain CF4610. Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jerome Goudeau Received: 08/05/22 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF4614 Species: Caenorhabditis elegans Genotype: muIs252 II; tbb-2(muIs260[wrmScarlet11::tbb-2]) unc-119(ed3) III. Description: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. split-wrmScarlet11 inserted at the N-terminus of the endogenous tbb-2 locus; detectable in all somatic tissues where wrmScarlet1-10 is present. Figure 3B from Goudeau et al., Genetics 2021. Reference: Goudeau J, et al. Genetics. 2021 Apr; 217(4): iyab014. PMID: 33693628. Mutagen: Outcrossed: x0 Made by: Jerome Goudeau Received: 08/05/22 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF4616 Species: Caenorhabditis elegans Genotype: muIs252 II; unc-119(ed3) III; vha-13(muIs264[wrmScarlet11(x2)::vha-13]) V. Description: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Two tandem repeats of split-wrmScarlet11 inserted at the N-terminus of the endogenous VHA-13 locus; detectable in somatic tissues where wrmScarlet1-10 is present. Figure 4A from Goudeau et al., Genetics 2021. Reference: Goudeau J, et al. Genetics. 2021 Apr; 217(4): iyab014. PMID: 33693628. Mutagen: Outcrossed: x1 Made by: Jerome Goudeau Received: 08/05/22 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF4625 Species: Caenorhabditis elegans Genotype: muIs252 II; unc-119(ed3) III; tomm-20(muIs276[tomm-20::wrmScarlet11(MDELYK)]) V. Description: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. split-wrmScarlet11(MDELYK) inserted at the C-terminus of the endogenous TOMM-20 locus; detectable in somatic tissues where wrmScarlet1-10 is present. Figure S6B from Goudeau et al., Genetics 2021. Reference: Goudeau J, et al. Genetics. 2021 Apr; 217(4): iyab014. PMID: 33693628. Mutagen: Outcrossed: x0 Made by: Jerome Goudeau Received: 08/05/22 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF4639 Species: Caenorhabditis elegans Genotype: glh-1(sam140[glh-1::T2A::wrmScarlet(1-10)]) I; fib-1(mu498[wrmScarlet11::fib-1]) V. Description: fib-1(mu498[wrmScarlet11::fib-1]) generated via CRISPR/Cas9 insertion into parental strain DUP237; transgene contains a linker between wrmScarlet11 and fib-1. Endogenous fib-1 detectable in the germline. T2A::wrmScarlet(1-10) fused to the C-terminus of endogenous GLH-1. The T2A self-cleaving peptide separates wrmScarlet(1-10) from GLH-1 post-translationally so that wrmScarlet(1-10) disperses throughout germ cell nuclei and cytoplasm. wrmScarlet(1-10) is also maternally loaded into embryos, where it persists through early and mid-embryonic development. Reference: Goudeau J, et al. bioRxiv 2020.07.02.185249; doi: https://doi.org/10.1101/2020.07.02.185249. Goudeau J, et al. Genetics. 2021 Apr; 217(4): iyab014. PMID: 33693628. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jerome Goudeau Received: 12/03/21 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF491 Species: Caenorhabditis elegans Genotype: pry-1(mu38) I; him-5(e1490) V. Description: Very sick, Muv, Scrawny. Extra rays in males in body. Ectopic expression of lin-39, mab-5, egl-5. Cold sensitive - grows better at 25C. Mutagen: EMS Outcrossed: x3 Made by: Julin Maloof Received: 06/25/99 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF512 Species: Caenorhabditis elegans Genotype: rrf-3(b26) II; fem-1(hc17) IV. Description: Grow at 15C. Sterile at 25C. Mutagen: Outcrossed: x Made by: H. Hsin Received: 03/17/04 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF579 Species: Caenorhabditis elegans Genotype: dpy-20(e1282) IV; him-5(e1490) V; muIs27. Description: muIs27 [mig-2::GFP + dpy-20(+)]. Him. non-Dpy. GFP is membrane enriched and expressed in many cells throughout development (see reference for details). Not known in which LG muIs27 is integrated. Mutagen: integrated w/ gamma rays Outcrossed: x1 Made by: Zipkin Received: 11/29/99 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF65 Species: C. elegans Genotype: mab-5(e2088) III; lin-22(n372) IV; him-5(e1490) V. Description: mab-5 mutation affects ectodermal and mesodermal lineages. lin-22 and him-5 mutations affect neuroblast formation from the epidermal precursor cell V5. Mutagen: N/A Outcrossed: x0 Made by: Alexander Kamb Received: 06/21/17 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF693 Species: Caenorhabditis elegans Genotype: unc-31(e169) IV; him-5(e1490) V; muIs28. Description: muIs28 [mig-2::GFP + unc-31(+)]. muIs28 is not mapped, but probably on LG II by process of elimination. Mutagen: Gamma Rays Outcrossed: x3 Made by: Lee Honigberg Received: 05/20/02 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF702 Species: Caenorhabditis elegans Genotype: muIs32 II. Description: muIs32 [mec-7p::GFP + lin-15(+)]. Superficially wild-type. Reference: Ch'ng, Q et al. (2003) Geneteics 164:1355-67. Mutagen: Outcrossed: x3 Made by: Q. Ch'ng / C. Kenyon Received: 03/04/10 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF716 Species: Caenorhabditis elegans Genotype: dpy-20(e1282) IV; mig-13(mu31) X; muIs37. Description: muIs37 [(pMS114) hsp::mig-13 + (pMH86) dpy-20(+)]. Inducible heat-shock promoter driven mig-13 rescues Q cell migration defects in mig-13(mu31) mutants. Reference: Sym, M., Robinson, N., and Kenyon, C. Cell. 1999 Jul 9;98(1):25-36. Mutagen: Gamma radiation Outcrossed: x Made by: Mary Sym Received: 11/21/13 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF726 Species: Caenorhabditis elegans Genotype: mig-13(mu225) X. Description: QR descendants fail to migrate anteriorly with 100% penetrance. BDUL/R fail to migrate anteriorly (incomplete penetrance). Mutagen: EMS Outcrossed: x3 Made by: Ch'ng and Sym Received: 09/23/99 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF80 Species: Caenorhabditis elegans Genotype: mab-3(mu15) II; him-5(e1490) V. Description: Abnormal V rays (male). Mutagen: Outcrossed: x Made by: D. Cowing/C. Kenyon Received: 10/22/09 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF816 Species: Caenorhabditis elegans Genotype: him-5(e14??) V; ref-2(mu218) X. Description: In males, P7.p and P8.p fail to fuse with hyp7 at the end of L1. Dominant. him-5 allele is either e1467 or e1490. Mutagen: EMS Outcrossed: x2 Made by: Scott Alper Received: 11/22/02 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF891 Species: Caenorhabditis elegans Genotype: dpy-20(e1282) IV; muIs37. Description: muIs37 [(pMS114) hsp::mig-13 + (pMH86) dpy-20(+)]. Inducible heat-shock promoter driven mig-13 rescues Q cell migration defects in mig-13(mu31) mutants. Reference: Sym, M., Robinson, N., and Kenyon, C. Cell. 1999 Jul 9;98(1):25-36. Mutagen: Outcrossed: x Made by: Mary Sym Received: 11/21/13 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF896 Species: Caenorhabditis elegans Genotype: dpy-20(e1282) IV; muIs42. Description: muIs42[mig-13::GFP + dpy-20(+)]. non-Dpy strain. GFP+ in ventral cord cells, pharyngeal-intestinal valve cells. Mutagen: gamma irradiation Outcrossed: x Made by: Mary Sym Received: 10/18/05 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF978 Species: Caenorhabditis elegans Genotype: unc-24(e138) IV; bar-1(mu349) X. Description: Unc. QL descendants in a mutant position (anterior of ALM). Okay to grow at 15 or 20C. Mutagen: EMS Outcrossed: x Made by: Jen Whangbo Received: 10/05/05 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CF980 Species: Caenorhabditis elegans Genotype: egl-20(n585) IV; bar-1(mu349) X. Description: Egl. QL descendants in a mutant position (anterior of ALM). Okay to grow at 15 or 20C. Mutagen: EMS Outcrossed: x Made by: Jen Whangbo Received: 10/05/05 Calico Life Sciences LLC ----------------------------------------------------------------------------- Strain: CFB2252 Species: Caenorhabditis brenneri Genotype: Caenorhabditis brenneri. Description: Male-female strain. Caenorhabditis brenneri reference genome. 107 generations of full-sib mating. Mutagen: N/A Outcrossed: x0 Made by: Charles F Baer Received: 09/19/23 University of Florida, Gainesville, FL ----------------------------------------------------------------------------- Strain: CFJ108 Species: C. elegans Genotype: kstSi60 II; unc-119(ed3) III. Description: kstSi60 [LoxP + Cbr-unc-119(+) + LoxP + mlc-2p::GFP(kst32)] II. N2-like, no MLC-2::GFP fluorescence. mlc-2p::GFP(kst32) is a partial, non-functional GFP reporter used for section in MosTI, an updated technique for targeted single-copy and extra-chromosomal array insertion. Cbr-unc-119(+) is flanked by LoxP sites, facilitating removal by recombination. Reference: El Mouridi S, et al. 2022. Mutagen: Outcrossed: x0 Made by: Sonia El Mouridi Received: 02/28/22 King Abduallah University of Science and Technology (KAUST) ----------------------------------------------------------------------------- Strain: CFJ111 Species: C. elegans Genotype: kstSi61 II; unc-119(ed3) III. Description: kstSi61 [LoxP + Cbr-unc-119(+) + LoxP + hygroR(kst31)] II. N2-like, no hygromycin resistance (HygroR). hygroR(kst31) is a partial, non-functional hygromycin-resistance construct used for section in MosTI, an updated technique for targeted single-copy and extra-chromosomal array insertion. Cbr-unc-119(+) is flanked by LoxP sites, facilitating removal by recombination. Reference: El Mouridi S, et al. 2022. Mutagen: Outcrossed: x0 Made by: Sonia El Mouridi Received: 02/28/22 King Abduallah University of Science and Technology (KAUST) ----------------------------------------------------------------------------- Strain: CFJ184 Species: C. elegans Genotype: kstSi84 I; unc-119(ed3) III. Description: kstSi84 [LoxP + Cbr-unc-119(+) + LoxP + mlc-2p::GFP(kst32)] I. N2-like, no MLC-2::GFP fluorescence. mlc-2p::GFP(kst32) is a partial, non-functional GFP reporter used for section in MosTI, an updated technique for targeted single-copy and extra-chromosomal array insertion. Cbr-unc-119(+) is flanked by LoxP sites, facilitating removal by recombination. Reference: El Mouridi S, et al. 2022. Mutagen: Outcrossed: x0 Made by: Sonia El Mouridi Received: 02/28/22 King Abduallah University of Science and Technology (KAUST) ----------------------------------------------------------------------------- Strain: CFJ191 Species: C. elegans Genotype: kstSi32 I; unc-119(ed3) III; kstEx45. Description: kstSi32 [Cbr-unc-119(kst13)] I. kstEx45 [hsp-16.41p::Cas9::gpd-2::TagRFP-T::smu-1 3'UTR + mlc-1p::mCherry + NeoR]. Pick mCherry+ to maintain. Unc. Cbr-unc-119(kst13) is a partial unc-119 used for section in MosTI, an updated technique for targeted single-copy and extra-chromosomal array insertion. Reference: El Mouridi S, et al. 2022. Mutagen: Outcrossed: x0 Made by: Sonia El Mouridi Received: 02/28/22 King Abduallah University of Science and Technology (KAUST) ----------------------------------------------------------------------------- Strain: CFJ192 Species: C. elegans Genotype: unc-119(ed3) III; kstSi37 IV; kstEx46. Description: kstSi37 [Cbr-unc-119(kst13)] IV. kstEx46 [hsp-16.41p::Cas9::gpd-2::TagRFP-T::smu-1 3'UTR + mlc-1p::mCherry + NeoR]. Pick mCherry+ to maintain. Unc. Cbr-unc-119(kst13) is a partial unc-119 used for section in MosTI, an updated technique for targeted single-copy and extra-chromosomal array insertion. Reference: El Mouridi S, et al. 2022. Mutagen: Outcrossed: x0 Made by: Sonia El Mouridi Received: 02/28/22 King Abduallah University of Science and Technology (KAUST) ----------------------------------------------------------------------------- Strain: CFJ302 Species: C. elegans Genotype: unc-119(kst33) III. Description: Maintain at 15C. Temperature-sensitive unc-119 allele. Wild-type at 15C, intermediate Unc and Egl at 20C, and fully penetrant Unc and Egl at 25C. For use in transgenesis, maintain the strain at lower temperatures for increased brood size and easier injection, then transfer animals to 25C to select for transgenic animals based on Unc rescue. Molecular characterization shows a complex allele with a 210 bp duplication from a nearby exon-intron junction, which introduces 12 amino acids and a putative splice donor at a consensus splice acceptor site. The phenotype is most likely caused by temperature-sensitive splicing defects based on RT-PCR. Reference: Aljohani M, et al. Arrayed oligo libraries: genome-wide DNA- and RNP-based platforms for templated and non-templated CRISPR-Cas9 editing in C. elegans. (Submitted) Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Mohammed Aljohani Received: 06/05/24 King Abduallah University of Science and Technology (KAUST) ----------------------------------------------------------------------------- Strain: CFJ42 Species: C. elegans Genotype: kstSi42 II; unc-119(ed3) III. Description: kstSi42 [Cbr-unc-119(kst13)] II. Unc. Cbr-unc-119(kst13) is a partial unc-119 used for section in MosTI, an updated technique for targeted single-copy and extra-chromosomal array insertion. Reference: El Mouridi S, et al. 2022. Mutagen: Outcrossed: x0 Made by: Sonia El Mouridi Received: 02/28/22 King Abduallah University of Science and Technology (KAUST) ----------------------------------------------------------------------------- Strain: CFJ77 Species: C. elegans Genotype: kstSi32 I; unc-119(ed3) III. Description: kstSi32 [Cbr-unc-119(kst13)] I. Unc. Cbr-unc-119(kst13) is a partial unc-119 used for section in MosTI, an updated technique for targeted single-copy and extra-chromosomal array insertion. Reference: El Mouridi S, et al. 2022. Mutagen: Outcrossed: x0 Made by: Sonia El Mouridi Received: 02/28/22 King Abduallah University of Science and Technology (KAUST) ----------------------------------------------------------------------------- Strain: CFJ94 Species: C. elegans Genotype: unc-119(ed3) III; kstSi37 IV. Description: kstSi37 [Cbr-unc-119(kst13)] IV. Unc. Cbr-unc-119(kst13) is a partial unc-119 used for section in MosTI, an updated technique for targeted single-copy and extra-chromosomal array insertion. Reference: El Mouridi S, et al. 2022. Mutagen: Outcrossed: x0 Made by: Sonia El Mouridi Received: 02/28/22 King Abduallah University of Science and Technology (KAUST) ----------------------------------------------------------------------------- Strain: CG1 Species: Caenorhabditis elegans Genotype: lev-11(rg1) I; him-5(e1490) V. Description: Resistant to levamisole. Egl-d. Twitch at 25C. Long. Throws males. Mutagen: EMS Outcrossed: x3 Made by: Rene Garcia Received: 04/10/07 Texas A&M University, College Station, TX ----------------------------------------------------------------------------- Strain: CG118 Species: Caenorhabditis elegans Genotype: unc-43(sy574) IV; him-5(e1490) V. Description: Males constitutively protract their spicules in the absence of mating cues. Mutagen: EMS Outcrossed: x5 Made by: Brigitte LeBoeuf Received: 07/15/08 Texas A&M University, College Station, TX ----------------------------------------------------------------------------- Strain: CG1367 Species: C.elegans Genotype: pck-2(rg551[pck-2::YFP]) I; him-5(e1490) V. Description: rg551 is an endogenous genomic CRISPR/Cas9 knock in of YFP fused to the C-terminus of the gene pck-2 in N2 background. Robust YFP fluorescence body wall muscle, intestine, hypodermis and pharyngeal muscle. Him. Reference: Goncalves J, et al. iScience 2020 Mar 19;23(4):100990. PMID: 32240955 Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: L. Rene Garcia Received: 09/08/20 Texas A&M University, College Station, TX ----------------------------------------------------------------------------- Strain: CG1428 Species: Caenorhabditis elegans Genotype: tmc-1(rg1003) X. Description: rg1003 promotes development and male mating behavior on a chemically defined C. elegans maintenance medium (CeMM). Reference: Zhang L, et al. Nature Communications (2015) In press. Mutagen: EMS Outcrossed: x4 Made by: Liusuo Zhang Received: 02/13/15 Texas A&M University, College Station, TX ----------------------------------------------------------------------------- Strain: CG1438 Species: C. elegans Genotype: egl-2(rg444) him-5(e1490) V. Description: rg444 is an endogenous genomic CRISPR/Cas9 knock in of YFP fused to the C-terminus of the EGL-2 K+ Channel in N2 background. Faint YFP fluorescent puncta can be detected on muscle membranes and neural cell bodies and processes at high power magnification in all stages. Him. Reference: Goncalves J, et al. iScience 2020 Mar 19;23(4):100990. PMID: 32240955 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: L. Rene Garcia Received: 09/08/20 Texas A&M University, College Station, TX ----------------------------------------------------------------------------- Strain: CG197 Species: Caenorhabditis elegans Genotype: egl-2(rg4) him-5(e1490) V. Description: No longer able to suppress male muscle seizures in the absence of food. Mutagen: TMP Outcrossed: x2 Made by: L. Rene Garcia Received: 07/15/08 Texas A&M University, College Station, TX ----------------------------------------------------------------------------- Strain: CG21 Species: Caenorhabditis elegans Genotype: egl-30(tg26) I; him-5(e1490) V. Description: Hyperactive. Small. Spicule protruder. Coiler. Throws males. Mutagen: Outcrossed: x3 Made by: Rene Garcia Received: 04/10/07 Texas A&M University, College Station, TX ----------------------------------------------------------------------------- Strain: CG289 Species: Caenorhabditis elegans Genotype: unc-103(n1213) pha-1(e2123) III; him-5(e1490) V;rgEx81. Description: rgEx81[pha-1(+) + unc-103 promoter E::unc-103 genomic DNA]. Maintain at 20C. Mutagen: Outcrossed: x Made by: Rene Garcia Received: 10/10/08 Texas A&M University, College Station, TX ----------------------------------------------------------------------------- Strain: CG460 Species: Caenorhabditis elegans Genotype: unc-43(sy574) IV; him-5(e1490) V; rgEx161. Description: rgEx161[lev-11p::UNC-43g + gtl-1p::CFP]. rgEx161 rescues unc-43(sy574)-induced spicule protraction. Mutagen: Outcrossed: x Made by: Brigitte LeBoeuf Received: 07/15/08 Texas A&M University, College Station, TX ----------------------------------------------------------------------------- Strain: CG490 Species: Caenorhabditis elegans Genotype: unc-103(n1213) III; egl-2(rg4) him-5(e1490) V; rgEx173. Description: rgEx173 [unc-103ep::egl-2(cDNA) + gtl-1p::CFP]. Pick animals with cyan fluorescence in their intestines. rgEx173 rescues food deprivation's ability to suppress unc-103(n1213)-induced male sex muscle seizures. Mutagen: Outcrossed: x Made by: Todd Gruninger Received: 07/15/08 Texas A&M University, College Station, TX ----------------------------------------------------------------------------- Strain: CG499 Species: Caenorhabditis elegans Genotype: daf-2(e1368) unc-103(n1213) III; him-5(e1490) V; rgEx178. Description: rgEx178 [lev-11p::daf-2(+) + lev-11p::GFP]. rgEx178 rescues food deprivation suppression of unc-103(n1213)-induced spicule protraction. Mutagen: Outcrossed: x Made by: Todd Gruninger Received: 07/15/08 Texas A&M University, College Station, TX ----------------------------------------------------------------------------- Strain: CG500 Species: Caenorhabditis elegans Genotype: daf-2(e1368) unc-103(n1213) III; him-5(e1490) V; rgEx180. Description: rgEx180 [aex-3p::daf-2(+) + tnt-4p::GFP]. rgEx180 reduces unc-103(n1213)-induced spicule protraction, and rescues constitutive dauer formation. Mutagen: Outcrossed: x Made by: Todd Gruninger Received: 07/15/08 Texas A&M University, College Station, TX ----------------------------------------------------------------------------- Strain: CG625 Species: Caenorhabditis elegans Genotype: unc-103(n1213) pha-1(e2123) III; him-5(e1490) V; rgEx235. Description: rgEx235 [plc-3p::YFP + pha-1(+)]. Expresses YFP from a 4.4bk upstream plc-3 promoter. Maintain at 20C. Mutagen: Outcrossed: x Made by: Todd Gruninger Received: 07/15/08 Texas A&M University, College Station, TX ----------------------------------------------------------------------------- Strain: CG640 Species: Caenorhabditis elegans Genotype: daf-2(e1368) unc-103(n1213) III; him-5(e1490) V; rgEx247. Description: rgEx247 [hsp-16p::daf-2(+) + lev-11p::GFP]. rgEx247 restores food-deprivation suppression of unc-103(n1213)-induced spicule protraction. Mutagen: Outcrossed: x Made by: Todd Gruninger Received: 07/15/08 Texas A&M University, College Station, TX ----------------------------------------------------------------------------- Strain: CG644 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; him-5(e1490) V; rgEx251. Description: rgEx251[pha-1(+) + osm-12 promoter:: unc-103(gf)]. Maintain at 20C. Mutagen: Outcrossed: x Made by: Todd Gruninger Received: 10/10/08 Texas A&M University, College Station, TX ----------------------------------------------------------------------------- Strain: CGC1 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: CGC1 (formerly known as PD1074) is intended to be used as a wild-type reference strain with the closely matched genome assembly of Yoshimura, et al. (Genome Res. 2019 Jun;29(6):1009-1022) available on Wormbase as VC2010-1.0. (ENA study accession PRJEB28388; assembly accession GCA_900538205). CGC1 is a defined and recently cloned population of animals derived from the original "Bristol" variant of C. elegans originally obtained by Brenner from E. Dougherty with no known history of mutagenesis. Brenner's original population, called N2, was used as the basis for the vast majority of laboratory strains in use currently. No early frozen stock of the unmutagenized N2 population currently exists, but later stocks were available from several laboratories. CGC1 is a clonal population founded by picking a single worm of one such stock, VC3510. VC3510 in turn derives from a subpopulation of N2 described in the literature as VC2010. We note that CGC1 is expected to be largely similar to most lab N2 strains, but that as a clonal isolate derived from N2, there will be some loci that will vary compared to any other particular N2 isolate. One such example is a partial deletion of the alh-2 locus in CGC1. Additional loci that were found to vary between the prior N2 reference genome (WormBase release WS264) and the VC2010-1.0 assembly are detailed in supplemental table 8 in Yoshimura, et al, (2019). Mutagen: N/A Outcrossed: x0 Made by: Karen Artiles & Mark Edgley Received: 01/01/19 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: CGC10 Species: Caenorhabditis elegans Genotype: umnTi7 IV. Description: umnTi7 [eft-3p::GFP + unc-119(+)] IV. Integration site: (IV:+14.35 cM/nt 15,997,713). Might still contain unc-119(ed3) in background. Mutagen: MosSCI Outcrossed: x Made by: Britt Werre Received: 04/03/15 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC102 Species: C. elegans Genotype: mir-61(umn14[lox2272 + myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR Lox511I + Lox2272]) V. Description: mir-61 pre-miRNA deletion strain deletion allele in which mir-61 pre-miRNA was replaced by myo-2p::wrmScarlet. Generated in parental strain N2. Rollers. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.] Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott & Marcus Vargas Received: 04/23/19 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC103 Species: C. elegans Genotype: mir-61(umn15[lox2272 myo-2p::wrmScarlet + lox511I + Lox2272]) V. Description: mir-61(umn15[lox2272 myo-2p::wrmScarlet + lox511I + Lox2272]) V. Derived by CRE-meditated excision of SEC in parental strain CGC102, leaving myo-2p::wrmScarlet. Mutagen: CRE Outcrossed: x0 Made by: Julie Knott & Marcus Vargas Received: 04/19/19 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC104 Species: C. elegans Genotype: mir-61(umn16[lox2272]) V. Description: Derived by CRE-meditated excision of SEC and myo-2p::wrmScarlet in parental strain CGC102, leaving disrupted mir-61 locus. Mutagen: CRE Outcrossed: x0 Made by: Julie Knott & Marcus Vargas Received: 04/19/19 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC105 Species: C. elegans Genotype: hIn1 [umnIs78] I. Description: umnIs78 [myo-2p::mKate2 + NeoR, I: 12541645 (intergenic)] I. Superficially wild-type. Crossover suppressor for LGI right. Inversion includes unc-75 and unc-54. Derived by insertion of myo-2p::mKate2 transgene into hIn1 inversion in parental strain KR1949 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott Received: 03/05/19 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC11 Species: Caenorhabditis elegans Genotype: unc-5(e53)/nT1 [umnIs1] IV; dpy-11(e224)/nT1 V. Description: umnIs1 [eft-3p::GFP + HygroR, V:~2821000] V. umnIs1 GFP is expressed at low levels, and might be difficult to see in heterozygotes. Heterozygotes are WT and segregate WT, DpyUnc, Vul and dead eggs. Maintain by picking GFP+ WT. Derived by insertion of GFP transgene into parental strain MT1000 using MosSCI. Mutagen: MosSCI Outcrossed: x Made by: Julie Knott Received: 03/27/15 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC110 Species: C. elegans Genotype: mir-250(umn21[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR Lox511I + Lox2272)] V. Description: mir-250 pre-miRNA deletion strain deletion allele in which mir-250 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.] Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott & Marcus Vargas Received: 05/13/19 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC111 Species: C. elegans Genotype: mir-250(umn22[lox2272 myo-2p::wrmScarlet + lox511I + Lox2272)] V. Description: Derived by CRE-meditated excision of SEC in parental strain CGC110, leaving myo-2p::wrmScarlet. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott & Marcus Vargas Received: 05/13/19 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC112 Species: C. elegans Genotype: mir-250(umn23[lox2272]) V. Description: Derived by CRE-meditated excision of SEC and myo-2p::wrmScarlet in parental strain CGC110, leaving disrupted mir-250 locus. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott & Marcus Vargas Received: 05/13/19 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC113 Species: C. elegans Genotype: mir-61&mir-250(umn24[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR Lox511I + Lox2272)] V. Description: mir-61&mir-250 pre-miRNA deletion strain deletion allele in which mir-61&mir-250 pre-miRNAs were replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.] Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott & Marcus Vargas Received: 06/06/19 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC114 Species: C. elegans Genotype: mir-61&mir-250(umn25[lox2272 myo-2p::wrmScarlet + lox511I + Lox2272)] V. Description: Derived by CRE-meditated excision of SEC in parental strain CGC113, leaving myo-2p::wrmScarlet. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott & Marcus Vargas Received: 06/06/19 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC115 Species: C. elegans Genotype: mir-61&mir-250(umn26[lox2272]) V. Description: Derived by CRE-meditated excision of SEC and myo-2p::wrmScarlet in parental strain CGC113, leaving disrupted mir-61&mir-250 loci. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott & Marcus Vargas Received: 06/06/19 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC12 Species: Caenorhabditis elegans Genotype: umnIs2 V. Description: umnIs2 [eft-3p::NLS::tdTomato + HygroR, V:~2821000] V. Derived by insertion of tdTomato transgene into parental strain N2 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 03/27/15 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC120 Species: C. elegans Genotype: mir-792(umn31[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR Lox511I + Lox2272]) V. Description: mir-792 pre-miRNA deletion strain deletion allele in which mir-792 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.] Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott & Marcus Vargas Received: 07/30/19 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CGC121 Species: C. elegans Genotype: mir-785(umn32[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR Lox511I + Lox2272]) X. Description: mir-785 pre-miRNA deletion strain deletion allele in which mir-785 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.] Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott & Marcus Vargas Received: 08/17/19 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC122 Species: C. elegans Genotype: mir-392(umn33[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR Lox511I + Lox2272]) X. Description: mir-392 pre-miRNA deletion strain deletion allele in which mir-392 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.] Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott & Marcus Vargas Received: 09/19/19 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC123 Species: C. elegans Genotype: mir-57(umn34[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR LoX511I + Lox2272]) II. Description: mir-57 pre-miRNA deletion strain deletion allele in which mir-57 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.] Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott & Marcus Vargas Received: 09/19/19 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC128 Species: C. elegans Genotype: +/hT2 [umnIs15] I; dcr-1(pk1351)/hT2 [bli-4(e937) let-?(h661)] III. Description: umnIs15 [myo-2p::GFP + NeoR, III: 9421936 (intergenic)] I. Heterozygotes are WT GFP+ and segregate WT GFP+, dcr-1 homozygotes (protruding vulva, sterile/egl, rupture at vulva), lethal GFP+ hT2 homozygotes (arrest stage unknown) and dead eggs (aneuploids). Will throw an occasional GFP+ Pvul. Pick WT GFP+ and check for correct segregation of progeny to maintain. Derived from parental strains CGC26 and NL687. [NOTE: 3/1995: Apparently the lethal mutation is closely linked but not within the balanced region of hT2. It can occasionally recombine away so that the strain will segregate Bli-4 hT2 homozygotes. (Mark Edgley)] Mutagen: Outcrossed: x0 Made by: Aric Daul Received: 11/07/19 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC13 Species: Caenorhabditis elegans Genotype: unc-5(e53)/nT1 [umnIs3] IV; dpy-11(e224)/nT1 V. Description: umnIs3 [eft-3p::NLS::tdTomato + HygroR, V:~2821000] IV. tdTomato is expressed at low levels, and might be difficult to see in heterozygotes. Heterozygotes are WT and segregate WT, DpyUnc, Vul and dead eggs. Maintain by picking WT with tdTomato expression. Derived by insertion of tdTomato transgene into parental strain MT1000 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 07/25/15 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC131 Species: C. elegans Genotype: mir-248(umn41[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR LoX511I + Lox2272]) X. Description: mir-248 pre-miRNA deletion allele in which mir-248 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.] Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott & Marcus Vargas Received: 12/06/19 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC132 Species: C. elegans Genotype: mir-356(umn42[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR LoX511I + Lox2272]) III. Description: mir-356 pre-miRNA deletion strain deletion allele in which mir-356 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.] Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott & Marcus Vargas Received: 12/06/19 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC135 Species: C. elegans Genotype: let-7(umn45[let-7p::egl-13-NLS::mScarlet-I::c-myc-NLS::linker::mODC(422-461)(E428A/E430A/E431A)::let-858 3' UTR])/tmC24 [F23D12.4(tmIs1240) unc-9(tm9719)] X. Description: tmIs1240 [myo-2p::venus, X: F23D12.4] X. Nuclear mScarlet-I fused to a PEST was inserted in place of the endogenous let-7 pre-miRNA via CRISPR/CAS9. Heterozygotes are wild-type GFP+ mScarlet+ and segregate wild-type GFP+ mScarlet+ heterozygotes, mScarlet+ non-GFP dead larvae (umn45 homozygotes) and Mec(Unc) non-mScarlet GFP+ (tmC24 homozygotes). Maintain by picking wild-type GFP+ mScarlet+. Left Flanking: GCAAGCAGGCGATTGGTGGACGGTC, Right Flanking: AGCTGCGTCGTCTTGCTCTCACAAc. sgRNA: AAAATTGCATAGTTCACCGG. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott & Marcus Vargas Received: 08/08/20 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC136 Species: C. elegans Genotype: mir-84(umn46[mir-84p+SL1::egl-13-NLS::mScarlet-I::c-myc-NLS::linker::mODC(422-461)(E428A/E430A/E431A)::let-858 3' UTR]) X. Description: Nuclear mScarlet-I fused to a PEST was inserted in place of the endogenous mir-84 pre-miRNA via CRISPR/CAS9. Left Flanking: GTTGAGACATGTATATGTTTTTGTT, Right Flanking: GCTACTATTCATCATACGTCTGCCT. sgRNA: ATTCATCATACGTCTGCCTG. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Marcus Vargas & Julie Knott Received: 08/10/20 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC137 Species: C. elegans Genotype: mir-241(umn47[mir-241p+SL1::egl-13-NLS::mScarlet-I::c-myc-NLS::linker::mODC(422-461)(E428A/E430A/E431A)::let-858 3' UTR]) V. Description: Nuclear mScarlet-I fused to a PEST was inserted in place of the endogenous mir-241 pre-miRNA via CRISPR/CAS9. Left Flanking: CTATTTTTTTCACTTGGATTAGGGG, Right Flanking: GGGATGCTCTTTTTGTACCAAACCG. sgRNA: CCTCAACTTTGACACCCCCG. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Marcus Vargas & Julie Knott Received: 08/10/20 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC138 Species: C. elegans Genotype: unc-13(e51)/hT1 I; dpy-11(e224)/hT1 [unc-42(e270) umnIs79] V. Description: umnIs79 [myo-2p::GFP + NeoR, I: 6284001] I. Pick wild-type GFP+ to maintain. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, DpyUnc, arrested hT1 homozygotes (GFP+), and dead eggs. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into hT1 balancer in parental strain KR1037 using CRISPR/Cas9. Mutagen: Outcrossed: x Made by: Julie Knott & Marcus Vargas Received: 08/08/20 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC139 Species: C. elegans Genotype: unc-13(e51)/hT1 I; dpy-11(e224)/hT1 [unc-42(e270) umnIs80] V. Description: umnIs80 [myo-2p::mKate2 + NeoR, I: 6284001] I. Pick wild-type mKate2+ to maintain. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc, arrested hT1 homozygotes (mKate2+), and dead eggs. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate transgene into hT1 balancer in parental strain KR1037 using CRISPR/Cas9. Mutagen: Outcrossed: x Made by: Julie Knott & Marcus Vargas Received: 08/08/20 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC140 Species: C. elegans Genotype: goa-1(n499)/tmC20 [unc-14(tmIs1219) dpy-5(tm9715)] I. Description: This strain is difficult and time consuming to maintain. Gives relatively few heterozygotes. Homozygous lethal mutation balanced by Dpy- and myo-2p::Venus-marked inversion. Heterozygotes are paralyzed Unc and Egl with relatively dim pharyngeal GFP (Venus) expression. Heterozygotes segregate heterozygous non-Dpy GFP+ paralyzed Unc and Egl, non-GFP embryonic lethal (homozygous n499), and Dpy with brighter GFP+ (tmC20 homozygous). Remove Dpy from plate to prevent them from taking over. Heterozygotes tend to stack up in parallel clumps. Populations can be enriched by transferring these clumps to new plates and allowing Dpy (tmC20 homozygotes) to crawl out into bacterial lawn, and then picking away Dpy or transferring the clump of Hets to another plate. Derived by balancing n499 from parental strain MT1102 over tmC20 from FX30179. Mutagen: Outcrossed: x2 Made by: Aric Daul Received: 01/20/21 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC141 Species: C. elegans Genotype: mir-1821(umn48[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR LoX511I + Lox2272]) V. Description: mir-1821 pre-miRNA deletion allele in which mir-1821 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.] Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott & Marcus Vargas Received: 03/15/21 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC142 Species: C. elegans Genotype: mir-359(umn49[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR LoX511I + Lox2272]) V. Description: mir-359 pre-miRNA deletion allele in which mir-359 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.] Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott & Marcus Vargas Received: 03/16/21 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC143 Species: C. elegans Genotype: mir-1021(umn50[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR LoX511I + Lox2272]) IV. Description: mir-1021 pre-miRNA deletion allele in which mir-1021 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.] Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott & Marcus Vargas Received: 05/13/21 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC144 Species: C. elegans Genotype: mir-1022(umn51[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR LoX511I + Lox2272]) X. Description: mir-1022 pre-miRNA deletion allele in which mir-1022 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.] Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott & Marcus Vargas Received: 05/13/21 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC145 Species: C. elegans Genotype: mir-1824(umn52[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR LoX511I + Lox2272]) X. Description: mir-1824 pre-miRNA deletion allele in which mir-1824 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.] Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott & Marcus Vargas Received: 06/14/21 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC146 Species: C. elegans Genotype: mir-800(umn53[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR LoX511I + Lox2272]) X. Description: mir-800 pre-miRNA deletion allele in which mir-800 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.] Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 07/26/21 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC147 Species: C. elegans Genotype: mir-1818(umn54[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR LoX511I + Lox2272])I. Description: mir-1818 pre-miRNA deletion allele in which mir-1818 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.] Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 07/26/21 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC148 Species: C. elegans Genotype: mir-47(umn55[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR LoX511I + Lox2272])I. Description: mir-47 pre-miRNA deletion allele in which mir-47 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.] Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 07/26/21 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC149 Species: C. elegans Genotype: mir-81(umn56[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR LoX511I + Lox2272])I. Description: mir-81 pre-miRNA deletion allele in which mir-81 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.] Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 07/26/21 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC15 Species: Caenorhabditis elegans Genotype: umnIs4 III. Description: umnIs4 [eft-3p::NLS::tdTomato + HygroR, III:~5753000 (intergenic)] III. Derived by insertion of tdTomato transgene into parental strain N2 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 02/12/16 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC150 Species: C. elegans Genotype: mir-1829.3&F39B1.3(umn57[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR LoX511I + Lox2272])X. Description: mir-1829.3 pre-miRNA & F39B1.3 deletion allele in which mir-1829.3 pre-miRNA & F39B1.3 was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.] Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 07/26/21 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC151 Species: C. elegans Genotype: mir-1829.2(umn58[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR LoX511I + Lox2272]) X. Description: mir-1829.2 pre-miRNA deletion allele in which mir-1829.2 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.] Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 07/26/21 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC152 Species: C. elegans Genotype: mir-48(umn59[mir-48p+SL1::EGL-13NLS::mScarlet-I::cMycNLS::Lox511I::let-858 3'UTR]) V. Description: Nuclear mScarlet-I was inserted in place of the endogenous mir-48 pre-miRNA via CRISPR/CAS9.  Left Flanking: CACAGGTAAGTCAATTAACCAATTG, Right Flanking: TTATTATTATGTTTCATTCAATAAC. sgRNA: GGGAATGCGAGCTAGGCTGG. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott & Marcus Vargas Received: 09/15/21 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC153 Species: C. elegans Genotype: mir-48(umn60[mir-48p+SL1::EGL-13NLS::mScarlet-I::cMycNLS::linker::mODC(422-461)(E428A/E430A/E431A):: lox511I::let-858 3'UTR]) V. Description: Nuclear mScarlet-I was inserted in place of the endogenous mir-48 pre-miRNA via CRISPR/CAS9.  Left Flanking: CACAGGTAAGTCAATTAACCAATTG, Right Flanking: TTATTATTATGTTTCATTCAATAAC. sgRNA: GGGAATGCGAGCTAGGCTGG. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott & Marcus Vargas Received: 09/15/21 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC154 Species: C. elegans Genotype: mir-4812(umn61[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR LoX511I + Lox2272]) X. Description: mir-4812 pre-miRNA deletion allele in which mir-1824 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.] Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott Received: 09/28/21 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC159 Species: C. elegans Genotype: mir-61&mir-250(umn66[mir-61p::SL1::EGL-13NLS::lox2272::mScarlet-I::cMycNLS::Lox511I::let-858 3'UTR::lox2722]) II. Description: mScarlet replacement of mir-61 and mir-250 pre-miRNAs. SEC has been removed, leaving the SL1::EGL-13NLS::lox2272::mScarlet-I::cMycNLS::let-858 3'UTR transcriptional reporter in the locus Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Marcus Vargas & Julie Knott Received: 12/03/21 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CGC16 Species: Caenorhabditis elegans Genotype: hT2 [umnIs5] I; hT2 [bli-4(e937)] III. Description: umnIs5 [eft-3p::NLS::tdTomato + HygroR, III:~5753000 (intergenic)] I. Homozygous-viable translocation marked with bli-4 and tdTomato. tdTomato is expressed at low levels, and might be difficult to see in heterozygotes. Derived by insertion of tdTomato transgene into parental strain KR1234 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 01/14/16 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC169 Species: C. elegans Genotype: mir-788(umn76[mir-788p+SL1::EGL13NLS::lox2272]) X. Description: Deletion of mir-788 pre-miRNA via CRISPR/CAS9 and CRE/lox. Left Flanking: TCTGTGCGTATTACAAATTTTCAGCTGGAA, Right Flanking: GAATAGCAGTTTTCAAAATTGTGAGTTGCT. sgRNA: CTGCAAATGGAAGTTAGAAG. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott and Marcus L. Vargas Received: 03/01/22 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC17 Species: Caenorhabditis elegans Genotype: unc-4(e120)/mT1 [umnIs6] II; dpy-17(e164)/mT1 [dpy-10(e128)] III. Description: umnIs6 [eft-3p::NLS::tdTomato + HygroR, III:~5753000 (intergenic)] II. Heterozygotes are WT with dim red fluorescence, and segregate WT with dim red fluorescence, arrested mT1 aneuploids, sterile Dpys (mT1 homozygotes with more intense red fluorescence), and DpyUnc with no red fluorescence. Pick WT with dim red fluorescence and check for correct segregation of progeny to maintain. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 04/22/16 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC170 Species: C. elegans Genotype: mir-788(umn77[mir-788p+SL1::EGL13NLS::lox2272::mScarlet-I::cMycNLS::let-858 3' UTR::lox2272]) X. Description: Nuclear mScarlet-I was inserted in place of the endogenous mir-788 pre-miRNA via CRISPR/CAS9. Left Flanking: TCTGTGCGTATTACAAATTTTCAGCTGGAA, Right Flanking: GAATAGCAGTTTTCAAAATTGTGAGTTGCT. sgRNA: CTGCAAATGGAAGTTAGAAG. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott and Marcus L. Vargas Received: 03/01/22 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC171 Species: C. elegans Genotype: mir-799(umn78[mir-799p+SL1::EGL13NLS::lox2272]) X. Description: Deletion of mir-799 pre-miRNA via CRISPR/CAS9 and CRE/lox. Left Flanking: ATTTTCTATTTATTGGTATAAAATATGTTA, Right Flanking: AAGAAGTACACTTCATATGCTCCTAACAAT. sgRNA: GTGAACCCTGATAAAGCTAG. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott and Marcus L. Vargas Received: 02/21/22 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC172 Species: C. elegans Genotype: mir-799(umn79[mir-799p+SL1::EGL13NLS::lox2272mScarlet-I::cMycNLS::let-858 3' UTR::lox2272]) X. Description: Nuclear mScarlet-I was inserted in place of the endogenous mir-799 pre-miRNA via CRISPR/CAS9. Left Flanking: ATTTTCTATTTATTGGTATAAAATATGTTA, Right Flanking: AAGAAGTACACTTCATATGCTCCTAACAAT. sgRNA: GTGAACCCTGATAAAGCTAG. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott and Marcus L. Vargas Received: 02/21/22 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC177 Species: C. elegans Genotype: lin-4(umn84[lin-4p::SL1::EGL-13NLS::lox2272::mScarlet-I::cMycNLS::Lox511I::let-858 3'UTR::lox2722])/mIn1[dpy-10(e128) umnIs33] II. Description: umnIs33 [myo-2p::GFP + NeoR, II: 11755713 (intergenic)] II. Nuclear mScarlet-I was inserted in place of the endogenous lin-4 pre-miRNA via CRISPR/CAS9. Heterozygotes are wild-type mScarlet+ GFP+, and segregate wild-type mScarlet+ GFP+, Lin-4 mScarlet+ non-GFP (umn84 homozygotes), and Dpy non-mScarlet GFP+ (mIn1 homozygotes). Maintain by picking wild-type mScarlet+ GFP+. Left Flanking: AGAGTTTTGGTTGGTTTATGAGTTT, Right Flanking: CCAGGACGGTTTGAGCAGATCtttt. sgRNA: TGAGGTCTCAGGGAACAGGC. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Marcus L. Vargas Received: 02/14/22 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC179 Species: C. elegans Genotype: mir-82(umn86[mir-82p+SL1::EGL13NLS::lox2272]) X. Description: Deletion of mir-82 pre-miRNA via CRISPR/CAS9 and CRE/lox. Left Flanking: TATCATTCTCTCTACTACTAGTGAACTCAT, Right Flanking: TTATCAAGAAAATTCAAGAAAATTCAAAAG. sgRNA: CTGTAGATCACAGAGAAAAC. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott and Marcus L. Vargas Received: 02/23/22 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC18 Species: Caenorhabditis elegans Genotype: umnIs7 III. Description: umnIs7 [myo-2p::GFP + NeoR, III:9421936 (intergenic)] III. Derived by insertion of myo-2p::GFP transgene into parental strain N2 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Julie Knott Received: 05/10/16 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC180 Species: C. elegans Genotype: mir-82(umn87[mir-82p+SL1::EGL13NLS::lox2272mScarlet-I::cMycNLS::let-858 3' UTR::lox2272]) X. Description: Nuclear mScarlet-I was inserted in place of the endogenous mir-82 pre-miRNA via CRISPR/CAS9. Left Flanking: TATCATTCTCTCTACTACTAGTGAACTCAT, Right Flanking: TTATCAAGAAAATTCAAGAAAATTCAAAAG. sgRNA: CTGTAGATCACAGAGAAAAC. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott and Marcus L. Vargas Received: 02/23/22 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC19 Species: Caenorhabditis elegans Genotype: eT1 III; eT1 [umnIs8] V. Description: umnIs8 [myo-2p::GFP + NeoR, III:9421936 (intergenic)] V. Derived by insertion of myo-2p::GFP transgene into eT1 balancer in parental strain CB873 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 05/10/16 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC194 Species: C. elegans Genotype: dpy-26(n199)/tmC5 [F36H1.3(tmIs1220)] IV. Description: Lethal/sterile dpy-26 allele balanced over tmC5 labelled with Venus. Heterozygotes are wild-type with somewhat dimmer Venus signal and segregate WT Venus(+) heterozygotes, Mec Unc Venus(+) tmC5 homozygotes, and non-Venus n199 homozygotes (variable morphology). Pick wild-type Venus(+) and check for proper segregation of progeny to maintain. Derived by crossing parental strains FX30140 with CB5101 (males). Mutagen: N/A Outcrossed: x0 Made by: Aric Daul Received: 02/24/23 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC2 Species: C. briggsae Genotype: C. briggsae wild isolate. Description: C. briggsae reference strain formerly known as PB420. Derived from C. briggsae Gujarat, the strain that later was named G16 and then AF16. PB420 was frozen (as C. briggsae Gujarat) 22 March 1991, thawed 15 June 2020 and sent to the CGC 1 July 2020. It may be considered ancestral to AF16 and was renamed to distinguish it from AF16 strains that have been maintained in laboratory cultures. Reference: Fodor A, et al. Nematologica 1983 92: 203-217. doi:10/1163.187529283X00456 Mutagen: Outcrossed: x0 Made by: András Fodor Received: 07/08/20 Wright State University ----------------------------------------------------------------------------- Strain: CGC20 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177)/eT1[umnIs9] V. Description: umnIs9 [myo-2p::GFP + NeoR, III:9421936 (intergenic)] V. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, Unc-36 GFP+ (eT1), dead eggs, and DpyUncs. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into eT1 balancer in parental strain BC2200 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 07/07/16 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC22 Species: Caenorhabditis elegans Genotype: umnIs11 V. Description: umnIs11 [myo-2p::GFP + NeoR, V:1005689 (intergenic)] V. Derived by insertion of myo-2p::GFP transgene into parental strain N2 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Julie Knott Received: 08/12/16 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC23 Species: Caenorhabditis elegans Genotype: dpy-18(e364)/eT1 [umnIs12] III; unc-46(e177)/eT1 V. Description: umnIs12 [myo-2p::GFP + NeoR, V: 1005689 (intergenic)] III. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, Unc-36 GFP+ (eT1), dead eggs, and DpyUncs. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into eT1 balancer in parental strain BC2200 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Mindy Moseley Received: 09/21/16 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC24 Species: Caenorhabditis elegans Genotype: umnIs13 X. Description: umnIs13 [myo-2p::GFP + NeoR, X: 6745526 (intergenic)] X. Derived by insertion of myo-2p::GFP transgene into parental strain N2 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Julie Knott Received: 09/21/16 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC25 Species: Caenorhabditis elegans Genotype: hT2 [umnIs14] I; hT2 [bli-4(e937)] III. Description: umnIs14 [myo-2p::GFP + NeoR, III:9421936 (intergenic)] I. Homozygous viable. Derived by insertion of myo-2p::GFP transgene into hT2 balancer in parental strain KR1234 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 08/24/16 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC26 Species: Caenorhabditis elegans Genotype: dpy-5(e61)/hT2 [umnIs15] I; unc-36(e251)/hT2 [bli-4(e937) let-?(h661)] III. Description: umnIs15 [myo-2p::GFP + NeoR, III: 9421936 (intergenic)] I. Heterozygotes are WT GFP+ and segregate WT GFP+, DpyUnc, lethal GFP+ hT2 homozygotes (arrest stage unknown) and dead eggs (aneuploids). Will throw an occasional GFP+ Dpy non-Unc (similar events were observed in the parental hT2 strain). Pick WT GFP+ and check for correct segregation of progeny to maintain. Derived by insertion of myo-2p::GFP transgene into hT2 balancer in parental strain KR2467 using CRISPR/Cas9. [NOTE: 3/1995: Apparently the lethal mutation is closely linked but not within the balanced region of hT2. It can occasionally recombine away so that the strain will segregate Bli-4 hT2 homozygotes. (Mark Edgley)] Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 01/18/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC27 Species: Caenorhabditis elegans Genotype: umnIs16 X. Description: umnIs16 [myo-2p::GFP + NeoR, X:15420938 (intergenic)] X. Derived by insertion of myo-2p::GFP transgene into parental strain N2 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Julie Knott Received: 11/01/16 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC28 Species: Caenorhabditis elegans Genotype: +/szT1 [lon-2(e678) umnIs17] I; dpy-8(e1321) unc-3(e151)/szT1 X. Description: umnIs17 [myo-2p::GFP + NeoR, X: 6745526 (intergenic)] I. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, DpyUnc non-GFP, dead eggs and GFP+ Lon males. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into szT1 balancer in parental strain AF1 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 08/24/16 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC29 Species: Caenorhabditis elegans Genotype: unc-13(e51)/hT1 [umnIs18] I; dpy-11(e224)/hT1 [unc-42(e270)] V. Description: umnIs18 [myo-2p::GFP + NeoR, V: 1005689 (intergenic)] I. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, Dpy Unc, arrested hT1 homozygotes(GFP+), and dead eggs. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into hT1 balancer in parental strain KR1037 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Mindy Moseley Received: 09/19/16 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC3 Species: Caenorhabditis tropicalis Genotype: C. tropicalis wild isolate. Description: C. tropicalis reference strain formerly known as NIC58. Culture at 20°C or above. Mutagen: Outcrossed: x0 Made by: Erik Andersen Received: 02/12/25 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: CGC30 Species: Caenorhabditis elegans Genotype: unc-30(e191) dpy-4(e1166) IV; yDp1 [umnIs19] (IV;V;f). Description: umnIs19 [myo-2p::GFP + NeoR, V: 1005689 (intergenic)]. Animals with the Dup are wild-type GFP+; animals that have lost the Dup are Dpy Unc GFP-. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into yDp1 duplication in parental strain TY156 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Mindy Moseley Received: 09/19/16 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC31 Species: Caenorhabditis elegans Genotype: umnIs20 III. Description: umnIs20 [myo-2p::GFP + NeoR, III:518034 (intergenic)] III. Derived by insertion of myo-2p::GFP transgene into parental strain N2 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Julie Knott Received: 12/12/16 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC32 Species: Caenorhabditis elegans Genotype: sC1(s2023) [dpy-1(s2170) umnIs21] III. Description: umnIs21 [myo-2p::GFP + NeoR, III: 518034 (intergenic)]. Dpy GFP+. Derived by insertion of myo-2p::GFP transgene into sC1 balancer in parental strain BC4279 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Mindy Moseley Received: 09/28/16 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC33 Species: Caenorhabditis elegans Genotype: unc-5(e53)/nT1 [umnIs22] IV; dpy-11(e224)/nT1 V. Description: umnIs22 [myo-2p::GFP + NeoR, V: 1005689 (intergenic)] IV. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, DpyUnc, Vul GFP+ (nT1) and dead eggs. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into nT1 balancer in parental strain MT1000 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Mindy Moseley Received: 09/27/16 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC34 Species: Caenorhabditis elegans Genotype: eT1 [umnIs12] III; eT1 V. Description: umnIs12 [myo-2p::GFP + NeoR, V: 1005689 (intergenic)] III. Derived by insertion of myo-2p::GFP transgene into eT1 balancer in parental strain BC2200 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Mindy Moseley Received: 09/29/16 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC35 Species: Caenorhabditis elegans Genotype: +/szT1 [lon-2(e678) umnIs24] I; dpy-8(e1321) unc-3(e151)/szT1 X. Description: umnIs24 [myo-2p::GFP + NeoR, X: 15420938 (intergenic)] I. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, DpyUnc non-GFP, dead eggs and GFP+ Lon males. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into szT1 balancer in parental strain AF1 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 01/18/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC36 Species: Caenorhabditis elegans Genotype: mnDp1 [umnIs25] (X;V)/+ V; unc-3(e151) X. Description: umnIs25 [myo-2p::GFP + NeoR, X: 15420938 (intergenic)]. Pick wild-type GFP+ to maintain. Segregates lethals: homozygous Dp/Dp are lethal. Derived by insertion of myo-2p::GFP transgene into mnDp1 duplication in parental strain SP219 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Mindy Moseley Received: 09/29/16 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC37 Species: Caenorhabditis elegans Genotype: unc-3(e151) X; mnDp3 [umnIs26] (X;f). Description: umnIs26 [myo-2p::GFP + NeoR, X: 15420938 (intergenic)]. Pick wild-type GFP+ to maintain. Segregates wild-type GFP+ and Unc GFP-. Derived by insertion of myo-2p::GFP transgene into mnDp3 duplication in parental strain SP123 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Mindy Moseley Received: 11/22/16 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC38 Species: Caenorhabditis elegans Genotype: umnIs27 III. Description: umnIs27 [myo-2p::GFP + NeoR, III: 8856215 (intergenic)] III. Derived by insertion of myo-2p::GFP transgene into parental strain N2 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Julie Knott Received: 02/02/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC39 Species: Caenorhabditis elegans Genotype: unc-5(e53)/nT1 IV; dpy-11(e224)/nT1 [umnIs28] V. Description: umnIs28 [myo-2p::GFP + NeoR, IV: 12457861 (intergenic)] V. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, DpyUnc, Vul GFP+ (nT1) and dead eggs. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into nT1 balancer in parental strain MT1000 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Mindy Moseley Received: 01/18/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC4 Species: Caenorhabditis elegans Genotype: umnTi1 III. Description: umnTi1 [eft-3p::GFP + unc-119(+)]. Integration site: (III:-4.25 cM/ nt 3,771,500). Might still contain unc-119(ed3) in background. Mutagen: MosSCI Outcrossed: x Made by: Britt Werre Received: 04/03/15 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC40 Species: Caenorhabditis elegans Genotype: +/szT1 [lon-2(e678) umnIs29] I; dpy-8(e1321) unc-3(e151)/szT1 X. Description: umnIs29 [myo-2p::mKate2 + NeoR, X: 6745526 (intergenic)] I. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc non-GFP, dead eggs and mKate2+ Lon males. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate2 transgene into szT1 balancer in parental strain AF1 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 02/08/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC41 Species: Caenorhabditis elegans Genotype: +/szT1 [lon-2(e678) umnIs30 umnIs24] I; dpy-8(e1321) unc-3(e151)/szT1 X. Description: umnIs30 [myo-2p::mKate2 + NeoR, X: 6745526 (intergenic)] I. umnIs24 [myo-2p::GFP + NeoR, X: 15420938 (intergenic)] I. Carries szT1 balancer with mKate2 tag on left arm and GFP tag on right arm. Heterozygotes are wild-type GFP+ & mKate2+, and segregate wild-type GFP+ & mKate2+, DpyUnc non-GFP & non-mKate2, dead eggs and GFP+ & mKate2+ Lon males. Maintain by picking wild-type with GFP+ & mKate2+. Derived by insertion of myo-2p::mKate transgene into szT1 balancer in parental strain CGC35 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 02/28/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC42 Species: Caenorhabditis elegans Genotype: mnDp10 [umnIs31] (X;I); unc-3(e151) X. Description: umnIs31 [myo-2p::GFP + NeoR, X: 15420938 (intergenic)] I. Segregates mostly wild-type GFP+ and occasional Unc GFP-. Maintain by picking WT GFP+. Derived by insertion of myo-2p::GFP transgene into mnDp10 duplication in parental strain SP117 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Mindy Moseley Received: 01/24/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC43 Species: Caenorhabditis elegans Genotype: unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444) umnIs32] II. Description: umnIs32 [myo-2p::GFP + NeoR, II: 11755713 (intergenic)] II. Hets are WT GFP+ and segregate WT GFP+, Unc-4 (GFP-) and paralysed DpyUnc GFP+ (mnC1 homozygotes). Maintain by picking WT GFP+. Derived by insertion of myo-2p::GFP transgene into mnC1 balancer in parental strain SP127 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Mindy Moseley Received: 01/18/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC44 Species: Caenorhabditis elegans Genotype: unc-4(e120)/mIn1 [dpy-10(e128) umnIs33] II. Description: umnIs33 [myo-2p::GFP + NeoR, II: 11755713 (intergenic)] II. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, Unc-4 non-GFP, and Dpy GFP+ mIn1 homozygotes. Maintain by picking wild-type GFP+ and check for correct segregation of progeny to maintain. Derived by insertion of myo-2p::GFP transgene into mIn1 balancer in parental strain DR1785 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Mindy Moseley Received: 02/08/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC45 Species: Caenorhabditis elegans Genotype: unc-4(e120)/mT1 [umnIs34] II; mT1 [dpy-10(e128)]/dpy-17(e164) III. Description: umnIs34 [myo-2p::GFP + NeoR, III: 8856215 (intergenic)] II. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, DpyUnc non-GFP, sterile Dpy GFP+ mT1 homozygotes, and large numbers of arrested aneuploid embryos. Maintain by picking wild-type GFP+ and check for correct segregation of progeny to maintain. Derived by insertion of myo-2p::GFP transgene into mT1 balancer in parental strain DR1832 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 02/08/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC46 Species: Caenorhabditis elegans Genotype: +/szT1 [lon-2(e678)] I; dpy-8(e1321) unc-3(e151)/szT1 [umnIs35] X. Description: umnIs35 [myo-2p::GFP + NeoR, I: 6284001 (intergenic)] X. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, DpyUnc non-GFP, dead eggs and GFP+ Lon males. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into szT1 balancer in parental strain AF1 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 02/28/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC47 Species: Caenorhabditis elegans Genotype: hT2 I; hT2 [bli-4(e937) umnIs36] III. Description: umnIs36 [myo-2p::mKate2 + NeoR, I: 6284001 (intergenic)] III. Homozygous-viable translocation marked with bli-4 and mKate2. Derived by insertion of myo-2p::mKate2 transgene into hT2 balancer in parental strain KR1234 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 03/28/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC48 Species: Caenorhabditis elegans Genotype: unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444) umnIs37] II. Description: umnIs37 [myo-2p::mKate2 + NeoR, II: 11755713 (intergenic)] II. Hets are WT mKate2+ and segregate WT mKate2+, Unc-4 (no red fluorescence) and paralysed DpyUnc mKate2+ (mnC1). Maintain by picking WT mKate2+. Derived by insertion of myo-2p::mKate2 transgene into parental strain SP127 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 04/02/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC49 Species: Caenorhabditis elegans Genotype: hT2 I; hT2 [bli-4(e937) umnIs38] III. Description: umnIs38 [myo-2p::GFP + NeoR, I: 6284001 (intergenic)] III. Homozygous-viable translocation marked with bli-4 and GFP. Derived by insertion of myo-2p::GFP transgene into hT2 balancer in parental strain KR1234 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 04/02/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC5 Species: Caenorhabditis elegans Genotype: umnTi2 III. Description: umnTi2 [eft-3p::GFP + unc-119(+)]. Integration site: (III:+13.25 cM/nt 11,816,400). Might still contain unc-119(ed3) in background. Mutagen: MosSCI Outcrossed: x Made by: Britt Werre Received: 04/03/15 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC50 Species: Caenorhabditis elegans Genotype: +/szT1 [lon-2(e678) umnIs39] I; dpy-8(e1321) unc-3(e151)/szT1 [umnIs40] X. Description: umnIs39 [myo-2p::mKate2 + NeoR, X: 6745526 (intergenic)] I. umnIs40 [myo-2p::GFP + NeoR, I: 6284001 (intergenic)] X. Heterozygotes are wild-type GFP+ & mKate2+, and segregate wild-type GFP+ & mKate2+, DpyUnc non-GFP & non-mKate2, dead eggs, and GFP+ & mKate2+ Lon males. Maintain by picking wild-type with GFP+ & mKate2+. Derived by simultaneous insertion of independent myo-2p::mKate2 and myo-2p::GFP transgenes into each portion of the szT1 balancer in parental strain AF1 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 04/19/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC51 Species: Caenorhabditis elegans Genotype: sC1(s2023) [dpy-1(s2170) umnIs41] III. Description: umnIs41 [myo-2p::mKate2 + NeoR, III: 518034 (intergenic)] III. Dpy mKate2+. Derived by insertion of myo-2p::mKate2 transgene into parental strain BC4279 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 04/19/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC52 Species: C. elegans Genotype: dpy-5(e61)/hT2 I; unc-36(e251)/hT2 [bli-4(e937) let-?(h661) umnIs42] III. Description: umnIs42 [myo-2p::mKate2 + NeoR, I: 6284001 (intergenic)] III. Heterozygotes are WT mKate2+ and segregate WT mKate2+, DpyUnc, lethal mKate2+ hT2 homozygotes (arrest stage unknown) and dead eggs (aneuploids). Will throw an occasional mKate2+ Dpy non-Unc (similar events were observed in the parental hT2 strain). Pick WT mKate2+ and check for correct segregation of progeny to maintain. Derived by insertion of myo-2p::mKate2 transgene into hT2 balancer in parental strain KR2467 using CRISPR/Cas9. [NOTE: 3/1995: Apparently the lethal mutation is closely linked but not within the balanced region of hT2. It can occasionally recombine away so that the strain will segregate Bli-4 hT2 homozygotes. (Mark Edgley)] Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott Received: 07/26/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC53 Species: C. elegans Genotype: unc-4(e120)/mIn1 [dpy-10(e128) umnIs43] II. Description: umnIs43 [myo-2p::mKate2 + NeoR, II: 11755713 (intergenic)] II. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2, Unc-4 non-mKate2, and Dpy mKate2+ mIn1 homozygotes. Maintain by picking wild-type mKate2+ and check for correct segregation of progeny to maintain. Derived by insertion of myo-2p::mKate2 transgene into mIn1 balancer in parental strain DR1785 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott Received: 06/07/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC54 Species: C. elegans Genotype: umnIs44 II. Description: umnIs44 [myo-2p::mKate2 + NeoR, II: 11755713 (intergenic)] II. Derived by insertion of myo-2p::mKate2 transgene into parental strain N2 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Julie Knott Received: 08/16/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC55 Species: Caenorhabditis elegans Genotype: eT1 [umnIs45] III; eT1 V. Description: umnIs45 [myo-2p::mKate2 + NeoR, V: 1005689 (intergenic)] III. Derived by insertion of myo-2p::mKate2 transgene into eT1 balancer in parental strain CB873 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott Received: 06/30/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC57 Species: C. elegans Genotype: umnIs47 III. Description: umnIs47 [myo-2p::mKate2 + NeoR, III: 9421936 (intergenic)] III. Derived by insertion of myo-2p::mKate2 transgene into parental strain N2 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Julie Knott Received: 09/14/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC58 Species: C. elegans Genotype: C54E10.3(umn2[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) V. Description: Homozygous viable. Deletion of 745 bp with Calarco/Colaiacovo selection cassette conferring myo-2::GFP and G418 resistance inserted at break in parental strain N2. Left flanking Sequence: tgtacccccgatgggattcgaacctgtggc ; Right flanking sequence: gggtatgcaaaatgaccgcgttttctgtga. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: RG KO Group Received: 09/18/17 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CGC59 Species: C.elegans Genotype: gnrr-7(umn3[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) X. Description: Homozygous viable. Deletion of 1004 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Left flanking Sequence: ttgttctggtttaaagccgcaaagtcttgg ; Right flanking sequence: agggtaccatcaagcaatggcattctggtt. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: RG KO Group Received: 09/18/17 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CGC6 Species: Caenorhabditis elegans Genotype: umnTi3 I. Description: umnTi3 [eft-3p::GFP + unc-119(+)]. Integration site: (I:+30 cM/nt 15,065,881). Might still contain unc-119(ed3) in background. Mutagen: MosSCI Outcrossed: x2 Made by: Britt Werre Received: 04/03/15 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC60 Species: C. elegans Genotype: dpy-18(e364)/eT1 III; unc-46(e177)/eT1[umnIs46] V. Description: umnIs46 [myo-2p::mKate2 + NeoR, III:9421936 (intergenic)] V. Heterozygotes are wild-type mKate+, and segregate wild-type mKate2+, Unc-36 mKate+ (eT1), dead eggs, and DpyUncs. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate2 transgene into eT1 balancer in parental strain BC2200 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott Received: 09/22/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC61 Species: C. elegans Genotype: F36D4.4(umn4[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) V. Description: Homozygous viable. Deletion of 917 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Left flanking Sequence: CATGTACTCCCCTATATCTTCCAAACATTC ; Right flanking sequence: TGGACATCTTGGAGCACTTTCTGTGATTCT. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: RG KO Group Received: 09/26/17 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CGC62 Species: C.elegans Genotype: umnIs48 V. Description: umnIs48 [myo-2p::mKate2 + NeoR, V:1005689 (intergenic)] V. Derived by insertion of myo-2p::mKate2 transgene into parental strain N2 using CRISPR/Cas9. Mutagen: Outcrossed: x2 Made by: Julie Knott Received: 11/02/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC63 Species: C. elegans Genotype: unc-5(e53)/nT1 [umnIs49] IV; dpy-11(e224)/nT1 V. Description: umnIs49 [myo-2p::mKate2 + NeoR, V: 1005689 (intergenic)] IV. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc, Vul mKate2+ (nT1) and dead eggs. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate2 transgene into nT1 balancer in parental strain MT1000 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott Received: 10/12/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC64 Species: C. elegans Genotype: unc-30(e191) dpy-4(e1166) IV; yDp1 [umnIs50] (IV;V;f). Description: umnIs50 [myo-2p::mKate2 + NeoR, V: 1005689 (intergenic)]. Animals with the Dup are wild-type mKate2+; animals that have lost the Dup are Dpy Unc mKate2-. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate2 transgene into yDp1 duplication in parental strain TY156 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott Received: 10/10/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC65 Species: C. elegans Genotype: mT1/unc-4(e120) II; mT1 [dpy-10(e128) umnIs51]/dpy-17(e164) III. Description: umnIs51 [myo-2p::mKate2 + NeoR, II: 11755713 (intergenic)] III. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc non-mKate2, sterile Dpy mKate2+ mT1 homozygotes, and large numbers of arrested aneuploid embryos. Maintain by picking wild-type mKate2+ and check for correct segregation of progeny to maintain. Derived by insertion of myo-2p::mKate2 transgene into mT1 balancer in parental strain DR1832 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott Received: 10/10/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC66 Species: C. elegans Genotype: unc-4(e120)/mT1 [umnIs52] II; mT1 [dpy-10(e128)]/dpy-17(e164) III. Description: umnIs52 [myo-2p::mKate2 + NeoR, III: 8856215 (intergenic)] II. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc non-mKate2, sterile Dpy mKate2+ mT1 homozygotes, and large numbers of arrested aneuploid embryos. Maintain by picking wild-type mKate2+ and check for correct segregation of progeny to maintain. Derived by insertion of myo-2p::mKate2 transgene into mT1 balancer in parental strain DR1832 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott Received: 12/14/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC67 Species: C. elegans Genotype: umnIs53 V. Description: umnIs53 [myo-2p::GFP + NeoR, V:18120303 (intergenic)] V. Derived by insertion of myo-2p::GFP transgene into parental strain N2 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Julie Knott Received: 12/14/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC68 Species: C. elegans Genotype: mT1/unc-4(e120) II; mT1 [dpy-10(e128) umnIs54]/dpy-17(e164) III. Description: umnIs54 [myo-2p::GFP + NeoR, II: 11755713 (intergenic)] III. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, DpyUnc non-mGFP, sterile Dpy GFP+ mT1 homozygotes, and large numbers of arrested aneuploid embryos. Maintain by picking wild-type GFP+ and check for correct segregation of progeny to maintain. Derived by insertion of myo-2p::GFP transgene into mT1 balancer in parental strain DR1832 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott Received: 12/14/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC69 Species: C. elegans Genotype: dpy-18(e364)/eT1 [umnIs55] III; unc-46(e177)/eT1 V. Description: umnIs55 [myo-2p::mKate2 + NeoR, V:1005689 (intergenic)] III. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, Unc-36 mKate+(eT1), dead eggs, and DpyUncs. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate2 transgene into eT1 balancer in parental strain BC2200 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott Received: 12/14/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC7 Species: Caenorhabditis elegans Genotype: umnTi4 I. Description: umnTi4 [eft-3p::GFP + unc-119(+)]. Integration site: (I:+1.58 cM/nt 6,966,807). Might still contain unc-119(ed3) in background. Mutagen: MosSCI Outcrossed: x2 Made by: Britt Werre Received: 04/03/15 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC70 Species: C.elegans Genotype: eT1 III; eT1 [umnIs56] V. Description: umnIs56 [myo-2p::mKate2 + NeoR, III: 9421936 (intergenic)] V. Derived by insertion of myo-2p::mKate2 transgene into eT1 balancer in parental strain CB873 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott Received: 12/14/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC71 Species: C. elegans Genotype: unc-5(e53)/nT1 IV; dpy-11(e224)/nT1 [umnIs57] V. Description: umnIs57 [myo-2p::mKate2 + NeoR, IV: 12457861 (intergenic)] V. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc, Vul mKate2+ (nT1) and dead eggs. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate2 transgene into nT1 balancer in parental strain MT1000 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott Received: 12/14/17 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC72 Species: C. elegans Genotype: npr-23(umn5[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) I. Description: Homozygous viable. Deletion of 280 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Left flanking Sequence: AAGGCGTCATCTGGAGAGAAGAACGAAgtg ; Right flanking sequence: CGGACACTTGTGCTTCACCAACTTGATCGC. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: RG KO Group Received: 12/14/17 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CGC73 Species: C. elegans Genotype: npr-28(umn6[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) X. Description: Homozygous viable. Deletion of 842 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Left flanking Sequence: TATTTGGTATCATTTTTCTAGCCGACTTTC ; Right flanking sequence: TGGACTTGTTTTCACTCATCCCTGTACCGA. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: RG KO Group Received: 12/14/17 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CGC75 Species: C. elegans Genotype: unc-13(e51)/hT1 [umnIs58] I; dpy-11(e224)/hT1 [unc-42(e270)] V. Description: umnIs58 [myo-2p::mKate2 + NeoR, V: 1005689 (intergenic)] V. Pick wild-type mKate2+ to maintain. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc, arrested hT1 homozygotes (mKate2+), and dead eggs. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate2 transgene into hT1 balancer in parental strain KR1037 using CRISPR/Cas9. Mutagen: Outcrossed: x0 Made by: Julie Knott Received: 01/05/18 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC76 Species: C. elegans Genotype: umnIs59 I. Description: umnIs59 [myo-2p::mKate2 + NeoR, I:6284001(intergenic)] I. Derived by insertion of myo-2p::mKate2 transgene into parental strain N2 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Julie Knott Received: 01/01/18 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC77 Species: C. elegans Genotype: hT2 [umnIs60] I; hT2 [bli-4(e937)] III. Description: umnIs60 [myo-2p::mKate2 + NeoR, III: 9421936 (intergenic)] I. Homozygous-viable translocation marked with bli-4 and mKate2. Derived by insertion of myo-2p::mKate2 transgene into hT2 balancer in parental strain KR1234 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott Received: 01/01/18 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC78 Species: C. elegans Genotype: C04C3.6(umn8[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) IV. Description: Homozygous viable. Deletion of 1123 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Left flanking Sequence: aaaaatcaactatttttaatgaaaatttca ; Right flanking sequence: TGGTCACTTTACCTGCGTTGATATTCATGT. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: RG KO Group Received: 01/08/18 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CGC79 Species: Caenorhabditis elegans Genotype: +/szT1 [lon-2(e678) umnIs61] I; dpy-8(e1321) unc-3(e151)/szT1 X. Description: umnIs61 [myo-2p::mKate2 + NeoR, X: 15420938 (intergenic)] I. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc non-mKate2, dead eggs and mKate2+ Lon males. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate2 transgene into szT1 balancer in parental strain AF1 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott Received: 01/16/18 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC8 Species: Caenorhabditis elegans Genotype: umnTi5 IV. Description: umnTi5 [eft-3p::GFP + unc-119(+)]. Integration site: (IV:+8.48 cM/nt 13,215,045). Might still contain unc-119(ed3) in background. Mutagen: MosSCI Outcrossed: x Made by: Britt Werre Received: 04/03/15 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC80 Species: C. elegans Genotype: umnIs62 III. Description: umnIs62 [myo-2p::mKate2 + NeoR, III:8856215 (intergenic)] III. Derived by insertion of myo-2p::mKate2 transgene into parental strain N2 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Julie Knott Received: 02/09/18 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC81 Species: C. elegans Genotype: C09F12.3(umn9[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) X. Description: Homozgous viable. Deletion of 1171 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Left flanking Sequence: acaatttacattaacttttcattatttcag ; Right flanking sequence: tggatgtgcattttttcgctgctcactctt. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: RG KO Group Received: 02/14/18 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CGC82 Species: C. elegans Genotype: umnIs63 II. Description: umnIs63 [myo-2p::GFP + NeoR, II:11755713 (intergenic)] II. Derived by insertion of myo-2p::GFP transgene into parental strain N2 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Julie Knott Received: 03/01/18 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC83 Species: C. elegans Genotype: tmIn8 [umnIs64] II. Description: umnIs64 [myo-2p::GFP + NeoR, II:12833878 (intergenic)] II. tmIn8 is a CRISPR/Cas9-induced inversion between F13D12.6 and cup-14 in LG II covering region (Mb) 2.1 (11.7..13.9). Derived by insertion of myo-2p::GFP transgene into parental strain FX19134 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Emily Lorenz-Mueller Received: 03/20/18 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC84 Species: C.elegans Genotype: umnIs65 V. Description: umnIs65 [myo-2p::mKate2 + NeoR, V:4308261 (intergenic)] V. Derived by insertion of myo-2p::mKate2 transgene into parental strain N2 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Emily Lorenz-Mueller Received: 03/22/18 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC85 Species: C. elegans Genotype: umnIs66 II. Description: umnIs66 [myo-2p::GFP + NeoR, II:12833878 (intergenic)] II. Derived by insertion of myo-2p::GFP transgene into parental strain N2 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Emily Lorenz-Mueller Received: 03/22/18 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC86 Species: C. elegans Genotype: dpy-5(e61)/hT2 I; unc-36(e251)/hT2 [bli-4(e937) let-?(h661) umnIs67] III. Description: umnIs67 [myo-2p::GFP + NeoR, I: 6284001 (intergenic)] III. Heterozygotes are WT GFP+ and segregate WT GFP+, DpyUnc, lethal GFP+ hT2 homozygotes (arrest stage unknown) and dead eggs (aneuploids). Will throw an occasional GFP+ Dpy non-Unc (similar events were observed in the parental hT2 strain). Pick WT GFP+ and check for correct segregation of progeny to maintain. Derived by insertion of myo-2p::GFP transgene into hT2 balancer in parental strain KR2467 using CRISPR/Cas9. [NOTE: 3/1995: Apparently the lethal mutation is closely linked but not within the balanced region of hT2. It can occasionally recombine away so that the strain will segregate Bli-4 hT2 homozygotes. (Mark Edgley)] Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott Received: 03/28/18 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC87 Species: C. elegans Genotype: tmIn54 [umnIs69] V. Description: umnIs69 [myo-2p::GFP + NeoR, V:4308261(intergenic)] V. Break points: In(srbc-66 T10H9.8) V. Covered region (Mb) 3.1 (3.5..6.7). Derived by insertion of myo-2p::GFP transgene into parental strain FX19702 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Emily Lorenz-Mueller Received: 05/01/18 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC88 Species: C. elegans Genotype: tmIn26 [umnIs70] X. Description: umnIs70 [myo-2p::GFP + NeoR, X:6745526(intergenic)] X. tmIn26 homozygotes are Lon and Mec. Break points: In(lon-2 mec-10) X. Covered region (Mb) 3.7 (4.7..8.5) Lon Mec. Derived by insertion of myo-2p::GFP transgene into parental strain FX19171 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Emily Lorenz-Mueller Received: 05/01/18 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC89 Species: C. elegans Genotype: tmIn58 [umnIs68] I; lig-4(tm750) III. Description: umnIs68 [myo-2p::GFP + NeoR, I:6284001(intergenic)] I. Break points: In(gsp-3 sre-23) I. Covered region (Mb) 3.5 (4.7..8.3). Derived by insertion of myo-2p::GFP transgene into parental strain FX19704 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Emily Lorenz-Mueller Received: 04/10/18 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC9 Species: Caenorhabditis elegans Genotype: umnTi6 III. Description: umnTi6 [eft-3p::GFP + unc-119(+)]. Integration site: (III:-1.43 cM/nt 5,935,821). Might still contain unc-119(ed3) in background. Mutagen: MosSCI Outcrossed: x Made by: Britt Werre Received: 04/03/15 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC91 Species: C. elegans Genotype: umnIs72 I. Description: umnIs72 [myo-2p::GFP + NeoR, I:6284001 (intergenic)] I. Derived by insertion of myo-2p::GFP transgene into parental strain N2 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Julie Knott Received: 05/29/18 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC92 Species: C.elegans Genotype: dpy-5(e61)/hT2 [umnIs73] I; unc-36(e251)/hT2 [bli-4(e937) let-?(h661)] III. Description: umnIs73 [myo-2p::mKate2 + NeoR, III: 9421936 (intergenic)] I. Heterozygotes are WT mKate2+ and segregate WT mKate2+, DpyUnc, lethal mKate2+ hT2 homozygotes (arrest stage unknown) and dead eggs (aneuploids). Will throw an occasional mKate+ Dpy non-Unc (similar events were observed in the parental hT2 strain). Pick WT mKate2+ and check for correct segregation of progeny to maintain. Derived by insertion of myo-2p::mKate2 transgene into hT2 balancer in parental strain KR2467 using CRISPR/Cas9. [NOTE: 3/1995: Apparently the lethal mutation is closely linked but not within the balanced region of hT2. It can occasionally recombine away so that the strain will segregate Bli-4 hT2 homozygotes. (Mark Edgley)] Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott Received: 06/11/18 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC93 Species: C. elegans Genotype: umnIs74 X. Description: umnIs74 [myo-2p::mKate2 + NeoR, X: 15420938 (intergenic)] X. Derived by insertion of myo-2p::mKate2 transgene into parental strain N2 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Julie Knott Received: 07/20/18 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC94 Species: C. elegans Genotype: hIn1 [umnIs75] I. Description: umnIs75 [myo-2p::GFP + NeoR, I: 12541645 (intergenic)] I. Superficially wild-type. Crossover suppressor for LGI right. Inversion includes unc-75 and unc-54. Derived by insertion of myo-2p::GFP transgene into hIn1 inversion in parental strain KR1949 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott Received: 10/10/18 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC96 Species: C. elegans Genotype: umnIs76 V. Description: umnIs76 [myo-2p::GFP + NeoR, V:4308261 (intergenic)] V. Derived by insertion of myo-2p::GFP transgene into parental strain N2 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Julie Knott Received: 11/07/18 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CGC97 Species: C. elegans Genotype: +/szT1 [lon-2(e678)] I; dpy-8(e1321) unc-3(e151)/szT1 [umnIs77] X. Description: umnIs77 [myo-2p::mKate2 + NeoR, I: 6284001 (intergenic)] X. Heterozygotes are wild-type mKate2+, and segregate wild-type mKate2+, DpyUnc non-mKate2, dead eggs and mKate2+ Lon males. Maintain by picking wild-type mKate2+. Derived by insertion of myo-2p::mKate2 transgene into szT1 balancer in parental strain AF1 using CRISPR/Cas9. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Julie Knott Received: 12/18/18 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: CH116 Species: Caenorhabditis elegans Genotype: hsb-1(cg116) IV. Description: Viable and fertile. Deletion of 664 bp, molecular null. Mutagen: UV/TMP Outcrossed: x6 Made by: S. Satyal Received: 03/26/99 Northwestern University Medical School, Chicago, IL ----------------------------------------------------------------------------- Strain: CH1179 Species: Caenorhabditis elegans Genotype: unc-36(e251) emb-9(g23cg46)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, Dpy Steriles and 3-fold lethals. cg46 is a 497 bp deletion that removes the last 22 nucleotides of intron 9 and 475 nucleotides of exon 10; Mutagen: EMS Outcrossed: x4 Made by: Malini Gupta Received: 12/15/97 Northwestern University Medical School, Chicago, IL ----------------------------------------------------------------------------- Strain: CH118 Species: Caenorhabditis elegans Genotype: nid-1(cg118) V. Description: Parental strain was CH1416 mut-2(r459) I; nid-1(ev608) V. Tc1 excision deletion of nid-1 was identified. In frame deletion, removes amino acids Thr53-Gln693 of NID-1. Homozygous viable and fertile, slightly reduced fertility. mut-2 was removed by outcrossing. nid-1=F54F3.1. Received new stock 1/2004 from Jim Kramer. Mutagen: Tc1 Outcrossed: x6 Made by: J. Kramer Received: 03/26/99 Northwestern University Medical School, Chicago, IL ----------------------------------------------------------------------------- Strain: CH1180 Species: Caenorhabditis elegans Genotype: unc-32(e189) emb-9(cg56)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, Dpy Steriles and Uncs which arrest in the L1 stage. Mutagen: EMS Outcrossed: x4 Made by: Malini Gupta Received: 12/15/97 Northwestern University Medical School, Chicago, IL ----------------------------------------------------------------------------- Strain: CH119 Species: Caenorhabditis elegans Genotype: nid-1(cg119) V. Description: Parental strain was CH1416 mut-2(r459) I; nid-1(ev608) V. Tc1 excision deletion of nid-1 was identified. Molecular null, deletion removes promoter region. Homozygous viable and fertile, fecundity reduced by approximately 30%. nid-1=F54F3.1. Mutagen: Tc1 Outcrossed: x6 Made by: J. Kramer Received: 03/26/99 Northwestern University Medical School, Chicago, IL ----------------------------------------------------------------------------- Strain: CH120 Species: Caenorhabditis elegans Genotype: cle-1(cg120) I. Description: Homozygous viable and fertile. Partially penetrant Egl and cell/axon guidance defects. Deletion of nucleotides 22756-24758 based on cosmid F39H11 sequence (Genbank AF164959). Results in loss of carboxyl NC1 domain from CLE-1. Mutagen: UV/TMP Outcrossed: x8 Made by: B. Ackley Received: 08/30/01 Northwestern University Medical School, Chicago, IL ----------------------------------------------------------------------------- Strain: CH121 Species: Caenorhabditis elegans Genotype: dgn-1(cg121)/dpy-6(e14) unc-115(mn481) X. Description: Heterozygotes are WT and segregate WT, DpyUncs, and Ste (and Gon) cg121 homozygotes. Mutagen: UV/TMP Outcrossed: x6 Made by: Jim Kramer Received: 06/15/06 Northwestern University Medical School, Chicago, IL ----------------------------------------------------------------------------- Strain: CH123 Species: Caenorhabditis elegans Genotype: dgn-2(ok209) X. Description: Animals appear wild type. Mutagen: UV/TMP Outcrossed: x8 Made by: Jim Kramer Received: 05/17/06 Northwestern University Medical School, Chicago, IL ----------------------------------------------------------------------------- Strain: CH1315 Species: Caenorhabditis elegans Genotype: zmp-1(cg115) III. Description: Superficially wild-type. 2366 bp deletion (965-3330 of U41266(EGAP1)) caused by imprecise excision of Tc1. Deletion can be detected by PCR with primers DSP4 (AATTAGTTGACGAGACAAGTCAGG) and B3 (AGTGAAGGCAGAATGTACTCC) --306 kb WT vs 1.2 kb mutant. Mutagen: Tc1 Excision Outcrossed: x7 Made by: James Kramer Received: 03/03/10 Northwestern University Medical School, Chicago, IL ----------------------------------------------------------------------------- Strain: CH1445 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; cgEx198. Description: cgEx198 [(pJC14) bli-1::GFP + unc-119(+)]. Pick non-Unc to maintain. Transgene rescues bli-1 phenoptype. GFP expression is detectable in late L4-adult. Mutagen: Outcrossed: x Made by: James Kramer Received: 11/03/11 Northwestern University Medical School, Chicago, IL ----------------------------------------------------------------------------- Strain: CH1869 Species: Caenorhabditis elegans Genotype: dgn-1(cg121) X; cgEx308. Description: cgEx308 [dgn-1(+) + dng-1p::GFP + rol-6(su1006)]. Rollers. Pick rollers to maintain. Segregates sterile non-rollers (dgn-1 homozygotes) and fertile rollers (dgn-1 rescued). Rollers can be used to produce cg121/o; cgEx308 males that can mate. Mutagen: Outcrossed: x Made by: Robert Johnson Received: 04/30/12 Northwestern University Medical School, Chicago, IL ----------------------------------------------------------------------------- Strain: CH1878 Species: Caenorhabditis elegans Genotype: dgn-2(ok209) dgn-3(tm1092) dgn-1(cg121) X; cgEx308. Description: cgEx308 [pJK600/dgn-1(+) + pJK602/dng-1p::GFP + rol-6(su1066)]. Rollers. Pick rollers to maintain. Segregates sterile non-rollers (dgn-2 dgn-3 dgn-1 homozygotes) and fertile rollers (dgn-1 rescued). Rollers can be used to produce cg121/o; cgEx308 males that can mate. Triple mutants resemble dgn-1 single mutants; no overt phenotype caused by dgn-2 dgn-3 mutations. Mutagen: Outcrossed: x Made by: James Kramer Received: 04/30/12 Northwestern University Medical School, Chicago, IL ----------------------------------------------------------------------------- Strain: CHS1001 Species: C. elegans Genotype: srh-99(yum1120) srh-100(yum1121) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1002 Species: C. elegans Genotype: srh-105(yum1122) II; srh-109(yum1123) srh-111(yum1124) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1003 Species: C. elegans Genotype: srh-105(yum1122) II; srh-104(yum1126) srh-102(yum1125) srh-109(yum1123) srh-111(yum1124) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1004 Species: C. elegans Genotype: srh-141(yum1127) srh-142(yum1128) srh-145(yum1129) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1005 Species: C. elegans Genotype: srh-146(yum1130) srh-147(yum1131) srh-148(yum1132) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1006 Species: C. elegans Genotype: srh-190(yum1133) srh-192(yum1134) srh-193(yum1135) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1007 Species: C. elegans Genotype: srh-203(yum1136) srh-204(yum1137) srh-206(yum1138) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1008 Species: C. elegans Genotype: srh-213(yum1140) srh-212(yum1139) srh-218(yum1141) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1009 Species: C. elegans Genotype: srh-297(yum1144) II; srh-295(yum1142) srh-296(yum1143) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1010 Species: C. elegans Genotype: str-112(yum1145) str-113(yum1146) str-114(yum1147) str-115(yum1148) str-116(yum1149) str-118(yum1150) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1011 Species: C. elegans Genotype: sri-1(yum1151) sri-2(yum1152) sri-4(yum1153) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1012 Species: C. elegans Genotype: srbc-61(yum1154) srbc-62(yum1155) srbc-63(yum1156) srbc-65(yum1157) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1013 Species: C. elegans Genotype: sro-1(yum1158) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1014 Species: C. elegans Genotype: sru-47(yum1163) I; sru-7(yum1159) IV; c45h4.18(yum1162) sru-44(yum1160) sru-45(yum1161) V; sru-48(yum1164) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1015 Species: C. elegans Genotype: gar-2(yum1166) III; gar-3(yum1167) V; gar-1(yum1165) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1016 Species: C. elegans Genotype: gbb-2(yum1169) IV; gbb-1(yum1168) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1017 Species: C. elegans Genotype: y40c5a.4(yum1171) IV; c47e8.3(yum1172) f57a8.4(yum1170) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1018 Species: C. elegans Genotype: b0034.5(yum1175) II; f56a11.4(yum1174) y37e11al.1(yum1173) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1019 Species: C. elegans Genotype: f16c3.1(yum1177) I; sphr-1(yum1178) h09f14.1(yum1176) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1020 Species: C. elegans Genotype: k03h6.1(yum1032) k03h6.5(yum1031) r13h7.2(yum1030) IV; t10e10.3(yum1033) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1021 Species: C. elegans Genotype: f59b2.13(yum1180) w10c4.1(yum1181) III; d1014.2(yum1182) f40a3.7(yum1179) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1022 Species: C. elegans Genotype: srg-2(yum1183) srg-5(yum1184) srg-6(yum1185) srg-7(yum1186) srg-8(yum1187) srg-9(yum1188) III. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1023 Species: C. elegans Genotype: srx-45(yum1190) III; srx-47(yum1192) srx-46(yum1191) srx-44(yum1189) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1024 Species: C. elegans Genotype: pdfr-1(yum1024) III; seb-2(yum1025) V; seb-3(yum1026) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1025 Species: C. elegans Genotype: frpr-10(yum1196) IV; frpr-9(yum1195) V; frpr-7(yum1193) frpr-8(yum1194) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1026 Species: C. elegans Genotype: frpr-19(yum1199) IV; frpr-13(yum1197) frpr-15(yum1198) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1027 Species: C. elegans Genotype: srj-18(yum1200) srj-19(yum1201) srj-20(yum1202) srj-22(yum1203) srj-23(yum1204) srj-24(yum1205) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1028 Species: C. elegans Genotype: srj-7(yum1206) srj-8(yum1207) srj-9(yum1208) srj-10(yum1209) srj-11(yum1210) srj-13(yum1211) srj-14(yum1212) srj-15(yum1213) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1029 Species: C. elegans Genotype: sru-35(yum1218) IV; sru-29(yum1215) sru-30(yum1216) sru-31(yum1217) sru-36(yum1219) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1030 Species: C. elegans Genotype: str-148(yum1223) II; str-141(yum1224) str-146(yum1222) str-145(yum1221) str-149(yum1225) str-151(yum1227) str-150(yum1226) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1031 Species: C. elegans Genotype: sri-28(yum1229) sri-29(yum1230) sri-30(yum1231) sri-31(yum1232) t10d4.14(yum1236) II; sri-24(yum1233) sri-25(yum1234) sri-26(yum1235) sri-27(yum1228) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1032 Species: C. elegans Genotype: srw-115(yum1237) srw-117(yum1238) srw-118(yum1239) srw-119(yum1240) srw-120(yum1241) srw-121(yum1242) srw-122(yum1243) srw-123(yum1244) srw-124(yum1245) srw-135(yum1246) srw-137(yum1247) srw-138(yum1248) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1033 Species: C. elegans Genotype: srh-127(yum1249) srh-211(yum1250) srh-212(yum1255) srh-213(yum1256) srh-214(yum1251) srh-215(yum1252) srh-216(yum1253) srh-217(yum1254) srh-218(yum1257) srh-219(yum1258) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1034 Species: C. elegans Genotype: srg-65(yum1267) srg-66(yum1268) srg-67(yum1269) srg-68(yum1270) V; srg-56(yum1259) srg-57(yum1260) srg-58(yum1261) srg-59(yum1262) srg-60(yum1263) srg-61(yum1264) srg-62(yum1265) V; srg-64(yum1266) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1035 Species: C. elegans Genotype: str-153(yum1271) IV; str-154(yum1280) V; str-155(yum1272) str-156(yum1275) str-158(yum1276) str-159(yum1277) IV; str-160(yum1278) V; str-161(yum1279) str-162(yum1273) str-168(yum1274) IV; str-267(yum1281) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1036 Species: C. elegans Genotype: srh-61(yum1282) II; srh-62(yum1283) V; srh-63(yum1284) IV; srh-67(yum1285) srh-68(yum1286) srh-69(yum1287) srh-70(yum1288) srh-71(yum1289) srh-72(yum1290) srh-73(yum1291) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1037 Species: C. elegans Genotype: srd-60(yum1292) srd-61(yum1294) srd-71(yum1296) srd-72(yum1297) srd-73(yum1293) srd-75(yum1295) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1038 Species: C. elegans Genotype: srt-1(yum1306) srt-2(yum1307) srt-3(yum1308) srt-4(yum1309) srt-5(yum1310) srt-6(yum1298) srt-7(yum1299) srt-8(yum1300) V; srt-11(yum1301) II; srt-12(yum1302) srt-13(yum1303) srt-14(yum1304) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1039 Species: C. elegans Genotype: sre-43(yum1311) sre-44(yum1312) sre-45(yum1313) sre-46(yum1314) sre-47(yum1315) sre-48(yum1316) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1040 Species: C. elegans Genotype: nmur-1(yum1317) X; nmur-2(yum1318) II; nmur-3(yum1319) X; nmur-4(yum1320) I. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1041 Species: C. elegans Genotype: frpr-3(yum1321) V; frpr-4(yum1322) frpr-16(yum1323) II; frpr-18(yum1324) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1042 Species: C. elegans Genotype: srh-89(yum1325) srh-92(yum1326) srh-95(yum1327) srh-97(yum1328) srh-98(yum1329) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1043 Species: C. elegans Genotype: srh-39(yum1330) II; srh-40(yum1331) III; srh-41(yum1332) srh-42(yum1333) srh-44(yum1334) II; srh-45(yum1335) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1044 Species: C. elegans Genotype: srh-220(yum1336) srh-222(yum1337) srh-223(yum1338) srh-227(yum1339) srh-228(yum1340) IV; srh-229(yum1341) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1045 Species: C. elegans Genotype: srh-49(yum1343) srh-50(yum1344) srh-51(yum1345) I; srh-48(yum1342) II; srh-52(yum1346) srh-55(yum1347) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1046 Species: C. elegans Genotype: str-52(yum1348) str-55(yum1349) str-56(yum1350) str-57(yum1351) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1047 Species: C. elegans Genotype: srh-33(yum1352) srh-34(yum1353) srh-35(yum1354) srh-36(yum1355) srh-37(yum1356) srh-38(yum1357) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1048 Species: C. elegans Genotype: str-245(yum1362) I; str-238(yum1358) str-240(yum1359) str-243(yum1360) str-244(yum1361) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1049 Species: C. elegans Genotype: str-176(yum1363) IV; str-177(yum1364) str-178(yum1365) str-180(yum1366) str-181(yum1367) str-182(yum1368) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1050 Species: C. elegans Genotype: srxa-10(yum1371) III; srxa-11(yum1372) srxa-19(yum1373) V; srxa-8(yum1369) srxa-9(yum1370) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1051 Species: C. elegans Genotype: srw-51(yum1374) srw-53(yum1375) srw-54(yum1376) srw-55(yum1377) t27c5.10(yum1378) y70c5a.4(yum1379) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1052 Species: C. elegans Genotype: sre-13(yum1380) II; sre-14(yum1381) sre-15(yum1382) sre-16(yum1383) sre-18(yum1384) sre-19(yum1385) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1053 Species: C. elegans Genotype: c29f9.8(yum1387) III; t26h8.4(yum1386) y75b12b.10(yum1388) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1054 Species: C. elegans Genotype: srd-17(yum1389) srd-18(yum1390) srd-19(yum1391) srd-20(yum1392) srd-21(yum1393) srd-32(yum1394) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1055 Species: C. elegans Genotype: str-250(yum1395) str-252(yum1396) str-253(yum1397) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1056 Species: C. elegans Genotype: sre-1(yum1398) sre-2(yum1399) sre-3(yum1400) II; sre-4(yum1401) sre-5(yum1402) IV; sre-6(yum1403) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1057 Species: C. elegans Genotype: npr-1(yum1034) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1058 Species: C. elegans Genotype: npr-12(yum1406) IV; npr-9(yum1404) npr-11(yum1405) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1059 Species: C. elegans Genotype: sru-37(yum1407) sru-38(yum1408) sru-39(yum1409) sru-44(yum1410) sru-45(yum1411) c45h4.18(yum1412) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1060 Species: C. elegans Genotype: srx-12(yum1413) srx-13(yum1414) srx-14(yum1415) IV; srx-33(yum1416) srx-34(yum1417) srx-36(yum1418) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1061 Species: C. elegans Genotype: tkr-1(yum1419) III; tkr-2(yum1420) tkr-3(yum1421) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1062 Species: C. elegans Genotype: npr-6(yum1027) npr-7(yum1028) npr-8(yum1029) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1063 Species: C. elegans Genotype: npr-14(yum1423) I; npr-15(yum1424) III; npr-13(yum1422) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1064 Species: C. elegans Genotype: npr-20(yum1426) II; npr-18(yum1425) npr-21(yum1427) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1065 Species: C. elegans Genotype: npr-26(yum1429) npr-27(yum1430) IV; npr-25(yum1428) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1066 Species: C. elegans Genotype: srj-1(yum1431) IV; srj-4(yum1432) srj-5(yum1433) srj-6(yum1434) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1067 Species: C. elegans Genotype: str-96(yum1435) str-97(yum1436) str-99(yum1437) str-101(yum1438) str-102(yum1439) str-103(yum1440) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1068 Species: C. elegans Genotype: sri-5(yum1445) I; sri-18(yum1441) V; sri-19(yum1442) IV; sri-20(yum1443) sri-21(yum1444) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1069 Species: C. elegans Genotype: npr-31(yum1446) II; npr-32(yum1447) IV; npr-33(yum1448) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1070 Species: C. elegans Genotype: sru-13(yum1452) sru-12(yum1451) sru-11(yum1450) sru-9(yum1449) sru-15(yum1453) IV; sru-40(yum1454) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1071 Species: C. elegans Genotype: sra-1(yum1455) sra-10(yum1456) sra-11(yum1457) sra-12(yum1458) sra-13(yum1459) sra-14(yum1460) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1072 Species: C. elegans Genotype: npr-17(yum1462) III; npr-16(yum1461) npr-18(yum1463) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1073 Species: C. elegans Genotype: srt-1(yum1464) srt-2(yum1465) srt-3(yum1466) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1074 Species: C. elegans Genotype: srxa-5(yum1470) III; srxa-2(yum1468) IV; srxa-4(yum1469) srxa-7(yum1472) srxa-1(yum1467) srxa-6(yum1471) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1075 Species: C. elegans Genotype: str-166(yum1476) str-169(yum1477) str-163(yum1473) str-164(yum1474) IV; str-165(yum1475) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1076 Species: C. elegans Genotype: ntr-1(yum1478) I; ntr-2(yum1479) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1077 Species: C. elegans Genotype: srz-74(yum1480) srz-75(yum1481) srz-78(yum1482) srz-79(yum1483) srz-82(yum1484) srz-83(yum1485) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1078 Species: C. elegans Genotype: str-71(yum1486) str-73(yum1487) V; str-74(yum1488) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1079 Species: C. elegans Genotype: npr-34(yum1491) II; npr-35(yum1492) npr-22(yum1489) IV; npr-24(yum1490) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1080 Species: C. elegans Genotype: npr-29(yum1494) npr-30(yum1495) III; npr-28(yum1493) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1081 Species: C. elegans Genotype: sra-32(yum1501) sra-27(yum1496) sra-28(yum1497) sra-29(yum1498) sra-31(yum1500) II; sra-30(yum1499) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1082 Species: C. elegans Genotype: sprr-3(yum1504) IV; sprr-1(yum1502) sprr-2(yum1503) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1083 Species: C. elegans Genotype: ckr-1(yum1505) I; ckr-2(yum1506) III. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1084 Species: C. elegans Genotype: frpr-5(yum1507) frpr-6(yum1508) frpr-11(yum1509) frpr-12(yum1510) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1085 Species: C. elegans Genotype: daf-37(yum1511) II; daf-38(yum1512) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1086 Species: C. elegans Genotype: ser-5(yum1515) I; ser-4(yum1514) III; ser-7(yum1516) ser-1(yum1513) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1087 Species: C. elegans Genotype: ser-3(yum1518) I; ser-6(yum1519) IV; octr-1(yum1517) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1088 Species: C. elegans Genotype: tyra-3(yum1522) tyra-2(yum1521) ser-2(yum1520) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1089 Species: C. elegans Genotype: egl-6(yum1523) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1090 Species: C. elegans Genotype: ador-1(yum1524) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1091 Species: C. elegans Genotype: mig-1(yum1525) I; cfz-2(yum1526) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1092 Species: C. elegans Genotype: srsx-35(yum1527) srsx-36(yum1528) srsx-37(yum1529) srsx-38(yum1530) srsx-39(yum1531) srsx-40(yum1532) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1093 Species: C. elegans Genotype: sre-37(yum1533) sre-38(yum1536) sre-39(yum1537) sre-40(yum1538) sre-41(yum1534) sre-42(yum1535) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1094 Species: C. elegans Genotype: srh-277(yum1542) srh-283(yum1539) srh-284(yum1540) srh-286(yum1541) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1095 Species: C. elegans Genotype: srbc-12(yum1543) srbc-13(yum1544) srbc-14(yum1545) srbc-16(yum1546) srbc-17(yum1547) srbc-19(yum1548) srbc-20(yum1549) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1096 Species: C. elegans Genotype: srg-69(yum1553) II; srg-3(yum1554) srg-4(yum1555) III; srg-38(yum1550) srg-54(yum1551) srg-55(yum1552) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1097 Species: C. elegans Genotype: srg-31(yum1556) srg-32(yum1557) srg-33(yum1558) srg-34(yum1559) V; srg-36(yum1560) srg-37(yum1561) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1098 Species: C. elegans Genotype: str-12(yum1563) str-13(yum1564) str-106(yum1565) str-108(yum1566) str-109(yum1567) str-111(yum1568) f10a3.12(yum1569) k05d4.9(yum1570) V; str-10(yum1562) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1099 Species: C. elegans Genotype: srh-57(yum1575) srh-59(yum1572) srh-60(yum1573) srh-305(yum1574) II; srh-56(yum1571) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1100 Species: C. elegans Genotype: sre-29(yum1576) sre-30(yum1577) sre-31(yum1578) sre-34(yum1579) sre-35(yum1580) II; sre-36(yum1581) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1101 Species: C. elegans Genotype: sra-17(yum1582) sra-18(yum1583) sra-20(yum1584) sra-21(yum1585) sra-22(yum1586) sra-23(yum1587) sra-24(yum1588) sra-25(yum1589) I; sra-26(yum1590) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1102 Species: C. elegans Genotype: b0244.10(yum1591) b0244.15(yum1592) b0244.16(yum1593) b0244.17(yum1594) b0244.4(yum1595) b0244.5(yum1596) b0244.7(yum1597) III. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1103 Species: C. elegans Genotype: lat-2(yum1599) mth-1(yum1600) mth-2(yum1601) II; fmi-1(yum1598) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1104 Species: C. elegans Genotype: srv-5(yum1602) srv-6(yum1603) srv-7(yum1604) X; srv-8(yum1605) srv-9(yum1606) srv-10(yum1607) c34b4.3(yum1608) V; b0563.6(yum1609) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1105 Species: C. elegans Genotype: srab-6(yum1610) srab-7(yum1611) srab-8(yum1612) srab-9(yum1613) srab-10(yum1614) srab-11(yum1615) srab-20(yum1616) srab-21(yum1617) srab-22(yum1618) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1106 Species: C. elegans Genotype: srj-42(yum1624) IV; srj-53(yum1630) srj-52(yum1629) srj-37(yum1620) srj-38(yum1621) srj-50(yum1628) srj-45(yum1626) srj-44(yum1625) srj-49(yum1627) srj-57(yum1633) srj-55(yum1632) srj-54(yum1631) srj-40(yum1623) srj-39(yum1622) srj-33(yum1619) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1107 Species: C. elegans Genotype: srx-19(yum1634) IV; srx-24(yum1635) srx-26(yum1636) srx-28(yum1637) srx-29(yum1638) srx-31(yum1639) srx-32(yum1640) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1108 Species: C. elegans Genotype: srr-7(yum1641) srr-8(yum1642) srr-9(yum1643) srr-10(yum1644) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1109 Species: C. elegans Genotype: frpr-1(yum1645) frpr-2(yum1646) X; frpr-14(yum1647) II; frpr-17(yum1648) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1110 Species: C. elegans Genotype: srt-63(yum1649) srt-64(yum1650) srt-65(yum1651) srt-67(yum1652) srt-68(yum1653) srt-69(yum1654) srt-70(yum1655) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1111 Species: C. elegans Genotype: srsx-14(yum1656) srsx-15(yum1657) srsx-16(yum1658) IV; srsx-17(yum1659) srsx-18(yum1660) srsx-19(yum1661) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1112 Species: C. elegans Genotype: srz-58(yum1662) V; srz-59(yum1663) srz-60(yum1664) srz-61(yum1665) srz-62(yum1666) IV; srz-85(yum1667) I. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1113 Species: C. elegans Genotype: srw-16(yum1668) srw-17(yum1669) srw-19(yum1670) srw-20(yum1671) srw-21(yum1672) srw-22(yum1673) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1114 Species: C. elegans Genotype: srw-108(yum1674) V; srw-111(yum1675) I; srw-112(yum1676) srw-113(yum1677) V; m01g12.6(yum1678) m01g12.16(yum2916) I. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1115 Species: C. elegans Genotype: srg-1(yum1684) srg-11(yum1679) III; srg-14(yum1680) I; srg-15(yum1681) srg-16(yum1682) srg-17(yum1683) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1116 Species: C. elegans Genotype: srr-1(yum1685) srr-2(yum1686) srr-3(yum1687) srr-4(yum1688) srr-6(yum1689) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1117 Species: C. elegans Genotype: srbc-54(yum1690) srbc-55(yum1691) srbc-56(yum1692) srbc-57(yum1693) srbc-66(yum1694) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1118 Species: C. elegans Genotype: sre-50(yum1695) sre-52(yum1696) sre-53(yum1697) sre-54(yum1698) sre-55(yum1699) sre-56(yum1700) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1119 Species: C. elegans Genotype: gur-4(yum1701) gur-5(yum1702) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1120 Species: C. elegans Genotype: fshr-1(yum1706) V; npr-42(yum1703) II; npr-36(yum1704) pcdr-1(yum1707) X; tag-89(yum1705) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1121 Species: C. elegans Genotype: npr-19(yum1708) X; npr-23(yum1709) I; h23l24.4(yum1711) t02d1.4(yum1712) IV; zk813.5(yum1710) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1122 Species: C. elegans Genotype: srz-69(yum1715) srz-70(yum1716) srz-71(yum1717) srz-104(yum1713) srz-105(yum1714) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1123 Species: C. elegans Genotype: srd-33(yum1718) srd-34(yum1719) srd-35(yum1720) V; srd-36(yum1721) srd-38(yum1722) srd-39(yum1723) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1124 Species: C. elegans Genotype: t06e4.7(yum1724) V; y32h12a.1(yum1726) III; y77e11a.16(yum1725) IV; zk6.6(yum1728) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1125 Species: C. elegans Genotype: f52d10.4(yum1729) f56a12.2(yum1730) m04g7.3(yum1731) ah9.4(yum1732) f54e4.2(yum1733) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1126 Species: C. elegans Genotype: dmsr-1(yum1734) V; dmsr-2(yum1735) I; dmsr-3(yum1736) II; dmsr-8(yum1737) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1127 Species: C. elegans Genotype: dmsr-4(yum1738) dmsr-6(yum1740) II; dmsr-5(yum1739) III; dmsr-7(yum1741) dmsr-8(yum1742) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1128 Species: C. elegans Genotype: srg-39(yum1743) srg-40(yum1744) srg-41(yum1745) srg-42(yum1746) srg-44(yum1747) srg-45(yum1748) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1129 Species: C. elegans Genotype: srt-54(yum1749) II; srt-55(yum1750) III; srt-56(yum1751) V; srt-61(yum1752) srt-62(yum1753) I; srt-73(yum1754) IV; f49d11.11(yum1755) I. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1130 Species: C. elegans Genotype: srz-25(yum1757) V; srz-29(yum1758) srz-31(yum1759) srz-32(yum1760) srz-37(yum1761) srz-38(yum1762) srz-72(yum1762) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1131 Species: C. elegans Genotype: egl-47(yum1765) V; lite-1(yum1763) gur-3(yum1764) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1132 Species: C. elegans Genotype: srh-247(yum1770) srh-248(yum1771) V; srh-250(yum1766) II; srh-251(yum1767) srh-252(yum1768) srh-255(yum1769) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1133 Species: C. elegans Genotype: srx-38(yum1772) srx-39(yum1773) srx-41(yum1774) c03g6.21(yum1775) srx-42(yum1776) srx-43(yum1777) fbxa-199(yum1778) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1134 Species: C. elegans Genotype: srj-21(yum1779) srj-25(yum1780) srj-26(yum1781) srj-27(yum1782) srj-29(yum1783) srj-32(yum1784) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1135 Species: C. elegans Genotype: srx-1(yum1785) srx-2(yum1786) srx-3(yum1787) srx-4(yum1788) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1136 Species: C. elegans Genotype: srz-42(yum1789) IV; srz-44(yum1790) srz-47(yum1791) srz-48(yum1792) srz-54(yum1793) V; srz-56(yum1794) III. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1137 Species: C. elegans Genotype: srsx-8(yum1795) srsx-9(yum1796) srsx-10(yum1797) srsx-11(yum1798) srsx-12(yum1799) srsx-13(yum1800) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1138 Species: C. elegans Genotype: srsx-31(yum1801) srsx-32(yum1802) srsx-33(yum1803) srsx-34(yum1804) f54e2.5(yum1806) f54e2.9(yum1805) t09e8.4(yum1807) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1139 Species: C. elegans Genotype: srz-16(yum1808) srz-18(yum1809) V; srz-19(yum1810) srz-20(yum1811) srz-23(yum1813) srz-24(yum1812) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1140 Species: C. elegans Genotype: srw-72(yum1814) srw-76(yum1816) srw-77(yum1817) V; srw-79(yum1818) II; srw-82(yum1819) srw-83(yum1820) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1141 Species: C. elegans Genotype: srx-101(yum1821) srx-102(yum1822) srx-104(yum1823) srx-105(yum1824) srx-108(yum1825) srx-110(yum1826) srx-111(yum1827) srx-112(yum1828) II; srx-113(yum1829) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1142 Species: C. elegans Genotype: c04c3.6(yum1830) c04c3.7(yum1835) IV; c50h11.13(yum1832) c54e10.3(yum1833) V; t01b11.1(yum1834) IV; zk863.1(yum1831) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1143 Species: C. elegans Genotype: srab-12(yum1836) V; srab-14(yum1837) II; srab-16(yum1838) srab-17(yum1839) srab-18(yum1840) srab-24(yum1841) srab-25(yum1842) srab-26(yum1843) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1144 Species: C. elegans Genotype: npr-40(yum1846) ah9.1(yum1845) ah9.4(yum1848) b0563.6(yum1844) c17h11.1(yum1847) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1145 Species: C. elegans Genotype: srab-1(yum1849) srab-2(yum1850) srab-3(yum1851) srab-4(yum1852) srab-13(yum1853) srab-23(yum1854) V; k08b5.1(yum1856) X; y41d4b.1(yum1855) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1146 Species: C. elegans Genotype: srx-48(yum1857) V; srx-50(yum1858) IV; srx-51(yum1859) srx-54(yum1860) srx-59(yum1862) srx-64(yum1863) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1147 Species: C. elegans Genotype: srx-5(yum1864) srx-6(yum1865) srx-7(yum1866) srx-8(yum1867) srx-9(yum1868) srx-10(yum1869) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1148 Species: C. elegans Genotype: fmil-1(yum1873) IV; gpr-158(yum1872) I; tmem-145(yum1871) t04f8.2(yum1870) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1149 Species: C. elegans Genotype: srh-1(yum1874) srh-2(yum1875) srh-3(yum1876) srh-4(yum1877) srh-5(yum1878) srh-7(yum1879) srh-8(yum1880) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1150 Species: C. elegans Genotype: srh-125(yum1881) srh-269(yum1882) srh-270(yum1883) srh-271(yum1884) srh-272(yum1885) srh-274(yum1886) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1151 Species: C. elegans Genotype: srh-274(yum1887) srh-275(yum1888) srh-276(yum1889) srh-277(yum1890) srh-279(yum1891) srh-281(yum1892) srh-282(yum1893) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1152 Species: C. elegans Genotype: srbc-50(yum1894) srbc-51(yum1895) srbc-67(yum1896) srbc-68(yum1897) srbc-70(yum1898) srbc-71(yum1899) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1153 Species: C. elegans Genotype: srsx-21(yum1900) srsx-22(yum1901) srsx-24(yum1902) srsx-25(yum1903) srsx-26(yum1904) srsx-27(yum1905) srsx-28(yum1906) srsx-29(yum1907) srsx-30(yum1908) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1154 Species: C. elegans Genotype: srx-116(yum1909) srx-117(yum1910) srx-118(yum1911) srx-119(yum1912) srx-120(yum1913) srx-121(yum1914) srx-122(yum1915) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1155 Species: C. elegans Genotype: str-32(yum1916) str-37(yum1917) str-38(yum1918) str-39(yum1919) str-40(yum1920) str-41(yum1921) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1156 Species: C. elegans Genotype: srw-84(yum1922) srw-85(yum1923) srw-86(yum1925) srw-87(yum1926) V; srw-88(yum1927) I; c25f9.13(yum1924) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1157 Species: C. elegans Genotype: npr-1(yum1928) npr-2(yum1929) npr-3(yum1930) npr-4(yum1931) npr-5(yum1932) npr-10(yum1933) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1158 Species: C. elegans Genotype: srw-66(yum1935) srw-67(yum1936) srw-68(yum1937) srw-69(yum1938) srw-71(yum1939) srw-73(yum1940) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1159 Species: C. elegans Genotype: srh-135(yum1941) srh-136(yum1942) srh-137(yum1943) srh-138(yum1944) srh-139(yum1945) srh-140(yum1946) srh-141(yum1947) srh-142(yum1948) srh-145(yum1949) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1160 Species: C. elegans Genotype: e04d5.2(yum1950) c54d10.5(yum1951) t11f9.1(yum1952) y41d4b.24(yum1953) f32d8.10(yum1954) y37e11al.1(yum1955) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1161 Species: C. elegans Genotype: y6e2a.1(yum1956) c03h5.7(yum1957) k10c9.4(yum1958) c03h5.9(yum1959) t01g5.6(yum1960) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1162 Species: C. elegans Genotype: srv-11(yum1961) srv-12(yum1962) srv-13(yum1963) srv-14(yum1964) srv-15(yum1965) srv-16(yum1966) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1163 Species: C. elegans Genotype: srh-128(yum1967) srh-129(yum1968) srh-130(yum1969) srh-131(yum1970) srh-132(yum1971) srh-133(yum1972) srh-134(yum1973) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1164 Species: C. elegans Genotype: srx-73(yum1974) srx-74(yum1975) srx-76(yum1976) srx-77(yum1977) srx-78(yum1978) srx-80(yum1979) srx-82(yum1980) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1165 Species: C. elegans Genotype: c30g7.5(yum1981) f41h8.1(yum1982) f41h8.2(yum1983) k09c6.10(yum1984) r13d11.1(yum1985) r13d11.3(yum1986) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1166 Species: C. elegans Genotype: str-78(yum1987) IV; str-79(yum1988) X; str-81(yum1989) str-82(yum1990) str-83(yum1991) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1167 Species: C. elegans Genotype: dmsr-9(yum1992) dmsr-10(yum1993) dmsr-11(yum1994) dmsr-12(yum1995) dmsr-13(yum1996) dmsr-14(yum1997) dmsr-15(yum1998) dmsr-16(yum1999) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1168 Species: C. elegans Genotype: srh-257(yum2003) srh-258(yum2004) srh-264(yum2000) srh-265(yum2001) srh-266(yum2002) srh-268(yum2005) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1169 Species: C. elegans Genotype: srd-10(yum2006) IV; srd-11(yum2007) V; srd-12(yum2008) IV; srd-13(yum2009) IV; srd-15(yum2010) srd-16(yum2011) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1170 Species: C. elegans Genotype: srz-63(yum2012) srz-64(yum2013) II; srz-66(yum2014) V; srz-67(yum2015) II; srz-90(yum2016) srz-91(yum2017) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1171 Species: C. elegans Genotype: srx-84(yum2018) srx-85(yum2019) srx-86(yum2020) srx-87(yum2021) srx-88(yum2022) srx-90(yum2023) srx-92(yum2024) srx-93(yum2025) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1172 Species: C. elegans Genotype: str-13(yum2029) str-14(yum2026) str-15(yum2027) str-16(yum2028) str-18(yum2030) str-19(yum2031) str-20(yum2032) str-23(yum2033) str-25(yum2034) f22b8.3(yum2035) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1173 Species: C. elegans Genotype: odr-10(yum2055) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1174 Species: C. elegans Genotype: c31b8.16(yum2037) t26h8.5(yum2036) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1175 Species: C. elegans Genotype: srg-46(yum2038) srg-47(yum2039) V; srg-48(yum2040) srg-50(yum2041) srg-51(yum2042) IV; srg-53(yum2043) V; y54g2a.38(yum2045) y54g2a.77(yum2044) IV; ah9.1(yum2046) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1176 Species: C. elegans Genotype: str-170(yum2047) str-171(yum2048) str-172(yum2049) str-173(yum2050) str-174(yum2051) str-175(yum2052) str-166(yum2053) y9c9a.5(yum2054) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1177 Species: C. elegans Genotype: srh-243(yum2056) srh-244(yum2057) srh-245(yum2058) srh-241(yum2059) srh-246(yum2060) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1178 Species: C. elegans Genotype: aex-2(yum2061) aexr-1(yum2062) aexr-2(yum2063) aexr-3(yum2064) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1179 Species: C. elegans Genotype: f49c5.9(yum2065) c11h1.2(yum2066) y75b8a.16(yum2067) y62h9a.1(yum2068) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1180 Species: C. elegans Genotype: srw-23(yum2069) srw-24(yum2070) srw-26(yum2071) srw-29(yum2072) srw-31(yum2073) srw-32(yum2074) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1181 Species: C. elegans Genotype: sra-33(yum2076) sra-34(yum2077) sra-35(yum2078) sra-36(yum2079) sra-37(yum2080) sra-38(yum2081) sra-39(yum2082) k11e4.6(yum2083) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1182 Species: C. elegans Genotype: str-196(yum2084) str-198(yum2085) str-199(yum2086) str-200(yum2087) str-204(yum2088) str-205(yum2089) str-193(yum2090) str-2(yum2091) str-3(yum2092) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1183 Species: C. elegans Genotype: str-185(yum2093) str-188(yum2094) str-187(yum2095) str-190(yum2096) str-178(yum2097) y116a8c.40(yum2098) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1184 Species: C. elegans Genotype: f40g9.15(yum2099) c35d6.13(yum2100) f47d2.11(yum2101) zk218.4(yum2102) zc132.3(yum2103) zc132.11(yum2104) III. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1185 Species: C. elegans Genotype: f49e11.2(yum2105) f57c9.6(yum2106) zc266.1(yum2107) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1186 Species: C. elegans Genotype: srd-63(yum2108) srd-64(yum2108) srd-65(yum2110) srd-66(yum2111) srd-67(yum2112) srd-68(yum2113) srd-69(yum2114) srd-70(yum2115) srd-74(yum2116) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1187 Species: C. elegans Genotype: srx-16(yum2117) srx-17(yum2118) srx-18(yum2119) srx-20(yum2120) srx-21(yum2121) srx-22(yum2122) srx-23(yum2123) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1188 Species: C. elegans Genotype: srw-1(yum2124) srw-2(yum2125) srw-3(yum2126) srw-4(yum2127) srw-6(yum2128) srw-7(yum2129) srw-8(yum2130) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1189 Species: C. elegans Genotype: str-138(yum2131) str-139(yum2132) str-140(yum2133) str-143(yum2134) str-144(yum2135) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1190 Species: C. elegans Genotype: srd-1(yum2136) srd-2(yum2137) srd-3(yum2138) srd-4(yum2139) srd-5(yum2140) srd-7(yum2141) srd-8(yum2142) srd-9(yum2143) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1191 Species: C. elegans Genotype: srw-127(yum2144) srw-129(yum2145) srw-136(yum2146) srw-133(yum2147) srw-134(yum2148) srw-144(yum2149) srw-145(yum2150) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1192 Species: C. elegans Genotype: srg-10(yum2151) srg-13(yum2152) srg-20(yum2153) srg-21(yum2154) srg-22(yum2155) srg-23(yum2156) srg-24(yum2157) III. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1193 Species: C. elegans Genotype: sri-40(yum2158) sri-42(yum2159) sri-43(yum2160) sri-45(yum2161) sri-46(yum2162) sri-47(yum2163) sri-48(yum2164) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1194 Species: C. elegans Genotype: f36d4.4(yum2165) zk721.4(yum2166) b0334.6(yum2167) c09f12.3(yum2168) f13h6.5(yum2169) b0244.6(yum2170) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1195 Species: C. elegans Genotype: y52b11a.11(yum2171) I. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1196 Species: C. elegans Genotype: srd-23(yum2172) srd-25(yum2173) srd-26(yum2174) srd-27(yum2175) srd-28(yum2176) srd-29(yum2177) srd-30(yum2178) srd-31(yum2179) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1197 Species: C. elegans Genotype: y62h9a.10(yum2180) y40b10a.5(yum2181) c30b5.7(yum2182) f09c12.6(yum2183) r02d5.6(yum2184) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1198 Species: C. elegans Genotype: srv-1(yum2185) srv-2(yum2186) srv-3(yum2187) srv-4(yum2188) srv-17(yum2189) srv-19(yum2190) srv-21(yum2191) srv-22(yum2192) srv-34(yum2193) III. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1199 Species: C. elegans Genotype: srw-130(yum2194) srw-139(yum2195) srw-140(yum2196) srw-141(yum2197) srw-142(yum2198) srw-143(yum2199) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1200 Species: C. elegans Genotype: srd-53(yum2200) srd-54(yum2201) srd-55(yum2202) srd-56(yum2203) srd-58(yum2204) srd-59(yum2205) I. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1201 Species: C. elegans Genotype: str-43(yum2206) str-44(yum2207) str-45(yum2208) str-46(yum2209) str-47(yum2210) str-48(yum2211) str-38(yum2212) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1202 Species: C. elegans Genotype: sre-26(yum2213) sre-27(yum2214) sre-28(yum2215) sre-32(yum2216) sre-33(yum2217) sre-49(yum2218) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1203 Species: C. elegans Genotype: t27e7.9(yum1046) t27e7.4(yum1047) t27e7.5(yum1048) t27e7.3(yum1049) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1204 Species: C. elegans Genotype: t03e6.9(yum2219) t03e6.8(yum2220) y37h2c.4(yum2221) c53d6.11(yum2222) w06g6.15(yum2223) y70c5b.2(yum2224) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1205 Species: C. elegans Genotype: srw-56(yum2225) srw-57(yum2226) srw-59(yum2227) srw-60(yum2228) srw-61(yum2229) srw-62(yum2230) srw-63(yum2231) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1206 Species: C. elegans Genotype: srt-19(yum2232) srt-20(yum2233) srt-22(yum2234) srt-15(yum2235) srt-16(yum2236) srt-17(yum2237) srt-18(yum2238) srt-23(yum2239) srt-24(yum2240) srt-59(yum2241) y55f3c.10(yum2242) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1207 Species: C. elegans Genotype: dcar-1(yum2244) pcdr-1(yum2245) d2023.1(yum2246) gtr-1(yum2247) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1208 Species: C. elegans Genotype: srw-33(yum2248) srw-34(yum2249) srw-35(yum2250) srw-36(yum2251) srw-38(yum2252) srw-39(yum2253) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1209 Species: C. elegans Genotype: f57g9.7(yum2254) r05f9.7(yum2255) c54c6.4(yum2256) r11g10.3(yum2257) f27e5.5(yum2258) f27e5.8(yum2259) t10g3.2(yum2260) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1210 Species: C. elegans Genotype: srsx-1(yum2261) srsx-2(yum2262) srsx-3(yum2263) srsx-5(yum2264) srsx-6(yum2265) srsx-7(yum2266) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1211 Species: C. elegans Genotype: srd-45(yum2267) srd-46(yum2268) srd-47(yum2269) srd-48(yum2270) srd-50(yum2271) srd-51(yum2272) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1212 Species: C. elegans Genotype: gnrr-1(yum2273) gnrr-2(yum2274) gnrr-3(yum2275) gnrr-4(yum2276) gnrr-5(yum2277) gnrr-6(yum2278) gnrr-7(yum2279) I. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1213 Species: C. elegans Genotype: str-85(yum2280) str-86(yum2281) str-87(yum2282) str-84(yum2283) str-88(yum2284) str-89(yum2285) str-90(yum2286) str-92(yum2287) str-93(yum2288) str-94(yum2289) str-264(yum2290) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1214 Species: C. elegans Genotype: sre-7(yum2291) sre-8(yum2292) sre-9(yum2293) sre-10(yum2294) sre-11(yum2295) sre-12(yum2296) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1215 Species: C. elegans Genotype: sre-20(yum2297) sre-21(yum2298) sre-22(yum2299) sre-23(yum2300) sre-24(yum2301) sre-51(yum2302) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1216 Species: C. elegans Genotype: srbc-58(yum2303) srbc-59(yum2304) srbc-60(yum2305) srbc-64(yum2306) srbc-66(yum2307) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1217 Species: C. elegans Genotype: sru-16(yum2308) sru-17(yum2309) sru-18(yum2310) sru-19(yum2311) sru-20(yum2312) sru-42(yum2313) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1218 Species: C. elegans Genotype: sru-21(yum2314) sru-22(yum2315) sru-23(yum2316) sru-24(yum2317) sru-25(yum2318) sru-26(yum2319) sru-27(yum2320) sru-28(yum2321) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1219 Species: C. elegans Genotype: srw-40(yum2322) srw-41(yum2323) srw-42(yum2324) srw-43(yum2325) srw-44(yum2326) srw-48(yum2327) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1220 Species: C. elegans Genotype: srm-1(yum2328) srm-2(yum2329) srm-3(yum2330) srm-4(yum2331) srm-5(yum2332) srm-6(yum2333) srn-1(yum2334) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1221 Species: C. elegans Genotype: str-247(yum2335) str-248(yum2336) str-263(yum2337) str-249(yum2338) str-246(yum2339) str-252(yum2340) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1222 Species: C. elegans Genotype: f27e5.8(yum2341) t10g3.4(yum2342) t10g3.2(yum2343) y57g11c.46(yum2344) y57g11c.28(yum2345) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1223 Species: C. elegans Genotype: f38e1.6(yum2346) f09c6.16(yum2347) k01a6.6(yum2348) dop-6(yum2349) c24a8.6(yum2350) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1224 Species: C. elegans Genotype: srz-10(yum2351) srz-11(yum2352) srz-12(yum2353) srz-13(yum2354) srz-14(yum2355) srz-15(yum2356) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1225 Species: C. elegans Genotype: srw-89(yum2357) srw-90(yum2358) srw-91(yum2359) srw-94(yum2360) srw-95(yum2361) srw-97(yum2362) srw-98(yum2363) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1226 Species: C. elegans Genotype: srxa-12(yum2364) srxa-13(yum2365) srxa-14(yum2366) srxa-15(yum2367) srxa-16(yum2368) srxa-18(yum2369) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1227 Species: C. elegans Genotype: srbc-40(yum2370) srbc-41(yum2371) srbc-43(yum2372) srbc-44(yum2373) srbc-45(yum2374) srbc-48(yum2375) srbc-49(yum2376) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1228 Species: C. elegans Genotype: srx-95(yum2377) srx-96(yum2378) srx-97(yum2379) srx-98(yum2380) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1229 Species: C. elegans Genotype: sru-1(yum2381) sru-2(yum2382) sru-3(yum2383) sru-4(yum2384) sru-6(yum2385) sru-8(yum2386) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1230 Species: C. elegans Genotype: srb-6(yum2387) srb-7(yum2388) srb-8(yum2389) srb-11(yum2390) srb-15(yum2391) srb-16(yum2392) srb-17(yum2393) srb-18(yum2394) srb-19(yum2395) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1231 Species: C. elegans Genotype: str-206(yum2396) str-207(yum2397) str-208(yum2398) str-209(yum2399) str-211(yum2400) str-214(yum2401) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1232 Species: C. elegans Genotype: srx-114(yum2402) srx-115(yum2403) srx-125(yum2404) srx-128(yum2405) srx-129(yum2406) srx-131(yum2407) srx-136(yum2408) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1233 Species: C. elegans Genotype: str-229(yum2409) str-230(yum2410) str-231(yum2411) str-232(yum2412) str-233(yum2413) str-236(yum2414) c06b3.1(yum2415) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1234 Species: C. elegans Genotype: sra-2(yum2416) sra-3(yum2417) sra-4(yum2418) sra-6(yum2419) sra-7(yum2420) sra-8(yum2421) sra-9(yum2422) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1235 Species: C. elegans Genotype: srv-24(yum2423) srv-25(yum2424) srv-26(yum2425) srv-27(yum2426) srv-28(yum2427) srv-29(yum2428) srv-30(yum2429) srv-31(yum2430) srv-32(yum2431) ops-1(yum2432) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1236 Species: C. elegans Genotype: sri-66(yum2433) sri-67(yum2434) sri-69(yum2435) sri-70(yum2436) sri-71(yum2437) sri-72(yum2438) sri-73(yum2439) sri-74(yum2440) sri-77(yum2441) y61b8a.6(yum2442) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1237 Species: C. elegans Genotype: sri-10(yum2443) sri-11(yum2444) sri-12(yum2445) sri-13(yum2446) sri-14(yum2447) sri-15(yum2448) sri-16(yum2449) sri-17(yum2450) sri-6(yum2451) sri-7(yum2452) sri-9(yum2453) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1238 Species: C. elegans Genotype: f07b10.4(yum2454) f07b10.7(yum2455) f59b1.6(yum2456) zk418.7(yum2457) w08g11.5(yum2458) r10e8.5(yum2459) zk418.6(yum2460) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1239 Species: C. elegans Genotype: srz-94(yum2461) srz-95(yum2462) srz-96(yum2463) srz-97(yum2464) srz-99(yum2465) srz-100(yum2466) srz-101(yum2467) srz-102(yum2468) srz-103(yum2469) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1240 Species: C. elegans Genotype: t01d1.5(yum2470) zk1307.7(yum2471) f59b1.4(yum2472) f18e3.10(yum2473) f07c3.16(yum2474) f45e10.2(yum2475)f59b1.6(yum2476) y32h12a.1(yum2477) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1241 Species: C. elegans Genotype: srd-40(yum2478) srd-41(yum2479) srd-42(yum2480) srd-43(yum2481) srd-44(yum2482) srd-49(yum2483) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1242 Species: C. elegans Genotype: srx-56(yum2484) srx-58(yum2485) srx-60(yum2486) srx-62(yum2487) srx-63(yum2488) srx-65(yum2489) srx-66(yum2490) srx-67(yum2491) srx-68(yum2492) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1243 Species: C. elegans Genotype: srh-185(yum2493) srh-186(yum2494) srh-187(yum2495) srh-190(yum2496) srh-192(yum2497) srh-193(yum2498) srh-194(yum2499) srh-195(yum2500) srh-199(yum2501) srh-200(yum2502) srh-201(yum2503) srh-203(yum2504) srh-204(yum2505) srh-206(yum2506) srh-207(yum2507) srh-208(yum2508) srh-209(yum2509) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1244 Species: C. elegans Genotype: srh-231(yum2510) srh-233(yum2511) srh-234(yum2512) srh-235(yum2513) srh-236(yum2514) srh-237(yum2515) srh-239(yum2516) srh-240(yum2517) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1245 Species: C. elegans Genotype: srx-130(yum2518) srx-133(yum2519) srx-134(yum2520) srx-135(yum2521) srx-137(yum2522) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1246 Species: C. elegans Genotype: srbc-75(yum2851) srbc-76(yum2852) srbc-77(yum2853) srbc-78(yum2854) srbc-79(yum2855) srbc-82(yum2856) srbc-83(yum2857) srbc-84(yum2858) srbc-81(yum2859) y19d10a.17(yum2860) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1247 Species: C. elegans Genotype: srh-10(yum2523) srh-15(yum2524) srh-16(yum2525) srh-18(yum2526) srh-17(yum2527) srh-11(yum2528) srh-20(yum2529) srh-21(yum2530) srh-22(yum2531) srh-23(yum2532) srh-19(yum2533) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1248 Species: C. elegans Genotype: str-255(yum2534) str-256(yum2535) str-257(yum2536) str-260(yum2537) str-254(yum2538) str-261(yum2539) str-262(yum2540) y19d10a.1(yum2541) str-258(yum2542) y19d10a.2(yum2543) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1249 Species: C. elegans Genotype: sri-33(yum2544) sri-34(yum2545) sri-36(yum2546) sri-32(yum2547) sri-37(yum2548) sri-38(yum2549) sri-39(yum2550) sri-40(yum2551) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1250 Species: C. elegans Genotype: srz-1(yum2552) V; srz-3(yum2553) srz-4(yum2554) I; srz-5(yum2555) srz-6(yum2556) II; srz-8(yum2557) srz-9(yum2558) III. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 07/25/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1251 Species: C. elegans Genotype: srbc-21(yum2559) srbc-22(yum2560) srbc-23(yum2561) srbc-24(yum2562) srbc-25(yum2563) srbc-26(yum2564) srbc-27(yum2565) srbc-42(yum2566) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1252 Species: C. elegans Genotype: t10h9.1(yum2567) zc443.7(yum2568) y57a10c.10(yum2569) w05h5.1(yum2570) y57a10c.8(yum2571) f38h12.5(yum2572) y57a10c.9(yum2573) dct-12(yum2574) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1253 Species: C. elegans Genotype: srw-99(yum2575) srw-100(yum2576) srw-101(yum2577) srw-102(yum2578) srw-103(yum2579) srw-107(yum2580) srw-109(yum2581) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1254 Species: C. elegans Genotype: str-119(yum2582) str-121(yum2583) y45g12c.10(yum2585) str-120(yum2586) y45g12c.6(yum2587) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1255 Species: C. elegans Genotype: str-217(yum2588) c55a1.1(yum2589) str-220(yum2590) str-221(yum2591) str-222(yum2592) str-225(yum2594) str-226(yum2595) str-227(yum2596) str-228(yum2597) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1256 Species: C. elegans Genotype: dop-1(yum2598) dop-2(yum2599) dop-3(yum2600) dop-4(yum2601) dop-5(yum2602) c24a8.6(yum2603) dop-6(yum2604) ser-6(yum2605) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1257 Species: C. elegans Genotype: srg-25(yum2606) srg-26(yum2607) srg-27(yum2607) srg-28(yum2608) srg-29(yum2609) srg-30(yum2610) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1258 Species: C. elegans Genotype: str-27(yum2611) str-28(yum2612) str-30(yum2613) str-31(yum2614) str-33(yum2615) str-35(yum2616) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1259 Species: C. elegans Genotype: srh-288(yum2617) srh-289(yum2618) srh-290(yum2619) srh-286(yum2620) srh-287(yum2621) srh-291(yum2622) srh-292(yum2623) srh-293(yum2624) srh-295(yum2625) srh-296(yum2626) srh-297(yum2627) srh-298(yum2628) srh-299(yum2629) srh-300(yum2630) srh-301(yum2631) srh-303(yum2632) srh-304(yum2633) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1260 Species: C. elegans Genotype: srw-9(yum2634) srw-10(yum2635) srw-11(yum2636) srw-12(yum2637) srw-13(yum2638) srw-14(yum2639) srw-15(yum2640) III. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1261 Species: C. elegans Genotype: c15h9.3(yum2641) r13a5.15(yum2642) r11f4.2(yum2643) k01a12.3(yum2644) t22c8.1(yum2645) r02d5.6(yum2646) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1262 Species: C. elegans Genotype: srt-42(yum2647) srt-43(yum2648) srt-44(yum2649) srt-45(yum2650) srt-47(yum2651) srt-48(yum2652) srt-49(yum2653) srt-50(yum2654) srt-52(yum2655) srt-53(yum2656) y57g11c.28(yum2657) y57g11c.30(yum2658) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1263 Species: C. elegans Genotype: srbc-29(yum2659) srbc-30(yum2660) srbc-31(yum2661) srbc-32(yum2662) srbc-33(yum2663) srbc-34(yum2664) srbc-36(yum2665) srbc-37(yum2666) srbc-38(yum2667) srbc-39(yum2668) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1264 Species: C. elegans Genotype: sri-39(yum1035) sri-50(yum1036) sri-51(yum1037) sri-53(yum1038) y27f2a.10(yum1039) zk105.8(yum1040) y27f2a.11(yum1041) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1265 Species: C. elegans Genotype: str-3(yum1042) str-4(yum2669) str-5(yum2670) str-1(yum2671) str-6(yum2672) str-7(yum2673) str-9(yum2674) c04c3.7(yum2675) str-8(yum2676) t23d5.8(yum2677) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1266 Species: C. elegans Genotype: c31b8.1(yum2861) y70c5a.2(yum2862) w02h5.16(yum2863) w02h5.11(yum2864) f18e9.8(yum2865) m03f8.7(yum2866) y70c5a.4(yum2867) m01d7.9(yum2868) w06g6.15(yum2869) y70c5b.2(yum2870) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1267 Species: C. elegans Genotype: tag-293(yum2678) c29f9.9(yum2679) c39b10.1(yum2680) c44b9.2(yum2681) zc132.9(yum2682) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1268 Species: C. elegans Genotype: srh-113(yum2683) srh-115(yum2684) srh-116(yum2685) srh-118(yum2686) srh-119(yum2687) srh-120(yum2688) srh-122(yum2689) srh-123(yum2690) srh-95(yum2691) srh-97(yum2692) srh-99(yum2693) srh-100(yum2694) srh-102(yum2695) srh-104(yum2696) srh-105(yum2697) srh-109(yum2698) srh-111(yum2699) srh-112(yum2700) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1269 Species: C. elegans Genotype: srb-1(yum2701) srb-2(yum2702) srb-3(yum2703) srb-5(yum2704) srb-12(yum2705) srb-13(yum2706) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1270 Species: C. elegans Genotype: sri-57(yum2707) sri-61(yum2708) sri-62(yum2709) sri-63(yum2710) sri-65(yum2711) sri-78(yum2712) sri-54(yum2713) sri-60(yum2714) y27f2a.11(yum2715) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1271 Species: C. elegans Genotype: srh-166(yum2716) srh-167(yum2717) srh-169(yum2718) srh-146(yum2719) srh-147(yum2720) srh-148(yum2721) srh-149(yum2722) srh-154(yum2723) srh-159(yum2724) srh-170(yum2725) srh-171(yum2726) srh-172(yum2727) srh-173(yum2728) srh-174(yum2729) srh-177(yum2730) srh-178(yum2731) srh-179(yum2732) srh-180(yum2733) srh-181(yum2734) srh-182(yum2735) srh-183(yum2736) srh-184(yum2737) srh-308(yum2738) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1272 Species: C. elegans Genotype: srbc-1(yum2739) srbc-2(yum2740) srbc-3(yum2741) srbc-5(yum2742) srbc-6(yum2743) srbc-7(yum2744) srbc-8(yum2745) srbc-9(yum2746) srbc-10(yum2747) srbc-11(yum2748) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1273 Species: C. elegans Genotype: srh-74(yum2749) srh-75(yum2750) srh-76(yum2751) srh-78(yum2752) srh-79(yum2753) srh-80(yum2754) srh-82(yum2755) srh-83(yum2756) srh-85(yum2757) srh-87(yum2758) srh-88(yum2759) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1274 Species: C. elegans Genotype: srh-24(yum2760) srh-25(yum2761) srh-27(yum2762) srh-28(yum2763) srh-30(yum2764) srh-31(yum2765) srh-32(yum2766) srh-46(yum2767) srh-47(yum2768) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1275 Species: C. elegans Genotype: mgl-1(yum2769) mgl-2(yum2770) mgl-3(yum2771) c30a5.10(yum2772) f35h10.10(yum2773) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1276 Species: C. elegans Genotype: str-123(yum2774) str-124(yum2775) str-125(yum2776) str-129(yum2777) str-130(yum2778) str-131(yum2779) str-134(yum2780) str-135(yum2781) str-136(yum2782) str-145(yum2783) t06c12.11(yum2784) X. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1277 Species: C. elegans Genotype: srt-27(yum2785) srt-28(yum2786) srt-29(yum2787) srt-30(yum2788) srt-31(yum2789) srt-32(yum2790) srt-33(yum2791) srt-34(yum2792) srt-74(yum2793) srt-35(yum2794) srt-36(yum2795) srt-37(yum2796) srt-38(yum2797) srt-39(yum2798) srt-40(yum2799) srt-41(yum2800) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1278 Species: C. elegans Genotype: str-63(yum2801) str-64(yum2802) str-66(yum2803) str-61(yum2804) str-67(yum2805) str-68(yum2806) str-69(yum2807) c41g6.12(yum2808) str-52(yum2810) str-60(yum2811) str-71(yum2812) str-73(yum2813) str-74(yum2814) str-77(yum2815) f22f7.12(yum2816) str-76(yum2817) str-265(yum2818) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1590 Species: C. elegans Genotype: c27h5.6(yum2827) c50e3.7(yum2828) y75b8a.34(yum2829) c50e3.9(yum2830) f42a6.1(yum2831) y55h10a.2(yum2832) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1591 Species: C. elegans Genotype: f54e2.5(yum2833) c34b4.5(yum2834) f54e2.9(yum2835) f57b1.1(yum2836) f57b1.9(yum2837) f58f6.5(yum2838) f58f6.6(yum2839) t09e8.4(yum2840) y51a2b.2(yum2841) zc196.8(yum2842) zc196.9(yum2843) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1592 Species: C. elegans Genotype: h20j04.7(yum2844) k03h6.4(yum2845) r13h4.7(yum2846) c34b4.3(yum2847) t23b3.6(yum2848) y26g10.1(yum2849) y26g10.4(yum2850) II. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1680 Species: C. elegans Genotype: srtx-1(yum2906) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1681 Species: C. elegans Genotype: tpra-1(yum2907) IV. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CHS1713 Species: C. elegans Genotype: glb-33(yum2917) V. Description: Engineered null mutations in predicted GPCR genes. Reference: Pu L, et al. Nat Commun. 2023 Dec 18;14(1):8410. PMID: 38110404. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: CCHEN group Received: 11/20/24 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: CL183 Species: Caenorhabditis elegans Genotype: him-5(e1490) srf-4(ct109) V. Description: Animals commonly have a protruding vulva. Unc (slow moving and non-sinusoidal body posture). Egl. Ectopic surface binding of the lectins WGA and SBA. Males are infertile and Mab-crumpled spicules and abnormal rays and lack diagonal sex muscles. Mutagen: EMS Outcrossed: x10 Made by: Link C Received: 12/11/89 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CL2006 Species: Caenorhabditis elegans Genotype: dvIs2. Description: dvIs2 [pCL12(unc-54/human Abeta peptide 1-42 minigene) + rol-6(su1006)]. Temperature-sensitive. Maintain at 15C. Adult onset paralysis and egg-laying deficiency when raised at 20C. A few animals will be paralyzed even at permissive temperatures. Received new stock from CL 8/2005. Mutagen: Outcrossed: x3 Made by: Received: 03/25/03 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CL2008 Species: Caenorhabditis elegans Genotype: him-5 (e1490) V; dvIs3. Description: dvIs3 [unc-54p::human transthyretin + rol-6(su1006)]. Rollers. Him. Expression of wild-type human transthyretin (TTR) in body wall muscles. Transthyretin is secreted from the muscles into the pseudocoelom and concentrates in the coelomocytes. Reference: Link CD (1995) Proc Natl Acad Sci U S A 92:9368-9372. Mutagen: Gamma radiation Outcrossed: x0 Made by: Chris Link Received: 12/16/14 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CL2070 Species: Caenorhabditis elegans Genotype: dvIs70. Description: dvIs70 [hsp-16.2p::GFP + rol-6(su1006)]. Roller. Integrated array containg pCL25 (hsp-16-2 promoter/GFP transcriptional fusion) and pRF4. Shows robust induction of GFP expression after heat induction. Not known where dvIs70 is integrated. Mutagen: Outcrossed: x9 Made by: Chris Link Received: 09/29/00 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CL208 Species: Caenorhabditis elegans Genotype: srf-9(dv4) him-5(e1490) V. Description: Animals are somewhat smaller than WT and commonly have a protruding vulva. Unc(slow moving and non-sinusoidal body posture). Egl. Ectopic surface binding of the lectins WGA and SBA. Males are infertile and Mab. Mutagen: EMS Outcrossed: x5 Made by: Chris Link Received: 10/05/92 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CL2120 Species: Caenorhabditis elegans Genotype: dvIs14. Description: dvIs14 [(pCL12) unc-54::beta 1-42 + (pCL26) mtl-2::GFP]. mtl-2::GFP produces strong constitutive intestinal expression of GFP at all developmental stages. Expresses human AB peptide and accumulates B-amyloid fibrils. AB toxicity enhanced at higher temperatures. Mutagen: Outcrossed: x3 Made by: Received: 08/06/08 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CL2122 Species: Caenorhabditis elegans Genotype: dvIs15. Description: dvIs15 [(pPD30.38) unc-54(vector) + (pCL26) mtl-2::GFP]. Control strain for CL2120. Phenotype apparently WT. Mutagen: Outcrossed: x0 Made by: Received: 08/06/08 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CL2166 Species: Caenorhabditis elegans Genotype: dvIs19 III. Description: dvIs19 [(pAF15)gst-4p::GFP::NLS] III. Oxidative stress-inducible GFP. Mutagen: Outcrossed: x6 Made by: Received: 03/25/03 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CL2179 Species: Caenorhabditis elegans Genotype: smg-1(cc546) I; dvIs179. Description: dvIs179 [myo-3p::GFP::3' UTR(long) + rol-6(su1006)]. Maintain at 16C. Superficially wild-type Roller; expression of GFP in body wall muscles increases with temperature. References: Link CD, et al. (2006) J Biol Chem. Jan 20;281(3):1808-16. Hassan WM, et al. (2014) Neurobiology of Aging. [NOTE: The temperature-sensitive allele cc546 causes an M1957L change in SMG-1. The lesion is an atg>ttg transversion in exon 35. Flanking sequences follow with the mutation site indicated with a capital A: ttggtggtcggttacaaaacgatattcaaga tcactggcagtcatgagtAtggttggatcagttttaggactcggtgatcg acatttggacaatttattg The lesion is detectable via SNP-snip with the mutation causing loss of an MslI site. Primers are for a 323 bp product. Digest with MslI to 86+237 in the wild type, uncut as 323 in the mutant. DJR701(f): CAGTCGTGAGCTTTGGATGCGTGC DJR702(r): TCGGGGATACGCAGATTCTTTCCC. Pedone ... Reiner G3 (2021).] Mutagen: Gamma radiation Outcrossed: x0 Made by: Chris Link Received: 12/16/14 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CL2331 Species: Caenorhabditis elegans Genotype: dvIs37. Description: dvIs37 [myo-3p::GFP::A-Beta (3-42) + rol-6(su1006)]. Maintain at 16C. Roller. Diffuse and aggregated GFP expression in body wall muscle. Low brood size. Sicker at higher temperatures (e.g. 25C). Reference: Link CD, et al. (2008) Neurobiology of Disease,32(3):420-5. Mutagen: Gamma radiation Outcrossed: x1 Made by: Chris Link Received: 12/16/14 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CL2337 Species: Caenorhabditis elegans Genotype: smg-1(cc546) I; dvIs38. Description: dvIs38 [myo-3p::GFP::degron::3' UTR(long) + rol-6(su1006)]. Maintain at 16C. Rollers. Temperature-dependent expression of aggregating GFP in body wall muscle (weak at 16C, strong at 25C). Animals become paralyzed if upshifted as larvae to 25C due to expression of aggregating GFP. References: Link CD, et al. (2006) J Biol Chem. Jan 20;281(3):1808-16. Hassan WM, et al. (2014) Neurobiology of Aging. [NOTE: The temperature-sensitive allele cc546 causes an M1957L change in SMG-1. The lesion is an atg>ttg transversion in exon 35. Flanking sequences follow with the mutation site indicated with a capital A: ttggtggtcggttacaaaacgatattcaaga tcactggcagtcatgagtAtggttggatcagttttaggactcggtgatcg acatttggacaatttattg The lesion is detectable via SNP-snip with the mutation causing loss of an MslI site. Primers are for a 323 bp product. Digest with MslI to 86+237 in the wild type, uncut as 323 in the mutant. DJR701(f): CAGTCGTGAGCTTTGGATGCGTGC DJR702(r): TCGGGGATACGCAGATTCTTTCCC. Pedone ... Reiner G3 (2021).] Mutagen: Gamma radiation Outcrossed: x0 Made by: Chris Link Received: 12/16/14 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CL2355 Species: Caenorhabditis elegans Genotype: smg-1(cc546) dvIs50 I. Description: dvIs50 [pCL45 (snb-1::Abeta 1-42::3' UTR(long) + mtl-2::GFP] I. Maintain at 16C. Pan-neuronal expresion of human Abeta peptide. Constitutive intestinal expression of GFP from marker transgene. Strain shows deficits in chemotaxis, associative learning, and thrashing in liquid. Strain also has incomplete sterility due to germline proliferation defects and embryonic lethality. Maintain at 16 C to reduce selection against transgene, although this does not alter the partial sterility. Reference: Wu Y., et al. J Neurosci. 2006 Dec 13;26(50):13102-13. [NOTE: The temperature-sensitive allele cc546 causes an M1957L change in SMG-1. The lesion is an atg>ttg transversion in exon 35. Flanking sequences follow with the mutation site indicated with a capital A: ttggtggtcggttacaaaacgatattcaaga tcactggcagtcatgagtAtggttggatcagttttaggactcggtgatcg acatttggacaatttattg The lesion is detectable via SNP-snip with the mutation causing loss of an MslI site. Primers are for a 323 bp product. Digest with MslI to 86+237 in the wild type, uncut as 323 in the mutant. DJR701(f): CAGTCGTGAGCTTTGGATGCGTGC DJR702(r): TCGGGGATACGCAGATTCTTTCCC. Pedone ... Reiner G3 (2021).] Mutagen: gamma irradiation Outcrossed: x3 Made by: Chris Link Received: 01/05/12 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CL261 Species: Caenorhabditis elegans Genotype: him-5(e1490) V; srf-5(ct115) X. Description: WT except for ectopic surface binding of wheat germ agglutinin (WGA). Mutagen: EMS Outcrossed: x10 Made by: Link C Received: 08/13/90 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CL2621 Species: Caenorhabditis elegans Genotype: smg-1(cc546) I; dvIs75. Description: dvIs75 [myo-3::Abeta 1-42 G37L::3' UTR(long) + mtl-2::GFP)]. Temperature-inducible induction of human Abeta peptide in body wall muscle; paralysis in ~32 hr if induced as L3 larvae. Maintain at 16 C to prevent strong Abeta induction and larval paralysis/arrest. Reference: Fonte V., et al. Mol Neurodegener. 2011 Aug 23;6(1):61. [NOTE: The temperature-sensitive allele cc546 causes an M1957L change in SMG-1. The lesion is an atg>ttg transversion in exon 35. Flanking sequences follow with the mutation site indicated with a capital A: ttggtggtcggttacaaaacgatattcaaga tcactggcagtcatgagtAtggttggatcagttttaggactcggtgatcg acatttggacaatttattg The lesion is detectable via SNP-snip with the mutation causing loss of an MslI site. Primers are for a 323 bp product. Digest with MslI to 86+237 in the wild type, uncut as 323 in the mutant. DJR701(f): CAGTCGTGAGCTTTGGATGCGTGC DJR702(r): TCGGGGATACGCAGATTCTTTCCC. Pedone ... Reiner G3 (2021).] Mutagen: UV irradiation Outcrossed: x0 Made by: Chris Link Received: 01/05/12 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CL264 Species: Caenorhabditis elegans Genotype: srf-8(dv38) V. Description: Animals are somewhat smaller than WT. Often have protuding vulva. Unc(slow moving and non-sinusoidal body posture). Egl. Males are infertile and Mab. Ectopic surface binding of lectins WGA and SBA. Mutagen: EMS Outcrossed: x10 Made by: Chris Link Received: 10/05/92 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CL2659 Species: Caenorhabditis elegans Genotype: smg-1(cc546) I; dvIs770. Description: dvIs770 [myo-3::Abeta 1-42 wt::3' UTR(long) + mtl-2::GFP]. Maintain at 16 C to prevent strong Abeta induction and larval paralysis/arrest. Temperature-inducible induction of human Abeta peptide in body wall muscle; paralysis in 18-24 hr if induced as L3 larvae. NOTE: dvIs770 was originally described as dvIs70 in Fonte et al, 2011. The name of this array was changed to dvIs770 to avoid confusion with dvIs70 [hsp-16.2p::GFP + rol-6(su1006)] carried in strain CL2070. Reference: Fonte V., et al. Mol Neurodegener. 2011 Aug 23;6(1):61. [NOTE: The temperature-sensitive allele cc546 causes an M1957L change in SMG-1. The lesion is an atg>ttg transversion in exon 35. Flanking sequences follow with the mutation site indicated with a capital A: ttggtggtcggttacaaaacgatattcaaga tcactggcagtcatgagtAtggttggatcagttttaggactcggtgatcg acatttggacaatttattg The lesion is detectable via SNP-snip with the mutation causing loss of an MslI site. Primers are for a 323 bp product. Digest with MslI to 86+237 in the wild type, uncut as 323 in the mutant. DJR701(f): CAGTCGTGAGCTTTGGATGCGTGC DJR702(r): TCGGGGATACGCAGATTCTTTCCC. Pedone ... Reiner G3 (2021).] Mutagen: UV irradiation Outcrossed: x0 Made by: Chris Link Received: 01/05/12 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CL4176 Species: Caenorhabditis elegans Genotype: smg-1(cc546) I; dvIs27 X. Description: dvIs27 [myo-3p::A-Beta (1-42)::let-851 3'UTR) + rol-6(su1006)] X. Rollers. Temperature sensitive: needs to be propagated at 15C. Upshift larval animals to check that the worms get paralyzed and give offspring that arrest as eggs/early larvae. This strain produces low levels of beta amyloid peptide even when grown at low temperature, and therefore there is always some selection for loss of transgene copies. It is recommended to maintain growing stock plates at 15-16 degrees C by transferring small numbers of animals each generation rather than by "chunking", which increases the effective population size and therefore the chance of a relatively rare transgene loss, and then this revertant taking over the population. The strain should also be frozen shortly after being received. This strain can only be sent to academic users and not to commercial organizations. [NOTE: The temperature-sensitive allele cc546 causes an M1957L change in SMG-1. The lesion is an atg>ttg transversion in exon 35. Flanking sequences follow with the mutation site indicated with a capital A: ttggtggtcggttacaaaacgatattcaaga tcactggcagtcatgagtAtggttggatcagttttaggactcggtgatcg acatttggacaatttattg The lesion is detectable via SNP-snip with the mutation causing loss of an MslI site. Primers are for a 323 bp product. Digest with MslI to 86+237 in the wild type, uncut as 323 in the mutant. DJR701(f): CAGTCGTGAGCTTTGGATGCGTGC DJR702(r): TCGGGGATACGCAGATTCTTTCCC. Pedone ... Reiner G3 (2021).] Mutagen: Outcrossed: x3 Made by: Chris Link Received: 05/10/07 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CL6049 Species: Caenorhabditis elegans Genotype: dvIs62 X. Description: dvIs62 [snb-1p::hTDP-43/3' long UTR + mtl-2p::GFP] X. Temperature-sensitive. Maintain at 16 to minimize selection against transgene. Uncoordinated from hatching; phenotype is stronger at higher temperatures. Intestinal GFP expression. Reference: Ash PE, et al. Hum Mol Genet. 2010 Aug 15;19(16):3206-18. Mutagen: UV/TMP Outcrossed: x3 Made by: Chris Link Received: 02/13/15 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CL6180 Species: Caenorhabditis elegans Genotype: smg-1(cc546) I; dvIs19 III; skn-1(zu67)/nT1 [unc-?(n754) let-?] (IV;V); dvIs27 X. Description: dvIs19 [(pAF15) gst-4p::GFP::NLS] III. dvIs27 [myo-3p::A-Beta (1-42)::let-851 3'UTR) + rol-6(su1006)] X. Roller with weak constitutive GFP expression. Balanced strain, segregates Rol Uncs [skn-1(zu67) heterozygotes], Rol nonUncs [skn-1(zu67) homozygotes] and dead eggs. Maintain by picking Rol Uncs. Paralyzed if upshifted as larvae to 25C. References: Dostal, V and Link CD (2010) J Vis Exp. Oct 9;(44). Dostal V, Roberts CM, Link CD (2010) Genetics Nov;186(3):857-66. [NOTE: The temperature-sensitive allele cc546 causes an M1957L change in SMG-1. The lesion is an atg>ttg transversion in exon 35. Flanking sequences follow with the mutation site indicated with a capital A: ttggtggtcggttacaaaacgatattcaaga tcactggcagtcatgagtAtggttggatcagttttaggactcggtgatcg acatttggacaatttattg The lesion is detectable via SNP-snip with the mutation causing loss of an MslI site. Primers are for a 323 bp product. Digest with MslI to 86+237 in the wild type, uncut as 323 in the mutant. DJR701(f): CAGTCGTGAGCTTTGGATGCGTGC DJR702(r): TCGGGGATACGCAGATTCTTTCCC. Pedone ... Reiner G3 (2021).] Mutagen: Gamma radiation Outcrossed: x0 Made by: Chris Link Received: 12/16/14 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CL691 Species: Caenorhabditis elegans Genotype: dvIs19 III; skn-1(zu67) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: dvIs19 [(pAF15) gst-4p::GFP::NLS] III. Oxidative stress-inducible GFP. Segregates Unc skn-1(zu67) heterozygotes, arrested eggs/larvae (nT1 homozygotes), and wild type skn-1(zu67) homozygotes (sterile). All genotypes show constitutive weak GFP expression. Upon exposure to SKN-1 inducers (e.g., azide), strong induction of GFP is observered in skn-1/+ hets; there is no induction in skn-1 homozygotes. Pick Uncs to maintain -- although this strain is nominally balanced, nT1 can break down. Reference: Dostal, V., et al. Genetics. 2010 Nov;186(3):857-66. Mutagen: gamma irradiation Outcrossed: x0 Made by: Chris Link Received: 01/05/12 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CL802 Species: Caenorhabditis elegans Genotype: smg-1(cc546) I; rol-6(su1006) II. Description: Rollers. Maintain under normal conditions. Standard control for CL4176; originally used CL1175 as the control, but subsequently it was found that CL1175 can produce some A-Beta. Reference: Fonte V., et al. Mol Neurodegener. 2011 Aug 23;6(1):61. [NOTE: The temperature-sensitive allele cc546 causes an M1957L change in SMG-1. The lesion is an atg>ttg transversion in exon 35. Flanking sequences follow with the mutation site indicated with a capital A: ttggtggtcggttacaaaacgatattcaaga tcactggcagtcatgagtAtggttggatcagttttaggactcggtgatcg acatttggacaatttattg The lesion is detectable via SNP-snip with the mutation causing loss of an MslI site. Primers are for a 323 bp product. Digest with MslI to 86+237 in the wild type, uncut as 323 in the mutant. DJR701(f): CAGTCGTGAGCTTTGGATGCGTGC DJR702(r): TCGGGGATACGCAGATTCTTTCCC. Pedone ... Reiner G3 (2021).] Mutagen: Outcrossed: x0 Made by: Wail Hassan Received: 05/10/07 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CLP1360 Species: Caenorhabditis elegans Genotype: pmp-4(twn16) IV. Description: twn16 is a 1651 bp deletion removing 1027 bp of the promoter sequence, the transcriptional start site, and the first two exons of pmp-4. Superficially wild-type. Normal dauer formation. Reference: Tsai SH, et al. Cell Rep. 2024 Mar 22;43(4):113996. doi: 10.1016/j.celrep.2024.113996. PMID: 38520690. Mutagen: Outcrossed: x3 Made by: Shang-Heng Tsai Received: 05/06/24 National Taiwan University School of Medicine, Taiwan ----------------------------------------------------------------------------- Strain: CLP1445 Species: Caenorhabditis elegans Genotype: pmp-4(twn16) IV; twnEx656. Description: twnEx656 [pmp-4p::pmp-4 + elt-2p::GFP]. Pick GFP+ animals to maintain. Superficially wild-type. twnEx656 contains 1.2 kb pmp-4 promoter driving expression of 2.2 kb pmp-4 cDNA; transgene rescues behavioral phenotype of twn16 mutants. twn16 is a 1651 bp deletion removing 1027 bp of the promoter sequence, the transcriptional start site, and the first two exons of pmp-4. twn16 has been out-crossed 3 times in this strain. Reference: Tsai SH, et al. Cell Rep. 2024 Mar 22;43(4):113996. doi: 10.1016/j.celrep.2024.113996. PMID: 38520690. Mutagen: Outcrossed: x3 Made by: Shang-Heng Tsai Received: 05/06/24 National Taiwan University School of Medicine, Taiwan ----------------------------------------------------------------------------- Strain: CLP215 Species: C. elegans Genotype: twnEx8. Description: twnEx8 (mec-7p::tomm20::mCherry + myo-2p::GFP). Punctate mCherry signals denote mitochondria in six touch neurons. Pick animals GFP+ in the pharynx to maintain. Reference: Jiang HC, et al. Proc Natl Acad Sci U S A. 2015 Jul 14;112(28):8768-73. Mutagen: Outcrossed: x0 Made by: Chiu-Ying Peng Received: 03/13/19 National Taiwan University School of Medicine, Taiwan ----------------------------------------------------------------------------- Strain: CLP546 Species: C. elegans Genotype: twnEx185. Description: twnEx185 [mec-7p::MTS::roGFP + mec-7p::TOMM20::mCherry + ttx-3p::GFP]. Pick animals with GFP expression in head neurons (AIY) to maintain. roGFP can be used to monitor redox status of the mitochondrial matrix in the six touch receptor neurons: the oxidation-reduction status of mitochondrial matrix can be monitored with roGFP, a GFP variant that increases brightness in a more oxidized environment. The mitochondrial outer membrane in these neurons are marked by mCherry. Reference: Jiang HC, et al. Proc Natl Acad Sci USA. 2015 Jul 14;112(28):8768-73. doi: 10.1073/pnas.1501831112. PMID: 26124107. [NOTE: twnEx185 is incorrectly described as carrying myo-2p::GFP in the reference publication. The correct co-injection marker is ttx-3::GFP.] Mutagen: Outcrossed: x0 Made by: Hao-Ching Jiang Received: 11/08/23 National Taiwan University School of Medicine, Taiwan ----------------------------------------------------------------------------- Strain: COP1622 Species: C. elegans Genotype: nas-38(knu568) X. Description: Increased movement quiescence during lethargus. knu568 is a specific in-frame deletion of the TSP1 domain. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Knudra Transgenics Received: 03/22/21 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: COP1626 Species: Caenorhabditis elegans Genotype: ins-34(knu572) IV. Description: F52B11.6. Superficially wild-type. knu572 is an F125L point mutation mimicking human mutation F119L in patients with PMM2 deficiency disease. Strain is sensitive to bortezomib (proteasome blocker) and displays larval arrest in liquid culture. This strain may not be distributed to commercial or for-profit entities. Please contact ethan@perlara.com for more information. Mutagen: Outcrossed: x0 Made by: NemaMetrix Received: 03/13/19 Perlstein Lab, San Francisco, CA ----------------------------------------------------------------------------- Strain: COP1635 Species: C. elegans Genotype: nas-38(knu579 ok3407) X. Description: Specific in-frame deletion of the astacin domain in ok3407 background suppresses increased quiescence from the ok3407 allele. Quiescence behavior in this strain is reverted to wild-type. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Knudra Transgenics Received: 03/22/21 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: COP1863 Species: Caenorhabditis elegans Genotype: goa-1(knu751) I. Description: Hyperactive, protruding vulva. knu751 is an A221D point mutation associated with GNAO1-associated epileptic encephalopathy. This strain may not be distributed to commercial or for-profit entities. Please contact ethan@perlara.com for more information. Mutagen: Outcrossed: x0 Made by: NemaMetrix Received: 03/13/19 Perlstein Lab, San Francisco, CA ----------------------------------------------------------------------------- Strain: COP1883 Species: C. elegans Genotype: unc-68(knu769) V. Description: The UNC-68a R2246H missense mutation (knu769) corresponds to a human myopathic variant, RyR1:p.R2163H. Subtle effects on locomotion, and altered response to halothane and aldicarb. Reference: Graham B, et al. Front. Genet. 2020; 11:37. doi: 10.3389/fgene.2020.00037 PMID: 32174957 Mutagen: Outcrossed: x0 Made by: Brittany Graham Received: 10/23/20 University of Leeds, Leeds, England ----------------------------------------------------------------------------- Strain: COP1918 Species: Caenorhabditis elegans Genotype: rskn-1(knu796) I. Description: Superficially wild-type. knu796 is a K216E point mutation mimicking a patient allele associated with Coffin-Lowry syndrome. This strain may not be distributed to commercial or for-profit entities. Please contact ethan@perlara.com for more information. Mutagen: Outcrossed: x0 Made by: NemaMetrix Received: 03/19/16 Perlstein Lab, San Francisco, CA ----------------------------------------------------------------------------- Strain: COP1932 Species: C. elegans Genotype: unc-68(knu810) V. Description: The UNC-68a K3675Q missense mutation (knu810) corresponds to a human myopathic variant, RyR1:p.K3452Q. Subtle effects on locomotion, and altered response to halothane and aldicarb. Reference: Graham B, et al. Front. Genet. 2020; 11:37. doi: 10.3389/fgene.2020.00037 PMID: 32174957 Mutagen: Outcrossed: x0 Made by: Brittany Graham Received: 10/23/20 University of Leeds, Leeds, England ----------------------------------------------------------------------------- Strain: COP1944 Species: C. elegans Genotype: unc-68(knu822) V. Description: The UNC-68a R2564H missense mutation (knu822) corresponds to a human myopathic variant, RyR1:p.R2458H. Subtle effects on locomotion, and altered response to halothane and aldicarb. Reference: Graham B, et al. Front. Genet. 2020; 11:37. doi: 10.3389/fgene.2020.00037 PMID: 32174957 Mutagen: Outcrossed: x0 Made by: Brittany Graham Received: 10/23/20 University of Leeds, Leeds, England ----------------------------------------------------------------------------- Strain: COP1947 Species: C. elegans Genotype: unc-68(knu825) V. Description: The UNC-68a R2560H missense mutation (knu825) corresponds to a human myopathic variant, RyR1:p.R2454H. Subtle effects on locomotion, and altered response to halothane and aldicarb. Reference: Graham B, et al. Front. Genet. 2020; 11:37. doi: 10.3389/fgene.2020.00037 PMID: 32174957 Mutagen: Outcrossed: x0 Made by: Brittany Graham Received: 10/23/20 University of Leeds, Leeds, England ----------------------------------------------------------------------------- Strain: COP1950 Species: C. elegans Genotype: unc-68(knu828) V. Description: The UNC-68a R5021H missense mutation (knu769) corresponds to a human myopathic variant, RyR1:p.R4861H. Subtle effects on locomotion, and altered response to halothane and aldicarb. Reference: Graham B, et al. Front. Genet. 2020; 11:37. doi: 10.3389/fgene.2020.00037 PMID: 32174957 Mutagen: Outcrossed: x0 Made by: Brittany Graham Received: 10/23/20 University of Leeds, Leeds, England ----------------------------------------------------------------------------- Strain: COP2002 Species: Caenorhabditis elegans Genotype: K09E4.4(knu858) II. Description: knu858 is a Y146C point mutation representative of a patient mutation associated with MPSIIIB disease. This strain may not be distributed to commercial or for-profit entities. Please contact ethan@perlara.com for more information. Mutagen: Outcrossed: x0 Made by: NemaMetrix Received: 03/13/19 Perlstein Lab, San Francisco, CA ----------------------------------------------------------------------------- Strain: COP2004 Species: Caenorhabditis elegans Genotype: agl-1(knu860) II. Description: Superficially wild-type. knu860 is a W1044X point mutation associated with Cori Disease. This strain may not be distributed to commercial or for-profit entities. Please contact ethan@perlara.com for more information. Mutagen: Outcrossed: x0 Made by: NemaMetrix Received: 03/13/19 Perlstein Lab, San Francisco, CA ----------------------------------------------------------------------------- Strain: COP2008 Species: Caenorhabditis elegans Genotype: agl-1(knu864) II. Description: Superficially wild-type. knu864 is a S1444R point mutation associated with Cori Disease. This strain may not be distributed to commercial or for-profit entities. Please contact ethan@perlara.com for more information. Mutagen: Outcrossed: x0 Made by: NemaMetrix Received: 03/13/19 Perlstein Lab, San Francisco, CA ----------------------------------------------------------------------------- Strain: COP2012 Species: Caenorhabditis elegans Genotype: agl-1(knu867) II. Description: Superficially wild-type. knu867 is a 6955 bp deletion in agl-1. This strain may not be distributed to commercial or for-profit entities. Please contact ethan@perlara.com for more information.services/. For more information, please contact ethan@perlara.com Mutagen: Outcrossed: x0 Made by: NemaMetrix Received: 03/13/19 Perlstein Lab, San Francisco, CA ----------------------------------------------------------------------------- Strain: COP2014 Species: Caenorhabditis elegans Genotype: sul-1(knu869) X. Description: knu869 is a 4077 bp deletion in sul-1. This strain may not be distributed to commercial or for-profit entities. Please contact ethan@perlara.com for more information.services/. For more information, please contact ethan@perlara.com Mutagen: Outcrossed: x0 Made by: NemaMetrix Received: 03/13/19 Perlstein Lab, San Francisco, CA ----------------------------------------------------------------------------- Strain: COP2029 Species: C. elegans Genotype: unc-68(knu879) V. Description: The UNC-68a A5101T missense mutation (knu879) corresponds to a human myopathic variant, RyR1:p.A4940T. Mutagen: Outcrossed: x0 Made by: Brittany Graham Received: 10/23/20 University of Leeds, Leeds, England ----------------------------------------------------------------------------- Strain: COP227 Species: Caenorhabditis elegans Genotype: oaSi41 II; unc-119(ed3) III. Description: oaSi41 [par-5p::GFP::par-5::par-5 3' UTR.2(prespliced) + unc-119(+)] II. MOS single copy insertion of PAR-5 under control of the PAR-5 3'UTR.2 isoform exclusively. Reference: Mikl, M. and Cowan, CR. Cell Rep. 2014 Sep 11;8(5):1380-90. Mutagen: Outcrossed: x03 Made by: Martin Mikl Received: 03/15/15 InVivo Biosystems, Eugene, or ----------------------------------------------------------------------------- Strain: COP2331 Species: C. elegans Genotype: spin-1(knu1010[spin-1::mCherry::loxP::HygR::loxP]) V. Description: mCherry tag inserted at the C-terminus of the endogenous spin-1 locus via CRISPR/Cas9 engineering. This strain may not be distributed to commercial or for-profit entities without prior written permission from In Vivo Biosystems. Please contact support@invivobiosystems.com for more information. Reference: Villalobos TV, et al. Nat Aging. 2023 Sep;3(9):1091-1106. PMID: 37580394. Mutagen: Outcrossed: x0 Made by: In Vivo Biosystems Received: 07/24/24 Louisiana State University ----------------------------------------------------------------------------- Strain: COP2341 Species: C. elegans Genotype: spin-2(knu1018[spin-2::mCherry::loxP::HygR::loxP]) IV. Description: mCherry tag inserted at the C-terminus of the endogenous spin-2 locus via CRISPR/Cas9 engineering. This strain may not be distributed to commercial or for-profit entities without prior written permission from In Vivo Biosystems. Please contact support@invivobiosystems.com for more information. Reference: Villalobos TV, et al. Nat Aging. 2023 Sep;3(9):1091-1106. PMID: 37580394. Mutagen: Outcrossed: x0 Made by: In Vivo Biosystems Received: 07/24/24 Louisiana State University ----------------------------------------------------------------------------- Strain: COP2343 Species: C. elegans Genotype: spin-3(knu1020[spin-3::mCherry::loxP::HygR::loxP]) X. Description: mCherry tag inserted at the C-terminus of the endogenous spin-3 locus via CRISPR/Cas9 engineering. This strain may not be distributed to commercial or for-profit entities without prior written permission from In Vivo Biosystems. Please contact support@invivobiosystems.com for more information. Reference: Villalobos TV, et al. Nat Aging. 2023 Sep;3(9):1091-1106. PMID: 37580394. Mutagen: Outcrossed: x0 Made by: In Vivo Biosystems Received: 07/24/24 Louisiana State University ----------------------------------------------------------------------------- Strain: COP2412 Species: C. elegans Genotype: atg-9(ola511[delta AP]) V. Description: ola511 is aCRISPR-engineered allele deleting a conserved sorting motif in ATG-9, causing a 2- to 3-fold decrease in LGG-1-containing puncta (and therefore autophagosomes) in the AIY neurites. Reference: Yang S, et al. Neuron. 2022 Mar 2;110(5):824-840.e10. Mutagen: Outcrossed: x0 Made by: InVivo Biosystems Received: 09/02/22 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: COP2416 Species: C. elegans Genotype: spin-4(knu1071[spin-4::mCherry]) Ill. Description: mCherry tag inserted at the C-terminus of the endogenous spin-4 locus via CRISPR/Cas9 engineering. This strain may not be distributed to commercial or for-profit entities without prior written permission from In Vivo Biosystems. Please contact support@invivobiosystems.com for more information. Reference: Villalobos TV, et al. Nat Aging. 2023 Sep;3(9):1091-1106. PMID: 37580394. Mutagen: Outcrossed: x0 Made by: In Vivo Biosystems Received: 07/24/24 Louisiana State University ----------------------------------------------------------------------------- Strain: COP2456 Species: C. elegans Genotype: spin-4(knu1099) Ill. Description: knu1099 is an engineered 7437 bp deletion in spin-4. Flanking sequences immediately outside of the region deleted ares: 5′ flank (forward strand), 5′- GTT CGG TGG AGC GCG CCT GCG -3’; 3′ flank (reverse strand), 5′- GTC TGT GTT GCT GTT CCT CAT -3’. In between these flanking sequences, a three-frame stop as well as a unique primer-binding sequence were inserted in place of the deleted sequence. This strain may not be distributed to commercial or for-profit entities without prior written permission from In Vivo Biosystems. Please contact support@invivobiosystems.com for more information. Reference: Flora Y & Bohnert KA. Dev Biol. 2023 Dec:504:137-148. doi: 10.1016/j.ydbio.2023.09.013. PMID: 37805103. Mutagen: Outcrossed: x0 Made by: In Vivo Biosystems Received: 07/24/24 Louisiana State University ----------------------------------------------------------------------------- Strain: COP262 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; knuSi221. Description: knuSi221 [fib-1p::fib-1(genomic)::eGFP::fib-1 3' UTR + unc-119(+)]. Single copy insertion. fib-1 promoter, genomic sequence, and 3'UTR was inserted into pCFJ151 (ttTi5606) targeting vector and inserted into ttTi5605. Allen AK, Nesmith JE, and A Golden. 2014 G3:4(12)2329-43. Mutagen: Outcrossed: x0 Made by: Knudra Transgenics Received: 05/28/15 NIDDK/NIH ----------------------------------------------------------------------------- Strain: COP677 Species: Caenorhabditis elegans Genotype: ncr-1(knu4) X. Description: Cholesterol-sensitive. Smaller broods and sizes upon serial cholesterol deprivation. This strain may not be distributed to commercial or for-profit entities. Please contact ethan@perlara.com for more information. Mutagen: Outcrossed: x0 Made by: NemaMetrix Received: 03/13/19 Perlstein Lab, San Francisco, CA ----------------------------------------------------------------------------- Strain: CP101 Species: Caenorhabditis briggsae Genotype: Cbr-puf-2(nm66)/Cbr-dpy-?(nm4) II. Description: Larval-lethal puf-2 deletion allele. Heterozygotes are WT (slightly Dpy) and segregate 25% Dpy, 50% wild-type heterozygotes, and 25% larval lethal (arrest L1-L2). Maintain by picking WT and checking for correct segregation of progeny. Map distance between nm4 and nm66 has not been preciely determined, but is tight enough that >90% of non-Dpy non-Lva progeny from double-heterozygotes retain the parental genotype. Reference: Liu Q & Haag ES. J Exp Zool. 2013 Part B. Mutagen: Outcrossed: x Made by: Q. Liu Received: 08/20/13 University of Maryland, College Park, MD ----------------------------------------------------------------------------- Strain: CP157 Species: C. remanei Genotype: nmDf1 III. Description: nmDf1 removes all four tandem paralogs of the mss family (Cre-mss-1, Cre-mss-2, Cre-mss-3, and Cre-mss-4). Male sperm is less competitive than wild-type male sperm, and females have lower brood size due to inbreeding depression. Reference: Yin D, et al. Science. 2018 Jan 5;359(6371):55-61. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Da Yin Received: 06/21/18 University of Maryland, College Park, MD ----------------------------------------------------------------------------- Strain: CP158 Species: C. remanei Genotype: Cre-mss-1(nm74[HA:Cre-mss-1]) III. Description: The hemaglutinin (HA) epitope tag was inserted using CRISPR/Cas9 through homologous recombination. The nine amino acid HA epitope was placed between C. remanei (EM464) MSS-1 residues 22 and 23, one residue downstream of the predicted mature N-terminus after signal peptide cleavage. NOTE: nm74 was originally described as nmIs9. Derived from parental strain SB146. Reference: Yin D, et al. Science. 2018 Jan 5;359(6371):55-61. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Da Yin Received: 06/21/18 University of Maryland, College Park, MD ----------------------------------------------------------------------------- Strain: CP161 Species: C. briggsae Genotype: Cbr-unc-119(nm67) III; nmIs7. Description: nmIs7 [Cni-mss-1(+) + Cni-mss-2(+) + Cbr-myo-2::GFP + unc-119(+)]. Insertion site of transgene is not known, but it is not in LG III or X. Males with this transgene are more competitive in siring progeny; also a higher ratio of males in the population. Derived from parental strain CP99, which in turn was derived from AF16. Reference: Yin D, et al. Science. 2018 Jan 5;359(6371):55-61. Mutagen: Outcrossed: x1 Made by: Da Yin Received: 06/21/18 University of Maryland, College Park, MD ----------------------------------------------------------------------------- Strain: CP174 Species: Caenorhabditis briggsae Genotype: nmIs11. Description: nmIs11 [Cbr-msrp-3(+) + Cbr-unc-119(+) + myo-2::GFP]. GFP expression in pharynx. Wild-type (non-Unc) movement. Roughly two-fold over-expression of Cbr-msrp-3(+); has no measurable effect on fertility. Cbr-MSRP-3 is a sperm surface glycoprotein with homologs in C. elegans and other species. Reference: Van Goor J, et al. Curr Biol. 2025 35:1-7. Mutagen: Outcrossed: x2 Made by: Justin Van Goor Received: 03/23/25 University of Maryland, College Park, MD ----------------------------------------------------------------------------- Strain: CP175 Species: Caenorhabditis briggsae Genotype: nmIs12. Description: nmIs12 [Cbr-msrp-3(+) + Cbr-unc-119(+) + myo-2::GFP]. GFP expression in pharynx. Wild-type (non-Unc) movement. Roughly two-fold over-expression of Cbr-msrp-3(+); has no measurable effect on fertility. Cbr-MSRP-3 is a sperm surface glycoprotein with homologs in C. elegans and other species. Reference: Van Goor J, et al. Curr Biol. 2025 35:1-7. Mutagen: Microparticle Bombardment Outcrossed: x2 Made by: Justin Van Goor Received: 03/23/25 University of Maryland, College Park, MD ----------------------------------------------------------------------------- Strain: CP196 Species: Caenorhabditis briggsae Genotype: Cbr-msrp-3(nm85) I; mfIs29. Description: mfIs29 [Cel-lip-1::GFP + Cel-myo-2::GFP]. Strong GFP expression in pharynx and weak GFP expression in various somatic and germline tissues. nm85 is a likely null frameshift mutation in the sperm-expressed Cbr-msrp-3, but has no apparent reproductive phenotypes. To confirm presence of nm85 mutation, use primers AT19+AT20 (WT: 291 nt, nm85: 286 nt). AT19: AAGAAGAGAGAAACCAGAAGC. AT20: AAAAGTAAAACATACCGATCACA. Reference: Van Goor J, et al. Curr Biol. 2025 35:1-7. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Asan Turdiev Received: 03/23/25 University of Maryland, College Park, MD ----------------------------------------------------------------------------- Strain: CP198 Species: Caenorhabditis briggsae Genotype: Cbr-msrp-3(nm77[HA::Cbr-msrp-3]) I. Description: nm77 is an HA epitope tag inserted into the endogenous Cbr-msrp-3 locus, two codons after the predicted signal peptide cleavage site of Cbr-MSRP-3. Anti-HA antibodies recognize the tagged Cbr-MSRP-3 glycoprotein on immunoblots, in the membranous organelles of spermatids, and on the plasma membrane of activated sperm (including pseudopod) in both males and hermaphrodites. To confirm presence of the nm77 insertion, use primers AT19+AT20 (WT: 291 nt, nm77: 318 nt). AT19: AAGAAGAGAGAAACCAGAAGC. AT20: AAAAGTAAAACATACCGATCACA. Reference: Van Goor J, et al. Curr Biol. 2025 35:1-7. Mutagen: CRISPR-Cas9 Outcrossed: x2 Made by: Asan Turdiev Received: 03/23/25 University of Maryland, College Park, MD ----------------------------------------------------------------------------- Strain: CP201 Species: Caenorhabditis briggsae Genotype: nmDf3 I; mfIs29. Description: mfIs29 [Cel-lip-1::GFP + Cel-myo-2::GFP]. Strong GFP expression in pharynx and weak GFP expression in various somatic and germline tissues. nmDf3 is an 8411 bp deletion that removes Cbr-msrp-2, Cbr-msrp-3, Cbr-msrp-4, Cbr-msrp-6, and Cbr-msrp-6, but has no apparent reproductive phenotypes. [NOTE: Van Goor J, et al. (2005) incorrectly described the nmDf3 as a 5807 bp deletion.] To confirm presence of deletion, use primers AT72+AT73 (WT: 11213 nt, nmDf3: 2802) and AT20+AT75 (WT: 855 nt, nmDf3: no product). AT72: GTACGACGGATAGAGTGTGAT. AT73: CTGTGGGATTATGAAAAGACTC. AT20: AAAAGTAAAACATACCGATCACA. AT75: CAGCAGCAACCTTAGAACAT. Reference: Van Goor J, et al. Curr Biol. 2025 35:1-7. Mutagen: CRIPSR/Cas9 Outcrossed: x3 Made by: Asan Turdiev Received: 03/23/25 University of Maryland, College Park, MD ----------------------------------------------------------------------------- Strain: CP217 Species: Caenorhabditis briggsae Genotype: Cbr-msrp-1(nm86) nmDf3 I; mfIs29. Description: mfIs29 [Cel-lip-1::GFP + Cel-myo-2::GFP]. Strong GFP expression in pharynx and weak GFP expression in various somatic and germline tissues. nmDf3 is an 8411 bp deletion that removes Cbr-msrp-2, Cbr-msrp-3, Cbr-msrp-4, Cbr-msrp-6, and Cbr-msrp-6. Removal of all six Cbr-msrp paralogs has no apparent reproductive phenotypes. [NOTE: Van Goor J, et al. (2005) incorrectly described the nmDf3 as a 5807 bp deletion.] To confirm presence of nm86 mutation, use primers AT130+AT131 (WT: 184 nt, nm86: 224 nt). AT130: CGAAATAATTGAACCTACCAAGA. AT131: CACTCTCTCTGACTGCAAACG. To confirm presence of deletion, use primers AT72+AT73 (WT: 11213 nt, nmDf3: 2802) and AT20+AT75 (WT: 855 nt, nmDf3: no product). AT72: GTACGACGGATAGAGTGTGAT. AT73: CTGTGGGATTATGAAAAGACTC. AT20: AAAAGTAAAACATACCGATCACA. AT75: CAGCAGCAACCTTAGAACAT. Reference: Van Goor J, et al. Curr Biol. 2025 35:1-7. Mutagen: CRISPR-Cas9 Outcrossed: x3 Made by: Asan Turdiev Received: 03/23/25 University of Maryland, College Park, MD ----------------------------------------------------------------------------- Strain: CP3 Species: Caenorhabditis briggsae Genotype: Cbr-dpy-?(nm3) X. Description: X-linked Dpy. Mutagen: Outcrossed: x Made by: Received: 02/28/06 McMaster University, Hamilton, Canada ----------------------------------------------------------------------------- Strain: CP36 Species: Caenorhabditis briggsae Genotype: Cbr-fem-2(nm27) III. Description: XX animals have no obvious phenotype: self-fertile with normal brood size. XO animals are self-fertile hermaphrodites with low brood size and some somatic gonad defects. Cbr-fem-2/+ XO animals show late-onset germline feminization. AF16 was the parental strain. Mutagen: EMS Outcrossed: x3 Made by: Robin Cook Hill Received: 06/26/06 University of Maryland, College Park, MD ----------------------------------------------------------------------------- Strain: CP38 Species: Caenorhabditis briggsae Genotype: Cbr-tra-1(nm2)/Cbr-let(nm28) III. Description: When singled, hermaphrodites should throw 2/3 hermaphrodites and 1/2 nm2 XX males. The lethal appears to balance the nm2 allele pretty well, but precise recombination mapping has not been performed. The XX males maintain their phenotypic resemblance to the unbalanced strain and are probably not fertile due to obvious gonadal deficiencies. This strain has been successfully grown at 15C and 20C. Both strains appear to have complete penetrance of the mutant phenotypes. Mutagen: Outcrossed: x Made by: D. Kelleher Received: 08/18/08 University of Maryland, College Park, MD ----------------------------------------------------------------------------- Strain: CP4 Species: Caenorhabditis briggsae Genotype: Cbr-dpy-?(nm4) II. Description: Dpy. Tightly linked to Cbr-tra-2 on LG II. Molecular identity unknown. Reference: Kelleher DF, et al. Genetics. 2008 Mar;178(3):1415-29. Mutagen: EMS Outcrossed: x>6 Made by: Haag & Layton Received: 03/26/13 University of Maryland, College Park, MD ----------------------------------------------------------------------------- Strain: CP87 Species: Caenorhabditis briggsae Genotype: Cbr-fem-3(nm63) IV. Description: XO are self-fertile hermaphrodites with low brood size and some somatic gonad defects. XX animals have normal brood size. Mutagen: EMS Outcrossed: x4 Made by: Robin Cook Hill Received: 06/15/06 University of Maryland, College Park, MD ----------------------------------------------------------------------------- Strain: CP89 Species: Caenorhabditis briggsae Genotype: Cbr-fem-2(nm27) III; Cbr-fem-3(nm63) IV. Description: XX are self-fertile hermaphrodites. Mutagen: EMS Outcrossed: x Made by: Robin Cook Hill Received: 06/15/06 University of Maryland, College Park, MD ----------------------------------------------------------------------------- Strain: CP99 Species: Caenorhabditis briggsae Genotype: Cbr-unc-119(nm67) III. Description: Derived from AF16. Outcrossed >6x to AF16. Unc, slightly Dpy, no dauer formation (similar to C. elegans unc-119). nm67 is a deletion (827 bp) in Cbr-119 begining in exon 1 and ending 3' of exon 4. Reference: Liu Q, et al. Development. 2012 Apr;139(8):1509-21. Mutagen: EMS Outcrossed: x>6 Made by: Cristel Thomas Received: 03/26/13 University of Maryland, College Park, MD ----------------------------------------------------------------------------- Strain: CS1 Species: Caenorhabditis elegans Genotype: sma-9(wk55) X. Description: Small body size. Crumpled spicules. Male ray 8-9 fusion. Mutagen: EMS Outcrossed: x1 Made by: C Savage-Dunn Received: 02/05/04 Queens College, CUNY ----------------------------------------------------------------------------- Strain: CS119 Species: Caenorhabditis elegans Genotype: sma-3(wk30) III; him-5(e1490) V; qcEx24. Description: qcEx24 [GFP::sma-3 + rol-6(su1006)]. Rollers. Pick rollers to maintain. Array rescues body size phenotype. Reference: Wang J, Tokarz R, Savage-Dunn C. Development. 2002 Nov;129(21):4989-98. Mutagen: Outcrossed: x Made by: Jianjun Wang Received: 02/21/13 Queens College, CUNY ----------------------------------------------------------------------------- Strain: CS210 Species: Caenorhabditis elegans Genotype: sma-3(wk30) III; him-5(e1490) V; qcEx52. Description: qcEx52 [myo-2p::GFP::sma-3 + rol-6(su1006)]. Sma. Rollers. Pick rollers to maintain. Reference: Wang J, Tokarz R, Savage-Dunn C. Development. 2002 Nov;129(21):4989-98. Mutagen: Outcrossed: x Made by: Jianjun Wang Received: 02/21/13 Queens College, CUNY ----------------------------------------------------------------------------- Strain: CS211 Species: Caenorhabditis elegans Genotype: sma-3(wk30) III; him-5(e1490) V; qcEx53. Description: qcEx53 [vha-6p::GFP::sma-3 + rol-6(su1006)]. Sma. Rollers. Pick rollers to maintain. Reference: Wang J, Tokarz R, Savage-Dunn C. Development. 2002 Nov;129(21):4989-98. Mutagen: Outcrossed: x Made by: Jianjun Wang Received: 02/21/13 Queens College, CUNY ----------------------------------------------------------------------------- Strain: CS389 Species: Caenorhabditis elegans Genotype: adt-2(wk156) X. Description: Sma. Reference: Fernando T, et al. Dev Biol. 2011 Apr 1;352(1):92-103. Mutagen: EMS Outcrossed: x4 Made by: Thilini Fernando Received: 02/21/13 Queens College, CUNY ----------------------------------------------------------------------------- Strain: CS619 Species: Caenorhabditis elegans Genotype: sma-3(wk30) III; qcIs59. Description: qcIs59 [vha-6p::GFP::sma-3 + rol-6(su1006)]. Small Rollers. GFP::SMA-3 fusion protein is expressed in the intestine. Derivative of a strain described in Wang et al., 2002, Development 129:4989-4998. Mutagen: Outcrossed: x1 Made by: Uday Madaan Received: 03/31/15 Queens College, CUNY ----------------------------------------------------------------------------- Strain: CS624 Species: Caenorhabditis elegans Genotype: daf-2(e1370) III; lon-2(e678) X. Description: Temperature-sensitive. Maintain at 15C. Lon. Daf-c. Mutagen: Outcrossed: x Made by: James Clark Received: 03/31/15 Queens College, CUNY ----------------------------------------------------------------------------- Strain: CS67 Species: Caenorhabditis elegans Genotype: sma-9(qc3) X. Description: Small body size. Crumpled spicules. Male ray 8-9 fusion. Mutagen: EMS Outcrossed: x1 Made by: C Savage-Dunn Received: 02/05/04 Queens College, CUNY ----------------------------------------------------------------------------- Strain: CSM1320 Species: C. elegans Genotype: twk-2(mac507) II. Description: twk-2 loss-of-function allele. Moderately deep curvature. Weakly extended backward locomotion. Frequent backward locomotion. Reference: Zhou C, et al. PLoS Genet. 2022;18: e1010126. doi:10.1371/journal.pgen.1010126. PMID: 35482723. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Chuanman Zhou Received: 11/19/24 Central South University, Changsha, China ----------------------------------------------------------------------------- Strain: CSM1323 Species: C. elegans Genotype: twk-7(mac510) III. Description: twk-7 loss-of-function allele. Hyperactive forward locomotion. twk-7(mac510) is a 10-bp deletion and 2-bp insertion in exon 9, causing a frameshift: aatttattttcagGTAAAAAAGAACGCAGCAACGGAGACATGGACATTTTCATCGTCCATTTTCTTTGCCGTAACCGTCGTCACTACCATCGGATACGGTAATCCAGTTCCAGTGACAAACATTGGACGGATATGGTGTATATTGTTCTCCTTGCTTGGAA(TACCTCTAAC)del(AA)insACTGGTTACCATCGCTGACTTGGgtaagtgg. Reference: Zhou C, et al. PLoS Genet. 2022;18: e1010126. doi:10.1371/journal.pgen.1010126. PMID: 35482723. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Chuanman Zhou Received: 11/19/24 Central South University, Changsha, China ----------------------------------------------------------------------------- Strain: CSM1331 Species: C. elegans Genotype: twk-43(mac518) V. Description: twk-43 loss-of-function allele. Reference: Zhou C, et al. PLoS Genet. 2022;18: e1010126. doi:10.1371/journal.pgen.1010126. PMID: 35482723. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Chuanman Zhou Received: 11/19/24 Central South University, Changsha, China ----------------------------------------------------------------------------- Strain: CSM1356 Species: C. elegans Genotype: twk-26(mac525) X. Description: twk-26 loss-of-function allele. Reference: Zhou C, et al. PLoS Genet. 2022;18: e1010126. doi:10.1371/journal.pgen.1010126. PMID: 35482723. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Chuanman Zhou Received: 11/19/24 Central South University, Changsha, China ----------------------------------------------------------------------------- Strain: CSM1358 Species: C. elegans Genotype: twk-29(mac527) I. Description: twk-29 loss-of-function allele. Reference: Zhou C, et al. PLoS Genet. 2022;18: e1010126. doi:10.1371/journal.pgen.1010126. PMID: 35482723. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Chuanman Zhou Received: 11/19/24 Central South University, Changsha, China ----------------------------------------------------------------------------- Strain: CSM1360 Species: C. elegans Genotype: twk-30(mac529) I. Description: twk-30 loss-of-function allele. Extended backward locomotion. Reference: Zhou C, et al. PLoS Genet. 2022;18: e1010126. doi:10.1371/journal.pgen.1010126. PMID: 35482723. Mutagen: Outcrossed: x0 Made by: Chuanman Zhou Received: 11/19/24 Central South University, Changsha, China ----------------------------------------------------------------------------- Strain: CSM1362 Species: C. elegans Genotype: twk-35(mac531) V. Description: twk-35 loss-of-function allele. Reference: Zhou C, et al. PLoS Genet. 2022;18: e1010126. doi:10.1371/journal.pgen.1010126. PMID: 35482723. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Chuanman Zhou Received: 11/19/24 Central South University, Changsha, China ----------------------------------------------------------------------------- Strain: CSM1364 Species: C. elegans Genotype: twk-48(mac533) III. Description: twk-48 loss-of-function allele. Irregular curvature. Frequent deep forward turns. Brief forward coiling upon completing backward locomotion. Reference: Zhou C, et al. PLoS Genet. 2022;18: e1010126. doi:10.1371/journal.pgen.1010126. PMID: 35482723. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Chuanman Zhou Received: 11/19/24 Central South University, Changsha, China ----------------------------------------------------------------------------- Strain: CSM1366 Species: C. elegans Genotype: twk-49(mac535) II. Description: twk-49 loss-of-function allele. Reference: Zhou C, et al. PLoS Genet. 2022;18: e1010126. doi:10.1371/journal.pgen.1010126. PMID: 35482723. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Chuanman Zhou Received: 11/19/24 Central South University, Changsha, China ----------------------------------------------------------------------------- Strain: CT11 Species: Caenorhabditis elegans Genotype: hbl-1(mg285) X. Description: Egl. Slightly Pvl. Mutagen: EMS Outcrossed: x10 Made by: Slack & Pasquinelli Received: 01/06/04 Beth Israel Deaconess Medical Center ----------------------------------------------------------------------------- Strain: CT12 Species: Caenorhabditis elegans Genotype: zaEx5. Description: zaEx5 [let-7::GFP + rol-6(su1006)]. Pick GFP+ to maintain. The GFP+ animals may not always express the Roller phenotype. Mutagen: Outcrossed: x Made by: Steven Johnson Received: 08/22/07 Beth Israel Deaconess Medical Center ----------------------------------------------------------------------------- Strain: CT15 Species: Caenorhabditis elegans Genotype: zaEx16. Description: zaEx16 [mir-84::GFP + rol-6(su1006)]. Pick GFP+ to maintain. The GFP+ animals may not always express the Roller phenotype. Mutagen: Outcrossed: x Made by: Steven Johnson Received: 08/22/07 Beth Israel Deaconess Medical Center ----------------------------------------------------------------------------- Strain: CT16 Species: Caenorhabditis elegans Genotype: zaEx17. Description: zaEx17 [lin-4::GFP + rol-6(su1006)]. Pick GFP+ to maintain. The GFP+ animals may not always express the Roller phenotype. Mutagen: Outcrossed: x Made by: Lei Bei Received: 08/22/07 Beth Israel Deaconess Medical Center ----------------------------------------------------------------------------- Strain: CT17 Species: Caenorhabditis elegans Genotype: zaEx18. Description: zaEx18 [mir-237::GFP + rol-6(su1006)]. Pick GFP+ to maintain. The GFP+ animals may not always express the Roller phenotype. Mutagen: Outcrossed: x Made by: A. Esquela-Kerscher Received: 08/22/07 Beth Israel Deaconess Medical Center ----------------------------------------------------------------------------- Strain: CT18 Species: Caenorhabditis elegans Genotype: zaEx19. Description: zaEx19 [mir-48::GFP + rol-6(su1006)]. Pick GFP+ to maintain. The GFP+ animals may not always express the Roller phenotype. Mutagen: Outcrossed: x Made by: J Patridge/K Saito Received: 08/22/07 Beth Israel Deaconess Medical Center ----------------------------------------------------------------------------- Strain: CT20 Species: Caenorhabditis elegans Genotype: zaIs5. Description: zaIs5 [alg-1p::GFP::alg-1 + rol-6(su1006)]. Some GFP+ animals do not roll. GFP::ALG-1 expression throughout development, detectable as punctate cytoplasmic granules. Maintain by picking rollers. Reference: Chan SP, Slack FJ. Dev Biol. 2009 Oct 1;334(1):152-60. Mutagen: UV Outcrossed: x3 Made by: Shih-Peng Chan Received: 02/01/12 Beth Israel Deaconess Medical Center ----------------------------------------------------------------------------- Strain: CT21 Species: Caenorhabditis elegans Genotype: zaIs2. Description: zaIs2 [lin-14::GFP + rol-6(su1006)]. Rollers, though Rol is not completely penetrant, Reference: Olsson-Carter K, Slack FJ. PLoS Genet. 2010 Aug 5;6(8). pii: e1001054. Mutagen: Outcrossed: x Made by: Michelle Boehm Received: 04/30/12 Beth Israel Deaconess Medical Center ----------------------------------------------------------------------------- Strain: CT22 Species: Caenorhabditis elegans Genotype: zaIs6. Description: zaIs6 [mir-239.1p::GFP + myo-2p::DsRed]. Reference: de Lencastre A, et al. Curr Biol. 2010 Dec 21;20(24):2159-68. Mutagen: UV/TMP Outcrossed: x7 Made by: Katherine Zhou Received: 06/15/12 Beth Israel Deaconess Medical Center ----------------------------------------------------------------------------- Strain: CT8 Species: Caenorhabditis elegans Genotype: lin-41(ma104) I. Description: Dpy. Precocious heterochronic. Reduced brood size. There may be a linked Dpy mutation in this strain. Mutagen: mutator TR679 Outcrossed: x Made by: V. Ambros Received: 12/07/01 Beth Israel Deaconess Medical Center ----------------------------------------------------------------------------- Strain: CU1546 Species: Caenorhabditis elegans Genotype: smIs34. Description: smIs34 [ced-1p::ced-1::GFP + rol-6(su1006)]. Maintain under standard conditions. Reference: Zou W, et al., (2009) PLoS Genet. 5(10):e1000679. Mutagen: gamma irradiation Outcrossed: x3 Made by: Xiaochen Wang Received: 09/17/10 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CU1715 Species: Caenorhabditis elegans Genotype: psr-1(tm469) IV. Description: 968 bp deletion. Engulfment defects. Mutagen: UV/TMP Outcrossed: x4 Made by: X. Wang & D. Xue Received: 04/01/04 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CU2904 Species: Caenorhabditis elegans Genotype: scrm-1(tm698) I. Description: Mild defect in cell corpse engulfment. Mutagen: UV/TMP Outcrossed: x4 Made by: X. Wang & D. Xue Received: 06/27/06 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CU2945 Species: Caenorhabditis elegans Genotype: scrm-1(tm805) I. Description: Mild defect in cell corpse engulfment. Mutagen: UV/TMP Outcrossed: x4 Made by: X. Wang & D. Xue Received: 06/27/07 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CU394 Species: Caenorhabditis elegans Genotype: smIs10 I. Description: smIs10 [ced-3p::ced-3::GFP + rol-6(su1006)] I. Rollers. Reference: Geng X, et al. Nat Struct Mol Biol. 2008 Oct;15(10):1094-101. Mutagen: Gamma ray Outcrossed: x8 Made by: Beth Kimberly Received: 08/12/13 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CU5991 Species: Caenorhabditis elegans Genotype: fzo-1(tm1133) II. Description: Slow growth. Reference: Breckenridge DG, et al. Mol Cell. 2008 Aug 22;31(4):586-97. Mutagen: TMP/UV Outcrossed: x4 Made by: David Breckenridge Received: 02/14/14 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CU6102 Species: Caenorhabditis elegans Genotype: skr-1(sm151) I; unc-76(e911) V. Description: sm151 is a semi-dominant allele of skr-1. Maintain under normal conditions. Reference: Killian DJ, et al. (2008) Dev Biol. 322(2):322-31. Mutagen: UV/TMP Outcrossed: x Made by: Darrell Killian Received: 09/17/10 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CU6372 Species: Caenorhabditis elegans Genotype: drp-1(tm1108) IV. Description: Homozygous viable. Slow growth but not lethal or sterile. Reference: Breckenridge DG, et al. Mol Cell. 2008 Aug 22;31(4):586-97. Mutagen: TMP/UV Outcrossed: x9 Made by: David Breckenridge Received: 02/04/14 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CU6493 Species: Caenorhabditis elegans Genotype: eef-1A.1(q145) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT and GFP+. Segregate non-GFP steriles (q145 homozygotes). qIs48 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter driving GFP in the intestine, and is homozygous lethal. Mutagen: Outcrossed: x Made by: Huey-Jen Lai Received: 10/09/07 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CU7905 Species: Caenorhabditis elegans Genotype: smIs350 IV; unc-76(e911) V. Description: smIs350 [hsp-16::mCherry-NLS + tra-2::FLAG(3x) + unc-76(+)] IV. Some sterility. Maintain under normal conditions. Reference: Mapes J, et al. (2010) PNAS In press. Mutagen: gamma irradiation Outcrossed: x0 Made by: Jim Mapes Received: 09/17/10 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CU9087 Species: Caenorhabditis elegans Genotype: unc-76(e911) V; smIs380. Description: smIs380 [tra-2::GFP + unc-76(+)]. Some sterility. Maintain under normal conditions. Reference: Mapes J, et al. (2010) PNAS In press. Mutagen: gamma irradiation Outcrossed: x0 Made by: Jim Mapes Received: 09/17/10 University of Colorado, Boulder, CO ----------------------------------------------------------------------------- Strain: CV138 Species: Caenorhabditis elegans Genotype: sgo-1(tm2443) IV. Description: tm2443 is a 204 bp deletion + 7 bp insertion in 21762/21763-TTTTCTC-21966/21967. A low penetrance (1/25) of chromosome bridges is observed at anaphase I. Reference: de Carvalho et al., Genes Dev 22, 2869-2885. Mutagen: UV/TMP Outcrossed: x5 Made by: Monica Colaiacovo Received: 10/27/09 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CV199 Species: Caenorhabditis elegans Genotype: fan-1(tm423) IV/nT1 [qIs51] (IV;V). Description: Maintain under normal condition. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP fan-1 homozygotes. Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Smogorzewska A, et al. (2010) Molec Cell 39:36-47. Mutagen: UV/TMP Outcrossed: x6 Made by: Takamune Saito Received: 09/08/10 Harvard Medical School, Boston, MA ----------------------------------------------------------------------------- Strain: CV2 Species: Caenorhabditis elegans Genotype: syp-3(ok758)/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT and GFP+. Segregate syp-3(ok758) homozygotes that are non-GFP and lay mostly dead eggs; these mutants lack synaptonemal complex formation during meiosis. Homozygous hT2[bli-4 let-? qIs48] animals are inviable. Mutagen: UV/TMP Outcrossed: x4 Made by: M Colaiacovo Received: 09/28/07 Harvard Medical School, Boston, MA ----------------------------------------------------------------------------- Strain: CV203 Species: Caenorhabditis elegans Genotype: rjSi1 II. Description: rjSi1 [cra-1p::cra-1::GFP::cra-1 3'UTR + Cbr-unc-119(+)] II. Single copy insertion. cra-1 promoter, cra-1::GFP and 3'UTR was cloned into pCFJ150 (ttTi5605) vector and inserted into ttTi5605 of EG4322 strain. Outcrossed three times to N2 Bristol; could still carry unc-119(ed9) in the background. Superficially wild-type. This CRA-1::GFP fusion construct has been shown to be functional and its localization reflects endogenous CRA-1 localization. rjSi1 transgene can rescue synapsis defects of cra-1 mutants and restore cross-over events (six bivalents instead of the 11 to 12 univalents characteristic of cra-1 mutants). Brood size and embryonic lethality were significantly, albeit not completely, restored in the rescued line suggesting that the GFP tag might affect other CRA-1 functions. Reference: Gao J, et al. PLOS Genetics 11(3): e1005029. https://doi.org/10.1371/journal.pgen.1005029 Mutagen: MosSCI Outcrossed: x3 Made by: Hyun-Min Kim Received: 09/09/21 Harvard Medical School, Boston, MA ----------------------------------------------------------------------------- Strain: CV385 Species: Caenorhabditis elegans Genotype: acer-1(rj15) II. Description: acer-1(rj15) is a 7 nt deletion (removes nt 35-41 from the start codon) resulting in an out-of-frame deletion. Increased histone acetylation. Reference: Gao J, et al., PLoS Genet. 2015 Mar 13;11(3):e1005029. Mutagen: Outcrossed: x6 Made by: Jinmin Gao Received: 07/20/15 Harvard Medical School, Boston, MA ----------------------------------------------------------------------------- Strain: CV6 Species: Caenorhabditis elegans Genotype: lab-1(tm1791) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are wild-type GFP+. Homozygotes (tm1791/tm1791) are 4% Him with 22% embryonic lethality. Maintain by picking GFP+. Reference: de Carvalho et al., Genes Dev 22, 2869-2885. Mutagen: UV/TMP Outcrossed: x6 Made by: Monica Colaiacovo Received: 10/27/09 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CV78 Species: Caenorhabditis elegans Genotype: cra-1(tm2144) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Homozygous lethal allele balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP cra-1(tm2144) homozygotes (99.7% embryonic lethality, 61% larval lethality, Him). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Smolikove S, et al. (2008) PLoS Genet 4(6):e1000088. Mutagen: UV/TMP Outcrossed: x6 Made by: Sarit Smolikove Received: 09/08/10 Harvard Medical School, Boston, MA ----------------------------------------------------------------------------- Strain: CV87 Species: Caenorhabditis elegans Genotype: syp-4(tm2713) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Homozygous lethal allele balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP syp-4(tm2713) homozygotes (viable but throw 97% dead eggs, 40% males). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Smolikove S, et al. (2009) PLoS Genet 5(10):e1000669. Mutagen: UV/TMP Outcrossed: x6 Made by: Sarit Smolikove Received: 09/08/10 Harvard Medical School, Boston, MA ----------------------------------------------------------------------------- Strain: CV98 Species: Caenorhabditis elegans Genotype: him-18(tm2181)/qC1 [dpy-19(e1259) glp-1(q339) qIs26] III. Description: qIs26 [lag-2::GFP + rol-6(su1006)]. Heterozygous animals show roller phenotype and GFP signal at the distal tip cells. Segregates roller GFP(+) heterozygotes, wild-type moving GFP(-) him-18(tm2181) homozygotes. qC1 [dpy-19(e1259) glp-1(q339) qIs26] homozygous animals are dead. P0 him-18(tm2181) homozygous animals show 80% embryonic lethality and 12% high incidence of male at F1. Pick roller GFP(+) worms to maintain. Reference: Saito TT, et al. (2009) PLoS Genet 5:e1000735. Mutagen: UV/TMP Outcrossed: x6 Made by: Takamune Saito Received: 09/08/10 Harvard Medical School, Boston, MA ----------------------------------------------------------------------------- Strain: CVB96 Species: C. elegans Genotype: siss-1(csn20) IV. Description: siss-1(csn20) animals are defective in stress-induced sleep (SIS) with no other obvious defects in development or behavior. csn20 is a Cys-to-Tyr substitution in the third Cys residue of the EGF motif of SISS-1 (a.k.a. IGEG-1). csn20 phenocopies the deletion allele ve532, indicating that the EGF domain of SISS-1 is functional. Reference: Hill AJ, et al. Nat Commun 15, 10886. doi: 10.1038/s41467-024-55252-4. PMID: 39738055. Mutagen: EMS Outcrossed: x3 Made by: Bryan Robinson Received: 01/16/25 California State University Northridge, Northridge, CA ----------------------------------------------------------------------------- Strain: CW129 Species: Caenorhabditis elegans Genotype: unc-9(fc16) X. Description: Mutagen: EMS Outcrossed: x Made by: Marge Sedensky Received: 03/12/02 Seattle Children's Research Institute ----------------------------------------------------------------------------- Strain: CW152 Species: Caenorhabditis elegans Genotype: gas-1(fc21) X. Description: Hypersensitive to volatile anesthetics. Temperature sensitive hypomorph and should be propagated at or below 20C. Low brood size. Mutagen: EMS Outcrossed: x3 Made by: Phil Morgan Received: 06/18/99 Seattle Children's Research Institute ----------------------------------------------------------------------------- Strain: CW16 Species: Caenorhabditis elegans Genotype: unc-79(ec1) III. Description: Mutagen: EMS Outcrossed: x Made by: Phil Morgan Received: 03/12/02 Seattle Children's Research Institute ----------------------------------------------------------------------------- Strain: CX1 Species: Caenorhabditis elegans Genotype: odr-6(ky1) II. Description: Mutagen: Outcrossed: x Made by: Cori Bargmann Received: 12/27/95 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX10 Species: Caenorhabditis elegans Genotype: osm-9(ky10) IV. Description: diac-. Mutagen: Outcrossed: x Made by: Heather Colber Received: 08/07/97 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX10207 Species: Caenorhabditis elegans Genotype: calf-1(ky867) V. Description: Saheki Y, Bargmann CI. Nat Neurosci. 2009 Oct;12(10):1257-65. Mutagen: Outcrossed: x Made by: Yasunori Saheki Received: 05/13/13 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX11262 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Reference: Andersen EC, et al. Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: A. Sivasundar Received: 09/04/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: CX11264 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Reference: Andersen EC, et al. Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: A. Sivasundar Received: 09/04/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: CX11271 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: A. Sivasundar Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: CX11276 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: A. Sivasundar Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: CX11285 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: A. Sivasundar Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: CX11292 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: A. Sivasundar Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: CX11307 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: A. Sivasundar Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: CX11314 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: A. Sivasundar Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: CX11315 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: A. Sivasundar Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: CX11400 Species: Caenorhabditis elegans Genotype: kyIR9 (X: ~4745910 - ~4927296, N2>CB4856) X. Description: kyIR9 [X: ~4745910 - ~4927296, N2>CB4856] X. LG X contains N2 from ~4745910 - ~4927296; the rest is from CB4856. QX9 was crossed to CB4856; a recombinant F2 with N2 npr-1 and CB4856 tyra-3 was isolated. Its progeny was back-crossed to CB4856 8 times, picking npr-1 hets each round prior to homozygosing. Mutagen: Outcrossed: x8 Made by: Andres Bendesky Received: 05/11/12 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX11839 Species: Caenorhabditis elegans Genotype: tyra-3(ok325) X. Description: Reference: Flavell SW, et al. Cell. 2013 Aug 29;154(5):1023-35. Mutagen: Outcrossed: x4 Made by: Steven Flavell Received: 10/11/13 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX12708 Species: Caenorhabditis elegans Genotype: ggr-2(lf62) X. Description: Reference: Flavell SW, et al. Cell. 2013 Aug 29;154(5):1023-35. Mutagen: Outcrossed: x4 Made by: Steven Flavell Received: 03/15/25 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX12709 Species: Caenorhabditis elegans Genotype: avr-14(ad1302) I. Description: Decreased locomotion on food. Reference: Flavell SW, et al. Cell. 2013 Aug 29;154(5):1023-35. Mutagen: Outcrossed: x4 Made by: Steven Flavell Received: 10/11/13 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX12724 Species: Caenorhabditis elegans Genotype: acc-4(ok2371) III. Description: Reference: Flavell SW, et al. Cell. 2013 Aug 29;154(5):1023-35. Mutagen: Outcrossed: x4 Made by: Steven Flavell Received: 10/11/13 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX12725 Species: Caenorhabditis elegans Genotype: inx-20(ok681) I. Description: Increased locomotion on food. Reference: Flavell SW, et al. Cell. 2013 Aug 29;154(5):1023-35. Mutagen: Outcrossed: x4 Made by: Steven Flavell Received: 10/16/13 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX12726 Species: Caenorhabditis elegans Genotype: inx-9(ok1502) IV. Description: Increased locomotion on food. Reference: Flavell SW, et al. Cell. 2013 Aug 29;154(5):1023-35. Mutagen: Outcrossed: x4 Made by: Steven Flavell Received: 10/11/13 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX12800 Species: Caenorhabditis elegans Genotype: ser-3(ad1774) I. Description: Reference: Flavell SW, et al. Cell. 2013 Aug 29;154(5):1023-35. Mutagen: Outcrossed: x5 Made by: Steven Flavell Received: 10/16/13 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX13079 Species: Caenorhabditis elegans Genotype: octr-1(ok371) X. Description: Derived by outcrossing VC224 four times to N2. Reference: Flavell SW, et al. Cell. 2013 Aug 29;154(5):1023-35. Mutagen: Outcrossed: x4 Made by: Steven Flavell Received: 10/16/13 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX13111 Species: Caenorhabditis elegans Genotype: dop-5(ok568) V. Description: Derived by outcrossing RB785 three times to N2. Reference: Flavell SW, et al. Cell. 2013 Aug 29;154(5):1023-35. Mutagen: Outcrossed: x3 Made by: Steven Flavell Received: 10/11/13 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX13325 Species: Caenorhabditis elegans Genotype: inx-2(ok376) X. Description: Derived by outcrossing VC260 four times to N2. Reference: Flavell SW, et al. Cell. 2013 Aug 29;154(5):1023-35. Mutagen: Outcrossed: x4 Made by: Steven Flavell Received: 10/11/13 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX14295 Species: Caenorhabditis elegans Genotype: pdfr-1(ok3425) III. Description: Decreased locomotion on food. Derived by outcrossing VC2609 five times to N2. Reference: Flavell SW, et al. Cell. 2013 Aug 29;154(5):1023-35. Mutagen: Outcrossed: x5 Made by: Steven Flavell Received: 10/11/13 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX14394 Species: Caenorhabditis elegans Genotype: npr-5(ok1583) V. Description: Derived by outcrossing RB1393 five times to N2. Reference: Flavell SW, et al. Cell. 2013 Aug 29;154(5):1023-35. Mutagen: Outcrossed: x5 Made by: Steven Flavell Received: 03/15/25 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX17256 Species: Caenorhabditis elegans Genotype: kyIs722. Description: kyIs722 [str-2p::GCaMP5(D380Y) + elt-2::mCherry]. GCaMP5a expression driven by str-2 promoter, providing a very bright integrated calcium indicator useful for imaging of AWC(on). Mutagen: UV/TMP Outcrossed: x6 Made by: Sagi Levy Received: 10/02/19 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX188 Species: Caenorhabditis elegans Genotype: dig-1(ky188) III; kyIs4 X. Description: kyIs4 [ceh-23p::unc-76::GFP + lin-15(+)] X. kyIs4 contains a fragment of unc-76 protein that drives enrichment of GFP in the axons. Posteriorly displaced nerve ring axons. This strain may contain lin-15(n765) X. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x3 Made by: Jen Zallen Received: 07/24/00 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX20 Species: Caenorhabditis elegans Genotype: adp-1(ky20) II. Description: Defective in adaptation to a subset of AWC-sensed odorants. Dominant. Mutagen: EMS Outcrossed: x2 Made by: Colbert/Bargmann Received: 02/01/96 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX2065 Species: Caenorhabditis elegans Genotype: odr-1(n1936) X. Description: Defective chemotaxis to some volatile odorants: benzaldehyde, 2-butanone, isoamyl alcohol. [NOTE: the n1936 mutation is a G-to-A substitution at the end of the second exon (donor site). N2: 5'AGTTGAGGTAATTCA3'. n1936: 5'AGTTGAGATAATTCA3'. Recommended sequencing primers: FWD 5'gcaggagctcacatcggtta3'; REV 5'ttggaatcacatcctgcatga3'.] Mutagen: EMS Outcrossed: x2 Made by: C. Bargmann Received: 12/20/93 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX2205 Species: Caenorhabditis elegans Genotype: odr-3(n2150) V. Description: Defective chemotaxis to many volative odorants. Defective osmotic avoidance (osm). Defective chemotaxis to some water-soluble attractants (che). Mutagen: EMS Outcrossed: x3 Made by: C. Bargmann Received: 12/20/93 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX2304 Species: Caenorhabditis elegans Genotype: odr-2(n2145) V. Description: Defective chemotaxis to some volatile odorants: benzaldehyde, isoamyl alcohol. Mutagen: EMS Outcrossed: x3 Made by: C. Bargmann Received: 12/20/93 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX2357 Species: Caenorhabditis elegans Genotype: odr-5(ky9) X. Description: Defective chemotaxis to some volatile odorants: benzaldehyde, 2-butanone, isoamyl alcohol. Linked sterility has not been separated from Odr phenotype. Mutagen: EMS Outcrossed: x3 Made by: C. Bargmann Received: 12/20/93 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX2386 Species: Caenorhabditis elegans Genotype: odr-8(ky31) IV. Description: Mutagen: Outcrossed: x Made by: Piali Sengupta Received: 02/19/08 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX2398 Species: Caenorhabditis elegans Genotype: odr-8(ky41) IV. Description: Mutagen: Outcrossed: x Made by: Piali Sengupta Received: 01/28/08 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX2565 Species: Caenorhabditis elegans Genotype: kyIs4 lin-15B&lin-15A(n765) X. Description: kyIs4 [ceh-23p::unc-76::GFP + lin-15(+)] X. kyIs4 contains a fragment of unc-76 protein that drives enrichment of GFP in the axons. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Jen Zallen Received: 09/12/03 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX2610 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) kyIs30 X. Description: kyIs30 [glr-1::GFP + lin-15(+)]. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Villu Maricq Received: 09/12/03 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX2914 Species: Caenorhabditis elegans Genotype: nDf16/dpy-17(e164) unc-32(e189) III. Description: Heterozygotes are WT and segregate WT, DpyUncs and dead eggs. The DpyUncs will outgrow the hets. Mutagen: Outcrossed: x Made by: Noelle Dwyer Received: 10/28/94 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX2993 Species: Caenorhabditis elegans Genotype: sax-7(ky146) IV; kyIs4 X. Description: kyIs4 [ceh-23p::unc-76::GFP + lin-15(+)] X. kyIs4 contains a fragment of unc-76 protein that drives enrichment of GFP in the axons. Posteriorly displaced nerve ring axons. This strain may contain lin-15(n765) X. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x3 Made by: Jen Zallen Received: 07/24/00 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX3019 Species: Caenorhabditis elegans Genotype: mut-2(r459) I; dpy-19(n1347) glr-1(ky176) III. Description: Nose touch defective (recessive). Mechanosensory defective (semi-dominant). Mildly Dpy (ts). Mutagen: Tc1 Outcrossed: x10 Made by: A. Maricq Received: 12/27/95 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX3125 Species: Caenorhabditis elegans Genotype: sax-6(ky214) I; kyIs4 X. Description: kyIs4 [ceh-23p::unc-76::GFP + lin-15(+)] X. kyIs4 contains a fragment of unc-76 protein that drives enrichment of GFP in the axons. sax-6 is temperature sensitive. Posterior axon from amphid neuron. This strain may contain lin-15(n765) X. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Jen Zallen Received: 07/24/00 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX3137 Species: Caenorhabditis elegans Genotype: sax-9(ky212) IV; kyIs4 X. Description: kyIs4 [ceh-23p::unc-76::GFP + lin-15(+)] X. kyIs4 contains a fragment of unc-76 protein that drives enrichment of GFP in the axons. sax-9 is temperature senstive. Amphid axon guidance defects (termination, guidance). This strain may contain lin-15(n765) X. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Jen Zallen Received: 07/24/00 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX3171 Species: Caenorhabditis elegans Genotype: sax-3(ky200) X. Description: Defective in the axon guidance of neurons. Maintain at 20C or 25C. Mutagen: EMS Outcrossed: x Made by: Jen Zallen Received: 09/09/04 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX3198 Species: Caenorhabditis elegans Genotype: sax-3(ky123) X. Description: sax-3 mutants have an anteriorly-displaced nerve ring, defects in axon guidance to the ventral midline, and extra axon crossing at the ventral midline. There are also defects in CAN and HSN cell migration, a notched head and an Egl phenotype. This allele is 80% lethal in embryonic stages. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x3 Made by: Jen Zallen Received: 03/23/98 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX32 Species: Caenorhabditis elegans Genotype: odr-10(ky32) X. Description: Impaired chemotaxis to low concentrations of the odorant diacetyl. Mutagen: EMS Outcrossed: x4 Made by: Piali Sengupta Received: 05/13/96 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX3222 Species: Caenorhabditis elegans Genotype: odr-3(n1605) V. Description: Reference: Roayaie K, et al. Neuron. 1998 Jan;20(1):55-67. Mutagen: Outcrossed: x Made by: Jennifer Whangbo Received: 12/11/12 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX3260 Species: Caenorhabditis elegans Genotype: kyIs37 II. Description: kyIs37 [odr-10::GFP + lin-15(+)] II. No lin-15 mutation in background. Translational fusion; first few amino acids of odr-10. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x3 Made by: Piali Sengupta Received: 09/12/03 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX3344 Species: Caenorhabditis elegans Genotype: kyIs53 X. Description: kyIs53[odr-10::GFP]. Full length odr-10. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x3 Made by: Noelle Dwyer Received: 09/12/03 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX3385 Species: Caenorhabditis elegans Genotype: sax-2(ky216) III. Description: Temperature sensitive. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x4 Made by: Jen Zallen Received: 07/24/00 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX3410 Species: Caenorhabditis elegans Genotype: odr-10(ky225) X. Description: Impaired chemotaxis to low concentrations of the odorant diacetyl. ky225 is a 1351 bp deletion removing all coding sequence past the N-terminal 120 amino acids. Mutagen: Tc1 Outcrossed: x4 Made by: Joseph Chou Received: 05/13/96 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX3465 Species: Caenorhabditis elegans Genotype: kyIs39. Description: kyIs39 [sra-6::GFP + lin-15(+)]. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Scott Clark Received: 12/15/03 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX3493 Species: Caenorhabditis elegans Genotype: sax-5(ky118) IV. Description: Amphid axon guidence defect. HSN axon guidence defect. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x3 Made by: Jen Zallen Received: 07/24/00 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX3553 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) kyIs104 X. Description: kyIs104 [str-1p::GFP] X. AWB expression of GFP. Mutagen: UV/TMP Outcrossed: x2 Made by: E. Troemel Received: 12/07/04 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX3572 Species: Caenorhabditis elegans Genotype: kyIs105 V; lin-15B&lin-15A(n765) X. Description: kyIs105 [str-3p::snb-1::GFP + lin-15(+)]. Also known as str-3::VAMP::GFP or ASI::VAMP::GFP or M7::VAMP::GFP. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x4 Made by: Gage Crump Received: 09/12/03 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX3596 Species: Caenorhabditis elegans Genotype: kyIs128 lin-15B&lin-15A(n765) X. Description: kyIs128 [str-3::GFP + lin-15(+)]. kyIs128 encodes a translational fusion contaning 4aa coding sequence (M7.13::GFP). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x2 Made by: Erin Peckol Received: 09/12/03 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX3662 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; kyIs121. Description: kyIs121 [unc-115::GFP + lin-15(+)]; autosomal. Maintain under normal conditions. Reference: Lundquist E, et al. (1998) Neuron 21(2):385-92. Mutagen: Outcrossed: x Made by: Erik Lundquist Received: 07/22/10 University of Kansas, Lawrence, KS ----------------------------------------------------------------------------- Strain: CX3695 Species: Caenorhabditis elegans Genotype: kyIs140 I. Description: kyIs140 [str-2::GFP + lin-15(+)] I. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x4 Made by: E. Troemel Received: 10/24/03 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX3709 Species: Caenorhabditis elegans Genotype: unc-115(ky275) X. Description: Unc. Lon. Mutagen: EMS Outcrossed: x5 Made by: Erik Lundquist Received: 08/17/07 University of Kansas, Lawrence, KS ----------------------------------------------------------------------------- Strain: CX3716 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) kyIs141 X. Description: kyIs141[osm-9::GFP5 + lin-15(+)]. GFP in sensory neurons. Mutagen: UV/TMP Outcrossed: x0 Made by: Tracy Smith Received: 12/21/04 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX3877 Species: Caenorhabditis elegans Genotype: kyIs156 X. Description: kyIs156 [str-1p::odr-10(cDNA)::GFP]. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Levente Egry Received: 09/12/03 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX3933 Species: Caenorhabditis elegans Genotype: kyIs140 I; slo-1(ky389) V. Description: kyIs140 [str-2::GFP + lin-15(+)]. ky389 is semi-dominant. str-2::GFP is expressed in both AWC neurons in ky389 mutants. PKA nsy-3(ky389). Mutagen: EMS Outcrossed: x3 Made by: Alvaro Sagasti Received: 01/26/00 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX3937 Species: Caenorhabditis elegans Genotype: lim-4(ky403) X. Description: Coily movement; aberrent head movement. The AWB neurons are transformed towards the AWC neuron fate by many criteria. Mutagen: EMS Outcrossed: x4 Made by: Alvaro Sagasti Received: 09/07/99 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX3940 Species: Caenorhabditis elegans Genotype: kyIs140 I; rol-6(e187) II; slo-1(ky399) V. Description: kyIs140 [str-2::GFP + lin-15(+)] I. In ky399 mutants str-2::GFP is expressed in both AWX neurons. ky399 is a semi-dominant allele of slo-1, a large conductance potassium channel. PKA nsy-3(ky399). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x1 Made by: Alvaro Sagasti Received: 02/06/04 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX4 Species: Caenorhabditis elegans Genotype: odr-7(ky4) X. Description: odr-7 mutants fail to respond to odorants sensed by the AWA neurons. Mutagen: EMS Outcrossed: x2 Made by: Received: 12/27/95 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX4103 Species: Caenorhabditis elegans Genotype: kyIs150 IV; sax-1(ky491) X. Description: kyIs150 [tax-2(delta)::GFP + lin-15(+)]. sax-1 is temperature-sensitive. ky491 was isolated by PCR from a deletion library. [NOTE: (12/29/2020) This strain has been found to actually be carrying the ky491 deletion allele of sax-1, not the ky211 point mutation as previously reported.] ky491 is a 1263 bp deletion in sax-1 (left flanking sequence: atgaagcccagg ctgtgaataaattgaatg, right flanking sequence: ccaatcacagtcagcctccgataaaatgtc). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Jen Zallen Received: 07/24/00 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX4148 Species: Caenorhabditis elegans Genotype: npr-1(ky13) X. Description: Mutagen: Outcrossed: x Made by: Received: 06/17/04 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX4533 Species: Caenorhabditis elegans Genotype: ocr-1(ok132) V. Description: Double mutants with ocr-2 have reduced AWA gene expression. Mutagen: Outcrossed: x6 Made by: David Tobin Received: 03/17/03 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX4534 Species: Caenorhabditis elegans Genotype: ocr-1(ak46) V. Description: Double mutants with ocr-2 have reduced AWA gene expression. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Tc1 Outcrossed: x5 Made by: D Tobin/Villu Maricq Received: 03/17/03 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX4544 Species: Caenorhabditis elegans Genotype: ocr-2(ak47) IV. Description: Chemosensory, mechanosensory, and osmosensory defects. Null allele. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Tc1 Outcrossed: x6 Made by: David Madsen Received: 03/04/03 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX4828 Species: Caenorhabditis elegans Genotype: kyIs140 I; tir-1(ky388) III; him-5(e1490) V. Description: kyIs140 [str-2::GFP + lin-15(+)] I. Him. In tir-1 mutants str-2::GFP is expressed in both AWC neurons. Mutagen: EMS Outcrossed: x4 Made by: Alvaro Sagasti Received: 01/26/00 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX4998 Species: Caenorhabditis elegans Genotype: kyIs140 I; nsy-1(ky397) II. Description: kyIs140 [str-2::GFP + lin-15(+)] I. In nsy-1 mutants str-2::GFP is expressed in both AWC neurons. Mutagen: EMS Outcrossed: x2 Made by: Alvaro Sagasti Received: 01/26/00 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX5000 Species: Caenorhabditis elegans Genotype: slt-1(eh15) X. Description: slt-1 mutants have no dissecting-scope phenotype. They have a 40% penetrant defect in the ventral guidance of the AVM neuron scored with mec-4::GFP, a mild defect in CAN cell migration that is enhanced by a ceh-23::GFP transgene, and a mild defect in midline crossing by PVQ neurons scorable with sra-6::GFP. slt-1(eh15) is a complex rearrangement that duplicates the endogenous slt-1 gene, but disrupts both duplicated copies. The two copies are linked on X but the exact distance between them is not known. The duplication probably extends >13 kb based on Southern blotting. Deletion breakpoints for the first copy of slt-1 are as follows: nucleotides 26219 to 28163 and 28197 to 28294 in cosmid C26G2 are deleted. The second copy of slt-1 contains the following structure: nucleotides 28197 to 28294 in C26G2 are deleted, followed by a duplication of nucleotides 28300 to 28396 in C26G2 that begins 5 nucleotides after the deletion. Both copies of slt-1 are mutant, as confirmed by both DNA sequence and RT-PCR analysis of slt-1 mRNA. Scoring for homozygosity of the slt-1 allele by PCR is difficult because of the two copies of the gene and because the small deletion and the small duplication of the second copy of slt-1 are the same size. The mutant can be followed indirectly by X linkage (very closely linked to unc-3). It may be possible to make a specific primer within the duplicated region that detects a unique band in the slt-1 mutant. Mutagen: Outcrossed: x2 Made by: David Hughes/Joe Hao Received: 11/26/01 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX51 Species: Caenorhabditis elegans Genotype: dyn-1(ky51) X. Description: ky51 animals are WT (or nearly WT) for locomotion, defecation rate, egg laying and fertility at 15C and 20C. Become Unc within 60 seconds when shifted to 25C. Recover when shifted back to 15C or 20C. dyn-1 encodes a dynamin GTPase. Received new stock from Cori Bargmann Jan 2005. Mutagen: EMS Outcrossed: x2 Made by: Scott Clark Received: 01/20/98 MRC - LMB, Cambridge, UK ----------------------------------------------------------------------------- Strain: CX5156 Species: Caenorhabditis elegans Genotype: sad-1(ky289) X. Description: Mutagen: Outcrossed: x Made by: Received: 03/01/01 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX5346 Species: Caenorhabditis elegans Genotype: slt-1(ev740) X. Description: AVM axon guidance defect. Mutagen: EMS Outcrossed: x Made by: J. Culotti Received: 12/07/04 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX5347 Species: Caenorhabditis elegans Genotype: slt-1(ev741) X. Description: AVM axon guidance defect. Mutagen: EMS Outcrossed: x Made by: J. Culotti Received: 12/07/04 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX5463 Species: Caenorhabditis elegans Genotype: slt-1(ok255) X. Description: Viable. Can be scored only using special neuronal markers such as zdIs5 [mec-4p::GFP + lin-15(+)], which labels the touch cells and shows that they have aberrant anterior processes in the slt-1 mutant. Mutagen: Outcrossed: x Made by: OMRF Knockout Group Received: 11/02/04 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX5478 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; kyEx581. Description: kyEx581 [ocr-4::GFP + lin-15(+)]. GFP expression in OLQS. Maintain by picking non-Muv. Mutagen: UV/TMP Outcrossed: x Made by: David Tobin Received: 12/21/04 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX5757 Species: Caenorhabditis elegans Genotype: kyIs140 I; nsy-4(ky616) IV. Description: kyIs140 [str-2::GFP + lin-15(+)] I. 2-AWC OFF. Mutagen: EMS Outcrossed: x4 Made by: Miri VanHoven Received: 04/10/07 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX5893 Species: Caenorhabditis elegans Genotype: kyIs140 I; ceh-36(ky646) X. Description: kyIs140 [str-2::GFP + lin-15(+)] I. In ceh-36 mutants, both AWC cells fail to take on the AWC fate. ceh-36 is also required for the specification of the ASEL identity. ceh-36 encodes a member of the OTX/OTD family of homeodomain proteins. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x4 Made by: Miri VanHoven Received: 04/23/04 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX5922 Species: Caenorhabditis elegans Genotype: kyIs140 I; ceh-36(ky640) X. Description: kyIs140 [str-2::GFP + lin-15(+)] I. In ceh-26 mutants, both AWC cells fail to take on the AWC fate. ceh-36 is also required for the specification of the ASEL identity. ceh-36 encodes a member of the OTX/OTD family of homeodomain proteins. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x4 Made by: Miri VanHoven Received: 04/23/04 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX5974 Species: Caenorhabditis elegans Genotype: kyIs262 IV. Description: kyIs262 [unc-86::myr::GFP + odr-1::RFP] IV. Mutagen: Outcrossed: x4 Made by: Carrie Adler Received: 04/30/12 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX6161 Species: Caenorhabditis elegans Genotype: inx-19(ky634) I. Description: Previously called nsy-5. Mutagen: EMS Outcrossed: x4 Made by: Miri VanHoven Received: 01/21/09 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX6391 Species: Caenorhabditis elegans Genotype: syg-2(ky671) X. Description: Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Originally had kyEx648 [unc-86 + syg-1::GFP] in this strain, but the CGC stock does not contain the array. Mutagen: EMS Outcrossed: x0 Made by: Kang Shen Received: 08/09/04 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX6448 Species: Caenorhabditis elegans Genotype: gcy-35(ok769) I. Description: 668 bp deletion in cosmid T04D3. Break points are 31961 and 32629 with respect to T04D3. Sequence at break point: CCTGCTCAATGACCTTTATCTTCGTT/AACGTGGCGAACAAAATGGAATCCAACGGT. Primers for a ~2.4kb band in ok769 and a ~3.1kb band in N2: ok769L 5' CCT GGT ACA GTA TTT AGG CG; 3' ok769R 5' CTT TCA GTC CGT TGA GCT TC 3'. Mutagen: UV/TMP Outcrossed: x6 Made by: Jesse Gray Received: 08/19/03 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX652 Species: Caenorhabditis elegans Genotype: kyIs235 V; syg-1(ky652) X. Description: kyIs235 [unc-86::snb-1::YFP + unc-4p::lin-10::RFP(intron) + odr-1::RFP]. Also known as unc-86::VAMP::YFP. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 01/20/04 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX6632 Species: Caenorhabditis elegans Genotype: kyEx728. Description: kyEx728 [sra-6p::G-CaMP]. No co-injection marker -- pick GFP+ animals to maintain. Mutagen: Outcrossed: x Made by: Amanda Kahn-Kirby Received: 12/28/12 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX6827 Species: Caenorhabditis elegans Genotype: eat-4(ky5) III; kyEx844. Description: kyEx844 contains [odr-3::eat-4 + elt-2::GFP]. Mutagen: Outcrossed: x Made by: Sreekanth Chalasani Received: 09/04/09 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX7102 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) qaIs2241 X. Description: qaIs2241 [gcy-36::egl-1 + gcy-35::GFP + lin-15(+)]. Mutagen: Outcrossed: x Made by: D Karow/J Gray Received: 06/20/08 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CX9683 Species: Caenorhabditis elegans Genotype: gcy-31(ok296) X; kyEx2116. Description: kyEx2116 contains [gcy-31::SL2::GFP + odr-1::DsRed2]. Mutagen: Outcrossed: x Made by: Manuel Zimmer Received: 08/25/09 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CY121 Species: Caenorhabditis elegans Genotype: ucp-4(ok195) V. Description: Amplify with the following external primers: EL1(agtcctgaacggagctttga); ER1(tacaatggcagcagcaagtc). Then re-amplify with the nested primer set: IL1(tcgcacattggtttgttgtt); IR1(aacggcatgagttagccaat). Mutagen: UV/TMP Outcrossed: x5 Made by: C Wolkow Received: 09/22/05 NIA ----------------------------------------------------------------------------- Strain: CY251 Species: Caenorhabditis elegans Genotype: sqt-1(sc13) age-1(mg44) II; bvIs2. Description: bvIs2 contains [ric-19p::age-1(cDNA)::myc::unc-54 3'UTR + mec-7::GFP]. sqt-1(sc13) causes recessive left-handed rollers. bvIs2 rescues mg44 dauer arrest and partially rescues mg44 longevity. Array can be detected by PCR (Fwd: 5'-GCA CAG TTT TAC GAA AGG AAC-3' / Rev: 5'-ACC ATC GTT TGC AGT TGT GG-3'). Reference: Iser WB, Gami MS, Wolkow CA. Dev Biol. 2007 Mar 15;303(2):434-47. Mutagen: Outcrossed: x1 Made by: Cathy Wolkow Received: 11/02/09 NIA ----------------------------------------------------------------------------- Strain: CY262 Species: Caenorhabditis elegans Genotype: sqt-1(sc13) age-1(mg44) II; bvIs1. Description: bvIs1 contains [ges-1p::age-1(cDNA)::unc-54 3'UTR + mec-7::GFP]. sqt-1(sc13) causes recessive left-handed rollers. bvIs1 rescues mg44 dauer arrest and partially rescues mg44 longevity. Array can be detected by PCR (Fwd: 5'-GTC ATT TTG GCA CCA TAG GAG-3' / Rev: 5'-ACC ATC GTT TGC AGT TGT GG-3'). Reference: Iser WB, Gami MS, Wolkow CA. Dev Biol. 2007 Mar 15;303(2):434-47. Mutagen: Outcrossed: x2 Made by: Cathy Wolkow Received: 11/02/09 NIA ----------------------------------------------------------------------------- Strain: CY397 Species: Caenorhabditis elegans Genotype: daf-16(mg242) I; sqt-1(sc13) age-1(mg109) II. Description: Sqt phenotype. The daf-16(mg242) allele is a dominant suppressor of age-1(mg109) daf-c phenotype. mg242 is a nonsense mutation Trp220Amb. Mutagen: EMS Outcrossed: x1 Made by: Minaxi S. Gami Received: 04/02/07 NIA ----------------------------------------------------------------------------- Strain: CY398 Species: Caenorhabditis elegans Genotype: daf-16(mg255) I; sqt-1(sc13) age-1(mg109) II. Description: Sqt phenotype. The daf-16(mg255) allele is a dominant suppressor of age-1(mg109) daf-c phenotype. mg255 is a nonsense mutation Try144Amb. Mutagen: EMS Outcrossed: x1 Made by: Minaxi S. Gami Received: 04/02/07 NIA ----------------------------------------------------------------------------- Strain: CY399 Species: Caenorhabditis elegans Genotype: sqt-1(sc13) age-1(mg109) II; pdk-1(mg261) X. Description: Sqt phenotype. The pdk-1(mg261) allele is a dominant suppressor of age-1(mg109) daf-c phenotype. mg261 is an activating missense mutation Ala447Val. Mutagen: EMS Outcrossed: x1 Made by: Minaxi S. Gami Received: 04/02/07 NIA ----------------------------------------------------------------------------- Strain: CY400 Species: Caenorhabditis elegans Genotype: sqt-1(sc13) age-1(mg109) II; akt-1(mg247) V. Description: Sqt phenotype. The akt-1(mg247) allele is a dominant suppressor of age-1(mg109) daf-c phenotype. mg247 is an activating missense mutation Ala149Thr. Mutagen: EMS Outcrossed: x2 Made by: Minaxi S. Gami Received: 04/02/07 NIA ----------------------------------------------------------------------------- Strain: CY401 Species: Caenorhabditis elegans Genotype: sqt-1(sc13) age-1(mg109)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUncs and Sqt. age-1(mg109) homozygotes throw all dauers at all temperatures (maternal effect dauer constitutive). age-1(mg109) homozygous animals that are maternally rescued for dauer formation are long-lived. Mutagen: EMS Outcrossed: x Made by: Ruvkun Lab Received: 04/02/07 NIA ----------------------------------------------------------------------------- Strain: CY573 Species: Caenorhabditis elegans Genotype: bvIs5. Description: bvIs5 [cyp-35B1p::GFP + gcy-7p::GFP]. Little or no GFP expression in adults. GFP expressed in intestine of dauers. Reference: Iser WB, et al. PLoS One. 2011 Mar 9;6(3):e17369. Mutagen: Outcrossed: x3 Made by: C Wolkow & W Iser Received: 03/30/11 NIA ----------------------------------------------------------------------------- Strain: CY577 Species: Caenorhabditis elegans Genotype: bvIs6. Description: bvIs6 [cat-4p::GFP + gcy-7p::GFP]. GFP localized to intestine, neurons and hypodermis in adults. GFP localized to neurons and seam cells in dauers. Reference: Iser WB, et al. PLoS One. 2011 Mar 9;6(3):e17369. Mutagen: Outcrossed: x3 Made by: C Wolkow & W Iser Received: 03/30/11 NIA ----------------------------------------------------------------------------- Strain: CYA1 Species: C. elegans Genotype: rexIs1. Description: rexIs1 [hsp-16p::halo::TEV::Keap1 + mec-7p::mRFP]. Constitutive red fluorescence in touch-receptor neurons. Heat-shock promoter drives expression of Halo protein with TEV recognition site for the tobacco etch virus protease and human Keap1 protein (an established RES sensor). No phenotypic change upon heat-shock (37°C). Integrated into N2 background; insertion site not known. Reference: Long MJC, et al. Biochemistry. 2017 Sep 12. doi: 10.1021/acs.biochem.7b00642. Mutagen: gamma irradiation Outcrossed: x2 Made by: Marcus J. C. Long Received: 12/20/17 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CYA10 Species: C. elegans Genotype: cyp-33E1(syb8844) IV; ldrIs1. Description: ldrIs1 [dhs-3p::dhs-3::GFP + unc-76(+)]. cyp-33E1(syb8844) is Cys439 mutated to Ala. dhs-3::GFP is expressed mainly in intestinal cells and localized to intestinal lipid droplets. Derived by crossing syb8844 with LIU1 and out-crossing with N2. Mutagen: Crispr/Cas9 Outcrossed: x6 Made by: Jinmin Liu Received: 09/20/24 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CYA11 Species: C. elegans Genotype: ldrIs1; eeeIs1. Description: ldrIs1 [dhs-3p::dhs-3::GFP + unc-76(+)]. eeeIs1 [unc-54p::Htt513(Q15)::YFP::unc-45 3'UTR]. Derived by crossing parental strains LIU1 and EAK102. YFP is fused to a fragment of mutant human Huntingtin protein; expression in body wall muscle cells. dhs-3::GFP is expressed mainly in intestinal cells and localized to intestinal lipid droplets. Mutagen: Outcrossed: x0 Made by: Jinmin Liu Received: 09/20/24 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CYA12 Species: C. elegans Genotype: ldrIs1; eeeIs2. Description: ldrIs1 [dhs-3p::dhs-3::GFP + unc-76(+)]. eeeIs2 [unc-54p::Htt513(Q128)::YFP::unc-54 3'UTR]. Motility defect. Derived by crossing parental strains LIU1 and EAK103. YFP is fused to a fragment of mutant human Huntingtin protein; expression in body wall muscle cells of the pharynx in adults and punctate expression in body wall muscle cells of larval animals. dhs-3::GFP is expressed mainly in intestinal cells and localized to intestinal lipid droplets. Mutagen: Outcrossed: x0 Made by: Jinmin Liu Received: 09/20/24 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CYA13 Species: C. elegans Genotype: frSi17 II; rde-1(ne300) V; ldrIs1. Description: frSi17 [mtl- 2p::rde-1 3'UTR] II. ldrIs1 [dhs-3p::dhs-3::GFP + unc-76(+)]. RDE-1 activity is rescued in the intestine, making animals RNAi-deficient except for intestinal tissues. Derived by crossing parental strains IG1839 and LIU1. dhs-3::GFP is expressed mainly in intestinal cells and localized to intestinal lipid droplets. frSi17 inserted into ttTi5605 site. Mutagen: Outcrossed: x0 Made by: Jinmin Liu Received: 09/20/24 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CYA14 Species: C. elegans Genotype: rde-1(ne300) V; neIs9 X; ldrIs1. Description: neIs9 [myo-3p::HA::rde-1 + rol-6(su1006)] X. ldrIs1 [dhs-3p::dhs-3::GFP + unc-76(+)]. Rollers. RDE-1 activity is rescued in the body-wall muscle, making animals RNAi-deficient except for body-wall muscle cells. Derived by crossing parental strains WM118 and LIU1. dhs-3::GFP is expressed mainly in intestinal cells and localized to intestinal lipid droplets. Mutagen: Outcrossed: x0 Made by: Jinmin Liu Received: 09/20/24 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CYA15 Species: C. elegans Genotype: rexEx7. Description: rexEx7 [gpa-4p::gfp::halo + mec-7p::mRFP]. Pick fluorescent worms to maintain. Constitutive red fluorescence in touch-receptor neurons. Green fluorescence in two ASI neurons. Mutagen: Outcrossed: x0 Made by: SunyBiotech Co., Ltd Received: 09/20/24 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CYA16 Species: C. elegans Genotype: rexEx8. Description: rexEx8 [sur-5p::GFP::halo + mec-7p::mRFP]. Pick fluorescent worms to maintain. Constitutive red fluorescence in touch-receptor neurons. Green fluorescence in somatic cells. Mutagen: Outcrossed: x0 Made by: SunyBiotech Co., Ltd Received: 09/20/24 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CYA17 Species: C. elegans Genotype: rexEx9. Description: rexEx9 [hlh-8p::GFP::halo + rol-6(su1006)]. Pick Rollers to maintain. GFP expression in M and undifferentiated cells of the M lineage. Mutagen: Outcrossed: x0 Made by: Daniel Urul Received: 09/20/24 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CYA18 Species: C. elegans Genotype: rexEx10. Description: rexEx10 [hsp-16p::his-6::Halo + mec-7p::mRFP]. Pick RFP+ to maintain. Constitutive red fluorescence in touch-receptor neurons. Heat shock induces the expression of Halo protein. Mutagen: Outcrossed: x0 Made by: Marcus J. C. Long Received: 09/20/24 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CYA19 Species: C. elegans Genotype: dvIs19 III; rexEx11. Description: dvIs19 [gst-4p::GFP::NLS] III. rexEx11 [hsp-16p::halo::TEV::Keap1 + mec-7p::mRFP]. Pick RFP+ worms to maintain. Constitutive red fluorescence in touch-receptor neurons. Heat shock induces expression of Halo::TEV::Keap1 protein. Oxidative stress induces expression of GFP. Superficially wild-type. Mutagen: Outcrossed: x0 Made by: Marcus J. C. Long Received: 09/20/24 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CYA2 Species: C. elegans Genotype: rexIs2. Description: rexIs2 [hsp-16p::tom70::mCherry::halo + mec-7p::mRFP]. Array is prone to silencing; pick RFP animals to maintain array. Constitutive red fluorescence in touch-receptor neurons. Heat-shock promoter drives expression of mCherry with tom70 (outer mitochondrial membrane targeting sequence from yeast) and Halo protein. Global red fluorescence upon heat-shock (37°C). Integrated into N2 background; insertion site not known. Reference: Long MJC, et al. Biochemistry. 2017 Sep 12. doi: 10.1021/acs.biochem.7b00642. Mutagen: gamma irradiation Outcrossed: x2 Made by: Marcus J. C. Long Received: 08/09/18 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CYA20 Species: C. elegans Genotype: dvIs19 III; rexEx12. Description: dvIs19 [gst-4p::GFP::NLS] III. rexEx12 [hsp-16p::tom70::mCherry::halo + mec-7p::mRFP]. Pick RFP+ worms to maintain. Constitutive red fluorescence in touch-receptor neurons. Heat shock induces expression of mCherry::Halo protein. Oxidative stress induces expression of GFP. Mutagen: Outcrossed: x0 Made by: Marcus J. C. Long Received: 09/20/24 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CYA21 Species: C. elegans Genotype: dvIs19 III; rexEx13. Description: dvIs19 [gst-4p::GFP::NLS] III. rexEx13 [hsp-16p::HA::wdr-23::halo + mec-7p::mRFP]. Pick RFP+ worms to maintain. Constitutive red fluorescence in touch-receptor neurons. Heat shock induces expression of HA::WDR23::Halo protein. Oxidative stress induces expression of GFP. Mutagen: Outcrossed: x0 Made by: Marcus J. C. Long Received: 09/20/24 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CYA22 Species: C. elegans Genotype: ahcy-1(syb748) I. Description: ahcy-1(syb748) is a CRISPR/Cas9-engineered C280A substitution that eliminates electrophile sensing but retains enzymatic activity. Mutagen: CRISPER/CAS9 Outcrossed: x6 Made by: SunyBiotech Co., Ltd Received: 09/20/24 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CYA23 Species: C. elegans Genotype: ahcy-1(syb748) I; sqIs13. Description: sqIs13 [lgg-1p::GFP::lgg-1 + odr-1p::RFP]. Pick RFP+/GFP+ animals to maintain. GFP fluorescence in autophagosomes. RFP fluorescence in AWB and AWC. ahcy-1(syb748) is a CRISPR/Cas9-engineered C280A substitution that eliminates electrophile sensing but retains enzymatic activity. Mutagen: Outcrossed: x5 Made by: Yong-Qi Gao Received: 09/20/24 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CYA3 Species: C. elegans Genotype: rexEx1. Description: rexEx1 [myo-2p::GFP::Halo + mec-7p::mRFP]. Pick GFP+ to maintain. Mosaic expression of green fluorescence (GFP) in pharynx and red fluorescence (mRFP) in touch-receptor neurons. Mutagen: Outcrossed: x0 Made by: SunyBiotech Co., Ltd Received: 09/20/24 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CYA4 Species: C. elegans Genotype: rexEx2. Description: rexEx2 [ges-1p::GFP::Halo + mec-7p::mRFP]. Pick GFP+ to maintain. Mosaic expression of green fluorescence (GFP) in the intestine and red fluorescence (mRFP) in touch-receptor neurons. Mutagen: Outcrossed: x0 Made by: SunyBiotech Co., Ltd Received: 09/20/24 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CYA5 Species: C. elegans Genotype: rexEx3. Description: rexEx3 [myo-3p::GFP::Halo + mec-7p::mRFP]. Pick GFP+ to maintain. Mosaic expression of green fluorescence (GFP) in body-wall muscle and red fluorescence (mRFP) in touch-receptor neurons. Mutagen: Outcrossed: x0 Made by: SunyBiotech Co., Ltd Received: 09/20/24 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CYA6 Species: C. elegans Genotype: rexEx4. Description: rexEx4 [myo-2p::mCherry::P2A::Flag::UltraID::unc-54 3'UTR]. Pick mCherry+ to maintain. Mosaic expression of red fluorescence (mCherry) in pharynx. P2A is the self-cleaving peptide sequence. Mutagen: Outcrossed: x0 Made by: SunyBiotech Co., Ltd Received: 09/20/24 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CYA7 Species: C. elegans Genotype: rexEx5. Description: rexEx5 [ges-1p::mCherry::P2A::Flag::UltraID::unc-54 3'UTR]. Pick mCherry+ to maintain. Mosaic expression of red fluorescence (mCherry) in the intestine. P2A is the self-cleaving peptide sequence. Mutagen: Outcrossed: x0 Made by: SunyBiotech Co., Ltd Received: 09/20/24 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CYA8 Species: C. elegans Genotype: rexEx6. Description: rexEx6 [myo-3p::mCherry::P2A::Flag::UltraID::unc-54 3'UTR]. Pick mCherry+ to maintain. Mosaic expression of red fluorescence (mCherry) in body-wall muscle; punctate mCherry signals in some animals. P2A is the self-cleaving peptide sequence. Mutagen: Outcrossed: x0 Made by: SunyBiotech Co., Ltd Received: 09/20/24 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CYA9 Species: C. elegans Genotype: cyp-33E1(syb8800) IV; ldrIs1. Description: ldrIs1 [dhs-3p::dhs-3::GFP + unc-76(+)]. cyp-33E1(syb8800) is Cys295 mutated to Ala. dhs-3::GFP is expressed mainly in intestinal cells and localized to intestinal lipid droplets. Derived by crossing syb8800 with LIU1 and out-crossing with N2. Mutagen: Crispr/Cas9 Outcrossed: x6 Made by: Jinmin Liu Received: 09/20/24 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: CZ10123 Species: C. elegans Genotype: rabx-5(qa7800) III. Description: rabx-5(qa7800) mutants show decreased protein localization of YFP::RAB-5 in the cell bodies but increased protein localization within the dorsal cord in both synaptic and intersynaptic regions Mutagen: Outcrossed: x1 Made by: Sharon Sann Received: 07/11/17 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ10175 Species: Caenorhabditis elegans Genotype: zdIs5 I. Description: zdIs5 [mec-4p::GFP + lin-15(+)] I. SK4005 was outcrossed 10x to produce this strain. Mutagen: Outcrossed: x12 Made by: Anindya Ghosh Roy Received: 10/19/11 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ1072 Species: Caenorhabditis elegans Genotype: unc-62(e917) V. Description: Inversion which may serve as a balancer for the center of LG V. Maternal effect lethal allele which results in 57% embryonic arrest, 40% larval arrest, and 3% which survive to be fertile adults with a variety of defects including Egl, Unc and Vab. Mutagen: P32 Outcrossed: x Made by: A Chisholm Received: 12/22/03 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: CZ11771 Species: Caenorhabditis elegans Genotype: nsf-1(ty10) I; muIs32 II. Description: muIs32 [mec-7p::GFP + lin-15(+)]. Egl, semi-Sterile, embryonic & larval lethality. Lethal in trans to Df. Defective fusion of anchor cell to uterine seam. Maintain under normal conditions. Reference: Choi J, et al., Dev Biol. 2006 Sep 1;297(1):87-102. Mutagen: Outcrossed: x0 Made by: Anindya Ghosh Roy Received: 12/19/11 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ1251 Species: Caenorhabditis elegans Genotype: rpm-1(ju41) V. Description: WT in movement. Abnormal synapses at GABAergic NMJs. Mutagen: EMS Outcrossed: x>3 Made by: Yishi Jin Received: 07/31/00 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ13799 Species: C. elegans Genotype: juIs76 II. Description: juIs76 [unc-25p::GFP + lin-15(+)] II. GFP expression in GABAergic motor neurons. [NOTE: (10/11/2018) CZ13799 was sent as a replacement for CZ1200, which was found to carry background mutations affecting neuron morphology.] Derived by outcrossing CZ1200 to remove lin-15(n765) and unidentified background mutations. Reference (for original juIs76 strain): Huang X, et al. Neuron. 2002 May 16;34(4):563-76. Mutagen: Outcrossed: x5 Made by: Jason McEwen Received: 10/24/18 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ13896 Species: Caenorhabditis elegans Genotype: juIs319. Description: juIs319 [col-19p::GCaMP3 + col-19p::tdTomato]. col-19p::GCaMP3 expression is induced by either laser or needle wounding. col-19 is an adult-specific collagen and is not expressed until the end of the L4 larval stage. Reference: Xu S & Chisholm AD. Curr Biol. 2011 Dec 6;21(23):1960-7. Mutagen: UV Outcrossed: x6 Made by: Suhong Xu Received: 05/30/14 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ1455 Species: Caenorhabditis elegans Genotype: cnd-1(ju29) III. Description: Reverse kinker. Mutagen: EMS Outcrossed: x>4 Made by: Steven Hallam Received: 02/20/01 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ14748 Species: Caenorhabditis elegans Genotype: juIs352. Description: juIs352 [col-19p::GFP::moesin]. GFP::moesin expression labels F-actin by fusing GFP to sequences that encoded the C-terminal end of the sole Drosophila MER homolog, moesin. The F-actin ring (GFP::moesin) is induced upon needle wounding. Reference: Xu S & Chisholm AD. Curr Biol. 2011 Dec 6;21(23):1960-7. Mutagen: UV Outcrossed: x6 Made by: Suhong Xu Received: 05/30/14 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ1566 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) juIs109 X. Description: juIs109 [efn-4::GFP + lin-15(+)] X. Superficailly wild-type. GFP expression detected under high power in a subset of head neurons, primary vulval cells, and a pair of pharyngeal neurons. Reference: Chin-Sang ID, et al. Development. 2002 Dec;129(23):5499-510. Mutagen: X-ray Outcrossed: x1 Made by: Sean George Received: 05/09/11 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ16143 Species: C. elegans Genotype: juEx4586 [rgef-1p::GFP1-10 + ttx-3p::RFP]. Description: juEx4586 [rgef-1p::GFP1-10 + ttx-3p::RFP]. Pick RFP+ to maintain. RFP expression in AIY neuron. Weak light green signals were observed in the neurons using compound scope although this is only the former half of the split GFP. Reference: Noma K, et al. Elife. 2017 Aug 2;6:e26376. doi: 10.7554/eLife.26376. Mutagen: Outcrossed: x0 Made by: Ken Noma Received: 06/25/21 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ1632 Species: Caenorhabditis elegans Genotype: juIs76 II; max-1(ju39) V. Description: juIs76 [unc-25p::GFP + lin-15(+)] II. Very mild Unc. Mutagen: EMS Outcrossed: x3 Made by: X Huang & Y Jin Received: 11/18/02 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ16518 Species: C. elegans Genotype: juEx4796. Description: juEx4796 [col-19p::mito::GFP + ttx-3p::RFP]. Pick animals with red fluorescence to maintain array. Transgenic animals express mito::GFP in the hypodermis. Reference: Fu H, et al. Nat Commun. 2020 Feb 26;11(1):1050. PMID: 32103012. Mutagen: Outcrossed: x0 Made by: Suhong Xu Received: 11/15/22 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ17515 Species: C. elegans Genotype: juSi94 II; rps-18(ok3353) IV. Description: juSi94 [GFP11::rps-18 + Cbr-unc-119(+)] II. Superficially wild-type. No fluorescence; carries only one portion of a split GFP reporter for visualization of ribosomes. Allows inducible GFP fluorescence of ribosomes when combined with GFP1-10 expression in tissue of choice. Reference: Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 10.7554/eLife.26376. Mutagen: Outcrossed: x0 Made by: Ken Noma Received: 09/07/17 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ1758 Species: Caenorhabditis elegans Genotype: juIs76 II; max-1(ju142) V. Description: juIs76 [unc-25p::GFP + lin-15(+)] II. Very mild Unc. Mutagen: EMS Outcrossed: x1 Made by: X Huang & Y Jin Received: 11/18/02 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ1774 Species: C. elegans Genotype: vab-1(e856) ptp-3(op147)/mIn1 [mIs14 dpy-10(e128)] II. Description: mIs14 [myo-2::GFP + pes-10::GFP]. Homozygous lethal double mutant balanced by GFP- and dpy-10-marked inversion. Pick wild-type GFP+ to maintain. Heterozygotes are wild-type with pharyngeal GFP signal, and segregate wild-type GFP+, Dpy bright GFP+ (mIn1 homozygotes), and non-GFP homozygous vab-1 ptp-1 double mutants. vab-1 ptp-1 double mutants (non-GFP) are embryonic lethal. Reference: Harrington RJ, et al. Development. 2002 May;129(9):2141-53. Mutagen: Outcrossed: x0 Made by: Andrew Chisholm Received: 04/02/18 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ18018 Species: C. elegans Genotype: juSi94 II; rps-18(ok3353) IV; juEx5375. Description: juSi94 [rps-18p::GFP11::rps-18 + Cbr-unc-119(+)] II. juEx5375 [col-19p::GFP1-10 + ttx-3p::RFP]. Pick RFP+ to maintain. Expression of split GFP reporter labels ribosomes in the epidermis. Reference: Noma K, et al. Elife. 2017 Aug 2;6:e26376. doi: 10.7554/eLife.26376. Mutagen: MosSCI Outcrossed: x0 Made by: Ken Noma Received: 12/03/20 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ18020 Species: C. elegans Genotype: juSi94 II; rps-18(ok3353) IV; juEx5377. Description: juSi94 [GFP11::rps-18 + Cbr-unc-119(+)] II. juEx5377 [myo-3p::GFP1-10 + ttx-3p::RFP]. Pick ttx-3::RFP to maintain. Muscle-specific expression of split GFP reporter allows visualization of ribosomes in muscle. Reference: Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 10.7554/eLife.26376. Mutagen: Outcrossed: x0 Made by: Ken Noma Received: 09/07/17 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ18058 Species: C. elegans Genotype: juSi103. Description: juSi103 [col-19p::mito::GCaMP5]. GCaMP5 reporter labels hypodermal mitochondria. Reference: Xu S & Chisholm AD. Dev Cell. 2014 Oct 13;31(1):48-60. doi: 10.1016/j.devcel.2014.08.002. PMID: 25313960. Mutagen: Outcrossed: x0 Made by: Suhong Xu Received: 12/16/21 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ18401 Species: C. elegans Genotype: rpl-29(tm3555) IV. Description: Homozygous viable Reference: Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 10.7554/eLife.26376. Mutagen: Outcrossed: x4 Made by: Kentaro Noma Received: 05/04/21 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ18412 Species: C. elegans Genotype: juSi94 II; rps-18(ok3353) IV; glo-4(ok623) V; juEx5515. Description: juSi94 [GFP11::rps-18 + Cbr-unc-119(+)] II. juEx5515 [unc-25p::GFP1-10 + unc-25p::mCherry::rab-3 + ttx-3p::RFP]. Pick ttx-3::RFP to maintain. GABAergic motor neuron-specific expression of split GFP reporter allows visualization of ribosomes in neurons, and GABAergic motor neuron-specific expression of mCherry::rab-3. Reference: Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 10.7554/eLife.26376. Mutagen: Outcrossed: x0 Made by: Ken Noma Received: 09/07/17 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ18423 Species: C. elegans Genotype: juEx5375. Description: juEx5375 [col-19p::GFP1-10 + ttx-3p::RFP]. Pick RFP+ to maintain. Reference: Noma K, et al. Elife. 2017 Aug 2;6:e26376. doi: 10.7554/eLife.26376. Mutagen: Outcrossed: x1 Made by: Ken Noma Received: 12/03/20 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ18550 Species: C. elegans Genotype: juSi123 II; rpl-29(tm3555) IV. Description: juSi123 [rpl-29::GFP] II. Strong GFP signal allowing visualization of ribosomes. GFP inserted at C-terminus of RPL-29. Reference: Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 10.7554/eLife.26376. Mutagen: Outcrossed: x0 Made by: Ken Noma Received: 09/07/17 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ18637 Species: C. elegans Genotype: juSi83 II; rps-18(ok3353) IV/nT1[qIs51] (IV;V). Description: juSi83 [GFP::rps-18 + Cbr-unc-119(+)] II. Homozygous lethal mutation balanced by GFP-marked translocation. Heterozygotes are WT GFP+ and segregate WT GFP+, Vul and dead eggs. Non-conditonal GFP-tagged ribosomes; array over-expressing N-terminally tagged rps-18 (small ribosomal subunit) partially rescues rps-18(ok3353) larval arrest (some animals will escape L1 arrest and develop to L3 stage). Reference: Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 10.7554/eLife.26376. Mutagen: Outcrossed: x0 Made by: Ken Noma Received: 09/07/17 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ1893 Species: Caenorhabditis elegans Genotype: syd-1(ju82) II. Description: Egl. Coiler when moving backwards. Recessive. F35D2.5 Mutagen: EMS Outcrossed: x>2 Made by: Yishi Jin Received: 01/27/03 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ18975 Species: Caenorhabditis elegans Genotype: juIs338. Description: juIs338 [mec-4p::ebp-2::GFP + ttx-3p::RFP]. Derived by integration of array in parental strain CZ12264. Reference: Ghosh-Roy A, et al. Dev Cell. 2012 Oct 16;23(4):716-28. Chen L, et al. Elife. 2015 Sep 4;4. doi: 10.7554/eLife.08695. Mutagen: TMP/UV Outcrossed: x7 Made by: Anindya Ghosh Roy Received: 04/19/17 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ19297 Species: C. elegans Genotype: juSi94 II; rps-18(ok3353) juIs409 IV. Description: juSi94 [GFP11::rps-18 + Cbr-unc-119(+)] II. juIs409 [rgef-1p::GFP1-10 + ttx-3p::RFP] IV. Pan-neuronal-specific expression of split GFP reporter allows visualization of ribosomes in neurons. Reference: Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 10.7554/eLife.26376. Mutagen: UV/TMP Outcrossed: x0 Made by: Ken Noma Received: 09/07/17 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ19299 Species: C. elegans Genotype: juSi94 juIs438 II; rps-18(ok3353) IV. Description: juIs438 [mec-4p::GFP1-10 + mec-4p::tagRFP] II. juSi94 [rps-18p::GFP11::rps-18]. Expression of split GFP reporter labels ribosomes in touch neurons. Generated in N2 background. Reference: Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 10.7554/eLife.26376. Mutagen: Outcrossed: x0 Made by: Kentaro Noma Received: 01/26/21 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ1931 Species: Caenorhabditis elegans Genotype: juIs76 II; unc-71(ju156) III. Description: juIs76 [unc-25p::GFP + lin-15(+)] II. Unc. Mutagen: EMS Outcrossed: x3 Made by: X Huang & Y Jin Received: 11/18/02 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ1935 Species: Caenorhabditis elegans Genotype: juIs76 II; unc-71(ju160) III. Description: juIs76 [unc-25p::GFP + lin-15(+)] II. Unc. Mutagen: EMS Outcrossed: x3 Made by: X Huang & Y Jin Received: 11/18/02 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ19982 Species: Caenorhabditis elegans Genotype: mcu-1(ju1154) IV. Description: Superficially wild-type. No uptake of calcium in mitochondria after wounding. Reference: Xu S, Chisholm AD. Dev Cell. 2014 Oct 13; 31:48-60. Mutagen: CRISPR-Cas9 Outcrossed: x2 Made by: Suhong Xu Received: 12/15/14 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ20132 Species: C. elegans Genotype: juSi94 II; rps-18(ok3353) IV; juIs463. Description: juSi94 [GFP11::rps-18 + Cbr-unc-119(+)] II. juIs463 [flp-13p::GFP1-10 + ttx-3p::RFP]. DD motor neuron-specific expression of split GFP reporter allows visualization of ribosomes in those neurons. Reference: Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 10.7554/eLife.26376. Mutagen: UV/TMP Outcrossed: x0 Made by: Ken Noma Received: 09/07/17 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ20215 Species: Caenorhabditis elegans Genotype: tba-1(ju89) I. Description: Gain-of-function allele isolated sem-4; juIs1 screen in 1997. ju89 has slightly semi-dominant effects, and the gf phenotype is most obvious in homozygous. This outcrossed strain does not carry a sem-4 mutation or juIs1. References: Kurup N, et al. Curr Biol. 2015 Jun 15;25(12):1594-605. Kurup N, et al. PLoS Genet. 2017 Jun 21;13(6):e1006844. Mutagen: EMS Outcrossed: x2 Made by: Naina Kurup Received: 01/09/18 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ20310 Species: Caenorhabditis elegans Genotype: juSi164 unc-119(ed3) III. Description: juSi164 [mex-5p::HIS-72::miniSOG + Cbr-unc-119(+)] III. Maintain in the covered box to avoid unnecessary exposure to ambient light. Wild-type behavior in movement, mating, growth and brood size. Upon blue light treatment (460 nm LED light for 30 min at 4 Hz with 2 mW/mm2), Histone-miniSOG in the germline can induce heritable mutations. Wild-type behavior in movement, mating, growth and brood size. MosSCI insertion into oxTi444 on Chr. III. Reference: Noma K & Jin Y. Nature Communications, 2015. Mutagen: MosSCI Outcrossed: x0 Made by: Kentaro Noma Received: 11/10/15 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ20473 Species: C. elegans Genotype: ncs-2(ju836) I. Description: Superficially wild-type. Reference: Zhou K, et al. Cell Reports 2017 May 9;19(6):1117-1129. PMID: 28494862 Mutagen: Outcrossed: x2 Made by: Keming Zhou Received: 05/04/21 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ2060 Species: C. elegans Genotype: juIs137 II. Description: juIs137 [flp-13p::snb-1::GFP] II. GFP expression labels synaptic vesicles. Reference: Sakaguchi-Nakashima A, et al. Curr Biol. 2007 Apr 3;17(7):592-8. doi: 10.1016/j.cub.2007.01.074. PMID: 17346966. Mutagen: Outcrossed: x6 Made by: James Meir Received: 02/28/25 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ22190 Species: C. elegans Genotype: rpm-1(ok364) rpms-1(ju1285) V. Description: ju1285 is a deletion in F07B7.12/rpms-1. Reference: Sun Y, et al. MicroPubl Biol. 2024 Dec 5;2024:10.17912/micropub.biology.001396. doi: 10.17912/micropub.biology.001396. PMID: 39712931. Mutagen: Outcrossed: x2X Made by: Daniela Gaio Received: 02/28/25 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ22695 Species: Caenorhabditis elegans Genotype: juEx6908. Description: juEx6908 [nmr-1p::PH::miniSOG(Q103L) + nmr-1p::mCherry + ttx-3::GFP]. Pick GFP+ to maintain. Interneuron expression of mCherry and PH-miniSOG (mini Singlet Oxygen Generator). Reference: Xu S & Chisholm AD. Sci Rep. 2016 Feb 10;6:21271. Mutagen: Outcrossed: x0 Made by: Suhong Xu Received: 07/27/16 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ22698 Species: Caenorhabditis elegans Genotype: juEx6911. Description: juEx6911 [unc-25p::PH::miniSOG(Q103L) + unc-25p::mCherry + ttx-3::GFP]. Pick GFP+ to maintain. Expression of mCherry and PH-miniSOG (mini Singlet Oxygen Generator) in GABAergic motor neurons. Reference: Xu S & Chisholm AD. Sci Rep. 2016 Feb 10;6:21271. Mutagen: Outcrossed: x0 Made by: Suhong Xu Received: 07/27/16 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ22703 Species: Caenorhabditis elegans Genotype: juEx6916. Description: juEx6916 [myo-3p::PH::miniSOG(Q103L) + myo-3p::mCherry + ttx-3::GFP]. Pick GFP+ to maintain. Expression of mCherry and PH-miniSOG (mini Singlet Oxygen Generator) in body wall muscles. Reference: Xu S & Chisholm AD. Sci Rep. 2016 Feb 10;6:21271. Mutagen: Outcrossed: x0 Made by: Suhong Xu Received: 07/27/16 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ22714 Species: C. elegans Genotype: miro-3(ju1310) juSi271 I; miro-1(ju1306) IV; miro-2(ju1309) X. Description: juSi271 [col-19p::mito::Dendra2]. Superficially wild-type with altered mitochondrial morphology. Fluorescent reporter labels hypodermal mitochondria. Reference: Xu S, et al. J Genet Genomics. 2016 Feb 20;43(2):103-6. Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Suhong Xu Received: 11/25/19 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ2274 Species: Caenorhabditis elegans Genotype: efn-4(bx80) efn-2(ev658) IV; efn-3(ev696) X. Description: bx80 was previously called mab-26(bx80): Extensive ray fusion involving all 9 rays; Larva have Vab phenotype with decreasing expressivity in adult; Hermaphrodites have swollen tail and anus. Vab, embryonic ventral enclosure defects, male ray fusions. Slow growth. Mutagen: Outcrossed: x Made by: Received: 12/10/07 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CZ23277 Species: Caenorhabditis elegans Genotype: juEx7101. Description: juEx7101 [col-19p::PH::miniSOG(Q103L) + ttx-3::RFP]. Pick RFP+ to maintain. Adult epidermal expression of PH-miniSOG (mini Singlet Oxygen Generator). Reference: Xu S & Chisholm AD. Sci Rep. 2016 Feb 10;6:21271. Mutagen: Outcrossed: x0 Made by: Suhong Xu Received: 07/27/16 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ23279 Species: Caenorhabditis elegans Genotype: juEx7103. Description: juEx7103 [unc-17p(beta)::PH::miniSOG(Q103L) + acr-2p::mCherry + ttx-3::RFP]. Pick RFP+ to maintain. Expression of mCherry and PH-miniSOG (mini Singlet Oxygen Generator) in cholinergic motor neurons. Reference: Xu S & Chisholm AD. Sci Rep. 2016 Feb 10;6:21271. [NOTE: strain was previously described as carrying ttx-3::GFP, but appears to be ttx-3::RFP instead.] Mutagen: Outcrossed: x0 Made by: Suhong Xu Received: 07/27/16 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ23281 Species: Caenorhabditis elegans Genotype: juEx7105. Description: juEx7105 [mec-4p::PH::miniSOG(Q103L) + mec-4p::mCherry + ttx-3::RFP]. Pick RFP+ to maintain. Expression of mCherry and PH-miniSOG (mini Singlet Oxygen Generator) in mechanosensory neurons. Reference: Xu S & Chisholm AD. Sci Rep. 2016 Feb 10;6:21271. Mutagen: Outcrossed: x0 Made by: Suhong Xu Received: 07/27/16 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ23908 Species: C. elegans Genotype: rab-8(tm2526) I; muIs32 II. Description: muIs32 [mec-7p::GFP + lin-15(+)]. Homozygous viable. Reference: Kim KW, et al. Elife. 2018 Nov 21;7:e39756. doi: 10.7554/eLife.39756. PMID: 30461420 Mutagen: Outcrossed: x4X Made by: Laura Toy Received: 05/04/21 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ24092 Species: C. elegans Genotype: gip-2(lt19[gip-2::GFP::loxP::Cbr-unc-119(+)::loxP]) I; ltSi953 II; unc-119(ed3) III. Description: ltSi953 [mec-18p::vhhGFP4::ZIF-1::operon-linker::mKate2::tbb-2 3'UTR + Cbr-unc-119(+)] II. GFP tag inserted into the C-terminus of the endogenous gip-2 locus using CRISPR-Cas9 engineering. Tissue-specific expression of GFP nanobody::ZIF-1 fusion promotes ubiquitylation and subsequent degradation of GFP-tagged gip-2 protein in touch receptor neurons. Touch receptor neurons are red labeled with mKate2. Reference: Development. 2017 Jul 15;144(14):2694-2701. PMID: 28619826. Mutagen: Outcrossed: x0 Made by: Ngang Heok Tang Received: 05/06/22 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ24274 Species: C. elegans Genotype: dhc-1(lt45[dhc-1::GFP]) I; ltSi953 II; unc-119(ed3) III. Description: ltSi953 [mec-18p::vhhGFP4::ZIF-1::operon-linker::mKate2::tbb-2 3'UTR + Cbr-unc-119(+)] II. GFP tag inserted into the C-terminus of the endogenous dhc-1 locus using CRISPR-Cas9 engineering. Tissue-specific expression of GFP nanobody::ZIF-1 fusion promotes ubiquitylation and subsequent degradation of GFP-tagged dhc-1 protein in touch receptor neurons. Touch receptor neurons are red labeled with mKate2. Reference: Development. 2017 Jul 15;144(14):2694-2701. PMID: 28619826. Mutagen: Outcrossed: x0 Made by: Ngang Heok Tang Received: 05/06/22 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ2475 Species: C. elegans Genotype: juIs145 II. Description: juIs145 [flp-13p::GFP + lin-15(+)] II. GFP expression in ASE, ASG, ASK, I5 and DD1-DD6. Reference: Wu Z, et al. Proc Natl Acad Sci USA. 2007 Sep 18;104(38):15132-7. PMID: 17848506. Mutagen: UV/TMP Outcrossed: x6 Made by: James Meir Received: 05/06/22 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ2485 Species: Caenorhabditis elegans Genotype: ahr-1(ju145) I. Description: Mutagen: EMS Outcrossed: x>4 Made by: Xun Huang Received: 05/03/04 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ24969 Species: C. elegans Genotype: sphk-1(ju831) II. Description: Aldicarb resistant. Reference: McCulloch KA, et al. G3 (Bethesda). 2017 Jul 5;7(7):2055-2063. Mutagen: EMS Outcrossed: x2 Made by: Kate McCulloch Received: 02/21/18 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ24990 Species: C. elegans Genotype: unc-44(ju1412[unc-44::GFP]) IV. Description: Endogenous unc-44 locus tagged for UNC-44L::GFP expression. The long isoform of UNC-44 is specific to neuronal expression. Reference: Chen F, Chisholm AD, Jin Y. Development. 2017 Feb 15;144(4):698-707. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Fei Chen Received: 06/18/18 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ25013 Species: C. elegans Genotype: unc-44(ju1413[unc-44::gfp::LoxP::3xflag]) IV. Description: unc-44(ju1413[unc-44::GFP::LoxP::3xflag]) IV. UNC-44C (short isoform of UNC-44) tagged with GFP. UNC-44C is strongly expressed in multiple tissues: nervous system (from 1.5-fold stage to adult), epidermis (from early embryo to adult), seam cells (from L1 to L4), vulva (from L3 to adult), and spermatheca/sheath cells (from L4 to adult). Reference: Chen F, Chisholm AD, Jin Y. Development. 2017 Feb 15;144(4):698-707. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Fei Chen Received: 06/18/18 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ252 Species: Caenorhabditis elegans Genotype: itr-1(n2559)/lin-45(n2018) unc-24(e138) IV. Description: Heterozygotes are WT and segregate WT, Unc Lins and very slow growing constipated worms. n2559 is a null allele of itr-1. Mutagen: Outcrossed: x Made by: Andrew Chisholm Received: 06/15/01 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ25415 Species: C. elegans Genotype: nmat-2(ju1514) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); muIs32 II. Description: muIs32 [mec-7p::GFP + lin-15(+)]. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP nmat-2(ju1514) homozygotes (sterile adults). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Kim KW, et al. Elife. 2018 Nov 21;7:e39756. doi: 10.7554/eLife.39756. PMID: 30461420 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Kyung Won Kim Received: 08/18/22 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ25643 Species: C. elegans Genotype: nmat-1(ju1565) X. Description: Reference: Kim KW, et al. Elife. 2018 Nov 21;7:e39756. doi: 10.7554/eLife.39756. PMID: 30461420 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Kyung Won Kim Received: 08/18/22 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ25708 Species: C. elegans Genotype: prg-1(ju1574) I. Description: Temperature sensitive sterility: maintain at 15-20C. prg-1(ju1574) mutant animals become sterile at the fifth generation grown at 25C. prg-1(ju1574) contains two mutations in the PIWI domain active site (RNaseH/slicer) [D583A, Y585A]. Mutation of the first conserved aspartate of the catalytic triad (D-D-H motif) to alanine (D583A) created an A-D-H motif which abolishes slicer activity in Argonaute proteins. WT: [GTCGGCTACGATCTGTACCACGACTCGACATTGAAAGGAAAAACT --> VGYDLYHDSTLKGKT] ju1574: [GTCGGCTACGcgCTGgctCAtGAtTCGACATTGAAAGGAAAAACT --> CGYALAHDSTLKGKT] Forward genotyping primer: GTAATGCTCGCTGACGACAA Reverse genotyping primer: TTGACGAACTGTGGAACCAA Reference: Kim KW, et al. Neuron. 2018 Feb 7;97(3):511-519.e6. doi: 10.1016/j.neuron.2018.01.014. Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Kyung Won Kim Received: 09/12/19 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ25941 Species: C. elegans Genotype: dlk-1(ju1579[gfp::dlk-1]) I. Description: Endogenous dlk-1 locus tagged with GFP using CRISPR/Cas9. GFP is not visible under compound fluorescence microscope. Reference: Sun Y, et al. Proc Natl Acad Sci U S A. 2023 Sep 26;120(39):e2302801120. doi: 10.1073/pnas.2302801120. PMID: 37722038. Mutagen: Outcrossed: x2 Made by: Yue Sun Received: 02/28/25 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ2611 Species: Caenorhabditis elegans Genotype: vab-2(ju1) efn-2(ev658) IV; efn-3(ev696) X. Description: vab-2(ju1) has embryonic lethality (12%) and notched heads (about 40%). vab-2(ju1) is considered a null allele (W30opal), and was previously called efn-1. Vab, embryonic ventral enclosure defects, male ray fusions. Mutagen: Outcrossed: x Made by: Received: 12/10/07 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: CZ26389 Species: C. elegans Genotype: esyt-2(ju1408) III. Description: CRISPR-engineered deletion of esyt-2 from middle of 5'UTR to middle of 3'UTR using guide RNAs crCP01 (GGTTTCAGTAATTGTGGGCT) and crCP02 (GTGCACTTACGGGTTGTAGG). Superficially wild-type. Reference: Piggott CA, et al. Genetics. 2021 Apr 19;iyab063. doi: 10.1093/genetics/iyab063. PMID: 33871019. Mutagen: Crispr/Cas9 Outcrossed: x4 Made by: Christopher Piggott Received: 12/16/21 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ26494 Species: C. elegans Genotype: juSi364 IV; acr-2(n2420) X. Description: juSi364 [unc-17Bp::3xFLAG::eif-3.g::SL2::GFP] IV. GFP expression in cholinergic motor neurons. Convulsing worms. Strain is suitable for neuron-type eCLIP. Reference: Blazie S, et al. Elife. 2021 Jul 29;10:e68336. PMID: 34323215. Mutagen: Outcrossed: x1 Made by: Steve Blazie Received: 02/02/22 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ26606 Species: C. elegans Genotype: vwa-8(ju1659) X. Description: Superficially wild-type. Reference: Zhu, M, et al. A null mutation of C. elegans vwa-8. microPublication Biology. https://doi.org/10.17912/micropub.biology.000263 Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Ming Zhu Received: 06/29/20 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ26660 Species: C elegans Genotype: micu-1(ju1155) IV/nT1 [qIs51] (IV;V). Description: Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP micu-1 homozygotes. Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Tang NH, et al. Curr Biol. 2020 Jan 15. pii: S0960-9822(19)31694-X. doi: 10.1016/j.cub.2019.12.061. Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Ngang Heok Tang Received: 03/18/20 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ27190 Species: C. elegans Genotype: juIs550. Description: juIs550 [mec-4p::mito::GCaMP5 + ttx-3p::RFP]. Mitochondrial calcium reporter expressed in touch neurons. Reference: Tang NH, et al. Curr Biol. 2020 Mar 9;30(5):865-876.e7. doi: 10.1016/j.cub.2019.12.061. PMID: 31983639 Mutagen: UV-TMP Outcrossed: x2 Made by: NgangHeok Tang Received: 06/20/23 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ2744 Species: Caenorhabditis elegans Genotype: adm-4(ok265) X. Description: Wild type. Mutagen: UV/TMP Outcrossed: x0 Made by: X Huang/Y Jin Received: 10/21/02 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ27508 Species: C elegans Genotype: micu-1(ju1783[micu-1::gfp]) IV. Description: GFP reporter inserted into C-terminus of endogenous micu-1 locus. Diffuse GFP signals throughout the body. Reference: Tang NH, et al. Curr Biol. 2020 Jan 15. pii: S0960-9822(19)31694-X. doi: 10.1016/j.cub.2019.12.061. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Ngang Heok Tang Received: 03/18/20 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ27593 Species: C. elegans Genotype: bli-1(ju1789[bli-1::mNG::3xFLAG]) II. Description: Superficially wild type with green fluorescence in L4 epidermis and adult stage cuticle. mNeonGreen and 3xFLAG tags inserted in N-terminus of endogenous BLI-1 locus at A106 (after subtilisin cleavage site) using Dickinson method. Reference: Adams JRG, et al. Nat Commun. 2023 Nov 18;14(1):7506. doi: 10.1038/s41467-023-43058-9. PMID: 37980413. Mutagen: Outcrossed: x0 Made by: Jennifer Gotenstein Adams Received: 12/01/23 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ27748 Species: C. elegans Genotype: vwa-8(ju1799[vwa-8::GFP::3xFLAG]) X. Description: Endogenous vwa-8 locus tagged with GFP and 3xFLAG. VWA-8::GFP is expressed in mitochondria of hypodermis, intestine, and muscle, but not detectable in neurons. Reference: Zhu, M, et al. A null mutation of C. elegans vwa-8. microPublication Biology. https://doi.org/10.17912/micropub.biology.000263. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Ming Zhu Received: 06/29/20 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ28766 Species: C. elegans Genotype: col-19::mNG(syb4625) X. Description: mNG inserted at C-terminus of endogenous col-19 locus. Derived by out-crossing parental strain PHX4625 2x to N2. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: SunyBiotech Received: 12/04/24 SunyBiotech Co., Ltd ----------------------------------------------------------------------------- Strain: CZ29001 Species: C. elegans Genotype: muIs32 II; degt-1(ok3307) V. Description: muIs32 [mec-7p::GFP + lin-15(+)]. GFP-labeled touch receptor neurons showing wild-type-like morphology. Derived by out-crossing parental ok3307 strain to remove a linked mutation in rpm-1. Reference: Jin EJ & Jin Y. (2022). A mutation linked to degt-1(ok3307) in C. elegans strain VC2633 affects rpm-1. microPublication Biology. 10.17912/micropub.biology.000565. PMC ID: PMC9073554.] Mutagen: Outcrossed: x4 Made by: Eugene Jennifer Jin Received: 05/12/22 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ29092 Species: C. elegans Genotype: jsIs973 III; efa-6(*ju1658[GFP::EFA-6] ju1903) IV. Description: jsIs973 [mec-7p::mRFP + unc-119(+)] III. Strong RFP cytosolic marker for the mechanosensory neurons. ju1903 deletion removes N-terminus of EFA-6 and disrupts all known isoforms. No visible GFP::EFA-6 due to ju1903 deletion. Reference: Sandhu A., et al. Cell Reports 2024 Oct 22;43(10):114776. doi: 10.1016/j.celrep.2024.114776. PMID: 39305484. Mutagen: Outcrossed: x2 Made by: Anjali Sandhu, Xiaohui Lyu, Yishi Jin Received: 02/28/25 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ29114 Species: C. elegans Genotype: bli-6(ju1914[bli-6::mNG::3xFLAG]) IV. Description: mNeonGreen tag inserted at C-terminus of endogenous bli-6 locus using Dickinson method. Superficially wild-type with green fluorescence in L4 epidermis and adult stage cuticle. Reference: Adams JRG, et al. Nat Commun. 2023 Nov 18;14(1):7506. doi: 10.1038/s41467-023-43058-9. PMID: 37980413. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jennifer Gotenstein Adams Received: 12/01/23 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ29326 Species: C. elegans Genotype: col-12::mNG(ju1932) V. Description: mNG inserted at C-terminus of endogenous col-12 locus. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jennifer Gotenstein Adams Received: 12/04/24 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ3011 Species: Caenorhabditis elegans Genotype: unc-16(ju146) III. Description: Uncoordinated and egg-laying defective. T to C transition resulting in L75P. Mutagen: EMS Outcrossed: x4 Made by: Dana Byrd Received: 02/25/02 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ3086 Species: Caenorhabditis elegans Genotype: kin-1(ok338)/unc-54(r293) I. Description: Heterozygotes are WT and segregate WT, Unc, and early larval lethal. Mutagen: UV/TMP Outcrossed: x Made by: X Huang/Y Jin Received: 10/21/02 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ3103 Species: Caenorhabditis elegans Genotype: juIs167. Description: juIs167 [vab-19::GFP + rol-6(su1006)]. Integrated by UV/TMP. Rollers. Received new stock Feb 2005. Mutagen: Outcrossed: x Made by: Mei Ding Received: 08/23/04 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ31328 Species: C. elegans Genotype: efa-6(ju1658[GFP::EFA-6]) IV. Description: Endogenous efa-6 locus tagged with GFP using CRISPR/Cas9. GFP is visible under compound fluorescence microscope. Reference: Sandhu A., et al. Cell Reports 2024 Oct 22;43(10):114776. doi: 10.1016/j.celrep.2024.114776. PMID: 39305484. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Xeufeng Meng, Xiaohui Lyu, Yishi Jin Received: 02/28/25 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ333 Species: Caenorhabditis elegans Genotype: juIs1 IV. Description: juIs1 [unc-25p::snb-1::GFP + lin-15(+)] IV. GFP expression in presynaptic terminals of GABAergic DD and VD motor neurons and RME neurons. Maintain under normal conditions. Reference: Hallan SJ and Jin Y. Nature. 1998 Sep 3;395(6697):78-82. Mutagen: UV Outcrossed: x4 Made by: Yishi jin Received: 12/01/11 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ337 Species: Caenorhabditis elegans Genotype: vab-1(dx31) II. Description: Strong Vab-1 phenotype. About 50% embryonic lethality and about 30% larval lethality. Genetic null. Mutagen: UV Outcrossed: x1 Made by: Andrew Chisholm Received: 05/06/98 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ3391 Species: Caenorhabditis elegans Genotype: vab-3(ju468) X. Description: Vab. Notched Head. Distal tip cell Mig. Male tail ray and spicule defects. Males do not mate. About 50% larval lethality. H and B cell lineage defects. Received new stock Jan. 2006. Mutagen: TMP-UV Outcrossed: x3 Made by: Nese Cinar Received: 12/28/04 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ3464 Species: Caenorhabditis elegans Genotype: juIs176. Description: juIs176 [IFB-1a::GFP + rol-6(su1006)]. Some worms don't roll. GFP expression patterns are similar to MH4 staining. Mutagen: TMP/UV Outcrossed: x Made by: Wei-Meng Woo Received: 06/28/04 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ3714 Species: Caenorhabditis elegans Genotype: gcy-31(ok296) X. Description: 2505bp deletion in cosmid T07D1. Break points are 6562 and 9069 with respect to T07D1. Sequence at the break point is: GGAAAAAAAAACTTCGCG / TTTGGCTAGTCGTAT. Primers: ok296u1: CTGAAACCATCTGACAGA; ok296d1: CATCGGAATAGGATTGTTG; ok296d2: CATTAGGTTTACAGGCTTAG. ok296d1u1 = 290bp product with WT allele. ok296d2u1 = 352 bp product with ok296 allele. Mutagen: UV/TMP Outcrossed: x6 Made by: Martin Hudson Received: 08/19/03 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CZ3715 Species: Caenorhabditis elegans Genotype: gcy-33(ok232) V. Description: 1237bp deletion in cosmid F57F5. Break points are 743 and 1980 with respect to F57F5. Sequence at the break point is: TGAGAAGTTTATAAAAAAGTA / AAACTTAAGAGTTTTCAGTCA. Primers: ok232u1: GGATTGCTTACGTGCATC; ok232d1: ATTACATTTGCAGAAACTCG; ok232d2: CTCTTCTCACTCAAATGATG. ok232u1/d1 = 322bp product with WT allele. ok232d2/u1 = 397bp product with ok232 allele. Mutagen: UV/TMP Outcrossed: x4 Made by: Martin Hudson Received: 08/19/03 Rockefeller University, New York, NY ----------------------------------------------------------------------------- Strain: CZ375 Species: Caenorhabditis elegans Genotype: vab-1(e856) II. Description: Mutagen: Outcrossed: x2 Made by: Andrew Chisholm Received: 02/26/08 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ3761 Species: Caenorhabditis elegans Genotype: ptp-3(mu256) II. Description: Low penetrance tail Vab and embryonic lethality. Mutagen: EMS Outcrossed: x2 Made by: Brian Ackley Received: 01/29/08 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ401 Species: Caenorhabditis elegans Genotype: vab-19(e1036) II. Description: Cold sensitive lethal. Vab at 20 and 25C. Mutagen: Outcrossed: x3 Made by: Andrew Chisholm Received: 08/23/04 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ4019 Species: Caenorhabditis elegans Genotype: juEx595. Description: juEx595 [IFB-1b::GFP + rol-6(su1006)]. Maintain by picking Rollers. GFP expression patterns are similar to MH4 staining. Maintain by picking rollers. Mutagen: Outcrossed: x1 Made by: Wei-Meng Woo Received: 06/28/04 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ4111 Species: Caenorhabditis elegans Genotype: vab-2(ju1) IV. Description: vab-2(ju1) has embryonic lethality (12%) and notched heads (about 40%). vab-2(ju1) is considered a null allele (W30opal). pka efn-1. Mutagen: EMS Outcrossed: x>5 Made by: Andrew Chisholm Received: 04/05/07 Queen's University, Kingston, ON, Canada ----------------------------------------------------------------------------- Strain: CZ414 Species: Caenorhabditis elegans Genotype: vab-1(e699) II. Description: Intermediate Vab-1 phenotype. About 9% embryonic lethality and about 20% larval lethality. CB699 outcrossed 2X to N2 to make CZ414. Mutagen: EMS Outcrossed: x2 Made by: Andrew Chisholm Received: 05/06/98 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ419 Species: Caenorhabditis elegans Genotype: dapk-1(ju4) I. Description: Abnormal head morphology, slightly temperature sensitive. Maintain under normal conditions. Reference: Tong A, et al. Proc Natl Acad Sci U S A. 2009 Feb 3;106(5):1457-61. Mutagen: Outcrossed: x2 Made by: Andrew Chisholm Received: 01/18/12 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ4213 Species: Caenorhabditis elegans Genotype: mkk-4(ju91) X. Description: Suppressor of rpm-1. Mutagen: EMS Outcrossed: x Made by: Xun Huang Received: 07/20/05 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ4280 Species: Caenorhabditis elegans Genotype: eps-8(jc36)/unc-26(e205) dpy-4(e1166) IV. Description: Heterozygotes segregate wild-type heterozygotes, Emb, and Unc Dpy. Maintain by picking wild-type. Mutagen: Outcrossed: x Made by: Mei Ding Received: 12/17/09 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ429 Species: Caenorhabditis elegans Genotype: vab-1(ju8) II. Description: Mutagen: Outcrossed: x2 Made by: Andrew Chisholm Received: 02/26/08 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ4380 Species: Caenorhabditis elegans Genotype: ifb-1(ju71) II. Description: Incompletely penetrant embryonic lethal at three-fold. Mild Mua. Abberant head epidermal morphology. PKA vab-21. Mutagen: EMS Outcrossed: x3 Made by: Smelser & Cerna Received: 06/28/04 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ4601 Species: C. elegans Genotype: syd-2(ju487) X. Description: ju487 is a gain-of-function allele of syd-2, changing Arg184 to Cys. Reference: Dai Y, et al. Nat Neurosci. 2006 Dec;9(12):1479-87. doi: 10.1038/nn1808. PMID: 17115037. Mutagen: EMS Outcrossed: x4 Made by: Ya Dai, Yishi Jin Received: 06/20/23 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ4733 Species: Caenorhabditis elegans Genotype: spon-1(ju430) II. Description: Temperature-sensitive. Maintain at 15C. Vab. Lethal at 25C. 2-fold Lpy. Reference: Woo WM, et al. Development. 2008 Aug;135(16):2747-56. Mutagen: Outcrossed: x3 Made by: Wei-Ming Woo Received: 03/14/12 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ4734 Species: Caenorhabditis elegans Genotype: spon-1(e2623) II. Description: Maintain under normal conditions. Weak bent head. Reference: Woo WM, et al. Development. 2008 Aug;135(16):2747-56. Mutagen: Outcrossed: x2 Made by: Wei-Ming Woo Received: 03/14/12 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ5261 Species: C. elegans Genotype: juIs198 V. Description: juIs198 [unc-25p::YFP::rab-5 + ttx-3p::RFP] V. YFP-labeled RAB-5 synaptic vesicles in GABAergic motor neurons. Reference: Sann SB, Crane MM, Lu H, Jin Y. PLoS One. 2012;7(6):e37930. Mutagen: UV/TMP Outcrossed: x0 Made by: Heather Brown Received: 12/05/17 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ540 Species: Caenorhabditis elegans Genotype: ptp-3(op147) II. Description: Low penetrance tail vab (3-5%). Low penetrance embryonic lethality (3-5%). Low penetrance larval lethality (3%). Mutagen: Tc1 Outcrossed: x10 Made by: M Gutch/M Hengartner Received: 12/26/02 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ5686 Species: C. elegans Genotype: vab-1(e2027) ptp-3(mu256)/mIn1 [mIs14 dpy-10(e128)] II. Description: mIs14 [myo-2::GFP + pes-10::GFP]. Homozygous lethal double mutant balanced by GFP- and dpy-10-marked inversion. Pick wild-type GFP+ to maintain. Heterozygotes are wild-type with pharyngeal GFP signal, and segregate wild-type GFP+, Dpy bright GFP+ (mIn1 homozygotes), and non-GFP homozygous vab-1 ptp-1 double mutants. vab-1 ptp-1 double mutants (non-GFP) are embryonic lethal. Reference: Harrington RJ, et al. Development. 2002 May;129(9):2141-53. Mutagen: Outcrossed: x0 Made by: Brian Ackley Received: 04/02/18 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ5730 Species: Caenorhabditis elegans Genotype: dlk-1(ju476) I. Description: Suppressor of rpm-1. Mutagen: EMS Outcrossed: x Made by: Katsunori Nakata Received: 08/25/05 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ5847 Species: Caenorhabditis elegans Genotype: spon-1(ju402) II; juEx1111. Description: juEx1111 [spon-1::vGFP]; rescues ju402 lethality. Vab. 2-fold Lpy. ju402 is lethal. Reference: Woo WM, et al. Development. 2008 Aug;135(16):2747-56. Mutagen: Outcrossed: x0 Made by: Emily Berry Received: 03/14/12 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ631 Species: Caenorhabditis elegans Genotype: juIs14 IV. Description: juIs14 [acr-2p::GFP + lin-15(+)] IV. GFP expression in cholinergic motor neurons. Reference: Hallam SJ, et al. Development. 2000 Oct;127(19):4239-52. Mutagen: X-ray radiation Outcrossed: x2 Made by: Yishi Jin Received: 09/01/16 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ7112 Species: Caenorhabditis elegans Genotype: lin-31(n301) vab-1(e2027) II. Description: Muv. Mutagen: Outcrossed: x Made by: Andrew Chisholm Received: 11/10/06 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ7575 Species: C. elegans Genotype: ebax-1(ju699) IV; juEx1434. Description: juEx1434 [rgef-1p::ebax-1::GFP + rol-6(su1006)]. Pick Rollers to maintain. juEx1434 rescues Egl of pqn-55(ju699) mutants. Reference: Wang Z., et al. Neuron. 2013 Sep 4;79(5):903-16. Mutagen: Outcrossed: x0 Made by: Yanli Hou Received: 12/03/20 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ793 Species: Caenorhabditis elegans Genotype: vab-1(e2027) II; lin-15B&lin-15A(n765) X; juIs24. Description: juIs24 [vab-1::GFP + lin-15(+)]. GFP very faint. Overtly WT. Mutagen: Outcrossed: x2 Made by: Inessa Grinberg Received: 04/10/07 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ8332 Species: C. elegans Genotype: juIs223 IV. Description: juIs223 [ttr-39p::mCherry + ttx-3p::GFP] IV. Transcriptional reporter with ttr-39 promoter driving mCherry expression in DD and VD neurons. Reference: Jospin M, et al. PLoS Biol. 2009 Dec;7(12):e1000265. Mutagen: Outcrossed: x0 Made by: Yingchuan Qi Received: 10/02/17 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ8920 Species: Caenorhabditis elegans Genotype: cebp-1(tm2807) X. Description: Superficially wild-type. Strong defects in axon regeneration. Maintain under normal conditions. Reference: Yan D, et al. Cell. 2009 Sep 4;138(5):1005-18. Mutagen: Outcrossed: x3 Made by: Dong Yan Received: 05/16/13 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ900 Species: Caenorhabditis elegans Genotype: syd-2(ju37) X. Description: Moderately Egl, Unc. Mutagen: EMS Outcrossed: x>1 Made by: M. Zhen & Y. Jin Received: 01/10/00 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ910 Species: Caenorhabditis elegans Genotype: vab-1(e2027) II. Description: vab-1 null phenotype. e2027 is a 74 bp deletion allele. Mutagen: Outcrossed: x4 Made by: Andrew Chisholm Received: 09/27/04 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ9236 Species: C. elegans Genotype: juIs252 V. Description: juIs252 [mec-4p::mCherry + ttx-3p::RFP] V. mCherry expression in touch neurons. Reference: Knowlton WM, et al. Front Neurosci. 2017 May 10;11:263. doi: 10.3389/fnins.2017.00263. Chuang M, et al. 2014 Cell Rep, 9, 874-83. Mutagen: Outcrossed: x3 Made by: Emily Berry Received: 02/21/18 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ9676 Species: C. elegans Genotype: acr-2(n2595 n2420) X. Description: Intragenic suppression of n2420 gain of function; n2595 is a nonsense mutation of acr-2. G to A at residue 525, causing W175-STOP. (WT: CACGGAGATGTGACATGGGTCCCACCTGCAATGTT) (n2595: CACGGAGATGTGACATGAGTCCCACCTGCAATGTT). Reference: Jospin M, et al. PLoS Biol. 2009 Dec;7(12):e1000265. Mutagen: EMS Outcrossed: x2 Made by: Tammy Stawicki Received: 05/29/18 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ9912 Species: C. elegans Genotype: ebax-1(ju699) IV. Description: Mild Unc and Egl. Reference: Wang Z., et al. Neuron. 2013 Sep 4;79(5):903-16. Mutagen: Outcrossed: x5 Made by: Zhiping Wang Received: 12/03/20 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ9957 Species: Caenorhabditis elegans Genotype: gtl-2(n2618) IV. Description: Maintain under normal conditions. Reference: Stawicki TM, et al. Curr Biol. 2011 May 24;21(10):883-8. Mutagen: Outcrossed: x0 Made by: Tammy Stawicki Received: 01/27/12 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: CZ996 Species: Caenorhabditis elegans Genotype: juIs52. Description: juIs52 [vab-2::GFP + rol-6(su1006)]. Rescuing GFP-tagged VAB-2 transgene. Rollers. vab-2 formerly known as efn-1. Mutagen: Outcrossed: x6 Made by: Ian Chin-Sang Received: 06/07/13 University of California, San Diego, CA ----------------------------------------------------------------------------- Strain: DA1006 Species: Caenorhabditis elegans Genotype: egl-19(ad1006) IV. Description: Egl. Lon. Sluggish Unc. Eat. Mutagen: EMS Outcrossed: x Made by: Raymond Lee Received: 07/17/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1013 Species: Caenorhabditis elegans Genotype: egl-19(ad1013) IV. Description: Weak Egl. Weak Eat. Mutagen: EMS Outcrossed: x2 Made by: Raymond Lee Received: 07/17/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1015 Species: Caenorhabditis elegans Genotype: egl-19(ad1015) IV. Description: Egl. Weak sluggish Unc. Mutagen: EMS Outcrossed: x3 Made by: Raymond Lee Received: 07/17/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1025 Species: Caenorhabditis elegans Genotype: vab- (ad1026); egl-19(ad1025)/bli-6(sc16) unc-24(e138) IV. Description: Impenetrant Vab - mostly tail; not mapped. Strain throws early larval lethals (ad1025 homozygotes) and Bli Uncs. Mutagen: EMS Outcrossed: x2 Made by: Raymond Lee Received: 07/17/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1031 Species: Caenorhabditis elegans Genotype: egl-19(n582) IV; unc-2(e55) X. Description: Unc. Egl, Lon, Slow and Floppy. Mutagen: Outcrossed: x Made by: Raymond Lee Received: 06/23/00 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1034 Species: Caenorhabditis elegans Genotype: egl-19(n2368) IV; unc-2(e55) X. Description: Unc. Semidominant Egl-c, Sma. Mutagen: Outcrossed: x Made by: Raymond Lee Received: 06/23/00 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1035 Species: Caenorhabditis elegans Genotype: egl-19(ad695) IV; unc-2(e55) X. Description: Unc. Semi-dominant Eat (TB relaxation defective). Mutagen: Outcrossed: x Made by: Raymond Lee Received: 06/23/00 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1042 Species: Caenorhabditis elegans Genotype: egl-19(ad1008) unc-24(e138) IV/nT1 [unc-?(n754) let-?] (IV;V) Description: Heterozygotes are Unc and segregate Uncs and dead eggs. ad1008 homozygotes are Pat. Mutagen: EMS (ad1008) Outcrossed: x Made by: Raymond Lee Received: 11/12/96 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1046 Species: Caenorhabditis elegans Genotype: hDf10 dpy-5(e61) unc-29(e403) I; sDp2 (I;f). Description: Animals carrying sDp2 are Unc and segregate Unc and dead eggs. Mutagen: Outcrossed: x Made by: MW Davis Received: 03/23/98 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1051 Species: Caenorhabditis elegans Genotype: avr-15(ad1051) V. Description: Starved. Lacks M3 spikes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS on RC301 Outcrossed: x2 Made by: Joe Dent Received: 04/06/98 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1055 Species: Caenorhabditis elegans Genotype: unc-29(e1072) eat-18(ad820) I. Description: Unc. Semi-dominant Eat. pka eat-18. Mutagen: EMS Outcrossed: x Made by: Raizen/Lee Received: 08/04/95 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1060 Species: Caenorhabditis elegans Genotype: +/eT1 III; adDf1059/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Mutagen: Gamma Rays Outcrossed: x Made by: Joe Dent Received: 05/09/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1075 Species: Caenorhabditis elegans Genotype: tln-1(e259) dpy-5(e61) I. Description: Dpy. Unc. Mutagen: Outcrossed: x Made by: Leon Avery Received: 08/04/95 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1077 Species: Caenorhabditis elegans Genotype: egl-30(ad810) dpy-5(e61)/szT1 [lon-2(e678)] I; +/szT1 X. Description: ad810 is homozygous lethal. ad810/+ is Egl and it suppresses gpb-2 (a.k.a. eat-11). In DA1077, heterozygotes are Egl. Strain throws Lon Males. Mutagen: Outcrossed: x Made by: Leon Avery Received: 06/28/99 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1084 Species: Caenorhabditis elegans Genotype: egl-30(ad806) I. Description: Egl. Semi-dominant suppressor of eat-11. Mutagen: EMS Outcrossed: x Made by: Leon Avery Received: 05/13/02 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1096 Species: Caenorhabditis elegans Genotype: egl-30(ad810)/szT1 [lon-2(e678)] I; +/szT1 X. Description: ad810 is homozygous lethal. ad810/+ is Egl and it suppresses gpb-2 (a.k.a. eat-11). In DA1096, heterozygotes are Egl. Throws Lon males. Mutagen: Outcrossed: x Made by: Leon Avery Received: 06/28/99 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1110 Species: Caenorhabditis elegans Genotype: eat-18(ad1110) I. Description: Eat. Slow pumping. pka eat-18. Mutagen: EMS Outcrossed: x2 Made by: David Raizen Received: 07/17/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1113 Species: Caenorhabditis elegans Genotype: eat-2(ad1113) II. Description: Eat. Slow pumping. Mutagen: EMS Outcrossed: x2 Made by: David Raizen Received: 07/17/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1116 Species: Caenorhabditis elegans Genotype: eat-2(ad1116) II. Description: Eat. Slow pumping. Long lived. Embryonic lethality observed in a significant fraction of animals is likely explained by a mutation in an essential gene linked to eat-2 Mutagen: EMS Outcrossed: x2 Made by: David Raizen Received: 07/17/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1204 Species: Caenorhabditis elegans Genotype: adEx1204. Description: adEx1204 [rol-6(d) + eat-4::lacZ]. Medium transmission. Mutagen: Outcrossed: x Made by: Raymond Lee Received: 08/27/99 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1240 Species: Caenorhabditis elegans Genotype: adIs1240 lin-15B&lin-15A(n765) X. Description: adIs1240 [eat-4::sGFP + lin-15(+)] X. Mutagen: Gamma Rays Outcrossed: x Made by: Raymond Lee Received: 01/12/98 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1241 Species: Caenorhabditis elegans Genotype: eat-4(ky5) III; lin-15B&lin-15A(n765) X; adEx1241. Description: adEx1241 [eat-4(+) + lin-15(+)]. Animals with the array are WT. Animals which have lost the array are Muv and Eat. n765 is temperature-sensitive. Mutagen: Outcrossed: x Made by: Raymond Lee Received: 04/06/98 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1242 Species: Caenorhabditis elegans Genotype: eat-4(ky5) III; lin-15B&lin-15A(n765) X; adEx1242. Description: adEx1242 [eat-4(+) + lin-15(+)]. Animals with the array are WT. Animals which have lost the array are Muv and Eat. n765 is temperature sensitive. Mutagen: Outcrossed: x Made by: Raymond Lee Received: 08/11/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1243 Species: Caenorhabditis elegans Genotype: eat-4(ky5) III; adIs1240 lin-15B&lin-15A(n765) X. Description: adIs1240 [eat-4::sGFP + lin-15(+)] X. Mutagen: Outcrossed: x Made by: Raymond Lee Received: 08/11/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1256 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; adEx1256. Description: adEx1256 [egl-19::sGFP-NLS + lin-15(+)]. Animals with the array are WT. Animals which have lost the array are Muv. n765 is temperature-sensitive. Mutagen: Outcrossed: x Made by: Raymond Lee Received: 01/10/00 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1262 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; adEx1262. Description: adEx1262 [gcy-5::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive. Mutagen: Outcrossed: x Made by: S. Yu Received: 11/13/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1266 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; adEx1266. Description: adEx1266 [gcy-12::GFP + lin-15(+)]. Maintain by picking non-Muv. Very weak GFP signal. n765 is temperature-sensitive. Mutagen: Outcrossed: x Made by: S. Yu Received: 11/26/01 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1267 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; adEx1267. Description: adEx1267 [gcy-8::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive. Mutagen: Outcrossed: x Made by: S. Yu Received: 11/20/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1269 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; adEx1269. Description: adEx1269 [odr-1::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive. Mutagen: Outcrossed: x Made by: S. Yu Received: 11/20/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1276 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; adEx1276. Description: adEx1276 [gcy-?::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive. Mutagen: Outcrossed: x Made by: S. Yu Received: 11/20/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1288 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; adEx1288. Description: adEx1288 [gcy-7::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive. Mutagen: Outcrossed: x Made by: S. Yu Received: 11/20/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1290 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; adEx1290. Description: adEx1290 [gcy-33::GFP + lin-15(+)]. Maintain by picking non-Muv. gcy-33::GFP in BAG. n765 is temperature-sensitive. Mutagen: Outcrossed: x Made by: S. Yu Received: 10/13/99 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1292 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; adEx1292. Description: adEx1292 [R01E6.1_8k::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive. Mutagen: Outcrossed: x Made by: S. Yu Received: 11/13/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1295 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; adEx1295. Description: adEx1295 [gcy-32::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive. Mutagen: Outcrossed: x Made by: S. Yu Received: 11/26/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1296 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; adEx1296. Description: adEx1296 [gcy-32::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive. Mutagen: Outcrossed: x Made by: S. Yu Received: 11/26/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1297 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; adEx1297. Description: adEx1297 [gcy-6::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive. Mutagen: Outcrossed: x Made by: S. Yu Received: 11/13/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1299 Species: Caenorhabditis elegans Genotype: adEx1299. Description: adEx1299 [avr-15::GFP + rol-6(su1006)]. GFP expression in pharyngeal muscle and some extrapharyngeal neurons. Maintain by picking Rollers. Mutagen: Microinjection Outcrossed: x0 Made by: Joe Dent Received: 10/13/05 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1302 Species: Caenorhabditis elegans Genotype: avr-14(ad1305) I; avr-15(ad1051) V. Description: Moderate resistance to ivermectin. NOTE: originally described as carrying ad1302, but reported to actually be ad1305; see strain JD740 for verified avr-14(ad1302) I; avr-15(ad1051) V double mutant. Do not distribute this strain; other labs should request it from the CGC. Mutagen: EMS Outcrossed: x2 Made by: Joe Dent Received: 08/29/00 McGill University, Montreal QC, Canada ----------------------------------------------------------------------------- Strain: DA1316 Species: Caenorhabditis elegans Genotype: avr-14(ad1305) I; avr-15(vu227) glc-1(pk54) V. Description: Highly resistant to ivermectin. This strain cannot be sent to commercial recipients without approval from UT Southwestern. Do not distribute this strain; other labs should request it from the CGC. Received new stock 5/21/08. [NOTE: The correct genotype of this strain is avr-14(ad1305) I; avr-15(vu227) glc-1(pk54) V. This strain was incorrectly annotated as avr-14(ad1302) I; avr-15(ad1051) glc-1(pk54) V. when submitted to the CGC.] Mutagen: Tc1 Outcrossed: x Made by: Received: 08/29/00 McGill University, Montreal QC, Canada ----------------------------------------------------------------------------- Strain: DA1350 Species: Caenorhabditis elegans Genotype: adEx1350. Description: adEx1350 [kin-18::GFP + rol-6(su1006)]. Pick Rollers. Only about 10% transmission. Mutagen: Outcrossed: x Made by: M Hutchison Received: 07/12/02 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1370 Species: Caenorhabditis elegans Genotype: avr-15(vu227) glc-1(pk54) V. Description: Lacks M3 spikes. glc-1(pk54::Tc1). This strain cannot be sent to commercial recipients without approval from UT Southwestern. Do not distribute this strain; other labs should request it from the CGC. Received new stock 5/21/08. [NOTE: The correct genotype of this strain is avr-15(vu227) glc-1(pk54) V. This strain was incorrectly annotated as avr-15(ad1051) glc-1(pk54) V. when submitted to the CGC.] Mutagen: Outcrossed: x Made by: Joe Dent Received: 12/20/05 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1371 Species: Caenorhabditis elegans Genotype: avr-14(ad1302) I. Description: WT. This strain cannot be sent to commercial recipients without approval from UT Southwestern. Do not distribute this strain; other labs should request it from the CGC. Mutagen: EMS Outcrossed: x2 Made by: Joe Dent Received: 08/29/00 McGill University, Montreal QC, Canada ----------------------------------------------------------------------------- Strain: DA1384 Species: Caenorhabditis elegans Genotype: avr-14(ad1302) I; glc-1(pk54) V. Description: glc-1(pk54::Tc1). High level of ivermectin resistance. This strain cannot be sent to commercial recipients without approval from UT Southwestern. Do not distribute this strain; other labs should request it from the CGC. Mutagen: Outcrossed: x Made by: Joe Dent Received: 12/20/05 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1402 Species: Caenorhabditis elegans Genotype: eat-5(ad1402) I. Description: Small intragenic deletion. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. Mutagen: EMS Outcrossed: x5 Made by: Leon Avery Received: 01/15/03 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1426 Species: Caenorhabditis elegans Genotype: exp-2(sa26ad1426) V. Description: Reversion of sa26. Severely reduced R spikes, long pumps, short hold-backs. Mutagen: ENU Outcrossed: x2 Made by: M. Wayne Davis Received: 04/06/98 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1519 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; adEx1519. Description: adEx1519 [M03F4.3::GFP + lin-15(+)]. Putative serotonin receptor. Maintain by picking non-Muv. n765 is temperature-sensitive. Mutagen: Outcrossed: x Made by: Niacaris & Avery Received: 07/24/03 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DA1616 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; adEx1616. Description: adEx1616 [ser-4::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive. Mutagen: Outcrossed: x Made by: Tim Niacaris Received: 07/24/03 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DA1641 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; adEx1641. Description: adEx1641 [C09B7.1::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive. Mutagen: Outcrossed: x Made by: Tim Niacaris Received: 07/24/03 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DA1644 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; adEx1644. Description: adEx1644 [K02F2.6::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive. Mutagen: Outcrossed: x Made by: Tim Niacaris Received: 07/24/03 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DA1646 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; adEx1646. Description: adEx1646 [T02E9.3::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive. Mutagen: Outcrossed: x Made by: Tim Niacaris Received: 07/24/03 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DA1647 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; adEx1647. Description: adEx1647 [dop-1(trans)::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive. Mutagen: Outcrossed: x Made by: Received: 07/24/03 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DA1648 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; adEx1648. Description: adEx1648 [F01E11.5::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive. Mutagen: Outcrossed: x Made by: Tim Niacaris Received: 07/24/03 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DA1649 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; adEx1649. Description: adEx1649 [F14D12.6::GFP + lin-15(+)]. Maintain by picking non-Muv. n765 is temperature-sensitive. Mutagen: Outcrossed: x Made by: Tim Niacaris Received: 07/24/03 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DA1674 Species: Caenorhabditis elegans Genotype: acr-19(ad1674) I. Description: Deletion of bp 1108-3194 of C31H5.3 Mutagen: UV/TMP Outcrossed: x1 Made by: T Niacaris Received: 10/28/02 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1750 Species: Caenorhabditis elegans Genotype: adEx1750. Description: adEx1750 [pmk-3::GFP + rol-6(su1006)]. Pick Rollers to maintain. Nuclear GFP in anterior and posterior intestine. [NOTE: adEx1750 contains a F42G8.4::GFP reporter construct. This array had been previously described as carrying a pmk-1::GFP reporter; the description of the array was updated in CGC records ~2016. islo-1, pmk-3, pmk-2, and pmk-1 are in an operon. The order of genes was described in Berman et al. as [pmk-1(F42G8.4)->pmk-2(F42G8.3)->pmk-3(B0218.3)], but the official WormBase gene names were assigned in reverse order [pmk-1(B0218.3)->pmk-2(F42G8.3)->pmk-3(F42G8.4)]. See Berman K, et al. Mol Cell Biol Res Commun. 2001 Nov;4(6):337-44. PMID: 11703092 for additional information.] Mutagen: Outcrossed: x Made by: K. Berman Received: 01/26/06 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1774 Species: Caenorhabditis elegans Genotype: ser-3(ad1774) I. Description: Deletion of bp 433-1994 of K02F2.6. Mutagen: UV/TMP Outcrossed: x3 Made by: T. Niacaris Received: 07/26/01 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1814 Species: Caenorhabditis elegans Genotype: ser-1(ok345) X. Description: F59C12.2. Homozygous. Outer Left Sequence: AAGCATCTTTGAGCGCATTT. Outer Right Sequence: CATAGCGAGTGTTTGGAGCA. Inner Left Sequence: AATTTCAGGGGTGTGGACAT. Inner Right Sequence: AATCATTTTTGAAACCGACCC. Inner Primer PCR Length: 2926 bp. Deletion Size: 859 bp. Deletion left flank: TGTTTTGTAAGCTTTGTAAAATTATGTAGT. Deletion right flank: CCACTAGAAATAATTTCCCCCTTCTTTTTC. URL: http://www.celeganskoconsortium.omrf.org. Mutagen: Outcrossed: x10 Made by: L. Avery Received: 03/01/02 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1877 Species: Comamonas sp. Genotype: Comamonas sp. Description: Bacteria. Comamonas sp., a bacterium on which C. elegans grows particularly well. Str-R. DA1877 is derived from a bacterium isolated from soil in the Dallas area by Boris Shtonda in 2002. That strain was called H39 in Avery, L, Shtonda, BB (2003), "Food transport in the C elegans pharynx", J Exp Biol 206: 2441-2457. It was identified as genus Comamonas by 16S rDNA sequencing, as described in the paper. L. Avery isolated a spontaneous streptomycin-resistant variant by selecting for growth in LB broth + 200 ug/ml streptomycin sulfate. This strain, when spread on NGMSR plates, gave rise to faster-growing papillae; one of these was streaked out to get DA1877. Biosafety Level: BSL-1. Mutagen: Outcrossed: x Made by: Leon Avery Received: 09/09/04 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1880 Species: Bacillus megaterium Genotype: Bacillus megaterium. Description: Bacteria. Str-R. L10 papilla 2; sporulation-defective mutant. This is a low-quality food that is difficult for the worms to eat, and is useful for studies of the effect of food on behavior, physiology, etc. [NOTE: This strain grows better on NGM than on LB media in CGC.] Described in J Exp Biol 206: 2441-2457. Biosafety Level: BSL-1. Mutagen: Outcrossed: x Made by: Received: 01/27/05 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1885 Species: Bacillus simplex Genotype: B. simplex Description: Bacteria. Str-R. Streak and maintain on Str+ plates. NGMSR+. Faster growing papilla on NGMSR. [NOTE: This strain grows better on NGM than on LB media in CGC.] Biosafety Level: BSL-1. Mutagen: Outcrossed: x Made by: B Shtonda/L Avery Received: 12/04/09 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA1917 Species: Escherichia coli Genotype: E. coli. Description: Bacteria. Contains eat-5 rescuing plasmid pRE5-7. Amp-R. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. Biosafety Level: BSL-1. Mutagen: Outcrossed: x Made by: Leon Avery Received: 01/15/03 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA2100 Species: Caenorhabditis elegans Genotype: ser-7(tm1325) X. Description: Lack of 5HT stimulation of pumping. Primers GGCCTGCCTTCCTGACATGT, CGCGGATTCTCTATCAATAG, ATCCTG GAGCTGGCGAGTTA, GACTGTAAACGCGCAGAGTC. Mutation site 42634-42635 - GGGAANNAAAACCCTCCCTNNANNANNATNNGCANNCC - 43376-43377. 742 bp deletion + 38 bp insertion. Mutagen: UV/TMP Outcrossed: x10 Made by: Leon Avery Received: 04/27/06 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA2109 Species: Caenorhabditis elegans Genotype: ser-7(tm1325) ser-1(ok345) X. Description: F59C12.2. Homozygous. Outer Left Sequence: AAGCATCTTTGAGCGCATTT. Outer Right Sequence: CATAGCGAGTGTTTGGAGCA. Inner Left Sequence: AATTTCAGGGGTGTGGACAT. Inner Right Sequence: AATCATTTTTGAAACCGACCC. Inner Primer PCR Length: 2926 bp. Deletion Size: 859 bp. Deletion left flank: TGTTTTGTAAGCTTTGTAAAATTATGTAGT. Deletion right flank: CCACTAGAAATAATTTCCCCCTTCTTTTTC. URL: http://www.celeganskoconsortium.omrf.org. Mutagen: Outcrossed: x Made by: Leon Avery Received: 04/27/06 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA2123 Species: Caenorhabditis elegans Genotype: adIs2122. Description: adIs2122 [lgg-1p::GFP::lgg-1 + rol-6(su1006)]. Rollers. Mutagen: Gamma Rays Outcrossed: x7 Made by: Chanhee Kang Received: 06/09/08 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA2124 Species: Escherichia coli Genotype: E. coli. Description: Bacteria. E. coli strain carrying an avr-15::GFP fusion plasmid. Amp-R. Sac I Pst I fragment from K10B8 fused to transmembrane GFP from TM-1. Biosafety Level: BSL-1. Mutagen: Outcrossed: x Made by: Joe Dent Received: 10/12/05 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA2143 Species: Caenorhabditis elegans Genotype: egl-4(ks62) IV; adEx2143. Description: adEx2143 [tax-4p::pkg-1 + rol-6p::GFP]. Maintain by picking GFP+. pkg-1 is the new name of egl-4. Reference: You et al (2008) Cell Metab 7(3):249-57. Mutagen: Outcrossed: x Made by: Youngjai You Received: 05/03/10 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA2149 Species: Caenorhabditis elegans Genotype: egl-4(ks62) IV; adEx2149. Description: adEx2149 [odr-3p::pkg-1 + rol-6p::GFP]. Maintain by picking GFP+. Reference: You et al (2008) Cell Metab 7(3):249-57. Mutagen: Outcrossed: x Made by: Youngjai You Received: 05/03/10 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA2154 Species: Caenorhabditis elegans Genotype: phb-2(ad2154) II. Description: Hemiasterlin resistant. Mutagen: EMS Outcrossed: x3 Made by: Iryna Zubovych Received: 04/10/07 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA2155 Species: Caenorhabditis elegans Genotype: har-1(ad2155) III. Description: Hemiasterlin resistant. Maintain under normal conditions. Reference: Zubovych IO, et al. Mol Biol Cell. 2010 Mar 15;21(6):956-69. Mutagen: EMS Outcrossed: x3 Made by: Iryna Zubovych Received: 01/24/12 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA2202 Species: Caenorhabditis elegans Genotype: daf-7(e1372) III; adEx2202. Description: adEx2202 [gpa-4p::daf-7 + rol-6p::GFP]. Rescues Daf-c. Maintain by picking GFP+. Reference: You et al (2008) Cell Metab 7(3):249-57. Mutagen: Outcrossed: x Made by: Youngjai You Received: 05/03/10 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA2211 Species: Escherichia coli Genotype: E. coli. Description: Bacteria. E18 eat-4 promoter/GFP translational fusion, fused Klenowed ScaI/PstI fragments of pRE4-4-YK-Sac_Pst (~4.4kb) with TU#62(~2.2kb), checked loss of PstI site. PKA pRE4-GFP. Biosafety Level: BSL-1. Mutagen: Outcrossed: x Made by: Raymond Lee Received: 10/04/07 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA2249 Species: Caenorhabditis elegans Genotype: spg-7(ad2249) I. Description: Hemiasterlin resistant. Maintain under normal conditions. Reference: Zubovych IO, et al. Mol Biol Cell. 2010 Mar 15;21(6):956-69. Mutagen: EMS Outcrossed: x3 Made by: Iryna Zubovych Received: 01/24/12 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA2250 Species: Caenorhabditis elegans Genotype: mgl-2(tm355) I; mgl-1(tm1811) X. Description: Superficially wild-type; multiple subtle phenotypes related to nutritional response. References: Kang C, You YJ, Avery L. Genes Dev. 2007 Sep 1;21(17):2161-71. Kang C, Avery L. Genes Dev. 2009 Jan 1;23(1):12-7. Mutagen: Outcrossed: x Made by: Chanhee Kang Received: 02/10/14 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA2356 Species: Caenorhabditis elegans Genotype: ced-1(e1735) I; ced-3(n717) IV; lin-15B&lin-15A(n765) X; adEx2342. Description: adEx2342 [efl-3::mCherry::FLAG + LIN-15(+)]. Maintain by picking non-Muv. Fluorescence is dim, but array is stable (>95% transmission). Mutagen: Outcrossed: x Made by: Jennifer Winn Received: 06/13/11 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA438 Species: Caenorhabditis elegans Genotype: bli-4(e937) I; rol-6(e187) II; daf-2(e1368) vab-7(e1562) III; unc-31(e928) IV; dpy-11(e224) V; lon-2(e678) X. Description: Linkage mapping strain. Maintain at 15C. Mutagen: Outcrossed: x Made by: Avery L Received: 09/08/88 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA443 Species: Caenorhabditis elegans Genotype: rol-3(e754) unc-42(e270) V. Description: Roller Unc. e754 is cold-sensitive: lethal at 15C. Mutagen: Outcrossed: x Made by: Leon Avery Received: 08/16/93 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA449 Species: Caenorhabditis elegans Genotype: eat-1(e2343) dpy-20(e1282) IV. Description: Thin, starved, healthy adults. Retain few eggs. Slow irregular pumping at 16, 20, 25C. Dpy. Mutagen: EMS Outcrossed: x Made by: Leon Avery Received: 09/20/93 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA453 Species: Caenorhabditis elegans Genotype: eat-2(ad453) II. Description: Eat. Scrawny. Slow pumping. Mutagen: EMS Outcrossed: x1 Made by: Leon Avery Received: 07/17/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA464 Species: Caenorhabditis elegans Genotype: eat-5(ad464) I. Description: Abnormal feeding. Corpus and terminal bulb contractions unsynchronized. Mutagen: EMS Outcrossed: x Made by: Avery L Received: 10/29/91 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA465 Species: Caenorhabditis elegans Genotype: eat-2(ad465) II. Description: Abnormal feeding. Slow, regular pumping. See also WBPaper00002341. Mutagen: EMS Outcrossed: x Made by: Avery L Received: 10/29/91 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA467 Species: Caenorhabditis elegans Genotype: eat-6(ad467) V. Description: Abnormal feeding. Strong relaxation defective. NOTE: This strain is reportedly homozygous for an unknown X-linked unc mutation (10/31/2017). Mutagen: EMS Outcrossed: x Made by: Avery L Received: 10/29/91 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA468 Species: Caenorhabditis elegans Genotype: unc-31(e928) IV; act-2(ad468) act-3(ad767) V. Description: Patchy birefringence in pharyngeal muscles-semidominant. Mutagen: EMS Outcrossed: x Made by: Avery L Received: 11/14/91 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA469 Species: Caenorhabditis elegans Genotype: dpy-20(e1282) unc-31(e928) IV. Description: Dpy (ts). Unc. Mutagen: Outcrossed: x Made by: Leon Avery Received: 02/22/94 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA472 Species: Caenorhabditis elegans Genotype: pha-2(ad472) X. Description: Misshapen pharynx; worms hatch with pharynx of correct gross shape, but disorganized, with nuclei misplaced. Most homozygotes arrest in L1, escapers grow up to become very starved adults with deformed pharynges with abnormally small terminal bulb, thick nucleated isthmus. Weakly cold sensitive. Makes dauers that don't recover; doesn't survive freezing well. Mutagen: EMS Outcrossed: x Made by: Avery L Received: 01/15/92 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA491 Species: Caenorhabditis elegans Genotype: dpy-20(e1282) unc-30(e191) IV. Description: Unc. Temperature sensitive Dpy. Mutagen: Outcrossed: x Made by: Received: 07/17/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA493 Species: Caenorhabditis elegans Genotype: phm-3(ad493) III. Description: Weak pharyngeal birefringence. Mutagen: X-ray Outcrossed: x Made by: Avery L Received: 10/29/91 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA496 Species: Caenorhabditis elegans Genotype: sDf10 unc-31(e169)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, Unc lethals (early larval) and dead eggs. Maintain by picking WT. Mutagen: Formaldehyde Outcrossed: x Made by: Avery L Received: 10/19/88 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA497 Species: Caenorhabditis elegans Genotype: let-59(s49) unc-22(s7)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul and lethal twitchers. Lethal early larval. Pick twitchers in 1% nicotine. Mutagen: EMS Outcrossed: x Made by: Avery L Received: 09/08/88 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA501 Species: Caenorhabditis elegans Genotype: unc-101(m1) unc-59(e261) I. Description: Mutagen: Outcrossed: x Made by: Leon Avery Received: 11/21/94 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA502 Species: Caenorhabditis elegans Genotype: unc-75(e950) unc-101(m1) I. Description: Unc. Eat. Mutagen: Outcrossed: x Made by: Leon Avery Received: 09/16/96 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA508 Species: Caenorhabditis elegans Genotype: npr-1(n1353) X. Description: Clumps. npr-1 pka bor-1. Mutagen: EMS Outcrossed: x2 Made by: Leon Avery Received: 09/06/96 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA509 Species: Caenorhabditis elegans Genotype: unc-31(e928) IV. Description: Extensively backcrossed to N2. Background strain for most of the feeding-defective mutant screens. Mutagen: Outcrossed: x10 Made by: Leon Avery Received: 09/22/93 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA521 Species: Caenorhabditis elegans Genotype: egl-4(ad450) IV. Description: Abnormal feeding. Falls asleep. Does not feed while asleep. Semidominant. Previously called eat-7. Mutagen: EMS Outcrossed: x Made by: Avery L Received: 10/29/91 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA522 Species: Caenorhabditis elegans Genotype: eat-13(ad522) X. Description: Eat mutant. Slippery pharynx. Slight relaxation defect. Mutagen: X-rays Outcrossed: x2 Made by: Leon Avery Received: 12/03/93 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA531 Species: Caenorhabditis elegans Genotype: eat-1(ad427) IV. Description: Abnormal feeding. Slow pumping. Mutagen: EMS Outcrossed: x Made by: Avery L Received: 11/07/91 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA538 Species: Caenorhabditis elegans Genotype: adDf538 I. Description: Grinder cannot come to full forward position. Protruding spicules in males; males don't mate. PKA phm-2(ad538). Mutagen: X-ray Outcrossed: x Made by: Avery L Received: 10/29/91 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA541 Species: Caenorhabditis elegans Genotype: gpb-2(ad541) I. Description: Abnormal feeding. Slippery Corpus. Long. Slight coiler Unc. gpb-2(ad541) previously called eat-11(ad541). Mutagen: X-ray Outcrossed: x Made by: Avery L Received: 10/29/91 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA563 Species: Caenorhabditis elegans Genotype: eat-9(e2337) I; him-8(e1489) IV. Description: Abnormal feeding. Irregular pumping. Throws males. Mutagen: EMS Outcrossed: x Made by: Avery L Received: 10/29/91 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA572 Species: Caenorhabditis elegans Genotype: eat-4(ad572) III. Description: Starved appearance. Reverts spontaneously-Do not passage! Clone starved-looking progeny with abnormal fast pharyngeal pumping to maintain. Mutagen: X-ray Outcrossed: x Made by: Avery L Received: 10/21/91 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA573 Species: Caenorhabditis elegans Genotype: eat-14(ad573) X. Description: Mutagen: X-ray Outcrossed: x2 Made by: Leon Avery Received: 11/29/93 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA589 Species: Caenorhabditis elegans Genotype: unc-32(e189) emb-9(hc70) III. Description: Unc. Temperature sensitive. Maintain at 15C. hc70 is semi-dominant. Mutagen: Outcrossed: x Made by: Leon Avery Received: 03/11/94 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA591 Species: Caenorhabditis elegans Genotype: unc-10(ad591) X. Description: Mild Unc-tends to form abnormally deep bends, especially when backing. Mild Eat. Mutagen: X Rays Outcrossed: x2 Made by: Leon Avery Received: 05/23/96 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA596 Species: Caenorhabditis elegans Genotype: snt-1(ad596) II. Description: Eat mutant. Strong Unc Eat. Jerky backward Unc. Hypomorph. Mutagen: X-rays Outcrossed: x2 Made by: Leon Avery Received: 12/03/93 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA597 Species: Caenorhabditis elegans Genotype: phm-2(ad597) I. Description: Mutagen: X-rays Outcrossed: x2 Made by: Leon Avery Received: 04/06/98 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA599 Species: Caenorhabditis elegans Genotype: eat-8(ad599) III. Description: Abnormal feeding. Brief, rare pumps. Slight coiler Unc. Mutagen: X-ray Outcrossed: x Made by: Avery L Received: 10/29/91 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA601 Species: Caenorhabditis elegans Genotype: eat-6(ad601) V. Description: Eat. Long pumps; slippery isthmus and corpus. Mutagen: X Rays Outcrossed: x2 Made by: Leon Avery Received: 05/23/96 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA602 Species: Caenorhabditis elegans Genotype: eat-15(ad602) I. Description: Eat mutant. Slippery isthmus, corpus and grinder. Mutagen: X-rays Outcrossed: x2 Made by: Leon Avery Received: 12/03/93 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA606 Species: Caenorhabditis elegans Genotype: eat-10(ad606) IV. Description: Abnormal feeding. Slippery isthmus and corpus. Mutagen: X-ray Outcrossed: x Made by: Avery L Received: 10/29/91 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA607 Species: Caenorhabditis elegans Genotype: pha-3(ad607) IV. Description: Deformed pharynx. Mutagen: X-ray Outcrossed: x Made by: Avery L Received: 10/29/91 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA609 Species: Caenorhabditis elegans Genotype: npr-1(ad609) X. Description: Causes worms to aggregate (Bor). Social. Mutagen: EMS Outcrossed: x2 Made by: S. Hekimi Received: 02/15/05 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA612 Species: Caenorhabditis elegans Genotype: bli-4(e937) adDf538 I. Description: Blistered. Grinder cannot come to full forward position. Protruding spicules in males; males don't mate. adDf538 previously called phm-2(ad538). Mutagen: Outcrossed: x Made by: Leon Avery Received: 09/16/96 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA620 Species: Caenorhabditis elegans Genotype: eat-2(ad465) lin-7(e1413) unc-52(e444) II. Description: Abnormal feeding: slow, regular pumping. Vulvaless (incomplete penetrance). Unc. Mutagen: Outcrossed: x Made by: Leon Avery Received: 03/03/95 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA631 Species: Caenorhabditis elegans Genotype: eat-3(ad426) II; him-8(e1489) IV. Description: Abnormal feeding. Slow, irregular feeding. eat-3 exhibits strong maternal effect; dauers seldom recover; Him. Mutagen: EMS Outcrossed: x Made by: Avery L Received: 11/07/91 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA650 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Clumps. Found in strain RC301. See 1987 Worm Meeting Abstract Book page 162. npr-1 pka bor-1. Mutagen: Outcrossed: x Made by: Received: 09/06/96 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA658 Species: Caenorhabditis elegans Genotype: lon-2(e678) npr-1(n1353) X. Description: Long. Clumps. npr-1 pka bor-1. Mutagen: Outcrossed: x Made by: Davis/Faulkner Received: 09/06/96 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA664 Species: Caenorhabditis elegans Genotype: egl-4(ad450) lin-1(e1777) IV. Description: Muv. ad450 worms, when undisturbed, fall asleep. While asleep they do not move or pump. Disturbing them wakes them up, and while awake they act fairly normal. ad450 previously called eat-7. Mutagen: EMS Outcrossed: x Made by: Leon Avery Received: 09/20/93 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA675 Species: Caenorhabditis elegans Genotype: unc-45(r450) dpy-1(e1) phm-3(ad493) III. Description: Dpy. Unc (ts). Phm. Weak pharyngeal birefringence. Mutagen: Outcrossed: x Made by: Leon Avery Received: 02/18/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA678 Species: Caenorhabditis elegans Genotype: unc-57(ad592) dpy-5(e61) I. Description: DpyUnc. ad592 is Eat. Dpy is semi-dominant. Mutagen: X-ray Outcrossed: x Made by: Leon Avery Received: 08/16/93 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA686 Species: Caenorhabditis elegans Genotype: nDf16/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. dpy-19(e1259) is temperature sensitive. Maintain by picking WT. [Checked 11/94 with sma-3 and nDf16 is present.] Mutagen: Gamma rays (nDf16) Outcrossed: x Made by: Leon Avery Received: 05/23/94 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA695 Species: Caenorhabditis elegans Genotype: egl-19(ad695) IV. Description: Abnormal feeding. Relaxation defective. Smallish. Males don't mate. Semidominant. Previously called eat-12. See also WBPaper00002912. Mutagen: EMS Outcrossed: x Made by: Avery L Received: 10/29/91 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA698 Species: Caenorhabditis elegans Genotype: unc-36(ad698) III. Description: Unc. Eat - slippery pharynx, slow pumping. Mutagen: EMS - RC301 background Outcrossed: x2 Made by: Leon Avery Received: 04/17/98 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA702 Species: Caenorhabditis elegans Genotype: eat-16(ad702) I. Description: Note: ad702 was isolated in an RC301 background. DA702 was not tested for the presence of npr-1(g320) following three rounds of outcrossing to N2 Bristol. DA702 does not display clumping behavior, so it's likely that DA702 has the Bristol npr-1. New stock rec'd 10/13/99. Mutagen: EMS Outcrossed: x3 Made by: Leon Avery Received: 11/29/93 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA707 Species: Caenorhabditis elegans Genotype: eat-17(ad707) X. Description: Eat mutant. Stuffs corpus and isthmus. Abnormality in m6 and m7 contraction timing. Displays clumping behavior; isolated in an RC301 background, so it's likely to have the RC301 bor-1 mutation. Mutagen: EMS Outcrossed: x2 Made by: Leon Avery Received: 12/03/93 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA709 Species: Caenorhabditis elegans Genotype: +/nT1 IV; sqt-3(sc63) eat-6(ad467) unc-76(e911)/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul and Sqt Unc larvae that grow very slowly. Mutagen: Outcrossed: x Made by: Leon Avery Received: 11/20/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA711 Species: Caenorhabditis elegans Genotype: unc-10(e102) dpy-6(e14) X. Description: DpyUnc. Mutagen: Outcrossed: x Made by: Leon Avery Received: 11/20/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA726 Species: Caenorhabditis elegans Genotype: unc-10(e102) eat-13(ad522) X. Description: Unc. Eat mutant. Slippery pharynx-Slight relaxation defect. Mutagen: Outcrossed: x Made by: Leon Avery Received: 03/17/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA729 Species: Caenorhabditis elegans Genotype: unc-101(m1) eat-9(e2337) I. Description: Unc. Abnormal feeding. Irregular pumping. Mutagen: Outcrossed: x Made by: Leon Avery Received: 08/04/95 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA734 Species: Caenorhabditis elegans Genotype: adDf538 unc-101(m1) I. Description: Grinder cannot come to full forward position. Protruding spicules in males; males don't mate. Unc. adDf538 previously called phm-2(ad538). Mutagen: Outcrossed: x Made by: Leon Avery Received: 09/06/96 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA735 Species: Escherichia coli Genotype: E. coli. Description: Bacteria. 15kb lin-15 Xba fragment in Bluescript. Rescues @ 25 ng/ml and 50 ng/ml, better at 25. Biosafety Level: BSL-1. Mutagen: Outcrossed: x Made by: Linda Huang Received: 10/04/07 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA740 Species: Caenorhabditis elegans Genotype: bli-6(sc16) unc-24(e138) IV. Description: Blistered Unc. Mutagen: Outcrossed: x Made by: Raymond Lee Received: 02/28/94 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA742 Species: Caenorhabditis elegans Genotype: lon-2(e678) unc-6(e78) egl-15(n484) X. Description: Long. Uncoordinated. Egl. Mutagen: Outcrossed: x Made by: M.W. Davis Received: 09/26/94 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA768 Species: Caenorhabditis elegans Genotype: bli-6(sc16) egl-19(ad695) unc-24(e138)/nDf41 IV. Description: Heterozygotes are Blistered and Eat (terminal bulb relaxation defective). Heterozygotes segregate BliEat, BliEatUnc and dead eggs. ad695 previously called eat-12. Mutagen: Outcrossed: x Made by: Raymond Lee Received: 04/20/94 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA778 Species: Caenorhabditis elegans Genotype: dpy-10(e128) snt-1(ad596) II. Description: Mutagen: Outcrossed: x Made by: Leon Avery Received: 11/29/93 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA792 Species: Caenorhabditis elegans Genotype: eat-6(ad792) V. Description: Eat. Relaxation defective. Lib. Cold sensitive. Mutagen: EMS Outcrossed: x2 Made by: Leon Avery Received: 05/23/96 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA810 Species: Caenorhabditis elegans Genotype: egl-30(ad810) gpb-2(ad541)/gpb-2(ad541) I. Description: ad810 is homozygous lethal. ad810/+ is Egl and it suppresses gpb-2. gpb-2 phenotype is rather subtle: they are slightly starved, slightly longer than normal, and tend to be loopy in their movements (they make abnormally deep bends). Hets should be Egl and non-Eat. On most E. coli strains gpb-2 grows rather poorly, especially if the plates are older so that there is a thick and tough lawn. On such plates there will be a lot of gpb-2 larval arrest, and those that don't arrest will grow slowly. The hets should easily outgrow the gpb-2 homozygotes. [gpb-2 is also hypersensitive to the drug arecoline: they won't grow on 5 mM. The hets will grow even better than WT on 5 mM arecoline.] gpb-2(ad541) previously called eat-11(ad541). Mutagen: EMS Outcrossed: x Made by: Duttweiler/Avery Received: 04/28/94 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA819 Species: Caenorhabditis elegans Genotype: eat-4(ad819) III. Description: Abnormal feeding. Mutagen: EMS Outcrossed: x2 Made by: R. Lee Received: 09/20/93 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA820 Species: Caenorhabditis elegans Genotype: eat-18(ad820) I. Description: Semidominant Eat. pka eat-18. Mutagen: EMS Outcrossed: x2 Made by: Raymond Lee Received: 07/17/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA823 Species: Caenorhabditis elegans Genotype: egl-30(ad805) I. Description: Suppressor of gpb-2 (a.k.a. eat-11) arecoline hypersensitivity. Unc. Egl. Mutagen: EMS Outcrossed: x Made by: Duttweiler/Avery Received: 09/20/93 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA837 Species: Escherichia coli Genotype: E. coli. Description: Bacteria. E. coli. Str-R. Uracil auxotroph. DA837 is derived from OP50. DA837 is harder for worms to eat than most E. coli strains. Some mild Eat mutant worms are easier to distinguish from WT when grown on DA837 than when grown on other E. coli strains. Biosafety Level: BSL-1. Mutagen: Outcrossed: x Made by: Leon Avery Received: 09/20/93 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA869 Species: Caenorhabditis elegans Genotype: sqt-3(sc8) lin-25(n545) him-5(e1467) unc-76(e911) V. Description: Roller. Unc. Vulvaless. Throws males. sc8 previously called rol-4(sc8). Mutagen: Outcrossed: x Made by: Leon Avery Received: 09/22/93 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA929 Species: Caenorhabditis elegans Genotype: egl-19(n582) unc-24(e138) IV. Description: Unc: amber suppressible. Egl: Lon, slow and floppy. Mutagen: Outcrossed: x Made by: Raymond Lee Received: 11/12/96 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA939 Species: Caenorhabditis elegans Genotype: unc-36(e251) III; egl-19(n2368) IV. Description: Unc. Semidominant Egl-c, Sma. Mutagen: Outcrossed: x Made by: Raymond Lee Received: 06/23/00 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA944 Species: Caenorhabditis elegans Genotype: unc-36(e251) III; egl-19(ad695) IV. Description: Unc. Semidominant Eat (TB relaxation defective). Mutagen: Outcrossed: x Made by: Raymond Lee Received: 06/23/00 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA945 Species: Caenorhabditis elegans Genotype: unc-36(e251) III; egl-19(n582) IV. Description: Unc. Semidominant Egl, Lon, Slow and Floppy Mutagen: Outcrossed: x Made by: Raymond Lee Received: 06/23/00 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA949 Species: Caenorhabditis elegans Genotype: bli-6(sc16) egl-19(n582) unc-24(e138) IV. Description: Blistered cuticle. Egl-Lon, slow and floppy. Unc. Mutagen: Outcrossed: x Made by: Raymond Lee Received: 07/20/95 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA952 Species: Caenorhabditis elegans Genotype: egl-19(n582ad952) IV. Description: n582 is Egl, Lon, slow & floppy. Suppressed by ad952, which is semidominant. Strain is slightly Dpy and the pharyngeal bulb occasionally shows delayed relaxation and repolarization. Mutagen: EMS Outcrossed: x Made by: Raymond Lee Received: 07/17/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA995 Species: Caenorhabditis elegans Genotype: egl-19(ad995) IV. Description: Lon. Sluggish Unc. Egl. Eat. Rubberband. Mutagen: EMS Outcrossed: x2 Made by: Raymond Lee Received: 07/17/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DA997 Species: Caenorhabditis elegans Genotype: eat-6(ad997) V. Description: Eat. Mutagen: EMS Outcrossed: x2 Made by: Joe Dent Received: 05/23/96 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DAG253 Species: C. elegans Genotype: lite-1(ce314) X; domEx253. Description: domEx253 [mec-4p::Chrimson::GFP + unc-122p::RFP]. Pick animals with red fluorescence in coelomocytes to maintain. Red-light optogenetic line for gentle touch receptor neurons (TRN). Transgenic animals expressing the red light-activated channelrhodopsin Chrimson into TRNs using the mec-4 promoter. In animals grown on all trans-retinal-containing medium, red light stimuli trigger behaviors similar to those evoked by gentle touch. Note that very strong blue light stimuli may also activate Chrimson. Reference: Schild LC & Glauser DA. Genetics. 2015 Aug;200(4):1029-34. doi: 10.1534/genetics.115.177956. PMID: 26022242. Mutagen: Outcrossed: x0 Made by: Lisa Schild Received: 12/01/22 Université de Fribourg, Fribourg, Switzerland ----------------------------------------------------------------------------- Strain: DAG261 Species: C. elegans Genotype: lite‐1(ce314) X; domEx261. Description: domEx261[mec‐4p::CoChR::GFP + unc‐122p::RFP]. Pick animals with red fluorescence in coelomocytes to maintain. High sensitivity blue-light optogenetic line for gentle touch receptor neurons (TRN). Transgenic animals expressing the high-sensitivity blue light-activated channelrhodopsin CoChR into TRNs using the mec-4 promoter. In animals grown on all trans-retinal-containing medium, low intensity blue light stimuli trigger behaviors similar to those evoked by gentle touch. Reference: Schild LC & Glauser DA. Genetics. 2015 Aug;200(4):1029-34. doi: 10.1534/genetics.115.177956. PMID: 26022242. Mutagen: Outcrossed: x0 Made by: Lisa Schild Received: 12/01/22 Université de Fribourg, Fribourg, Switzerland ----------------------------------------------------------------------------- Strain: DAG355 Species: C. elegans Genotype: lite‐1(ce314) X; domIs355. Description: domIs355 [mec‐3p::QF + mec‐4p::QS + QUAS::CoChR::GFP + unc122p::RFP]. High sensitivity blue-light optogenetic line for FLP neurons. Transgenic animals expressing the high-sensitivity blue light-activated channelrhodopsin CoChR into FLP using the Q-system combining mec-3p and mec-4p promoters. In animals grown on all trans-retinal-containing medium, low intensity blue light stimuli trigger reversal responses. Animals have red coelomocytes. The transgene was integrated with UV, and outcrossed 2x to parental ce314 mutant strain KG1180. Reference: Schild LC & Glauser DA. Genetics. 2015 Aug;200(4):1029-34. doi: 10.1534/genetics.115.177956. PMID: 26022242. Mutagen: UV Outcrossed: x2 Made by: Lisa Schild Received: 12/01/22 Université de Fribourg, Fribourg, Switzerland ----------------------------------------------------------------------------- Strain: DAW1 Species: Panagrolaimus sp. Genotype: Panagrolaimus sp. wild isolate Description: Antarctic nematode isolated from Ross Island, Antarctica in 1988. Panagrolaimus sp. DAW1 formerly known as Panagrolaimus davidi or P. davidi CB1. Survives freezing at -80 °C, including extensive intracellular freezing. Reference: Raymond MR, Wharton DA, Marshall CJ (2014) Antarc Sci 26:15-22. Mutagen: Outcrossed: x Made by: David Wharton Received: 02/12/16 University of Otago, Dunedin, New Zealand ----------------------------------------------------------------------------- Strain: DC1 Species: Caenorhabditis elegans Genotype: bah-1(br1) I. Description: Bah (biofilm absent on head - resistant to attachment of Yersinia sp. biofilms). Fragile cuticle (mild): increased sensitivity to alkaline-hypochlorite. Mutagen: ENU Outcrossed: x6 Made by: Creg Darby Received: 06/06/07 University of California, San Francisco, CA ----------------------------------------------------------------------------- Strain: DC1079 Species: Caenorhabditis elegans Genotype: ces-1(n703) qDf8/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT and GFP+ in the pharynx. qIs48 is an insertion of ccEx9747 (carries myo-2::GFP, pes-10::GFP, and a gut enhancer fused to GFP) onto the hT2 chromosome and is homozygous lethal. Presence of ces-1 is inferred from strain construction but not experimentally verified. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Mutagen: Outcrossed: x Made by: Creg Darby Received: 10/22/04 University of California, San Francisco, CA ----------------------------------------------------------------------------- Strain: DC19 Species: C. elegans Genotype: bus-5(br19) X. Description: Severe missense mutation. Bus (resistant to M. nematophilum), Bah (resistant to Yersinia biofilm formation), resistant to Leucobacter Verde2, hypersensitive to Leucobacter Verde1, drug and bleach sensitive. Slightly skiddy movement. Useful for drug screening. Reference: Xiong H, et al. Sci Rep. 2017 Aug 29;7(1):9839. Mutagen: EMS Outcrossed: x3 Made by: Creg Darby Received: 08/27/18 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: DC7 Species: Caenorhabditis elegans Genotype: bah-2(br7) IV. Description: Bah (biofilm absent on head - resistant to attachment of Yersinia sp. biofilms). Fragile cuticle (mild): increased sensitivity to alkaline-hypochlorite. Mutagen: ENU Outcrossed: x6 Made by: Creg Darby Received: 06/06/07 University of California, San Francisco, CA ----------------------------------------------------------------------------- Strain: DC9 Species: Caenorhabditis elegans Genotype: bah-3(br9) I. Description: Bah (biofilm absent on head - resistant to attachment of Yersinia sp. biofilms). Cld: constitutive larval display of epitope recognized by monoclonal antibody M37. Mutagen: ENU Outcrossed: x6 Made by: Creg Darby Received: 06/06/07 University of California, San Francisco, CA ----------------------------------------------------------------------------- Strain: DCD13 Species: Caenorhabditis elegans Genotype: uqIs9. Description: uqIs9 [myo-2p::rho1::tagRFP + tph-1p::GFP]. RHO-1::tagRFP aggregates in pharyngeal muscles. Animals have impaired pharyngeal pumping. Generated in N2 background. References: Huang YC, et al. Elife. 2019 May 3;8. pii: e43059. doi: 10.7554/eLife.43059. Mutagen: Outcrossed: x5 Made by: Della David Received: 10/01/19 Babraham Institute, Cambridge, UK ----------------------------------------------------------------------------- Strain: DCD146 Species: Caenorhabditis elegans Genotype: uqIs12 [myo-2p::rho-1::Venus]. Description: uqIs12 [myo-2p::rho-1::Venus]. RHO-1::VENUS aggregates in pharyngeal muscles. Generated in N2 background. References: Huang YC, et al. Elife. 2019 May 3;8. pii: e43059. doi: 10.7554/eLife.43059. Mutagen: Outcrossed: x4 Made by: Della David Received: 10/01/19 Babraham Institute, Cambridge, UK ----------------------------------------------------------------------------- Strain: DCD179 Species: Caenorhabditis elegans Genotype: uqEx37. Description: uqEx37 [kin-19p::kin-19::Venus + unc-122p::GFP]. Age-dependent aggregation of KIN-19::Venus in pharynx. Mutagen: Outcrossed: x0 Made by: Della David Received: 10/01/19 Babraham Institute, Cambridge, UK ----------------------------------------------------------------------------- Strain: DCD214 Species: C. elegans Genotype: uqIs24. Description: uqIs24 [myo-2p::tagRFP::pab-1]. Expression of tagRFP::PAB-1 in pharyngeal muscles; assembles into stress granules after heat shock (2 hr at 32C) and forms tagRFP::PAB-1 aggregates with age at 20C. Valuable marker for aging and for studying the aggregation of RNA-binding proteins with prion-like domains. Reference: Lechler MC, et al. Cell Rep. 2017 Jan 10;18(2):454-467. Mutagen: integrated by UV Outcrossed: x4 Made by: Della David Received: 11/13/17 Babraham Institute, Cambridge, UK ----------------------------------------------------------------------------- Strain: DCD23 Species: Caenorhabditis elegans Genotype: uqIs5. Description: uqIs5 [lbp-2p::lbp-2::TagRFP]. Age-dependent aggregation of LBP-2::TagRFP in the pseudocoelom. Reference: Gallotta I, et al. Nature. 2020 Jul 8. doi: 10.1038/s41586-020-2461-z. Mutagen: Outcrossed: x5 Made by: Della David Received: 07/27/20 Babraham Institute, Cambridge, UK ----------------------------------------------------------------------------- Strain: DCL569 Species: C. elegans Genotype: mkcSi13 II; rde-1(mkc36) V. Description: mkcSi13 [sun-1p::rde-1::sun-1 3'UTR + unc-119(+)] II. Germline rescue of the rde-1(mkc36) indel mutation, allowing germline-specific RNAi. Reference: Zou L, et al. Scientific Reports Volume 9, Article number: 2354 (2019) "Construction of a germline-specific RNAi tool in C. elegans." Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Lina Zou Received: 03/21/19 Nanjing University, Nanjing, China ----------------------------------------------------------------------------- Strain: DCR1017 Species: Caenorhabditis elegans Genotype: cima-1(wy84) IV; wyIs45 X; olaEx603. Description: wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx603 [rab-3p::cima-1 + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx605 does not rescue AIY presynaptic defects in cima-1(wy84) mutants. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. Mutagen: Outcrossed: x0 Made by: Zhiyong Shao Received: 06/14/14 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DCR1023 Species: Caenorhabditis elegans Genotype: cima-1(wy84) IV; wyIs45 X; olaEx609. Description: wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx609 [aex-3p::cima-1 + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx609 does not rescue AIY presynaptic defects in cima-1(wy84) mutants. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. Mutagen: Outcrossed: x0 Made by: Zhiyong Shao Received: 06/14/14 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DCR1078 Species: Caenorhabditis elegans Genotype: cima-1(wy84) IV; wyIs45 X; olaEx551. Description: wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx551 [cima-1p::cima-1 + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx551 rescues AIY presynaptic defects in cima-1(wy84) mutants. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. Mutagen: Outcrossed: x0 Made by: Zhiyong Shao Received: 06/14/14 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DCR1099 Species: Caenorhabditis elegans Genotype: cima-1(wy84) IV; wyIs45 X, olaEx644. Description: wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx644 [ttx-3p::cima-1 + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx642 does not rescue AIY presynaptic defects in cima-1(wy84) mutants. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. Mutagen: Outcrossed: x0 Made by: Zhiyong Shao Received: 06/14/14 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DCR1102 Species: Caenorhabditis elegans Genotype: cima-1(wy84) IV; wyIs45 X; olaEx647. Description: wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx647 [hlh-17p::cima-1 + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx647 does not rescue AIY presynaptic defects in cima-1(wy84) mutants. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. Mutagen: Outcrossed: x0 Made by: Zhiyong Shao Received: 06/14/14 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DCR1288 Species: Caenorhabditis elegans Genotype: cima-1(wy84) IV; wyIs45 X; olaEx765. Description: wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx765 [dpy-7p::cima-1 + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx765 rescues AIY presynaptic defects in cima-1(wy84) mutants. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. Mutagen: Outcrossed: x0 Made by: Zhiyong Shao Received: 06/14/14 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DCR1301 Species: Caenorhabditis elegans Genotype: cima-1(wy84) IV; wyIs45 X; olaEx775. Description: wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx775 [dpy-4p::cima-1 + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx605 rescues AIY presynaptic defects in cima-1(wy84) mutants. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. Mutagen: Outcrossed: x0 Made by: Zhiyong Shao Received: 06/14/14 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DCR1337 Species: Caenorhabditis elegans Genotype: nsIs105 I; cima-1(wy84) IV; wyIs45 X; olaEx805. Description: nsIs105 [hlh-17p::GFP] I. wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx805 [hlh-17p::caspase12 + hlh-17p::caspase17 + ttx-3p::mCherry + glr-3p::mCherry + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. Ablation of CEPsh glia partially suppresses AIY presynaptic maintenance defects in cima-1(wy84) mutants. nsIs105 (hlh-17::GFP) expression labels CEPsh glia. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. Mutagen: Outcrossed: x0 Made by: Zhiyong Shao Received: 06/14/14 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DCR1673 Species: Caenorhabditis elegans Genotype: olaEx987. Description: olaEx987 [ttx-3p::mCherry::rab-3 + hlh-17p::CD4::GFP1-10 + ttx-3p::CD4::GFP11 + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx987 labels AIY presynaptic sites with mCherry, and AIY and CEPsh contact with GFP. oleEx987 contains GRASP (GFP Reconstitution Across Synaptic Partners) constructs using two GFP fragments, GFP1-10 and GFP11, that can reconstitute a functional GFP molecule only when they are in close proximity. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. Mutagen: Outcrossed: x0 Made by: Zhiyong Shao Received: 06/14/14 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DCR1690 Species: Caenorhabditis elegans Genotype: cima-1(wy84) IV; egl-15(n484) wyIs45 X; olaEx1004. Description: wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx1004 [F25B3.3p::egl-15(5A) + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx1004 does not rescue egl-15(n484) suppression of cima-1 AIY presynaptic defects. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. Mutagen: Outcrossed: x0 Made by: Zhiyong Shao Received: 06/14/14 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DCR1710 Species: Caenorhabditis elegans Genotype: cima-1(wy84) IV; egl-15(n484) wyIs45 X; olaEx1015. Description: wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx1015 [dpy-7p::egl-15(5A) + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx1015 rescues egl-15(n484) suppression of cima-1 AIY presynaptic defects. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. Mutagen: Outcrossed: x0 Made by: Zhiyong Shao Received: 06/14/14 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DCR1779 Species: Caenorhabditis elegans Genotype: cima-1(wy84) IV; egl-15(n484) wyIs45 X; olaEx1054. Description: wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx1054 [dpy-7p::egl-15(5A)(ecto) + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx1054 rescues egl-15(n484) suppression of cima-1 AIY presynaptic defects. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. Mutagen: Outcrossed: x0 Made by: Zhiyong Shao Received: 06/14/14 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DCR2188 Species: Caenorhabditis elegans Genotype: olaEx1316. Description: olaEx1316 [ttx-3p::CD4::GFP11 + glr-3p::CD4::GFP1-10 + ttx-3p::mCherry::rab-3 + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx1316 labels AIY presynaptic sites with mCherry, and AIY and RIA contact with GFP. oleEx1316 contains GRASP (GFP Reconstitution Across Synaptic Partners) constructs using two GFP fragments, GFP1-10 and GFP11, that can reconstitute a functional GFP molecule only when they are in close proximity. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. Mutagen: Outcrossed: x0 Made by: Zhiyong Shao Received: 06/14/14 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DCR2335 Species: Caenorhabditis elegans Genotype: cima-1(wy84) IV; wyIs45 X; olaEx1411. Description: wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx1411 [dpy-7p::egl-15(5A)::HA + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. Mutagen: Outcrossed: x0 Made by: Zhiyong Shao Received: 06/14/14 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DCR3791 Species: Caenorhabditis elegans Genotype: pfk-1.1(ola72) X. Description: Diffuse distribution of synaptic vesicle markers (SNB-1, CAT-1, and RAB-3) under hypoxic conditions. pfk-1.1(ola72) causes C562Y missense mutation. Reference: Jang et al. Neuron. 2016 Apr 20;90(2):278-91. Mutagen: EMS Outcrossed: x2 Made by: SoRi Jang Received: 05/25/16 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DCR4521 Species: C. elegans Genotype: atg-9(ola274[atg-9::gfp]) V. Description: This endogenous tag on ATG-9 was made from a CRISPR event that used a method described by Dickinson et al. 2015 to efficiently insert GFP in replace of the stop codon of atg-9. Reference: Stavoe AK, et al. Dev Cell. 2016 Jul 25;38(2):171-85. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Sarah Hill Received: 04/25/19 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DCR4750 Species: C. elegans Genotype: olaIs35 X. Description: olaIs35 [ttx-3Gp::eGFP::lgg-1 + ttx-3Gp::mCherry + unc-122p::RFP]. Integrated transgene allows visualization of autophagosomes in a single neuron (AIY). "ttx-3G" refers to genomic fragment containing AIY motif described in Bertrand & Hobert Dev Cell. 2009 Apr;16(4):563-75. References: Stavoe AK, et al. Dev Cell. 2016 Jul 25;38(2):171-85. Hill SE, et al. Dev Cell. 2019 Mar 8. pii: S1534-5807(19)30104-2. Mutagen: UV/TMP Outcrossed: x5 Made by: Sarah Hill Received: 04/25/19 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DCR5765 Species: C. elegans Genotype: atg-4.2(ola316) IV. Description: Dominant negative allele of autophagy protease atg-4.2. Animals appear slightly dumpy. In neurons, autophagic vacuoles accumulate abnormally in the soma. Reference: Hill SE, et al. Dev Cell. 2019 Mar 8. pii: S1534-5807(19)30104-2. Mutagen: EMS Outcrossed: x3 Made by: Sarah Hill Received: 04/25/19 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DCR744 Species: Caenorhabditis elegans Genotype: cima-1(wy84) IV; wyIs45 X. Description: wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. AIY presynaptic maintenance defect at adult stage (synapses appears in asynaptic Zone 3 region). Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. Mutagen: EMS Outcrossed: x5 Made by: Zhiyong Shao Received: 06/14/14 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DCR8881 Species: C elegans Genotype: olaEx5329. Description: olaEx5329 [rab-3p::HYlight + elt-7p::mCherry]. Pick mCherry+ animals to maintain. Pan-neuronal expression of HYlight, a codon-optimized biosensor that responds to changing levels of FBP in cells. Reference: Wolfe AD, et al. Proc Natl Acad Sci U S A. 2024 Jan 16;121(3):e2314699121. doi: 10.1073/pnas.2314699121. PMID: 38198527. Mutagen: Outcrossed: x0 Made by: Aaron Wolfe Received: 07/08/24 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DCR8892 Species: C elegans Genotype: olaEx5331. Description: olaEx5331 [rab-3p::HYlight-RA + elt-7p::mCherry]. Pick mCherry+ animals to maintain. Pan-neuronal expression of HYlight-RA, a reduced affinity version of the FBP biosensor that does not respond to changes in concentration of the FBP metabolite during hypoxia in vivo. Can be used as a negative control for HYlight sensor in strain DCR8881. Reference: Wolfe AD, et al. Proc Natl Acad Sci U S A. 2024 Jan 16;121(3):e2314699121. doi: 10.1073/pnas.2314699121. PMID: 38198527. Mutagen: Outcrossed: x0 Made by: Aaron Wolfe Received: 07/08/24 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DCR9089 Species: C elegans Genotype: olaIs138 IV. Description: olaIs138 [ttx-3p::SL2::HYlight::let-858 3'UTR + elt-7p::mCherry] IV. Expression of HYlight, a codon-optimized biosensor that responds to changing levels of FBP in cells, in the neuron pair AIY. Derived by UV/TMP insertion of the olaEx5367 transgene. Reference: Wolfe AD, et al. Proc Natl Acad Sci U S A. 2024 Jan 16;121(3):e2314699121. doi: 10.1073/pnas.2314699121. PMID: 38198527. Mutagen: UV/TMP Outcrossed: x6 Made by: Aaron Wolfe, Milind Singh Received: 07/08/24 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DCR936 Species: Caenorhabditis elegans Genotype: cima-1(wy84) IV; wyIs45 X; olaEx559. Description: wyIs45 [ttx-3p::GFP::rab-3 + unc-122p::RFP] X. olaEx559 [rol-6p::cima-1 + unc-122p::GFP]. Maintain by picking animals with GFP expression in coelomocytes. olaEx559 rescues AIY presynaptic defects in cima-1(wy84) mutants. Reference: Shao Z, et al. Cell. 2013 Jul 18;154(2):337-50. Mutagen: Outcrossed: x0 Made by: Zhiyong Shao Received: 06/14/14 Yale University, New Haven, CT ----------------------------------------------------------------------------- Strain: DDP1 Species: Caenorhabditis elegans Genotype: uonEx1. Description: uonEx1 [unc-54::alpha-synuclein::CFP + unc-54::alpha-synuclein::YFP(Venus)]. No additional transformation marker was included in the array. uonEx1 also known as SC+SV in reference publications. Reduced lifespan (25-35% lower) and reduced pharyngeal pumping rate compared to N2. Novel transgenic strain for monitoring the influence of genetic and/or environmental factors on the extent of alpha-synuclein aggregation using FRET signals. Because the two fusion proteins are separate, FRET is only possible when synuclein aggregation brings a CFP tag very close to a YFP tag within an aggregate. We suggest using this strain in conjunction with the positive control (high FRET) strain DDP2 or with strain NL5901 which shows opposite changes in FRET. References: Nagarajan A, et al. CNS Neurol Disord Drug Targets. 2015 Aug 21. Bodhicharla R, et al. CNS Neurol Disord Drug Targets. 2012 Dec;11(8):965-75. Mutagen: Gamma radiation Outcrossed: x5 Made by: David de Pomerai Received: 09/29/15 University of Nottingham, Nottingham, UK ----------------------------------------------------------------------------- Strain: DDP2 Species: Caenorhabditis elegans Genotype: uonEx2. Description: uonEx2 [unc-54::CFP::YFP(Venus)]. No additional transformation marker was included in the array. uonEx2 also known as CV in reference publications. Lifespan and pharyngeal pumping rates are similar to N2. This is a high-FRET positive control strain for use in conjunction with DDP1. Because the CFP and YFP protein sequences are covalently fused together (and show little evidence of cleavage), FRET should be high and largely invariant since both fluorescent moieties are always in close proximity. References: Nagarajan A, et al. CNS Neurol Disord Drug Targets. 2015 Aug 21. Bodhicharla R, et al. CNS Neurol Disord Drug Targets. 2012 Dec;11(8):965-75. Mutagen: Gamma radiation Outcrossed: x5 Made by: David de Pomerai Received: 09/29/15 University of Nottingham, Nottingham, UK ----------------------------------------------------------------------------- Strain: DE112 Species: C. elegans Genotype: sup-46(qa710) I; dnSi4 II; unc-119(ed9) III. Description: dnSi4 [gna-1::GFP::gna-1 3’UTR + Cbr-unc-119(+)] inserted into ttTi5605 on LG II. Unknown if unc-119(ed9) III is homozygous or heterozygous in this strain. Mutagen: MosSCI Outcrossed: x0 Made by: Mark Sever Received: 05/28/17 Mount Sinai Hospital, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: DE115 Species: C. elegans Genotype: dnSi8 I; unc-119(ed3) III; dnIs22. Description: dnSi8 [tdp1::flag::mCherry + Cbr-unc119(+)] inserted into ttTi5605 on LG II. Nuclear-localized mCherry. dnIs22 [sup-46::GFP + unc-119(+)] (site of integration unknown). Strong nuclear-localized GFP expression. [NOTE: This strain was produced by crossing two parental strains carrying strong unc-119 loss of function alleles. One parent was carrying ed3; the allele in the other parental strain is unknown.] Mutagen: Bombardment and MosSCI Outcrossed: x0 Made by: Aldis Krizus Received: 05/28/17 Mount Sinai Hospital, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: DE116 Species: C. elegans Genotype: dnSi9II; unc-119(ed9) III. Description: dnSi9 [sup-46::GFP::sup-46 3’UTR + Cbr-unc-119(+)] inserted into ttTi5605 on LG II. Strong nuclear-localized GFP expression (expression from dnIs22 is brighter). Mutagen: MosSCI Outcrossed: x0 Made by: Aldis Krizus Received: 05/28/17 Mount Sinai Hospital, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: DE12 Species: C. elegans Genotype: sup-46(qa710) I. Description: Superficially WT. Suppressor of gna-2. Reduced brood counts at all temperatures (very strongly reduced at 26C). Exhibits mating-dependent progressive hermaphrodite sterility. Reduced embryo survival following heat shock. Mutagen: EMS Outcrossed: x10 Made by: Aldis Krizus Received: 05/28/17 Mount Sinai Hospital, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: DE130 Species: C. elegans Genotype: unc-119(e2488) III; dnIs24. Description: dnIs24 [sup46::flag::mCherry + Cbr-unc-119(+)]; site of integration unknown. Strong nuclear-localized mCherry. Mutagen: Bombardment Outcrossed: x0 Made by: Aldis Krizus Received: 05/28/17 Mount Sinai Hospital, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: DE83 Species: Caenorhabditis elegans Genotype: egg-1(ok1459) III. Description: Temperature sensitive embryonic lethal. Maintain at 15 C. 6% hatching rate at 25 C. Reference: Johnston et al. (2010) Curr Biol. Mutagen: Outcrossed: x5 Made by: Aldis Krizus Received: 10/20/10 Mount Sinai Hospital, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: DE90 Species: Caenorhabditis elegans Genotype: oxIs318 II; unc-119(ed3 or e2498) ruIs32 III; ddIs6 V; dnIs17. Description: oxIs318 [spe-11p::mCherry::histone + unc-119(+)] II. ruIs32 [pie-1p::GFP::histone H2B + unc-119(+)] III. ddIs6 [tbg-1::GFP + unc-119(+)] V. dnIs17 [pie-1p::GFP::hPLCIII?PH domain + unc-119(+)]. Maintain under normal conditions. Pick GFP+ to maintain. Reference: Johnston et al. (2010) Curr Biol. Mutagen: Outcrossed: x Made by: Aldis Krizus Received: 10/20/10 Mount Sinai Hospital, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: DF22 Species: Caenorhabditis elegans Genotype: tlp-1(bx85) unc-24(e138) IV; him-5(e1490) V. Description: Unc. Although hermaphrodites appear WT in other ways, there are some problems with T cell lineages (affecting the phasmids) and tail cell fusions. Variably Dyf. Male tail tip morphogenesis is also defective, resulting in blobby, "leptoderan" tails. Males are infertile due to an inability to properly copulate. Mutagen: Diethoxybutane Outcrossed: x Made by: Y Yang Received: 10/21/02 New York University, New York ----------------------------------------------------------------------------- Strain: DF5006 Species: Rhabditella axei Genotype: Rhabditella axei wild isolate. Description: 0006. Rhabditella axei. Male/Female strain. Isolated by W. Sudhaus on July 29, 1979 from a compost heap in Esmoulieres, France. Grown easily on OP50 at 16-25C. See WBG 12(5) 14. Mutagen: Outcrossed: x Made by: W. Sudhaus Received: 11/09/92 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: DF5010 Species: Metarhabditis blumi Genotype: Metarhabditis blumi wild isolate. Description: Metarhabditis blumi wild isolate. Formerly known as Rhabditis blumi. Isolated by J.P. Blum in April 1971 in a dung heap near Valencia, Spain. Gonochoristic. Grows well at 16-24C on OP50. Forms dauer larvae on overcrowded and starved plates. Freezes easily with C. elegans protocols with 80% viability. Mutagen: Outcrossed: x Made by: J.P. Blum Received: 08/29/94 New York University, New York ----------------------------------------------------------------------------- Strain: DF5012 Species: Rhomborhabditis regina Genotype: Rhomborhabditis regina wild isolate. Description: 0012. Male/Female strain. Isolated by M. Velasquez in the spring of 1989 from a June beetle larva (Scarabaeidae) near Quetzaltenango, Guatemala. WBG 12(5) 14. Formerly known as Rhabditoides regina. Mutagen: Outcrossed: x Made by: M. Velasquez Received: 11/09/92 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: DF5013 Species: Pelodera strongyloides Genotype: Pelodera strongyloides wild isolate. Description: 0013. Pelodera strongyloides. Male/Female strain. See WBG 12(5) 14. Originally came from Sudhaus. Mutagen: Outcrossed: x Made by: Received: 11/09/92 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: DF5017 Species: Mesorhabditis longespiculosa Genotype: Mesorhabditis longespiculosa wild isolate. Description: WT strain. Mesorhabditis longespiculosa. Isolated by W. Sudhaus on August 16, 1973 from tunnels of beetle larvae (Mallodon downesi?, Cerambycidae) in Euphorbiaceae at lake Nakuru, Kenya. See WBG 12(5) 14. Male/Female strain. Mutagen: Outcrossed: x Made by: W. Sudhaus Received: 12/29/92 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: DF5018 Species: Oscheius dolichuroides Genotype: Oscheius dolichuroides wild isolate. Description: WT strain. Rhabditis (Oscheius) dolichuroides. Isolated by W. Sudhaus in April 1978 from decaying matter in a hole in a tree in Malindi, Kenya. See WBG 12(5) 14. Male/Female strain. Mutagen: Outcrossed: x Made by: W. Sudhaus Received: 01/15/93 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: DF5019 Species: Teratorhabditis palmarum Genotype: Teratorhabditis palmarum wild isolate. Description: Robin Giblin-Davis MUST be cited in any publication. Isolated by R. Giblin-Davis from the palm weevil Rhynchphorus cruentatus (Curculionidae) in Broward County, Florida. Male/Female strain. See WBG 12(5) 14. Mutagen: Outcrossed: x Made by: Giblin-Davis R Received: 12/17/91 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: DF5020 Species: Oscheius myriophilus Genotype: Oscheius myriophilus wild isolate. Description: 0020. Formerly known as Rhabditis (Rhabditis) myriophila. Taxonomy ID: 281680 Isolated by G. O. Poiner (see Poiner, 1986). Mutagen: Outcrossed: x Made by: G. O. Poinar Received: 11/09/92 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: DF5022 Species: Pelodera strongyloides Genotype: Pelodera strongyloides wild isolate. Description: WT strain, Pelodera strongyloides. Isolated in April 1948 from pustules in the skin of a cow with dermatitis in central Illinois (see Levine et al 1950). Gonochoristic. May cause dermatitis in mammals (such as domesticated animals, and, rarely, humans). Grows well at 16-24C on OP50. Dauer larvae are the infective stage and are thermotactic. Freezes easily with C. elegans protocols with 70% viability. Previously called Pelodera strongyloides dermatitica by the CGC. Mutagen: Outcrossed: x Made by: W. Sudhaus Received: 08/29/94 New York University, New York ----------------------------------------------------------------------------- Strain: DF5025 Species: Reiterina typica Genotype: Reiterina typica wild isolate. Description: WT strain. Rhabditis (Pellioditis) typica. Male/Female strain. Isolated by W. Sudhaus on Sept. 3, 1973 from feces of an antelope in Tsavo National Park, Kenya. See WBG 12(5) 14. Formerly known as Rhabditis (Pellioditis) typica. Mutagen: Outcrossed: x Made by: W. Sudhaus Received: 01/15/93 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: DF5033 Species: Oscheius dolichura Genotype: Oscheius sp. wild isolate. Description: WT strain. Oscheius sp. See WBG 12(5) 14. Male/Female strain. Collected by a Belgian expedition to the Galapagos archipelago. From Gaetan Borgonie, Gent University, Belgium. Mutagen: Outcrossed: x Made by: K. Desender Received: 12/29/92 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: DF5040 Species: Pelodera strongyloides Genotype: Pelodera strongyloides wild isolate. Description: Male/Female strain. Pelodera strongyloides. See WBG 12(5) 14. Originally came from Victor Ambros. Mutagen: Outcrossed: x Made by: Received: 11/09/92 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: DF5070 Species: Caenorhabditis sp. 2 Genotype: Caenorhabditis sp. 2 wild isolate. Description: NOTE: To freeze this strain, heat shock at 37°C for 1 hr and add CaCl2 2mM in freezing solution. Mutagen: Outcrossed: x0 Made by: Karin Kiontke Received: 11/27/13 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: DF5081 Species: Caenorhabditis japonica Genotype: Caenorhabditis japonica. Description: Male-female strain. Maintain by mating. Strain used for sequencing. DF5081 is the strain of Caenorhabditis japonica that Karin Kiontke generated with 20x incrossing of DF5080. http://genome.wustl.edu/pub/organism/Invertebrates/Caenorha bditis_japonica/assembly/Caenorhabditis_japonica-3.0.2/ASSE MBLY Mutagen: Outcrossed: x Made by: Karin Kiontke Received: 01/09/09 New York University, New York ----------------------------------------------------------------------------- Strain: DF5112 Species: Caenorhabditis drosophilae Genotype: Caenorhabditis drosophilae wild isolate. Description: 20x inbred line derived from DF5077. Male-female strain. Heat shock 2.5-3.0 hrs at 30C immediately prior to freezing. Mutagen: Outcrossed: x20 Made by: Received: 06/15/16 New York University, New York ----------------------------------------------------------------------------- Strain: DF5152 Species: Caenorhabditis sp. 30 Genotype: Caenorhabditis sp. 30 wild isolate. Description: Isofemale line. Isolated from a rotten lemon collected at Chan Chich Lodge in the town of Gallon Jug, Orange Walk, Belize (17.56399°N, 89.046282°W) on 11/15/12. Mutagen: Outcrossed: x Made by: Received: 02/28/13 New York University, New York ----------------------------------------------------------------------------- Strain: DF5173 Species: Caenorhabditis sp. 8 Genotype: Caenorhabditis sp. 8 wild isolate. Description: Male-female strain. 20x inbred line derived from TMG13. Heat shock 2.5-3.0 hrs at 30C immediately prior to freezing. Mutagen: Outcrossed: x20 Made by: Received: 03/31/15 New York University, New York ----------------------------------------------------------------------------- Strain: DF64 Species: Caenorhabditis elegans Genotype: nyDf1(ny15) IV; him-5(e1490) V. Description: Deficiency covers at least all of cosmid T23G4; right breakpoint may be in cosmid C47A4. Fails to complement tlp-1(bx85). Viable and fertile. Although hermaphrodites appear WT in other ways, there are some problems with T cell lineages (affecting the phasmids) and tail cell fusions. Variably Dyf. Male tail tip morphogenesis is also defective, resulting in blobby, "leptoderan" tails. Males are infertile due to an inability to properly copulate. Mutagen: UV/TMP Outcrossed: x Made by: Y Yang Received: 10/21/02 New York University, New York ----------------------------------------------------------------------------- Strain: DG1255 Species: Caenorhabditis elegans Genotype: unc-53(e404) sqt-1(e1350) II. Description: e1350 is a recessive Dpy, dominant Roller. Unc. Mutagen: Outcrossed: x Made by: David Greenstein Received: 06/12/00 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG1575 Species: Caenorhabditis elegans Genotype: tnIs6. Description: tnIs6 [lim-7::GFP + rol-6(su1006)]. Rollers. lim-7::GFP is expressed in sheath cells (see Hall et al., 1999, Developmental Biology 212: 101-123. Insertion site not mapped. Mutagen: Gamma Outcrossed: x>1 Made by: Received: 04/25/03 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG1576 Species: Caenorhabditis elegans Genotype: tnIs5. Description: tnIs5 [lim-7::GFP + rol-6(su1006)]. Rollers. lim-7::GFP is expressed in sheath cells (see Hall et al., 1999, Developmental Biology 212: 101-123. Insertion site not mapped. Mutagen: Gamma Outcrossed: x>1 Made by: Received: 04/25/03 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG1604 Species: Caenorhabditis elegans Genotype: fog-2(q71) V; ceh-18(mg57) X. Description: Maintain by mating females with males. Mutagen: Outcrossed: x Made by: M Miller Received: 07/08/03 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG1612 Species: Caenorhabditis elegans Genotype: vab-1(dx31)/mIn1 [dpy-10(e128) mIs14] II; fog-2(q71) V. Description: Maintain by mating GFP+ females with GFP+ males. Mutagen: Outcrossed: x Made by: Michael Miller Received: 07/03/03 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG1650 Species: Caenorhabditis elegans Genotype: vab-1(dx31)/mIn1 [dpy-10(e128) mIs14] II; fog-2(q71) V; ceh-18(mg57) X. Description: Maintain by mating GFP+ females with GFP+ males. Mutagen: Outcrossed: x Made by: Michael Miller Received: 07/21/03 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG1701 Species: Caenorhabditis elegans Genotype: cgh-1(tn691) III. Description: Semi-dominant temperature-sensitive embryonic lethal. Maintain at 15C. Mutagen: EMS Outcrossed: x8 Made by: Greenstein/Yamamoto Received: 10/26/07 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG1770 Species: Caenorhabditis elegans Genotype: cgh-1(ok492) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: C07H6.5. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok492 homozygotes (sterile adult, tends to explode at vulva). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: GGCAGCTCGAAAATATTGCC. External right primer: GGAAAACCGCAAGGATGGTGG. Internal left primer: TCACGGAGCTAGATGTGACG. Internal right primer: CGTCAAAAAGAACCCGATGT. Internal WT amplicon: 3095 bp. Deletion size: 1043 bp. Deletion left flank: GAGAACATACACAATCTGGACGAGATCACT. Deletion right flank: CCTGGGGTGGCGATGACCAAGTGAACCGTT. This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. URL: http://www.celeganskoconsortium.omrf.org. Mutagen: UV/TMP Outcrossed: x>8 Made by: David Greenstein Received: 10/26/07 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG1856 Species: Caenorhabditis elegans Genotype: goa-1(sa734) I. Description: Recessive, early stop mutation within the coding sequence (C to T substitution in aa52) makes sa734 a likely null allele. May grow slightly better at 15C. Hyperactive, lays early stage eggs, increased amplitude of locomotory wave-form. Suppresses the lethargy and egg-laying defects of unc-43(n498). Reverses direction of locomotion more frequently than WT. Mutagen: EMS Outcrossed: x6 Made by: David Greenstein Received: 01/08/07 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG1867 Species: Caenorhabditis elegans Genotype: glp-1(tn777) III. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x Made by: S. Nadarajan Received: 12/17/10 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG2102 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; tnIs13 V. Description: tnIs13 [pie-1p::vab-1::GFP + unc-119(+)]. Mutagen: Outcrossed: x Made by: Hua Cheng Received: 05/05/08 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG2160 Species: Caenorhabditis elegans Genotype: tnIs13 ltIs44 V. Description: tnIs13 [pie-1p::vab-1::GFP + unc-119(+)]. ltIs44 [pie-1p::mCherry::PH(PLC1delta1) + unc-119(+)]. Mutagen: Outcrossed: x Made by: Hua Cheng Received: 05/05/08 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG2179 Species: Caenorhabditis elegans Genotype: tub-1(nr2044) II. Description: Mutagen: Tc1 Outcrossed: x Made by: Michael Basson Received: 09/25/07 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG2189 Species: Caenorhabditis elegans Genotype: fog-3(q443) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); tnIs13 ltIs44 V. Description: tnIs13 [pie-1p::vab-1::GFP + unc-119(+)]. ltIs44 [pie-1p::mCherry::PH(PLC1delta1) + unc-119(+)]. Homozygous hT2[bli-4 let-? qIs48] are inviable. Homozygous fog-3(q443) animals are females. Mutagen: Outcrossed: x Made by: Hua Cheng Received: 05/05/08 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG2190 Species: Caenorhabditis elegans Genotype: fog-3(q443) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); tnIs13 V. Description: tnIs13 [pie-1p::vab-1::GFP + unc-119(+)]. Homozygous hT2[bli-4 let-? qIs48] are inviable. Homozygous fog-3(q443) animals are females. Mutagen: Outcrossed: x Made by: Hua Cheng Received: 05/05/08 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG2356 Species: Caenorhabditis elegans Genotype: glp-1(tn777) III; fog-2(oz40) V. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x Made by: S. Nadarajan Received: 12/17/10 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG2361 Species: Caenorhabditis elegans Genotype: vab-2(e96) efn-2(ev658) IV; efn-3(ev696) X. Description: [NOTE: this strain is carrying vab-2(e96), not vab-2(e961)] Mutagen: Outcrossed: x Made by: Received: 01/22/08 Mt. Sinai Hospital Research Institute, Toronto, Ontario ----------------------------------------------------------------------------- Strain: DG2389 Species: Caenorhabditis elegans Genotype: glp-1(bn18) III. Description: Temperature sensitive. Maintain at 15C. Mutagen: EMS Outcrossed: x Made by: Susan Strome Received: 02/19/08 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG2474 Species: Caenorhabditis elegans Genotype: glp-1(ar202) III; fog-2(oz40) V. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x Made by: S. Nadarajan Received: 12/17/10 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG2884 Species: Caenorhabditis elegans Genotype: daf-2(e1370) III; fog-2(oz40) V. Description: Male-female strain. Maintain by mating at 15 C. Mutagen: Outcrossed: x Made by: David Greenstein Received: 02/04/10 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG2913 Species: Caenorhabditis elegans Genotype: egl-30(ad810) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ad810 homozygotes. Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Mutagen: Outcrossed: x Made by: A. Govinden Received: 05/12/10 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG2941 Species: Caenorhabditis elegans Genotype: spr-5(by134) I; acy-4(ok1806) V. Description: spr-5(by134) suppresses acy-4(ok1806) sterility. Reference: Kim S, et al. 2012 Genetics Mutagen: Outcrossed: x Made by: Seongseop Kim Received: 08/17/12 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG3095 Species: Caenorhabditis elegans Genotype: twk-1(tn1397) I; acy-4(ok1806) V. Description: twk-1(tn1397) suppresses acy-4(ok1806) sterility. acy-4(ok1806) suppresses twk-1(tn1397) adult-onset uncoordinated or paralyzed phenotype. Reference: Kim S, et al. 2012 Genetics Mutagen: EMS Outcrossed: x5 Made by: Seongseop Kim Received: 08/17/12 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG3226 Species: Caenorhabditis elegans Genotype: deps-1(bn124) I. Description: MAINTAIN AT 20 degrees C! deps-1 mutants are temperature-sensitive maternal-effect sterile (>80% sterile at 24.5C) and deps-1 M-Z- animals tend to lay many dead eggs and fewer eggs than WT at lower temps. Mutagen: Outcrossed: x Made by: Caroline Spike Received: 03/14/12 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG3373 Species: Caenorhabditis elegans Genotype: sacy-1(tn1385) I; acy-4(ok1806) V. Description: sacy-1(tn1385) suppresses acy-4(ok1806) sterility. Reference: Kim S, et al. 2012 Genetics Mutagen: Outcrossed: x5 Made by: Seongseop Kim Received: 08/17/12 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG3391 Species: Caenorhabditis elegans Genotype: acy-4(ok1806) V; tnEx37. Description: tnEx37 [acy-4(+) + sur-5::GFP]. Pick GFP+ animals to maintain. tnEx37 rescues acy-4(ok1806) sterility. Reference: Kim S, et al. 2012 Genetics Mutagen: Outcrossed: x Made by: Seongseop Kim Received: 08/17/12 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG3393 Species: Caenorhabditis elegans Genotype: kin-1(ok338) I; tnEx109. Description: tnEx109 [kin-1(+) + sur-5::GFP]. Segregates GFP+ fertile animals and GFP- kin-1(ok338) animals (early larval lethal). Reference: Kim S, et al. 2012 Genetics Mutagen: Outcrossed: x Made by: D. Greenstein Received: 08/17/12 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG3408 Species: Caenorhabditis elegans Genotype: twk-1(tn1397) I. Description: Adult-onset uncoordinated or paralyzed. DG3408 was segregated from DG3095 [twk-1(tn1397) I; acy-4(ok1806) V]. Reference: Kim S, et al. 2012 Genetics Mutagen: EMS Outcrossed: x>5 Made by: Seongseop Kim Received: 08/17/12 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG3430 Species: Caenorhabditis elegans Genotype: sacy-1(tn1385) I. Description: Superficial wild type. DG3430 was segregated from DG3373 [sacy-1(tn1385) I; acy-4(ok1806) V]. Reference: Kim S, et al. 2012 Genetics Mutagen: EMS Outcrossed: x>5 Made by: Seongseop Kim Received: 08/17/12 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG3449 Species: Caenorhabditis elegans Genotype: sacy-1(tn1385) I; fog-2(oz40) V. Description: sacy-1(tn1385) partially suppresses fog-2(oz40) self-sterility. Reference: Kim S, et al. 2012 Genetics Mutagen: Outcrossed: x>7 Made by: Seongseop Kim Received: 08/17/12 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG3485 Species: Caenorhabditis elegans Genotype: sacy-1(tm5503) I; tnEx159. Description: tnEx159 [sacy-1p::GFP::sacy-1 + unc-119(+)]. Pick wild-type to maintain. Transgene rescues sacy-1(tm5503) sterility. Reference: Kim S, et al. 2012 Genetics Mutagen: Outcrossed: x Made by: Seongseop Kim Received: 08/17/12 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG3492 Species: Caenorhabditis elegans Genotype: sacy-1(tm5503) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Segregates WT GFP+ heterozygotes, non-GFP sacy-1(tm5503) sterile animals, very rare GFP+ homozygous hT2, and dead eggs. Maintain by picking wild-type GFP+. Reference: Kim S, et al. 2012 Genetics Mutagen: Outcrossed: x5 Made by: Seongseop Kim Received: 08/17/12 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG3614 Species: Caenorhabditis elegans Genotype: inx-8(tn1474) inx-9(ok1502) IV; tnEx195. Description: tnEx195 [inx-8(+) + inx-9(+) + sur-5::GFP]. Pick GFP+ animals to maintain. Animals carrying tnEx195 are wild-type; animals that have lost the array are sterile and produce few germ cells. Reference: Starich TA, Hall DH, Greenstein D. Genetics. 2014 Nov;198(3):1127-53. Mutagen: EMS Outcrossed: x3 Made by: Todd Starich Received: 01/15/15 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG3784 Species: Caenorhabditis elegans Genotype: lin-41(tn1487) I. Description: Temperature-sensitive sterile. Maintain at 15C. Reference: Spike CA, et al. Genetics. 2014 Sep 26. pii: genetics.114.168831. Mutagen: EMS Outcrossed: x8 Made by: David Greenstein Received: 11/12/14 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG3887 Species: Caenorhabditis elegans Genotype: inx-21(tn1540) I/hT2[bli-4(e937) let-?(q782) qIs48] (I;III). Description: Homozygous sterile allele balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP sterile homozygotes (inx-21(tn1540) homozygous animals are sterile, producing few germ cells). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Starich TA, Hall DH, Greenstein D. Genetics. 2014 Nov;198(3):1127-53. Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Todd Starich Received: 01/15/15 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG3913 Species: Caenorhabditis elegans Genotype: lin-41(tn1541[GFP::tev::s::lin-41]) I. Description: lin-41(tn1541[GFP::tev::s::lin-41]) I. May be maintained under normal conditions. Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: David Greenstein Received: 10/30/14 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG3922 Species: Caenorhabditis elegans Genotype: tiar-1(tn1545[tiar-1::s::tev::GFP]) II. Description: tiar-1(tn1545[tiar-1::s::tev::GFP]) II. Reference: Huelgas-Morales G., et al. G3 (Bethesda). 2016 Apr; 6(4): 1031-1047. Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: G. Huelgas Morales Received: 05/20/16 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG3929 Species: Caenorhabditis elegans Genotype: tiar-1(tn1543[loxP::Cbr-unc-119(+)::loxP]) II. Description: tiar-1(tn1543[loxP::Cbr-unc-119(+)::loxP]) II. Reference: Huelgas-Morales G., et al. G3 (Bethesda). 2016 Apr 7;6(4):1031-47. Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: G. Huelgas Morales Received: 06/20/16 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG4153 Species: Caenorhabditis elegans Genotype: pod-2(tn1691) II; tnEx212. Description: tnEx212 [pod-2(+) + sur-5::GFP]. Pick GFP+ animals to maintain. sur-5::gfp(+) animals are wild type and segregate GFP(+) wild-type animals and GFP(-) pod-2(tn1691) dead embryos. tn1691 deletes ~15 kb within pod-2, including most of Exon 2 through to and including the stop codon (but not the polyA site). Reference: Starich TA, et al. eLife 2020;9:e58619 DOI: 10.7554/eLife.58619 PMID: 32735213 Mutagen: CRISPR-Cas9 Outcrossed: x2 Made by: Todd Starich Received: 02/08/21 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG4158 Species: C. elegans Genotype: spn-4(tn1699[spn-4::gfp::3xflag]) V. Description: Apparently wild type Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Tatsuya Tsukamoto Received: 07/03/17 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG4190 Species: C. elegans Genotype: cdc-25.3(tn1712[gfp::3xflag::cdc-25.3]) III. Description: Superficially wild type Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Tatsuya Tsukamoto Received: 07/03/17 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG4213 Species: C. elegans Genotype: meg-1(tn1724[gfp::3xflag::meg-1]) X. Description: Superficially wild type Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Tatsuya Tsukamoto Received: 07/03/17 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG4215 Species: C. elegans Genotype: puf-5(tn1726[gfp::3xflag::puf-5]) II. Description: Superficially wild type Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Tatsuya Tsukamoto Received: 07/03/17 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG4218 Species: C. elegans Genotype: cpg-1(tn1728[mNG::3xflag::cpg-1]) III. Description: mNeonGreen and Flag tags inserted at 5' end of endogenous cpg-1 locus. Superficially wild type. Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Tatsuya Tsukamoto Received: 07/03/17 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG4222 Species: C. elegans Genotype: pos-1(tn1730[gfp::3xflag::pos-1]) V. Description: Superficially wild type Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Tatsuya Tsukamoto Received: 07/03/17 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG4228 Species: C. elegans Genotype: orc-1(tn1732[mNeonGreen::3xflag::orc-1]) III. Description: Superficially wild type Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Tatsuya Tsukamoto Received: 07/03/17 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG4230 Species: C. elegans Genotype: gla-3a(tn1734[gfp::3xflag::gla-3a]) I. Description: Superficially wild type Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Tatsuya Tsukamoto Received: 07/03/17 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG4233 Species: C. elegans Genotype: pqn-59(tn1737[gfp::3xflag::pqn-59]) I. Description: Superficially wild type Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Tatsuya Tsukamoto Received: 07/03/17 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG4269 Species: C. elegans Genotype: mex-3(tn1753[gfp::3xflag::mex-3]) I. Description: Superficially wild type Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Tatsuya Tsukamoto Received: 07/03/17 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG4324 Species: C. elegans Genotype: pod-2(tn1765[gfp::3xflag::pod-2]) II. Description: Homozygous viable, gfp expression in intestine, hypodermis, somatic gonad, excretory duct, CAN neuron. Reference: Starich et al. eLife 2020;9:e58619. DOI: https://doi.org/10.7554/eLife.58619 Mutagen: Outcrossed: x3 Made by: Todd Starich Received: 03/21/23 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG4329 Species: Caenorhabditis elegans Genotype: fasn-1(tn1762) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III) Description: Homozygous lethal deletion balanced by bli-4- and GFP-marked translocation. Heterozygotes are wild-type with pharyngeal GFP signal, and segregate wild-type GFP heterozygotes, arrested hT2 aneuploids, and non-GFP tn1762 homozygotes (dead embryos and L1 larvae). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick wild-type GFP and check for correct segregation of progeny to maintain. tn1762 is a ~9.2 kb deletion within fasn-1, including Exon 2 thru to 57 nt preceding stop codon. Reference: Starich TA, et al. eLife 2020;9:e58619 DOI: 10.7554/eLife.58619 PMID: 32735213 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Todd Starich Received: 02/08/21 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG4384 Species: Caenorhabditis elegans Genotype: hmgr-1(tm4386) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I; III). Description: Heterozygotes are WT, GFP+ in the pharynx and segregate WT (GFP+ in the pharynx), dead eggs (homozygotes for hT2) and hmgr-1(tm4386) homozygotes, which die as young larvae (L1/L2). Maintain by picking GFP+ worms and checking for correct segregation, since the hT2 balancer is lost at low frequencies. hmgr-1(tm4386) can be maintained as homozygous fertile hermaphrodites on 20 mM mevalonolactone. Reference: P. Ranji, M. Rauthan, C. Pitot and M. Pilon, 2014. PloS ONE 9(6): e100033. Mutagen: UV/TMP Outcrossed: x>10 Made by: Todd Starich Received: 03/28/17 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG4392 Species: C. elegans Genotype: cyb-3(tn1755[gfp::3xflag::cyb-3]) V. Description: Although this strain is maintainable as a homozygote it produces many dead embryos (~80%) and has a low viable brood size (~24 ± 18). Thus, the GFP tag compromises CYB-3 function. Mutagen: CRISPR-Cas9 Outcrossed: x2 Made by: Tatsuya Tsukamoto Received: 07/03/17 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG4454 Species: C. elegans Genotype: npp-12(ok2424) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III) Description: Homozygous ok2424 animal are viable and fertile, but will go sterile in successive generations. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok2424 homozygotes (superficially wild-type with some sterility). Homozygous hT2[bli-4 let-? qIs48] inviable. Maintain by picking GFP+ heterozygotes and checking for correct segregation of progeny to maintain a balanced stock. Derived from parental strain RB1874, originally provided to the CGC by the OMRF Knockout Group, part of the International C. elegans Gene Knockout Consortium. Paper_evidence WBPaper00041807 Mutagen: Outcrossed: x1 Made by: Gabriela Huelgas Morales Received: 11/09/17 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG4569 Species: Caenorhabditis elegans Genotype: cyb-1(tn1806[cyb-1::gfp::tev::3xflag]) IV. Description: Viable and fertile, grows and moves well. No apparent abnormalities yet noted. Reference: Spike CA, et al. Genetics. 2022 May 5;221(1):iyac051. doi: 10.1093/genetics/iyac051. PMID: 35377419 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Tatsuya Tsukamoto Received: 06/27/22 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG627 Species: Caenorhabditis elegans Genotype: emb-30(tn377) III. Description: Temperature sensitive. Maintain at 15C. Male mating stock. Mutagen: EMS Outcrossed: x7 Made by: David Greenstein Received: 01/03/00 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG695 Species: Caenorhabditis elegans Genotype: +/hT2 [dpy-18(h662)] I; unc-36(e251) evl-8(ar102)/hT2 [bli-4(e937)] III. Description: Heterozygotes are WT and segregate WT, Uncs with an everted Vulva, Dpys and dead eggs. Mutagen: Outcrossed: x Made by: David Greenstein Received: 09/08/97 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG696 Species: Caenorhabditis elegans Genotype: +/hT2 [dpy-18(h662)] I; unc-36(e251) evl-19(ar98)/hT2 [bli-4(e937)] III. Description: Heterozygotes are WT and segregate WT, Uncs with an everted Vulva, Dpys and dead eggs. Mutagen: Outcrossed: x Made by: David Greenstein Received: 09/08/97 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG728 Species: Caenorhabditis elegans Genotype: sma-2(e502) emb-30(tn377) ced-7(n1892) unc-69(e587) III. Description: Temperature sensitive emb-30 allele. Maintain at 15C. Small. Unc. Cell death abnormal. Mutagen: Outcrossed: x Made by: David Greenstein Received: 01/03/00 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG753 Species: Caenorhabditis elegans Genotype: emb-30(tn474) III. Description: Allele viable at all temperatures (15-25C). Mutagen: EMS Outcrossed: x7 Made by: David Greenstein Received: 01/03/00 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG769 Species: Caenorhabditis elegans Genotype: unc-32(e189) emb-30(tn476) III/eT1 (III;V). Description: Heterozygotes are WT and segregate WT, Unc-32 Steriles and Unc-36. Mutagen: EMS Outcrossed: x7 Made by: David Greenstein Received: 01/03/00 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG783 Species: Caenorhabditis elegans Genotype: unc-32(e189) emb-30(tn478) III/eT1 (III;V). Description: Heterozygotes are WT and segregate WT, Unc-32 Steriles and Unc-36. Mutagen: EMS Outcrossed: x7 Made by: David Greenstein Received: 01/03/00 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG784 Species: Caenorhabditis elegans Genotype: unc-32(e189) emb-30(tn477) III/eT1 (III;V). Description: Heterozygotes are WT and segregate WT, Unc-32 maternal effect lethals (Mel) and Unc-36. Mutagen: EMS Outcrossed: x7 Made by: David Greenstein Received: 01/03/00 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG786 Species: Caenorhabditis elegans Genotype: unc-32(e189) emb-30(tn480) III/eT1 (III;V). Description: Heterozygotes are WT and segregate WT, Unc-32 Steriles and Unc-36. Mutagen: EMS Outcrossed: x7 Made by: David Greenstein Received: 01/03/00 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG796 Species: Caenorhabditis elegans Genotype: sma-2(e502) tnDf2/sma-2(e502) ced-7(n1892) unc-69(e587) III. Description: Heterozygotes are SmaCed and segregate SmaCed, SmaCedUnc and dead eggs. Maintain by picking SmaCed. tnDf2 is not transmitted well by males (i.e. tnDf2/+ males have a low mating efficiency). Mutagen: TMP + UV Outcrossed: x Made by: Received: 09/03/98 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG800 Species: Caenorhabditis elegans Genotype: unc-32(e189) tnDf2 III/eT1 (III;V). Description: Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. tnDf2 is not transmitted well by males (i.e. tnDf2/+ males have a low mating efficiency). Mutagen: TMP + UV Outcrossed: x Made by: Received: 09/15/98 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG801 Species: Caenorhabditis elegans Genotype: unc-32(e189) tnDf2/sma-2(e502) ced-7(n1892) unc-69(e587) III. Description: Heterozygotes are Ced and segregate Ced, dead eggs and SmaCedUncs. Maintain by picking Ced. tnDf2 is not transmitted well by males (i.e. tnDf2/+ males have a low mating efficiency). Mutagen: TMP + UV Outcrossed: x Made by: Received: 09/15/98 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG815 Species: Caenorhabditis elegans Genotype: unc-32(e189) emb-30(tn475)/unc-36(e251) III. Description: Heterozygotes are WT and segregate WT, Unc-32 Steriles and Unc-36. Mutagen: EMS Outcrossed: x7 Made by: David Greenstein Received: 01/03/00 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG832 Species: Caenorhabditis elegans Genotype: emb-30(tn475)/eT1 III; unc-46(e177) mdf-1(gk2)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-46 Steriles, and Unc-36 (eT1 homozygotes). Maintain by picking WT. Mutagen: EMS Outcrossed: x7 Made by: Received: 09/12/05 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DG844 Species: Caenorhabditis elegans Genotype: emb-30(tn377) III; unc-46(e177) mdf-1(gk2) V. Description: emb-30(tn377ts) conditionally suppresses mdf-1(gk2). Maintain at 15C. Mutagen: EMS Outcrossed: x7 Made by: Received: 09/12/05 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DH1 Species: Caenorhabditis elegans Genotype: zyg-1(b1) II. Description: Temperature sensitive. Embryonic lethal at 25C (tight); arrests at first cleavage. Gondadogenesis defective. Will grow at 15C or 20C. Maternal effect (m,m). Mutagen: EMS Outcrossed: x2 Made by: Vanderslice R Received: 12/01/80 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DH10 Species: Caenorhabditis elegans Genotype: mus-101(b10) I. Description: Temperature sensitive. Embryonic lethal. Gonadogenesis abnormal. Recessive. Maternal effect (m,m). Strict. Does not grow at 20C or 25C. Mutagen: EMS Outcrossed: x2 Made by: Vanderslice R Received: 12/01/80 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DH1033 Species: Caenorhabditis elegans Genotype: sqt-1(sc103) II; bIs1 X. Description: bIs1[vit-2::GFP + rol-6(su1006)]. Mutagen: Outcrossed: x Made by: Barth Grant Received: 02/15/06 Rutgers University, Piscataway, NJ ----------------------------------------------------------------------------- Strain: DH117 Species: Caenorhabditis elegans Genotype: emb-9(b117) III. Description: Temperature sensitive. Semi-dominant. Egg lethal. Not maternal. Will grow at 20C. Mutagen: EMS Outcrossed: x2 Made by: Vanderslice R Received: 12/01/80 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DH1201 Species: Caenorhabditis elegans Genotype: rme-1(b1045) V. Description: Viable. Progressive vacuolation of intestine beginning in L4. Endocytosis defects in oocytes and coelomocytes. Mutagen: UV/TMP Outcrossed: x2 Made by: Barth Grant Received: 03/02/02 Rutgers University, Piscataway, NJ ----------------------------------------------------------------------------- Strain: DH1206 Species: Caenorhabditis elegans Genotype: rme-8(b1023) I. Description: Temperature sensitive lethal. Maintain at 15C. Endocytosis defects in oocytes (non-ts) and coelomocytes (ts). Mutagen: ENU Outcrossed: x3 Made by: Barth Grant Received: 03/02/02 Rutgers University, Piscataway, NJ ----------------------------------------------------------------------------- Strain: DH1230 Species: Caenorhabditis elegans Genotype: chc-1(b1025) III. Description: Temperature sensitive lethal. Endocytosis defective. Maintain at 15C. AKA: rme-3. Mutagen: EMS Outcrossed: x3 Made by: Barth Grant Received: 01/27/09 Rutgers University, Piscataway, NJ ----------------------------------------------------------------------------- Strain: DH126 Species: Caenorhabditis elegans Genotype: zyg-13(b126) IV. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x Made by: Received: 01/27/95 University of Houston, TX ----------------------------------------------------------------------------- Strain: DH1300 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: DH subclone of C. briggsae Zuckerman. This stock was maintained in liquid culture for some number of years, and has acquired mutations that have not been named or mapped. It is Unc, dauer-defective and ts lethal. Previously called C. briggsae BO. Mutagen: Outcrossed: x Made by: Received: 01/01/80 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DH1336 Species: Caenorhabditis elegans Genotype: bIs34. Description: bIs34 [rme-8::GFP + rol-6(su1006)]. Mutagen: Outcrossed: x Made by: Yinhua Zhang Received: 02/15/06 Rutgers University, Piscataway, NJ ----------------------------------------------------------------------------- Strain: DH1370 Species: Caenorhabditis elegans Genotype: rme-6(b1014) X. Description: Accumulation of yolk GFP in pseudocoelom suggesting decrease or absence of coelomocyte mediated endocytosis. Abundant pseudocoelomic yolk. Mutagen: EMS Outcrossed: x3 Made by: Barth Grant Received: 01/24/06 Rutgers University, Piscataway, NJ ----------------------------------------------------------------------------- Strain: DH1390 Species: Caenorhabditis elegans Genotype: rme-2(b1008) IV. Description: Low brood size and incompletely penetrant embryonic lethality. Accumulates yolk in the pseudocoelom of adult hermaphrodites. Males are normal. Mutagen: Tc1 Outcrossed: x3 Made by: Barth Grant Received: 10/31/00 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DH18 Species: Caenorhabditis elegans Genotype: zyg-3(b18) II. Description: Temperature sensitive. Embryonic lethal. Semi-dominant. Gonadogenesis defective. maternal effect (m,n). Will grow at 20C, but not 25C. Mutagen: EMS Outcrossed: x2 Made by: Vanderslice R Received: 12/01/80 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DH187 Species: Caenorhabditis elegans Genotype: zyg-7(b187) III. Description: Temperature sensitive. Egg lethal. Will grow at 20C, but not 25C. Recessive. Maternal effect (m,n). Mutagen: EMS Outcrossed: x2 Made by: Carr S Received: 12/01/80 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DH189 Species: Caenorhabditis elegans Genotype: emb-9(b189) III. Description: Temperature sensitive. Egg lethal. Maternal effect (m,n). Acc and Gon. Some growth at 20C, but not at 25C. Mutagen: EMS Outcrossed: x Made by: Carr S Received: 03/01/82 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DH2 Species: Caenorhabditis elegans Genotype: zyg-11(b2) II. Description: Temperature sensitive. Leaky. Penetrance incomplete. Semi-dominant. Cleavage abnormal. Maternal effect(m,m). Received new stock from Edward Kipreos 7/2003. Mutagen: EMS Outcrossed: x Made by: Vanderslice R Received: 12/01/80 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DH202 Species: Caenorhabditis elegans Genotype: tra-2(b202) II. Description: Temperature sensitive. XX animals are self-fertile hermaphrodites at 15C. XX animals are sterile males at 25C. ts period begins in late embryo. Mutagen: EMS Outcrossed: x Made by: Carr S Received: 12/01/80 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DH235 Species: Caenorhabditis elegans Genotype: zyg-8(b235) III. Description: Temperature sensitive. Egg lethal. Abnormal first cleavage. Maternal effect (m,n). Mutagen: EMS Outcrossed: x2 Made by: Carr S Received: 12/01/80 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DH238 Species: Caenorhabditis elegans Genotype: sqt-3(b238) V. Description: Temperature sensitive roller. PKA rol-4. Mutagen: Outcrossed: x Made by: Received: 12/01/80 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DH244 Species: Caenorhabditis elegans Genotype: zyg-9(b244) II. Description: Temperature sensitive. Egg lethal. Abnormal first cleavage. Strict maternal effect (m,m). Will grow at 20C. Mutagen: EMS Outcrossed: x2 Made by: Carr S Received: 12/01/80 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DH245 Species: Caenorhabditis elegans Genotype: fem-2(b245) III. Description: Temperature sensitive. XX animals make no sperm. Temperature sensitive period in L1. Maternal effect. XO animals are intersexual males. Temperature sensitive period in soma is L1-L3. Temperature sensitive period in germline is L2-adult. Mutagen: EMS Outcrossed: x2 Made by: D. Hirsh Received: 03/01/85 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: DH246 Species: Caenorhabditis elegans Genotype: let-2(b246) X. Description: Temperature sensitive embryonic lethal. Grows at 15C, 20C. Lethal at 25C (embryonic). See also CGC 1806. Mutagen: Outcrossed: x Made by: Received: 04/01/86 University of Colorado, Boulder ----------------------------------------------------------------------------- Strain: DH26 Species: Caenorhabditis elegans Genotype: rrf-3(b26) II. Description: Temperature sensitive. Spermatogenesis defective. Rescue by mating with WT male. Sterile at 25C. Mutagen: EMS Outcrossed: x2 Made by: Vanderslice R Received: 12/01/80 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DH261 Species: Caenorhabditis elegans Genotype: zyg-10(b261) III. Description: Temperature sensitive. Egg lethal. Abnormal first cleavage giving small P1 blastomere. Mutant is ts in late L4-early adult. Partial maternal (m,n). Mutagen: EMS Outcrossed: x2 Made by: Hirsh D Received: 12/01/80 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DH404 Species: Caenorhabditis elegans Genotype: unc-63(b404) I. Description: Unc. Trichlorfon resistant. Lev resistance leaky. Mutagen: EMS Outcrossed: x Made by: Carr S Received: 02/01/82 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DH424 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Interfertile with N2. Survives freezing lin liquid nitrogen. Temperature sensitive. Brood size like N2. Generation time like N2. Spontaneous males. Reference WBG 10(2) 140-141. Isolated in El Prieto Canyon, CA. Caenorhabditis elegans wild isolate (Tc1 pattern HCC). Mutagen: Outcrossed: x Made by: L. Liao Received: 09/01/82 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DH84 Species: Caenorhabditis elegans Genotype: emb-7(b84) III. Description: Temperature sensitive. Egg lethal. Gon phenotype if shifted to 25C early. Maternal effect (m,m). Mutagen: EMS Outcrossed: x Made by: Vanderslice R Received: 02/01/82 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DH89 Species: Caenorhabditis elegans Genotype: zyg-5(b89) II. Description: Temperature senstive. No growth at 20C or 25C. Weakly semidominant. Egg lethal. Mutagen: EMS Outcrossed: x2 Made by: Vanderslice R Received: 12/01/80 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DL160 Species: Caenorhabditis elegans Genotype: lin-59(n3192) unc-29(e1072) I. Description: Mutagen: Outcrossed: x Made by: Jennifer Winn Received: 06/13/11 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DL200 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Reference: Andersen EC, et al. Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: Dee Denver Received: 09/04/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: DL220 Species: Caenorhabditis elegans Genotype: dpy-20(e1282) mig-32(tm1684) IV. Description: Mutagen: Outcrossed: x0 Made by: Jennifer Winn Received: 06/13/11 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DL226 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Reference: Andersen EC, et al. Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: C. Hilburn Received: 09/04/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: DL238 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Reference: Andersen EC, et al. Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: J. Knapp Received: 09/04/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: DL308 Species: Caenorhabditis elegans Genotype: ced-3(n717) IV; mxIs28 X. Description: mxIs28 [ceh-20p::ceh-20::YFP + lin-15(+)] X. Reference: Potts MB, Wang DP, & Cameron S. Dev Biol. 2009 May 15;329(2):374-85. Mutagen: UV Outcrossed: x9 Made by: Malia Potts Received: 03/25/11 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DL315 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; mxIs30. Description: mxIs30 [unc-62p::unc-62::CFP + lin-15(+)]. Reference: Potts MB, Wang DP, Cameron S. Dev Biol. 2009 May 15;329(2):374-85. Mutagen: Outcrossed: x Made by: Scott Cameron Received: 04/06/11 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DL7 Species: Caenorhabditis elegans Genotype: wdIs4 II; unc-3(n3435) X. Description: wdIs4 [unc-4p::GFP + dpy-20(+)] II. Mutagen: Outcrossed: x Made by: Jennifer Winn Received: 06/13/11 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DL74 Species: Caenorhabditis elegans Genotype: mig-32(n4275) IV. Description: Mutagen: Outcrossed: x8 Made by: Jennifer Winn Received: 06/13/11 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: DLM1 Species: Caenorhabditis elegans Genotype: ttTi5605 II; unc-119(ed3) III; uwaEx1. Description: uwaEx1 [eft-3p::CERULEAN-VENUS::lgg-1 + unc-119(+)]. Pick non-Unc to maintain. Ubiquitously-expressed lgg-1 tagged with oxCerulean-oxVenus double fluorescent protein (dFP) for monitoring autophagy. Reference: Chapin H, et al. Aging (Albany NY). 2015 Jun;7(6):419-34. Mutagen: Outcrossed: x0 Made by: Hannah Chapin Received: 08/21/15 University of Washington ----------------------------------------------------------------------------- Strain: DLM10 Species: Caenorhabditis elegans Genotype: uwaSi5 II; unc-119(ed3) III. Description: uwaSi5 [myo-3p::CERULEAN-VENUS::lgg-1 + unc-119(+)] II. MosSCI insertion into ttTi5605 II. lgg-1 tagged with oxCerulean-oxVenus double fluorescent protein (dFP) for monitoring autophagy in body wall muscle. Reference: Chapin H, et al. Aging (Albany NY). 2015 Jun;7(6):419-34. Mutagen: MosSCI Outcrossed: x0 Made by: Hannah Chapin Received: 08/21/15 University of Washington ----------------------------------------------------------------------------- Strain: DLM11 Species: Caenorhabditis elegans Genotype: ttTi5605 II; unc-119(ed3) III; uwaEx6. Description: uwaEx6 [rab-3p::CERULEAN-VENUS::lgg-1 + unc-119(+)]. Pick non-Unc to maintain. lgg-1 tagged with oxCerulean-oxVenus double fluorescent protein (dFP) for monitoring autophagy in neurons. Reference: Chapin H, et al. Aging (Albany NY). 2015 Jun;7(6):419-34. Mutagen: Outcrossed: x0 Made by: Hannah Chapin Received: 08/21/15 University of Washington ----------------------------------------------------------------------------- Strain: DLM13 Species: Caenorhabditis elegans Genotype: ttTi5605 II; unc-119(ed3) III; uwaEx7. Description: uwaEx8 [eft-3p::CERULEAN-VENUS + unc-119(+)]. Pick non-Unc to maintain. Ubiquitously-expressed oxCerulean-oxVenus double fluorescent protein (dFP) for monitoring autophagy. Reference: Chapin H, et al. Aging (Albany NY). 2015 Jun;7(6):419-34. Mutagen: Outcrossed: x0 Made by: Hannah Chapin Received: 08/21/15 University of Washington ----------------------------------------------------------------------------- Strain: DLM14 Species: Caenorhabditis elegans Genotype: ttTi5605 II; unc-119(ed3) III; uwaEx8. Description: uwaEx8 [eft-3p::CERULEAN-VENUS::tomm-7 + unc-119(+)]. Pick non-Unc to maintain. Ubiquitously-expressed tomm-7 tagged with oxCerulean-oxVenus double fluorescent protein (dFP) for monitoring autophagy. Reference: Chapin H, et al. Aging (Albany NY). 2015 Jun;7(6):419-34. Mutagen: Outcrossed: x0 Made by: Hannah Chapin Received: 08/21/15 University of Washington ----------------------------------------------------------------------------- Strain: DLM15 Species: Caenorhabditis elegans Genotype: ubc-18(tm5426) III. Description: Mutagen: Outcrossed: x1 Made by: Hilary Kemp Received: 03/15/25 University of Washington ----------------------------------------------------------------------------- Strain: DLM16 Species: Caenorhabditis elegans Genotype: ubc-18(tm5426) sup-35(e2215) pha-1(e2123) III. Description: sup-35 rescues synthetic lethality of ubc-18 and pha-1. Mutagen: Outcrossed: x0 Made by: Hilary Kemp Received: 03/15/25 University of Washington ----------------------------------------------------------------------------- Strain: DLM17 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; sup-36(e2217) IV. Description: Superficially wild-type. sup-36 suppresses temperature-sensitive lethality of pha-1(e2123). Constructed by crossing GE24 and MT14541. Mutagen: Outcrossed: x0 Made by: Hilary Kemp Received: 03/15/25 University of Washington ----------------------------------------------------------------------------- Strain: DLM18 Species: Caenorhabditis elegans Genotype: ubc-18(tm5426) III; sup-36(e2217) IV. Description: sup-36 suppresses synthetic lethality and Pun phenotype of ubc-18(tm5426) animals grown on ubc-3 RNAi. Mutagen: Outcrossed: x0 Made by: Hilary Kemp Received: 03/15/25 University of Washington ----------------------------------------------------------------------------- Strain: DLM19 Species: Caenorhabditis elegans Genotype: ubc-18(tm5426) III; sup-37(e2215) V. Description: DLM 19: sup-37 suppresses synthetic lethality and Pun phenotype of ubc-18(tm5426) animals grown on ubc-3 RNAi. Mutagen: Outcrossed: x0 Made by: Hilary Kemp Received: 03/15/25 University of Washington ----------------------------------------------------------------------------- Strain: DLM2 Species: Caenorhabditis elegans Genotype: uwaSi1 II; unc-119(ed3) III. Description: uwaSi1 [eft-3p::CERULEAN-VENUS::lgg-1 + unc-119(+)] II. MosSCI insertion into ttTi5605 II. Ubiquitously-expressed lgg-1 tagged with oxCerulean-oxVenus double fluorescent protein (dFP) for monitoring autophagy. Reference: Chapin H, et al. Aging (Albany NY). 2015 Jun;7(6):419-34. Mutagen: MosSCI Outcrossed: x0 Made by: Hannah Chapin Received: 08/21/15 University of Washington ----------------------------------------------------------------------------- Strain: DLM21 Species: Caenorhabditis elegans Genotype: unc-119(ed3)III; uwaEx4. Description: uwaEx4 [ubc-18::GFP::HA + myo-3p::RFP + unc-119(+)]. Pick RFP+ animals to maintain. Translational GFP transgene rescues ubc-18(tm5426). Mutagen: Outcrossed: x Made by: Hilary Kemp Received: 03/15/25 University of Washington ----------------------------------------------------------------------------- Strain: DLM22 Species: Caenorhabditis elegans Genotype: wdr-23(uwa5) I; hif-1(ia4) V. Description: Resistant to hydrogen sulfide. Mutagen: EMS Outcrossed: x4 Made by: Joseph Horsman Received: 06/01/17 University of Washington ----------------------------------------------------------------------------- Strain: DLM23 Species: Caenorhabditis elegans Genotype: skn-1(uwa6) IV; hif-1(ia4) V. Description: Resistant to hydrogen sulfide. Mutagen: EMS Outcrossed: x4 Made by: Joseph Horsman Received: 06/01/17 University of Washington ----------------------------------------------------------------------------- Strain: DLM24 Species: Caenorhabditis elegans Genotype: wdr-23(uwa15) I; hif-1(ia4) V. Description: Resistant to hydrogen sulfide. Mutagen: EMS Outcrossed: x4 Made by: Joseph Horsman Received: 06/01/17 University of Washington ----------------------------------------------------------------------------- Strain: DLM25 Species: C. elegans Genotype: hif-1(ia4) V; otIs197. Description: otIs197 [unc-14p::hif-1(P621A) + ttx-3p::RFP]. Non-degradable form of HIF-1 expressed from unc-14 promoter in hif-1 mutant background for tissue-specific rescuing experiments. otIs197 rescues lethality in hif-1 mutant animals exposed to 50ppm hydrogen sulfide. Reference: Topalidou I & and Miller DL. bioRxiv 174581; doi: https://doi.org/10.1101/174581. Mutagen: Outcrossed: x1 Made by: Irini Topalidou Received: 09/26/17 University of Washington ----------------------------------------------------------------------------- Strain: DLM26 Species: C. elegans Genotype: hif-1(ia4) V; otEx3165. Description: otEx3165 [unc-120p::hif-1(P621A) + ttx-3p::RFP]. Non-degradable form of HIF-1 expressed from muscle-specific promoter in hif-1 mutant background for tissue-specific rescuing experiments. otEx3165 does not rescue lethality in hif-1 mutant animals exposed to 50ppm hydrogen sulfide. Reference: Topalidou I & and Miller DL. bioRxiv 174581; doi: https://doi.org/10.1101/174581. Mutagen: Outcrossed: x0 Made by: Irini Topalidou Received: 09/26/17 University of Washington ----------------------------------------------------------------------------- Strain: DLM3 Species: Caenorhabditis elegans Genotype: ttTi5605 II; unc-119(ed3) III; uwaEx2. Description: uwaEx2 [vha-6p::CERULEAN-VENUS::lgg-1 + unc-119(+)]. Pick non-Unc to maintain. lgg-1 tagged with oxCerulean-oxVenus double fluorescent protein (dFP) for monitoring autophagy in the intestines. Reference: Chapin H, et al. Aging (Albany NY). 2015 Jun;7(6):419-34. Mutagen: Outcrossed: x0 Made by: Hannah Chapin Received: 08/21/15 University of Washington ----------------------------------------------------------------------------- Strain: DLM4 Species: Caenorhabditis elegans Genotype: uwaSi2 II; unc-119(ed3) III. Description: uwaSi2 [vha-6p::CERULEAN-VENUS::lgg-1 + unc-119(+)] II. MosSCI insertion into ttTi5605 II. lgg-1 tagged with oxCerulean-oxVenus double fluorescent protein (dFP) for monitoring autophagy in the intestines. Reference: Chapin H, et al. Aging (Albany NY). 2015 Jun;7(6):419-34. Mutagen: MosSCI Outcrossed: x0 Made by: Hannah Chapin Received: 08/21/15 University of Washington ----------------------------------------------------------------------------- Strain: DLM5 Species: Caenorhabditis elegans Genotype: ttTi5605 II; unc-119(ed3) III; uwaEx3. Description: uwaEx3 [dpy-7p::CERULEAN-VENUS::lgg-1 + unc-119(+)]. Pick non-Unc to maintain. lgg-1 tagged with oxCerulean-oxVenus double fluorescent protein (dFP) for monitoring autophagy in the hypodermis. Reference: Chapin H, et al. Aging (Albany NY). 2015 Jun;7(6):419-34. Mutagen: Outcrossed: x0 Made by: Hannah Chapin Received: 08/21/15 University of Washington ----------------------------------------------------------------------------- Strain: DLM6 Species: Caenorhabditis elegans Genotype: uwaSi3 II; unc-119(ed3) III. Description: uwaSi3 [dpy-7p::CERULEAN-VENUS::lgg-1 + unc-119(+)] II. MosSCI insertion into ttTi5605 II. lgg-1 tagged with oxCerulean-oxVenus double fluorescent protein (dFP) for monitoring autophagy in the hypodermis. Reference: Chapin H, et al. Aging (Albany NY). 2015 Jun;7(6):419-34. Mutagen: MosSCI Outcrossed: x0 Made by: Hannah Chapin Received: 08/21/15 University of Washington ----------------------------------------------------------------------------- Strain: DLM7 Species: Caenorhabditis elegans Genotype: ttTi5605 II; unc-119(ed3) III; uwaEx4. Description: uwaEx4 [myo-2p::CERULEAN-VENUS::lgg-1 + unc-119(+)]. Pick non-Unc to maintain. lgg-1 tagged with oxCerulean-oxVenus double fluorescent protein (dFP) for monitoring autophagy in pharyngeal muscle. Reference: Chapin H, et al. Aging (Albany NY). 2015 Jun;7(6):419-34. Mutagen: Outcrossed: x0 Made by: Hannah Chapin Received: 08/21/15 University of Washington ----------------------------------------------------------------------------- Strain: DLM8 Species: Caenorhabditis elegans Genotype: uwaSi4 II; unc-119(ed3) III. Description: uwaSi4 [myo-2p::CERULEAN-VENUS::lgg-1 + unc-119(+)] II. MosSCI insertion into ttTi5605 II. lgg-1 tagged with oxCerulean-oxVenus double fluorescent protein (dFP) for monitoring autophagy in pharyngeal muscle. Reference: Chapin H, et al. Aging (Albany NY). 2015 Jun;7(6):419-34. Mutagen: MosSCI Outcrossed: x0 Made by: Hannah Chapin Received: 08/21/15 University of Washington ----------------------------------------------------------------------------- Strain: DLM9 Species: Caenorhabditis elegans Genotype: ttTi5605 II; unc-119(ed3) III; uwaEx5. Description: uwaEx5 [myo-3p::CERULEAN-VENUS::lgg-1 + unc-119(+)]. Pick non-Unc to maintain. lgg-1 tagged with oxCerulean-oxVenus double fluorescent protein (dFP) for monitoring autophagy in body wall muscle. Reference: Chapin H, et al. Aging (Albany NY). 2015 Jun;7(6):419-34. Mutagen: Outcrossed: x0 Made by: Hannah Chapin Received: 08/21/15 University of Washington ----------------------------------------------------------------------------- Strain: DLW109 Species: C. elegans Genotype: wrdSi23 I; unc-104(knu973[unc-104::AID]) II. Description: wrdSi23 [eft-3p::TIR1:F2A:mTagBFP:tbb2 3' UTR:: loxP] I. wrdSi23 is inserted at ttTi4348 (I: -5.32 cM). Pan-somatic expression of TIR1 co-factor for AID, and expression of AID-tagged blue protein in somatic nuclei. Auxin-inducible degradation (AID) tag inserted at C-terminus of endogenous unc-104 locus by CRISPR/Cas9. Can be used for auxin-induced immobilization of worms for live imaging. References: Cahoon CK and Libuda DE. bioRxiv 2021.05.25.445686; doi: https://doi.org/10.1101/2021.05.25.445686. Ashley GE, et al. Genetics. 2021 Mar 31;217(3):iyab006. doi: 10.1093/genetics/iyab006. PMID: 33677541 Mutagen: Outcrossed: x3 Made by: Cori K. Cahoon Received: 06/16/21 University of Oregon, Eugene, OR ----------------------------------------------------------------------------- Strain: DLW110 Species: C. elegans Genotype: wrdSi23 I; unc-18(knu969[unc-18::AID]) X. Description: wrdSi23 [eft-3p::TIR1:F2A:mTagBFP:tbb2 3' UTR:: loxP] I. wrdSi23 is inserted at ttTi4348 (I: -5.32 cM). Pan-somatic expression of TIR1 co-factor for AID, and expression of AID-tagged blue protein in somatic nuclei. Auxin-inducible degradation (AID) tag inserted at C-terminus of endogenous unc-18 locus by CRISPR/Cas9. Can be used for auxin-induced immobilization of worms for live imaging. References: Cahoon CK and Libuda DE. bioRxiv 2021.05.25.445686; doi: https://doi.org/10.1101/2021.05.25.445686. Ashley GE, et al. Genetics. 2021 Mar 31;217(3):iyab006. doi: 10.1093/genetics/iyab006. PMID: 33677541 Mutagen: Outcrossed: x3 Made by: Cori K. Cahoon Received: 06/16/21 University of Oregon, Eugene, OR ----------------------------------------------------------------------------- Strain: DLW112 Species: C. elegans Genotype: reSi7 I; unc-104(knu973[unc-104::AID]) II. Description: reSi7 [rgef-1p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (I:-5.32). Neuronal-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in neuronal nuclei. Auxin-inducible degradation (AID) tag inserted at C-terminus of endogenous unc-104 locus by CRISPR/Cas9. Can be used for auxin-induced immobilization of worms for live imaging. Strain generated by crossing endogenously tagged unc-104::AID into DV3805. reSi7 is at -5.32 cM. References: Cahoon CK and Libuda DE. bioRxiv 2021.05.25.445686; doi: https://doi.org/10.1101/2021.05.25.445686. Ashley GE, et al. Genetics. 2021 Mar 31;217(3):iyab006. doi: 10.1093/genetics/iyab006. PMID: 33677541. NOTE: PCR detection of reSi7 insert using the published primers has been reported to be defective. These primers designed by Sherlyn Wijaya and Claire Richardson to detect ttTi4338 (LG I) also work for reIs7: ttTi4338 (LG I) wrdSi23-F: cttcaaagaaatcgccgac wrdSi23-FP: AACAACGAGACCTACGTCG wrdSi23-R: Ctctaagatgtcggccac (wt ~300bp, mutant ~650bp). Mutagen: Outcrossed: x3 Made by: Cori K. Cahoon Received: 06/16/21 University of Oregon, Eugene, OR ----------------------------------------------------------------------------- Strain: DLW114 Species: C. elegans Genotype: reSi7 I; unc-18(knu969[unc-18::AID]) X. Description: reSi7 [rgef-1p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (I:-5.32). Neuronal-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in neuronal nuclei. Auxin-inducible degradation (AID) tag inserted at C-terminus of endogenous unc-18 locus by CRISPR/Cas9. Can be used for auxin-induced immobilization of worms for live imaging. Strain generated by crossing endogenously tagged unc-104::AID into DV3805. reSi7 is at -5.32 cM. References: Cahoon CK and Libuda DE. bioRxiv 2021.05.25.445686; doi: https://doi.org/10.1101/2021.05.25.445686. Ashley GE, et al. Genetics. 2021 Mar 31;217(3):iyab006. doi: 10.1093/genetics/iyab006. PMID: 33677541. NOTE: PCR detection of reSi7 insert using the published primers has been reported to be defective. These primers designed by Sherlyn Wijaya and Claire Richardson to detect ttTi4338 (LG I) also work for reIs7: ttTi4338 (LG I) wrdSi23-F: cttcaaagaaatcgccgac wrdSi23-FP: AACAACGAGACCTACGTCG wrdSi23-R: Ctctaagatgtcggccac (wt ~300bp, mutant ~650bp). Mutagen: Outcrossed: x3 Made by: Cori K. Cahoon Received: 06/16/21 University of Oregon, Eugene, OR ----------------------------------------------------------------------------- Strain: DLW124 Species: C. elegans Genotype: wrdSi22 I; unc-52(knu968[AID::unc-52]) II. Description: wrdSi22 [eft-3p::TIR1:F2A:mTagBFP::tbb2 3' UTR::SEC[LoxP + let-858 term + sqt-1(d) + hs::Cre + hygR + unc-54 term + LoxP]] I. wrdSi22 is inserted at ttTi4348 (-5.32 cM). Pick Rollers to maintain animals retaining the SEC in the insertion. SEC can be removed by heat shock-induced excision according to the protocol in Dickinson et. al. Genetics 2015. Pan-somatic expression of TIR1 co-factor for AID, and expression of AID-tagged blue protein in somatic nuclei. Auxin-inducible degradation (AID) tag inserted at N-terminus of endogenous unc-52 locus by CRISPR/Cas9. References: Cahoon CK and Libuda DE. bioRxiv 2021.05.25.445686; doi: https://doi.org/10.1101/2021.05.25.445686. Ashley GE, et al. Genetics. 2021 Mar 31;217(3):iyab006. doi: 10.1093/genetics/iyab006. PMID: 33677541 Mutagen: Outcrossed: x3 Made by: Cori K. Cahoon Received: 06/16/21 University of Oregon, Eugene, OR ----------------------------------------------------------------------------- Strain: DLW14 Species: C. elegans Genotype: unc-5(lib1[myo-3p::GFP(-) + unc-119(+) + myo-2p::GFP(Mos1)]) IV; krIs14 V. Description: krIs14 [hsp-16.48p::MosTransposase + lin-15(+) + unc-122p::GFP] V. Recessive Unc. unc-5(lib1) is a CRISPR/Cas9 engineered mutant carrying the Intersister/Intrachromatid Repair Assay (ICR Assay) cassette inserted into the endogenous unc-5 locus. Briefly, ICR assay cassette includes two tandem GFP cassettes: the upstream using the myo-3 (body wall) promoter with a truncated GFP coding sequence, and the down-stream using the myo-2 (pharynx) promoter with GFP coding sequence interrupted by a Mos1 Drosophila transposon. Excision of Mos1 yields a single DSB, which if repaired by intersister or intrachromatid recombination, then will yield GFP+ progeny. The krIs14 insertion carrying heat-shock inducible Mos1 transposase is marked with coelomocyte GFP expression. Reference: Toraason E, et al. Current Biology 2021. https://doi.org/10.1016/j.cub.2021.03.008 Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Marissa Glover Received: 06/16/21 University of Oregon, Eugene, OR ----------------------------------------------------------------------------- Strain: DM1017 Species: Caenorhabditis elegans Genotype: unc-52(e3003e998) II; ccar-1(ra5) IV. Description: Suppressed Unc-52. This strain was isolated after EMS mutagenesis of CB998 and subjected to whole-genome sequencing (Flibotte et al., Genetics 185: 431 - 441 (2010). In addition to the unc-52 and sup-38 mutations, it is homozygous for 300 other mutations determined from sequence data. All mutations are annotated in WormBase. The e3003 and e998 mutations were present in the original CB998 strain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00036200 Mutagen: EMS Outcrossed: x0 Made by: Erin Gilchrist Received: 09/30/10 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: DM1151 Species: Caenorhabditis elegans Genotype: ccar-1(ra20) IV. Description: WT in appearance and movement. Some slight muscle disorganization and gonad disorganization. Slightly reduced brood size. Only suppresses the unc-52(e1421) allele. Mutagen: EMS Outcrossed: x Made by: Erin Gilchrist Received: 12/13/04 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM1152 Species: Caenorhabditis elegans Genotype: ccar-1(ra21) IV. Description: WT in appearance and movement. Some slight body wall muscle disorganization and gonad disorganization. Slightly reduced brood size. Only suppresses the unc-52(e1421) allele. Mutagen: EMS Outcrossed: x Made by: Erin Gilchrist Received: 12/13/04 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM1153 Species: Caenorhabditis elegans Genotype: ccar-1(ra14) IV. Description: WT in appearance and movement. Some slight body wall muscle disorganization. More severe gonad disorganization, and greatly reduced brood size. Suppresses the e444, e998, 669, e1012, e1421 and st196 alleles of unc-52. Mutagen: EMS Outcrossed: x Made by: Erin Gilchrist Received: 12/13/04 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM1154 Species: Caenorhabditis elegans Genotype: ccar-1(ra5) IV. Description: WT in appearance and movement. Some slight body wall muscle disorganization. More severe gonad disorganization, and greatly reduced brood size. Suppresses the e444, e998, 669, e1012, e1421 and st196 alleles of unc-52. Mutagen: EMS Outcrossed: x Made by: Erin Gilchrist Received: 12/13/04 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM1208 Species: Caenorhabditis elegans Genotype: unc-112(r367ra202) V. Description: Intragenic revertant (ra202) of original unc-112 mutation. WBPaper 00005804. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM1245 Species: Caenorhabditis elegans Genotype: unc-112(r367) V; Y102F5A.1(ra238) dim-1(ra204) X. Description: Y102F5A.1. Deletion extents determined by oligo array CGH. Deletion size: ~14kb. Deletion left flank: GGCAATCCTGGCCGAAGCTTTGAAACGCCCGAGTAAAGCCAAGAAGCGTC. Deletion right flank: GTTGTCTTTATCGAACCGCGTTGTTGAACTGTTGCATGAATCATGATTTC. This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. URL: http://www.celeganskoconsortium.omrf.org. Mutagen: EMS Outcrossed: x Made by: Mary Gilbert Received: 05/14/08 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: DM1602 Species: Caenorhabditis elegans Genotype: hsp-1(ra807) IV; unc-23(e25) V. Description: Superficially wild-type. Temperature-sensistive. Maintain at 15C. hsp-1(ra807) is a missense allele that replaces the conserved Ala379 residue to a Val residue in the ATPase domain of the HSP-1 protein and fully suppresses the bent-head phenotype of unc-23(e25). Animals are sterile or arrest development as larvae at when grown at 20-25C. Reference: Rahmani P, Rogalski T, Moerman DG. (2015) Worm. In press. Mutagen: EMS Outcrossed: x0 Made by: Poupak Rahmani Received: 04/17/15 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM2 Species: Caenorhabditis elegans Genotype: dim-1(ra102) X. Description: Body wall muscle is disorganized when viewed using polarized light. Hermaphrodites move well and are indistinguishable from WT under dissecting microscope. Mutagen: EMS Outcrossed: x Made by: Gilbert & Moerman Received: 11/03/03 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM2407 Species: Caenorhabditis elegans Genotype: hsp-1(ra807) IV; dpy-11(e224) V. Description: Dpy. hsp-1(ra807) is a missense allele that replaces the conserved Ala379 residue to a Val residue in the ATPase domain of the HSP-1 protein and fully suppresses the bent-head phenotype of unc-23(e25). Reference: Rahmani P, Rogalski T, Moerman DG. (2015) Worm. In press. Mutagen: Outcrossed: x0 Made by: Poupak Rahmani Received: 04/17/15 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM3004 Species: Caenorhabditis elegans Genotype: unc-112(r367) V; raDf4/+ X. Description: The unc-112(r367); raDf4/+ hermaphrodites move better than unc-112(r367); +/+ animals (but not as well as WT). raDf4 homozygotes arrest as L2 larvae. raDf4 deletes dim-1. Mutagen: UV/Psoralen Outcrossed: x Made by: Mary Gilbert Received: 11/03/03 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM3006 Species: Caenorhabditis elegans Genotype: unc-112(r367) V; raDf6/+ X. Description: The unc-112(r367); raDf6/+ hermaphrodites move better than unc-112(r367); +/+ animals (but not as well as WT). raDf6 homozygotes arrest during embryogenesis. raDf6 deletes dim-1. Mutagen: Formaldehyde Outcrossed: x Made by: Teresa Rogalski Received: 11/03/03 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM3007 Species: Caenorhabditis elegans Genotype: unc-112(r367) V; +/raDf7 X. Description: raDf7 suppresses the paralyzed phenotype of unc-112(r367) because the deficiency deletes the dim-1 gene. Reduction or loss of the dim-1 gene product suppresses the unc-112(r367) phenotype. In this strain: Animals homozygous for raDf7 arrest as L1 or L2 larvae; Animals without raDf7 are paralyzed as adults; Animals heterozygous for raDf7 move reasonably well. Mutagen: Formaldehyde Outcrossed: x Made by: Received: 07/13/99 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM3009 Species: Caenorhabditis elegans Genotype: unc-112(r367) V; raDf9/+ X. Description: The unc-112(r367); raDf9/+ hermaphrodites move better than unc-112(r367); +/+ animals (but not as well as WT). raDf9 homozygotes arrest as L1 or L2 larvae. raDf9 deletes dim-1. Mutagen: Formaldehyde Outcrossed: x Made by: Teresa Rogalski Received: 11/03/03 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM3010 Species: Caenorhabditis elegans Genotype: unc-112(r367) V; raDf10/+ X. Description: The unc-112(r367); raDf10/+ hermaphrodites move better than unc-112(r367); +/+ animals (but not as well as WT). raDf10 homozygotes arrest as L1 or L2 larvae. raDf10 deletes dim-1. Mutagen: Formaldehyde Outcrossed: x Made by: Teresa Rogalski Received: 05/07/04 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM3011 Species: Caenorhabditis elegans Genotype: unc-112(r367) V; raDf11/+ X. Description: The unc-112(r367); raDf11/+ hermaphrodites move better than unc-112(r367); +/+ animals (but not as well as WT). raDf11 homozygotes arrest as L1 or L2 larvae. raDf11 deletes dim-1. Mutagen: Formaldehyde Outcrossed: x Made by: Teresa Rogalski Received: 05/07/04 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM3012 Species: Caenorhabditis elegans Genotype: unc-112(r367) V; raDf12/+ X. Description: The unc-112(r367); raDf12/+ hermaphrodites move better than unc-112(r367); +/+ animals (but not as well as WT). raDf12 homozygotes arrest as L1 or L2 larvae. raDf12 deletes dim-1. Mutagen: Formaldehyde Outcrossed: x Made by: Teresa Rogalski Received: 05/07/04 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM3409 Species: Caenorhabditis elegans Genotype: mnDp33 (X;IV)/+ IV; spc-1(ra409) X. Description: Animals heterozygous for mnDp33 are WT and segregate WT and lethals. Animals which have lost mnDp33 arrest as two fold L1 larvae. Animals which are homozygous for mnDp33 are also larval lethal (L1-L2). Mutagen: EMS Outcrossed: x Made by: K Norman Received: 12/12/02 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM3414 Species: Caenorhabditis elegans Genotype: unc-4(e120) let-268(ra414)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, paralyzed Dpys and lethals. Mutagen: EMS Outcrossed: x3 Made by: Ken Norman Received: 03/01/01 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM4415 Species: Caenorhabditis elegans Genotype: unc-52(st196ra515) II. Description: WT phenotype. May still carry mut-4(st700). Mutagen: Revertant Outcrossed: x Made by: Jason Bush Received: 02/15/06 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM4417 Species: Caenorhabditis elegans Genotype: mut-4(st700) I; unc-52(st196ra517) II. Description: WT movement and body wall muscle. Mutagen: Revertant Outcrossed: x Made by: Jason Bush Received: 02/15/06 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM5113 Species: Caenorhabditis elegans Genotype: unc-112(r367) V; raEx11. Description: raEx11 [(pDM#208) unc-112(+) + rol-6(su1006)]. Maintain by picking Rollers. raEx11 rescues the r367 phenotype. Segregates Unc-112 and Rollers. Mutagen: Outcrossed: x Made by: Teresa Rogalski Received: 02/09/04 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM5115 Species: Caenorhabditis elegans Genotype: unc-112(st581) V; raEx16. Description: raEx16 [unc-112::GFP + rol-6(su1006)]. Rollers. Pick Rollers to maintain. raEx16 produces a fully functional GFP-tagged unc-112 protein that localizes to the dense bodies and M-line in muscle cells, and rescues the lethal phenotype of unc-112(st581) homozygous animals. Reference: Rogalski TM, et al. J Cell Biol. 2000 Jul 10;150(1):253-64. Mutagen: Outcrossed: x Made by: Teresa Rogalski Received: 10/01/14 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM5116 Species: Caenorhabditis elegans Genotype: unc-112(gk1)/unc-39(e257) V. Description: C47E8.7. Heterozygotes are WT and segregate WT, Pat unc-112 homozygotes and Unc homozygotes. Pick WT to maintain. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: Formaldehyde Outcrossed: x1 Made by: Moerman Lab Received: 03/06/00 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: DM5117 Species: Caenorhabditis elegans Genotype: unc-52(gk3) II. Description: ZC101.2a. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: Formaldehyde Outcrossed: x>1 Made by: Moerman Lab Received: 03/06/00 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: DM5130 Species: Caenorhabditis elegans Genotype: unc-23(e25) V; raEx20. Description: raEx20 [unc-23::GFP + rol-6(su1006) + pPD95.75(GFP)]. Rollers. Pick Rollers to maintain. raEx20 encodes a functional GFP::tagged UNC-23 protein. Reference: Rahmani P, Rogalski T, Moerman DG. (2015) Worm. In press. Mutagen: Outcrossed: x0 Made by: Poupak Rahmani Received: 04/17/15 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM612 Species: Caenorhabditis elegans Genotype: unc-2(ra612) X. Description: Mutagen: EMS Outcrossed: x>3 Made by: Received: 07/20/04 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7016 Species: Caenorhabditis elegans Genotype: raEx16. Description: raEx16 [unc-112::GFP + rol-6(su1006)]. Rollers. Pick Rollers to maintain. raEx16 produces a fully functional GFP-tagged unc-112 protein that localizes to the dense bodies and M-line in muscle cells, and will rescue the lethal phenotype of unc-112(st581) homozygous animals. Reference: Rogalski TM, et al. J Cell Biol. 2000 Jul 10;150(1):253-64. Mutagen: Outcrossed: x Made by: Teresa Rogalski Received: 10/01/14 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7020 Species: Caenorhabditis elegans Genotype: raEx20. Description: raEx20 [unc-23::GFP + rol-6(su1006) + pPD95.75(GFP)]. Rollers. Pick Rollers to maintain. raEx20 encodes a functional GFP::tagged UNC-23 protein. Reference: Rahmani P, Rogalski T, Moerman DG. (2015) Worm. In press. Mutagen: Outcrossed: x0 Made by: Poupak Rahmani Received: 04/17/15 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7059 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx59. Description: raEx59 [T05G5.1p::F22B5.10(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7062 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx62. Description: raEx62 [T05G5.1p::B0412.3(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7065 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx65. Description: raEx65 [T05G5.1p::C53B4.3(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7066 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx66. Description: raEx66 [T05G5.1p::F46F11.1(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7067 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx67. Description: raEx67 [T05G5.1p::B0024.10(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7068 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx68. Description: raEx68 [T05G5.1p::D2013.9(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7069 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx69. Description: raEx69 [T05G5.1p::T06A10.3(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7070 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx70. Description: raEx70 [T05G5.1p::ZK1236.7(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7071 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx71. Description: raEx71 [T05G5.1p::K07G5.1(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7072 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx72. Description: raEx72 [T05G5.1p::W06D4.1(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7073 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx73. Description: raEx73 [T05G5.1p::F47F2.1(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7074 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx74. Description: raEx74 [T05G5.1p::C52B11.2(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7085 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx85. Description: raEx85 [T05G5.1p::T01G9.6(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7086 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx86. Description: raEx86 [T05G5.1p::T10B11.6(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7088 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx88. Description: raEx88 [T05G5.1p::ZK353.7(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7091 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx91. Description: raEx91 [T05G5.1p::F42C5.9(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7092 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx92. Description: raEx92 [T05G5.1p::F52A8.4(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7093 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx93. Description: raEx93 [T05G5.1p::K07H8.1(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7106 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx106. Description: raEx106 [T05G5.1p::ZK593.1(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7107 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx107. Description: raEx107 [T05G5.1p::F25B3.1(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7108 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx108. Description: raEx108 [T05G5.1p::ZK563.4(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7109 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx109. Description: raEx109 [T05G5.1p::C53B4.7(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7110 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx110. Description: raEx110 [T05G5.1p::T09A5.5(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7111 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx111. Description: raEx111 [T05G5.1p::Y54E10BR.4(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. Y54E10BR.4 also known as nobh-1. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7112 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx112. Description: raEx112 [T05G5.1p::D1037.4(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7113 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx113. Description: raEx113 [T05G5.1p::W04C9.4(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7114 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx114. Description: raEx114 [T05G5.1p::R119.3(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7116 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx116. Description: raEx116 [T05G5.1p::D2024.5(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7117 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx117. Description: raEx117 [T05G5.1p::C17G1.7(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7118 Species: Caenorhabditis elegans Genotype: raEx118. Description: raEx118 [T05G5.1p::ZK637.3 ORF(cDNA)::GFP + rol-6(su1006)]. Wild-type background with extrachromosomal array carrying dominant rol-6 and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7129 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx129. Description: raEx129 [T05G5.1p::W09C5.1(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7130 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx130. Description: raEx130 [T05G5.1p::Y57A10A.16(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7131 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx131. Description: raEx131 [T05G5.1p::F42G9.9(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7132 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx132. Description: raEx132 [T05G5.1p::F02A9.4(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7133 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx133. Description: raEx133 [T05G5.1p::C47B2.2(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7134 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx134. Description: raEx134 [T05G5.1p::Y48G10A.3(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7135 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx135. Description: raEx135 [T05G5.1p::T01G9.2(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7136 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx136. Description: raEx136 [T05G5.1p::Y52B11A.9(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7138 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx138. Description: raEx138 [T05G5.1p::F25H2.4(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7139 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx139. Description: raEx139 [T05G5.1p::K04G2.9(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7140 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx140. Description: raEx140 [T05G5.1p::R10E4.9(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7141 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx141. Description: raEx141 [T05G5.1p::B0035.15(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7142 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx142. Description: raEx142 [T05G5.1p::C47E12.5(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7143 Species: Caenorhabditis elegans Genotype: raEx143. Description: raEx143 [T05G5.1p::M01E11.7 ORF(cDNA)::GFP + rol-6(su1006)]. Wild-type background with extrachromosomal array carrying dominant rol-6 and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7145 Species: Caenorhabditis elegans Genotype: raEx145. Description: raEx145 [T05G5.1p::Y71F9B.3 ORF(cDNA)::GFP + rol-6(su1006)]. Wild-type background with extrachromosomal array carrying dominant rol-6 and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7146 Species: Caenorhabditis elegans Genotype: raEx146. Description: raEx146 [T05G5.1p::C49H3.6 ORF(cDNA)::GFP + rol-6(su1006)]. Wild-type background with extrachromosomal array carrying dominant rol-6 and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7154 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx154. Description: raEx154 [T05G5.1p::K04B12.3(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7155 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx155. Description: raEx155 [T05G5.1p::K01G5.8(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7158 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx158. Description: raEx158 [T05G5.1p::C47D12.2(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7159 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx159. Description: raEx159 [T05G5.1p::T01H3.2(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7160 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx160. Description: raEx160 [T05G5.1p::Y43F8B.2(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7161 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx161. Description: raEx161 [T05G5.1p::T01C3.2(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7162 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx162. Description: raEx162 [T05G5.1p::F25H8.2(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7163 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx163. Description: raEx163 [T05G5.1p::F08B12.4(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7164 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx164. Description: raEx164 [T05G5.1p::F57C2.5(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7165 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx165. Description: raEx165 [T05G5.1p::K01H12.1(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7172 Species: Caenorhabditis elegans Genotype: raEx172. Description: raEx172 [T05G5.1p::F53E2.1 ORF(cDNA)::GFP + rol-6(su1006)]. Wild-type background with extrachromosomal array carrying dominant rol-6 and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7174 Species: Caenorhabditis elegans Genotype: raEx174. Description: raEx174 [T05G5.1p::T27A1.4 ORF(cDNA)::GFP + rol-6(su1006)]. Wild-type background with extrachromosomal array carrying dominant rol-6 and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7176 Species: Caenorhabditis elegans Genotype: raEx176. Description: raEx176 [T05G5.1p::Y39A1C.1 ORF(cDNA)::GFP + rol-6(su1006)]. Wild-type background with extrachromosomal array carrying dominant rol-6 and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7177 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx177. Description: raEx177 [T05G5.1p::T20D3.8(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7179 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx179. Description: raEx179 [T05G5.1p::F01G4.5(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7182 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx182. Description: raEx182 [T05G5.1p::F25B3.6(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7183 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx183. Description: raEx183 [T05G5.1p::T10C6.6(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7184 Species: Caenorhabditis elegans Genotype: raEx184. Description: raEx184 [T05G5.1p::F22D6.2 ORF(cDNA)::GFP + rol-6]. Wild-type background with extrachromosomal array carrying dominant rol-6 and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7186 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx186. Description: raEx186 [T05G5.1p::M01E5.4(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7188 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx188. Description: raEx188 [T05G5.1p::R07E5.7(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7189 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx189. Description: raEx189 [T05G5.1p::F54C8.7(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7190 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx190. Description: raEx190 [T05G5.1p::M05D6.5(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7191 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx191. Description: raEx191 [T05G5.1p::F02E9.1(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7192 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx192. Description: raEx192 [T05G5.1p::Y54G11A.11(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7194 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx194. Description: raEx194 [T05G5.1p::Y37D8A.10(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7198 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx198. Description: raEx198 [T05G5.1p::F25H2.12(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7199 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx199. Description: raEx199 [T05G5.1p::T20D3.6(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7200 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx200. Description: raEx200 [T05G5.1pZK265.6(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7201 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx201. Description: raEx201 [T05G5.1p::R07H5.3(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7202 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx202. Description: raEx202 [T05G5.1p::Y37D8A.1(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7203 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx203. Description: raEx203 [T05G5.1p::Y39B6A.33(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7204 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx204. Description: raEx204 [T05G5.1p::Y113G7B.17(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7205 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx205. Description: raEx205 [T05G5.1p::C05G5.1(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7206 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx206. Description: raEx206 [T05G5.1p::M79.3(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7207 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx207. Description: raEx207 [T05G5.1p::F15G9.1(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7208 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx208. Description: raEx208 [T05G5.1p::M02B1.3(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7209 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx209. Description: raEx209 [T05G5.1p::F45G2.4(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7210 Species: Caenorhabditis elegans Genotype: raEx210. Description: raEx210 [T05G5.1p::C32D5.9 ORF(cDNA)::GFP + rol-6]. Wild-type background with extrachromosomal array carrying dominant rol-6 and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7213 Species: Caenorhabditis elegans Genotype: raEx213. Description: raEx213 [T05G5.1p::T03G6.3 ORF(cDNA)::GFP + rol-6]. Wild-type background with extrachromosomal array carrying dominant rol-6 and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7215 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx215. Description: raEx215 [T05G5.1p::Y15E3A.4(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7217 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx217. Description: raEx217 [T05G5.1p::Y43F4B.5(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7218 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx218. Description: raEx218 [T05G5.1p::R151.10(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7219 Species: Caenorhabditis elegans Genotype: raEx219. Description: raEx219 [T05G5.1pW03F9.1 ORF(cDNA)::GFP + rol-6]. Wild-type background with extrachromosomal array carrying dominant rol-6 and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7221 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx221. Description: raEx221 [T05G5.1p::F25D7.1(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7222 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx222. Description: raEx222 [T05G5.1p::M18.3(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7223 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx223. Description: raEx223 [T05G5.1p::Y54E5A.5(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7226 Species: Caenorhabditis elegans Genotype: raEx226. Description: raEx226 [T05G5.1p::B0303.2 ORF(cDNA)::GFP + rol-6]. Wild-type background with extrachromosomal array carrying dominant rol-6 and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7228 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx228. Description: raEx228 [T05G5.1p::Y43E12A.2(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7229 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx229. Description: raEx229 [T05G5.1p::F13B12.1(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7230 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx230. Description: raEx230 [T05G5.1p::R04F11.5(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7231 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx231. Description: raEx231 [T05G5.1p::T10C6.5(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7232 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx322. Description: raEx322 [T05G5.1p::F52F12.3(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7233 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx233. Description: raEx233 [T05G5.1p::F21C3.1(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7234 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx342. Description: raEx342 [T05G5.1p::ZK637.2(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7235 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx235. Description: raEx235 [T05G5.1p::Y46G5A.12(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7237 Species: Caenorhabditis elegans Genotype: raEx237. Description: raEx237 [T05G5.1p::C08F8.1 ORF(cDNA)::GFP + rol-6]. Wild-type background with extrachromosomal array carrying dominant rol-6 and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7241 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx241. Description: raEx241 [T05G5.1p::T05G5.5(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7242 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx242. Description: raEx242 [T05G5.1p::ZK856.11(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7243 Species: Caenorhabditis elegans Genotype: raEx243. Description: raEx243 [T05G5.1p::C16C10.11 ORF(cDNA)::GFP + rol-6]. Wild-type background with extrachromosomal array carrying dominant rol-6 and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7245 Species: Caenorhabditis elegans Genotype: raEx245. Description: raEx245 [T05G5.1p::Y40B1B.7 ORF(cDNA)::GFP + rol-6]. Wild-type background with extrachromosomal array carrying dominant rol-6 and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7247 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx247. Description: raEx247 [T05G5.1p::ZK1321.2(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7248 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx248. Description: raEx248 [T05G5.1p::Y105E8A.3(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7249 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx249. Description: raEx249 [T05G5.1p::ZK856.9(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7250 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx250. Description: raEx250 [T05G5.1p::K07F5.15(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7251 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx251. Description: raEx251 [T05G5.1p::B0001.6(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7252 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx252. Description: raEx252 [T05G5.1p::C05D9.3(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7253 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx253. Description: raEx253 [T05G5.1p::F25H2.11(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7254 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx254. Description: raEx254 [T05G5.1p::F14B6.2(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7255 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx255. Description: raEx255 [T05G5.1p::F20H11.3(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7256 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx256. Description: raEx256 [T05G5.1p::F48E3.8(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7257 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx257. Description: raEx257 [T05G5.1p::C55A6.10(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7258 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx258. Description: raEx258 [T05G5.1p::C40H1.6(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7265 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx265. Description: raEx265 [T05G5.1p::F52H3.7(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7266 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx266. Description: raEx266 [T05G5.1p::M03F4.7(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7267 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx267. Description: raEx267 [T05G5.1p::F09F7.2(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7268 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx682. Description: raEx682 [T05G5.1p::H20J04.5(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7269 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx269. Description: raEx269 [T05G5.1p::Y67H2A.5(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7271 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx271. Description: raEx271 [T05G5.1p::K04D7.3(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7272 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx272. Description: raEx272 [T05G5.1p::T11G6.8(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7273 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx273. Description: raEx273 [T05G5.1p::Y17G7B.7(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7274 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx274. Description: raEx274 [T05G5.1p::Y66H1A.3(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7275 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx275. Description: raEx275 [T05G5.1p::Y106G6A.1(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7277 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx277. Description: raEx277 [T05G5.1p::M176.4(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7278 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx278. Description: raEx278 [T05G5.1p::Y56A3A.32(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7279 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx279. Description: raEx279 [T05G5.1p::C35E7.8(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7281 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx281. Description: raEx281 [T05G5.1p::K02C4.4(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7285 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx285. Description: raEx285 [T05G5.1p::C04F12.3(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7286 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx286. Description: raEx286 [T05G5.1p::F13D12.4(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7287 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx287. Description: raEx287 [T05G5.1p::K01G5.1(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7288 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx288. Description: raEx288 [T05G5.1p::D2030.3(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7290 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx290. Description: raEx290 [T05G5.1p::T22A3.4(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7292 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx292. Description: raEx292 [T05G5.1p::C28H8.12(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7293 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx293. Description: raEx293 [T05G5.1p::W08E3.3(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7294 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx294. Description: raEx294 [T05G5.1p::D1007.4(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7295 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx295. Description: raEx295 [T05G5.1p::F28H1.2(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7296 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx296. Description: raEx296 [T05G5.1p::D1007.14(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7298 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx298. Description: raEx298 [T05G5.1p::F20D1.10(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7299 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx299. Description: raEx299 [T05G5.1p::R07B7.3(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7300 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx300. Description: raEx300 [T05G5.1p::W05G11.6(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7301 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx301. Description: raEx301 [T05G5.1p::W04A8.4(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7302 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx302. Description: raEx302 [T05G5.1p::C25D7.8(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7303 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx303. Description: raEx303 [T05G5.1p::T01D1.2(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7304 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx304. Description: raEx304 [T05G5.1p::F33A8.3(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7305 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx305. Description: raEx305 [T05G5.1p::F21C10.10(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7306 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx306. Description: raEx306 [T05G5.1p::ZC97.1(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7307 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx307. Description: raEx307 [T05G5.1p::Y57G11C.3(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7308 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx308. Description: raEx308 [T05G5.1p::T05D4.1(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7309 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx309. Description: raEx309 [T05G5.1p::F32A7.3(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7310 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx310. Description: raEx310 [T05G5.1p::C29F5.1(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7311 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx311. Description: raEx311 [T05G5.1p::F25B5.7(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7312 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx312. Description: raEx312 [T05G5.1p::F37H8.5(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7313 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx313. Description: raEx313 [T05G5.1p::F38B7.1(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7314 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx314. Description: raEx314 [T05G5.1p::Y71H2AM.15(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7315 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx315. Description: raEx315 [T05G5.1p::T04C9.4(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7316 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx316. Description: raEx316 [T05G5.1p::T20B3.2(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7317 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx317. Description: raEx317 [T05G5.1p::W03C9.2(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7318 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx318. Description: raEx318 [T05G5.1p::F26A3.4(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7319 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx319. Description: raEx319 [T05G5.1p::C33H5.19(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7320 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx320. Description: raEx320 [T05G5.1p::F01F1.5(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7321 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx321. Description: raEx321 [T05G5.1p::T22A3.2(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7322 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx322. Description: raEx322 [T05G5.1p::Y5F2A.2(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7323 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx323. Description: raEx323 [T05G5.1p::R11G1.6(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7324 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx324. Description: raEx324 [T05G5.1p::F57B10.14(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7325 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx325. Description: raEx325 [T05G5.1p::Y73B6BL.21(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7330 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx330. Description: raEx330 [T05G5.1p::Y38F1A.9(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7331 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx331. Description: raEx331 [T05G5.1p::K10C3.6(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7332 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx332. Description: raEx332 [T05G5.1p::Y55F3C.3(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444.844. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7333 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx333. Description: raEx333 [T05G5.1p::M03A8.4(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7334 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx334. Description: raEx334 [T05G5.1p::F53F10.1(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7335 Species: Caenorhabditis elegans Genotype: pxl-1(ok1483) III; raEx335. Description: raEx335 [pxl-1a::GFP + rol-6(su1086)]. Rollers. Transgene rescues ok1483 L1 lethality. Pick adults to maintain. Reference: Warner A, et al. Mol Biol Cell. 2011 Jul 15;22(14):2551-63. Mutagen: Outcrossed: x1 Made by: Adam Werner Received: 10/16/12 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7336 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx336. Description: raEx336 [T05G5.1p::D2030.5(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7337 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx337. Description: raEx337 [T05G5.1p::T06D10.1(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7338 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx338. Description: raEx338 [T05G5.1p::C04G6.4(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7339 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx339. Description: raEx339 [T05G5.1p::D2092.4(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7340 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx340. Description: raEx340 [T05G5.1p::C04F12.8(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7341 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx341. Description: raEx341 [T05G5.1p::T26E3.2(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7342 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx342. Description: raEx342 [T05G5.1p::T10B10.4(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7343 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx343. Description: raEx343 [T05G5.1p::F42H10.3(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7344 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx344. Description: raEx344 [T05G5.1p::F55C10.1(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7350 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx350. Description: raEx350 [T05G5.1p::W03A5.7(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7351 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx351. Description: raEx351 [T05G5.1p::K01A2.3(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7352 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx352. Description: raEx352 [T05G5.1p::C26E6.2(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7353 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx353. Description: raEx353 [T05G5.1p::R151.4(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7355 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx355. Description: raEx355 [T05G5.1p::F44A2.5(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7356 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx356. Description: raEx356 [T05G5.1p::C05E4.3(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7360 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx360. Description: raEx360 [T05G5.1p::Y45G5AM.6(cDNA)::GFP + pha-1(+) + rol-6]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7361 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx361. Description: raEx361 [T05G5.1p::D2063.3(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7362 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx362. Description: raEx362 [T05G5.1p::R31.2(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7363 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx363. Description: raEx363 [T05G5.1p::T28B4.3(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7364 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx364. Description: raEx364 [T05G5.1p::C08D8.2(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7365 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx365. Description: raEx365 [T05G5.1p::C24A3.2(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7366 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx366. Description: raEx366 [T05G5.1p::ZC395.10(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7367 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx367. Description: raEx367 [T05G5.1p::D2063.1(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7368 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx368. Description: raEx368 [T05G5.1p::K06A4.3(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7369 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx369. Description: raEx369 [T05G5.1p::M02D8.1(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7370 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx370. Description: raEx370 [T05G5.1p::ZC504.5(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7371 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx371. Description: raEx371 [T05G5.1p::C40C9.5(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7372 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx372. Description: raEx372 [T05G5.1p::ZK892.1(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7373 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx373. Description: raEx373 [T05G5.1p::W05H7.4(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7374 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx374. Description: raEx374 [T05G5.1p::ZK54.1(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7375 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx375. Description: raEx375 [T05G5.1p::F46F6.2(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7376 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx376. Description: raEx376 [T05G5.1p::K08C7.6(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7377 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx377. Description: raEx377 [T05G5.1p::F56B6.4(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7378 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx378. Description: raEx378 [T05G5.1p::F44G4.4(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7379 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx379. Description: raEx379 [T05G5.1p::T12D8.8(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7381 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx381. Description: raEx381 [T05G5.1p::F29B9.8(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7384 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx384. Description: raEx384 [T05G5.1p::W06A7.2(cDNA):::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7385 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx385. Description: raEx385 [T05G5.1p::R102.5(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7386 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx386. Description: raEx386 [T05G5.1p::C29F5.7(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7387 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx387. Description: raEx387 [T05G5.1p::Y57G7A.10(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7388 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx388. Description: raEx388 [T05G5.1p::Y9C2UA.1(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7391 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx391. Description: raEx391 [T05G5.1p::Y48C3A.16(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7392 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx392. Description: raEx392 [T05G5.1p::T22C1.6(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7393 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx393. Description: raEx393 [T05G5.1p::ZK643.1(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7396 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx396. Description: raEx396 [T05G5.1p::ZK1098.1(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7397 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx397. Description: raEx397 [T05G5.1p::C48D5.2(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7398 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx398. Description: raEx398 [T05G5.1p::K08E3.5(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7399 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx399. Description: raEx399 [T05G5.1p::Y53G8AR.8(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7414 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx414. Description: raEx414 [T05G5.1p::pat-6(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7418 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx418. Description: raEx418 [T05G5.1p::F36F2.1(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7420 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx420. Description: raEx420 [T05G5.1p::W01A11.2(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7421 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx421. Description: raEx421 [T05G5.1p::ZK1127.10(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7427 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx427. Description: raEx427 [T05G5.1p::T15B12.1(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7430 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx430. Description: raEx430 [T05G5.1p::F22F7.7(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7431 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; raEx431. Description: raEx431 [T05G5.1p::ZK1128.5(cDNA)::GFP + pha-1(+) + rol-6(su1006)]. Temperature-sensitive pha-1 mutant rescued by extrachromosomal array carrying pha-1(+), dominant rol-6, and cDNA::GFP fusion driven by muscle promoter (T05G5.1). Grow at 25 degrees to maintain. At 15 degrees maintain by picking Rol-6 animals. WBPaper00038444. Mutagen: Outcrossed: x Made by: Moerman Lab Received: 10/26/11 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7438 Species: Caenorhabditis elegans Genotype: +/mT1 II; pxl-1(ok1483)/mT1 [dpy-10(e128)] III; raEx438. Description: raEx438 [pxl-1p::pxl-1a(cDNA)::GFP + rol-6(su1086)]. Rollers. Transgene does not rescue ok1483 L1 lethality. Transgenic (Rol) heterozygotes segregate rolling non-Dpy (heterozygotes carrying raEx438), non-rolling non-Dpy (heterozygotes without the array), arrested L1 (ok1483 homozygotes) and sterlie Dpy (mT1 homozygotes). Pick rolling non-Dpy and check for correct segregation of progeny to maintain. Reference: Warner A, et al. Mol Biol Cell. 2011 Jul 15;22(14):2551-63. Mutagen: Outcrossed: x1 Made by: Adam Werner Received: 10/16/12 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM7439 Species: Caenorhabditis elegans Genotype: raEx151. Description: raEx151 [cpna-1::YFP + rol-6(su1006)]. GFP inserted into WRM0617dC02 fosmid at 3' end of cpna-1 gene. WRM0617dC02 fosmid with YFP inserted into the F31D5.3/cpna-1 gene in frame at its 3' end by recombineering. YFP expression in body wall muscle cells at dense bodies and M-lines; weak expression in the pharynx. Pick Rollers to maintain. Reference: Warner A, et al. Mol Biol Cell. 2013 Mar;24(5):601-16. Mutagen: Outcrossed: x0 Made by: Rogalski & Warner Received: 06/07/13 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM8005 Species: Caenorhabditis elegans Genotype: raIs5. Description: raIs5 [myo-3p::GFP::myo-3 + rol-6(su1006)]. Rollers. raIs5 produces a fully functional GFP-tagged myo-3 protein that localizes to myofilaments in muscle cells. Derived by integrating stEx30 in DM5133. Reference: Meissner B, et al. PLoS Genet. 2009 Jun;5(6):e1000537. Mutagen: UV Outcrossed: x0 Made by: Teresa Rogalski Received: 10/01/14 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DM8008 Species: Caenorhabditis elegans Genotype: raIs8. Description: raIs8 [unc-112::GFP + rol-6(su1006)]. Rollers. raIs8 produces a fully functional GFP-tagged UNC-112 protein that localizes to the dense bodies and M-line in muscle cells. Derived by integrating raEx16 in DM7016. Mutagen: UV Outcrossed: x0 Made by: Teresa Rogalski Received: 10/01/14 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: DMS107 Species: C. elegans Genotype: dmaIs10. Description: dmaIs10 [asp-17p::GFP + unc-54p::mCherry]. Reference: Jiang W, et al. Elife. 2018 Apr 17:7:e35037. doi: 10.7554/eLife.35037. PMID: 29664006 Mutagen: integrated by UV Outcrossed: x3 Made by: Arthur Owen Received: 03/13/25 University of California, San Francisco, CA ----------------------------------------------------------------------------- Strain: DMS1946 Species: C. elegans Genotype: dmaEx617. Description: dmaEx617 [fshr-1p::fshr-1::GFP + unc-54p::mCherry]. Pick mCherry+ animals to maintain. Extrachromosomal fshr-1p::fshr-1::GFP translation reporter. Reference: Wang C, et al. Aging Cell. 2023 Jan;22(1):e13735. doi: 10.1111/acel.13735. PMID: 36415159. Mutagen: Outcrossed: x0 Made by: Dengke Ma Lab Received: 03/13/25 University of California, San Francisco, CA ----------------------------------------------------------------------------- Strain: DMS1983 Species: C. elegans Genotype: dmaEx620. Description: dmaEx620 [rpl-28p::fshr-1(ECD)::mCherry + unc-54p::GFP]. Pick GFP+ animals to maintain. Extrachromosomal mCherry‐tagged FSHR‐1 extracellular domain reporter. Reference: Wang C, et al. Aging Cell. 2023 Jan;22(1):e13735. doi: 10.1111/acel.13735. PMID: 36415159. Mutagen: Outcrossed: x0 Made by: Dengke Ma Lab Received: 03/13/25 University of California, San Francisco, CA ----------------------------------------------------------------------------- Strain: DMS2308 Species: C. elegans Genotype: dmaIs136. Description: dmaIs136 [flr-2p::flr-2::GFP + unc-54p::mCherry]. Integrated flr-2p::flr-2::GFP translation reporter. Reference: Wang C, et al. Aging Cell. 2023 Jan;22(1):e13735. doi: 10.1111/acel.13735. PMID: 36415159. Mutagen: UV Outcrossed: x3 Made by: Dengke Ma Lab Received: 03/13/25 University of California, San Francisco, CA ----------------------------------------------------------------------------- Strain: DMS303 Species: C. elegans Genotype: nIs590 V. Description: nIs590 [fat-7p::fat-7::GFP + lin15(+)] V.  Derived by X-ray integration of waEx15. The fat-7::fat-7::GFP translational reporter is activated by 15C or acdh-11 loss-of-function. See Ma et al., Cell. 2015 May 21; 161(5): 1152–1163. Mutagen: X-ray Outcrossed: x7 Made by: Dengke Ma Received: 06/01/17 University of California, San Francisco, CA ----------------------------------------------------------------------------- Strain: DMS441 Species: C. elegans Genotype: acdh-11(n5878) III; nIs590 V. Description: nIs590 [fat-7p::fat-7::GFP + lin15(+)] V.  The fat-7::fat-7::GFP translational reporter is activated constitutively by loss of acdh-11 function. acdh-11(n5878) is a 366 bp deletion. Reference: Ma et al., Cell. 2015 May 21; 161(5): 1152–1163. Mutagen: EMS Outcrossed: x2 Made by: Dengke Ma Received: 06/01/17 University of California, San Francisco, CA ----------------------------------------------------------------------------- Strain: DMS640 Species: C. elegans Genotype: nIs470 IV. Description: nIs470 [cysl-2p::GFP + myo-2p::mCherry] IV.  The cysl-2::GFP reporter is activated by HIF-1 in egl-9, rhy-1 or vhl-1 loss-of-function mutants. Mutagen: X-ray Outcrossed: x8 Made by: Dengke Ma Received: 06/01/17 University of California, San Francisco, CA ----------------------------------------------------------------------------- Strain: DMS734 Species: C. elegans Genotype: dmaIs21. Description: dmaIs21 [zip-10p::GFP + unc-54p::mCherry]. Integrated zip-10p::GFP transcriptional reporter. Reference: Jiang W, et al. Elife. 2018 Apr 17:7:e35037. doi: 10.7554/eLife.35037. PMID: 29664006. Mutagen: UV Outcrossed: x3 Made by: Dengke Ma Lab Received: 03/13/25 University of California, San Francisco, CA ----------------------------------------------------------------------------- Strain: DN20 Species: Caenorhabditis elegans Genotype: nhr-6(lg6001) III. Description: Homozygotes have defective spermatheca development causing low brood size, abnormally shaped eggs, and occasional Egl. nhr-6(lg6000) allele was originally generated by Marius Hoener and Tri Nguyen. Mutagen: TMP Outcrossed: x5 Made by: Chris Gissendanner Received: 08/28/09 The University of Louisiana-Monroe, Monroe LA, USA ----------------------------------------------------------------------------- Strain: DP13 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: For PCR STS mapping. Differs from RW7000 in two Tc1 sites. Caenorhabditis elegans wild isolate. DP subclone of RW7000. Mutagen: Outcrossed: x Made by: Pilgrim D Received: 11/26/91 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: DP132 Species: Caenorhabditis elegans Genotype: edIs6 IV. Description: edIs6 [unc-119::GFP + rol-6(su1006)] IV. Strong Roller phenotype. Hets are not Rollers (despite the presence of the supposedly dominant su1006 mutation in the array), so heterozygous males mate well. edIs6 is the integration of an array carrying pDP#MMUGF12 and pRF4. pDP#MMUGD12 ia an unc-119::GFP fusion that encodes 101 aa of UNC-119 and was made from the Fire lab vector pPD95.77. pRF4 is the rol-6(su1006) plasmid that gives a Rol phenotype. This strain allows the nervous system to be visualized by GFP fluorescence. GFP expression starts in the early embryo and continues through adulthood in most, if not all, of the nervous system. The expression of a similar lacZ fusion (but carrying a nuclear localizing signal) is described in Genetics 141: 977-988 1995. Mutagen: Gamma Rays Outcrossed: x1 Made by: Morris Maduro Received: 07/01/97 University of Alberta, Edmonton, Canada ----------------------------------------------------------------------------- Strain: DP246 Species: Caenorhabditis elegans Genotype: unc-45(st601)/sC1 [dpy-1(s2170)] III. Description: Made from LV15. st601 is a non-conditional two-fold arrest lethal. Mutagen: Outcrossed: x Made by: Dave Pilgrim Received: 07/24/00 University of Alberta, Edmonton, Canada ----------------------------------------------------------------------------- Strain: DP38 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; daf-?. Description: Unc. Contains an unlinked dauer constitutive mutation. See HT1593 for a stock with the Daf mutation removed. Mutagen: EMS Outcrossed: x3 Made by: Morris Maduro Received: 06/13/01 University of Chicago, IL ----------------------------------------------------------------------------- Strain: DP485 Species: Caenorhabditis tropicalis Genotype: Ctr-dpy-10(ed73) II. Description: Homozygotes for this mutation express a dumpy phenotype, while heterozygotes are roller and slightly shorter-than-wildtype in length. This Ctr-dpy-10 mutation will serve as a suitable syntenic marker for chromosome II mutations in C. tropicalis that does not impact the viability or fecundity of the organism. Generated in a C. tropicalis JU1373 background. Hermaphrodite. Culture at 20°C or above. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Montana Bobinski Received: 07/18/23 University of Alberta, Edmonton, Canada ----------------------------------------------------------------------------- Strain: DPB2301 Species: Caenorhabditis elegans Genotype: mir-43(sjm1) II. Description: Homozygotes lack obvious gross phenotypes; miR-43(sjm1) accumulates in L4 larvae compared to wild-type miR-43. mir-43(sjm1) has an inversion of the miR-43 seed sequence. Reference: Stubna MW, et al. bioRxiv doi: 10.1101/2024.06.28/601170. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Michael Stubna Received: 07/12/24 Whitehead Institute, Cambridge, MA ----------------------------------------------------------------------------- Strain: DPB2312 Species: Caenorhabditis elegans Genotype: mir-43(sjm2) II. Description: Homozygotes lack obvious gross phenotypes. mir-43(sjm2) has positions 9-23 of miR-43 substituted for random sequence. This strain is also homozygous for a G>T point substitution at position 8 of miR-42. Reference: Stubna MW, et al. bioRxiv doi: 10.1101/2024.06.28/601170. Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Michael Stubna Received: 07/12/24 Whitehead Institute, Cambridge, MA ----------------------------------------------------------------------------- Strain: DPB2313 Species: Caenorhabditis elegans Genotype: mir-43(sjm3) II. Description: Homozygotes lack gross phenotypes. mir-43(sjm3) has positions 9-23 of miR-43 substituted for the 3' region of miR-82. This strain is also homozygous for a G>T point substitution at position 8 of miR-42, and furthermore has 22bp of sequence deleted between miR-42* and miR-42. Reference: Stubna MW, et al. bioRxiv doi: 10.1101/2024.06.28/601170. Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Michael Stubna Received: 07/12/24 Whitehead Institute, Cambridge, MA ----------------------------------------------------------------------------- Strain: DPB2314 Species: Caenorhabditis elegans Genotype: mir-43(sjm1) II; ebax-1(tm2321) IV. Description: Homozygotes lack obvious gross phenotypes, though some miRNAs are elevated due to a loss-of-function mutation in ebax-1. mir-43(sjm1) is an inversion of the seed sequence of miR-43. Generated by mating parental strain CZ9907 hermaphrodites to mir-43(sjm1) males. Reference: Stubna MW, et al. bioRxiv doi: 10.1101/2024.06.28/601170. Mutagen: Outcrossed: x0 Made by: Michael Stubna Received: 07/12/24 Whitehead Institute, Cambridge, MA ----------------------------------------------------------------------------- Strain: DPB2315 Species: Caenorhabditis elegans Genotype: mir-43(sjm2) II; ebax-1(tm2321) IV. Description: Homozygotes lack gross phenotypes, though some miRNAs are elevated due to loss-of-function mutation in ebax-1. mir-43(sjm2) has positions 9-23 of miR-43 substituted for random sequence. This strain also has a G>T point substitution at position 8 of miR-42. Generated by mating parental strain CZ9907 hermaphrodites to mir-43(sjm2) males. Reference: Stubna MW, et al. bioRxiv doi: 10.1101/2024.06.28/601170. Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Michael Stubna Received: 07/12/24 Whitehead Institute, Cambridge, MA ----------------------------------------------------------------------------- Strain: DPB2316 Species: Caenorhabditis elegans Genotype: mir-43(sjm3) II; ebax-1(tm2321) IV. Description: Homozygotes lack obvious gross phenotypes, though some miRNAs are elevated due to loss-of-function mutation in ebax-1. mir-43(sjm3) has positions 9-23 of miR-43 substituted for the 3' region of miR-82. This strain is also homozygous for a G>T point substitution at position 8 of miR-42, and furthermore has 22bp of sequence deleted between mir-42* and mir-42. Generated by mating parental strain CZ9907 hermaphrodites to mir-43(sjm3) males. Reference: Stubna MW, et al. bioRxiv doi: 10.1101/2024.06.28/601170. Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Michael Stubna Received: 07/12/24 Whitehead Institute, Cambridge, MA ----------------------------------------------------------------------------- Strain: DPX5454 Species: C. elegans Genotype: orn-1(tm5454) III. Description: Reference: Bailly AP, et al. Cell Rep. 2019 Oct 1;29(1):212-224.e8. doi: 10.1016/j.celrep.2019.08.070. PMID: 31577950 Mutagen: Outcrossed: x5 Made by: Aymeric Bailly Received: 10/13/21 CNRS, Montpellier, France ----------------------------------------------------------------------------- Strain: DQ3188 Species: Caenorhabditis elegans Genotype: unc-11(vg103[unc-11::GFP]) I. Description: Superficially wild-type. Endogenously-tagged UNC-11::GFP is expressed in most, if not all, neurons, epithelial cells and coelomocytes, and should be visible in the nerve ring through a dissecting scope.. Mutagen: Outcrossed: x0 Made by: Maria E Gallegos Received: 06/03/21 Cal State University, East Bay ----------------------------------------------------------------------------- Strain: DQM1035 Species: C. elegans Genotype: bmdSi284 I. Description: bmdSi284 [loxN::rpl-28p::TIR1(F79G)::T2A::DHB::2xmKate2] I. bmdSi284 is a single copy CRISPR/Cas9 insertion that ubiquitously co-expresses the mutant version of TIR1 for improved auxin-inducible degradation via 5-Ph-IAA and a CDK activity sensor consisting of a fragment of human DNA helicase B (DHB) fused to two copies of mKate2. Reference: Martinez MAQ, et al. Biology Open. 2022 Dec 15;11(12):bio059668. doi: 10.1242/bio.059668. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Michael Martinez Received: 12/22/22 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM104 Species: C. elegans Genotype: bmdSi15 I. Description: bmdSi15 [loxN + eef-1A.1p::GFP(1-10)::unc-54 3′ UTR + let-858 terminator + myo-2p::mCherry::3xHA::tbb-2 3′ UTR + loxP] I. bmdSi15 is a CRISPR-based integration into the ttTi4348 site (I:-5.32). Somatic expression of sfGFP(1-10) driven by the eft-3 promoter. CRISPR/Cas9-mediated recombination was used to insert eef-1a.1p::GFP into the standard MosSCI insertion site ttTi4348. Reference: Reference: Costa DS, et al. Development. 2023 May 1;150(9):dev201570. doi: 10.1242/dev.201570. PMID: 37039075. Mutagen: CRISPR Outcrossed: x0 Made by: Dave Matus Received: 03/07/24 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM1041 Species: C. elegans Genotype: bmdSi282. Description: bmdSi282 [^loxN^rgef-1p::mKate2-STOP-STOP-VHH4GFP::DAMc1]. Wild-type growth and movement. NanoDam toolkit will allows identification of direct genomic targets of TFs as well as chromatin modifiers. In this system, Dam methylase is fused with a binding reagent, an anti-GFP nanobody (vhhGFP4). Thus, genome-wide profiling can be achieved by combining cell type-specific Dam::vhhGFP4 fusion constructs with GFP knock-in alleles. Mutagen: Outcrossed: x2 Made by: Taylor N. Medwig-Kinney Received: 03/21/23 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM1051 Species: C. elegans Genotype: lin-12(ljf31[lin-12::mNeonGreen[C1]::loxP::3xFLAG]) III; lag-2(bmd202[lag-2::P2A::H2B::mTurquoise2::lox511i::2xHA]) V. Description: Endogenously-tagger reporters allow simultaneous visualization of endogenous LIN-12 localization and lag-2 expression levels. Reference: Medwig-Kinney TN, et al. An in vivo toolkit to visualize endogenous LAG-2/Delta and LIN-12/Notch signaling in C. elegans. MicroPubl Biol. 2022 Jul 28;2022:10.17912/micropub.biology.000602. doi: 10.17912/micropub.biology.000602. PMID: 35966395. Mutagen: Outcrossed: x0 Made by: Taylor Medwig-Kinney and Ariel Pani Received: 10/23/23 University of Virginia ----------------------------------------------------------------------------- Strain: DQM1059 Species: C. elegans Genotype: bmdSi245 I; hda-1(bmd134[HDA-1::GFP::loxP]) V. Description: bmdSi245 [^SEC^lin-29p::mKate2-STOP-STOP-DAMc1::VHH4GFP] I. Relatively slow growth compared to N2 animals. NanoDam toolkit will allows identification of direct genomic targets of TFs as well as chromatin modifiers. In this system, Dam methylase is fused with a binding reagent, an anti-GFP nanobody (vhhGFP4). Thus, genome-wide profiling can be achieved by combining cell type-specific Dam::vhhGFP4 fusion constructs with GFP knock-in alleles. Mutagen: Outcrossed: x2 Made by: Yutong Xiao Received: 03/21/23 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM1066 Species: C. elegans Genotype: cshIs128 II; lin-12(ljf33[lin-12::mNeonGreen[C1]::loxP::3xFLAG::AID*]) III; lag-2(bmd204[lag-2::mTurquoise2::lox511i::2xHA]) V. Description: cshIs128 [rpl-28p::TIR1::T2A::mCherry::HIS-11)] II. Endogenously tagged LIN-12::mNG::3xFlag::AID crossed to endogenously tagged LAG-2::mTurquoise2::2xHA and ubiquitously expressed TIR1 with nuclear mCherry marker. Reference: Medwig-Kinney TN, et al. An in vivo toolkit to visualize endogenous LAG-2/Delta and LIN-12/Notch signaling in C. elegans. MicroPubl Biol. 2022 Jul 28;2022:10.17912/micropub.biology.000602. doi: 10.17912/micropub.biology.000602. PMID: 35966395. Mutagen: Outcrossed: x0 Made by: Taylor Medwig-Kinney and Theresa Gibney Received: 10/23/23 University of Virginia ----------------------------------------------------------------------------- Strain: DQM1068 Species: C. elegans Genotype: cshIs140 II; lin-12(ljf33[lin-12::mNeonGreen[C1]::loxP::3xFLAG::AID*]) III; lag-2(bmd204[lag-2::mTurquoise2::lox511i::2xHA]) V. Description: cshIs140 [rpl-28p::TIR1(F79G)::T2A::mCherry::HIS-11] II. Endogenously tagged LIN-12::mNG::3xFlag::AID crossed to endogenously tagged LAG-2::mTurquoise2::2xHA and ubiquitously expressed TIR1(F79G) with a nuclear mCherry marker. Reference: Medwig-Kinney TN, et al. An in vivo toolkit to visualize endogenous LAG-2/Delta and LIN-12/Notch signaling in C. elegans. MicroPubl Biol. 2022 Jul 28;2022:10.17912/micropub.biology.000602. doi: 10.17912/micropub.biology.000602. PMID: 35966395. Mutagen: Outcrossed: x0 Made by: Taylor Medwig-Kinney and Theresa Gibney Received: 10/23/23 University of Virginia ----------------------------------------------------------------------------- Strain: DQM1070 Species: C. elegans Genotype: cshIs128 II; lin-12(ljf33[lin-12::mNeonGreen[C1]::LoxP::3xFLAG::AID]) III; lag-2(bmd202[lag-2::P2A::H2B::mTurquoise2::lox511i::2xHA]) V. Description: cshIs128 [rpl-28p::TIR1::T2A::mCherry::his-11)] II. Auxin-dependent degradation of endogenous LIN-12 with visible readout of endogenous lag-2 expression. Reference: Pani AM, et al. A new toolkit to visualize and perturb endogenous LIN-12/Notch signaling in C. elegans. MicroPubl Biol. 2022 Jul 28;2022:10.17912/micropub.biology.000603. doi: 10.17912/micropub.biology.000603. PMID: 35966394. Mutagen: Outcrossed: x0 Made by: Theresa Gibney and Taylor Medwig-Kinney Received: 10/23/23 University of Virginia ----------------------------------------------------------------------------- Strain: DQM1072 Species: C. elegans Genotype: cshIs140 II; lin-12(ljf33[lin-12::mNeonGreen[C1]::loxP::3xFLAG::AID*]) III; lag-2(bmd202[lag-2::P2A::H2B::mTurquoise2::lox511i::2xHA]) V. Description: cshIs140 [rpl-28p::TIR1(F79G)::T2A::mCherry::HIS-11] II. Allows for conditional degradation of endogenous LIN-12 using 5-Ph-IAA. Reference: Pani AM, et al. A new toolkit to visualize and perturb endogenous LIN-12/Notch signaling in C. elegans. MicroPubl Biol. 2022 Jul 28;2022:10.17912/micropub.biology.000603. doi: 10.17912/micropub.biology.000603. PMID: 35966394. Mutagen: Outcrossed: x0 Made by: Theresa Gibney and Taylor Medwig-Kinney Received: 10/23/23 University of Virginia ----------------------------------------------------------------------------- Strain: DQM1113 Species: C. elegans Genotype: bmdSi297 II. Description: bmdSi297 [loxN::rpl-28p::FRT3::STOP::FRT3::TIR1(F79G)::T2A::DHB::2xmKate2]; inserted into safe harbor site ttTi5605 in Chr II. Ubiquitous rpl-28 promoter driving expression of FRT3::STOP::FRT3::TIR1(F79G)::DHB construct dependent upon tissue-specific FLPase. High levels of TIR1(F79G) can be expressed in specific tissue or cell types via FLPase activity, allowing spatiotemporally-targeted degradation of AID-tagged proteins. Reference: Xiao Y, et al. An expandable FLP-ON::TIR1 system for precise spatiotemporal protein degradation in C. elegans. bioRxiv 2022.10.14.512315; doi: https://doi.org/10.1101/2022.10.14.512315 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Matus Lab Received: 10/24/22 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM1118 Species: C. elegans Genotype: icbSi228 II; unc-119(ed3) III; ama-1(ers49[ama-1::degron::gfp]) IV. Description: icbSi228 [ttTi5605_right::wrt-2p::wCherry::Dam:linker:egl-13NLS::vhhGFP4::unc-54::unc-119 3'UTR::unc-119::unc-119p::ttTi5605_left)] II. Wild-type growth and movement. Mutagen: Outcrossed: x2 Made by: Yutong Xiao Received: 03/21/23 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM1126 Species: C. elegans Genotype: icbSi228 II; unc-119(ed3) III; had-1(bmd134[had-1::GFP::loxP]) V. Description: icbSi228 [ttTi5605_right::wrt-2p::wcherry::Dam:linker:egl-13NLS:vhhGFP4::unc-54::unc1193'UTR::unc-119::unc-119p::ttTi5605_left)] II. Wild-type growth and movement. Mutagen: Outcrossed: x2 Made by: Yutong Xiao Received: 03/21/23 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM1138 Species: C. elegans Genotype: dpff-1(bmd302[dpff-1p::^SEC^mNG::AID::DPFF-1]) III. Description: Pick Rollers to maintain. Endogenous N-term tagged DPFF-1 with mNG::AID using the self-excising cassette for drug selection. Animals are rollers which contains sqt-1 gene. Remove the SEC for normal expression using the protocol described in "Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B. Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette. Genetics. 2015 Aug;200(4):1035-49. doi: 10.1534/genetics.115.178335. Epub 2015 Jun 3. PMID: 26044593; PMCID: PMC4574250." Mutagen: Outcrossed: x0 Made by: Yutong Xiao Received: 03/21/23 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM1152 Species: C. elegans Genotype: bmdSi243 I; ljf3(unc-34::mNG[C1]^3xFlag::AID) V; qy41(lam-2::mKate2) X. Description: bmdSi243 (LoxN + cdh-3p::TIR1::F2A::DHB::2xmTurquoise2) I. bmdSi243 is a MosSCI insertion. mNG tag inserted into the C-temrinus of the endogenous unc-34 locus. mKate2 tag inserted into the C-temrinus of the endogenous lam-2 locus. Mutagen: Crispr/Cas9 Outcrossed: x Made by: David Matus Received: 04/18/23 Duke University ----------------------------------------------------------------------------- Strain: DQM1244 Species: C.elegans Genotype: bmdSi327 I. Description: bmdSi327 [loxN::ckb-3p::FLP::P2A::H2B::2xmTurq2]; inserted into safe harbor site ttTi4348 in Chr I. Uterine-specific expression of FLPase in Z1/Z4 and their descendants with blue fluorescent histone reporter for visualization. Reference: Xiao Y, et al. An expandable FLP-ON::TIR1 system for precise spatiotemporal protein degradation in C. elegans. bioRxiv 2022.10.14.512315; doi: https://doi.org/10.1101/2022.10.14.512315 Mutagen: Outcrossed: x2 Made by: Matus Lab Received: 10/24/22 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM1256 Species: C. elegans Genotype: bmdSi346 I; bmdSi297 II. Description: bmdSi346 [loxN::lin-31p::FLP]; inserted into safe harbor site ttTi4348 in Chr I. bmdSi297 [loxN::rpl-28p::FRT3::STOP::FRT3::TIR1(F79G)::T2A::DHB::2xmKate2]; inserted into safe harbor site ttTi5605 in Chr II. FLP-ON::TIR1 system for AID-tagged protein degradation in VPCs. High levels of TIR1(F79G) expression in vulval precursor cells by lin-31p::FLP with co-expression of CDK activity sensor. bmdSi297 contains the ubiquitous rpl-28 promoter driving expression of FRT3::STOP::FRT3::TIR1(F79G)::DHB construct dependent upon tissue-specific FLPase. High levels of TIR1(F79G) can be expressed in specific tissue or cell types via FLPase activity, allowing spatiotemporally-targeted degradation of AID-tagged proteins. Reference: Xiao Y, et al. An expandable FLP-ON::TIR1 system for precise spatiotemporal protein degradation in C. elegans. bioRxiv 2022.10.14.512315; doi: https://doi.org/10.1101/2022.10.14.512315 Mutagen: Outcrossed: x2 Made by: Matus Lab Received: 10/24/22 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM1258 Species: C. elegans Genotype: bmdSi348 I. Description: bmdSi348 [loxN::rgef-1p::FLP::P2A::H2B::2xmTurq2]; inserted into safe harbor site ttTi4348 in Chr I. Pan-neuronal expression of FLPase with blue fluorescent histone reporter for visualization. Reference: Xiao Y, et al. An expandable FLP-ON::TIR1 system for precise spatiotemporal protein degradation in C. elegans. bioRxiv 2022.10.14.512315; doi: https://doi.org/10.1101/2022.10.14.512315 Mutagen: Outcrossed: x2 Made by: Matus Lab Received: 10/24/22 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM1260 Species: C. elegans Genotype: bmdSi350 I. Description: bmdSi350 [loxN::wrt-2p::FLP::P2A::H2B::2xmTurq2]; inserted into safe harbor site ttTi4348 in Chr I. Hypodermal (seam cell) expression of FLPase with blue fluorescent histone reporter for visualization. Reference: Xiao Y, et al. An expandable FLP-ON::TIR1 system for precise spatiotemporal protein degradation in C. elegans. bioRxiv 2022.10.14.512315; doi: https://doi.org/10.1101/2022.10.14.512315 Mutagen: Outcrossed: x2 Made by: Matus Lab Received: 10/24/22 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM1261 Species: C. elegans Genotype: bmdSi338 I; qyIs225 V; lam-2(qy20[lam-2::mNG]) X. Description: bmdSi338 [^SEC^lin-29p::FLP::p2A::H2B::2xmTurq2] I. qyIs225 [cdh-3p::mCherry::moeABD] V. Pick Rollers to maintain. Wild-type growth and movement. mNG tag inserted into endogenous lam-2 locus. Anchor cell-specific FLP for targeted protein degradation. Reference: Xiao Y, et al. Genetics. 2023 PMID: 36722258. Mutagen: Outcrossed: x2 Made by: Yutong Xiao Received: 03/21/23 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM1283 Species: C. elegans Genotype: bmdSi348 I; bmdSi362 II. Description: bmdSi348 [loxN::rgef-1p::FLP::P2A::H2B::2xmTurq2]; inserted into safe harbor site ttTi4348 in Chr I. bmdSi362 [loxN::rpl-28p::FRT3-LCK::mNG-STOP::FRT3::TIR1(F79G)::2A::PH::2xmKate2]; inserted into safe harbor site ttTi5605 in Chr II. FLP-ON::TIR1 system for AID-tagged protein degradation in neurons. High levels of TIR1(F79G) expression in neurons by rgef-1p::FLP with co-expression of membrane markers. bmdSi362 contains the ubiquitous rpl-28 promoter driving expression of FRT3-LCK::mNG-STOP::FRT3::TIR1(F79G)::2A::PH::2xmKate2 construct dependent upon tissue-specific FLPase. High levels of TIR1(F79G) can be expressed in specific tissue or cell types via FLPase activity, allowing spatiotemporally-targeted degradation of AID-tagged proteins. Reference: Xiao Y, et al. An expandable FLP-ON::TIR1 system for precise spatiotemporal protein degradation in C. elegans. bioRxiv 2022.10.14.512315; doi: https://doi.org/10.1101/2022.10.14.512315 Mutagen: Outcrossed: x2 Made by: Matus Lab Received: 10/24/22 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM1285 Species: C. elegans Genotype: bmdSi363 I; egl-43(bmd88[egl-43p::egl-43::LoxP::GFP::egl-43]) II. Description: bmdSi363 [^SEC^ser-2p::mKate2-STOP-STOP-DAMc1::VHH4GFP] I. Pick Rollers to maintain. Wild-type growth. NanoDam toolkit will allows identification of direct genomic targets of TFs as well as chromatin modifiers. In this system, Dam methylase is fused with a binding reagent, an anti-GFP nanobody (vhhGFP4). Thus, genome-wide profiling can be achieved by combining cell type-specific Dam::vhhGFP4 fusion constructs with GFP knock-in alleles. Mutagen: Outcrossed: x2 Made by: Yutong Xiao Received: 03/21/23 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM298 Species: C.elegans Genotype: bmdSi86 I. Description: bmdSi86 [LoxN::rps-27p::DHB::GFP::P2A::H2B::mKate2] I. bmdSi86 is a single-copy CRISPR/Cas9-engineered insertion of a codon-optimized CDK sensor (amino acids 994–1087 of human DNA Helicase B (DHB) fused to GFP) co-expressed with his-58 (H2B) fused to two copies of mKate2. Reference: Adikes RC, et al. "Visualizing the metazoan proliferation-terminal differentiation decision in vivo." bioRxiv 2019.12.18.881888 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Simeiyun Liu Received: 12/18/20 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM300 Species: C. elegans Genotype: egl-43(bmd88[LoxP::gfp::egl-43]) II. Description: GFP reporter inserted internally into endogenous egl-43 locus. Reference: Medwig-Kinney TN, et al. Development. 2020 Jan 2;147(1). Mutagen: Outcrossed: x0 Made by: Taylor N. Medwig-Kinney Received: 02/28/20 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM311 Species: C. elegans Genotype: hlh-2(bmd90[LoxP::GFP::hlh-2]) I. Description: GFP reporter inserted into N-terminus of endogenous hlh-2 locus. Reference: Medwig-Kinney TN, et al. Development. 2020 Jan 2;147(1). Mutagen: Outcrossed: x0 Made by: Taylor N. Medwig-Kinney Received: 02/28/20 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM455 Species: C. elegans Genotype: cki-1(bmd132[GFP::LoxP::cki-1::3xFLAG]) II. Description: CRISPR/Cas9 insertion of GFP into the N-terminus of cki-1; seems to cause cki-1 gain-of-function phenotype (early cell cycle exit for some post-embryonic blast cells). Reference: Adikes RC, et al. "Visualizing the metazoan proliferation-terminal differentiation decision in vivo." bioRxiv 2019.12.18.881888 Mutagen: Crispr/Cas9 Outcrossed: x>4 Made by: Nicholas J. Palmisano Received: 12/18/20 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM494 Species: C. elegans Genotype: egl-43(bmd136[LoxP::gfp::egl-43(long)]) II. Description: GFP reporter inserted into N-terminus of endogenous egl-43 locus specifically tags the long isoform. Reference: Medwig-Kinney TN, et al. Development. 2020 Jan 2;147(1). Mutagen: Outcrossed: x0 Made by: Taylor N. Medwig-Kinney Received: 02/28/20 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM497 Species: C. elegans Genotype: fos-1(bmd138[LoxP::gfp::fos-1]) V. Description: GFP reporter inserted into N-terminus of endogenous fos-1 locus. Reference: Medwig-Kinney TN, et al. Development. 2020 Jan 2;147(1). Mutagen: Outcrossed: x0 Made by: Taylor N. Medwig-Kinney Received: 02/28/20 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM543 Species: C. elegans Genotype: bmdSi147 I. Description: bmdSi147 [loxN::rps-27p::DHB::2xmKate2::P2A::H2B::GFP] I. bmdSi147 is a single-copy CRISPR/Cas9-engineered insertion of a codon-optimized CDK sensor (amino acids 994–1087 of human DNA Helicase B (DHB) fused to two copies of mKate2) co-expressed with his-58 (H2B) fused to GFP. Reference: Adikes RC, et al. "Visualizing the metazoan proliferation-terminal differentiation decision in vivo." bioRxiv 2019.12.18.881888 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Taylor N. Medwig-Kinney Received: 12/18/20 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM583 Species: C. elegans Genotype: bmdSi141 I. Description: bmdSi141 [loxN::eft-3p::his-58::GFP] I. Slow growing. Maintain at 15-20C. bmdSi141 is a single-copy CRISPR/Cas9-engineered insertion of HIS-58 C-terminally tagged with codon-optimized GFP and driven by the ubiquitous eft-3 promoter. Reference: Azmi MA, et al. bioRxiv 2020.10.17.344069; doi: https://doi.org/10.1101/2020.10.17.344069 Mutagen: Outcrossed: x0 Made by: David Matus Received: 11/04/20 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM594 Species: C. elegans Genotype: bmdSi170 I. Description: bmdSi170 [loxN::eft-3p::his-58::GFP::3xHA] I. Superficially wild-type. bmdSi170 is a single-copy CRISPR/Cas9-engineered insertion of HIS-58 C-terminally tagged with non-codon-optimized GFP and driven by the ubiquitous eft-3 promoter. Reference: Azmi MA, et al. bioRxiv 2020.10.17.344069; doi: https://doi.org/10.1101/2020.10.17.344069 Mutagen: Outcrossed: x0 Made by: David Matus Received: 11/04/20 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM662 Species: C.elegans Genotype: bmdSi200 I; bmdSi168 II. Description: bmdSi200 [loxN::pcn-1p::pcn-1::GFP] I. bmdSi168 [loxN::rps-27p::DHB::2x-mKate2] II. bmdSi200 is a single copy CRISPR/Cas9-engineered insertion of a full length pcn-1::GFP translational fusion under its own promoter. bmdSi168 is a single-copy CRISPR/Cas9-engineered insertion of a codon optimized CDK sensor (amino acids 994–1087 of human DNA Helicase B (DHB) fused to two copies of mKate2). Reference: Adikes RC, et al. "Visualizing the metazoan proliferation-terminal differentiation decision in vivo." bioRxiv 2019.12.18.881888 Mutagen: Outcrossed: x0 Made by: Taylor N. Medwig-Kinney Received: 12/18/20 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM935 Species: C. elegans Genotype: bmdSi245 I; fos-1(bmd138[fos-1p::LoxP::GFP::FOS-1]) V. Description: bmdSi245 [^SEC^lin-29p::mKate2-STOP-STOP-DAMc1::VHH4GFP] I. Pick Rollers to maintain. Wild-type growth. NanoDam toolkit will allows identification of direct genomic targets of TFs as well as chromatin modifiers. In this system, Dam methylase is fused with a binding reagent, an anti-GFP nanobody (vhhGFP4). Thus, genome-wide profiling can be achieved by combining cell type-specific Dam::vhhGFP4 fusion constructs with GFP knock-in alleles. Mutagen: Outcrossed: x2 Made by: Yutong Xiao Received: 03/21/23 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM973 Species: C. elegans Genotype: bmdSi245 I; swsn-4(bmd63[LoxP::GFP::swsn-4]) IV. Description: bmdSi245 [^SEC^lin-29p::mKate2-STOP-STOP-DAMc1::VHH4GFP] I. Wild-type growth and movement. NanoDam toolkit will allows identification of direct genomic targets of TFs as well as chromatin modifiers. In this system, Dam methylase is fused with a binding reagent, an anti-GFP nanobody (vhhGFP4). Thus, genome-wide profiling can be achieved by combining cell type-specific Dam::vhhGFP4 fusion constructs with GFP knock-in alleles. Mutagen: Outcrossed: x2 Made by: Yutong Xiao Received: 03/21/23 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM979 Species: C. elegans Genotype: bmdSi245 swsn-8(bmd222[(swsn-8p::swsn-8::GFP]) I. Description: bmdSi245 [^SEC^lin-29p::mKate2-STOP-STOP-DAMc1::VHH4GFP] I. Wild-type growth and movement. NanoDam toolkit will allows identification of direct genomic targets of TFs as well as chromatin modifiers. In this system, Dam methylase is fused with a binding reagent, an anti-GFP nanobody (vhhGFP4). Thus, genome-wide profiling can be achieved by combining cell type-specific Dam::vhhGFP4 fusion constructs with GFP knock-in alleles. Mutagen: Outcrossed: x2 Made by: Yutong Xiao Received: 03/21/23 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DQM984 Species: C. elegans Genotype: bmdSi245 pbrm-1(st12226[pbrm-1::TY1::EGFP::3xFLAG]) I. Description: bmdSi245 [^SEC^lin-29p::mKate2-STOP-STOP-DAMc1::VHH4GFP] I. Wild-type growth and movement. NanoDam toolkit will allows identification of direct genomic targets of TFs as well as chromatin modifiers. In this system, Dam methylase is fused with a binding reagent, an anti-GFP nanobody (vhhGFP4). Thus, genome-wide profiling can be achieved by combining cell type-specific Dam::vhhGFP4 fusion constructs with GFP knock-in alleles. Mutagen: Outcrossed: x2 Made by: Yutong Xiao Received: 03/21/23 Stony Brook University, Stony Brook, NY ----------------------------------------------------------------------------- Strain: DR1 Species: Caenorhabditis elegans Genotype: unc-101(m1) I. Description: Paralyzed coiler. Slightly Egl. Slightly short. About half do not survive to adulthood-arrest usually in L1. See also WBPaper00001868. Mutagen: DES Outcrossed: x Made by: Riddle D Received: 01/01/76 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR101 Species: Caenorhabditis elegans Genotype: dpy-5(e61) unc-55(e1170) I. Description: Dpy. Unc. Closely Linked. Mutagen: Outcrossed: x Made by: Riddle D Received: 11/01/75 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR102 Species: Caenorhabditis elegans Genotype: dpy-5(e61) unc-29(e403) I. Description: DpyUnc. Mutagen: Outcrossed: x Made by: Riddle D Received: 11/01/75 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR103 Species: Caenorhabditis elegans Genotype: dpy-10(e128) unc-4(e120) II. Description: DpyUnc. [NOTE: Likely contains background mrt mutation (re47) (D. Reiner). Segregates males, sickly animals, and dark steriles growing progessively worse in subsequent generations. (D. Reiner & S. Ahmed, 2011)] Mutagen: Outcrossed: x Made by: Sulston J Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: DR104 Species: Caenorhabditis elegans Genotype: dpy-18(e364) unc-25(e156) dnj-17(ju1162) III. Description: DpyUnc. Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: DR105 Species: Caenorhabditis elegans Genotype: unc-17(e245) dpy-20(e1282) IV. Description: DpyUnc. Mutagen: Outcrossed: x Made by: Edgar B Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: DR1056 Species: Caenorhabditis elegans Genotype: unc-42(e270) ama-2(m323) V/nT1 [let-?(m435)] (IV;V). Description: Heterozygotes are WT and segregate WT and dead eggs. (Chromosome carrying unc-42 ama-2 is homozygous lethal.) Strain is resistant to _-amanitin. Mutagen: Outcrossed: x Made by: Rogalski T Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR106 Species: Caenorhabditis elegans Genotype: unc-24(e1172) dpy-4(e1166) IV. Description: DpyUnc. Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR107 Species: Caenorhabditis elegans Genotype: unc-26(e205) dpy-4(e1166) IV. Description: DpyUnc. Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR108 Species: Caenorhabditis elegans Genotype: dpy-11(e224) unc-42(e270) V. Description: DpyUnc. Mutagen: Outcrossed: x Made by: Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1089 Species: Caenorhabditis elegans Genotype: unc-77(e625) IV. Description: Dominant loopy Unc with exaggerated sine wave. Mutagen: Outcrossed: x Made by: Edgley M Received: 02/01/88 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1096 Species: Caenorhabditis elegans Genotype: lon-1(e185) unc-36(e251) III. Description: Very long, thin, and slow moving. Mutagen: Outcrossed: x Made by: Albert P Received: 03/14/88 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1099 Species: Caenorhabditis elegans Genotype: ama-1(m118m526) IV. Description: More resistant to alpha-amanitin than ama-1(m118). Will grow in the presence of 0.5% triton-X 100 with 100 ug/ml amanitin whereas ama-1(m118) will not. DR1099 displays temperature-sensitive defects consistent with defective RNA Pol II function. Sterile at 25C (maternal effect sterile). Fertile at 20C, but produces few viable progeny (~80% eggs that do not hatch). Periodically check for Emb to prevent spontaneously suppressed animals from taking over a population. Reference: Rogalski TM, et al. Genetics. 1990 Dec;126(4):889-98. Mutagen: EMS Outcrossed: x2 Made by: Rogalski/Riddle Received: 05/28/02 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR111 Species: Caenorhabditis elegans Genotype: him-4(e1266) unc-3(e151) X. Description: Unc. Segregates males. Mutagen: Outcrossed: x Made by: Riddle D Received: 12/01/75 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR112 Species: Caenorhabditis elegans Genotype: lon-2(e678) him-4(e1267) X. Description: Long. Segregates males. Mutagen: Outcrossed: x Made by: Riddle D Received: 12/01/75 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR113 Species: Caenorhabditis elegans Genotype: lon-2(e678) mec-4(e1339) X. Description: Long. Mechanosensory Abnormal. Sometimes touch sensitive in tail. Mutagen: Outcrossed: x Made by: Dew M Received: 12/01/75 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: DR115 Species: Caenorhabditis elegans Genotype: unc-15(e73) unc-54(e675) I. Description: Slow movement. Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: DR116 Species: Caenorhabditis elegans Genotype: unc-15(e73) I; sma-1(e30) V. Description: Small. Unc. Mutagen: Outcrossed: x Made by: Riddle D Received: 11/01/75 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR117 Species: Caenorhabditis elegans Genotype: unc-15(e73) I; dpy-11(e224) V. Description: Dpy. Unc. Very slow movement. Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1172 Species: Caenorhabditis elegans Genotype: let-60(s59) unc-22(s7) IV/nT1 [let-?(m435)] (IV;V). Description: Heterozygotes are WT and segregate WT, Lethal Twitchers, and dead eggs. Lethal mid-larval (leaky). Maintain by picking WT. Heterozygotes twitch in 1% nicotine. Mutagen: Outcrossed: x Made by: Edgley M Received: 04/01/89 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR118 Species: Caenorhabditis elegans Genotype: unc-15(e73) I; dpy-11(e224) eDp22 V. Description: Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR119 Species: Caenorhabditis elegans Genotype: unc-54(e1258) I; dpy-11(e224) wdDp1 V. Description: DPY. Mutagen: Outcrossed: x Made by: Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1191 Species: Caenorhabditis elegans Genotype: daf-12(m20) unc-27(e155) egl-15(n484) X. Description: Dauer defective. Egl-60% form bags of worms. Unc-sluggish, poor backing. Resistant to both imipramine and serotonin. Slightly Dpy. Mutagen: Outcrossed: x Made by: Yeh W-H Received: 06/08/89 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR120 Species: Caenorhabditis elegans Genotype: unc-54(e1258) I; eDp23 V. Description: Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1213 Species: Caenorhabditis elegans Genotype: unc-44(e362) unc-24(e138) IV. Description: Unc-44 masks the Unc-24 phenotype. Paralyzed coiler, Dpy, tends to curl. Mutagen: Outcrossed: x Made by: Albert P Received: 08/15/89 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1215 Species: Caenorhabditis elegans Genotype: unc-22(s7) let-67(s214) IV/nT1 [let-?(m435)] (IV;V). Description: Heterozygotes are WT and segregate WT, dead eggs and Twitchers that arrest as larvae. Maintain by picking WT. Hets twitch in 1% nicotine. Mutagen: Outcrossed: x Made by: Edgley M Received: 09/01/89 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1218 Species: Caenorhabditis elegans Genotype: mnC1 [dpy-10(e128) unc-52(e444)]/unc-4(e120) let-265(mn188) II. Description: Heterozygotes are WT and segregate WT, DpyUnc, and Lethal Unc-4 (lethal mid-larval). Maintain by picking WT. Mutagen: Outcrossed: x Made by: Edgley M Received: 11/09/89 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1228 Species: Caenorhabditis elegans Genotype: unc-45(e286) daf-7(e1372) dpy-1(e1) III. Description: Dpy. Temperature sensitive dauer constitutive (leaky). Temperature sensitive Unc. At 15C, Dpy adults and some dauers. At 25C, DpyUnc dauers and some DpyUnc adults (leakiness varies: 60-95% dauers). Mutagen: Outcrossed: x Made by: Albert P Received: 02/01/90 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR128 Species: Caenorhabditis elegans Genotype: dpy-1(e1) daf-2(e1370) III. Description: Temperature sensitive dauer constitutive. Dpy. 100% dauers at 25C. Mutagen: Outcrossed: x Made by: Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR129 Species: Caenorhabditis elegans Genotype: daf-2(e1370) unc-32(e189) III. Description: Temperature senstive dauer constitutive. Unc-coiler. 100% dauers at 25C. Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1290 Species: Caenorhabditis elegans Genotype: dpy-6(e14) unc-3(e151) X. Description: Medium sized Dpy which curls its tail. Mutagen: Outcrossed: x Made by: Estevez A Received: 10/24/90 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR130 Species: Caenorhabditis elegans Genotype: dpy-1(e1) daf-2(e1370) unc-32(e189) III. Description: Temperature sensitive dauer constitutive. 100% dauers at 25C. Dpy. Unc-coiler. Reference strain for daf-2 complementation. Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1309 Species: Caenorhabditis elegans Genotype: daf-16(m26) I; daf-2(e1370) III. Description: Mutagen: Outcrossed: x Made by: Patrice Albert Received: 12/01/97 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR133 Species: Caenorhabditis elegans Genotype: daf-7(e1372) dpy-1(e1) unc-32(e189) III. Description: Temperature sensitive dauer constitutive. 100% dauers at 25C. Dpy. Unc-coiler. Reference strain for daf-7 complementation. Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1344 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Reference WBG 10(2) 140-141. Previously called Bergerac LY and Bergerac BO. Caenorhabditis elegans wild isolate. DR subclone of Bergerac BO (Tc1 pattern HCA). Mutagen: Outcrossed: x Made by: Received: 01/01/80 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DR1345 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Reference WBG 10(2) 104-141 and 11(5) 60. Caenorhabditis elegans wild isolate. DR subclone of Cl2a (Tc1 pattern II). Mutagen: Outcrossed: x Made by: Received: 09/01/82 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DR1346 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Reference WBG 10(2) 140-141. Caenorhabditis elegans wild isolate. DR subclone of GA-1 (Tc1 pattern I). Mutagen: Outcrossed: x Made by: Johnson C Received: 09/01/82 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DR1347 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Reference WBG 10(2) 140-141. Caenorhabditis elegans wild isolate. DR subclone of GA-1 (Tc1 pattern I). Mutagen: Outcrossed: x Made by: Johnson C Received: 09/01/82 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DR1348 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Reference WBG 10(2) 140-141. Caenorhabditis elegans wild isolate. DR subclone of GA-1 (Tc1 pattern I). Mutagen: Outcrossed: x Made by: Johnson C Received: 09/01/82 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DR1349 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Reference WBG 10(2) 140-141 and 11(5) 60. Caenorhabditis elegans wild isolate. DR subclone of PA-1. Mutagen: Outcrossed: x Made by: Hedgecock E Received: 09/01/82 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DR1350 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Reference WBG 10(2) 140-141. Caenorhabditis elegans wild isolate. DR subclone of PA-2 (Tc1 pattern III). Mutagen: Outcrossed: x Made by: Hedgecock E Received: 09/01/82 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: DR1367 Species: Caenorhabditis elegans Genotype: bli-6(sc16) mec-3(e1338) IV. Description: Blistered. Mechanosensory abnormal. Mutagen: Outcrossed: x Made by: Ian Caldicott Received: 01/24/96 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1369 Species: Caenorhabditis elegans Genotype: sma-4(e729) III. Description: Short, non-Dpy. Males have crumpled spicules. Mutagen: EMS Outcrossed: x2 Made by: Riddle D Received: 10/16/91 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR137 Species: Caenorhabditis elegans Genotype: dpy-5(e61) daf-8(e1393) I. Description: Temperature sensitive dauer constitutive. Dpy. Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR138 Species: Caenorhabditis elegans Genotype: che-3(e1378) I; dpy-13(e184) IV. Description: Dauer defective. Dpy. Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR139 Species: Caenorhabditis elegans Genotype: che-3(e1379) I; dpy-13(e184) IV. Description: Dauer defective. Dpy. Mutagen: Outcrossed: x Made by: Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1407 Species: Caenorhabditis elegans Genotype: daf-12(m583) X. Description: daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele. Mutagen: EMS Outcrossed: x Made by: Don Riddle Received: 10/23/00 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: DR1408 Species: Caenorhabditis elegans Genotype: daf-16(m26) I; age-1(m333) II. Description: Egl phenotype, viable. Lethality and Daf-c phenotype of m333 suppressed by daf-16 (m333 alone is a maternal-effect non-conditional dauer constitutive). age-1(m333) pka daf-23(m333). Mutagen: Outcrossed: x2 Made by: Patrice Albert Received: 01/06/97 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1410 Species: Caenorhabditis elegans Genotype: dpy-27(y56)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: WT strain which segregates WT, Dpy and DpySteriles (or WT Steriles if grown at 15C.) Maintain by picking WT. dpy-27 animals are mildly Dpy and Egl with protruding vulva. Maternal effect: progeny of dpy-27 homozygotes will be severely Dpy, marginally viable, and their progeny will be more severe Dpy hermaphrodites and WT males. Males are vigorous and mate well. See also WBPaper00002061. Mutagen: Outcrossed: x Made by: Edgley M Received: 01/20/92 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1564 Species: Caenorhabditis elegans Genotype: daf-2(m41) III. Description: Temperature-sensitive dauer-consitutive, long-lived, intrinsically thermotolerant. Adults slightly shorter than adults of other daf-2 alleles. Up to 6% of L1s arrest at 25.5C. Makes nearly 100% dauers at 25C, 0% dauers at 15C. Good recovery of dauers at 15C. Mutagen: Outcrossed: x3 Made by: David Gems Received: 09/05/97 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1565 Species: Caenorhabditis elegans Genotype: daf-2(m596) III. Description: Class 2 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt). Mutagen: Outcrossed: x3 Made by: Received: 12/30/99 University College, London, England ----------------------------------------------------------------------------- Strain: DR1566 Species: Caenorhabditis elegans Genotype: daf-2(m579) III. Description: Adults Age and Itt and temperature sensitive Unc (class 2 allele). m579 results in an amino acid substitution(R437C) in the extracellular portion of the DAF-2 receptor (specifically, the Cys-rich domain). Mutagen: EMS Outcrossed: x3 Made by: Riddle Lab Received: 10/09/08 University College, London, England ----------------------------------------------------------------------------- Strain: DR1567 Species: Caenorhabditis elegans Genotype: daf-2(m577) III. Description: Class 1 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt). Mutagen: Outcrossed: x3 Made by: Received: 12/30/99 University College, London, England ----------------------------------------------------------------------------- Strain: DR1568 Species: Caenorhabditis elegans Genotype: daf-2(e1371) III. Description: Class 1 allele of daf-2. Temperature sensitive Daf-c. Maintain at 15C. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt). Mutagen: Outcrossed: x3 Made by: Received: 12/30/99 University College, London, England ----------------------------------------------------------------------------- Strain: DR1572 Species: Caenorhabditis elegans Genotype: daf-2(e1368) III. Description: Maintain at 15C.  Class 1 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt). Mutagen: N/A Outcrossed: x3 Made by: Received: 12/30/99 University College, London, England ----------------------------------------------------------------------------- Strain: DR1574 Species: Caenorhabditis elegans Genotype: daf-2(e1391) III. Description: Class 2 allele of daf-2. Temperature sensitive Daf-c. Adults are long-lived (Age) and exhibit extrinsic thermotolerance (Itt). Produces some dauers at 15C. CGC received new stock 10/25/00 from David Gems. Received new stock from Patrice Albert in the Riddle lab 6/04. Mutagen: Outcrossed: x3 Made by: David Gems Received: 12/30/99 University College, London, England ----------------------------------------------------------------------------- Strain: DR168 Species: Caenorhabditis elegans Genotype: unc-13(e51) daf-8(e1393) I. Description: Temperature sensitive dauer constitutive. Unc. Semi-paralyzed. Closely linked. Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR169 Species: Caenorhabditis elegans Genotype: che-3(e1378) I; unc-5(e53) IV. Description: Dauer defective. Unc-coiler. Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1690 Species: Caenorhabditis briggsae Genotype: C. briggsae. Description: Previously called C. briggsae Zuckerman. This stock was maintained in liquid culture for some number of years and carrues a 33-kilobase deletion that disrupts one of the srg paralogs, CBG24690, and six other genes. It is Unc, dauer-defective and ts lethal. Reference: McGrath PT, et al. Nature. 2011 Aug 17;477(7364):321-5. Mutagen: Outcrossed: x Made by: Received: 02/01/81 ----------------------------------------------------------------------------- Strain: DR170 Species: Caenorhabditis elegans Genotype: unc-5(e53) IV; che-2(e1033) X. Description: Dauer defective. Unc-coiler. Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1720 Species: Caenorhabditis elegans Genotype: unc-4(e120) daf-19(m86) II. Description: Unc. Dauer constitutive. Mutagen: Outcrossed: x Made by: Patrice Albert Received: 05/08/97 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR176 Species: Caenorhabditis elegans Genotype: unc-54(e190) I; eDp23 V. Description: Suppressed-movement slow. Unlinked double. Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR177 Species: Caenorhabditis elegans Genotype: unc-54(e1258) I; eDp22 V. Description: Suppressed-movement slow. Mutagen: Outcrossed: x Made by: Riddle D Received: 06/01/76 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1785 Species: Caenorhabditis elegans Genotype: mIn1 [dpy-10(e128)]/unc-4(e120) II. Description: WT phenotype. Segregates WT, homozygous Dpy-10 mIn1 and homozygous Unc-4 hermaphrodites. Recombination in this interval is suppressed, and recombinant animals have not been detected in this stock. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to Don Riddle. mIn1 pka mC6. Mutagen: Gamma Rays Outcrossed: x3 Made by: Mark Edgley Received: 09/05/97 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1786 Species: Caenorhabditis elegans Genotype: dpy-13(e184) unc-24(e138) IV; mDp4[unc-17(e245)] (IV;?). Description: WT phenotype. Segregates WT and DpyUncs. mDp4 carries dpy-13(+) and unc-24(+). Duplication may recombine with normal homologues. Presence of unc-17(e245) on mDp4 confirmed: got Unc-17 segregants after heat shock of this stock to generate males. Pick WT and check for segregation of progeny to maintain. Mutagen: Gamma Rays Outcrossed: x Made by: Mark Edgley Received: 09/17/98 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1790 Species: Caenorhabditis elegans Genotype: rol-1(e91)/mT1 II; unc-71(e541)/mT1 [dpy-10(e128)] III. Description: Heterozygotes are WT and segregate WT, Roller Uncs, and a few Dpy mT1 homozygotes. mT1 is an apparent II;III translocation: small broods, lots of dead eggs, and exhibits pseudolinkage between rol-1 II and unc-71 III. The Roller phenotype of rol-1 expresses late and cannot be scored before adulthood. Pick non-Unc hermaphrodites and check for correct segregation of progeny. Mutagen: Gamma Rays: mT1 Outcrossed: x1 Made by: Mark Edgley Received: 03/22/02 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1799 Species: Caenorhabditis elegans Genotype: daf-7(n696)/unc-45(st604) III. Description: WT phenotype. Segregates WT, constitutive dauers and ts mild Uncs. Uncs are maternal-effect lethal: F1 Uncs from a heterozygote produce only arrested 2-fold stage progeny that hatch. Grow at 22.5C (n696 produces nearly 100% dauers at this temperature). Mutagen: Outcrossed: x1 Made by: Mark Edgley Received: 05/08/97 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1809 Species: Caenorhabditis elegans Genotype: unc-4(e120)/mIn1 [dpy-10(e128) let-?(m727)] II. Description: Heterozygotes are WT and segregate WT, unc-4, and lethal mIn1 homozygotes. Pick WT and check for correct segregation of progeny. Mutagen: UV Outcrossed: x2 Made by: Edgley/Turner Received: 03/22/02 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR181 Species: Caenorhabditis elegans Genotype: unc-60(m35) dpy-11(e224) V. Description: Dpy. Unc-slow moving. Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR183 Species: Caenorhabditis elegans Genotype: dpy-11(e224) daf-11(m47) V. Description: Temperature sensitive dauer constitutive. Dpy. Mutagen: Outcrossed: x Made by: Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1832 Species: Caenorhabditis elegans Genotype: mT1/unc-4(e120) II; mT1 [dpy-10(e128)]/dpy-17(e164) III. Description: WT phenotype. Segregates WT, sterile Dpy mT1 homozygotes, Unc-4;Dpy-17 and large numbers of arrested aneuploid embryos. Pick WT and check for correct segregation of progeny to maintain. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to Don Riddle. Mutagen: Gamma Rays Outcrossed: x2 Made by: Mark Edgley Received: 09/05/97 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR184 Species: Caenorhabditis elegans Genotype: daf-8(e1393) I; daf-10(e1387) IV. Description: Temperature sensitive dauer constitutive. Dauer recovery poor at 15C. Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR185 Species: Caenorhabditis elegans Genotype: dpy-13(e184) unc-22(m52) IV. Description: Dominant Twitcher Unc. Semi-dominant Dpy. Mutagen: Outcrossed: x Made by: MacMorris M Received: 11/01/77 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR188 Species: Caenorhabditis elegans Genotype: daf-11(m47) unc-76(e911) V. Description: Temperature sensitive dauer constitutive. Dauer recovery poor at 15C. Unc. Mutagen: Outcrossed: x Made by: MacMorris M Received: 01/01/78 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR189 Species: Caenorhabditis elegans Genotype: dpy-5(e61) daf-8(e1393) unc-29(e1072) I. Description: Temperature sensitive dauer constitutive. Dpy. Unc. Mutagen: Outcrossed: x Made by: MacMorris M Received: 01/01/78 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR190 Species: Caenorhabditis elegans Genotype: dpy-13(e184) unc-24(e138) IV. Description: Dpy. Unc. Mutagen: Outcrossed: x Made by: MacMorris M Received: 01/01/78 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR194 Species: Caenorhabditis elegans Genotype: dpy-11(e224) daf-11(m47) unc-39(e257) V. Description: Temperature sensitive dauer constitutive. Leaky. Dpy. Unc. Mutagen: Outcrossed: x Made by: MacMorris M Received: 02/01/78 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR1942 Species: Caenorhabditis elegans Genotype: daf-2(e979) III. Description: This strain forms 20% dauers at 15C. At 25C there occurs about 25% embryonic arrest and about 75% L1 arrest. The e979 mutation results in an amino acid substitution, C146Y, in the ligand-binding domain of the DAF-2 receptor. [CGC received new stock of DR1942 September 2002. Previous stock was probably m41 and not e979.] [June 2004: Patrice Albert has confirmed the mutation in this stock: Repeat of sequencing for CGC collection strain DR1942 [daf-2(e979)] is complete. The strain does carry a C146Y mutation (coding strand TGC to TAC) [Mutation position is at 143, not 146, based on the amino acid sequence shown in Wormbase for daf-2. It's the C in partial sequence EKRCGPI of Exon 5.].] Mutagen: Outcrossed: x3 Made by: Received: 09/20/02 University College, London, England ----------------------------------------------------------------------------- Strain: DR195 Species: Caenorhabditis elegans Genotype: dpy-5(e61) daf-16(m26) I. Description: Dauer defective. Leaky. Dpy. Mutagen: Outcrossed: x Made by: MacMorris M Received: 02/01/78 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR196 Species: Caenorhabditis elegans Genotype: daf-1(m40) dpy-13(e184) IV. Description: Temperature sensitive dauer constitutive . Dpy. Maternal effect. Mutagen: Outcrossed: x Made by: Riddle D Received: 12/01/77 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR2 Species: Caenorhabditis elegans Genotype: unc-26(m2) IV. Description: Kinky. Coiler. Mutagen: DES Outcrossed: x Made by: Riddle D Received: 01/01/76 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR20 Species: Caenorhabditis elegans Genotype: daf-12(m20) X. Description: Defective dauer formation. Hermaphrodites accumulate oocytes. Males mate poorly. Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR200 Species: Caenorhabditis elegans Genotype: daf-2(e1370) III; daf-13(m66) X. Description: Temperature sensitive dauer constitutive. Dauers SDS-sensitive. Mutagen: Outcrossed: x Made by: Kirsh M Received: 06/01/78 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR201 Species: Caenorhabditis elegans Genotype: dpy-13(e184) daf-14(m77) IV. Description: Temperature sensitive dauer constitutive. Dpy. Leaky double at 25C. Mutagen: Outcrossed: x Made by: Albert P Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR202 Species: Caenorhabditis elegans Genotype: unc-24(e138) daf-14(m77) IV. Description: Temperature sensitive dauer constitutive. Unc. Mutagen: Outcrossed: x Made by: Albert P Received: 05/01/78 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR205 Species: Caenorhabditis elegans Genotype: lon-2(e678) daf-12(m20) X. Description: Dauer defective. Long. Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR2054 Species: Caenorhabditis elegans Genotype: mIn1 [unc-4(e120) dpy-10(e128)]/let-552(e2542) rol-1(e91) II. Description: Heterozygotes are WT and segregate WT, DpyUnc [mIn1(mc6) homozygotes], and two-fold arrest Let Rol progeny. Well balanced. Male stock. Cross WT males to WT hermaphrodites and check for correct segregation of progeny. Mutagen: Outcrossed: x1 Made by: Mark Edgley Received: 03/22/02 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR2055 Species: Caenorhabditis elegans Genotype: mIn1(+) II. Description: Dark-bodied, unmarked variant of mIn19mC6) with small broods. Presumably balances let-552 to rol-1 (hermaphrodite stock obtained from outcrossing these males to dpy-10 unc-4 was shown to balance this interval). Isolated from DR1982 as a stock that no longer segregated Dpy-10 animals. Not clear whether this strain is the result of chromosome rearrangement in DR1982, or if DR1982 stock was a mixture of mIn1(+)/mIn[dpy-10] and mIn1(+) homozygotes. Mutagen: UV Outcrossed: x2 Made by: Mark Edgley Received: 03/22/02 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR206 Species: Caenorhabditis elegans Genotype: unc-87(e1459) I; eDp23 V. Description: Suppressed Unc. Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR2075 Species: Caenorhabditis elegans Genotype: mIn1 [unc-4(e120)]/let-552(e2542) rol-1(e91) II. Description: Heterozygotes are WT and segregate WT, Lethal Rollers (arrested 2-fold hatchlings), and Unc-4 mIn1(mC6) homozygotes. Pick WT and check for correct segregation of progeny. Mutagen: Outcrossed: x Made by: Mark Edgley Received: 03/22/02 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR2078 Species: Caenorhabditis elegans Genotype: mIn1 [dpy-10(e128) mIs14]/bli-2(e768) unc-4(e120) II. Description: WT gross phenotype, with GFP semi-dominantly expressed in 4-60 cell embyros, pharyngeal muscle and gut. Segregates WT, brighter Dpy GFP mIn1 homozygotes and non-GFP bli-2 unc-4 homozygotes. Pharyngeal and gut GFP is easily seen in a UV dissecting microscope; early embryonic signal requires higher magnification. mIs14 occasionally crosses off mIn1[dpy-10], apparently by double recombination. Pick WT, check for GFP and check for correct segregation of progeny to maintain. mIs14 is ccEx9747 integrated into mIn1[dpy-10]. This is a three-construct element containing myo-2 and pes-10 promoters and a gut enhancer fused individually to GFP coding sequence. Mutagen: Outcrossed: x4 Made by: Mark Edgley Received: 03/22/99 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR210 Species: Caenorhabditis elegans Genotype: dpy-5(e61) daf-16(m26) unc-75(e950) I. Description: Dauer defective. Dpy. Unc. Leaky. Mutagen: Outcrossed: x Made by: MacMorris M Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR2102 Species: Caenorhabditis elegans Genotype: mIn1 [rol-1(e91)]/let-552(e2542) unc-4(e120) II. Description: Heterozygotes are WT and segregate WT, Lethal Uncs and Roller mIn1 homozygotes. let-552 unc-4 chromosome is well balanced. Rol does not express until late L4/early adult. Pick WT and check for correct segregation of progeny. Mutagen: Outcrossed: x Made by: Mark Edgley Received: 03/22/02 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR211 Species: Caenorhabditis elegans Genotype: daf-16(m26) unc-75(e950) I. Description: Dauer defective. Unc. Mutagen: Outcrossed: x Made by: MacMorris M Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR215 Species: Caenorhabditis elegans Genotype: unc-1(e94) daf-13(m66) X. Description: Dauers SDS-sensitive. Unc. Mutagen: Outcrossed: x Made by: Albert P Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR2150 Species: Caenorhabditis elegans Genotype: mIn1 [rol-1(e91) dpy-10(e128)]/let-552(e2542) unc-4(e120) II. Description: Heterozygotes are WT and segregate WT, DpyRol mIn1 homozygotes and 2-fold arrest Unc larvae. Male stock; mate WT males and WT hermaphrodites to maintain. Mutagen: Outcrossed: x2 Made by: Mark Edgley Received: 03/22/02 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR2153 Species: Caenorhabditis elegans Genotype: rrf-3(b26) II; liv-4(m872) V. Description: Weak Unc (kinker) as adult. Increased life span. Maintain at 15C. Mutagen: EMS Outcrossed: x3 Made by: Manuel Munoz Received: 09/29/03 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR2168 Species: Caenorhabditis elegans Genotype: liv-2(m882) rrf-3(b26) II. Description: Approximately 30% dauer at 27C. Probably thermotolerant because it comes from a thermotolerant selection, but it was not retested. Increaesed longevity. Maintain at 15C. Mutagen: EMS Outcrossed: x3 Made by: Manuel Munoz Received: 09/29/03 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR2203 Species: Caenorhabditis elegans Genotype: daf-16(m26) I; rrf-3(b26) liv-2(m882) II. Description: daf-d. Maintain at 15C. Mutagen: Outcrossed: x Made by: Manuel Munoz Received: 10/03/03 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR2208 Species: Caenorhabditis elegans Genotype: daf-16(m26) I; rrf-3(b26) II; liv-4(m872) V. Description: daf-d on starvation. Sterile at 25C. Maintain at 15C. Mild Unc. Mutagen: Outcrossed: x Made by: Manuel Munoz Received: 09/29/03 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR222 Species: Caenorhabditis elegans Genotype: him-1(e879) I; dpy-13(e184) IV. Description: Dpy. Segregates males. Mutagen: Outcrossed: x Made by: MacMorris M Received: 07/01/78 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR223 Species: Caenorhabditis elegans Genotype: him-1(e879) I; sqt-1(e1350) II. Description: Dpy. Segregates males. Semi-dominant Roller. Mutagen: Outcrossed: x Made by: MacMorris M Received: 07/01/78 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR224 Species: Caenorhabditis elegans Genotype: daf-1(m40) unc-33(e204) IV. Description: Temperature sensitive dauer constitutive. Unc. Mutagen: Outcrossed: x Made by: MacMorris M Received: 07/01/78 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR226 Species: Caenorhabditis elegans Genotype: him-1(e879) unc-54(m36) I. Description: Unc. Semi-parlayzed. Segregates males. Heterozygotes are slow moving. Mutagen: Outcrossed: x Made by: MacMorris M Received: 08/01/78 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR2278 Species: Caenorhabditis elegans Genotype: aap-1(m889) I. Description: Dauer formation at 27C. Long-lived. Maternal rescue. Mutagen: Outcrossed: x4 Made by: Monica Gallas Received: 01/22/03 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR2281 Species: Caenorhabditis elegans Genotype: daf-9(m540) X. Description: Forms dauer larvae non-conditionally but only arrests at dauer stage for 1-2 days, then grows to adult. daf-c but not ts - can grow at 15C or 20C. Mutagen: Outcrossed: x Made by: Kailiang Jia Received: 10/03/03 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR234 Species: Caenorhabditis elegans Genotype: dpy-5(e61) daf-16(m27) I. Description: Dauer defective. Dpy. Mutagen: Outcrossed: x Made by: Albert P Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR238 Species: Caenorhabditis elegans Genotype: dpy-11(e224) mec-9(e1494) V. Description: Dpy. Mechanosensory abnormal. Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR2386 Species: Caenorhabditis elegans Genotype: daf-25(m362) I. Description: Maintain at 15C. Daf-C. Reference: Jensen VL, et al. PLoS Genet. 2010 Nov 24;6(11):e1001199. Mutagen: EMS Outcrossed: x2 Made by: J Golden & P Albert Received: 10/16/13 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: DR240 Species: Caenorhabditis elegans Genotype: dpy-11(e224) unc-42(e270) daf-11(m84) V. Description: Temperature sensitive dauer constitutive. Dpy. Unc. Mutagen: Outcrossed: x Made by: Riddle D Received: 07/01/79 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR244 Species: Caenorhabditis elegans Genotype: daf-19(m86) sqt-1(e1350) II. Description: Temperature sensitive dauer constitutive. Many dauers at 15C. Dpy. Heterozygotes Roll. Mutagen: Outcrossed: x Made by: Riddle/Albert Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR245 Species: Caenorhabditis elegans Genotype: daf-14(m77) unc-22(m52) IV. Description: Temperature sensitive dauer constitutive. Dominant Twitcher Mutagen: Outcrossed: x Made by: Albert P Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR25 Species: Caenorhabditis elegans Genotype: daf-12(m25) X. Description: Dauer defective. Class 2 suppressor of dauer constitutives. Chemotaxis normal. See also WBPaper00002149. [Previously called daf-20.] Mutagen: Outcrossed: x Made by: Riddle D Received: 01/01/76 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR26 Species: Caenorhabditis elegans Genotype: daf-16(m26) I. Description: Dauer defective-leaky. Somewhat small. Suppresses daf-2. Mutagen: Outcrossed: x Made by: Riddle D Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR27 Species: Caenorhabditis elegans Genotype: daf-16(m27) I. Description: Dauer defective. Chemotaxis normal. Class 2 suppressor of dauer constitutive. Mutagen: Outcrossed: x Made by: Riddle D Received: 06/01/76 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR281 Species: Caenorhabditis elegans Genotype: unc-31(e169) dpy-4(e1166) IV. Description: Dpy. Unc-slow moving. Mutagen: Outcrossed: x Made by: Brown S Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR282 Species: Caenorhabditis elegans Genotype: dpy-13(e184) unc-31(e169) IV. Description: Dpy. Unc-slow moving. Mutagen: Outcrossed: x Made by: Brown S Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR284 Species: Caenorhabditis elegans Genotype: unc-32(e189) III; him-8(e1489) IV. Description: Unc-coiler. Segregates males. M-MATING-NO SUCCESS. Mutagen: Outcrossed: x Made by: MacMorris M Received: 09/01/79 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR285 Species: Caenorhabditis elegans Genotype: sqt-1(e1350) II; him-8(e1489) IV. Description: Dpy. Heterozygotes Roll. Segregates males. M-MATING-NO SUCCESS. Mutagen: Outcrossed: x Made by: MacMorris M Received: 09/01/79 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR286 Species: Caenorhabditis elegans Genotype: him-8(e1489) IV; unc-58(e665) X. Description: Semi-dominant Unc-shaker. Segregates males. Mutagen: Outcrossed: x Made by: MacMorris M Received: 09/01/79 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR288 Species: Caenorhabditis elegans Genotype: dpy-13(e184) him-8(e1489) IV. Description: Dpy. Segregates males. Semi-dominant. Mutagen: Outcrossed: x Made by: MacMorris M Received: 09/01/79 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR290 Species: Caenorhabditis elegans Genotype: unc-54(m36) I; him-8(e1489) IV. Description: Severe Unc. Segregates males. M-MATING-NO SUCCESS. Semi-dominant. Heterozygotes are slow moving. Mutagen: Outcrossed: x Made by: MacMorris M Received: 10/01/79 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR291 Species: Caenorhabditis elegans Genotype: unc-54(e675) I; eDp23 V. Description: Not suppressed. Unc-slow moving. Mutagen: Outcrossed: x Made by: Brown S Received: 12/01/79 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR292 Species: Caenorhabditis elegans Genotype: che-3(e1379) I; him-8(e1489) IV. Description: Dauer defective. Segregates males. Chemotaxis defective (Na+). Mutagen: Outcrossed: x Made by: Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR293 Species: Caenorhabditis elegans Genotype: dpy-5(e61) unc-101(m1) I. Description: Dpy. Unc-paralyzed coiler. Mutagen: Outcrossed: x Made by: Golden J Received: 01/01/80 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR297 Species: Caenorhabditis elegans Genotype: unc-15(e1402) I; eDp23 V. Description: Mutagen: Outcrossed: x Made by: Brown S Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR31 Species: Caenorhabditis elegans Genotype: unc-54(m31) I. Description: Severe Unc. Very slow growth. Strong semi-dominant. Heterozygous males don't mate. Mutagen: EMS Outcrossed: x Made by: Riddle D Received: 12/01/76 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR32 Species: Caenorhabditis elegans Genotype: unc-54(m32) I. Description: Severe Unc. Growth slow. Recessive. Mutagen: EMS Outcrossed: x Made by: Riddle D Received: 12/01/76 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR33 Species: Caenorhabditis elegans Genotype: unc-54(m33) I. Description: Movement very slow. Recessive. Mutagen: EMS Outcrossed: x Made by: Riddle D Received: 12/01/76 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR34 Species: Caenorhabditis elegans Genotype: unc-54(m34) I. Description: Moves relatively well. Recessive. Mutagen: EMS Outcrossed: x Made by: Riddle D Received: 12/01/76 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR35 Species: Caenorhabditis elegans Genotype: unc-60(m35) V. Description: Moves slowly. Recessive. Lays eggs. Mutagen: EMS Outcrossed: x Made by: Riddle D Received: 12/01/76 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR36 Species: Caenorhabditis elegans Genotype: unc-54(m36) I. Description: Severe homozygous Unc. Heterozygotes are slow moving. Weakly semi-dominant. Body muscle abnormal. Mutagen: EMS Outcrossed: x Made by: Riddle D Received: 12/01/76 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR37 Species: Caenorhabditis elegans Genotype: unc-54(m37) I. Description: Healthy. Recessive. Dauer recovery slow. Eggs laid rarely. Mutagen: EMS Outcrossed: x Made by: Riddle D Received: 12/01/76 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR373 Species: Caenorhabditis elegans Genotype: unc-15(e73) I; sus-1(m156) III; eDp23 V. Description: Paralyzed Unc. sus-1 suppresses the ability of eDp23 to suppress unc-15. eDp23 = sup-3(e1407). Mutagen: Outcrossed: x Made by: Brown S Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR38 Species: Caenorhabditis elegans Genotype: dpy-7(m38) X. Description: Temperature sensitive Dpy. Penetrance is incomplete. Left hand roller. Roller low penetrance. Maternal sterile at 25C. Mutagen: EMS Outcrossed: x Made by: Riddle D Received: 12/01/76 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR39 Species: Caenorhabditis elegans Genotype: dpy-3(m39) X. Description: Temperature sensitive Dpy. Penetrance is incomplete. Left hand roller. Roller low penetrance. Grows well at 25C. Mutagen: EMS Outcrossed: x Made by: Riddle D Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR40 Species: Caenorhabditis elegans Genotype: daf-1(m40) IV. Description: Temperature sensitive dauer constitutive. 100% dauers at 25C. Dauer recovery poor at 15C. Maternal effect. Egg retainer. Mutagen: EMS Outcrossed: x Made by: Riddle D Received: 11/01/78 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR401 Species: Caenorhabditis elegans Genotype: unc-15(e73) I; eDf1 eDp21/dpy-11(e224) V. Description: Heterozygotes are slow moving. Hets segregate DpyUncs. Hets segregate larval lethals (eDf1 eDp21 homozygotes). Mutagen: Outcrossed: x Made by: Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR403 Species: Caenorhabditis elegans Genotype: eDf1 eDp21/sma-1(e30) V. Description: Heterozygotes are WT and segregate WT, Sma and lethals which die in larval development. Mutagen: Outcrossed: x Made by: Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR404 Species: Caenorhabditis elegans Genotype: unc-54(e1258) I; eDf1 eDp21/+ V. Description: Mutagen: Outcrossed: x Made by: Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR405 Species: Caenorhabditis elegans Genotype: unc-54(e190) I; eDf1 eDp21/+ V. Description: Mutagen: Outcrossed: x Made by: Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR410 Species: Caenorhabditis elegans Genotype: daf-2(m65)/unc-32(e189) III. Description: Heterozygotes are WT and segregate WT, dauer constitutive, and Uncs. Lethal dauers. Mutagen: Outcrossed: x Made by: Received: 07/01/78 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR411 Species: Caenorhabditis elegans Genotype: dpy-13(e184)/daf-15(m81) IV. Description: Heterozygotes are semi-Dpy and segregate semi-Dpy, Dpy and dauers. Dauers are lethal and SDS-sensitive. NOTE: WT recombinants that have lost dpy-13 can quickly overtake a population. Mutagen: Outcrossed: x Made by: Received: 03/01/81 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR412 Species: Caenorhabditis elegans Genotype: daf-15(m81)/unc-24(e138) IV. Description: Heterozygotes are WT and segregate WT, Unc, and lethal dauers. Dauers are SDS-sensitive. Mutagen: EMS Outcrossed: x Made by: Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR427 Species: Caenorhabditis elegans Genotype: daf-12(m116) X. Description: daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele. Mutagen: EMS Outcrossed: x Made by: Don Riddle Received: 10/23/00 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: DR429 Species: Caenorhabditis elegans Genotype: dpy-5(e61) unc-15(e73) I. Description: Dpy. Unc. Semi-paralyzed. Mutagen: Outcrossed: x Made by: Brown S Received: 06/01/81 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR431 Species: Caenorhabditis elegans Genotype: daf-19(m86)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, dauers and DpyUnc. Mutagen: Outcrossed: x Made by: MacMorris M Received: 06/01/81 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR432 Species: Caenorhabditis elegans Genotype: ama-1(m118) IV. Description: Mutagen: Outcrossed: x Made by: Sanford/Riddle Received: 02/01/83 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR435 Species: Caenorhabditis elegans Genotype: dpy-5(e61) unc-13(e51) I. Description: Dpy. Unc. Mutagen: Outcrossed: x Made by: MacMorris M Received: 08/01/81 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR436 Species: Caenorhabditis elegans Genotype: dpy-5(e61) unc-14(e57) I. Description: Dpy. Unc. Mutagen: Outcrossed: x Made by: MacMorris M Received: 08/01/81 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR437 Species: Caenorhabditis elegans Genotype: dpy-5(e61) unc-29(e1072) I. Description: Dpy. Unc. Mutagen: Outcrossed: x Made by: MacMorris M Received: 08/01/81 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR438 Species: Caenorhabditis elegans Genotype: dpy-5(e61) unc-87(e1216) I. Description: DpyUnc strain. Mutagen: Outcrossed: x Made by: MacMorris M Received: 08/01/81 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR439 Species: Caenorhabditis elegans Genotype: unc-8(e49) dpy-20(e1282) IV. Description: DpyUnc. Mutagen: Outcrossed: x Made by: Wilke K Received: 09/01/81 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR441 Species: Caenorhabditis elegans Genotype: lin-14(n179) X. Description: Vulva abnormal. Temperature sensitive. Received new stock from Frank Slack's lab on March 23, 2007. Mutagen: Spontaneous Outcrossed: x Made by: Fodor A Received: 09/01/81 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR442 Species: Caenorhabditis elegans Genotype: unc-8(e49) daf-14(m77) IV. Description: Temperature sensitive dauer constitutive. Unc. Mutagen: Outcrossed: x Made by: Wilke K Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR443 Species: Caenorhabditis elegans Genotype: dpy-14(e188) daf-8(e1393) I. Description: Temperature sensitive dauer constitutive. Dpy. Mutagen: Outcrossed: x Made by: Wilke K Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR466 Species: Caenorhabditis elegans Genotype: him-5(e1490) V. Description: Approx. 33% males. Mutagen: EMS Outcrossed: x Made by: MacMorris M Received: 01/01/82 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR47 Species: Caenorhabditis elegans Genotype: daf-11(m47) V. Description: Temperature sensitive. Leaky at 25C. Dauers recover poorly at 15C. Dauers escape plates. Recessive. Chemotaxis defective (Na+). Received new stock from Riddle lab in November 2006. Mutagen: EMS Outcrossed: x Made by: Riddle D Received: 12/01/76 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR476 Species: Caenorhabditis elegans Genotype: daf-22(m130) II. Description: Dauer defective. Mutagen: EMS Outcrossed: x Made by: Golden J Received: 02/01/83 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR478 Species: Caenorhabditis elegans Genotype: unc-13(e450) I; sup-7(st5) X. Description: sup-7 suppresses unc-13. Movement not WT. Fertility is low. Grows between 22.5C and 24C. Mutagen: EMS Outcrossed: x Made by: Golden J Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: DR49 Species: Caenorhabditis elegans Genotype: tln-1(e259) I. Description: Unc. Weakly semi-dominant. Scored with difficulty. Mutagen: EMS Outcrossed: x Made by: Beguet B Received: 09/01/77 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR497 Species: Caenorhabditis elegans Genotype: unc-13(e450) I; daf-7(m62) III; sup-7(st5) X. Description: Both unc-13 and daf-7 are suppressed by sup-7. sup-7 should be grown between 22.5C and 24C. Mutagen: Outcrossed: x Made by: Golden J Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: DR508 Species: Caenorhabditis elegans Genotype: unc-13(e450) I; daf-4(m72) III; sup-7(st5) X. Description: sup-7 suppress dauer formation. sup-7 should be grown between 22.5C and 24C. Mutagen: Outcrossed: x Made by: Golden J Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: DR512 Species: Caenorhabditis elegans Genotype: che-11(m162) V. Description: Mutagen: EMS Outcrossed: x Made by: Received: 11/15/99 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DR518 Species: Caenorhabditis elegans Genotype: rol-6(su1006) unc-4(e120) II. Description: RollerUnc. Mutagen: Outcrossed: x Made by: Edgley M Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR52 Species: Caenorhabditis elegans Genotype: unc-22(m52) IV. Description: Unc-Twitcher. Dominant. Genotype questionable. Mutagen: EMS Outcrossed: x Made by: MacMorris M Received: 10/01/77 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR520 Species: Caenorhabditis elegans Genotype: sDf9/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, and lethals. Lethal early larval. Heterozygotes twitch in 1% nicotine. Well balanced. Mutagen: Formaldehyde Outcrossed: x Made by: MacMorris M Received: 07/01/82 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR54 Species: Caenorhabditis elegans Genotype: unc-22(m54) IV. Description: Strong Twitcher. Recessive. Mutagen: EMS Outcrossed: x Made by: Received: 05/01/78 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR561 Species: Caenorhabditis elegans Genotype: sDf8/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul and lethals. Lethal early larval. Heterozygotes twitch in 1% nicotine. Mutagen: Formaldehyde Outcrossed: x Made by: Wilke K Received: 08/01/82 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR6 Species: Caenorhabditis elegans Genotype: unc-1(m6) X. Description: Kinky Unc. Mutagen: Nitrosoguanidine Outcrossed: x Made by: Riddle D Received: 01/01/76 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR605 Species: Caenorhabditis elegans Genotype: unc-15(e73) I; sup-19(m210) V. Description: Unc phenotype weakly suppressed. Very slow. Mutagen: EMS Outcrossed: x Made by: Brown S Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: DR608 Species: Caenorhabditis elegans Genotype: daf-2(m212) III. Description: Temperature sensitive Daf-c. Maintain at 15C. Adults Age and Itt, but not Unc (severe class 1 allele). m212 results in an amino acid substitution(C883Y) in the extracellular portion of the DAF-2 receptor (specificaly, the FnIII2ID domain). Mutagen: EMS Outcrossed: x Made by: Riddle Lab Received: 10/09/08 University College, London, England ----------------------------------------------------------------------------- Strain: DR609 Species: Caenorhabditis elegans Genotype: daf-1(m213). Description: Temperature sensitive dauer constitutive. Mutagen: Outcrossed: x Made by: Schranck Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: DR62 Species: Caenorhabditis elegans Genotype: daf-7(m62) III. Description: Temperature sensitive dauer constitutive. 100% dauers at 25C. Some dauers (3.5%) at 15C. Mutagen: EMS Outcrossed: x Made by: MacMorris M Received: 09/01/78 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR629 Species: Caenorhabditis elegans Genotype: unc-15(e73) I; sus-1(m156) daf-2(e1370) III; eDp23 V. Description: Temperature sensitive dauers. Paralyzed Unc. sus-1 suppresses the ability of eDp23 to suppress unc-15. eDp23 = sup-3(e1407). Mutagen: Outcrossed: x Made by: Brown S Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR63 Species: Caenorhabditis elegans Genotype: daf-4(m63) III. Description: Temperature sensititve dauer constitutive. Small. 100% dauers at 25C. 15% dauers at 15C. Mutagen: EMS Outcrossed: x Made by: MacMorris M Received: 03/01/78 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR631 Species: Caenorhabditis elegans Genotype: unc-54(e190) I; sus-1(m156) III. Description: Severly paralyzed Unc. More severe than unc-15 alone. Mutagen: Outcrossed: x Made by: Brown S Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR632 Species: Caenorhabditis elegans Genotype: unc-15(e73) I; sus-1(m156) III. Description: Severly ill and paralyzed. Lays few eggs. More severe than unc-15 alone. Mutagen: Outcrossed: x Made by: Brown S Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR640 Species: Caenorhabditis elegans Genotype: ama-1(m118) unc-5(e53) IV. Description: Hypercontracted and amanitin resistant. Mutagen: Outcrossed: x Made by: Haase C Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: DR641 Species: Caenorhabditis elegans Genotype: ama-1(m118m221) unc-8(e15)/nT1 IV; +/nT1 V. Description: Heterozygotes are semi-Unc and segregate semi-Unc and Vul and dead eggs. (Does not segregate Unc homozygotes because linked to the unc is a lethal in the amanitin gene.) Mutagen: Outcrossed: x Made by: Haase C Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: DR645 Species: Caenorhabditis elegans Genotype: ama-1(m118) unc-8(e15) IV. Description: Mutagen: Outcrossed: x Made by: Albert P Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: DR66 Species: Caenorhabditis elegans Genotype: daf-13(m66) X. Description: SDS sensitive dauers. Non-crowder. Mutagen: EMS Outcrossed: x Made by: Albert P Received: 04/01/78 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR68 Species: Caenorhabditis elegans Genotype: unc-31(m68) IV. Description: Slow movement. Mutagen: EMS Outcrossed: x Made by: Riddle D Received: 05/01/78 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR680 Species: Caenorhabditis elegans Genotype: dpy-13(e184) ama-1(m118) IV. Description: Dpy phenotype ranges from very tiny to normal dpy-13. Resistant to alpha-amanitin. Mutagen: Outcrossed: x Made by: Rogalski T Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: DR682 Species: Caenorhabditis elegans Genotype: dpy-13(e184) ama-1(m118m235) IV/nT1 [qIs51] (IV;V). Description: Temperature sensitive. Sensitive to alpha-amanitin. Heterozygotes are semi-Dpy and segregate semi-Dpy, Vul, dead embryos, and Dpy. Dpy progeny arrest in mid-larval stages at >20C. Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416.Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416. Mutagen: EMS Outcrossed: x0 Made by: Teresa Rogalski Received: 11/01/11 Emory University, Atlanta, GA ----------------------------------------------------------------------------- Strain: DR683 Species: Caenorhabditis elegans Genotype: dpy-13(e184) ama-1(m118m236)/nT1 IV; +/nT1 V. Description: Temperature sensitive . Sensitive to alpha-amanitin. Heterozygotes are semi-Dpy and segregate semi-Dpy, Vul, dead eggs and Dpys. Dpys are sterile adults at 15C and 20C. Dpys are early larval lethals at 25C. Mutagen: EMS Outcrossed: x Made by: Rogalski T Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: DR684 Species: Caenorhabditis elegans Genotype: mDf9/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul and dead eggs. Homozygous Df is embryonic lethal. Strain is sensitive to alpha-amanitin. New stock received from Patrice Albert 12/95. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to Don Riddle. Mutagen: EMS Outcrossed: x Made by: Rogalski T Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR685 Species: Caenorhabditis elegans Genotype: dpy-13(e184) ama-1(m118m238)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, Dpy and dead eggs. Dpys produce progeny at 15C and 20C, but are sterile at 25C. Strain is sensitive to alpha-amanitin. Mutagen: EMS Outcrossed: x Made by: Rogalski T Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR690 Species: Caenorhabditis elegans Genotype: unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-272(m243) IV. Description: Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc, and DpyLet. Mutagen: EMS Outcrossed: x Made by: Rogalski T Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR691 Species: Caenorhabditis elegans Genotype: unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-287(m244) IV. Description: Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc and DpyLet. The DpyLets are sterile adults. Maintain by picking semi-Dpy. Mutagen: EMS Outcrossed: x Made by: Rogalski T Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR692 Species: Caenorhabditis elegans Genotype: unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-275(m245) IV. Description: Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc and DpyLets. Lethal mid-larval. Maintain by picking semi-Dpy. Mutagen: EMS Outcrossed: x Made by: Rogalski T Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR694 Species: Caenorhabditis elegans Genotype: unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-281(m247) IV. Description: Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc and DpyLet. The DpyLets are adult steriles. Maintain by picking semi-Dpy. Mutagen: EMS Outcrossed: x Made by: Rogalski T Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR695 Species: Caenorhabditis elegans Genotype: unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-285(m248) IV. Description: Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc and DpyLet. The DpyLet are adult steriles. Maintain by picking semi-Dpy. Mutagen: EMS Outcrossed: x Made by: Rogalski T Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR697 Species: Caenorhabditis elegans Genotype: cha-1(m324) dpy-13(e184) ama-1(m118)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul and Lethals. Mutagen: Outcrossed: x Made by: Rogalski T Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: DR699 Species: Caenorhabditis elegans Genotype: dpy-13(e184) ama-1(m118m252)/nT1 IV; +/nT1 V. Description: Heterozygotes are Dpy and segregate Dpy, Vul and dead eggs. Homozygous dpy-13 ama-1 animals are embryonic lethals. Strain is sensitive to alpha-amanitin. Mutagen: EMS Outcrossed: x Made by: Rogalski T Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR7 Species: Caenorhabditis elegans Genotype: unc-33(m7) IV; mut-1(e1396). Description: Unc. Mutagen: Spontaneous Outcrossed: x Made by: Riddle D Received: 01/01/76 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR703 Species: Caenorhabditis elegans Genotype: unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-274(m256) IV. Description: Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc, and DpyLets. Lethal early larval. Maintain by picking semi-Dpy. Mutagen: EMS Outcrossed: x Made by: Rogalski T Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR705 Species: Caenorhabditis elegans Genotype: unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-282(m258) IV. Description: Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc and DpyLet. Lethal early larval. Maintain by picking semi-Dpy. Mutagen: EMS Outcrossed: x Made by: Rogalski T Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: DR706 Species: Caenorhabditis elegans Genotype: unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-280(m259) IV. Description: Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc and DpyLets. The DpyLets are adult steriles. Maintain by picking semi-Dpy. Mutagen: EMS Outcrossed: x Made by: Rogalski T Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: DR709 Species: Caenorhabditis elegans Genotype: unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-279(m261) IV. Description: Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc and DpyLets. The DpyLets are adult steriles. Maintain by picking semi-Dpy. Mutagen: EMS Outcrossed: x Made by: Rogalski T Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: DR710 Species: Caenorhabditis elegans Genotype: unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-277(m262) IV. Description: Heterozygotes are semi-Dpy and segregate semi-Dpys, Uncs and Lets. Lethal mid-larval. Maintain by picking semi-Dpy. Mutagen: EMS Outcrossed: x Made by: Rogalski T Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: DR711 Species: Caenorhabditis elegans Genotype: unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-273(m263) IV. Description: Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc and DpyLet. Lethal early larval. Maintain by picking semi-Dpy. Mutagen: EMS Outcrossed: x Made by: Rogalski T Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: DR715 Species: Caenorhabditis elegans Genotype: unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-284(m267) IV. Description: Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc and DpyLet. Lethal early larval. Maintain by picking semi-Dpy. Mutagen: EMS Outcrossed: x Made by: Rogalski T Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: DR717 Species: Caenorhabditis elegans Genotype: unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-286(m269) IV. Description: Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc and DpyLet. The DpyLet are adults which lay eggs that do not hatch. Maintain by picking semi-Dpy. Mutagen: EMS Outcrossed: x Made by: Rogalski T Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: DR72 Species: Caenorhabditis elegans Genotype: daf-4(m72) III. Description: Temperature sensitive dauer constitutive. Small. Amber. Maintain at 15C. Mutagen: EMS Outcrossed: x Made by: MacMorris M Received: 07/01/78 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR721 Species: Caenorhabditis elegans Genotype: lin-4(e912) II. Description: Completely vulvaless. Long. Uncoordinated. Somewhat clear. Mutagen: Outcrossed: x Made by: Edgley M Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR722 Species: Caenorhabditis elegans Genotype: age-1(m333)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: WT phenotype, segregates WT, Egl (age-1 homozygotes) and DpyUncs. Egl animals give all non-recovering dauer larvae (m333 shows maternal effect), with variable radial shrinkage and variable resistance to 1% SDS. Pick WT to maintain and check for correction segregation of progeny. age-1(m333) pka daf-23(m333). Mutagen: EMS Outcrossed: x1 Made by: H. Blagdon/P. Albert Received: 01/06/97 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR730 Species: Caenorhabditis elegans Genotype: dpy-13(e184) ama-1(m118m238) IV. Description: Dpy. Sensitive to alpha-amanitin. Temperature sensitive, maintain at 15C. Fertile at 20C. Emb at restricitve temperature (25C). Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416. Mutagen: EMS Outcrossed: x0 Made by: Teresa Rogalski Received: 11/01/11 Emory University, Atlanta, GA ----------------------------------------------------------------------------- Strain: DR731 Species: Caenorhabditis elegans Genotype: dpy-13(e184) ama-1(m118m251) IV. Description: Dpy. Sensitive to alpha-amanitin. Temperature sensitive, maintain at 15C. Fertile at 20C. Emb at restrictive temperature (25C). Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416. Mutagen: EMS Outcrossed: x0 Made by: Teresa Rogalski Received: 11/01/11 Emory University, Atlanta, GA ----------------------------------------------------------------------------- Strain: DR732 Species: Caenorhabditis elegans Genotype: daf-15(m81) unc-22(s7)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, Twitchers which are dauers (dauer constitutive) and dead eggs. Maintain by picking WT. Hets twitch in 1% nicotine. Mutagen: Outcrossed: x Made by: Albert P Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR733 Species: Caenorhabditis elegans Genotype: lon-2(e678) daf-9(e1406)/unc-78(e1217) X. Description: Balanced lethal dauer constitutive. Heterozygotes are WT and segregate WT, LonDaf and Unc. Will get 3-5 adult Lon per clone. Maintain by picking WT. Mutagen: Outcrossed: x Made by: Albert P Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR767 Species: Caenorhabditis elegans Genotype: unc-17(e113) unc-5(e53)/dpy-13(e184) ama-1(m118) let-288(m306) IV. Description: Heterozygotes are semi-Dpy and segregate semi-Dpy, Unc, and DpyLets. DpyLets are adult steriles. Maintain by picking semi-Dpy. Mutagen: Gamma Rays Outcrossed: x Made by: Rogalski T Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR77 Species: Caenorhabditis elegans Genotype: daf-14(m77) IV. Description: Temperature sensitive dauer constitutive. Tight at 25C. Leaky at 15C. Chemotaxis normal. Mutagen: EMS Outcrossed: x Made by: Albert P Received: 07/01/78 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR786 Species: Caenorhabditis elegans Genotype: ama-1(m322) IV. Description: WT strain. Resistant to _-amanitin. Mutagen: EMS Outcrossed: x2 Made by: Bullerjahn A Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR787 Species: Caenorhabditis elegans Genotype: dpy-13(e184) ama-1(m118) let-276(m240)/nT1 IV; +/nT1 V. Description: Heterozygotes are semi-Dpy and segregate semi-Dpy, Vul, DpyLet and dead eggs. Lethal early larval. Maintain by picking semi-Dpy. Mutagen: Outcrossed: x Made by: Bullerjahn A Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR789 Species: Caenorhabditis elegans Genotype: dpy-13(e184) IV/nT1 [let-?(m435)] (IV;V); unc-42(e270) V/nT1 [let-?(m435)] (IV;V). Description: Heterozygotes are WT and segregate WT, DpyUnc and dead eggs. Maintain by picking WT. Mutagen: Outcrossed: x Made by: Rogalski T Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR792 Species: Caenorhabditis elegans Genotype: sDf2/nT1 IV; +/nT1 V. Description: Hets are WT and segregate WT, dead eggs and Vul (nT1/nT1). Clone WT to maintain. Well balanced. The nT1 homozygotes may overtake the plate at 15C or upon starvation. Mutagen: Outcrossed: x Made by: Rogalski T Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR793 Species: Caenorhabditis elegans Genotype: dpy-13(e184) mDf7 IV/nT1 [let-?(m435)] (IV;V). Description: Heterozygotes are semi-Dpy and segregate semi-Dpy and dead eggs (mDf7/mDf7, nT1/nT1 and aneuploids). This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to Don Riddle. Rec'd new stock 11/99 from Riddle lab. Mutagen: Gamma Rays Outcrossed: x Made by: Rogalski T Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR795 Species: Caenorhabditis elegans Genotype: dpy-13(e184) ama-1(m118) let-276(m239)/nT1 IV; +/nT1 V. Description: Heterozygotes are semi-Dpy and segregate semi-Dpy, Vul, and dead eggs. Homozygous let-276 are dead eggs. Maintain by picking semi-Dpy. Mutagen: EMS Outcrossed: x Made by: Bullerjahn A Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR799 Species: Caenorhabditis elegans Genotype: mDf4/nT1 IV; +/nT1 V. Description: Heterozygotes are semi-Dpy and segregate semi-Dpy, Vul and dead eggs. Maintain by picking semi-Dpy. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to Don Riddle. Mutagen: Gamma Rays Outcrossed: x Made by: Rogalski T Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR802 Species: Caenorhabditis elegans Genotype: mDf5/nT1 IV; +/nT1 V. Description: Heterozygotes are semi-Dpy and segregate semi-Dpy, Vul and dead eggs. Maintain by picking semi-Dpy. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to Don Riddle. Mutagen: Gamma Rays Outcrossed: x Made by: Rogalski T Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR803 Species: Caenorhabditis elegans Genotype: mDf6/nT1 IV; +/nT1 V. Description: Heterozygotes are semi-Dpy and segregate semi-Dpy, Vul and dead eggs. Maintain by picking semi-Dpy. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to Don Riddle. Rec'd new stock 12/99 from Riddle lab. Mutagen: Gamma Rays Outcrossed: x Made by: Rogalski T Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR806 Species: Caenorhabditis elegans Genotype: dpy-13(e184) ama-1(m118m328)/nT1 IV; +/nT1 V. Description: Heterozygotes are WT and segregate WT, Vul, DpyLet and dead eggs. Lethal early larval (L1) at 20C and 25C. Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Minars/Rogalski Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR811 Species: Caenorhabditis elegans Genotype: dpy-13(e184) ama-1(m118m332) IV/nT1 [qIs51] (IV;V). Description: Sensitive to alpha-amanitin. Heterozygotes are semi-Dpy and segregate semi-Dpy, Vul, dead embryos, and Dpy. Dpy progeny arrest in L1 stage at >20C. Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416.Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416. Mutagen: EMS Outcrossed: x0 Made by: Teresa Rogalski Received: 11/01/11 Emory University, Atlanta, GA ----------------------------------------------------------------------------- Strain: DR814 Species: Caenorhabditis elegans Genotype: dpy-13(e184) ama-1(m118) mDf8 IV/nT1 [let-?(m435)] (IV;V). Description: Heterozygotes are semi-Dpy and segregate semi-Dpy, early larval lethals and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to Don Riddle. Mutagen: Gamma Rays Outcrossed: x Made by: Rogalski T Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR842 Species: Caenorhabditis elegans Genotype: mut-4(st700) I; mut-5(st701) II; unc-22(st136m498) IV. Description: Non-Twitchers. Still contains Tc1. Temperature sensitive. Sterile at 25C, grows ok at 20C. Dauer larvae are SDS resistant. Mutagen: Tc1/Spontaneous Outcrossed: x Made by: Albert P Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR848 Species: Caenorhabditis elegans Genotype: daf-8(e1393) unc-29(e1072) I. Description: Unc-weak kinker, doesn't back well. Temperature sensitive dauer constitutive. Maintain at 15C. Makes high percentage of dauers at 25C. tsp for dauer formation is L1 molt. Type C Egl. Active. Resistant to 1 mM levamisole. Sensitive to hypoosmotic shock. Mutagen: Outcrossed: x Made by: Edgley M Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR86 Species: Caenorhabditis elegans Genotype: daf-19(m86) II. Description: Temperature sensitive dauer constitutive. Difficult to grow. 100% dauers at 25C. 90% dauers at 15C. Mutagen: EMS Outcrossed: x Made by: Riddle D Received: 09/01/78 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR877 Species: Caenorhabditis elegans Genotype: dpy-13(e184) ama-1(m118m367) IV/nT1 [qIs51] (IV;V). Description: Sensitive to alpha-amanitin. Heterozygotes are semi-Dpy and segregate semi-Dpy, Vul, dead embryos, and Dpy. Dpy progeny arrest in L1 stage at >20C. Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416.Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416. Mutagen: EMS Outcrossed: x0 Made by: Teresa Rogalski Received: 11/01/11 Emory University, Atlanta, GA ----------------------------------------------------------------------------- Strain: DR880 Species: Caenorhabditis elegans Genotype: dpy-13(e184) ama-1(m118m370) IV/nT1 [qIs51] (IV;V). Description: Sensitive to alpha-amanitin. Heterozygotes are semi-Dpy and segregate semi-Dpy, Vul, dead embryos, and Dpy. Dpy progeny arrest in L1 stage at >20C. Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416.Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416. Mutagen: EMS Outcrossed: x0 Made by: Teresa Rogalski Received: 11/01/11 Emory University, Atlanta, GA ----------------------------------------------------------------------------- Strain: DR892 Species: Caenorhabditis elegans Genotype: dpy-13(e184) ama-1(m118m396) IV/nT1 [qIs51] (IV;V). Description: Temperature sensitive. Sensitive to alpha-amanitin. Heterozygotes are semi-Dpy and segregate semi-Dpy, Vul, dead embryos, and Dpy. Dpy progeny are maternal effect Emb at 20C and arrest in mid-larval stages at 25C. Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416.Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416. Mutagen: EMS Outcrossed: x0 Made by: Teresa Rogalski Received: 11/01/11 Emory University, Atlanta, GA ----------------------------------------------------------------------------- Strain: DR907 Species: Caenorhabditis elegans Genotype: unc-17(e113) dpy-13(e184) IV; mDp1 (IV;f). Description: Animals which carry the Dup are semi-Dpy and segregate semi-Dpy and DpyUnc (animals which have lost the Dup). Animals homozygous for the Dup are slow growing, slender and transparent. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to Don Riddle. Mutagen: Gamma Rays Outcrossed: x Made by: Rogalski T Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR918 Species: Caenorhabditis elegans Genotype: mDf10/nT1 IV; +/nT1 V. Description: Heterozygotes are semi-Dpy and segregate semi-Dpy, Vul, early larval lethals and dead eggs. Maintain by picking semi-Dpy. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to Don Riddle. Mutagen: Gamma Rays Outcrossed: x Made by: Bullerjahn A Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR921 Species: Caenorhabditis elegans Genotype: dpy-7(sc27) daf-9(e1406)/lon-2(e678) X. Description: Heterozygotes are WT. At 25C the hets segregate DpyDafs, WT and Lon. At 15C the hets segregate Dafs, WT and Lon. [dpy-7(sc27) is temperature sensitive. At 25C it is larger than most Dpys and has a tendency to roll. Not expressed at 15C.] Maintain by picking WT. Mutagen: EMS Outcrossed: x Made by: Albert P Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR927 Species: Caenorhabditis elegans Genotype: daf-8(e1393) fer-1(hc1) I. Description: Temperature sensitive dauer constitutive. Temperature sensitive fertilization defective. WT at 15C, segregates some dauers at 20C, sterile at 25C. Tightly linked. Mutagen: Outcrossed: x Made by: Edgley M Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR933 Species: Caenorhabditis elegans Genotype: dpy-13(m399) IV. Description: Dpy. Mutagen: Outcrossed: x6 Made by: Albert P Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR94 Species: Caenorhabditis elegans Genotype: unc-45(m94) III. Description: Temperature sensitive Unc. Slow at 15C. Paralyzed at 20C and 25C. Mutagen: EMS Outcrossed: x Made by: Brown S Received: 04/01/80 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR942 Species: Caenorhabditis elegans Genotype: let-278(m265) dpy-13(e184) ama-1(m118)/nT1 IV; +/nT1 V. Description: Heterozygotes are semi-Dpy and segregate semi-Dpy, Vul, DpyLet and dead eggs. The DpyLets are Sterile adults. Maintain by picking semi-Dpy. Mutagen: EMS Outcrossed: x Made by: Rogalski T Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR943 Species: Caenorhabditis elegans Genotype: daf-8(e1393) fer-1(hc1) unc-29(e1072) I. Description: Unc. Temperature sensitive dauer constitutive. Temperature sensitive fertilization defective (late). UncDaf at 25C, Unc at 15C. Mutagen: Outcrossed: x Made by: Edgley M Received: 03/15/25 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR96 Species: Caenorhabditis elegans Genotype: unc-76(e911) V. Description: Coiler Unc. Recessive. Easily scored. Mutagen: P32 Outcrossed: x Made by: MacMorris M Received: 01/01/78 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR960 Species: Caenorhabditis elegans Genotype: daf-1(m402) IV. Description: Recessive maternal dauer constitutive. Maintain at 15C Mutagen: Outcrossed: x2 Made by: Albert P Received: 03/15/25 Children's Hospital ----------------------------------------------------------------------------- Strain: DR966 Species: Caenorhabditis elegans Genotype: dpy-13(e184) ama-1(+) IV. Description: Dpy. Sensitive to alpha-amanitin. [NOTE: formerly described as ama-1(m118m370m414), it has been determined that m414 was reversion of m367 back to wild-type sequence.] Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416. Mutagen: EMS Outcrossed: x0 Made by: Teresa Rogalski Received: 11/01/11 Emory University, Atlanta, GA ----------------------------------------------------------------------------- Strain: DR97 Species: Caenorhabditis elegans Genotype: unc-26(e345) IV. Description: Unc. Mutagen: Heat shock Outcrossed: x Made by: Riddle D Received: 10/01/75 Genome BC, Vancouver, BC, Canada ----------------------------------------------------------------------------- Strain: DR976 Species: Caenorhabditis elegans Genotype: dpy-13(e184) ama-1(m118m370m417) IV. Description: Dpy. Sensitive to alpha-amanitin. Reference: Bowman EA, Riddle DR, Kelly WG. G3 November 2011 1:411-416. Mutagen: EMS Outcrossed: x0 Made by: Teresa Rogalski Received: 11/01/11 Emory University, Atlanta, GA ----------------------------------------------------------------------------- Strain: DR978 Species: Caenorhabditis elegans Genotype: daf-12(m419) X. Description: daf-d. Weak heterochronic phenotypes in seam, somatic gonad, and intestine. Class III allele. Mutagen: EMS Outcrossed: x Made by: Don Riddle Received: 11/03/00 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: DR979 Species: Caenorhabditis elegans Genotype: daf-12(m420) X. Description: daf-d. Weak heterochronic phenotypes in intestine. Class III allele. Mutagen: EMS Outcrossed: x Made by: Don Riddle Received: 10/23/00 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: DR980 Species: Caenorhabditis elegans Genotype: daf-12(m421) X. Description: daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele. Mutagen: Outcrossed: x Made by: Don Riddle Received: 10/23/00 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: DR981 Species: Caenorhabditis elegans Genotype: daf-12(m422) X. Description: daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele. Mutagen: EMS Outcrossed: x Made by: Don Riddle Received: 10/23/00 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: DR983 Species: Caenorhabditis elegans Genotype: daf-12(m424) X. Description: daf-d. Weak heterochronic phenotypes in intestine. Class III allele. Mutagen: EMS Outcrossed: x Made by: Don Riddle Received: 11/03/00 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: DS72 Species: Caenorhabditis elegans Genotype: mat-3(ax68) III; him-8(e1489) IV. Description: Temperature sensitive. Maintain at 15C. Mutagen: EMS Outcrossed: x>3 Made by: Matt Wallenfang Received: 03/01/01 College of William and Mary, Williamsburg, VA ----------------------------------------------------------------------------- Strain: DS73 Species: Caenorhabditis elegans Genotype: mat-3(or180) III; him-8(e1489) IV. Description: Temperature sensitive. Maintain at 15C. Mutagen: EMS Outcrossed: x>3 Made by: Danielle Hamill Received: 03/01/01 College of William and Mary, Williamsburg, VA ----------------------------------------------------------------------------- Strain: DS77 Species: Caenorhabditis elegans Genotype: mat-1(ax161) I; him-8(e1489) IV. Description: Temperature sensitive. Maintain at 15C. Mutagen: EMS Outcrossed: x>3 Made by: Matt Wallenfang Received: 03/01/01 College of William and Mary, Williamsburg, VA ----------------------------------------------------------------------------- Strain: DS80 Species: Caenorhabditis elegans Genotype: mat-1(ax144) I; him-8(e1489) IV. Description: Temperature sensitive. Maintain at 15C. Mutagen: EMS Outcrossed: x>3 Made by: Matt Wallenfang Received: 03/01/01 College of William and Mary, Williamsburg, VA ----------------------------------------------------------------------------- Strain: DS88 Species: Caenorhabditis elegans Genotype: emb-27(ax81) II; him-5(e1490) V. Description: Temperature sensitive. Maintain at 15C. Mutagen: EMS Outcrossed: x>3 Made by: Matt Wallenfang Received: 03/01/01 College of William and Mary, Williamsburg, VA ----------------------------------------------------------------------------- Strain: DS97 Species: Caenorhabditis elegans Genotype: apc-1(ax76) II. Description: Temperature sensitive. Maintain at 15C. Formerly known as mat-2. Mutagen: EMS Outcrossed: x>3 Made by: Matt Wallenfang Received: 03/01/01 College of William and Mary, Williamsburg, VA ----------------------------------------------------------------------------- Strain: DS98 Species: Caenorhabditis elegans Genotype: apc-1(ax102) II. Description: Temperature sensitive. Maintain at 15C. Formerly known as mat-2. Mutagen: EMS Outcrossed: x>3 Made by: Matt Wallenfang Received: 03/01/01 College of William and Mary, Williamsburg, VA ----------------------------------------------------------------------------- Strain: DU23 Species: Caenorhabditis elegans Genotype: spe-15(ok153) dpy-5(e61) I; him-8(e1489) IV; sDp2 (I;f). Description: Animals with the duplication are WT. Animals which have lost the duplication are Dpy and Sterile. Throws males. Mutagen: UV/TMP Outcrossed: x8 Made by: Kelleher/Titus Received: 02/04/04 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DUP223 Species: C. elegans Genotype: glh-1(sam129[glh-1::T2A::sGFP2(1-10)]) I. Description: T2A::sGFP2(1-10) fused to the C-terminus of endogenous GLH-1. The T2A self-cleaving peptide separates sGFP2(1-10) from GLH-1 post-translationally so that sGFP2(1-10) disperses throughout germ cell nuclei and cytoplasm. sGFP2(1-10) is also maternally loaded into embryos, where it persists through early and mid-embryonic development. Proteins tagged with the 16aa GFP11 or M3 sequence will bind sGFP2(1-10) in the germline and early embryo to emit GFP fluorescence. Broods from DUP223 are similar to wild-type at permissive and restrictive temperatures. Transgene tag was inserted by CRISPR/Cas9 in an N2 background. [NOTE: (04/23/2021) The original stock received by the CGC was found to be carrying a second insertion, clu-1(sam131[GFP(11)::clu-1]. A new stock verified by to be carrying the correct transgene was received from DUP 05/04/2021.] Reference: Goudeau J, et al. Genetics. 2021 Apr; 217(4): iyab014. PMID: 33693628. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Dustin L Updike Received: 05/04/21 Mt Desert Island Bio Lab, Salisbury Cove, ME ----------------------------------------------------------------------------- Strain: DUP237 Species: C. elegans Genotype: glh-1(sam140[glh-1::T2A::wrmScarlet(1-10)]) I. Description: T2A::wrmScarlet(1-10) fused to the C-terminus of endogenous GLH-1. The T2A self-cleaving peptide separates wrmScarlet(1-10) from GLH-1 post-translationally so that wrmScarlet(1-10) disperses throughout germ cell nuclei and cytoplasm. wrmScarlet(1-10) is also maternally loaded into embryos, where it persists through early and mid-embryonic development. Proteins tagged with the 18aa wrmScarlet(11) sequence will bind wrmScarlet(1-10) in the germline and early embryo to emit wrmScarlet fluorescence. Broods from DUP237 are similar to wild-type at permissive and restrictive temperatures. Transgene tag was inserted by CRISPR/Cas9 in an N2 background. Reference: Goudeau J, et al. Genetics. 2021 Apr; 217(4): iyab014. PMID: 33693628. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Dustin L Updike Received: 12/09/20 Mt Desert Island Bio Lab, Salisbury Cove, ME ----------------------------------------------------------------------------- Strain: DV2208 Species: Caenorhabditis elegans Genotype: unc-97(su110) X. Description: Made by outcrossing HE110 four times. su110 is moderately Smg suppressible: HE110 animals are significantly less Unc than DV2208, and HE110 animal are also pVul, which is characteristic of Smg mutations. Mutagen: EMS Outcrossed: x4 Made by: Dave Reiner Received: 03/07/07 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: DV2275 Species: Caenorhabditis elegans Genotype: daf-2(e1370) dpy-17(e164) III. Description: Mutagen: Outcrossed: x Made by: Dave Reiner Received: 02/22/08 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: DV2689 Species: Caenorhabditis elegans Genotype: sec-5(pk2357)/mIn1 [dpy-10(e128) mIs14] II. Description: Heterozygotes segregate wild-type GFP+ heterozygotes, GFP+ Dpy, and sec-5 homozygotes (scrawny, small broods, abnormal gut appearance) sec-5 is homozygous maternal-effect lethal; M+Z- animals produce a few dead L1-L2 stage larvae with Vab defects. Pick GFP+ wild-type to maintain. Based upon phenotype, pk2357 is a strong loss-of-function, but likely not a null allele; molecular lesion produces a premature stop at position 389. Reference: Frische EW, et al. EMBO J. 2007 Dec 12;26(24):5083-92. [NOTE: This strain was previously described as carrying pk2358, but pk2357 is the correct allele. Both pk2357 and pk2358 cause the same nonsense (amber) change.] Mutagen: Outcrossed: x Made by: Dave Reiner Received: 04/16/12 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: DV3089 Species: C. elegans Genotype: rheb-1(re64[mKate2::3xFlag::rheb-1]) III. Description: mKate tag inserted at 5' end of endogenous rheb-1 locus. Ubiquitous expression. Mutagen: CRISPR Outcrossed: x3 Made by: Tam Duong Received: 09/05/19 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: DV3208 Species: C. elegans Genotype: daf-15(re147[daf-15::mNG::2xHA]) IV. Description: mNeonGreen tag inserted at 3' end of endogenous daf-15 locus. Ubiquitous expression. Mutagen: CRISPR Outcrossed: x0 Made by: Tam Duong Received: 09/05/19 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: DV3285 Species: C. elegans Genotype: his-72(cp76[mNeonGreen::3xFlag::his-72]) mpk-1(re172[mpk-1::mKate2::3xFlag]) III. Description: Green nuclei and ubiquitous cytosolic red expression, typically excluded from nuclei but with activity-dependent translocation into nuclei. Derived in an N2 background. C-terminally tagged mpk-1 is detectable by triplex PCR: mpk-1 genotyping FW: ACCAAAACAACCATGGGCTCG mpk-1 genotyping RV-1: GCTCCAAGTATGGGTGAGCC mpk-1 genotyping RV-2: GGTTCCCTCGTATGGCTTTCC Reference: Neal R, et al. (2021). Nuclear translocation of tagged endogenous ERK/MPK-1 MAP Kinase denotes a subset of activation events in C. elegans development. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Neal Rasmussen Received: 05/26/21 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: DV3312 Species: C. elegans Genotype: rgl-1(re179[mNeonGreen::3xFlag::rgl-1]) X. Description: Ubiquitous expression with cytosolic localization. Derived in an N2 background. Detection with triplex primers: HS125 5’-CTTGTCACTGTAAGGGAAGATTTCC-3’ HS126 5’-TTGTCCTCCTCTCCCTTGG-3’ HS127 5’ ACGTAGAATGTTCCAGAGTTCCAG-3' Reference: Shin H, et al. Cell Rep. 2018 Sep 4;24(10):2669-2681. PMID: 30184501 Mutagen: CRSIPR/Cas9 Outcrossed: x0 Made by: Hanna Shin Received: 05/26/21 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: DV3313 Species: Caenorhabditis elegans Genotype: rap-1(re180) IV. Description: Gain-of-function allele (G12V). Low penetrance of 3˚ to 1˚ fate transformations (Muv) and duplication of the excretory duct cell. Genotyping primers (Tm=50C, followed by BamHI digestion): oNR122: TGTGTCATCTGGTCTGTACTTGG; oNR123: TCCCCTGCACGAATTGTACC. Reference: Rasmussen NR, Dickinson DJ, and Reiner DJ. Genetics Dec;210(4):1339-1354. doi: 10.1534/genetics.118.301601. PMID: 30257933. Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Neal Rasmussen Received: 07/06/21 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: DV3327 Species: C. elegans Genotype: pmk-1(re170[pmk-1::mNG::3xFlag]) IV. Description: mNeonGreen and 3xFlag tag inserted at 3' end of endogenous pmk-1 locus. Fluorescent green signal detected in both cytosol and nuclei of all somatic cells; might be silenced in the germ line. Generated in an N2 background. Reference: Shin H, et al. Cell Rep. 2018 Sep 4;24(10):2669-2681. PMID: 30184501 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Hanna Shin Received: 05/16/22 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: DV3402 Species: C. elegans Genotype: ral-1(re218[mKate2::3xFlag::ral-1]) III. Description: Ubiquitous expression and localization to plasma membranes. Derived in an N2 background. TD185 5’ -GCCGGAAGAGTGATGAACCC- 3’ TD186 5’ -TAATGAGCTCGGAGACCATGGC- 3’ TD187 5’ -CGCACCTCATCATACATGAACTGC- 3’ Reference: Shin H, et al. Cell Rep. 2018 Sep 4;24(10):2669-2681. PMID: 30184501 Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Hanna Shin Received: 05/26/21 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: DV3462 Species: C. elegans Genotype: rheb-1(re242)/qC1 [dpy-19(e1259) glp-1(q339)] nIs189 III. Description: nIs189 [myo-2::GFP] integrated in or near qC1. No recombination seen between nIs189 and qC1; fails to complement all markers on qC1. Pick wild-type GFP+ to maintain. Heterozygotes are wild-type GFP+ (pharynx), and segregate wild-type GFP+ heterozygotes, Dpy Sterile GFP+, and non-GFP re242 homozygotes (arrested at L3). Mutagen: CRISPR Outcrossed: x1 Made by: Tam Duong Received: 09/05/19 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: DV3525 Species: C. elegans Genotype: daf-15(re257[daf-15::mNG::AID]) IV. Description: mNeonGreen tag inserted at 3' end of endogenous daf-15 locus; AID at 3' end of mNeonGreen. Ubiquitous expression. Transgene can be degraded in a background expressing TIR1 co-factor and supplemented with auxin. Mutagen: CRISPR Outcrossed: x0 Made by: Tam Duong Received: 09/05/19 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: DV3670 Species: C. elegans Genotype: rheb-1(re64 re285[AID::mKate2::3xFlag::rheb-1]) III. Description: AID tag in 5' end of mKate2-tagged endogenous rheb-1. Ubiquitous red expression. Mutagen: CRISPR Outcrossed: x0 Made by: Tam Duong Received: 09/05/19 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: DV3765 Species: Caenorhabditis elegans Genotype: scd-1(re305[scd-1::mKate2::2xHA]) X. Description: mKate GLO (germline optimized) tag inserted at C-terminus of endogenous SCD-1. Red fluorescence in all nuclei. Cas9 guide + PAM: GACTTGGAAGAAGACGGTGG+AGG. Reference: Ailion M, et al. In preparation. Mutagen: CRSIPR/Cas9 Outcrossed: x0 Made by: Nathan Vickery Received: 05/16/22 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: DV3799 Species: Caenorhabditis elegans Genotype: reSi1 I. Description: reSi1 [col-10p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (I:-5.32). Hypodermal-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in hypodermal nuclei. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x6 Made by: Tam Duong Received: 02/03/20 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: DV3800 Species: Caenorhabditis elegans Genotype: reSi2 II. Description: reSi2 [col-10p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (II:0.77). Hypodermal-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in hypodermal nuclei. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Tam Duong Received: 02/03/20 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: DV3801 Species: Caenorhabditis elegans Genotype: reSi3 I. Description: reSi3 [unc-54p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (I:-5.32). Muscle-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in muscle nuclei. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Tam Duong Received: 02/03/20 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: DV3803 Species: Caenorhabditis elegans Genotype: reSi5 I. Description: reSi5 [ges-1p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (I:-5.32). Intestinal-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in intestinal nuclei. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Tam Duong Received: 02/03/20 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: DV3805 Species: Caenorhabditis elegans Genotype: reSi7 I. Description: reSi7 [rgef-1p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (I:-5.32). Neuronal-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in neuronal nuclei. NOTE: PCR detection of reSi7 insert using the published primers has been reported to be defective. These primers designed by Sherlyn Wijaya and Claire Richardson to detect ttTi4338 (LG I) also work for reIs7: ttTi4338 (LG I) wrdSi23-F: cttcaaagaaatcgccgac wrdSi23-FP: AACAACGAGACCTACGTCG wrdSi23-R: Ctctaagatgtcggccac (wt ~300bp, mutant ~650bp). Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Tam Duong Received: 02/03/20 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: DV3825 Species: Caenorhabditis elegans Genotype: reSi11 II. Description: reSi11 [unc-54p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (II:0.77). Muscle-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in muscle nuclei. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Tam Duong Received: 02/03/20 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: DV3826 Species: Caenorhabditis elegans Genotype: reSi12 II. Description: reSi12 [ges-1p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (II:0.77). Intestinal-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in intestinal nuclei. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Tam Duong Received: 02/03/20 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: DV4326 Species: C. elegans Genotype: bar-1(ga80) X. Description: Variably penetrant Unc, Vul, pVul, Egl, vulval rupture, and slow growth. Parental strain EW15 was outcrossed to separate bar-1(ga80) from a described background PRY-1(N354K) mutation. The phenotypes of the outcrossed strain are much more severe than those of the progenitor strain EW15. Mutagen: Outcrossed: x2 Made by: Snigdha Musugunthan Received: 02/14/25 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: DW101 Species: Caenorhabditis elegans Genotype: atl-1(tm853) V/nT1 [unc-?(n754) let-? qIs50] (IV;V). Description: Heterozygotes are Unc and GFP+ with signal in the pharynx. atl-1(tm853) homozygotes are non-Unc, viable, GFP-, and produce 100% dead embryos. qIs50 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter (F22B7.9) driving GFP in the intestine. nT1[unc-?(n754) let-? qIs50] is also known as DnT1[qIs50]. qIs50 is apparently inserted on DnT1. qIs50 is somewhat dimmer than the similar qIs51. Mutagen: UV/TMP Outcrossed: x7 Made by: Mitani/Garcia-Muse Received: 04/24/06 ICRF Clare Hall, South Mimms, UK ----------------------------------------------------------------------------- Strain: DW102 Species: Caenorhabditis elegans Genotype: brc-1(tm1145) III. Description: Homozygous viable. Weak Him phenotype (2-3%). Extremely sensitive to ionizing radiation and other DNA damaging agents. Mutagen: UVA/TMP Outcrossed: x6 Made by: Shohei Mitani Received: 05/08/06 ICRF Clare Hall, South Mimms, UK ----------------------------------------------------------------------------- Strain: DW103 Species: Caenorhabditis elegans Genotype: brd-1(dw1) III. Description: Homozygous viable. Weak Him phenotype (2-3%). Extremely sensitive to ionizing radiation and other DNA damaging agents. Mutagen: UVA/TMP Outcrossed: x6 Made by: Mark Petalcorin Received: 05/08/06 ICRF Clare Hall, South Mimms, UK ----------------------------------------------------------------------------- Strain: DW104 Species: Caenorhabditis elegans Genotype: brc-2(tm1086) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: tm1086 is homozygous lethal. Maternally rescued. Fails to produce viable progeny due to a defect in repairing meiotic DNA double-strand breaks. Chromosomes are visibly aggregated at diakinesis. Maintain by picking GFP progeny and checking that the non-GFP progeny that are produced fail to give viable progeny. qIs48 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter driving GFP in the intestine, and is homozygous lethal. Mutagen: UVA/TMP Outcrossed: x6 Made by: Shohei Mitani Received: 05/08/06 ICRF Clare Hall, South Mimms, UK ----------------------------------------------------------------------------- Strain: DWF1105 Species: Acrobeloides sp. Genotype: Acrobeloides sp. wild isolate. Description: Isolated in Arizona desert. Identification by E. Mae Noffsinger. Mutagen: Outcrossed: x Made by: Received: 02/15/96 Colorado State University, Fort Collins, CO ----------------------------------------------------------------------------- Strain: DWF1106 Species: Acrobeloides sp. Genotype: Acrobeloides sp. wild isolate. Description: Isolated in Arizona desert. Identification by E. Mae Noffsinger as Acrobeloides obliquus. Mutagen: Outcrossed: x Made by: Received: 02/15/96 Colorado State University, Fort Collins, CO ----------------------------------------------------------------------------- Strain: DWF1107 Species: Acrobeloides butschlii Genotype: Acrobeloides butschlii wild isolate. Description: Original sample from peach orchard at Lodi CA. Original culture sent to DWF from B. Jaffee. Identification by E. Mae Noffsinger. Mutagen: Outcrossed: x Made by: Received: 02/15/96 Colorado State University, Fort Collins, CO ----------------------------------------------------------------------------- Strain: DWF1108 Species: Acrobeloides sp. Genotype: Acrobeloides sp. wild isolate. Description: Originally from ecological study at University of GA. Identification by E. Mae Noffsinger. Mutagen: Outcrossed: x Made by: Received: 02/15/96 Colorado State University, Fort Collins, CO ----------------------------------------------------------------------------- Strain: DWF1109 Species: Acrobeloides thornei Genotype: Acrobeloides thornei wild isolate. Description: Mentioned in Soil Biol. Biochem 25(9): 1141-1151, 1993. Sent to DWF from E.M. Noffsinger. [6/98: Paul De Ley has checked this strain and suggests that it doesn't match the original description of Acrobeloides thornei very well.] Mutagen: Outcrossed: x Made by: Received: 02/15/96 Colorado State University, Fort Collins, CO ----------------------------------------------------------------------------- Strain: DWF1110 Species: Acrobeloides ellesmerensis Genotype: Acrobeloides ellesmerensis wild isolate. Description: Mentioned in Soil Biol. Biochem 25(9): 1141-1151, 1993. Sent to DWF from E.M. Noffsinger. Isolated in CA. Mutagen: Outcrossed: x Made by: Received: 02/15/96 Colorado State University, Fort Collins, CO ----------------------------------------------------------------------------- Strain: DWF1301 Species: Cephalobus sp. Genotype: Cephalobus sp. wild isolate. Description: Isolated in Fort Collins, CO. Species identification done by Lynn Carta. Mutagen: Outcrossed: x Made by: Received: 02/15/96 Colorado State University, Fort Collins, CO ----------------------------------------------------------------------------- Strain: DWF1501 Species: Dolichorhabditis sp. Genotype: Dolichorhabditis sp. wild isolate. Description: Isolated in Brazil. Mutagen: Outcrossed: x Made by: Received: 02/15/96 Colorado State University, Fort Collins, CO ----------------------------------------------------------------------------- Strain: DWF1604 Species: Operculrhabditis sp. Genotype: Operculrhabditis sp. wild isolate Description: Originally from UCR collection. Identification by E. Mae Noffsinger. Mutagen: Outcrossed: x Made by: Received: 02/15/96 Colorado State University, Fort Collins, CO ----------------------------------------------------------------------------- Strain: DWF1701 Species: Zeldia sp. Genotype: Zeldia sp. wild isolate. Description: Isolated in Fort Collins, CO. Species identification done by Lynn Carta. Mutagen: Outcrossed: x Made by: Received: 02/15/96 Colorado State University, Fort Collins, CO ----------------------------------------------------------------------------- Strain: DWP219 Species: Caenorhabditis elegans Genotype: daam-1(ups39) V. Description: Superficially wild-type. ups39 is a CRISPR-engineered deletion within daam-1. daam-1(ups39) encodes an in-frame stop codon near the start of its FH2-coding sequence, and a 1-nt frame shift due to the LoxP site, and is thus predicted encode a non-functional formin. Reference: Sundaramurthy S, et al. Cytoskeleton (Hoboken). 2020 Oct;77(10):422-441. doi: 10.1002/cm.21639. PMID: 33103378. Mutagen: CRISPR-Cas9 Outcrossed: x6 Made by: David Pruyne Received: 04/07/23 SUNY-UMU, Syracuse, NY ----------------------------------------------------------------------------- Strain: DWP294 Species: C. elegans Genotype: rhIs2. Description: rhIs2 [pat-3::HA::GFP]. rhIs2 contains cosmid-derived full-length pat-3, including 5 kb 5’UTR and 1 kb 3’ UTR, with HA and GFP(S65C) tags inserted prior to the pat-3 stop codon. Reference: Plenefisch JD, et al. Development. 2000 127(6):1197-207. doi: 10.1242/dev.127.6.1197. Mutagen: Outcrossed: x0 Made by: John Plenefisch Received: 04/07/23 SUNY-UMU, Syracuse, NY ----------------------------------------------------------------------------- Strain: DWP3 Species: C. elegans Genotype: qaIs8001. Description: qaIs8001 [fhod-1::GFP + unc-119(+)]. Integrated functional translational FHOD-1::GFP fusion. Superficially wild-type. Reference: Mi-Mi L, et al. J Cell Biol. 2012 Jul 9;198(1):87-102. doi: 10.1083/jcb.201202053. Mutagen: Outcrossed: x7 Made by: David Pruyne Received: 04/07/23 SUNY-UMU, Syracuse, NY ----------------------------------------------------------------------------- Strain: DY164 Species: Caenorhabditis elegans Genotype: unc-32(e189) syIs80 III; him-8(e1489) IV. Description: syIs80 [(pPGF11.13) lin-11::GFP + unc-119(+)] III. Animals are Unc. GFP fluorescence is observed in vulval cells, uterine pi cells and VC neurons. Mutagen: Outcrossed: x Made by: Received: 04/07/08 McMaster University, Hamilton, Canada ----------------------------------------------------------------------------- Strain: DY31 Species: Caenorhabditis briggsae Genotype: Cbr-lev-?(sy5440) I. Description: Mutagen: Outcrossed: x Made by: Received: 02/28/06 McMaster University, Hamilton, Canada ----------------------------------------------------------------------------- Strain: DZ205 Species: Caenorhabditis elegans Genotype: dsh-2(ez25)/mIn1 [mIs14 dpy-10(e128)] II; him-8(e1489) IV. Description: mIs14 [myo-2p::GFP + pes-10p::GFP]. Him. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ heterozygotes, Dpy bright GFP+ (mIn1 homozygotes), and Egl non-GFP ez25 homozygotes. Pick WT dim GFP and check for correct segregation of progeny to maintain. Mutagen: EMS Outcrossed: x2 Made by: Weiru Chang Received: 09/28/06 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DZ224 Species: Caenorhabditis elegans Genotype: him-8(e1489) IV; ezIs1 X. Description: ezIs1[K09C8.2::GFP + rol-6(su1006)]. ezIs1 was integrated with pPD95.65(K09C8.2 promoter and unc-54 3') and pRF4. Worms are 100% Rollers. GFP is expressed in male seminal vesicle and vas deferens cells. No expression in the hermaphrodite gonad is observed. Mutagen: Gamma Rays Outcrossed: x4 Made by: K Thoemke & W Chang Received: 05/26/04 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DZ240 Species: Caenorhabditis elegans Genotype: fkh-6(ez16)/mIn1 [dpy-10(e128) mIs14] II; him-8(e1489) IV. Description: Heterozygotes are WT and GFP+ in the pharynx. mIn1[dpy-10(e128) mIs14] homozygotes are Dpy and GFP+ in the pharynx. Homozygous fkh-6(ez16) hermaphrodites are sterile and have gonadogenesis defects. Homozygous fkh-6(ez16) males are sterile and strong Gon, "white patch" phenotype. 25% of males have a hermaphrodite vulval structure. Mutagen: EMS Outcrossed: x6 Made by: Weiru Chang Received: 05/19/04 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DZ325 Species: Caenorhabditis elegans Genotype: ezIs2 III; him-8(e1489) IV. Description: ezIs2 [fkh-6::GFP + unc-119(+)]. ezIs2 was integrated with pPD95.69 (fkh-6 promoter and unc-54 3') and pMM106b (unc-119(+)). Worms are 100% non-Unc (the unc-119 background has been crossed out). GFP expression in adult hermaphrodite spermatheca is bright and weak staining is also observed in the proximal sheath cells. Weak GFP staining is also observed in Z1/Z4 cells in both sexes. Mutagen: Gamma Rays Outcrossed: x6 Made by: Weiru Chang Received: 05/26/04 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DZ390 Species: Caenorhabditis elegans Genotype: ezIs10 II; unc-119(ed3) III; him-8(e1489) IV. Description: ezIs10 [(pWY3) lin-32::GFP + unc-119(+)]. Mutagen: Outcrossed: x Made by: J. Ross Received: 03/27/08 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DZ393 Species: Caenorhabditis elegans Genotype: mab-3(e1240) unc-4(e120) ref-1(ez11) II; him-8(e1489) IV. Description: Unc (cannot back). WT male tail. Mutagen: EMS Outcrossed: x5 Made by: J. Ross Wolff Received: 09/28/06 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DZ683 Species: Caenorhabditis elegans Genotype: tra-2(ar221) II; xol-1(y9) X; rdIs4. Description: rdIs4 [ehn-3a::Venus(delta)]. Temperature-sensitive. Must be maintained at 15°C to produce progeny. Strain develops as hermaphrodites at 15°C (some animals are intersex with male tails), and develops as XX pseudomales at 25°C. GFP expressed in gonadal precursors. Reference: Kroetz MB & Zarkower D. G3 (Bethesda). 2015 Oct 23;5(12):2831-41. Mutagen: Outcrossed: x0 Made by: Mary Kroetz Received: 07/20/16 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DZ685 Species: Caenorhabditis elegans Genotype: xol-1(y9) X; rdIs4. Description: rdIs4 [ehn-3a::Venus(delta)]. Slightly Egl. Male lethal. GFP expressed in gonadal precursors. Reference: Kroetz MB & Zarkower D. G3 (Bethesda). 2015 Oct 23;5(12):2831-41. Mutagen: Outcrossed: x0 Made by: Mary Kroetz Received: 07/20/16 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DZ710 Species: Caenorhabditis elegans Genotype: fkh-6(ez73[3xflag + Cbr-unc-119(+)]) II. Description: fkh-6(ez73[3xflag + Cbr-unc-119(+)]) II. Mutagen: Outcrossed: x5 Made by: Mary Kroetz Received: 07/20/16 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DZ840 Species: Caenorhabditis elegans Genotype: tra-1(ez72[biotag::GFP::TEV::3xflag::tra-1]) III. Description: tra-1(ez72[biotag::GFP::TEV::3xflag::tra-1]) III. Mutagen: Outcrossed: x0 Made by: Matt Berkseth Received: 07/20/16 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: DZ841 Species: Caenorhabditis elegans Genotype: tra-1(ez72[biotag::GFP::TEV::3xflag::tra-1]) III; zuIs236. Description: tra-1(ez72[biotag::GFP::TEV::3xflag::tra-1]) III. zuIs236 [his-72(1 kb 5'UTR)::BIRA::GFP::his-72(1 kb 3'UTR) + unc-119(+)]. Location of zuIs236 is not known, but is not in LG III. Mutagen: Outcrossed: x0 Made by: Matt Berkseth Received: 07/20/16 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: EA81 Species: Caenorhabditis elegans Genotype: pag-3(ls20) X. Description: Recessive. Misexpresses mec-7 in the BDU neurons. Unc. Males can mate at 15C. Mutagen: EMS Outcrossed: x5 Made by: Yiwen Jia Received: 04/12/96 LSU Health ----------------------------------------------------------------------------- Strain: EA90 Species: Caenorhabditis elegans Genotype: pag-1(ls2) dpy-17(e164) III. Description: Dpy. pag-1(ls2) is recessive and has not visible phenotype by itself. It increases the expression of certain lacZ fusion genes such as lacZmec-7, unc-86lacZ and unc-4unc-76lacZ. Mutagen: EMS Outcrossed: x5 Made by: Guofeng Xie Received: 04/12/96 LSU Health ----------------------------------------------------------------------------- Strain: EAG16 Species: C. elegans Genotype: eagIs6[*fxIs10] II. Description: eagIs6 [spn-4p::jGCaMP7s::pie-1 3'UTR + HygR [*fxIs10] ] II. CaFE reporter (calcium inducible fluorescence in germline). Calcium-inducible fluorescent jGCaMP7s protein codon-optimized for elegans and expressed in germline enables visualization of calcium wave upon fertilization. Reference: Toperzer KM, et al. Biol Open. 2023 Sep 15;12(9):bio059832. PMID: 37602653. Mutagen: Outcrossed: x0 Made by: Karen Kim Guisbert Received: 07/12/24 University of Nebraska, Omaha, NE ----------------------------------------------------------------------------- Strain: EAG25 Species: C. elegans Genotype: eagIs6[*fxIs10] ujIs113 II. Description: eagIs6 [spn-4p::jGCaMP7s::pie-1 3'UTR + HygR [*fxIs10] ] II. ujIs113 [pie-1p::mCherry::H2B::pie-1 3'UTR + nhr-2p::his-24::mCherry::let-858 3'UTR + unc-119(+)]. CaFE reporter (calcium inducible fluorescence in germline). Calcium-inducible fluorescent jGCaMP7s protein codon-optimized for elegans and expressed in germline enables visualization of calcium wave upon fertilization. H2::mCherry marks germline nuclei. Reference: Toperzer KM, et al. Biol Open. 2023 Sep 15;12(9):bio059832. PMID: 37602653. Mutagen: Outcrossed: x0 Made by: Karen Kim Guisbert Received: 07/12/24 University of Nebraska, Omaha, NE ----------------------------------------------------------------------------- Strain: EAG28 Species: C. elegans Genotype: eagIs6[*fxIs10] II; ltIs44 IV. Description: eagIs6 [spn-4p::jGCaMP7s::pie-1 3'UTR + HygR [*fxIs10] ] II. ltIs44 [pie-1p::mCherry::PH(PLC1delta1) + unc-119(+)] IV. CaFE reporter (calcium inducible fluorescence in germline). Calcium-inducible fluorescent jGCaMP7s protein codon-optimized for elegans and expressed in germline enables visualization of calcium wave upon fertilization. mCherry::PH marks cell membranes. Reference: Toperzer KM, et al. Biol Open. 2023 Sep 15;12(9):bio059832. PMID: 37602653. Mutagen: Outcrossed: x0 Made by: Karen Kim Guisbert Received: 07/12/24 University of Nebraska, Omaha, NE ----------------------------------------------------------------------------- Strain: EAK102 Species: C. elegans Genotype: eeeIs1. Description: eeeIs1 [unc-54p::Htt513(Q15)::YFP::unc-45 3'UTR]. YFP expression in body wall muscle cells. YFP is fused to a fragment of mutant human Huntingtin protein. Reference: Lee AL. et al. PLoS One. 2017 Mar 10;12(3):e0173644. [NOTE: The transgene in this strain was previously described as using the unc-45 promoter, but it is actually the unc-54 promoter.] Mutagen: Outcrossed: x5 Made by: Elise Kikis Received: 10/16/17 University of the South, Sewanee, TN ----------------------------------------------------------------------------- Strain: EAK103 Species: C. elegans Genotype: eeeIs2. Description: eeeIs2 [unc-54p::Htt513(Q128)::YFP::unc-45 3'UTR]. Motility defect. YFP expression in body wall muscle cells. YFP is fused to a fragment of mutant human Huntingtin protein. Reference: Lee AL. et al. PLoS One. 2017 Mar 10;12(3):e0173644. [NOTE: The transgene in this strain was previously described as using the unc-45 promoter, but it is actually the unc-54 promoter.] Mutagen: Outcrossed: x5 Made by: Elise Kikis Received: 10/16/17 University of the South, Sewanee, TN ----------------------------------------------------------------------------- Strain: EB4491 Species: Caenorhabditis elegans Genotype: dzDf1 lem-4(ve691[myo-2p::GFP])/tmC25 [unc-5(tm9708)] IV. Description: dzDf1 [IV:506328 - 698511 deleted]. Pick wild-type GFP+ to maintain. Heterozygotes are wildtype GFP+ and segregate into dead eggs (dzDf1 homozygotes), wild-type GFP+ (Heterozygotes), and Unc (tmC25 homozygotes). Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Leo T. H. Tang Received: 08/17/23 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EB4499 Species: Caenorhabditis elegans Genotype: dzDf2/szT1 [lon-2(e678) umnIs17] I; +/szT1 X. Description: dzDf2 [I:2037935 - 2261431 deleted]. Pick wild-type GFP+ to maintain. Heterozygotes are wildtype GFP+, segregate into wild-type GFP+, dead eggs, and GFP+ Lon males. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Leo T. H. Tang Received: 08/17/23 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EB4500 Species: Caenorhabditis elegans Genotype: dzDf3/szT1 [lon-2(e678) umnIs17] I; +/szT1 X. Description: dzDf3 [I:1999831 - 2100118 deleted]. Heterozygotes are wild-type GFP+. Segregate wild-type GFP+, dead eggs, and GFP+ Lon males. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Leo T. H. Tang Received: 11/15/23 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EC100 Species: Caenorhabditis elegans Genotype: eeEx100. Description: eeEx100 [his-24::GFP + rol-6(su1006)]. Rollers. Nuclear GFP fluorescence detected beginning with the eight-cell stage of the embryo in all somatic nuclei without the P-cell. In adults the GFP signal in somatic cells and in few hermaphrodites in undifferentiated germ nuclei and in oocytes and sperm. About 20% transmission. Mutagen: Outcrossed: x Made by: Monika Jedrusik Received: 10/02/02 University of Gottingen, Germany ----------------------------------------------------------------------------- Strain: EC106 Species: Caenorhabditis elegans Genotype: eeEx106. Description: eeEx106 [hil-1::GFP + rol-6(su1006)]. GFP expression in body wall muscles, in the vulva sex muscles, in the marginal cells of the pharynx, in a limited number of head neurons, in the cytoplasm of excretory cells. The expression starts in the about 100-cell embryo in a few cells in the periphery in the nucleoplasm and in the nucleoli. Complex extrachromosomal arrary....pick Rollers. About 20% Rollers. Mutagen: Outcrossed: x Made by: Monika Jedrusik Received: 10/02/02 University of Gottingen, Germany ----------------------------------------------------------------------------- Strain: ED3005 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated from a compost bin from a West Mains allotment public vegetable garden near Edinburgh Scotland on Dec. 19, 2005. Haplotype (according to Cutter 2006 and Dolgin et al 2008): J. Reference: Andersen EC, et al. Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: Asher Cutter Received: 09/04/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: ED3010 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated from a compost bin from a Midmar Allotment public vegetable garden (field 1 plot 39) near Edinburgh Scotland on Nov. 26, 2005. Haplotype (according to Cutter 2006 and Dolgin et al 2008): A. Mutagen: Outcrossed: x Made by: A. Cutter/E. Dolgin Received: 09/15/08 University of Edinburgh, Edinburgh, Scotland ----------------------------------------------------------------------------- Strain: ED3011 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated from a compost bin from a Midmar Allotment public vegetable garden (field 1 plot 39) near Edinburgh Scotland on Nov. 26, 2005. Haplotype (according to Cutter 2006 and Dolgin et al 2008): J. Mutagen: Outcrossed: x Made by: A. Cutter/E. Dolgin Received: 09/15/08 University of Edinburgh, Edinburgh, Scotland ----------------------------------------------------------------------------- Strain: ED3012 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: A. Cutter Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: ED3014 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated from a compost bin from a Midmar Allotment public vegetable garden (field 1 plot 39) near Edinburgh Scotland on Dec. 3, 2005. Haplotype (according to Cutter 2006 and Dolgin et al 2008): A. Mutagen: Outcrossed: x Made by: A. Cutter/E. Dolgin Received: 09/15/08 University of Edinburgh, Edinburgh, Scotland ----------------------------------------------------------------------------- Strain: ED3017 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated from a compost bin from a Midmar Allotment public vegetable garden (field 1 plot 39) near Edinburgh Scotland on Dec. 19, 2005. Haplotype (according to Cutter 2006 and Dolgin et al 2008): N. Mutagen: Outcrossed: x Made by: A. Cutter/E. Dolgin Received: 12/10/07 University of Edinburgh, Edinburgh, Scotland ----------------------------------------------------------------------------- Strain: ED3021 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated from a compost bin from a Midmar Allotment public vegetable garden (field 1 plot 39) near Edinburgh Scotland on Dec. 3, 2005. Haplotype (according to Cutter 2006 and Dolgin et al 2008): J. Mutagen: Outcrossed: x Made by: A. Cutter/E. Dolgin Received: 09/15/08 University of Edinburgh, Edinburgh, Scotland ----------------------------------------------------------------------------- Strain: ED3024 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated from a compost bin from a Midmar Allotment public vegetable garden (field 2 plot 43) near Edinburgh Scotland on Dec. 19, 2005. Haplotype (according to Cutter 2006 and Dolgin et al 2008): A. Mutagen: Outcrossed: x Made by: A. Cutter/E. Dolgin Received: 12/10/07 University of Edinburgh, Edinburgh, Scotland ----------------------------------------------------------------------------- Strain: ED3032 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: Isolated as a single hermaphrodite from a flower bed soil sample in the botanic gardens in Chungcheng, Taipei, Taiwan, September 29, 2005. Mutagen: Outcrossed: x Made by: Asher Cutter Received: 06/26/06 University of Edinburgh, Edinburgh, Scotland ----------------------------------------------------------------------------- Strain: ED3033 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: Isolated as a single hermaphrodite from rotting wood in the garden of a private residence in Tien Mu, Taipai, Taiwan, Oct. 2, 2005. Mutagen: Outcrossed: x Made by: Asher Cutter Received: 06/26/06 University of Edinburgh, Edinburgh, Scotland ----------------------------------------------------------------------------- Strain: ED3034 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: Isolated as a single hermaphrodite from rotting wood in the garden of a private residence in Tien Mu, Taipai, Taiwan, Oct. 2, 2005. Mutagen: Outcrossed: x Made by: Asher Cutter Received: 06/26/06 University of Edinburgh, Edinburgh, Scotland ----------------------------------------------------------------------------- Strain: ED3035 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: Isolated as a single hermaphrodite from rotting wood in the garden of a private residence in Tien Mu, Taipai, Taiwan, Oct. 2, 2005. Mutagen: Outcrossed: x Made by: Asher Cutter Received: 06/26/06 University of Edinburgh, Edinburgh, Scotland ----------------------------------------------------------------------------- Strain: ED3036 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: Isolated as a single hermaphrodite from rotting wood in the garden of a private residence in Tien Mu, Taipai, Taiwan, Oct. 2, 2005. Mutagen: Outcrossed: x Made by: Asher Cutter Received: 06/26/06 University of Edinburgh, Edinburgh, Scotland ----------------------------------------------------------------------------- Strain: ED3037 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: Isolated as a single hermaphrodite from rotting wood in the garden of a private residence in Tien Mu, Taipai, Taiwan, Oct. 2, 2005. Mutagen: Outcrossed: x Made by: Asher Cutter Received: 06/26/06 University of Edinburgh, Edinburgh, Scotland ----------------------------------------------------------------------------- Strain: ED3040 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated from compost from Jenny Pettifor's garden in the Paview neighborhood in Johannesburg, South Africa, on May 5, 2006. Haplotype (according to Cutter 2006 and Dolgin et al 2008): alpha. Mutagen: Outcrossed: x Made by: E. Dolgin Received: 11/26/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: ED3041 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated near Ceres, South Africa, on April 2, 2006. Lat: -33.3667; Lon: 19.31667. Haplotype (according to Cutter 2006 and Dolgin et al 2008): beta. Mutagen: Outcrossed: x Made by: A Cutter & E Dolgin Received: 01/17/13 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: ED3042 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated from compost at a plant nursery at the Ceres Fruit Farms in the Western Cape province near Ceres, South Africa, on May 5, 2006. Haplotype (according to Cutter 2006 and Dolgin et al 2008): beta. Mutagen: Outcrossed: x Made by: E. Dolgin Received: 12/10/07 University of Edinburgh, Edinburgh, Scotland ----------------------------------------------------------------------------- Strain: ED3043 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated near Ceres, South Africa, on April 2, 2006. Lat: -33.3667; Lon: 19.31667. Haplotype (according to Cutter 2006 and Dolgin et al 2008): beta. Mutagen: Outcrossed: x Made by: A Cutter & E Dolgin Received: 02/15/13 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: ED3044 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated near Ceres, South Africa, on April 2, 2006. Lat: -33.3667; Lon: 19.31667. Haplotype (according to Cutter 2006 and Dolgin et al 2008): beta. Mutagen: Outcrossed: x Made by: A Cutter & E Dolgin Received: 02/15/13 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: ED3045 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated near Ceres, South Africa, on April 2, 2006. Lat: -33.3667; Lon: 19.31667. Haplotype (according to Cutter 2006 and Dolgin et al 2008): beta. Mutagen: Outcrossed: x Made by: A Cutter & E Dolgin Received: 01/17/13 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: ED3046 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated from compost at a plant nursery at the Ceres Fruit Farms in the Western Cape province near Ceres, South Africa, on May 5, 2006. Haplotype (according to Cutter 2006 and Dolgin et al 2008): gamma. Mutagen: Outcrossed: x Made by: E. Dolgin Received: 12/10/07 University of Edinburgh, Edinburgh, Scotland ----------------------------------------------------------------------------- Strain: ED3048 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: E. Dolgin Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: ED3049 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: E. Dolgin Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: ED3050 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated near Ceres, South Africa, on April 2, 2006. Lat: -33.3667; Lon: 19.31667. Haplotype (according to Cutter 2006 and Dolgin et al 2008): beta. Mutagen: Outcrossed: x Made by: A Cutter & E Dolgin Received: 01/17/13 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: ED3051 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated near Ceres, South Africa, on April 2, 2006. Lat: -33.3667; Lon: 19.31667. Haplotype (according to Cutter 2006 and Dolgin et al 2008): beta. Mutagen: Outcrossed: x Made by: A Cutter & E Dolgin Received: 02/15/13 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: ED3052 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated from compost at a plant nursery at the Ceres Fruit Farms in the Western Cape province near Ceres, South Africa, on May 5, 2006. Haplotype (according to Cutter 2006 and Dolgin et al 2008): delta. Mutagen: Outcrossed: x Made by: E. Dolgin Received: 12/10/07 University of Edinburgh, Edinburgh, Scotland ----------------------------------------------------------------------------- Strain: ED3053 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated near Limuru, Kenya, on May 17, 2006. Lat: -1.08333; Lon: 36.65. Haplotype (according to Cutter 2006 and Dolgin et al 2008): epsilon. Mutagen: Outcrossed: x Made by: A Cutter & E Dolgin Received: 01/17/13 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: ED3054 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate Description: Caenorhabditis elegans wild isolate. Reference: Dolgin ES, et al. Heredity (Edinb). 2008 Mar;100(3):304-15. Mutagen: Outcrossed: x Made by: A Cutter & E Dolgin Received: 11/11/16 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: ED3071 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated near Limuru, Kenya, on May 17, 2006. Lat: -1.08333; Lon: 36.65. Haplotype (according to Cutter 2006 and Dolgin et al 2008): epsilon. Mutagen: Outcrossed: x Made by: A Cutter & E Dolgin Received: 01/17/13 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: ED3072 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated from compost from the Olive Mushroom Farms near the town of Limuru, Kenya on May 17, 2006. Haplotype (according to Cutter 2006 and Dolgin et al 2008): epsilon. Mutagen: Outcrossed: x Made by: E. Dolgin Received: 12/10/07 University of Edinburgh, Edinburgh, Scotland ----------------------------------------------------------------------------- Strain: ED3073 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: E. Dolgin Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: ED3075 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated near Limuru, Kenya, on May 17, 2006. Lat: -1.08333; Lon: 36.65. Haplotype (according to Cutter 2006 and Dolgin et al 2008): epsilon. Mutagen: Outcrossed: x Made by: A Cutter & E Dolgin Received: 01/17/13 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: ED3076 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated near Limuru, Kenya, on May 17, 2006. Lat: -1.08333; Lon: 36.65. Haplotype (according to Cutter 2006 and Dolgin et al 2008): epsilon. Mutagen: Outcrossed: x Made by: A Cutter & E Dolgin Received: 02/15/13 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: ED3077 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated from leaf litter from the Uhuru Gardens public park in the south part of Nairobi, Kenya on May 17, 2006. Haplotype (according to Cutter 2006 and Dolgin et al 2008): beta. Mutagen: Outcrossed: x Made by: E. Dolgin Received: 12/10/07 University of Edinburgh, Edinburgh, Scotland ----------------------------------------------------------------------------- Strain: ED3083 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: Caenorhabditis briggsae wild isolate. Isolated from compost from Jenny Pettifor's garden in the Paview neighborhood in Johannesburg, South Africa, on May 5, 2006. Landscape: Urban_garden. Isolated from a compost sample from a private garden in Johannesburg, South Africa, March-April 06 by E. Dolgin. Same compost sample as ED3078-3089. WBPaper00035666. GPS: -26.200001 28.000000, Johannesburg, South Africa. Substrate: compost_heap. Sampled_by: Elie Dolgin WBPerson12345. 2006. Mutagen: Outcrossed: x Made by: E. Dolgin Received: 12/10/07 University of Edinburgh, Edinburgh, Scotland ----------------------------------------------------------------------------- Strain: ED3092 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: Caenorhabditis briggsae wild isolate. Isolated from leaf litter from the Uhuru Gardens public park in the south part of Nairobi, Kenya on May 17, 2006. Mutagen: Outcrossed: x Made by: E. Dolgin Received: 12/10/07 University of Edinburgh, Edinburgh, Scotland ----------------------------------------------------------------------------- Strain: ED3101 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: Caenorhabditis briggsae wild isolate. Isolated from leaf litter from the Uhuru Gardens public park in the south part of Nairobi, Kenya on May 17, 2006. Mutagen: Outcrossed: x Made by: E. Dolgin Received: 12/10/07 University of Edinburgh, Edinburgh, Scotland ----------------------------------------------------------------------------- Strain: EE31 Species: Caenorhabditis elegans Genotype: mup-2(e2346) unc-6(e78)/lon-2(e678) X. Description: Heterozygotes are WT and segregate WT, Lon and Uncs. If raised at 25C, the Uncs are Mup -> 3-fold/L1 arrest. If raised at 15C the Uncs are fertile (reduced brood). Mutagen: EMS Outcrossed: x2 Made by: Received: 06/19/97 University of Pennsylvania, Philadelphia ----------------------------------------------------------------------------- Strain: EE66 Species: Caenorhabditis elegans Genotype: mup-2(e2346) unc-6(e78) X. Description: Temperature sensitive. Maintain at 15C. At 25C the animals will arrest at 3-fold/L1 stage. Unc. Mutagen: EMS Outcrossed: x2 Made by: Thierry Bogaert Received: 06/19/97 University of Pennsylvania, Philadelphia ----------------------------------------------------------------------------- Strain: EE67 Species: Caenorhabditis elegans Genotype: him-8(e1489) IV; mup-2(e2346) X. Description: Temperature sensitive. At 15C the animals are essentially WT, but with a reduced brood; males mate well. Embryos raised at 25C will result in 3-fold/L1 arrest (Mup). Larval shift to 25C will result in sterile hermaphrodites but males that are fertile. Mutagen: EMS Outcrossed: x2 Made by: Thierry Bogeart Received: 06/19/97 University of Pennsylvania, Philadelphia ----------------------------------------------------------------------------- Strain: EE86 Species: Caenorhabditis elegans Genotype: mup-4(mg36) III; upIs1. Description: upIs1 [mup-4::GFP + rol-6(su1006)]. Rollers. [NOTE: 11/2006: Pamela Hoppe determined that the strain is homozygous for mup-4.] Mutagen: Outcrossed: x2 Made by: Elbl/Bucher Received: 06/12/02 University of Pennsylvania, Philadelphia ----------------------------------------------------------------------------- Strain: EEG107 Species: C.elegans Genotype: tph-1(mg280) II; mudIs1. Description: mudIs1 [tph-1p::ChR2::GFP + myo-3p::mCherry]. When tph-1 mutants carrying mudIs1 are exposed to blue light, the worms continue to move rather than stopping like wild-type animals. Reference: Pokala N & Glater EE. 2018. Journal of Undergraduate Neuroscience Education. 162(2): A152-A158. Mutagen: Outcrossed: x0 Made by: Elizabeth Glater Received: 08/09/18 Pomona College, Claremont, CA ----------------------------------------------------------------------------- Strain: EEG108 Species: C. elegans Genotype: mod-5(n822) I; mudIs1. Description: mudIs1 [tph-1p::ChR2::GFP + myo-3p::mCherry]. Worms stop moving when exposed to blue light. Reference: Pokala N & Glater EE. 2018. Journal of Undergraduate Neuroscience Education. 162(2): A152-A158. Mutagen: Outcrossed: x0 Made by: Elizabeth Glater Received: 08/09/18 Pomona College, Claremont, CA ----------------------------------------------------------------------------- Strain: EEG98 Species: C. elegans Genotype: mudIs1. Description: mudIs1 [tph-1p::ChR2::GFP + myo-3p::mCherry]. Worms stop moving when exposed to blue light. Reference: Pokala N & Glater EE. 2018. Journal of Undergraduate Neuroscience Education. 162(2): A152-A158. Mutagen: none Outcrossed: x0 Made by: Elizabeth Glater Received: 08/09/18 Pomona College, Claremont, CA ----------------------------------------------------------------------------- Strain: EG1000 Species: Caenorhabditis elegans Genotype: dpy-5(e61) I; rol-6(e187) II; lon-1(e1820) III. Description: Dpy suppresses Rol and Lon. Strain appears to be only Dpy. Useful for mapping, especially Unc mutations. Separately, dpy-5 causes extreme dumpiness, rol-6 causes worms to roll over and lie in a "C" shape, and lon-1 worms are about 125% WT length. Mutagen: Outcrossed: x Made by: Erik Jorgensen Received: 03/25/03 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG1020 Species: Caenorhabditis elegans Genotype: bli-6(sc16) IV; dpy-11(e224) V; lon-2(e678) X. Description: Dpy suppresses Bli and Lon. Strain appears to be only slightly Dpy. Useful for mapping, especially Unc mutations. Separately, dpy-11 causes dumpiness, bli-6 adult worms develop blisters on their bodies, and lon-2 worms are about 150% WT length. Mutagen: Outcrossed: x Made by: Erik Jorgensen Received: 03/25/03 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG1285 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) oxIs12 X. Description: oxIs12 [unc-47p::GFP + lin-15(+)]. Integration maps at +2.0 on X. Expression of GFP in all GABAergic neurons. Mutagen: Integrated by X-rays Outcrossed: x Made by: Kim Schuske Received: 06/09/03 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG144 Species: Caenorhabditis elegans Genotype: inx-16(ox144) I. Description: Pax, Dec-slow (34" defecation cycle). Maintain uder normal conditions. Reference: Peters MA, et al. Curr Biol. 2007 Sep 18;17(18):1601-8. Mutagen: Outcrossed: x3 Made by: Paola DalSanto Received: 11/19/10 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG1470 Species: Caenorhabditis elegans Genotype: oxEx229. Description: oxEx229 [Mos1 Substrate + myo-2::GFP]. Should be grown at 25C. Mutagen: Outcrossed: x1 Made by: Dan Williams Received: 08/28/01 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG1642 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; oxEx166. Description: oxEx166 [HSP::MosTRANSPOSASE + CC::GFP + lin-15(+)]. Should be grown at 25C. Maintain by picking non-Muv. Animals carrying the array should be GFP+. Mutagen: Outcrossed: x Made by: J-L Bessereau Received: 08/28/01 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG1653 Species: Caenorhabditis elegans Genotype: oxIs22 II. Description: oxIs22 [unc-49p::unc-49::GFP + lin-15(+)]. Psoralen integration of oxEx129 [unc-49Bp(long)::GFP + lin-15(+)]. Dorsal and ventral. Mutagen: Integrated using Psoralen. Outcrossed: x4 Made by: Bamber/Bessereau Received: 06/09/03 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG199 Species: Caenorhabditis elegans Genotype: nas-37(ox199) X. Description: At each molt the cuticle fails to open sufficiently at the anterior end and the partially shed cuticle is dragged behind the animal. Nucleotide change: substitution [c/t]. Flanking sequences: TTGTGGAGGATGCGGAACTAAAACC[c/t]GAGTTAGAGCATGCTACGGTGGAAA. Mutagen: ENU Outcrossed: x2 Made by: Wayne Davis Received: 11/30/04 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG2288 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; oxEx110. Description: oxEx110 [unc-49c::GFP + lin-15(+)]. Maintain by picking non-Muv. Mutagen: Outcrossed: x Made by: Bruce Bamber Received: 12/08/03 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG2537 Species: Caenorhabditis elegans Genotype: oxEx344. Description: oxEx344 [MosPolyA substrate + myo-2::GFP]. Should be grown at 25C. Mutagen: Outcrossed: x Made by: Dan Williams Received: 08/28/01 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG2710 Species: Caenorhabditis elegans Genotype: unc-57(ok310) I. Description: T04D1.3 Homozygous. Outer left primer sequence: GCGAATCAATACCTTTCGGA. Inner left primer sequence: GCTACTCGAGCAAAAATGGC. Outer right primer sequence: CCTGGTGGAGGTCCTTGATA. Inner right primer sequence: TCAAGGGTATCGCTTTTTCG. Deletion length: 1959 bp. Deletion breakpoints: AAGCTGTCAAAGTTTAATTTTTTTTTAATCTGCTGAAATTTTTTTCCACTTCCCCTTTT AGATATAATCACAAAAAAATTCTTTT[left break]....deletion....[right break]GAATTTTTTAAATCAATTTTCTAAATCGAAACTATTCGTTTTTCAATTTTTAT TTTAAAAAATCGAAAAAGCGATACCCTTGATTA. This strain was provided by the C. elegans Gene Knockout Project at OMRF, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. http://www.mutantfactory.ouhsc.edu Mutagen: UV/TMP Outcrossed: x2 Made by: E Jorgensen Received: 09/29/03 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG276 Species: Caenorhabditis elegans Genotype: exp-1(ox276) II. Description: Maintain under normal conditions. Reference: Beg AA & Jorgensen EM, Nat Neurosci. 2003 Nov;6(11):1145-52. Mutagen: EMS Outcrossed: x4 Made by: Asim Beg Received: 02/17/11 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG2762 Species: Caenorhabditis elegans Genotype: oxEx166. Description: oxEx166 [HSP::MosTransposase + coelomocyte::GFP + lin-15(+)]. Should be grown at 25C. Mutagen: Outcrossed: x1 Made by: Dan Williams Received: 08/28/01 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG3027 Species: Caenorhabditis elegans Genotype: unc-26(s1710) IV. Description: Severe kinker. Small and scrawny with flaccid little movement. Slow pharyngeal pumping. Mutagen: Outcrossed: x>2 Made by: Kim Schuske Received: 08/29/08 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG3283 Species: Caenorhabditis elegans Genotype: nas-37(tm410) X. Description: At each molt the cuticle fails to open sufficiently at the anterior end and the partially shed cuticle is dragged behind the animal. Deletion. Flanking sequences: ttcttgtccagtagggtctagtcgtggttg tgaacttgcctgtcgatgtcttctggctga. Mutagen: UV/TMP Outcrossed: x1 Made by: Wayne Davis Received: 11/30/04 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG334 Species: Caenorhabditis elegans Genotype: cccp-1(ox334) III. Description: Unmotivated locomotion, egg-laying defect, weak Hid phenotype. Reference: Ailion M, et al. Neuron. 2014 Apr 2;82(1):167-80. Mutagen: ENU Outcrossed: x2 Made by: Michael Ailion Received: 05/10/16 University of Washington ----------------------------------------------------------------------------- Strain: EG4 Species: Caenorhabditis elegans Genotype: pbo-5(ox4) V. Description: Abnormal posterior body muscle contractions during defecation. Derived by single outcrossing of MT1733; allelic to n2303. Mutagen: EMS Outcrossed: x1 Made by: Paola DaSanta Received: 10/15/12 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG4181 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: Isolated by Michael Ailion from rotting apricot from home of Wayne Davis and Danielle Endres in Salt Lake City, Utah, 8/4/2006, under tree on north side of house. Coordinates: 40° 42' 26.16" N, 111° 52' 3.27" W. Animals move very rapidly. Strain started from a single L4 hermaphrodite and grown three generations before freezing. 18S rDNA sequence differs from C. briggsae NCBI entry (PB102), but is identical to the 18S sequence of AF16, HK104, PB800 and VT847. Cross-fertile with AF16. Mutagen: Outcrossed: x Made by: Michael Ailion Received: 10/10/06 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG4207 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: Mixed "strain" started from many different worms that emerged from the same apricot that gave rise to strain EG4181. Some worms emerged as dauers, but there were also L4s and adults. Consists of a mix of the worms that came from the apricot to try to maintain as much diversity as possible in the population and thus is not a true strain in the traditional sense. It will be sent as contaminated worms since it cannot be cleaned up without going through a bottleneck. Mutagen: Outcrossed: x Made by: Michael Ailion Received: 10/10/06 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG4322 Species: Caenorhabditis elegans Genotype: ttTi5605 II; unc-119(ed9) III. Description: Unc. Not caused by ttTi5605. Mos1 allele generated by NemaGENETAG consortium (Laurent Segalat). [NOTE: 11/15/11 - This strain contains unc-119(ed9), not unc-119(ed3) as previously reported. (C. Frokjaer-Jensen)] [NOTE: The Dernburg lab has noticed an increased number of rad-51 foci in EG4322 compared to N2. Please use the outcrossed version of this strain (EG6699) instead, which does not have this problem. (C. Frokjaer-Jensen)] Mutagen: Outcrossed: x1 Made by: Mos1 mutagenesis Received: 09/15/08 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG4347 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: M. Ailion Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: EG4348 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Utah natural isolate carrying peel-1(qq99) I. EG4348 was collected by M. Ailion from Salt Lake City, UT. qq99 designates the naturally occurring nonsense mutation in peel-1. Reference: Seidel HS et al. PLoS Biol. 2011 Jul;9(7):e1001115. Mutagen: Outcrossed: x Made by: M. Ailion Received: 05/29/12 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: EG4349 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: M. Ailion Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: EG4443 Species: Caenorhabditis elegans Genotype: oxIs253 II; unc-119(ed3) III. Description: oxIs253 [unc-122p::GFP + unc-119(+)]. Wild type. Very dim GFP expression in the coelomycytes. Only visible on compound microscope. Plasmid pCFJ68 inserted by MosSCI into ttTi5605 site. Mutagen: Mos1 transposase Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 12/16/08 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG4601 Species: Caenorhabditis elegans Genotype: oxIs279 II; unc-119(ed3) III. Description: oxIs279 [pie-1p::GFP::H2B + unc-119(+)]. Wild type. Persistent GFP expression in germline. Visible on dissection microscope. Plasmid pCFJ127 inserted by MosSCI into ttTi5605 site. Mutagen: Mos1 transposase Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 12/16/08 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG4724 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: M. Ailion Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: EG4725 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: M. Ailion Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: EG4788 Species: Caenorhabditis portoensis Genotype: Caenorhabditis sp. 6 wild isolate. Description: Male-female strain. Maintain by mating. Caenorhabditis sp. 6 wild isolate. Isolated by Michael Ailion and Ana-Joao Rodrigues from a rotting apple collected from the home of Anabela Fernandes in Amares, Portugal (address: Dr. Adolofo Vilela, no. 29, 4720-019 Amares, Portugal). Coordinates: 41 degrees, 37', 43.577 " N; 8 degrees, 20', 51.421 " W. Apple collected March 28, 2007. The apple produced a number of growing animals in different stages, including adults of both sexes. Male/Female Caenorhabditis species. 18S RNA sequence places it closest to PS1010 C. sp. 3. Mutagen: Outcrossed: x Made by: Michael Ailion Received: 09/24/07 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG4813 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; oxEx1088. Description: oxEx1088 [unc-17p::halorhodopsin::GFP + Litmus + lin-15(+)]. Maintain by picking wild-type. Mutagen: Outcrossed: x0 Made by: Gunther Hollopeter Received: 08/24/09 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG4814 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; oxEx1089. Description: oxEx1089 [unc-47p::halorhodopsin::GFP + Litmus + lin-15(+)]. Maintain by picking wild-type. Mutagen: Outcrossed: x0 Made by: Gunther Hollopeter Received: 08/24/09 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG4883 Species: Caenorhabditis elegans Genotype: oxIs318 II; unc-119(ed3) III. Description: oxIs318 [spe-11p::mCherry::histone + unc-119(+)]. Wild type. Dim mCherry expression in hermaphrodite sperm. Barely visible on bright dissection microscope; visible on compound microscope. Plasmid pCFJ167 inserted by MosSCI into ttTi5605 site. Mutagen: Mos1 transposase Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 12/16/08 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG4887 Species: Caenorhabditis elegans Genotype: oxIs322 II; unc-119(ed3) III. Description: oxIs322 [myo-2p::mCherry::H2B + myo-3p::mCherry::H2B + Cbr-unc-119(+)]. Wild type worms with mCherry fluorescence in pharyngeal and body wall muscle. Visible on dissection microscope at high magnification. Complex transgene insertion in place of Mos1 allele ttTi5605. Useful for following "invisible" insertions at ttTi5605 site by Mos1 Single Copy gene Insertion (MosSCI). Please note: The insertion was a complex event pulling in more than one transgene and parts of the array. Therefore, the exact molecular structure of the insert is not known. Therefore the strain should NOT be used as a control for insert copy number or other detailed molecular controls of MosSCI insertions. Succesfully used as a balancer for the ttTi5605 locus. Mutagen: Outcrossed: x0 Made by: Mos1 mutagenesis Received: 09/15/08 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG4946 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Reference: Andersen EC, et al. Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: G. Hollopeter Received: 09/04/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: EG5003 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; cxTi10882 IV. Description: Unc. Not caused by cxTi10882. EG5003 contains background mutations (partial deletion of pgp-6 and pgp-7 and a deletion close to cTel3x.1). EG6250 is an outcrossed version of this strain. Mos1 allele generated by NemaGENETAG consortium (Laurent Segalat). Mutagen: Outcrossed: x1 Made by: Mos1 mutagenesis Received: 09/15/08 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG5025 Species: Caenorhabditis elegans Genotype: oxIs351 X. Description: oxIs351 contains [unc-47p::channelrhodopsin::mCherry + lin-15(+) + Litmus]. Superficially wild-type. Mutagen: X-ray Outcrossed: x2 Made by: Gunther Hollopeter Received: 08/24/09 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG5027 Species: Caenorhabditis elegans Genotype: oxIs353 V. Description: oxIs353 contains [myo-3p::channelrhodopsin::mCherry + lin-15(+) + Litmus]. Superficially wild-type. Mutagen: X-ray Outcrossed: x2 Made by: Gunther Hollopeter Received: 08/24/09 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG5071 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxIs363 IV. Description: oxIs363 [unc-122p::GFP + unc-119(+)]. Wild type. Very dim GFP expression in the coelomycytes. Only visible on compound microscope. Plasmid pBN04 inserted by MosSCI into cxTi10882 site. Mutagen: Mos1 transposase Outcrossed: x0 Made by: Eric Bend Received: 12/16/08 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG5096 Species: Caenorhabditis elegans Genotype: oxIs364 X. Description: oxIs364 contains [unc-17p::channelrhodopsin::mCherry + lin-15(+) + Litmus]. Superficially wild-type. Mutagen: X-ray Outcrossed: x2 Made by: Gunther Hollopeter Received: 08/24/09 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG5268 Species: Caenorhabditis nigoni Genotype: Caenorhabditis nigoni wild isolate. Description: Caenorhabditis sp. 9 Male-female strain. Isolated from a sample collected by Joel Ehrenkranz on May 20, 2008, around 11 AM in Shinkolobwe, Katanga Province, Democratic Republic of the Congo (~11°S, 26°E). The sample was collected from worked soil which contained organic material around the foundation of a wattle hut. This time of year was the dry season in Katanga Province, so daytime temperatures were in the high 70s and nighttime temperatures in the low 50s. The specimen was placed in a ziplock bag with a slice of apple for transport to Susan Mango's lab at the Huntsman Cancer Institute, Salt Lake City, Utah. From the date of collection until the specimen reached the lab was approximately 2 weeks. Mutagen: Outcrossed: x Made by: T Brock & M Ailion Received: 10/30/12 University of Washington ----------------------------------------------------------------------------- Strain: EG5389 Species: Caenorhabditis elegans Genotype: oxIs494 II; unc-119(ed3) III. Description: oxIs494 [peel-1p::GFP::peel-1 3'UTR + Cbr-unc-119(+)] II. GFP is expressed in the spermatogenic germline. During spermatogenesis, GFP remains in the residual body and is not packaged into sperm. Reference: Seidel HS, et al. PLoS Biol. 2011 Jul;9(7):e1001115. Mutagen: Outcrossed: x0 Made by: Ailion & Seidel Received: 07/26/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: EG5505 Species: Caenorhabditis elegans Genotype: rund-1(tm3622) X. Description: Unmotivated locomotion, egg-laying defect, weak Hid phenotype. 4X outcrossed for autosomes, 2X outcrossed for the X chromosome. Reference: Ailion M, et al. Neuron. 2014 Apr 2;82(1):167-80. Mutagen: Outcrossed: x4 Made by: Michael Ailion Received: 05/10/16 University of Washington ----------------------------------------------------------------------------- Strain: EG5568 Species: Caenorhabditis elegans Genotype: dpy-13(ox495::Cbr-unc-119(+) + myo-2p::mCherry + unc-122p::GFP) IV. Description: dpy-13(ox495::Cbr-unc-119(+) + myo-2p::mCherry + unc-122p::GFP) IV. Dpy, mCherry pharyngeal muscle, dim GFP+ in coelomycytes. Insertion/deletion into cxTi10882 MosSCI site on Chr. IV. Can be used as balancer. Mutagen: Mos1 transposon Outcrossed: x2 Made by: C. Frokjaer-Jensen Received: 03/23/12 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG5716 Species: Caenorhabditis imperialis Genotype: Caenorhabditis imperialis wild isolate. Description: Caenorhabditis sp. 14 Male-female strain. Isolated from a rotten horse chestnut (Tahitian chestnut?) collected by David Ailion on the island of Moorea (17.53 S, 149.83 W) on July 24, 2009. Mutagen: Outcrossed: x Made by: Michael Ailion Received: 10/30/12 University of Washington ----------------------------------------------------------------------------- Strain: EG5767 Species: Caenorhabditis elegans Genotype: qqIr7 I; oxSi78 II; unc-119(ed3) III. Description: qqIr7 [peel-1(qq99)] I. oxSi78 [peel-1p::peel-1 (introns included)::GFP::peel-1 3'UTR + Cbr-unc-119(+)] II. Genetic background is a mixture of N2 and wild isolate EG4348. The oxSi78 insertion produces a PEEL-1::GFP translational fusion. PEEL-1::GFP is expressed in the spermatogenic germline and packaged into sperm. PEEL-1::GFP appears to localize to fibrous body-membranous organelles. PEEL-1::GFP does not rescue peel-1(qq99). Reference: Seidel HS, et al. PLoS Biol. 2011 Jul;9(7):e1001115. Mutagen: Outcrossed: x0 Made by: Ailion & Seidel Received: 07/26/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: EG5801 Species: Caenorhabditis elegans Genotype: oxSi87 II; unc-119(ed3) III. Description: oxSi87 [peel-1p::N-terminal 12 amino acids of PEEL-1::GFP::peel-1 3'UTR + Cbr-unc-119(+)] II. GFP is expressed in the spermatogenic germline. During spermatogenesis, GFP is packaged into sperm. Reference: Seidel HS, et al. PLoS Biol. 2011 Jul;9(7):e1001115. Mutagen: Outcrossed: x0 Made by: Ailion & Seidel Received: 07/26/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: EG5897 Species: Caenorhabditis elegans Genotype: oxSi120 II; unc-119(ed3) III. Description: oxSi120 [peel-1p::tagRFP::msp-142 3'UTR + unc-119(+)]. Reference: Batchelder EL, et al. Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11429-34. Mutagen: Mos1 Outcrossed: x Made by: Erik Jorgensen Received: 08/19/11 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: EG5942 Species: Caenorhabditis imperialis Genotype: Caenorhabditis imperialis wild isolate. Description: Caenorhabditis sp. 14 Male-female strain. Made by inbreeding EG5716 for 30 generations by picking a single L4 female and male at each generation. Mutagen: Outcrossed: x Made by: Michael Ailion Received: 12/12/12 University of Washington ----------------------------------------------------------------------------- Strain: EG6032 Species: Caenorhabditis elegans Genotype: ttTi4348 I; unc-18(md299) X. Description: Unc. MosSCI insertion strain with unc-18 marker instead of unc-119. Mos1 insertion in Chr I. Compatible with mosSCI targeting vectors pCFJ448 (Gateway) and pCFJ676 (MCS). Mutagen: MosSCI Outcrossed: x0 Made by: Eric Bend Received: 05/03/12 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG6053 Species: Caenorhabditis elegans Genotype: oxSi212 II; unc-119(ed3) III. Description: oxSi212 [pie-1p::GFP::mCherry::H2B::gld-2 3'UTR::operonGFP::H2B::cye-1UTR] II. Maintain under normal conditions; expression is stable. Superficially wildtype. Bright, nuclear mCherry and GFP fluorescence in germline. Reference: Frokjaer-Jensen C, et al. Nat Methods. 2012 Jan 30;9(2):117-8. Mutagen: Mos1 Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 02/07/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG6070 Species: Caenorhabditis elegans Genotype: oxSi221 II; unc-119(ed3) III. Description: oxSi221 [eft-3p::GFP + Cbr-unc-119(+)] II. Broad, bright GFP fluorescence clearly visible on dissection scope. Single copy insert into MosSCI site ttTi5605 on Chr. II. Can be used as balancer. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 03/23/12 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG6109 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxSi230 X. Description: oxSi230 [eft-3p::GFP + Cbr-unc-119(+)] X. Broad, bright GFP fluorescence. Clearly visible on dissection scope. Single copy insert into MosSCI site ttTi14024 on Chr. X. Can be used as balancer. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 03/23/12 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG6142 Species: Caenorhabditis yunquensis Genotype: Caenorhabditis yunquensis wild isolate. Description: Caenorhabditis sp. 19 Male-female strain. Isolated from a rotten fruit/seed of Ausubo (Manilkara dentata) collected by Susan Dalton in El Yunque, Puerto Rico, near El Verde (~18.3°N, 65.8°W), around March 28, 2010. Mutagen: Outcrossed: x Made by: S Dalton & M Ailion Received: 10/30/12 University of Washington ----------------------------------------------------------------------------- Strain: EG6171 Species: Caenorhabditis elegans Genotype: oxSi257 I; unc-119(ed3) III. Description: oxSi257 [eft-3p::GFP + Cbr-unc-119(+)] I. Broad, bright GFP fluorescence. Clearly visible on dissection scope. Single copy insert into MosSCI site ttTi4391 on Chr. I. Can be used as balancer. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 03/23/12 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG6173 Species: Caenorhabditis elegans Genotype: oxSi259 I; unc-119(ed3) III. Description: oxSi259 [eft-3p::GFP + Cbr-unc-119(+)] I. Broad, bright GFP fluorescence. Clearly visible on dissection scope. Single copy insert into MosSCI site ttTi4348 on Chr. I. Can be used as balancer. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 03/23/12 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG6250 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; cxTi10882 IV. Description: Unc. Not caused by cxTi10882. Mos1 allele generated by NemaGENETAG consortium (Laurent Segalat). Grows best on HB101 bacteria. Reference: Frokjaer-Jensen C, et al., Nat Genet. 2008 Nov;40(11):1375-83. Mutagen: Mos1 transposon Outcrossed: x4 Made by: C. Frokjaer-Jensen Received: 10/27/10 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG6401 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxSi346 IV. Description: oxSi346 [eft-3p::GFP + Cbr-unc-119(+)] IV. Broad, bright GFP fluorescence. Clearly visible on dissection scope. Single copy insert into MosSCI site cxTi10816 on Chr. IV. Can be used as balancer. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 03/23/12 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG6629 Species: Caenorhabditis elegans Genotype: oxIs565 II; oxTi80 III; oxSi199 IV. Description: oxIs565 [dpy-30p::frt::mCherry::frt::GFP::H2B + Cbr-unc-119(+)] II. Ubiquitous mCherry expression. Green nuclei after FLP activity. Integration into ttTi5605 mosSCI site (II:0.77). oxTi80 [eft-3p::GFP::H2B::tbb-2 3'UTR + unc-18(+)] III. Nuclear, green fluorescence is broadly expressed (in most cells). Integration into chr. III: 21.21. oxSi199 [unc-57p::tdTomato + unc-119(+)] IV. Synaptic red fluorescence visible on fluorescence dissecting scope. Integration into cxTi10882 mosSCI site (IV:-0.05). Combined fluorescent balancer strain for LG II, LG III and LG IV. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG6699 Species: Caenorhabditis elegans Genotype: ttTi5605 II; unc-119(ed3) III; oxEx1578. Description: oxEx1578 [eft-3p::GFP + Cbr-unc-119(+)]. Pick non-Unc to maintain. Pick Unc to use for injection. [NOTE: New stock received at the CGC 03/21/12. Original stock was reported as no longer segregating Unc.] Mutagen: MosSCI Outcrossed: x4 Made by: C. Frokjaer-Jensen Received: 03/21/12 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG6700 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; cxTi10882 IV; oxEx1579. Description: oxEx1579 [eft-3p::GFP + Cbr-unc-119(+)]. Pick non-Unc to maintain. Pick Unc to use for injection. Mutagen: MosSCI Outcrossed: x4 Made by: C. Frokjaer-Jensen Received: 04/06/11 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG6701 Species: Caenorhabditis elegans Genotype: ttTi4348 I; unc-119(ed3) III; oxEx1580. Description: oxEx1580 [eft-3p::GFP + Cbr-unc-119(+)]. Pick non-Unc to maintain. Pick Unc to use for injection. Mutagen: MosSCI Outcrossed: x4 Made by: C. Frokjaer-Jensen Received: 04/06/11 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG6702 Species: Caenorhabditis elegans Genotype: ttTi4391 I; unc-119(ed3) III; oxEx1581. Description: oxEx1581 [eft-3p::GFP + Cbr-unc-119(+)]. Pick non-Unc to maintain. Pick Unc to use for injection. Mutagen: MosSCI Outcrossed: x4 Made by: C. Frokjaer-Jensen Received: 04/06/11 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG6703 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; cxTi10816 IV; oxEx1582. Description: oxEx1582 [eft-3p::GFP + Cbr-unc-119(+)]. Pick non-Unc to maintain. Pick Unc to use for injection. oxEx1582 array is stable; pick individual animals to obtain Uncs rather than maintaining by chunking. Mutagen: MosSCI Outcrossed: x4 Made by: C. Frokjaer-Jensen Received: 04/06/11 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG6704 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; ttTi44501 X; oxEx1583. Description: oxEx1583 [eft-3p::GFP + Cbr-unc-119(+)]. Pick non-Unc to maintain. Pick Unc to use for injection. Mutagen: MosSCI Outcrossed: x4 Made by: C. Frokjaer-Jensen Received: 04/06/11 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG6705 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; ttTi14024 X; oxEx1584. Description: oxEx1584 [eft-3p::GFP + Cbr-unc-119]. Pick non-Unc to maintain. Pick Unc to use for injection. Mutagen: MosSCI Outcrossed: x4 Made by: C. Frokjaer-Jensen Received: 04/06/11 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG6787 Species: Caenorhabditis elegans Genotype: oxSi487 II; unc-119(ed3) III. Description: oxSi487 [mex-5p::mCherry::H2B::tbb-2 3'UTR::gpd-2 operon::GFP::H2B::cye-1 3'UTR + unc-119(+)] II. MosSCI insertion into ttTi5605 site on Chr II. unc-119 rescue, bright nuclear GFP and nuclear mCherry fluorescence in germline. Mutagen: MosSCI Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 05/03/12 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7212 Species: Caenorhabditis elegans Genotype: oxTi330 III; gaIs283. Description: oxTi330 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. gaIs283 [unc-54p::GFP::H2B, myo-3p::GFP::H2B, col-93p::GFP::H2B, ref-1p::GFP::H2B], unmapped. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Crossed to SD1780. unc-119(ed3) may be in background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/09/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7213 Species: Caenorhabditis elegans Genotype: oxTi331 I; gaIs283. Description: oxTi331 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. gaIs283 [unc-54p::GFP::H2B, myo-3p::GFP::H2B, col-93p::GFP::H2B, ref-1p::GFP::H2B], unmapped. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Crossed to SD1780. unc-119(ed3) may be in background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/09/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7214 Species: Caenorhabditis elegans Genotype: oxTi333 X; gaIs283. Description: oxTi333 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. gaIs283 [unc-54p::GFP::H2B, myo-3p::GFP::H2B, col-93p::GFP::H2B, ref-1p::GFP::H2B], unmapped. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Crossed to SD1780. unc-119(ed3) may be in background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/09/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7215 Species: Caenorhabditis elegans Genotype: oxTi334; gaIs283. Description: oxTi334 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. gaIs283 [unc-54p::GFP::H2B, myo-3p::GFP::H2B, col-93p::GFP::H2B, ref-1p::GFP::H2B], unmapped. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Crossed to SD1780. unc-119(ed3) may be in background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/09/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7216 Species: Caenorhabditis elegans Genotype: oxTi335 X; gaIs283. Description: oxTi335 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. gaIs283 [unc-54p::GFP::H2B, myo-3p::GFP::H2B, col-93p::GFP::H2B, ref-1p::GFP::H2B], unmapped. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Crossed to SD1780. unc-119(ed3) may be in background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/09/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7309 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi417 V. Description: oxTi417 [eft-3p::mCherry::tbb-2 3'UTR + Cbr-unc-119(+)]. Broad, cytoplasmic red fluorescence. pCFJ660 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7340 Species: C. elegans Genotype: oxIs609 X. Description: oxIs609 [acr-5p::GAP-43::GFP + lin-15(+)] X. GFP localized to axonal membranes of acr-5 expressing neurons. acr-5 promoter drives expression of reporter containing 40 aa myristoylation sequence of GAP43 fused to GFP. oxIs609 was generated by X-ray integration of oxEx81in N2 background. Reference: Rawson RL, et al. Curr Biol. 2014 Mar 31;24(7):760-5. doi: 10.1016/j.cub.2014.02.025. PMID: 24631238. Mutagen: X-ray irradiation Outcrossed: x5 Made by: Randi Rawson Received: 04/04/24 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7477 Species: Caenorhabditis elegans Genotype: syIs46 II; unc-119(ed3) III; oxTi483 X. Description: syIs46 [hsp16p::GFP-LacI; dpy-30p::S65TGFP; dpy-20(+)], oxTi483 [256xLacO + Cbr-unc-119(+) + PuroR]. MiniMos plasmid (pCFJ797) with 256x LacO, Cbr-unc-119(+) and PuroR inserted into syIs46 II; unc-119(ed3) III strain derived from PS2958. May still contain ncl-1 (e1865) III, him-5(e1490)V or dpy-20(e1282ts) IV from PS2958. Puro resistance verified. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7478 Species: Caenorhabditis elegans Genotype: syIs46 II; unc-119(ed3) III; oxTi487 X. Description: syIs46 [hsp16p::GFP-LacI; dpy-30p::S65TGFP; dpy-20(+)], oxTi487 [256xLacO + Cbr-unc-119(+) + PuroR]. MiniMos plasmid (pCFJ797) with 256x LacO, Cbr-unc-119(+) and PuroR inserted into syIs46 II; unc-119(ed3) III strain derived from PS2958. May still contain ncl-1 (e1865) III, him-5(e1490)V or dpy-20(e1282ts) IV from PS2958. Puro resistance verified. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7522 Species: Caenorhabditis elegans Genotype: syIs46 II; oxTi467 unc-119(ed3) III. Description: syIs46 [hsp16p::GFP-LacI; dpy-30p::S65TGFP; dpy-20(+)], oxTi467 [256xLacO + Cbr-unc-119(+) + NeoR]. MiniMos plasmid (pCFJ796) with 256x LacO, Cbr-unc-119(+) and NeoR inserted into syIs46 II; unc-119(ed3) III strain derived from PS2958. May still contain ncl-1 (e1865) III, him-5(e1490)V or dpy-20(e1282ts) IV from PS2958. Neo resistance verified. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7523 Species: Caenorhabditis elegans Genotype: oxTi468 I; syIs46 II; unc-119(ed3) III. Description: syIs46 [hsp16p::GFP-LacI; dpy-30p::S65TGFP; dpy-20(+)], oxTi468 [256xLacO + Cbr-unc-119(+) + NeoR]. MiniMos plasmid (pCFJ796) with 256x LacO, Cbr-unc-119(+) and NeoR inserted into syIs46 II; unc-119(ed3) III strain derived from PS2958. May still contain ncl-1 (e1865) III, him-5(e1490)V or dpy-20(e1282ts) IV from PS2958. Neo resistance verified. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7524 Species: Caenorhabditis elegans Genotype: syIs46 II; unc-119(ed3) III; oxTi469 IV. Description: syIs46 [hsp16p::GFP-LacI; dpy-30p::S65TGFP; dpy-20(+)], oxTi469 [256xLacO + Cbr-unc-119(+) + NeoR]. MiniMos plasmid (pCFJ796) with 256x LacO, Cbr-unc-119(+) and NeoR inserted into syIs46 II; unc-119(ed3) III strain derived from PS2958. May still contain ncl-1 (e1865) III, him-5(e1490)V or dpy-20(e1282ts) IV from PS2958. Neo resistance verified. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7525 Species: Caenorhabditis elegans Genotype: oxTi470 syIs46 II; unc-119(ed3) III. Description: syIs46 [hsp16p::GFP-LacI; dpy-30p::S65TGFP; dpy-20(+)], oxTi470 [256xLacO + Cbr-unc-119(+) + PuroR]. MiniMos plasmid (pCFJ797) with 256x LacO, Cbr-unc-119(+) and PuroR inserted into syIs46 II; unc-119(ed3) III strain derived from PS2958. May still contain ncl-1 (e1865) III, him-5(e1490)V or dpy-20(e1282ts) IV from PS2958. Puro resistance verified. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7526 Species: Caenorhabditis elegans Genotype: oxTi471 syIs46 II; unc-119(ed3) III. Description: syIs46 [hsp16p::GFP-LacI; dpy-30p::S65TGFP; dpy-20(+)], oxTi471 [256xLacO + Cbr-unc-119(+) + PuroR]. MiniMos plasmid (pCFJ797) with 256x LacO, Cbr-unc-119(+) and PuroR inserted into syIs46 II; unc-119(ed3) III strain derived from PS2958. May still contain ncl-1 (e1865) III, him-5(e1490)V or dpy-20(e1282ts) IV from PS2958. Puro resistance verified. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7527 Species: Caenorhabditis elegans Genotype: oxTi472 syIs46 II; unc-119(ed3) III. Description: syIs46 [hsp16p::GFP-LacI; dpy-30p::S65TGFP; dpy-20(+)], oxTi472 [256xLacO + Cbr-unc-119(+) + PuroR]. MiniMos plasmid (pCFJ797) with 256x LacO, Cbr-unc-119(+) and PuroR inserted into syIs46 II; unc-119(ed3) III strain derived from PS2958. May still contain ncl-1 (e1865) III, him-5(e1490)V or dpy-20(e1282ts) IV from PS2958. Puro resistance verified. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7528 Species: Caenorhabditis elegans Genotype: oxTi473 syIs46 II; unc-119(ed3) III. Description: syIs46 [hsp16p::GFP-LacI; dpy-30p::S65TGFP; dpy-20(+)], oxTi473 [256xLacO + Cbr-unc-119(+) + PuroR]. MiniMos plasmid (pCFJ797) with 256x LacO, Cbr-unc-119(+) and PuroR inserted into syIs46 II; unc-119(ed3) III strain derived from PS2958. May still contain ncl-1 (e1865) III, him-5(e1490)V or dpy-20(e1282ts) IV from PS2958. Puro resistance verified. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7529 Species: Caenorhabditis elegans Genotype: syIs46 II; unc-119(ed3) III; oxTi474 V. Description: syIs46 [hsp16p::GFP-LacI; dpy-30p::S65TGFP; dpy-20(+)], oxTi474 [256xLacO + Cbr-unc-119(+) + PuroR]. MiniMos plasmid (pCFJ797) with 256x LacO, Cbr-unc-119(+) and PuroR inserted into syIs46 II; unc-119(ed3) III strain derived from PS2958. May still contain ncl-1 (e1865) III, him-5(e1490) V or dpy-20(e1282ts) IV from PS2958. Puro resistance verified. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7530 Species: Caenorhabditis elegans Genotype: syIs46 II; unc-119(ed3) III; oxTi475 V. Description: syIs46 [hsp16p::GFP-LacI; dpy-30p::S65TGFP; dpy-20(+)], oxTi475 [256xLacO + Cbr-unc-119(+) + PuroR]. MiniMos plasmid (pCFJ797) with 256x LacO, Cbr-unc-119(+) and PuroR inserted into syIs46 II; unc-119(ed3) III strain derived from PS2958. May still contain ncl-1 (e1865) III, him-5(e1490)V or dpy-20(e1282ts) IV from PS2958. Puro resistance verified. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7531 Species: Caenorhabditis elegans Genotype: syIs46 II; unc-119(ed3) III; oxTi476 V. Description: syIs46 [hsp16p::GFP-LacI; dpy-30p::S65TGFP; dpy-20(+)], oxTi476 [256xLacO + Cbr-unc-119(+) + PuroR]. MiniMos plasmid (pCFJ797) with 256x LacO, Cbr-unc-119(+) and PuroR inserted into syIs46 II; unc-119(ed3) III strain derived from PS2958. May still contain ncl-1 (e1865) III, him-5(e1490)V or dpy-20(e1282ts) IV from PS2958. Puro resistance verified. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7532 Species: Caenorhabditis elegans Genotype: syIs46 II; unc-119(ed3) III; oxTi477 IV. Description: syIs46 [hsp16p::GFP-LacI; dpy-30p::S65TGFP; dpy-20(+)], oxTi477 [256xLacO + Cbr-unc-119(+) + PuroR]. MiniMos plasmid (pCFJ797) with 256x LacO, Cbr-unc-119(+) and PuroR inserted into syIs46 II; unc-119(ed3) III strain derived from PS2958. May still contain ncl-1 (e1865) III, him-5(e1490)V or dpy-20(e1282ts) IV from PS2958. Puro resistance verified. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7533 Species: Caenorhabditis elegans Genotype: syIs46 II; unc-119(ed3) III; oxTi478 V. Description: syIs46 [hsp16p::GFP-LacI; dpy-30p::S65TGFP; dpy-20(+)], oxTi478 [256xLacO + Cbr-unc-119(+) + PuroR]. MiniMos plasmid (pCFJ797) with 256x LacO, Cbr-unc-119(+) and PuroR inserted into syIs46 II; unc-119(ed3) III strain derived from PS2958. May still contain ncl-1 (e1865) III, him-5(e1490)V or dpy-20(e1282ts) IV from PS2958. Puro resistance verified. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7534 Species: Caenorhabditis elegans Genotype: oxTi480 syIs46 II; unc-119(ed3) III. Description: syIs46 [hsp16p::GFP-LacI; dpy-30p::S65TGFP; dpy-20(+)], oxTi480 [256xLacO + Cbr-unc-119(+) + PuroR]. MiniMos plasmid (pCFJ797) with 256x LacO, Cbr-unc-119(+) and PuroR inserted into syIs46 II; unc-119(ed3) III strain derived from PS2958. May still contain ncl-1 (e1865) III, him-5(e1490)V or dpy-20(e1282ts) IV from PS2958. Puro resistance verified. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7535 Species: Caenorhabditis elegans Genotype: oxTi481 I; syIs46 II; unc-119(ed3) III. Description: syIs46 [hsp16p::GFP-LacI; dpy-30p::S65TGFP; dpy-20(+)], oxTi481 [256xLacO + Cbr-unc-119(+) + PuroR]. MiniMos plasmid (pCFJ797) with 256x LacO, Cbr-unc-119(+) and PuroR inserted into syIs46 II; unc-119(ed3) III strain derived from PS2958. May still contain ncl-1 (e1865) III, him-5(e1490)V or dpy-20(e1282ts) IV from PS2958. Puro resistance verified. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7536 Species: Caenorhabditis elegans Genotype: syIs46 II; unc-119(ed3) III; oxTi482 IV. Description: syIs46 [hsp16p::GFP-LacI; dpy-30p::S65TGFP; dpy-20(+)], oxTi482 [256xLacO + Cbr-unc-119(+) + PuroR]. MiniMos plasmid (pCFJ797) with 256x LacO, Cbr-unc-119(+) and PuroR inserted into syIs46 II; unc-119(ed3) III strain derived from PS2958. May still contain ncl-1 (e1865) III, him-5(e1490)V or dpy-20(e1282ts) IV from PS2958. Puro resistance verified. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7538 Species: Caenorhabditis elegans Genotype: syIs46 II; unc-119(ed3) oxTi485 III. Description: syIs46 [hsp16p::GFP-LacI; dpy-30p::S65TGFP; dpy-20(+)], oxTi485 [256xLacO + Cbr-unc-119(+) + PuroR]. MiniMos plasmid (pCFJ797) with 256x LacO, Cbr-unc-119(+) and PuroR inserted into syIs46 II; unc-119(ed3) III strain derived from PS2958. May still contain ncl-1 (e1865) III, him-5(e1490)V or dpy-20(e1282ts) IV from PS2958. Puro resistance verified. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7539 Species: Caenorhabditis elegans Genotype: syIs46 II; unc-119(ed3) III; oxTi486 V. Description: syIs46 [hsp16p::GFP-LacI; dpy-30p::S65TGFP; dpy-20(+)], oxTi486 [256xLacO + Cbr-unc-119(+) + PuroR]. MiniMos plasmid (pCFJ797) with 256x LacO, Cbr-unc-119(+) and PuroR inserted into syIs46 II; unc-119(ed3) III strain derived from PS2958. May still contain ncl-1 (e1865) III, him-5(e1490)V or dpy-20(e1282ts) IV from PS2958. Puro resistance verified. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7541 Species: Caenorhabditis elegans Genotype: oxTi479 I; syIs46 II; unc-119(ed3) III. Description: syIs46 [hsp16p::GFP-LacI; dpy-30p::S65TGFP; dpy-20(+)], oxTi479 [256xLacO + Cbr-unc-119(+) + PuroR]. MiniMos plasmid (pCFJ797) with 256x LacO, Cbr-unc-119(+) and PuroR inserted into syIs46 II; unc-119(ed3) III strain derived from PS2958. May still contain ncl-1 (e1865) III, him-5(e1490)V or dpy-20(e1282ts) IV from PS2958. Puro resistance verified. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7565 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi392 V. Description: oxTi392 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7566 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi211 V. Description: oxTi211 [eft-3p::GFP::unc-54 3'UTR + hsp::peel-1 + NeoR + Cbr-unc-119(+)]. Broad, cytoplasmic green fluorescence. pCFJ708 inserted into unc-119(ed3) III (11X outcross) background. Heat-shock inducible negative selection co-inserted (hsp::peel-1). NeoR selection co-inserted. Can be used for positive and negative selection against insertion. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/09/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7799 Species: C. elegans Genotype: unc-119(ed3) III; oxTi374 V; oxEx1873. Description: oxTi374 [unc-18(+) + ttTi5605 NeoR] V. oxEx1873 [Cbr-unc-119(+)]. Pick wild-type to maintain. oxTi374 is a Mini-Mos insertion of ttTi5605 MosSCI landing site in repressive region at position 3,339,184 of Chr V; can be used with standard ttTi5605 mosSCI targeting vectors. Animals carrying the array are wild-type and segregate Unc animals that can be used for MosSCI injections. This strain carries a rescuing unc-119(+) array for easier maintenance; inject Unc-119 animals that have lost the array. Reference: Froekjaer-Jensen et al. Cell (2016). Mutagen: Mos1 element Outcrossed: x5 Made by: Christian Froekjaer-Jensen Received: 06/08/18 King Abduallah University of Science and Technology (KAUST) ----------------------------------------------------------------------------- Strain: EG7803 Species: C. elegans Genotype: unc-119(ed3) III; oxTi176 V; oxEx1807. Description: oxTi176 [unc-18(+) + ttTi5605 NeoR] V. oxEx1807 [Cbr-unc-119(+)]. Pick wild-type to maintain. oxTi176 is a Mini-Mos insertion of ttTi5605 MosSCI landing site in a generally permissive region at position 15,383,969 of Chr V; can be used with standard ttTi5605 mosSCI targeting vectors. Animals carrying the array are wild-type and segregate Unc animals that can be used for MosSCI injections. This strain carries a rescuing unc-119(+) array for easier maintenance; inject Unc-119 animals that have lost the array. Reference: Froekjaer-Jensen et al. Cell (2016). Mutagen: Mos1 Outcrossed: x5 Made by: Christian Froekjaer-Jensen Received: 06/08/18 King Abduallah University of Science and Technology (KAUST) ----------------------------------------------------------------------------- Strain: EG7804 Species: C. elegans Genotype: unc-119(ed3) III; oxTi173 V; oxEx1795. Description: oxTi173 [unc-18(+) + ttTi5605 NeoR] V. oxEx1795 [Cbr-unc-119(+)]. Pick wild-type to maintain. oxTi173 is a Mini-Mos insertion of ttTi5605 MosSCI landing site in a repressive region at position 17,523,246 of Chr V; can be used with standard ttTi5605 MosSCI targeting vectors. Animals carrying the array are wild-type and segregate Unc animals that can be used for MosSCI injections. This strain carries a rescuing unc-119(+) array for easier maintenance; inject Unc-119 animals that have lost the array. Reference: Froekjaer-Jensen et al. Cell (2016). Mutagen: Mos1 Outcrossed: x5 Made by: Christian Froekjaer-Jensen Received: 06/08/18 King Abduallah University of Science and Technology (KAUST) ----------------------------------------------------------------------------- Strain: EG7805 Species: C. elegans Genotype: unc-119(ed3) III; oxTi357 V ; oxEx1876. Description: oxTi357 [unc-18(+) + ttTi5605 NeoR] V. oxEx1876 [Cbr-unc-119(+)]. Pick wild-type to maintain. oxTi357 is a Mini-Mos insertion of ttTi5605 mosSCI landing site in a repressive region at position 20,921,413 of Chr V; can be used with standard ttTi5605 MosSCI targeting vectors. Animals carrying the array are wild-type and segregate Unc animals that can be used for MosSCI injections. This strain carries a rescuing unc-119(+) array for easier maintenance; inject Unc-119 animals that have lost the array. Reference: Froekjaer-Jensen et al. Cell (2016). Mutagen: Mos1 Outcrossed: x5 Made by: Christian Froekjaer-Jensen Received: 06/08/18 King Abduallah University of Science and Technology (KAUST) ----------------------------------------------------------------------------- Strain: EG7826 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi308 X. Description: oxTi308 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/09/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7828 Species: Caenorhabditis elegans Genotype: oxTi310 II; unc-119(ed3) III. Description: oxTi310 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/09/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7831 Species: Caenorhabditis elegans Genotype: oxTi648 I; unc-119(ed3) III. Description: oxTi648 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7832 Species: Caenorhabditis elegans Genotype: oxTi638 I; unc-119(ed3) III. Description: oxTi638 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7833 Species: Caenorhabditis elegans Genotype: oxTi559 I; unc-119(ed3) III. Description: oxTi559 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background at Chr. I:-4.48. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 12/03/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7835 Species: Caenorhabditis elegans Genotype: oxTi556 I; unc-119(ed3) III. Description: oxTi556 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background at Chr. I:1.23. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7836 Species: Caenorhabditis elegans Genotype: oxTi587 I; unc-119(ed3) III. Description: oxTi587 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background at Chr. I:1.28. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7837 Species: Caenorhabditis elegans Genotype: oxTi712 I; unc-119(ed3) III. Description: oxTi712 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background at Chr. I:1.29. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7838 Species: Caenorhabditis elegans Genotype: oxTi718 I; unc-119(ed3) III. Description: oxTi718 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background at Chr. I:2.07. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7839 Species: Caenorhabditis elegans Genotype: oxTi623 I; unc-119(ed3) III. Description: oxTi623 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background at Chr. I:2.75. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7840 Species: Caenorhabditis elegans Genotype: oxTi590 I; unc-119(ed3) III. Description: oxTi590 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background at Chr. I:3.35. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7841 Species: Caenorhabditis elegans Genotype: oxTi302 I; unc-119(ed3) III. Description: oxTi302 [eft-3p::mCherry::tbb-2 3'UTR + Cbr-unc-119(+)]. Broad, cytoplasmic red fluorescence. pCFJ660 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/09/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7842 Species: Caenorhabditis elegans Genotype: oxTi653 I; unc-119(ed3) III. Description: oxTi653 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7843 Species: Caenorhabditis elegans Genotype: oxTi398 I; unc-119(ed3) III. Description: oxTi398 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background at Chr. I:14.26. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/09/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7844 Species: Caenorhabditis elegans Genotype: oxTi413 I; unc-119(ed3) III. Description: oxTi413 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background at Chr. I:17.17. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7845 Species: Caenorhabditis elegans Genotype: oxTi571 I; unc-119(ed3) III. Description: oxTi571 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background at Chr. I:21.62. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7846 Species: Caenorhabditis elegans Genotype: oxTi700 I; unc-119(ed3) III. Description: oxTi700 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background at Chr.I:22.30. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7847 Species: Caenorhabditis elegans Genotype: oxTi723 I; unc-119(ed3) III. Description: oxTi723 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background at Chr.I:25.79. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7848 Species: Caenorhabditis elegans Genotype: oxTi626 I; unc-119(ed3) III. Description: oxTi626 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background at Chr.I:29.18. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7851 Species: Caenorhabditis elegans Genotype: oxTi732 I; unc-119(ed3) III. Description: oxTi732 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background at Chr.I:29.97. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7854 Species: Caenorhabditis elegans Genotype: oxTi730 I; unc-119(ed3) III. Description: oxTi730 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7855 Species: Caenorhabditis elegans Genotype: oxTi724 II; unc-119(ed3) III. Description: oxTi724 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7856 Species: Caenorhabditis elegans Genotype: oxTi624 II; unc-119(ed3) III. Description: oxTi624 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7857 Species: Caenorhabditis elegans Genotype: oxTi628 II; unc-119(ed3) III. Description: oxTi628 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7858 Species: Caenorhabditis elegans Genotype: oxTi729 II; unc-119(ed3) III. Description: oxTi729 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7859 Species: Caenorhabditis elegans Genotype: oxTi402 II; unc-119(ed3) III. Description: oxTi402 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/09/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7860 Species: Caenorhabditis elegans Genotype: oxTi677 II; unc-119(ed3) III. Description: oxTi677 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background at ChrII:-12.17. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7862 Species: Caenorhabditis elegans Genotype: oxTi647 II; unc-119(ed3) III. Description: oxTi647 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7863 Species: Caenorhabditis elegans Genotype: oxTi717 II; unc-119(ed3) III. Description: oxTi717 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background at ChrII:-4.73. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7864 Species: Caenorhabditis elegans Genotype: oxTi726 II; unc-119(ed3) III. Description: oxTi726 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background at ChrII:-1.02. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7865 Species: Caenorhabditis elegans Genotype: oxTi617 II; unc-119(ed3) III. Description: oxTi617 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7866 Species: Caenorhabditis elegans Genotype: oxTi564 II; unc-119(ed3) III. Description: oxTi564 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7867 Species: Caenorhabditis elegans Genotype: oxTi394 II; unc-119(ed3) III. Description: oxTi394 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7868 Species: Caenorhabditis elegans Genotype: oxTi629 II; unc-119(ed3) III. Description: oxTi629 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7869 Species: Caenorhabditis elegans Genotype: oxTi582 II; unc-119(ed3) III. Description: oxTi582 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7870 Species: Caenorhabditis elegans Genotype: oxTi540 II; unc-119(ed3) III. Description: oxTi540 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7871 Species: Caenorhabditis elegans Genotype: oxTi548 II; unc-119(ed3) III. Description: oxTi548 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7872 Species: Caenorhabditis elegans Genotype: oxTi573 II; unc-119(ed3) III. Description: oxTi573 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7873 Species: Caenorhabditis elegans Genotype: oxTi609 II; unc-119(ed3) III. Description: oxTi609 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7874 Species: Caenorhabditis elegans Genotype: oxTi714 II; unc-119(ed3) III. Description: oxTi714 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7875 Species: Caenorhabditis elegans Genotype: oxTi574 II; unc-119(ed3) III. Description: oxTi574 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7876 Species: Caenorhabditis elegans Genotype: oxTi722 II; unc-119(ed3) III. Description: oxTi722 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7877 Species: Caenorhabditis elegans Genotype: oxTi583 II; unc-119(ed3) III. Description: oxTi583 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7878 Species: Caenorhabditis elegans Genotype: oxTi720 II; unc-119(ed3) III. Description: oxTi720 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7879 Species: Caenorhabditis elegans Genotype: oxTi296 II; unc-119(ed3) III. Description: oxTi296 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/09/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7880 Species: Caenorhabditis elegans Genotype: oxTi589 II; unc-119(ed3) III. Description: oxTi589 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7881 Species: Caenorhabditis elegans Genotype: oxTi409 II; unc-119(ed3) III; him-5(e1490) V. Description: oxTi409 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Him. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III; him-5(e1490) V background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7882 Species: Caenorhabditis elegans Genotype: oxTi657 II; unc-119(ed3) III. Description: oxTi657 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7884 Species: Caenorhabditis elegans Genotype: oxTi607 II; unc-119(ed3) III. Description: oxTi607 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7885 Species: Caenorhabditis elegans Genotype: oxTi721 II; unc-119(ed3) III. Description: oxTi721 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7886 Species: Caenorhabditis elegans Genotype: oxTi399 II; unc-119(ed3) III. Description: oxTi399 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7887 Species: Caenorhabditis elegans Genotype: oxTi731 unc-119(ed3) III. Description: oxTi731 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7888 Species: Caenorhabditis elegans Genotype: oxTi641 unc-119(ed3) III. Description: oxTi641 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7890 Species: Caenorhabditis elegans Genotype: oxTi706 unc-119(ed3) III. Description: oxTi706 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7891 Species: Caenorhabditis elegans Genotype: oxTi544 unc-119(ed3) III. Description: oxTi544 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7892 Species: Caenorhabditis elegans Genotype: oxTi612 unc-119(ed3) III. Description: oxTi612 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7893 Species: Caenorhabditis elegans Genotype: oxTi615 unc-119(ed3) III. Description: oxTi615 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7894 Species: Caenorhabditis elegans Genotype: oxTi603 unc-119(ed3) III. Description: oxTi603 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7895 Species: Caenorhabditis elegans Genotype: oxTi411 unc-119(ed3) III. Description: oxTi411 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7896 Species: Caenorhabditis elegans Genotype: oxTi627 unc-119(ed3) III. Description: oxTi627 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7897 Species: Caenorhabditis elegans Genotype: oxTi719 unc-119(ed3) III. Description: oxTi719 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7898 Species: Caenorhabditis elegans Genotype: oxTi619 unc-119(ed3) III. Description: oxTi619 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7899 Species: Caenorhabditis elegans Genotype: unc-119(ed3) oxTi734 III. Description: oxTi734 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7900 Species: Caenorhabditis elegans Genotype: unc-119(ed3) oxTi546 III. Description: oxTi546 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7901 Species: Caenorhabditis elegans Genotype: unc-119(ed3) oxTi539 III. Description: oxTi539 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7902 Species: Caenorhabditis elegans Genotype: unc-119(ed3) oxTi713 III. Description: oxTi713 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7903 Species: Caenorhabditis elegans Genotype: unc-119(ed3) oxTi715 III. Description: oxTi715 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7904 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi716 IV. Description: oxTi716 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7905 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi390 IV. Description: oxTi390 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/09/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7906 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi608 IV. Description: oxTi608 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7907 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi408 IV; him-5(e1490) V. Description: oxTi408 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Him. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III; him-5(e1490) V background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/09/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7908 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi636 IV. Description: oxTi636 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7909 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi670 IV. Description: oxTi670 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7910 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi420 IV. Description: oxTi420 [eft-3p::mCherry::tbb-2 3'UTR + Cbr-unc-119(+)]. Broad, cytoplasmic red fluorescence. pCFJ660 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7911 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi705 IV. Description: oxTi705 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7912 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi397 IV. Description: oxTi397 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7913 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi563 IV. Description: oxTi563 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7914 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi622 IV. Description: oxTi622 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7915 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi419 IV. Description: oxTi419 [eft-3p::mCherry::tbb-2 3'UTR + Cbr-unc-119(+)]. Broad, cytoplasmic red fluorescence. pCFJ660 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/09/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7916 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi208 IV. Description: oxTi208 [eft-3p::GFP::unc-54 3'UTR + hsp::peel-1 + NeoR + Cbr-unc-119(+)]. Broad, cytoplasmic green fluorescence. pCFJ708 inserted into unc-119(ed3) III (11X outcross) background. Heat-shock inducible negative selection co-inserted (hsp::peel-1). NeoR selection co-inserted. Can be used for positive and negative selection against insertion. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/09/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7917 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi616 IV. Description: oxTi616 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7918 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi727 IV. Description: oxTi727 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7919 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi584 IV. Description: oxTi584 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7920 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi551 IV. Description: oxTi551 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. [NOTE (8-17-2016) One researcher has noticed an unusual segregation pattern of the oxTi551 insertion. This strain may contain more than one insertion or is possible mis-mapped - the authors recommend that you verify the location of this insertion prior to using it as a genetic marker.] Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7921 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi414 IV. Description: oxTi414 [eft-3p::mCherry::tbb-2 3'UTR + Cbr-unc-119(+)]. Broad, cytoplasmic red fluorescence. pCFJ660 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7922 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi537 IV. Description: oxTi537 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7923 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi552 IV. Description: oxTi552 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7924 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi599 IV. Description: oxTi599 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7925 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi611 IV. Description: oxTi611 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7926 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi418 IV. Description: oxTi418 [eft-3p::mCherry::tbb-2 3'UTR + Cbr-unc-119(+)]. Broad, cytoplasmic red fluorescence. pCFJ660 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7927 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi404 IV. Description: oxTi404 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7928 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi407 IV; him-5(e1490) V. Description: oxTi407 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Him. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III; him-5(e1490) V background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/09/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7929 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi602 IV. Description: oxTi602 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7930 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi538 IV. Description: oxTi538 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7931 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi577 IV. Description: oxTi577 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7932 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi592 IV. Description: oxTi592 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7933 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi391 IV. Description: oxTi391 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7934 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi554 IV. Description: oxTi554 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7935 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi581 IV. Description: oxTi581 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7936 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi664 V. Description: oxTi664 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7938 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi640 V. Description: oxTi640 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7939 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi654 V. Description: oxTi654 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7940 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi708 V. Description: oxTi708 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7941 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi396 V. Description: oxTi396 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7942 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi393 V. Description: oxTi393 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7943 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi703 V. Description: oxTi703 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7944 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi553 V. Description: oxTi553 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7945 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi543 V. Description: oxTi543 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7946 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi580 V. Description: oxTi580 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7947 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi606 V. Description: oxTi606 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7948 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi620 V. Description: oxTi620 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7949 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi635 V. Description: oxTi635 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7950 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi711 V. Description: oxTi711 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7951 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi630 V. Description: oxTi630 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7952 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi207 V. Description: oxTi207 [eft-3p::GFP::unc-54 3'UTR + hsp::peel-1 + NeoR + Cbr-unc-119(+)]. Broad, cytoplasmic green fluorescence. pCFJ708 inserted into unc-119(ed3) III (11X outcross) background. Heat-shock inducible negative selection co-inserted (hsp::peel-1). NeoR selection co-inserted. Can be used for positive and negative selection against insertion. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/09/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7953 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi542 V. Description: oxTi542 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7954 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi575 V. Description: oxTi575 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7955 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi725 V. Description: oxTi725 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7956 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi547 V. Description: oxTi547 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7957 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi389 V. Description: oxTi389 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7958 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi710 V. Description: oxTi710 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7959 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; him-5(e1490) oxTi405 V. Description: oxTi405 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)] V. Maintain at 20C; somewhat sick at 25C. Him. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III; him-5(e1490) V background at Chr. V:8.97. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7960 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi401 V. Description: oxTi401 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/09/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7968 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi633 V. Description: oxTi633 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7969 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi621 X. Description: oxTi621 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7970 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi597 X. Description: oxTi597 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7971 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; him-5(e1490) V; oxTi406 X. Description: oxTi406 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Him. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III; him-5(e1490) V background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7972 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi673 X. Description: oxTi673 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7975 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi651 X. Description: oxTi651 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7976 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi702 X. Description: oxTi702 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7977 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi410 X. Description: oxTi410 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7978 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi675 X. Description: oxTi675 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7980 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi557 X. Description: oxTi557 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7981 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi639 X. Description: oxTi639 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7982 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi728 X. Description: oxTi728 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7983 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi595 X. Description: oxTi595 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7984 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi637 X. Description: oxTi637 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7985 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi566 X. Description: oxTi566 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7986 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi421 X. Description: oxTi421 [eft-3p::mCherry::tbb-2 3'UTR + Cbr-unc-119(+)]. Broad, cytoplasmic red fluorescence. pCFJ660 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7987 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi416 X. Description: oxTi416 [eft-3p::mCherry::tbb-2 3'UTR + Cbr-unc-119(+)]. Broad, cytoplasmic red fluorescence. pCFJ660 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7988 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi704 X. Description: oxTi704 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7989 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi668 X. Description: oxTi668 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7990 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi400 X. Description: oxTi400 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7991 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi594 X. Description: oxTi594 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7992 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi545 X. Description: oxTi545 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7993 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi412 X. Description: oxTi412 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7994 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi395 X. Description: oxTi395 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/09/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7995 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi596 X. Description: oxTi596 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7996 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi403 X. Description: oxTi403 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7998 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi709 X. Description: oxTi709 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG7999 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi649 X. Description: oxTi649 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8000 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi659 X. Description: oxTi659 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8001 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi701 X. Description: oxTi701 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8003 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi707 X. Description: oxTi707 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8004 Species: Caenorhabditis elegans Genotype: oxTi550 I; unc-119(ed3) III. Description: oxTi550 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]. Maintain at 20C; somewhat sick at 25C. Broad, nuclear red fluorescence. pCFJ453 inserted into unc-119(ed3) III (11X outcross) background at Chr. I:-18.63. Please see www.wormbuilder.org for exact insertion site. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 04/15/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8040 Species: Caenorhabditis elegans Genotype: oxTi302 I; oxTi75 II; oxTi411 unc-119(ed3) III; him-8(e1489) IV. Description: oxTi302 [eft-3p::mCherry::tbb-2 3'UTR + Cbr-unc-119(+)]; inserted in Chr. I: 10,166,145. oxTi75 [eft-3p::GFP::H2B::tbb-2 3'UTR + unc-18(+)] II; inserted in Chr. II: 5,448,544. oxTi411 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)] inserted in Chr. III: 6,076,837. Him. Somewhat sick at higher temperatures. Maintain at 20C or below. This mapping strain uses three dominant fluorescent insertions that can be distinguished when scoring segregation. Please see www.wormbuilder.org for additional strain information. Mutagen: Mos1 Outcrossed: x0 Made by: Matthew LaBella Received: 12/02/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8041 Species: Caenorhabditis elegans Genotype: oxTi76 IV; oxTi405 him-5(e1490) V; oxTi421 X. Description: oxTi76 [eft-3p::GFP::H2B::tbb-2 3'UTR + unc-18(+)]; inserted in Chr. IV: 11,899,008. oxTi405 [eft-3p::tdTomato::H2B::unc-54 3'UTR + Cbr-unc-119(+)]; inserted in Chr. V: 15,838,568. oxTi421 [eft-3p::mCherry::tbb-2 3'UTR] inserted in Chr. X: 6,180,397. Him. Somewhat sick at higher temperatures. Maintain at 20C or below. This mapping strain uses three dominant fluorescent insertions that can be distinguished when scoring segregation. Please see www.wormbuilder.org for additional strain information. Mutagen: Mos1 Outcrossed: x0 Made by: Matthew LaBella Received: 12/02/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8072 Species: Caenorhabditis elegans Genotype: oxSi259 I; oxIs322 II; oxTi81 V. Description: oxSi259 [eft-3p::GFP + Cbr-unc-119(+)] I. Cytoplasmic, green fluorescence expressed broadly (most cells). Integration into ttTi4348 mosSCI site (I:-5.32). oxIs322 [myo-2p::mCherry::H2B + myo-3p::mCherry::H2B + ? + Cbr-unc-119(+)] III. Nuclear, red fluorescence in pharynx and body wall muscle. Complex integration into ttTi5605 mosSCI site (II:0.77). oxTi81 [eft-3p::GFP::H2B::tbb-2 3'UTR + unc-18(+)] V. Nuclear, green fluorescence is broadly expressed (in most cells). Integration into chr.V: 1.21. Combined fluorescent balancer strain for LG I, LG II and LG V. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8073 Species: Caenorhabditis elegans Genotype: oxIs322 II; oxSi199 IV; oxTi81 him-5(e1490) V. Description: oxIs322 [myo-2p::mCherry::H2B + myo-3p::mCherry::H2B + ? + Cbr-unc-119(+)] III. Nuclear, red fluorescence in pharynx and body wall muscle. Complex integration into ttTi5605 mosSCI site (II:0.77). oxSi199 [unc-57p::tdTomato + unc-119(+)] IV. Synaptic red fluorescence visible on fluorescence dissecting scope. Integration into cxTi10882 mosSCI site (IV:-0.05). oxTi81 [eft-3p::GFP::H2B::tbb-2 3'UTR + unc-18(+)] V. Nuclear, green fluorescence is broadly expressed (in most cells). Integration into chr.V: 1.21. Him. Combined fluorescent balancer strain for LG II, LG IV and LG V. Strain contains him-5(e1490) to generate males for crosses. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8078 Species: Caenorhabditis elegans Genotype: oxTi185 I; unc-119(ed3) III. Description: oxTi185 [ttTi5605 + NeoR(+) + unc-18(+)]. Unc. Grows best at 20C on HB101. Strain contains a universal MosSCI insertion site that is compatible with targeting vectors for the ttTi5605 site (for example, pCFJ150 derivatives). This site is generally permissive for germline expression. Transgenic animals are NeoR and carry an extra copy of unc-18(+). Please see www.wormbuilder.org for more details. Mutagen: Mos1 transposon Outcrossed: x4 Made by: C. Frokjaer-Jensen Received: 06/14/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8079 Species: Caenorhabditis elegans Genotype: oxTi179 II; unc-119(ed3) III. Description: oxTi179 [ttTi5605 + NeoR(+) + unc-18(+)]. Unc. Grows best at 20C on HB101. Strain contains a universal MosSCI insertion site that is compatible with targeting vectors for the ttTi5605 site (for example, pCFJ150 derivatives). This site is generally permissive for germline expression. Transgenic animals are NeoR and carry an extra copy of unc-18(+). Please see www.wormbuilder.org for more details. Mutagen: Mos1 transposon Outcrossed: x4 Made by: C. Frokjaer-Jensen Received: 06/14/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8080 Species: Caenorhabditis elegans Genotype: oxTi444 unc-119(ed3) III. Description: oxTi444 [ttTi5605 + NeoR(+) + unc-18(+)]. Unc. Grows best at 20C on HB101. Strain contains a universal MosSCI insertion site that is compatible with targeting vectors for the ttTi5605 site (for example, pCFJ150 derivatives). This site is generally permissive for germline expression. Transgenic animals are NeoR and carry an extra copy of unc-18(+). Please see www.wormbuilder.org for more details. Mutagen: Mos1 transposon Outcrossed: x4 Made by: C. Frokjaer-Jensen Received: 06/14/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8081 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi177 IV. Description: oxTi177 [ttTi5605 + NeoR(+) + unc-18(+)]. Unc. Grows best at 20C on HB101. Strain contains a universal MosSCI insertion site that is compatible with targeting vectors for the ttTi5605 site (for example, pCFJ150 derivatives). This site is generally permissive for germline expression. Transgenic animals are NeoR and carry an extra copy of unc-18(+). Please see www.wormbuilder.org for more details. Mutagen: Mos1 transposon Outcrossed: x4 Made by: C. Frokjaer-Jensen Received: 06/14/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8082 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi365 V. Description: oxTi365 [ttTi5605 + NeoR(+) + unc-18(+)]. Unc. Grows best at 20C on HB101. Strain contains a universal MosSCI insertion site that is compatible with targeting vectors for the ttTi5605 site (for example, pCFJ150 derivatives). This site is generally permissive for germline expression. Transgenic animals are NeoR and carry an extra copy of unc-18(+). Please see www.wormbuilder.org for more details. Mutagen: Mos1 transposon Outcrossed: x4 Made by: C. Frokjaer-Jensen Received: 06/14/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8083 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi354 V. Description: oxTi354 [myo-2p::GFP::H2B + ttTi5605 + unc-18(+)]. Unc. Grows best at 20C on HB101. Strain contains a universal MosSCI insertion site that is compatible with targeting vectors for the ttTi5605 site (for example, pCFJ150 derivatives). This site is generally permissive for germline expression. Transgenic animals contain a myo-2p::GFP::H2B construct next to the insertion site and carry an extra copy of unc-18(+). Please see www.wormbuilder.org for more details. Mutagen: Mos1 transposon Outcrossed: x4 Made by: C. Frokjaer-Jensen Received: 06/14/13 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8397 Species: Caenorhabditis elegans Genotype: oxIs322 II; oxTi80 III; him-5(e1490) V. Description: oxIs322 [myo-2p::mCherry::H2B + myo-3p::mCherry::H2B + ? + Cbr-unc-119(+)] III. Nuclear, red fluorescence in pharynx and body wall muscle. Complex integration into ttTi5605 mosSCI site (II:0.77). oxTi80 [eft-3p::GFP::H2B::tbb-2 3'UTR + unc-18(+)] III. Nuclear, green fluorescence is broadly expressed (in most cells). Integration into chr. III: 21.21. Him. Combined fluorescent balancer strain for LG II and LG III. Strain contains him-5(e1490) to generate males for crosses. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8398 Species: Caenorhabditis elegans Genotype: oxIs322 II; oxTi80 III; oxSi199 IV; him-5(e1490) V. Description: oxIs322 [myo-2p::mCherry::H2B + myo-3p::mCherry::H2B + ? + Cbr-unc-119(+)] III. Nuclear, red fluorescence in pharynx and body wall muscle. Complex integration into ttTi5605 mosSCI site (II:0.77). oxTi80 [eft-3p::GFP::H2B::tbb-2 3'UTR + unc-18(+)] III. Nuclear, green fluorescence is broadly expressed (in most cells). Integration into chr. III: 21.21. oxSi199 [unc-57p::tdTomato + unc-119(+)] IV. Synaptic red fluorescence visible on fluorescence dissecting scope. Integration into cxTi10882 mosSCI site (IV:-0.05). Him. Combined fluorescent balancer strain for LG II, LG III and LG IV. Strain contains him-5(e1490) to generate males for crosses. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8760 Species: Caenorhabditis elegans Genotype: oxIs322 II; oxTi79 III; him-5(e1490) V. Description: oxIs322 [myo-2p::mCherry::H2B + myo-3p::mCherry::H2B + ? + Cbr-unc-119(+)] III. Nuclear, red fluorescence in pharynx and body wall muscle. Complex integration into ttTi5605 mosSCI site (II:0.77). oxTi79 [eft-3p::GFP::H2B::tbb-2 3' UTR + unc-18(+)] III. Nuclear, green fluorescence is broadly expressed (in most cells). Integration into chr. III: -26.22. Him. Combined fluorescent balancer strain for LG II and LG III. Strain contains him-5(e1490) to generate males for crosses. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8776 Species: Caenorhabditis elegans Genotype: oxSi255 I; oxIs322 II; oxSi199 IV; him-5(e1490) V. Description: oxSi255 [snt-1p::GFP + Cbr-unc-119(+)] I. Integration into ttTi4348 mosSCI site (I:-5.32). Pan-neuronal GFP expression visible under dissection microscope. oxIs322 [myo-2p::mCherry::H2B + myo-3p::mCherry::H2B + ? + Cbr-unc-119(+)] III. Nuclear, red fluorescence in pharynx and body wall muscle. Complex integration into ttTi5605 mosSCI site (II:0.77). oxSi199 [unc-57p::tdTomato + unc-119(+)] IV. Synaptic red fluorescence visible on fluorescence dissecting scope. Integration into cxTi10882 mosSCI site (IV:-0.05). Him. Combined fluorescent balancer strain for LG I, LG II and LG IV. Strain contains him-5(e1490) to generate males for crosses. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8829 Species: Caenorhabditis elegans Genotype: unc-119(ed3) oxTi872 III. Description: oxTi872 [vha-6p::GFP::tbb-2 3'UTR + Cbr-unc-119(+)] III. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Cytoplasmic green fluorescence in intestinal cells visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (III:-1.41). Insertion into C23G10.7 3'UTR. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1401 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8830 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi873 IV. Description: oxTi873 [vha-6p::GFP::tbb-2 3'UTR + Cbr-unc-119(+)] IV. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Cytoplasmic green fluorescence in intestinal cells visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (IV:6.44). Insertion into ZK896.1. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1401 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8831 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi874 V. Description: oxTi874 [vha-6p::GFP::tbb-2 3'UTR + Cbr-unc-119(+)] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Cytoplasmic green fluorescence in intestinal cells visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:-13.00). Insertion into srh-36. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1401 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8832 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi875 V. Description: oxTi875 [vha-6p::GFP::tbb-2 3'UTR + Cbr-unc-119(+)] V. Strain is healthy. NOTE: High percentage of males observed (unpublished observations, Chee Kiang Ewe, Rothman lab, UCSB). NOTE: This strain is not necessarily homozygous - please verify before using. Cytoplasmic green fluorescence in intestinal cells visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:20.19). Insertion into pro-3 3'UTR. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1401 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8833 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi876 IV. Description: oxTi876 [vha-6p::GFP::tbb-2 3'UTR + Cbr-unc-119(+)] IV. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Cytoplasmic green fluorescence in intestinal cells visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (IV:4.53). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1401 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8834 Species: Caenorhabditis elegans Genotype: oxTi877 II; unc-119(ed3) III Description: oxTi877 [vha-6p::GFP::tbb-2 3'UTR + Cbr-unc-119(+)] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Cytoplasmic green fluorescence in intestinal cells visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:-2.02). Insertion into ggr-3. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1401 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8835 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi878 V. Description: oxTi878 [vha-6p::GFP::tbb-2 3'UTR + Cbr-unc-119(+)] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Cytoplasmic green fluorescence in intestinal cells visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:1.58). Insertion into B0507.6. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1401 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8836 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi879 V. Description: oxTi879 [vha-6p::GFP::tbb-2 3'UTR + Cbr-unc-119(+)] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Cytoplasmic green fluorescence in intestinal cells visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:2.02). Insertion into H24G06.1. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1401 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8837 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi880 IV. Description: oxTi880 [vha-6p::GFP::tbb-2 3'UTR + Cbr-unc-119(+)] IV. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Cytoplasmic green fluorescence in intestinal cells visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (IV:1.82). Insertion into drp-1. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1401 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8838 Species: Caenorhabditis elegans Genotype: oxTi882 II; unc-119(ed3) III. Description: oxTi882 [vha-6p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence in intestinal cells visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:0.13). Insertion into ncRNA C44B7.21. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1405 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8839 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi884 IV. Description: oxTi884 [vha-6p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] IV. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence in intestinal cells visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (IV:3.90). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1405 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8840 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi885 X. Description: oxTi885 [vha-6p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] X. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence in intestinal cells visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (X:9.13). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1405 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8841 Species: Caenorhabditis elegans Genotype: oxTi886 II; unc-119(ed3) III. Description: oxTi886 [myo-3p::GFP::tbb-2 3'UTR + Cbr-unc-119(+)] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Cytoplasmic green fluorescence in body wall muscles visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:-14.32). Insertion into T08E11.8. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1400 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8842 Species: Caenorhabditis elegans Genotype: oxTi887 II; unc-119(ed3) III. Description: oxTi887 [myo-3p::GFP::tbb-2 3'UTR + Cbr-unc-119(+)] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Cytoplasmic green fluorescence in body wall muscles visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:2.59). Insertion into T08E11.8. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1400 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8843 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi888 V. Description: oxTi888 [myo-3p::GFP::tbb-2 3'UTR + Cbr-unc-119(+)] V. Strain is healthy. Pick GFP+ non-Unc to maintain. NOTE: This strain is not necessarily homozygous - please verify before using. Cytoplasmic green fluorescence in body wall muscles visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:3.88). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1400 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8844 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi889 V. Description: oxTi889 [myo-3p::GFP::tbb-2 3'UTR + Cbr-unc-119(+)] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Cytoplasmic green fluorescence in body wall muscles visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:1.44). Insertion into F25G6.7. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1400 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8845 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi890 X. Description: oxTi890 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] X. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (X:-0.43). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8846 Species: Caenorhabditis elegans Genotype: oxTi891 II; unc-119(ed3) III. Description: oxTi891 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:-0.61). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8847 Species: Caenorhabditis elegans Genotype: oxTi892 unc-119(ed3) III. Description: oxTi892 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] III. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (III:-0.93). Insertion into srv-1. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8848 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi893 X. Description: oxTi893 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] X. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (X:0.57). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8849 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi894 V. Description: oxTi894 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:11.54). Insertion into srh-131. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8850 Species: Caenorhabditis elegans Genotype: oxTi895 I; unc-119(ed3) III. Description: oxTi895 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:0.22). Insertion into klp-15. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8851 Species: Caenorhabditis elegans Genotype: oxTi897 unc-119(ed3) III. Description: oxTi897 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] III. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (III:-0.36). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8852 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi898 X. Description: oxTi898 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] X. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (X:24.44). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8853 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi899 X. Description: oxTi899 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] X. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (X:24.63). Insertion into cTel55X. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8854 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi900 X. Description: oxTi900 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] X. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (X:-9.39). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8855 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi901 V. Description: oxTi901 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:19.80). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8856 Species: Caenorhabditis elegans Genotype: oxTi902 I; unc-119(ed3) III. Description: oxTi902 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:-1.50). Insertion into anc-1. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8857 Species: Caenorhabditis elegans Genotype: oxTi903 II; unc-119(ed3) III. Description: oxTi903 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:3.15). Insertion into nlp-18. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8858 Species: Caenorhabditis elegans Genotype: oxTi904 II; unc-119(ed3) III. Description: oxTi904 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:0.50). Insertion into dyf-13. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8859 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi905 X. Description: oxTi905 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] X. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (X:24.08). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8860 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi906 V. Description: oxTi906 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:24.52). Insertion into ttx-1. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8861 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi907 V. Description: oxTi907 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:11.98). Insertion into srz-92 (pseudogene). Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8862 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi908 V. Description: oxTi908 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:12.85). Insertion into sri-70. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8863 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi909 IV. Description: oxTi909 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] IV. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (IV:4.02). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8864 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi910 X. Description: oxTi910 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] X. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (X:24.62). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8865 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi911 V. Description: oxTi911 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:4.95). Insertion into dhc-3. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8866 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi912. Description: oxTi912 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] IV. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (IV:14.22). Intragenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8867 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi914 IV. Description: oxTi914 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] IV. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (IV:-25.76). Insertion into F56A11.7. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8868 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi915 IV. Description: oxTi915 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] IV. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (IV:-5.63). Insertion into puf-4 (pseudogene). Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8869 Species: Caenorhabditis elegans Genotype: oxTi917 I; unc-119(ed3) III. Description: oxTi917 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:8.72). Intragenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8870 Species: Caenorhabditis elegans Genotype: oxTi918 I; unc-119(ed3) III. Description: oxTi918 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:1.67). Intragenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8871 Species: Caenorhabditis elegans Genotype: oxTi919 I; unc-119(ed3) III. Description: oxTi919 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:13.92). Insertion into sra-25. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8872 Species: Caenorhabditis elegans Genotype: oxTi920 I; unc-119(ed3) III. Description: oxTi920 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:1.60). Insertion into C30F12.5. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8873 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi921 IV. Description: oxTi921 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] IV. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (IV:8.85). Insertion into rab-19. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8874 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi922 V. Description: oxTi922 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:8.58). Intragenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8875 Species: Caenorhabditis elegans Genotype: oxTi923 I; unc-119(ed3) III. Description: oxTi923 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:3.44). Insertion into hgo-1. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8876 Species: Caenorhabditis elegans Genotype: oxTi924 II; unc-119(ed3) III. Description: oxTi924 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:22.90). Insertion into srh-42. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8877 Species: Caenorhabditis elegans Genotype: oxTi925 I; unc-119(ed3) III. Description: oxTi925 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:7.93). Insertion into F36D1.8 (promoter). Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8878 Species: Caenorhabditis elegans Genotype: oxTi926 I; unc-119(ed3) III. Description: oxTi926 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:24.67). Intragenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8879 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi927 IV. Description: oxTi927 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] IV. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (IV:-16.25). Intragenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8881 Species: Caenorhabditis elegans Genotype: oxTi929 II; unc-119(ed3) III. Description: oxTi929 [eft-3p::GFP::2xNLS::tbb-2 3'UTR +Cbr-unc-119(+)] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:3.16). Insertion into F33A8.6. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8882 Species: Caenorhabditis elegans Genotype: oxTi930 II; unc-119(ed3) III. Description: oxTi930 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:10.05). Insertion into F15A4.2. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8883 Species: Caenorhabditis elegans Genotype: oxTi931 II; unc-119(ed3) III. Description: oxTi931 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:3.49). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8884 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi932 X. Description: oxTi932 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] X. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (X:24.09). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8885 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi933 V. Description: oxTi933 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:3.38). Insertion into D2023.3. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8886 Species: Caenorhabditis elegans Genotype: oxTi934 I; unc-119(ed3) III. Description: oxTi934 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:-3.79). Insertion into W05F2.6. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8887 Species: Caenorhabditis elegans Genotype: oxTi935 unc-119(ed3) III. Description: oxTi935 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] III. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (III:-3.17). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8888 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi936 X. Description: oxTi936 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] X. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (X:-13.81). Insertion into igcm-3. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8889 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi938 IV. Description: oxTi938 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] IV. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (IV:2.79). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8890 Species: Caenorhabditis elegans Genotype: oxTi939 II; unc-119(ed3) III. Description: oxTi939 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:0.12). Intergenic insertion into snt-1 3' UTR. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8891 Species: Caenorhabditis elegans Genotype: oxTi940 II; unc-119(ed3) III. Description: oxTi940 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:0.56). Insertion into C06A8.8. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8892 Species: Caenorhabditis elegans Genotype: oxTi941 II; unc-119(ed3) III. Description: oxTi941 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:-2.60). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8893 Species: Caenorhabditis elegans Genotype: oxTi943 II; unc-119(ed3) III. Description: oxTi943 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:20.30). Insertion into histone cluster (non-unique read). Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8894 Species: Caenorhabditis elegans Genotype: oxTi944 II; unc-119(ed3) III. Description: oxTi944 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:-17.95). Insertion into C23H3.5. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8895 Species: Caenorhabditis elegans Genotype: oxTi945 unc-119(ed3) III. Description: oxTi945 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] III. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (III:-15.41). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8896 Species: Caenorhabditis elegans Genotype: oxTi946 I; unc-119(ed3) III. Description: oxTi946 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:2.69). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8897 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi947 V. Description: oxTi947 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:-20.00). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8898 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi949 IV. Description: oxTi949 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] IV. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (IV:3.22). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8899 Species: Caenorhabditis elegans Genotype: oxTi951 I; unc-119(ed3) III. Description: oxTi951 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:-0.73). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8900 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi953 X. Description: oxTi953 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] X. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (X:-2.45). Insertion into abts-4. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8901 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi954 X. Description: oxTi954 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] X. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (X:-7.13). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8902 Species: Caenorhabditis elegans Genotype: oxTi956 unc-119(ed3) III. Description: oxTi956 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] III. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (III:0.03). Insertion into trxr-2. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8903 Species: Caenorhabditis elegans Genotype: oxTi957 II; unc-119(ed3) III. Description: oxTi957 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:3.08). Insertion into mnk-1. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8904 Species: Caenorhabditis elegans Genotype: oxTi959 I; unc-119(ed3) III. Description: oxTi959 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:3.91). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8905 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi961 V. Description: oxTi961 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:13.04). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8906 Species: Caenorhabditis elegans Genotype: oxTi962 unc-119(ed3) III. Description: oxTi962 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] III. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (III:-0.35). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8907 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi963 V. Description: oxTi963 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:12.90). Insertion into srbc-27. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8908 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi965 V. Description: oxTi965 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:-2.52). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8909 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi966 V. Description: oxTi966 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:18.45). Insertion into emb-4. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8910 Species: Caenorhabditis elegans Genotype: oxTi967 I; unc-119(ed3) III. Description: oxTi967 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:-1.28). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8911 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi968 X. Description: oxTi968 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] X. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (X:-3.89). Insertion into C09B8.3. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8912 Species: Caenorhabditis elegans Genotype: oxTi969 II; unc-119(ed3) III. Description: oxTi969 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:18.17). Insertion into lin-38. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8913 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi970 IV. Description: oxTi970 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] IV. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (IV:4.92). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8914 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; oxTi971 IV. Description: oxTi971 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + Cbr-unc-119(+)] IV. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (IV:11.69). Insertion into Y64G10A.6. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1661 into unc-119(ed3)(11x outcrosss) with Cbr-unc-119(+) selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8915 Species: Caenorhabditis elegans Genotype: oxTi973 V. Description: oxTi973 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + HygroR] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:-19.85). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1660 into N2 with hygromycin B selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8916 Species: Caenorhabditis elegans Genotype: oxTi974 II. Description: oxTi974 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + HygroR] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:-11.63). Insertion into fbxb-105. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1660 into N2 with hygromycin B selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8917 Species: Caenorhabditis elegans Genotype: oxTi975 III. Description: oxTi975 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + HygroR] III. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (III:-3.94). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1660 into N2 with hygromycin B selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8918 Species: Caenorhabditis elegans Genotype: oxTi976 II. Description: oxTi976 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + HygroR] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:0.84). Insertion into D2085.5. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1660 into N2 with hygromycin B selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8919 Species: Caenorhabditis elegans Genotype: oxTi977 I. Description: oxTi977 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + HygroR] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:2.42). Insertion into T22C1.8. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1660 into N2 with hygromycin B selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8920 Species: Caenorhabditis elegans Genotype: oxTi979 I. Description: oxTi979 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + HygroR] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:5.06). Insertion into B0205.1. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1660 into N2 with hygromycin B selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8921 Species: Caenorhabditis elegans Genotype: oxTi980 V. Description: oxTi980 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + HygroR] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:-18.05). Insertion into srbc-41 promoter. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1660 into N2 with hygromycin B selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8922 Species: Caenorhabditis elegans Genotype: oxTi981 I. Description: oxTi981 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + HygroR] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:24.77). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1660 into N2 with hygromycin B selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8923 Species: Caenorhabditis elegans Genotype: oxTi983 I. Description: oxTi983 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + HygroR] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:-14.58). Insertion into src-1. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1660 into N2 with hygromycin B selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8924 Species: Caenorhabditis elegans Genotype: oxTi984 II. Description: oxTi984 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + HygroR] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:-15.12). Insertion into srh-72. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1660 into N2 with hygromycin B selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8925 Species: Caenorhabditis elegans Genotype: oxTi986 X. Description: oxTi986 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + HygroR] X. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (X:-20.67). Insertion into T08D2.2. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1660 into N2 with hygromycin B selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8926 Species: Caenorhabditis elegans Genotype: oxTi987 V. Description: oxTi987 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + HygroR] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:20.20). Insertion into dyf-17. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1660 into N2 with hygromycin B selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8927 Species: Caenorhabditis elegans Genotype: oxTi988 IV. Description: oxTi988 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + HygroR] IV. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (IV:3.23). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1660 into N2 with hygromycin B selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8928 Species: Caenorhabditis elegans Genotype: oxTi989 III. Description: oxTi989 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + HygroR] III. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (III:19.10). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1660 into N2 with hygromycin B selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8930 Species: Caenorhabditis elegans Genotype: oxTi991 V. Description: oxTi991 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + HygroR] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:5.59). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1660 into N2 with hygromycin B selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8931 Species: Caenorhabditis elegans Genotype: oxTi992 II. Description: oxTi992 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + HygroR] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:-15.96). Insertion into K10B4.1. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1660 into N2 with hygromycin B selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8932 Species: Caenorhabditis elegans Genotype: oxTi993 I. Description: oxTi993 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + PuroR] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:25.92). Insertion into Y105E8B.7. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with puromycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8933 Species: Caenorhabditis elegans Genotype: oxTi994 IV. Description: oxTi994 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + PuroR] IV. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (IV:2.45). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with puromycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8934 Species: Caenorhabditis elegans Genotype: oxTi995 I. Description: oxTi995 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + PuroR] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:-0.03). Insertion into pqn-44. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with puromycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8935 Species: Caenorhabditis elegans Genotype: oxTi996 IV. Description: oxTi996 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + PuroR] IV. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (IV:6.87). Insertion into mbk-2. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with puromycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8936 Species: Caenorhabditis elegans Genotype: oxTi997 V. Description: oxTi997 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + PuroR] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:2.06). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with puromycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8937 Species: Caenorhabditis elegans Genotype: oxTi998 V. Description: oxTi998 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + PuroR] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:6.23). Insertion into F14D7.14. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with puromycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8938 Species: Caenorhabditis elegans Genotype: oxTi999 V. Description: oxTi999 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + PuroR] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:6.72). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with puromycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8939 Species: Caenorhabditis elegans Genotype: oxTi1000 IV. Description: oxTi1000 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + PuroR] IV. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (IV:15.17). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with puromycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8940 Species: Caenorhabditis elegans Genotype: oxTi1001 III. Description: oxTi1001 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + PuroR] III. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (III:-0.45). Insertion into C06G4.6. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with puromycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8941 Species: Caenorhabditis elegans Genotype: oxTi1002 I. Description: oxTi1002 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + PuroR] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:19.25). Insertion into Y40B1A.3. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with puromycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8942 Species: Caenorhabditis elegans Genotype: oxTi1003 X. Description: oxTi1003 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + PuroR] X. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (X:24.09). Insertion into dhs-30 and T24G12.3. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with puromycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8943 Species: Caenorhabditis elegans Genotype: oxTi1005. Description: oxTi1005 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + PuroR] III. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (III:-14.69). Insertion into srd-69. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with puromycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8944 Species: Caenorhabditis elegans Genotype: oxTi1006 V. Description: oxTi1006 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + PuroR] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:-19.95). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with puromycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8945 Species: Caenorhabditis elegans Genotype: oxTi1007 V. Description: oxTi1007 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + NeoR] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:5.53). Insertion into srd-11. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with neomycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8946 Species: Caenorhabditis elegans Genotype: oxTi1008 IV. Description: oxTi1008 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + NeoR] IV. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (IV:3.75). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with neomycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8947 Species: Caenorhabditis elegans Genotype: oxTi1009 I. Description: oxTi1009 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + NeoR] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:-18.96). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with neomycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8948 Species: Caenorhabditis elegans Genotype: oxTi1010 II. Description: oxTi1010 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + NeoR] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:10.79). Insertion into tbc-17. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with neomycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8949 Species: Caenorhabditis elegans Genotype: oxTi1011 II. Description: oxTi1011 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + NeoR] II. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (II:-1.99). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with neomycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8950 Species: Caenorhabditis elegans Genotype: oxTi1014 IV. Description: oxTi1014 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + NeoR] IV. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (IV:4.62). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with neomycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8951 Species: Caenorhabditis elegans Genotype: oxTi1015 X. Description: oxTi1015 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + NeoR] X. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (X:12.63). Insertion into srd-50. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with neomycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8952 Species: Caenorhabditis elegans Genotype: oxTi1016 I. Description: oxTi1016 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + NeoR] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:-18.09). Insertion into Y95B8A.8. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with neomycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8953 Species: Caenorhabditis elegans Genotype: oxTi1017 IV. Description: oxTi1017 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + NeoR] IV. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (IV:3.20). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with neomycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8954 Species: Caenorhabditis elegans Genotype: oxTi1018 I. Description: oxTi1018 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + NeoR] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:21.64). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with neomycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8955 Species: Caenorhabditis elegans Genotype: oxTi1019 X. Description: oxTi1019 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + NeoR] X. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (X:-7.36). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with neomycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8956 Species: Caenorhabditis elegans Genotype: oxTi1020 V. Description: oxTi1020 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + NeoR] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:-2.60). Insertion into C04F5.2. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with neomycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8957 Species: Caenorhabditis elegans Genotype: oxTi1021 V. Description: oxTi1021 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + NeoR] V. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (V:6.85). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with neomycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8958 Species: Caenorhabditis elegans Genotype: oxTi1022 I. Description: oxTi1022 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + NeoR] I. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (I:22.34). Insertion into Y71A12B.8. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with neomycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8959 Species: Caenorhabditis elegans Genotype: oxTi1023 IV. Description: oxTi1023 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + NeoR] IV. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (IV:4.05). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with neomycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8960 Species: Caenorhabditis elegans Genotype: oxTi1024 III. Description: oxTi1024 [eft-3p::GFP::2xNLS::tbb-2 3'UTR + NeoR] III. Strain is healthy. NOTE: This strain is not necessarily homozygous - please verify before using. Nuclear green fluorescence is broadly expressed (in most cells) and visible under dissection microscope. This strain can be used for mapping or to facilitate genetic crosses. Integration site: (III:-3.80). Intergenic insertion. Please see wormbuilder.org for exact insertion site. miniMos insertion of pCFJ1659 into N2 with neomycin selection. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG8961 Species: Caenorhabditis elegans Genotype: oxSi255 I; oxTi81 him-5(e1490) V. Description: oxSi255 [snt-1p::GFP + Cbr-unc-119(+)] I. Integration into ttTi4348 mosSCI site (I:-5.32). Pan-neuronal GFP expression visible under dissection microscope. oxTi81 [eft-3p::GFP::H2B::tbb-2 3'UTR + unc-18(+)] V. Nuclear, green fluorescence is broadly expressed (in most cells). Integration into chr.V: 1.21. Him. Combined fluorescent balancer strain for LG I and LG IV. Strain contains him-5(e1490) to generate males for crosses. Mutagen: Mos1 transposon Outcrossed: x0 Made by: C. Frokjaer-Jensen Received: 10/22/14 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG9631 Species: Caenorhabditis elegans Genotype: unc-13(s69) I. Description: Aldicarb resistant. This allele is a small exonic deletion that frameshifts exon 21, thus deleting the MUN domain of both long and short isoforms of unc-13. The reference allele e51 is R471-stop, affecting only UNC-13L. Derived by 2x outcross of BC168. Reference: Rose AM & Baillie DL. Genetics. 1980 Nov;96(3):639-48. Mutagen: Formaldehyde Outcrossed: x2 Made by: Qiang Liu Received: 03/15/25 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG9747 Species: C. elegans Genotype: oxSi1106 II; unc-119(ed3) III. Description: oxSi1106 [mex-5p::Cas9(+smu-2 introns)::tbb-2 3'UTR + lox2272] II. Integrated Cas9 transgene inserted into ttTi5605 MosSci site. Reference: Schwartz ML, et al. High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome. bioRxiv 2021.08.03.454883; doi: https://doi.org/10.1101/2021.08.03.454883 Mutagen: MosSci / Mosase Outcrossed: x Made by: Matthew Schwartz Received: 11/07/18 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG9814 Species: C. elegans Genotype: unc-119(ox819) III. Description: Crispr/Cas9 engineered mutation in unc-119. ox819 is an 11 bp deletion causing a frameshift after V110 (UNC-119a) and then appends 29 out-of-frame amino acids before a stop codon. unc-119(ox819) animals are phenotypically identical to unc-119(ed3) animals and can be rescued by expression of the smaller C. briggsae unc-119 gene (Cbr-unc-119). Reference: Schwartz ML, et al. High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome. bioRxiv 2021.08.03.454883; doi: https://doi.org/10.1101/2021.08.03.454883 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Matthew Schwartz Received: 08/11/21 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG9876 Species: C. elegans Genotype: unc-119(ox819 oxTi1126) III. Description: oxTi1126 [mex-5p::Cas9(+smu-2 introns)::tbb-2 3'UTR + hsp-16.41p::Cre::tbb-2 3'UTR + myo-2p::2xNLS::cyOFP::let-858 3'UTR + lox2272] III. Knock-in into previously modified unc-119(ox819) endogenous locus. Cas9 insertion marked with myo-2p::cyOFP (cyan-excitable Orange Fluorescent Protein), a long-Stokes-shift fluorescent protein that is spectrally separable from common green and red fluorophores. The Cas9 transgene was optimized for germline expression by including 4 large PATC-rich introns from smu-2. Lower activity than other Cas9 strains, but useful because Cas9, Cre, and unc-119 are in a single unit. Reference: Schwartz ML, et al. High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome. bioRxiv 2021.08.03.454883; doi: https://doi.org/10.1101/2021.08.03.454883 Mutagen: miniMos, Crispr/Cas9 Outcrossed: x Made by: Matthew Schwartz Received: 08/11/21 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG9881 Species: C. elegans Genotype: unc-119(ox819) F53A2.9(oxTi1127) III. Description: oxTi1127 [mex-5p::Cas9(+smu-2 introns)::tbb-2 3'UTR + hsp-16.41p::Cre::tbb-2 3'UTR + myo-2p::2xNLS::cyOFP::let-858 3'UTR + lox2272] III. Inserted into F53A2.9. High Cas9 activity. Integrated Cas9 transgene linked to unc-119(ox819). Cas9 insertion marked with myo-2p::cyOFP (cyan-excitable Orange Fluorescent Protein), a long-Stokes-shift fluorescent protein that is spectrally separable from common green and red fluorophores. Reference: Schwartz ML, et al. High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome. bioRxiv 2021.08.03.454883; doi: https://doi.org/10.1101/2021.08.03.454883 Mutagen: miniMos, Crispr/Cas9 Outcrossed: x Made by: Matthew Schwartz Received: 08/11/21 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG9882 Species: C. elegans Genotype: F53A2.9(oxTi1127) III. Description: oxTi1127 [mex-5p::Cas9(+smu-2 introns)::tbb-2 3'UTR + hsp-16.41p::Cre::tbb-2 3'UTR + myo-2p::2xNLS::cyOFP::let-858 3'UTR + lox2272] III. Inserted into F53A2.9. High Cas9 activity. Superficially wild-type. Cas9 insertion marked with myo-2p::cyOFP (cyan-excitable Orange Fluorescent Protein), a long-Stokes-shift fluorescent protein that is spectrally separable from common green and red fluorophores. Reference: Schwartz ML, et al. High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome. bioRxiv 2021.08.03.454883; doi: https://doi.org/10.1101/2021.08.03.454883 Mutagen: miniMos, Crispr/Cas9 Outcrossed: x2 Made by: Matthew Schwartz Received: 08/11/21 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG9885 Species: C. elegans Genotype: W01A8.6(oxTi1120) I; unc-119(ox819) III. Description: oxTi1120 [mex-5p::Cas9(+smu-2 introns)::tbb-2 3'UTR + hsp-16.41p::Cre::tbb-2 3'UTR + lox2272] I. Inserted into W01A8.6. The Cas9 transgene was optimized for germline expression by including 4 large PATC-rich introns from smu-2. Reference: Schwartz ML, et al. High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome. bioRxiv 2021.08.03.454883; doi: https://doi.org/10.1101/2021.08.03.454883 Mutagen: miniMos, Crispr/Cas9 Outcrossed: x Made by: Matthew Schwartz Received: 08/11/21 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG9887 Species: C. elegans Genotype: W01A8.6(oxTi1128) I; unc-119(ox819) III. Description: oxTi1128 [mex-5p::Cas9(+smu-2 introns)::tbb-2 3'UTR + hsp-16.41p::Cre::tbb-2 3'UTR + myo-2p::2xNLS::cyOFP::let-858 3'UTR + lox2272]) I. Cas9 insertion marked with myo-2p::cyOFP (cyan-excitable Orange Fluorescent Protein), a long-Stokes-shift fluorescent protein that is spectrally separable from common green and red fluorophores. Inserted into W01A8.6. Reference: Schwartz ML, et al. High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome. bioRxiv 2021.08.03.454883; doi: https://doi.org/10.1101/2021.08.03.454883 Mutagen: miniMos, Crispr/Cas9 Outcrossed: x Made by: Matthew Schwartz Received: 08/11/21 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG9888 Species: C. elegans Genotype: W01A8.6(oxTi1128) I. Description: oxTi1128 [mex-5p::Cas9(+smu-2 introns)::tbb-2 3'UTR + hsp-16.41p::Cre::tbb-2 3'UTR + myo-2p::2xNLS::cyOFP::let-858 3'UTR + lox2272]) I. Outcrossed to remove unc-119 mutation. Superficially wild-type. Cas9 insertion marked with myo-2p::cyOFP (cyan-excitable Orange Fluorescent Protein), a long-Stokes-shift fluorescent protein that is spectrally separable from common green and red fluorophores. Inserted into W01A8.6. Reference: Schwartz ML, et al. High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome. bioRxiv 2021.08.03.454883; doi: https://doi.org/10.1101/2021.08.03.454883 Mutagen: miniMos, Crispr/Cas9 Outcrossed: x2 Made by: Matthew Schwartz Received: 08/11/21 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EG9891 Species: C. elegans Genotype: unc-119(ox819) III; W03F9.11(oxTi1121) V. Description: oxTi1121 [mex-5p::Cas9(+smu-2 introns)::tbb-2 3'UTR + hsp-16.41p::Cre::tbb-2 3'UTR + lox2272]) V. The Cas9 transgene was optimized for germline expression by including 4 large PATC-rich introns from smu-2. Inserted into W03F9.11. Reference: Schwartz ML, et al. High-efficiency CRISPR gene editing in C. elegans using Cas9 integrated into the genome. bioRxiv 2021.08.03.454883; doi: https://doi.org/10.1101/2021.08.03.454883 Mutagen: miniMos, Crispr/Cas9 Outcrossed: x Made by: Matthew Schwartz Received: 08/11/21 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: EGD175 Species: C. elegans Genotype: pie-1(ne4301[pie-1::gfp]) III; mex-5(egx1[F294N & F339N]) IV. Description: pie-1(ne4301) inserted GFP into pie-1 locus tagging endogenous pie-1 with GFP. mex-5(egx1[F294N, F339N]) modifies the endogenous mex-5 locus to disrupt zinc finger motifs. Reference: Han et al, Current Biology 2017. Mutagen: Outcrossed: x0 Made by: Bingjie Han Received: 01/30/18 Dartmouth College, Hanover, NH ----------------------------------------------------------------------------- Strain: EGD199 Species: C. elegans Genotype: mex-5(egx1[F294N, F339N]) IV. Description: mex-5(egx1[F294N, F339N]) modifies the endogenous mex-5 locus to disrupt zinc finger motifs. Reference: Han et al, Current Biology 2017. Mutagen: Outcrossed: x4 Made by: Bingjie Han Received: 01/30/18 Dartmouth College, Hanover, NH ----------------------------------------------------------------------------- Strain: EGD224 Species: C. elegans Genotype: egxSi100 II; unc-119(ed3) III. Description: egxSi100 [mex-5p::GFP::pos-1 + unc-119(+)] II. Single-copy transgene expressing GFP::POS-1. Reference: Han et al, Current Biology 2017. Mutagen: Outcrossed: x0 Made by: Bingjie Han Received: 01/30/18 Dartmouth College, Hanover, NH ----------------------------------------------------------------------------- Strain: EGD226 Species: C. elegans Genotype: egxSi101 II; unc-119(ed3) III. Description: egxSi101 [mex-5p::GFP::pos-1(F121N & F164N) + unc-119(+)] II. Single-copy transgene expressing mutated POS-1 with GFP tag. GFP::POS-1 is uniformly distributed in the one-cell zygote. Reference: Han et al, Current Biology 2017. Mutagen: Outcrossed: x0 Made by: Bingjie Han Received: 01/30/18 Dartmouth College, Hanover, NH ----------------------------------------------------------------------------- Strain: EGD263 Species: C. elegans Genotype: egxSi100 II; unc-119(ed3) III; mex-5(egx1[F294N & F339N]) IV. Description: egxSi100 [mex-5p::GFP::pos-1 + unc-119(+)] II. Single-copy transgene expressing GFP::POS-1. mex-5(egx1[F294N, F339N]) modifies the endogenous mex-5 locus to disrupt zinc finger motifs. Reference: Han et al, Current Biology 2017. Mutagen: Outcrossed: x0 Made by: Bingjie Han Received: 01/30/18 Dartmouth College, Hanover, NH ----------------------------------------------------------------------------- Strain: EGD271 Species: C. elegans Genotype: egxSi109 II; unc-119(ed3) III. Description: egxSi109 [mex-5p::GFP::pos-1(S199A, S210A, S216A, S237A & T242A) + unc-119(+)] II. Single-copy transgene expressing mutated POS-1 with GFP tag. GFP::POS-1 is uniformly distributed in the cytoplasm of the one-cell zygote. Reference: Han et al, Current Biology 2017. Mutagen: Outcrossed: x0 Made by: Bingjie Han Received: 01/30/18 Dartmouth College, Hanover, NH ----------------------------------------------------------------------------- Strain: EGD273 Species: C. elegans Genotype: egxSi110 II; unc-119(ed3) III. Description: egxSi110 [mex-5p::GFP::pos-1(S199D, S210D, S216D, S237D & T242D) + unc-119(+)] II. Single-copy transgene expressing mutated POS-1 with GFP tag. GFP::POS-1 is uniformly distributed in the cytoplasm of the one-cell zygote. Reference: Han et al, Current Biology 2017. Mutagen: Outcrossed: x0 Made by: Bingjie Han Received: 01/30/18 Dartmouth College, Hanover, NH ----------------------------------------------------------------------------- Strain: EGD275 Species: C. elegans Genotype: egxSi116 II; unc-119(ed3) III Description: egxSi116 [mex-5p::GFP::mex-5(F294N, F339N) + unc-119(+)] II. Modified MEX-5::GFP forms a much weaker gradient in the cytoplasm of the one-cell zygote than wild type. Reference: Han et al, Current Biology 2017. Mutagen: Outcrossed: x0 Made by: Bingjie Han Received: 01/30/18 Dartmouth College, Hanover, NH ----------------------------------------------------------------------------- Strain: EGD282 Species: C. elegans Genotype: egxSi100 II; unc-119(ed3) III; mex-5(egx2[T186A]) IV. Description: egxSi100 [mex-5p::GFP::pos-1 + unc-119(+)] II. Single-copy transgene expressing GFP::POS-1 forms a weaker gradient in the cytoplasm of the one-cell zygote than in wild-type. Reference: Han et al, Current Biology 2017. Mutagen: Outcrossed: x0 Made by: Bingjie Han Received: 01/30/18 Dartmouth College, Hanover, NH ----------------------------------------------------------------------------- Strain: EGD329 Species: C.elegans Genotype: egxSi126 I; unc-119(ed3) III Description: egxSi126 [mex-5p::hsp-3(aa1-19)::halotag::HDEL::pie-1 3’UTR + unc-119(+)] I. Superficially wild-type. Stable expression of Halotag in the ER lumen in the germline and embryos. Reference: Fan X, et al. G3 (accepted). Mutagen: Outcrossed: x0 Made by: Xintao Fan Received: 12/20/19 Dartmouth College, Hanover, NH ----------------------------------------------------------------------------- Strain: EGD334 Species: C. elegans Genotype: egxSi100 II; plk-1(egx3[C52V, L115G]); unc-119(ed3) III. Description: egxSi100 [mex-5p::GFP::pos-1 + unc-119(+)] II. Single-copy transgene expressing GFP::POS-1. Reference: Han et al, Current Biology 2017. Mutagen: Outcrossed: x0 Made by: Bingjie Han Received: 01/30/18 Dartmouth College, Hanover, NH ----------------------------------------------------------------------------- Strain: EGD412 Species: C.elegans Genotype: egxSi136 II; unc-119(ed3) III Description: egxSi136 [mex-5p::tomm-20::halotag::pie-1 3’UTR + unc-119(+)] II. Superficially wild-type. Stable expression of TOMM-20 tagged with Halo on the outer membranes of mitochondria in the germline and embryos. Reference: Fan X, et al. G3 (accepted). Mutagen: Outcrossed: x0 Made by: Xintao Fan Received: 12/20/19 Dartmouth College, Hanover, NH ----------------------------------------------------------------------------- Strain: EGD549 Species: C.elegans Genotype: egxSi144 II; unc-119(ed3) III. Description: egxSi144 [mex-5p::cox-4::halotag::pie-1 3’UTR + unc-119 (+)] II. Superficially wild-type. Stable expression of COX-4 tagged with Halo in the mitochondrial matrix in the germline and embryos. Reference: Fan X, et al. G3 (accepted). Mutagen: Outcrossed: x0 Made by: Xintao Fan Received: 12/20/19 Dartmouth College, Hanover, NH ----------------------------------------------------------------------------- Strain: EGD565 Species: C.elegans Genotype: egxSi145 II; unc-119(ed3) III. Description: egxSi145 [mex-5p::hsp-3(aa1-19)::halotag::HDEL::pie-1 3’UTR + unc-119(+)] II. Superficially wild-type. Stable expression of Halotag in the ER lumen in the germline and embryos. Reference: Fan X, et al. G3 (accepted). Mutagen: Outcrossed: x0 Made by: Xintao Fan Received: 12/20/19 Dartmouth College, Hanover, NH ----------------------------------------------------------------------------- Strain: EGD615 Species: C.elegans Genotype: cox-4(zu476[cox-4::eGFP::3XFLAG]) I; egxSi136 II; unc-119(ed3) III. Description: egxSi136 [mex-5p::tomm-20::halotag::pie-1 3’UTR + unc-119(+)] II. GFP and 3xFLAG tags inserted into endogenous cox-4 locus to create a C-terminal translational GFP fusion. Outer membranes are stably labeled with the TOMM-20::Halotag transgene, and the mitochondria matrix are labeled with COX-4::GFP. Reference: Fan X, et al. G3 (accepted). Mutagen: Outcrossed: x0 Made by: Stephanie Miller Received: 12/20/19 Dartmouth College, Hanover, NH ----------------------------------------------------------------------------- Strain: EGD623 Species: C. elegans Genotype: egxSi152 II; unc-119(ed3) III. Description: egxSi152 [mex-5p: tomm-20::gfp::pie-1 3’UTR + unc-119(+)] II. Superficially wild-type. Stable expression of TOMM-20 tagged with GFP on the outer membranes of mitochondria in the germline and embryos. Reference: Fan X, et al. G3 (accepted). Mutagen: Outcrossed: x0 Made by: Xintao Fan Received: 12/20/19 Dartmouth College, Hanover, NH ----------------------------------------------------------------------------- Strain: EGD629 Species: C. elegans Genotype: egxSi155 II; unc-119(ed3) III. Description: egxSi155 [mex-5p::tomm-20::mKate2::pie-1 3’UTR + unc-119(+)] II. Superficially wild-type. Stable expression of TOMM-20 tagged with mKate2 on the outer membranes of mitochondria in the germline and embryos. Reference: Fan X, et al. G3 (accepted). Mutagen: Outcrossed: x0 Made by: Stephanie Miller Received: 12/20/19 Dartmouth College, Hanover, NH ----------------------------------------------------------------------------- Strain: EGD631 Species: C.elegans Genotype: egxSi157 II; unc-119(ed3) III. Description: egxSi157 [mex-5p::tomm-20::Dendra2::pie-1 3’UTR + unc-119(+)] II. Superficially wild-type. Stable expression of TOMM-20 tagged with photoconvertible Dendra2 on the outer membranes of mitochondria in the germline and embryos. Reference: Fan X, et al. G3 (accepted). Mutagen: Outcrossed: x0 Made by: Stephanie Miller Received: 12/20/19 Dartmouth College, Hanover, NH ----------------------------------------------------------------------------- Strain: EH135 Species: Caenorhabditis elegans Genotype: unc-44(e362) bli-6(sc16) IV. Description: Blistered. Unc. Mutagen: Outcrossed: x Made by: T. Stiernagle Received: 08/08/94 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: EH250 Species: Caenorhabditis elegans Genotype: sma-3(e491) unc-32(e189) III. Description: Mutagen: Outcrossed: x Made by: T. Stiernagle Received: 08/22/94 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: EH487 Species: Caenorhabditis elegans Genotype: inx-3(lw68) X; lwEx27. Description: lwEx27 [inx-3::GFP]. inx-3(lw68) embryos display a wide range of developmental defects. All L1s that manage to hatch have a Pun (pharynx unattached) phenotype. inx-3::GFP transgene rescues lw68 to wild-type. Pick L2s or later to maintain. Reference: Starich, et al., 2003. Dev. Biol. 256:403. Mutagen: Outcrossed: x3 Made by: Todd Starich Received: 03/28/17 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: EJ1158 Species: Caenorhabditis elegans Genotype: gon-2(q388) I. Description: NOTE: Supplement media to 50 mM Mg2+ and grow at 15C for maximum fertility. Temperature-sensitive failure of gonad precursor divisions. Penetrance of Gon phenotype is high at 23.5C. At 25 degrees you can expect reduced brood sizes and some embryonic lethality. [Note: temperature sensitive period for gon-2(q388) begins prior to fertilization.] Reference: Sun AY & Lambie EJ. Genetics. 1997 Nov;147(3):1077-89. Mutagen: EMS Outcrossed: x4 Made by: Eric Lambie Received: 03/13/17 University College London ----------------------------------------------------------------------------- Strain: EJ1167 Species: Caenorhabditis elegans Genotype: gem-1(bc364) X. Description: bc364 deletes 1,109 bp between AACATCTTGAATAACCATTCGGGAAGT and AAGTCATTCATTGCAGAGCTTACATTTAGTA. References: Kemp BJ, et al. Genetics. 2009 Feb;181(2):581-91. Mutagen: EMS Outcrossed: x4 Made by: Eric Lambie Received: 03/13/17 University College London ----------------------------------------------------------------------------- Strain: EJ1171 Species: Caenorhabditis elegans Genotype: gon-2(q388) I; gem-1(bc364) X. Description: NOTE: Supplement media to 50 mM Mg2+ and grow at 15C for maximum fertility. The stock will propagate on non-supplemented media at 20 degrees, but this will potentially select for intragenic revertants of gon-2(q388). Temperature-sensitive failure of gonad precursor divisions. Penetrance of Gon phenotype is very high at 23.5C. At 25 degrees you can expect reduced brood sizes and some embryonic lethality. [Note: temperature sensitive period for gon-2(q388) begins prior to fertilization.] bc364 deletes 1,109 bp between AACATCTTGAATAACCATTCGGGAAGT and AAGTCATTCATTGCAGAGCTTACATTTAGTA. References: Kemp BJ, et al. Genetics. 2009 Feb;181(2):581-91. Sun AY & Lambie EJ. Genetics. 1997 Nov;147(3):1077-89. Mutagen: EMS Outcrossed: x4 Made by: Eric Lambie Received: 03/13/17 University College London ----------------------------------------------------------------------------- Strain: EJ238 Species: Caenorhabditis elegans Genotype: mek-2(q425) unc-11(e47) I; sDp2 (I;f). Description: Animals with the duplication are WT. Animals which have lost the duplication are Unc, Sterile and Vul at all temperatures. Mutagen: EMS Outcrossed: x>6 Made by: Eric Lambie Received: 12/08/95 University College London ----------------------------------------------------------------------------- Strain: EJ255 Species: Caenorhabditis elegans Genotype: mek-2(q484) I; sDp2 (I;f). Description: Animals with the duplication are WT. Animals which have lost the duplication are Sterile and Vul. At 15C rare animals will have a protruding vulva. At 20C and 25C animals with protruding vulva are more common, about 10%. Mutagen: UV Outcrossed: x>4 Made by: Eric Lambie Received: 12/08/95 University College London ----------------------------------------------------------------------------- Strain: EJ26 Species: Caenorhabditis elegans Genotype: gon-2(q362) I. Description: Starvation sensitive gonadogenesis defect. Mutagen: EMS Outcrossed: x>4 Made by: Eric Lambie Received: 09/04/01 University College London ----------------------------------------------------------------------------- Strain: EJ275 Species: Caenorhabditis elegans Genotype: unc-29(e1072)/dxDf1 I. Description: Heterozygotes are WT and segregate WT, Uncs and dead eggs. Mutagen: UV Outcrossed: x>1 Made by: Received: 09/28/98 University College London ----------------------------------------------------------------------------- Strain: EJ374 Species: Caenorhabditis elegans Genotype: gon-2(dx22) fer-1(hc1) unc-29(e1072) I. Description: Unc. Temperature senstive for both gon-2 and fer-1. Maintain at 15C. Will also grow at 20C. Received new stock 10/12/00 from EJ. Mutagen: EMS Outcrossed: x>1 Made by: Received: 09/15/98 University College London ----------------------------------------------------------------------------- Strain: EJ420 Species: Caenorhabditis elegans Genotype: gon-2(dx23) fer-1(hc1) I. Description: Temperature sensitive for both gon-2 and fer-1. Maintain at 15C. Will also grow at 20C. Mutagen: EMS Outcrossed: x>1 Made by: Received: 09/15/98 University College London ----------------------------------------------------------------------------- Strain: EJ521 Species: Caenorhabditis elegans Genotype: lin-45(dx19) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Unc, non-Unc Steriles, larval lethals and dead eggs. dx19 is a strong lin-45 raf allele: dx19 homozygotes from dx19/+ heterozygous parents are 13% larval lethal and among the adults, 100% are Sterile and Vul. Mutagen: UV Outcrossed: x4 Made by: Eric Lambie Received: 06/14/02 Washington University School of Medicine, St. Louis, MO ----------------------------------------------------------------------------- Strain: EJ808 Species: Caenorhabditis elegans Genotype: gem-4(dx77) IV. Description: No apparent phenotype. Suppressor of gon-2(q388). Mutagen: Outcrossed: x5 Made by: Eric Lambie Received: 04/16/04 University College London ----------------------------------------------------------------------------- Strain: EJ810 Species: Caenorhabditis elegans Genotype: F25H2.5(ok314)/hT2 [dpy-18(h662)] I; +/hT2 [bli-4(e937)] III; him-8(e1489) IV. Description: Him. Heterozygotes are WT and segregate WT, Sterile Evul and Dpys. Mutagen: UV/TMP Outcrossed: x1 Made by: G. Moulder Received: 05/20/15 University College London ----------------------------------------------------------------------------- Strain: EK123 Species: Caenorhabditis elegans Genotype: cmEx6. Description: cmEx6 [(pBR126) mbk-2p::GFP + rol-6(su1006)]. Maintain by picking Rollers. Mutagen: Outcrossed: x Made by: Received: 12/22/03 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: EK224 Species: Caenorhabditis elegans Genotype: cmIs6 I; unc-4(e120) II. Description: cmIs6 [(pBR104) mbk-1::GFP + pNC4.21]. Mutagen: Outcrossed: x6 Made by: Received: 12/22/03 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: EK228 Species: Caenorhabditis elegans Genotype: mbk-1(pk1389) X. Description: Homozygous viable. Weak olfaction defects at dilute concentrations of chemoattractants. Probable null allele. Mutagen: Outcrossed: x6 Made by: C Moorman/B Raich Received: 04/01/02 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: EK273 Species: Caenorhabditis elegans Genotype: hpk-1(pk1393) X. Description: Homozygous viable. Males do not mate (or mate poorly). Probable null allele. Mutagen: Outcrossed: x6 Made by: C Moorman/B Raich Received: 04/01/02 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: EKM11 Species: Caenorhabditis elegans Genotype: htz-1(tm2469) IV/nT1 [qIs51] (IV;V). Description: Maintain under normal condition. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP tm2469 homozygotes (Sterile). tm2469 m+z- are sterile adults. Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Petty EL, et al. PLoS Genet. 2009 Oct;5(10):e1000699. doi: 10.1371/journal.pgen.1000699. Csankovszki G, et al. (2009) Curr Biol 19(1):9-19. [NOTE: new stock received at CGC 05/26/2021. Original stock received in 2010 had broken down and was no longer segregating as expected.] Mutagen: TMP/UV Outcrossed: x6 Made by: G. Csankovszki Received: 05/26/21 University of Michigan, Ann Arbor, MI ----------------------------------------------------------------------------- Strain: EKM4 Species: Caenorhabditis elegans Genotype: capg-1(tm1514) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Homozygous sterile deletion balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP tm1514 homozygotes (sterile, tm1514 m+z- are larval lethal). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Csankovszki G, et al. (2009) Curr Biol 19(1):9-19. Mutagen: TMP/UV Outcrossed: x8 Made by: G. Csankovszki Received: 06/04/10 University of Michigan, Ann Arbor, MI ----------------------------------------------------------------------------- Strain: EKM42 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; cldIs5. Description: cldIs5 [pie-1p::capg-1::GFP + unc-119(+)]. GFP tagged CAPG-1 driven by the pie-1 promoter; expression is detected in the germline and in embryos. References: Collette K, et al. J Cell Sci. 2011 Nov 1;124(Pt 21):3684-94. Bembenek JN, et al. Curr Biol. 2013 Jun 3;23(11):937-46. Mutagen: Outcrossed: x10 Made by: Joshua Bembenek Received: 03/27/14 University of Michigan, Ann Arbor, MI ----------------------------------------------------------------------------- Strain: EL129 Species: Caenorhabditis elegans Genotype: ego-3(om40) unc-76(e911) V/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate additional hets, Unc-76 om40 homozygotes and dead eggs. om40 animals have multiple germline defects. Mutagen: EMS Outcrossed: x Made by: Received: 02/18/97 Syracuse University, Syracuse, NY ----------------------------------------------------------------------------- Strain: EL186 Species: Caenorhabditis elegans Genotype: dpy-19(e1259) ego-4(om30)/eT1 III; +/eT1 V. Description: Heterozygotes are WT and segregate WT, Dpy (ts) Mel, Unc-36 and dead eggs. dpy-19 ego-4 progeny die as embryos. Mutagen: EMS Outcrossed: x Made by: Received: 02/11/97 Syracuse University, Syracuse, NY ----------------------------------------------------------------------------- Strain: EL187 Species: Caenorhabditis elegans Genotype: dpy-19(e1259) ego-5(om31)/eT1 III; +/eT1 V. Description: Heterozygotes are WT and segregate WT, Dpy (ts) Mel, Unc-36 and dead eggs. Mutagen: EMS Outcrossed: x Made by: Received: 02/18/97 Syracuse University, Syracuse, NY ----------------------------------------------------------------------------- Strain: EL301 Species: Caenorhabditis elegans Genotype: lag-1(om13) IV. Description: Temperature sensitive; best grown at 15C. Lab and embryonic Mel phenotypes. Mutagen: EMS Outcrossed: x Made by: Received: 02/11/97 Syracuse University, Syracuse, NY ----------------------------------------------------------------------------- Strain: EL302 Species: Caenorhabditis elegans Genotype: ego-1(om71) unc-29(e193)/hT2 [dpy-18(h662)] I; +/hT2 [bli-4(e937)] III. Description: Heterozygotes are WT and segregate WT, Sterile Uncs and Dpys. bli-4 is suppressed by dpy-18 in hT2 homozygotes-only see a very few DpyBli. Mutagen: EMS Outcrossed: x Made by: Received: 02/11/97 Syracuse University, Syracuse, NY ----------------------------------------------------------------------------- Strain: EL322 Species: Caenorhabditis elegans Genotype: ego-2(om33) fer-6(om117) I; him-5(e1467) V. Description: om33 chromosome contains a linked fer-6 allele and a linked Mel. Mel probably not associated with om33, but they have not been separated. Best grown at 15C. Mutagen: EMS Outcrossed: x Made by: Received: 02/18/97 Syracuse University, Syracuse, NY ----------------------------------------------------------------------------- Strain: EL329 Species: Caenorhabditis elegans Genotype: ego-1(om58)/hT2 [dpy-18(h662)] I; +/hT2 [bli-4(e937)] III. Description: Heterozgyotes are WT and segregate WT, Steriles and Dpys. The Steriles produce small, abnormal oocytes; some dead embryos are produced in late adulthood. bli-4 is suppressed by dpy-1 in hT2 homozygotes-only see a very few DpyBli. om58 previously called ego-6(om58). Mutagen: EMS Outcrossed: x Made by: Received: 02/11/97 Syracuse University, Syracuse, NY ----------------------------------------------------------------------------- Strain: EL391 Species: Caenorhabditis elegans Genotype: ego-1(om84) unc-29(e193)/hT2 [dpy-18(h662)] I; +/hT2 [bli-4(e937)] III. Description: Heterozygotes are WT and segregate WT, mild Unc that are Sterile, and Dpys. bli-4 is suppressed by dpy-18 in hT2 homozygotes-only see a very few DpyBli. Mutagen: UV Outcrossed: x>2 Made by: Spoerke/Maine Received: 09/15/00 Syracuse University, Syracuse, NY ----------------------------------------------------------------------------- Strain: EL476 Species: Caenorhabditis elegans Genotype: rrf-2(ok210) I. Description: M01G12.12. External left primer: GAGTGGTGGGCAATTGAGTT. External right primer: CCACCCAAGATCTGGTCAGT. Internal left primer: TGTCAACTTGAGGATCGACG. Internal right primer: ATTCCTGCAATTGGTCAAGG. Internal WT amplicon: 2509 bp. Deletion size: 979 bp. Deletion left flank: ATTTTCAACGAAAGTTTTTCGGTTATTTCA. Deletion right flank: AGAGGAAAAAATACGGACAAGAAGAATAAT. Mutagen: UV/TMP Outcrossed: x0 Made by: G Moulder Received: 01/14/02 Syracuse University, Syracuse, NY ----------------------------------------------------------------------------- Strain: EL477 Species: Caenorhabditis elegans Genotype: iffb-1(bc367)/sC1 [dpy-1(s2170)] III. Description: Heterozygotes are WT and segregate WT, Dpy sC1 homozygotes, and bc367 homozygotes which arrest as late L1/early L2 larvae (survive for a few days, then die). Mutagen: EMS Outcrossed: x5 Made by: Xiang Yu Received: 06/04/07 Syracuse University, Syracuse, NY ----------------------------------------------------------------------------- Strain: EL69 Species: Caenorhabditis elegans Genotype: unc-32(e189) glp-1(q231) III; sog-3(q294) IV. Description: Unc. Fertile with viable progeny at or below 20C. Glp-1 sterile at higher temperatures. No obvious visible phenotype associated with sog-3. Mutagen: EMS Outcrossed: x2 Made by: Eleanor Maine Received: 06/23/94 Syracuse University, Syracuse, NY ----------------------------------------------------------------------------- Strain: EM101 Species: Caenorhabditis elegans Genotype: lin-41(bx37) I; him-5(e1490) V. Description: Maintain under normal conditions. Males have long tail tips. Mutagen: Outcrossed: x Made by: Karin Kiontke Received: 07/03/12 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM105 Species: Caenorhabditis elegans Genotype: mab-21(bx41) III; him-5(e1490) V. Description: Transformation of ray 6 to a thin ray which is anteriorly displaced and fuses with ray 4 (95%). A 10th ray is found in about 50% of the sides scored. Body is slightly shorter. Mutagen: EMS Outcrossed: x>5 Made by: Scott Baird Received: 04/14/93 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM106 Species: Caenorhabditis elegans Genotype: lin-41(bx42) I; him-5(e1490) V. Description: Maintain under normal conditions. Males have long tail tips. Mutagen: Outcrossed: x Made by: Karin Kiontke Received: 07/03/12 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM111 Species: Caenorhabditis elegans Genotype: him-5(e1490) V; mab-19(bx38) X. Description: Male phenotype: loss of rays 7-9; incomplete penetrance/expressivity (80%); T lineage defect. Hermaphrodite phenotype: lowered brood size (100 progeny/hermaphrodite). Hypomorphic allele: uDf1/mab-19(bx38) results in embryonic arrest during morphogenesis. Mutagen: EMA Outcrossed: x10 Made by: Scott Baird Received: 04/14/93 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM113 Species: Caenorhabditis elegans Genotype: dpy-10(e128) ram-2(bx32) II; him-5(e1490) V. Description: Dpy. Abnormal rays. Mutagen: Outcrossed: x2 Made by: Baird S Received: 07/30/90 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM116 Species: Caenorhabditis elegans Genotype: mab-25(bx27) I; him-5(e1490) V. Description: Temperature sensitive. Missing Ray. Swollen tail and reduced fan. Temperature sensitive lethal at all stages. Wrinkled spicule. Mutagen: EMS Outcrossed: x Made by: Marie Sutherlin Received: 04/14/93 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM122 Species: Caenorhabditis elegans Genotype: stDp2 (X;II)/+ II; him-5(e1490) V; unc-18(e81) dpy-6(e14) X. Description: Strain throws WT, DpyUncs and males (and some Lon-don't know where these come from??). Maintain by picking WT. non-Unc non-Dpy males mate at low frequency and will transmit stDp2. Mutagen: Outcrossed: x Made by: R. Waterston Received: 06/19/89 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM128 Species: Caenorhabditis elegans Genotype: mab-21(bx53) III; him-5(e1490) V. Description: Transformation of ray 6 to a thin ray which is anteriorly displaced and fuses with ray 4 (95%). A 10th ray is found in about 50% of the sides scored. Slightly shorter than WT. mab-21(bx53)/yDf10 is embryonically lethal with embryo arrest at 2 fold stage. Mutagen: EMS Outcrossed: x5 Made by: Scott Baird Received: 12/14/92 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM131 Species: Caenorhabditis elegans Genotype: ram-2(bx39) II; him-5(e1490) V. Description: Lumpy rays. Mutagen: EMS Outcrossed: x5 Made by: Baird S Received: 07/30/90 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM139 Species: Caenorhabditis elegans Genotype: ram-1(bx34) I; him-5(e1490) V. Description: Lumpy rays. Mutagen: EMS Outcrossed: x3 Made by: Baird S Received: 07/30/90 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM140 Species: Caenorhabditis elegans Genotype: dpy-5(e61) ram-1(bx34) I; him-5(e1490) V. Description: Dpy. Rays abnormal. Mutagen: Outcrossed: x2 Made by: Baird S Received: 07/30/90 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM141 Species: Caenorhabditis elegans Genotype: unc-17(e113) col-34(bx25) IV; him-5(e1490) V. Description: Unc. Abnormal rays. bx25 previously called ram-4. Mutagen: Outcrossed: x3 Made by: Baird S Received: 07/30/90 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM146 Species: Caenorhabditis elegans Genotype: dpy-10(e128) ram-2(bx39) II; him-5(e1490) V. Description: Dpy. Abnormal rays. Mutagen: Outcrossed: x2 Made by: Baird S Received: 07/30/90 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM172 Species: Caenorhabditis elegans Genotype: unc-4(e120) II; him-5(e1490) V; lin-32(bx46) X. Description: Missing Rays. Unc. Mutagen: EMS Outcrossed: x4 Made by: Duc Nguyen Received: 04/14/93 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM185 Species: Caenorhabditis elegans Genotype: tra-1(e1099) III; ram-5(bx30) X; eDp6 (III;f). Description: Animals with the duplication are WT. XX Animals which have lost the duplication are males (low fertility-gonad morphology variable). Rays abnormal. See also WBPaper0000498. Mutagen: Outcrossed: x1 Made by: Baird S Received: 07/30/90 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM195 Species: C.elegans Genotype: lep-2(bx73) IV; him-5(e1490) V. Description: lep-2/Y55F3AM.6 reference allele. Him. Reference: Herrera RA, et al. Development. 2016 Mar 1;143(5):799-809. doi: 10.1242/dev.132738. PMID: 26811380. Mutagen: Outcrossed: x4 Made by: Heike Schirmacher Received: 08/10/23 New York University, New York ----------------------------------------------------------------------------- Strain: EM207 Species: Caenorhabditis elegans Genotype: tbx-2(bx59) III; him-5(e1490) V. Description: Conditional lethal: inviable when grown at 25C. Wild type at 16C. Viable when grown at 20C; partial ray loss at 20C. Shifting males during ray assembly to non-permissive temperature results in ray loss. No lineage defect. Mutagen: EMS Outcrossed: x3 Made by: Marie Sutherlin Received: 04/14/93 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM253 Species: Caenorhabditis elegans Genotype: mab-20(bx61) I; him-5(e1490) V. Description: Ray 3 and 4 fusion >60% at non-permissive temp. Ray 1 and 2 fusion about 10% at non-permissive temp. ts period is around L3-L4. Mutagen: EMS Outcrossed: x>3 Made by: Iman Kassem Received: 04/14/93 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM305 Species: Caenorhabditis elegans Genotype: efn-4(bx80) IV; him-5(e1490) V. Description: Extensive ray fusion involving all 9 rays. Larva have Vab phenotype with decreasing expressivity in adult. Hermaphrodites have swollen tail and anus. bx80 pka mab-26(bx80). Mutagen: Diepoxy butane Outcrossed: x>5 Made by: King Chow Received: 04/14/93 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM331 Species: Caenorhabditis elegans Genotype: him-5(e1490) V; mab-19(bx83) X. Description: Male phenotype: loss of rays 7-9; incomplete penetrance/expressivity (80%); T lineage defect. Hermaphrodite phenotype: lowered brood size (100 progeny/hermaphrodite). Hypomorphic allele: uDf1/mab-19(bx83) results in embryonic arrest during morphogenesis. Mutagen: Diepoxy butane Outcrossed: x>5 Made by: King Chow Received: 04/14/93 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM347 Species: Caenorhabditis elegans Genotype: tlp-1(bx85) IV; him-5(e1490) V. Description: Although hermaphrodites appear WT in other ways, there are some problems with T cell lineages (affecting the phasmids) and tail cell fusions. Variably Dyf. Male tail tip morphogenesis is also defective, resulting in blobby, "leptoderan" tails. Males are infertile due to an inability to properly copulate. tlp-1 encodes a nuclear protein with a single C2H2-type zinc finger domain and an N-terminal "SPLALLA" domain, similar to that of Sp1 transcription factors of vertebrates. The bx85 mutation involves a truncation of TLP-1 due to a frameshift caused by a 5-bp deletion. Mutagen: Diethoxybutane Outcrossed: x Made by: Y Zhang Received: 10/21/02 New York University, New York ----------------------------------------------------------------------------- Strain: EM434 Species: Pellioditis pelhamensis Genotype: Pellioditis pelhamensis wild isolate. Description: WT strain, Pellioditis pelhamensis. See WBG 12(5)14. Male/Female strain. Previously and incorrectly called Rhabditis maupasi by the CGC. Also previously called DF5039. Mutagen: Outcrossed: x Made by: Baird S Received: 04/23/90 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM435 Species: Oscheius myriophilus Genotype: Oscheius myriophilus wild isolate. Description: WT strain. Isolated by D. Fitch in June 1990 from soil in Scott Emmons' compost heap in the Fort Greene section of Brooklyn, NY. A second strain, DF5038, was isolated from the same location one year later from the head and ventral segments of a male pill bug (Armadillidium vulgare). Hermaphroditic. Males are easily isolated by heat shocking L4 or early adult hermaphrodites at 30C for 6-12 hrs. Grows well at 6-25C on OP50. Dauer larvae accumulate under starved or overcrowded conditions. Freezes easily using C. elegans protocols with 90% viability. Previously called Rhabditis sp. See also WBPaper00003418. Formerly known as Oscheius myriophila. Mutagen: Outcrossed: x Made by: David Fitch Received: 08/29/94 New York University, New York ----------------------------------------------------------------------------- Strain: EM437 Species: Pelodera teres Genotype: Pelodera teres wild isolate. Description: WT strain, Pelodera teres. See WBG 12(5) 14. Male/Female strain. Previously called DF5016. Mutagen: Outcrossed: x Made by: Baird S Received: 09/05/90 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM464 Species: Caenorhabditis remanei ssp. vulgaris Genotype: C. remanei ssp. vulgaris Description: Male-female strain. Reference WBG 11(4):89. See also WBPaper00001874. May crawl off the plates. Previously called C. vulgaris BK and C. vulgariensis BK. Walter Sudhaus has tentatively described this strain as C. remanei ssp. vulgaris; this description is not official and is contigent upon its being published. See WBPaper00002633 and WBPaper00003993. Mutagen: Outcrossed: x Made by: Baird S Received: 12/26/90 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM489 Species: Caenorhabditis elegans Genotype: pal-1(e2091) III; him-5(e1490) V; dpy-22(bx92) X. Description: V6 produces rays instead of alae in pal-1; sop01 mutants. No obvious phenotype in a pal-1(+) background. Previously called sop-1(bx92). Mutagen: EMS Outcrossed: x2 Made by: Hong Zhang Received: 10/25/00 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM490 Species: Caenorhabditis elegans Genotype: pal-1(e2091) III; him-5(e1490) V; dpy-22(bx93) X. Description: V6 produces rays instead of alae in pal-1; sop01 mutants. No obvious phenotype in a pal-1(+) background. Previously called sop-1(bx93). Mutagen: EMS Outcrossed: x2 Made by: Hong Zhang Received: 10/25/00 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM493 Species: Caenorhabditis elegans Genotype: sop-3(bx96) I; pal-1(e2091) III; him-5(e1490) V. Description: V6 produces rays instead of alae in sop-3; pal-1 mutants. Mutagen: EMS Outcrossed: x2 Made by: Hong Zhang Received: 10/25/00 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM496 Species: Caenorhabditis elegans Genotype: hlh-2(bx108) I; him-5(e1490) V; lin-32(e1926) X. Description: Males are rayless. hlh-2(bx108) strongly and semi-dominantly enhances the ray-loss phenotype of lin-32(e1926). Mutagen: EMS Outcrossed: x>8 Made by: Doug Portman Received: 09/04/01 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM507 Species: Caenorhabditis elegans Genotype: pal-1(e2091) III; him-5(e1490) V; dpy-22(bx103) X. Description: V6 produces rays instead of alae in pal-1; sop-1 mutants. No obvious phenotype in a pal-1(+) background. Previously called sop-1(bx103). Mutagen: EMS Outcrossed: x2 Made by: Hong Zhang Received: 10/25/00 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM511 Species: Caenorhabditis elegans Genotype: pal-1(e2091) III; him-5(e1490) V; dpy-22(bx107) X. Description: V6 produces rays instead of alae in pal-1; sop01 mutants. No obvious phenotype in a pal-1(+) background. Previously called sop-1(bx107). Mutagen: EMS Outcrossed: x2 Made by: Hong Zhang Received: 10/25/00 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM598 Species: Caenorhabditis elegans Genotype: hlh-2(bx115) unc-13(e51) I; him-5(e1490) V. Description: Paralyzed Unc. Throws males. hlh-2(bx115) has no phenotype in this background. Mutagen: EMS Outcrossed: x4 Made by: Doug Portman Received: 09/04/01 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM599 Species: Caenorhabditis elegans Genotype: him-5(e1490) V; lin-15B&lin-15A(n765) X; bxIs13. Description: bxIs13 [egl-5::GFP + lin-15(+)]. Him. egl-5::GFP reporter made from EM#286 (GFP inserted within the last few amino acids of the C-terminus) by integrating bxEx30 in EM588. High nuclear expression of transgene in males (good in seam cells, none in rectal epithelium), weak expression in hermaphrodites. Expression in 2-4 head neurons in males and hermaphrodites. egl-5 gene on reporter does not rescue egl-5(-). Mutagen: Outcrossed: x2 Made by: H. Ferreira Received: 05/17/02 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM62 Species: Caenorhabditis elegans Genotype: mab-17(e2167) unc-15(e73) I; him-5(e1490) V. Description: Unc. Round bulging adult male tale. Very reduced fan. Mating efficiency is zero. Mutagen: EMS Outcrossed: x3 Made by: Scott Emmons Received: 04/14/93 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM66 Species: Caenorhabditis elegans Genotype: him-5(e1490) V; vab-3(bx23) X. Description: Transformation of ray 6 to a thin ray which is anteriorly displaced and fuses with ray 4 (99%). Body is slightly shorter. See also WBPaper00002235. Mutagen: EMS Outcrossed: x3 Made by: Scott Baird Received: 04/14/93 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM67 Species: Caenorhabditis elegans Genotype: mab-20(bx24) I; him-5(e1490) V. Description: Extensive ray fusion. Posterior portion of body swollen at larval stages. Mutagen: EMS Outcrossed: x>3 Made by: Scott Baird Received: 04/14/93 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM68 Species: Caenorhabditis elegans Genotype: col-34(bx25) IV; him-5(e1490) V. Description: Lumpy rays. Temperature sensitive. bx25 previously called ram-4. Mutagen: EMS Outcrossed: x4 Made by: Baird S Received: 07/30/90 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM723 Species: Caenorhabditis elegans Genotype: sop-2(bx91) II; him-5(e1490) V. Description: Temperature sensitive. Early larval arrest at 25C. At 20C, Lon, PVul, Muv, Mog, ectopic Hox gene expression. Mutagen: EMS Outcrossed: x>1 Made by: H. Zhang/S. Emmons Received: 04/01/05 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM81 Species: Caenorhabditis elegans Genotype: him-5(e1490) V; ram-5(bx30) X. Description: Lumpy rays. Mutagen: EMS Outcrossed: x3 Made by: Baird S Received: 07/30/90 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM886 Species: Caenorhabditis elegans Genotype: rax-2(bx131) III; bxIs14 him-5(e1490) V. Description: bxIs14 [pkd-2::GFP + pdx-1]. Ray axon defective. Mutagen: EMS Outcrossed: x>3 Made by: Lingyun Jia Received: 05/14/07 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM902 Species: Caenorhabditis elegans Genotype: madd-2(bx132) bxIs14 him-5(e1490) V. Description: bxIs14 [pkd-2::GFP + pdx-1]. Ray axon defective. Mutagen: EMS Outcrossed: x>3 Made by: Lingyun Jia Received: 05/14/07 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM905 Species: Caenorhabditis elegans Genotype: rax-5(bs137) II; bxIs14 him-5(e1490) V. Description: bxIs14 [pkd-2::GFP + pdx-1]. Ray axon defective. Mutagen: EMS Outcrossed: x>3 Made by: Lingyun Jia Received: 05/14/07 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM907 Species: Caenorhabditis elegans Genotype: rax-3(bx133) I; bxIs14 him-5(e1490) V. Description: bxIs14 [pkd-2::GFP + pdx-1]. Ray axon defective. Mutagen: EMS Outcrossed: x>3 Made by: Lingyun Jia Received: 05/14/07 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM908 Species: Caenorhabditis elegans Genotype: rax-3(bx138) I; bxIs14 him-5(e1490) V. Description: bxIs14 [pkd-2::GFP + pdx-1]. Ray axon defective. Mutagen: EMS Outcrossed: x>3 Made by: Lingyun Jia Received: 05/14/07 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM909 Species: Caenorhabditis elegans Genotype: rax-4(bx139) I; bxIs14 him-5(e1490) V. Description: bxIs14 [pkd-2::GFP + pdx-1]. Ray axon defective. Mutagen: EMS Outcrossed: x>3 Made by: Lingyun Jia Received: 05/14/07 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM911 Species: Caenorhabditis elegans Genotype: rax-6(bx140) IV; bxIs14 him-5(e1490) V. Description: bxIs14 [pkd-2::GFP + pdx-1]. Ray axon defective. Mutagen: EMS Outcrossed: x>3 Made by: Lingyun Jia Received: 05/14/07 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EM938 Species: Caenorhabditis elegans Genotype: pdfr-1(bx142) III; him-5(e1490) V. Description: Male leaving assay defective (Las), lethargic, hypereversal. Reference: Barrios, A, et al. Nat Neurosci. 2012 Dec;15(12):1675-82. Mutagen: EMS Outcrossed: x3 Made by: R. Ghosh & C. Fang Received: 05/06/13 Rutgers University, Piscataway, NJ ----------------------------------------------------------------------------- Strain: EM97 Species: Caenorhabditis elegans Genotype: ram-2(bx32) II; him-5(e1490) V. Description: Lumpy rays. Mutagen: EMS Outcrossed: x3 Made by: Baird S Received: 07/30/90 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: EN17 Species: Caenorhabditis elegans Genotype: catp-1(kr17) I. Description: DMPP resistant. Mos1 insertion. Mutagen: Mos1 Outcrossed: x6 Made by: AF Ruaud Received: 04/30/07 Universite Claude Bernard Lyon 1, Lyon, France ----------------------------------------------------------------------------- Strain: EN26 Species: Caenorhabditis elegans Genotype: lev-10(kr26::Mos1) I. Description: Weakly resistant to 1mM levamisole in acute screen. Animals become paralyzed after >30 min but nose remains hypercontracted. Sluggish Unc. pka lev-10. Mutagen: Mos1 Outcrossed: x2 Made by: Jean-Louis Bessereau Received: 02/10/05 Universite Claude Bernard Lyon 1, Lyon, France ----------------------------------------------------------------------------- Strain: EN5271 Species: Caenorhabditis elegans Genotype: (kr5271) I. Description: Mos1 transposon insertion in LG I. Presence of Mos1 can be detected using oligos oJL115 (Mos1 5'gctcaattcgcgccaaactatg3') and oVR261 (Chr I 5'gaaatagagggcagttcaacg3'). Reference: Giordano-Santini R, et al., (2010) Nature Methods. Aug 22. Mutagen: Mos1 Outcrossed: x0 Made by: Valerie Robert Received: 09/08/10 IECB (Bordeaux), Pessac, France ----------------------------------------------------------------------------- Strain: EN5273 Species: Caenorhabditis elegans Genotype: (kr5273) X. Description: Mos1 transposon insertion in LG X. Presence of Mos1 can be detected using oligos oJL115 (Mos1 5'gctcaattcgcgccaaactatg3') and oVR266 (Chr X 5'gacaaagacgtgtagttgcg3'). Reference: Giordano-Santini R, et al., (2010) Nature Methods. Aug 22. Mutagen: Mos1 Outcrossed: x0 Made by: Valerie Robert Received: 09/08/10 IECB (Bordeaux), Pessac, France ----------------------------------------------------------------------------- Strain: EN909 Species: Caenorhabditis elegans Genotype: krIs14 V. Description: krIs14 [hsp-16.48p::MosTransposase + lin-15B + unc-122p::GFP]. GFP expression in coelomocytes. Derived by integration of oxEx166. Reference: Toraason E, et al. STAR Protoc. 2021 Sep 8;2(3):100801. doi: 10.1016/j.xpro.2021.100801. PMID: 34527958. Mutagen: Outcrossed: x3x Made by: Bessereau lab Received: 02/06/25 University of Oregon, Eugene, OR ----------------------------------------------------------------------------- Strain: ENL19 Species: Caenorhabditis elegans Genotype: mir-58.1(n4640) IV; ctIs40 X. Description: ctIs40 [dbl-1(+) + sur-5::GFP]. Lon. dbl-1 over-expressiong from ctIs40. Mutagen: Outcrossed: x Made by: Encarnación Lozano Received: 01/08/15 Universidad Complutense de Madrid ----------------------------------------------------------------------------- Strain: ENL20 Species: Caenorhabditis elegans Genotype: mir-80(nDf53) III; daf-1(m213) mir-58.1(n4640) IV; mir-81&mir-82(nDf54) X. Description: Maintain at 15C. Temperature-sensitive dauer constitutive. Mutagen: Outcrossed: x Made by: Encarnación Lozano Received: 01/09/15 Universidad Complutense de Madrid ----------------------------------------------------------------------------- Strain: ENL21 Species: Caenorhabditis elegans Genotype: daf-2(e1370) mir-80(nDf53) III; mir-58.1(n4640) IV; mir-81&mir-82(nDf54) X. Description: Maintain at 15C. Temperature-sensitive dauer constitutive (daf-c). Mutagen: Outcrossed: x Made by: Encarnación Lozano Received: 02/16/15 Universidad Complutense de Madrid ----------------------------------------------------------------------------- Strain: ENL54 Species: Caenorhabditis elegans Genotype: daf-2(e1370) III; mir-58.1(n4640) IV; mir-81&mir-82(nDf54) X. Description: Maintain at 15C. Temperature-sensitive dauer constitutive. Mutagen: Outcrossed: x Made by: Encarnación Lozano Received: 01/09/15 Universidad Complutense de Madrid ----------------------------------------------------------------------------- Strain: ENL55 Species: Caenorhabditis elegans Genotype: daf-2(e1370) mir-80(nDf53) III; mir-81&mir-82(nDf54) X. Description: Maintain at 15C. Temperature-sensitive dauer constitutive. Mutagen: Outcrossed: x Made by: Encarnación Lozano Received: 01/09/15 Universidad Complutense de Madrid ----------------------------------------------------------------------------- Strain: ENL59 Species: Caenorhabditis elegans Genotype: sma-10(ok2224) IV; dbl-1(nk3) V. Description: Derived from RB1739 and NU3. Mutagen: Outcrossed: x Made by: Encarnación Lozano Received: 02/16/15 Universidad Complutense de Madrid ----------------------------------------------------------------------------- Strain: ENL60 Species: Caenorhabditis elegans Genotype: sma-10(ok2224) IV; ctIs40 X. Description: ctIs40 [dbl-1(+) + sur-5::GFP]. Mutagen: Outcrossed: x Made by: Encarnación Lozano Received: 02/16/15 Universidad Complutense de Madrid ----------------------------------------------------------------------------- Strain: ENL61 Species: Caenorhabditis elegans Genotype: mir-58.1(n4640) IV; dbl-1(nk3) V. Description: Small. Derived from MT15024 and NU3. Mutagen: Outcrossed: x Made by: Encarnación Lozano Received: 02/16/15 Universidad Complutense de Madrid ----------------------------------------------------------------------------- Strain: ENL62 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; sma-10(ok2224) IV. Description: Derived from RB1739 and CF1038. Mutagen: Outcrossed: x Made by: Encarnación Lozano Received: 02/16/15 Universidad Complutense de Madrid ----------------------------------------------------------------------------- Strain: ENL63 Species: Caenorhabditis elegans Genotype: daf-16(mgDf47) I; sma-10(ok2224) IV; xrIs87. Description: xrIs87 [daf-16(alpha)::GFP::daf-16B + rol-6(su1006)]. Rollers. Derived from RB1739 and GR1352. Mutagen: Outcrossed: x Made by: Encarnación Lozano Received: 02/16/15 Universidad Complutense de Madrid ----------------------------------------------------------------------------- Strain: ENL64 Species: Caenorhabditis elegans Genotype: sma-10(ok2224) IV; fshr-1(ok778) V. Description: Derived from RB1739 and RB911. Mutagen: Outcrossed: x Made by: Encarnación Lozano Received: 02/16/15 Universidad Complutense de Madrid ----------------------------------------------------------------------------- Strain: ENL65 Species: Caenorhabditis elegans Genotype: sma-10(ok2224) IV; sek-1(km4) X. Description: Derived from RB1739 and KU4. Mutagen: Outcrossed: x Made by: Encarnación Lozano Received: 02/16/15 Universidad Complutense de Madrid ----------------------------------------------------------------------------- Strain: ENL66 Species: Caenorhabditis elegans Genotype: eat-2(ad465) II; sma-10(ok2224) IV. Description: Derived from RB1739 and DA465. Mutagen: Outcrossed: x Made by: Encarnación Lozano Received: 02/16/15 Universidad Complutense de Madrid ----------------------------------------------------------------------------- Strain: ENL67 Species: Caenorhabditis elegans Genotype: daf-16(mgDf47) I; sma-10(ok2224) IV; muIs61. Description: muIs61 [(pKL78) daf16::GFP + rol-6(su1006)]. muIs61 rescues daf-16(mu86). muIs61 is a gamma-induced insertion of muEx50. Derived from RB1739 and CF1139. Mutagen: Outcrossed: x Made by: Encarnación Lozano Received: 02/16/15 Universidad Complutense de Madrid ----------------------------------------------------------------------------- Strain: ENL68 Species: Caenorhabditis elegans Genotype: sma-10(ok2224) zIs356 IV. Description: zIs356 [daf-16p::daf-16a/b::GFP + rol-6(su1006)]. See strain TJ356 for additional information about zIs356. Derived from RB1739 and TJ356. Mutagen: Outcrossed: x Made by: Encarnación Lozano Received: 02/16/15 Universidad Complutense de Madrid ----------------------------------------------------------------------------- Strain: ENL69 Species: Caenorhabditis elegans Genotype: tol-1(nr2033) I; sma-10(ok2224) IV. Description: Derived from RB1739 and IG10. Mutagen: Outcrossed: x Made by: Encarnación Lozano Received: 02/16/15 Universidad Complutense de Madrid ----------------------------------------------------------------------------- Strain: ENL70 Species: Caenorhabditis elegans Genotype: daf-2(e1370) III; sma-10(ok2224) IV. Description: Derived from RB1739 and CB1370. Mutagen: Outcrossed: x Made by: Encarnación Lozano Received: 02/16/15 Universidad Complutense de Madrid ----------------------------------------------------------------------------- Strain: ER10 Species: Caenorhabditis elegans Genotype: unc-123(jd5) III. Description: Temperature sensitive, semidominant mutant that is Unc when moving backward. Dorsal muscular contraction is stronger than that of ventral, resulting in an asymmetric pattern of locomotion in which the animal either forms a dorsal coil or moves in circles with the dorsal side central to the circle. Most likely a gain of function allele of sup-1. Mutagen: EMS Outcrossed: x5 Made by: Ghandi/Walthall Received: 05/16/94 Georgia State University, Atlanta ----------------------------------------------------------------------------- Strain: ER50 Species: Caenorhabditis elegans Genotype: unc-123(jd5jd10) III. Description: Recessive suppressor of unc-123(jd5). Exhibits a subtle dorsal asymmetric locomotory defect as a homozygote when grown at 25C. Dominant suppressor of unc-17(e245). Most likely unc-123 is allelic with sup-1. Mutagen: EMS Outcrossed: x5 Made by: Li/Walthall Received: 05/16/94 Georgia State University, Atlanta ----------------------------------------------------------------------------- Strain: ERC102 Species: C. elegans Genotype: ieSi57 II; smc-3(syb5520[smc-3::GGGGS::AID::emGFP]) III. Description: ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Single copy transgene inserted into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in the soma. Degron and emGFP tag inserted into endogenous smc-3 locus. Derived by crossing parental strains PHX5520 with CA1200. Reference: https://www.biorxiv.org/content/10.1101/2023.09.18.558239v1. Mutagen: Outcrossed: x1 Made by: Jun Kim Received: 10/23/23 New York University, New York, NY ----------------------------------------------------------------------------- Strain: ERC103 Species: C. elegans Genotype: ieSi57 II; wapl-1(syb6035[wapl-1::GGGGS::AID::emGFP]) IV. Description: ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Single copy transgene inserted into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in the soma. Degron and emGFP tag inserted into endogenous smc-3 locus. Derived by crossing parental strains PHX6035 with CA1200. Reference: https://www.biorxiv.org/content/10.1101/2023.09.18.558239v1. Mutagen: Outcrossed: x1 Made by: Jun Kim Received: 10/23/23 New York University, New York, NY ----------------------------------------------------------------------------- Strain: ERC82 Species: C. elegans Genotype: ieSi57 II; ers54[dpy-27::degron::GFP] III. Description: ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Degron::GFP tag inserted into the endogenous dpy-27 locus. Dumpy, Him, X chromosome dosage compensation hypomorph. ieSi57 is a single-copy transgene insertion into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in the soma. This strain can be used for auxin-inducible degradation (AID) of target proteins in somatic tissues. Reference: Morao AK, et al. Mol Cell. 2022 Nov 17;82(22):4202-4217.e5. doi: 10.1016/j.molcel.2022.10.002. PMID: 36302374. Mutagen: Outcrossed: x0 Made by: Ana Morao Received: 12/07/22 New York University, New York, NY ----------------------------------------------------------------------------- Strain: ERC83 Species: C. elegans Genotype: ieSi57 ers55[top-2::degron::GFP] II. Description: ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Degron tag inserted into the endogenous top-2 locus. ieSi57 is a single-copy transgene insertion into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in the soma. This strain can be used for auxin-inducible degradation (AID) of target proteins in somatic tissues. Reference: Morao AK, et al. Mol Cell. 2022 Nov 17;82(22):4202-4217.e5. doi: 10.1016/j.molcel.2022.10.002. PMID: 36302374. Mutagen: Outcrossed: x0 Made by: Ana Morao Received: 12/07/22 New York University, New York, NY ----------------------------------------------------------------------------- Strain: ERC84 Species: C. elegans Genotype: top-1(ers56[top-1::degron::GFP]) I; ieSi57 II. Description: ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Degron tag inserted at the end of exon five in the endogenous top-1 locus. ieSi57 is a single-copy transgene insertion into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in the soma. This strain can be used for auxin-inducible degradation (AID) of target proteins in somatic tissues. Reference: Morao AK, et al. Mol Cell. 2022 Nov 17;82(22):4202-4217.e5. doi: 10.1016/j.molcel.2022.10.002. PMID: 36302374. Mutagen: Outcrossed: x0 Made by: Ana Morao Received: 12/07/22 New York University, New York, NY ----------------------------------------------------------------------------- Strain: ERS100 Species: C. elegans Genotype: eraIs1. Description: eraIs1 [dat-1p::mCherry + dat-1p::hSNCA::Venus]. Inclusions of human alpha-Synuclein::Venus in dopaminergic neurons with marked with mCherry. Overexpression of human SNCA (alpha-Synuclein tagged with Venus) causes degenerative signs in dopaminergic neurons. Reference: Vozdek R, et al. 2022 Apr 27:14:806000. doi: 10.3389/fnagi.2022.806000. PMID: 35572147. Mutagen: integrated by UV Outcrossed: x5 Made by: Roman Vozdek Received: 02/12/24 Eurac research ----------------------------------------------------------------------------- Strain: ERT16 Species: Caenorhabditis elegans Genotype: jyEx4. Description: jyEx4 [zip-2::GFP + myo-2p::mCherry]. Pick animals with mCherry+ pharynx to maintain. Array is stable; might have integrated. ZIP-2::GFP protein is only expressed in the intestine when animals are infected with pathogenic Pseudomonas aeruginosa strain (PA14) or when other processes (e.g. translation) are perturbed (see Dunbar TL, et al. for more information). Reference: Dunbar TL, et al. Cell Host Microbe. 2012 Apr 19;11(4):375-86. Mutagen: Outcrossed: x0 Made by: Emily Troemel Received: 01/07/14 UCSD, La Jolla CA, USA ----------------------------------------------------------------------------- Strain: ERT20 Species: Caenorhabditis elegans Genotype: jyEx6. Description: jyEx6 [zip-2::GFP + myo-2p::mCherry]. Pick animals with mCherry+ pharynx to maintain. zip-2p::GFP reporter is constitutively on. Reference: Dunbar TL, et al. Cell Host Microbe. 2012 Apr 19;11(4):375-86. Mutagen: Outcrossed: x0 Made by: Emily Troemel Received: 01/07/14 UCSD, La Jolla CA, USA ----------------------------------------------------------------------------- Strain: ERT54 Species: C. elegans Genotype: jyIs8 X. Description: jyIs8 [pals-5p::GFP + myo-2p::mCherry] X. GFP expression induced in the intestine after intracellular infection or proteasomal inhibition. Reference: Bakowski MA, et al. PLoS Pathog. 2014 Jun 19;10(6):e1004200. Mutagen: Outcrossed: x3 Made by: Emily Troemel Received: 12/19/17 UCSD, La Jolla CA, USA ----------------------------------------------------------------------------- Strain: ERT60 Species: C. elegans Genotype: jyIs13 II. Description: jyIs13 [act-5p::GFP::ACT-5 + rol-6(su1006)] II. Rollers with GFP localized to apical side of intestine. Reference: Szumowski SC, et al. Cell Microbiol. 2015 Jul 6. PMID:26147591. Mutagen: Outcrossed: x3 Made by: Suzy Szumowski Received: 12/19/17 UCSD, La Jolla CA, USA ----------------------------------------------------------------------------- Strain: ERT691 Species: C. elegans Genotype: rcs-1(jy84) X. Description: Full CRISPR deletion of rcs-1 via CRISPR in a dpy-10 background, outcrossed 3x to wild-type. Superficially wild-type. Reference: Panek J, et al. Proc Natl Acad Sci USA. .2020 Apr 7;117(14):7950-7960. doi: 10.1073/pnas.1918417117. PMID: 32193347. Mutagen: CRISPR Outcrossed: x3 Made by: Johan Panek Received: 01/24/24 UCSD, La Jolla CA, USA ----------------------------------------------------------------------------- Strain: ERT848 Species: C. elegans Genotype: fbxa-75(jy143) III. Description: Full CRISPR deletion of fbxa-75 via CRISPR in a dpy-10 background, outcrossed 3x to wild-type. Superficially wild-type. Reference: Panek J, et al. Proc Natl Acad Sci USA. .2020 Apr 7;117(14):7950-7960. doi: 10.1073/pnas.1918417117. PMID: 32193347. Mutagen: CRISPR Outcrossed: x3 Made by: Spencer Gang Received: 01/24/24 UCSD, La Jolla CA, USA ----------------------------------------------------------------------------- Strain: ERT852 Species: C. elegans Genotype: fbxa-158(jy145) II. Description: Full CRISPR deletion of fbxa-158 via CRISPR in a dpy-10 background, outcrossed 3x to wild-type. Superficially wild-type. Reference: Panek J, et al. Proc Natl Acad Sci USA. .2020 Apr 7;117(14):7950-7960. doi: 10.1073/pnas.1918417117. PMID: 32193347. Mutagen: CRISPR Outcrossed: x3 Made by: Spencer Gang Received: 01/24/24 UCSD, La Jolla CA, USA ----------------------------------------------------------------------------- Strain: ESC332 Species: Caenorhabditis elegans Genotype: rpoa-2(cse319[degron::GFP::rpoa-2]) I; ieSi57 II. Description: ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Maintain at 15-20C. Degron and GFP tag inserted at the N-terminus of the endogenous rpoa-2 locus. This strain can be used for auxin-inducible degradation (AID) of RPOA-2 in somatic tissues. Reference: Zhao Q, et al. PLoS Biol. 2023 Aug 31;21(8):e3002276. doi: 10.1371/journal.pbio.3002276. PMID: 37651423. Mutagen: CRIPSR/Cas9 Outcrossed: x2 Made by: Qiuxia Zhao Received: 02/05/24 University of Texas, Austin, TX ----------------------------------------------------------------------------- Strain: ESC351 Species: Caenorhabditis elegans Genotype: rpoa-2(cse319[degron::GFP::rpoa-2]) I; reSi2 II. Description: reSi2 [col-10p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (II:0.77). Maintain at 15-20C. Degron and GFP tag inserted at the N-terminus of the endogenous rpoa-2 locus. This strain can be used for auxin-inducible degradation (AID) of RPOA-2 in hypodermis. Reference: Zhao Q, et al. PLoS Biol. 2023 Aug 31;21(8):e3002276. doi: 10.1371/journal.pbio.3002276. PMID: 37651423. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Qiuxia Zhao Received: 02/26/24 University of Texas, Austin, TX ----------------------------------------------------------------------------- Strain: ESC352 Species: Caenorhabditis elegans Genotype: qzIs15[rpoa-2p::degron::GFP::rpoa-2] I; ieSi60 II. Description: ieSi60 [myo-2p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Maintain at 15-20C. Degron and GFP tag inserted at the N-terminus of the endogenous rpoa-2 locus. This strain can be used for auxin-inducible degradation (AID) of RPOA-2 in pharyngeal muscle. Reference: Zhao Q, et al. PLoS Biol. 2023 Aug 31;21(8):e3002276. doi: 10.1371/journal.pbio.3002276. PMID: 37651423. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Qiuxia Zhao Received: 02/05/24 University of Texas, Austin, TX ----------------------------------------------------------------------------- Strain: ESC360 Species: Caenorhabditis elegans Genotype: rpoa-2(cse319[degron::GFP::rpoa-2]) I; emcSi71 IV. Description: emcSi71 [myo-3p::TIR1::mRuby] (IV: -0.05). Maintain at 15-20C. Degron and GFP tag inserted at the N-terminus of the endogenous rpoa-2 locus. This strain can be used for auxin-inducible degradation (AID) of RPOA-2 in muscles. Reference: Zhao Q, et al. PLoS Biol. 2023 Aug 31;21(8):e3002276. doi: 10.1371/journal.pbio.3002276. PMID: 37651423. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Qiuxia Zhao Received: 02/05/24 University of Texas, Austin, TX ----------------------------------------------------------------------------- Strain: ESC373 Species: Caenorhabditis elegans Genotype: rpoa-2(cse319[degron::GFP::rpoa-2]) I; ieSi61 II. Description: ieSi61 [ges-1p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Maintain at 15-20C. Degron and GFP tag inserted at the N-terminus of the endogenous rpoa-2 locus. This strain can be used for auxin-inducible degradation (AID) of RPOA-2 in the intestine. Reference: Zhao Q, et al. PLoS Biol. 2023 Aug 31;21(8):e3002276. doi: 10.1371/journal.pbio.3002276. PMID: 37651423. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Qiuxia Zhao Received: 02/05/24 University of Texas, Austin, TX ----------------------------------------------------------------------------- Strain: ESC374 Species: Caenorhabditis elegans Genotype: rpoa-2(cse319[degron::GFP::rpoa-2]) I; ieSi38 IV. Description: ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. Maintain at 15-20C. Degron and GFP tag inserted at the N-terminus of the endogenous rpoa-2 locus. This strain can be used for auxin-inducible degradation (AID) of RPOA-2 in germ line and early embryos. Reference: Zhao Q, et al. PLoS Biol. 2023 Aug 31;21(8):e3002276. doi: 10.1371/journal.pbio.3002276. PMID: 37651423. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Qiuxia Zhao Received: 02/05/24 University of Texas, Austin, TX ----------------------------------------------------------------------------- Strain: ESC716 Species: Caenorhabditis elegans Genotype: ida-1(cse716[ida-1:: wrmScarlet]) III. Description: mScarlet tag inserted into the C-terminus of the endogenous ida-1 locus. Maintain at 16-20C. Reference: Zhao Q, et al. PLoS Biol. 2023 Aug 31;21(8):e3002276. doi: 10.1371/journal.pbio.3002276. PMID: 37651423. Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Qiuxia Zhao Received: 02/05/25 University of Texas, Austin, TX ----------------------------------------------------------------------------- Strain: ESK1 Species: C. elegans Genotype: unc-119(ed3) III; aak-2(ok524) X; fphIs1. Description: fphIs1 [aak-2Ap::aak-2A::GFP + unc-119(+)]. aak-2A isoform expressed with its own promoter in aak-2(ok524) background. Reference: Jeong JH, et al. Nat Commun. 2023 Jan 18;14(1):288. doi: 10.1038/s41467-023-35952-z. PMID: 36653384. Mutagen: Outcrossed: x4 Made by: Eun-Soo Kwon Received: 11/17/23 Korea Research Inst of Bioscience & Biotech, Daejeon, South Korea ----------------------------------------------------------------------------- Strain: ESK2 Species: C. elegans Genotype: unc-119(ed3) III; aak-2(ok524) X; fphEx1. Description: fphEx1 [aak-2Cp::aak-2C::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. aak-2C isoform expressed from its own promoter in aak-2(ok524) background. Reference: Jeong JH, et al. Nat Commun. 2023 Jan 18;14(1):288. doi: 10.1038/s41467-023-35952-z. PMID: 36653384. Mutagen: Outcrossed: x4 Made by: Eun-Soo Kwon Received: 11/17/23 Korea Research Inst of Bioscience & Biotech, Daejeon, South Korea ----------------------------------------------------------------------------- Strain: ESK3 Species: C. elegans Genotype: unc-119(ed3) III; aak-2(ok524) X; fphEx2. Description: fphEx2 [aak-2A/Cp::aak-2A/C::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. aak-2A and aak-2C isoforms expressed from their own promoter in aak-2(ok524) background. Reference: Jeong JH, et al. Nat Commun. 2023 Jan 18;14(1):288. doi: 10.1038/s41467-023-35952-z. PMID: 36653384. Mutagen: Outcrossed: x4 Made by: Eun-Soo Kwon Received: 11/17/23 Korea Research Inst of Bioscience & Biotech, Daejeon, South Korea ----------------------------------------------------------------------------- Strain: ESK4 Species: C. elegans Genotype: unc-119(ed3) III; aak-2(ok524) X; fphEx3. Description: fphEx3 [aak-2Ap::aak-2C::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. aak-2c isoform expressed from the aak-2a promoter in aak-2(ok524) background. Reference: Jeong JH, et al. Nat Commun. 2023 Jan 18;14(1):288. doi: 10.1038/s41467-023-35952-z. PMID: 36653384. Mutagen: Outcrossed: x4 Made by: Eun-Soo Kwon Received: 11/17/23 Korea Research Inst of Bioscience & Biotech, Daejeon, South Korea ----------------------------------------------------------------------------- Strain: ESK5 Species: C. elegans Genotype: unc-119(ed3) III; aak-2(ok524) X; fphIs2. Description: fphIs2 [unc-119p::aak-2A::GFP + unc-119(+)]. aak-2A isoform expressed from the neuronal unc-119 promoter in aak-2(ok524) background. Reference: Jeong JH, et al. Nat Commun. 2023 Jan 18;14(1):288. doi: 10.1038/s41467-023-35952-z. PMID: 36653384. Mutagen: Outcrossed: x4 Made by: Eun-Soo Kwon Received: 11/17/23 Korea Research Inst of Bioscience & Biotech, Daejeon, South Korea ----------------------------------------------------------------------------- Strain: ESK6 Species: C. elegans Genotype: unc-119(ed3) III; aak-2(ok524) X; fphIs3. Description: fphIs3 [rab-3p::aak-2A::GFP + unc-119(+)]. aak-2A isoform expressed from the neuronal rab-3 promoter in aak-2(ok524) background. Reference: Jeong JH, et al. Nat Commun. 2023 Jan 18;14(1):288. doi: 10.1038/s41467-023-35952-z. PMID: 36653384. Mutagen: Outcrossed: x4 Made by: Eun-Soo Kwon Received: 11/17/23 Korea Research Inst of Bioscience & Biotech, Daejeon, South Korea ----------------------------------------------------------------------------- Strain: ESK7 Species: C. elegans Genotype: unc-119(ed3) III; aak-2(ok524) X; fphEx4. Description: fphEx4 [vha-6p::aak-2A::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. aak-2A isoform expressed from the intestinal vha-6 promoter in aak-2(ok524) background. Reference: Jeong JH, et al. Nat Commun. 2023 Jan 18;14(1):288. doi: 10.1038/s41467-023-35952-z. PMID: 36653384. Mutagen: Outcrossed: x4 Made by: Eun-Soo Kwon Received: 11/17/23 Korea Research Inst of Bioscience & Biotech, Daejeon, South Korea ----------------------------------------------------------------------------- Strain: ET100 Species: Caenorhabditis elegans Genotype: H01G02.2(ok200) IV. Description: H01G02.2. External left primer: TGCATCCCTTTGATTCCTTC. External right primer: AAACCTGGGCGCTTTTATTT. Internal left primer: GCAATCCTTGCTTGATCCAT. Internal right primer: TGATTGCAACGTTCCATGAT. Internal WT amplicon: 3033 bp. Deletion size: 1251 bp. Deletion left flank: AAACTCACTTTTGAAACATTCGGGACCATT. Deletion right flank: GATGAAGATCATGGAACGTTGCAATCAATT. This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. URL: http://www.celeganskoconsortium.omrf.org. Mutagen: UV/TMP Outcrossed: x6 Made by: Edward Kipreos Received: 07/21/03 University of Georgia, Athens, GA ----------------------------------------------------------------------------- Strain: ET113 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; ekIs2. Description: ekIs2 contains [pie-1p::GFP::cyb-1 + unc-119(+)]. Translational fusion of CYB-1 expressed from the pie-1 promoter and including the pie-1 3'UTR. GFP::CYB-1 expression in the proximal gonad, with staining disappearing in the zygote. Maintain at 25°C. Mutagen: Outcrossed: x Made by: Edward Kipreos Received: 09/29/09 University of Georgia, Athens, GA ----------------------------------------------------------------------------- Strain: ET137 Species: Caenorhabditis elegans Genotype: C30G12.1(ok910) II. Description: C30G12.1 Homozygous. No overt morphological or behavioral abnormalities. 1286 bp deletion. The region of cosmid C30G12 that is deleted is 39,238 - 40,523 bps (inclusive). The deletion includes an ectopic 5 bp sequence, GGTTA. The sequence crossing the deletion for ok910 is: ....GCCATGGTTAAAAGT GGTTA AAAAATTCAGTATAT... This deletion was generated by the C. elegans Gene Knockout Project at OMRF, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publication resulting from its use. http://www.mutantfactory.ouhsc.edu/ Mutagen: UV/TMP Outcrossed: x1 Made by: Edward Kipreos Received: 09/26/03 University of Georgia, Athens, GA ----------------------------------------------------------------------------- Strain: ET182 Species: Caenorhabditis elegans Genotype: ekEx25. Description: ekEx25 [wrt-2p::CDC-6::GFP + rol-6(su1006)]. Pick Rollers to maintain the extrachromosomal array. Mutagen: Outcrossed: x Made by: Jihyun Kim Received: 12/22/08 University of Georgia, Athens, GA ----------------------------------------------------------------------------- Strain: ET263 Species: Caenorhabditis elegans Genotype: ddb-1(tm1769)/dpy-20(e2017) IV. Description: Heterozygotes are WT and segregate WT, Dpy and ddb-1 homozygotes, which arrest as L2-stage larvae (approx. 15%) or become sterile adults with protruding vulva (approx. 85%). Mutagen: UV/TMP Outcrossed: x6 Made by: S. Mitani/E. Kipreos Received: 04/26/07 University of Georgia, Athens, GA ----------------------------------------------------------------------------- Strain: ET507 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; cki-2(ok2105) II; glp-1(ar202) III. Description: Temperature-sensitive. Maintain at 15C. Animals form germline tumors that prevent fertility at restrictive temerature (25C). This is the first strain reported to be used for the isolation of germ cells for in vitro culture. This strain allows germ cells to remain viable for longer periods than other tumorous mutant strains tested. Reference: Chaudhari SH, et al. Dev Cell. 2016 Jul 11;38(1):33-46. Mutagen: Outcrossed: x Made by: Edward T. Kipreos Received: 01/10/17 University of Georgia, Athens, GA ----------------------------------------------------------------------------- Strain: ET65 Species: Caenorhabditis elegans Genotype: cul-2(ek1)/unc-64(e246) III. Description: Heterozygotes are WT and segregate WT, Unc and adult steriles (cul-2 homozygotes). cul-2 homozygotes have large germ cells and lay few eggs (slightly temperature sensitive, with more eggs at lower temperatures) that arrest early in development with multiple nuclei. Mutagen: EMS Outcrossed: x5 Made by: Edward T. Kipreos Received: 02/07/00 University of Georgia, Athens, GA ----------------------------------------------------------------------------- Strain: EU1 Species: Caenorhabditis elegans Genotype: skn-1(zu67) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Unc, WT which give only dead eggs, and dead eggs. Maintain by picking Unc. Mutagen: EMS Outcrossed: x8 Made by: Bruce Bowerman Received: 01/10/94 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: EU1004 Species: Caenorhabditis elegans Genotype: tac-1(or402) II. Description: Homozygous mutant animals give rise to >97% dead embyros at the restrictive temperature of 26C, with embryos exhibiting a lack of pronuclear migration at the one-cell stage. Homozygous mutant animals give rise to 50% dead embryos at the permissive temperature of 15C. Maintain at 15C. Mutagen: EMS Outcrossed: x2 Made by: Jennifer Phillips Received: 05/15/08 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: EU1006 Species: Caenorhabditis elegans Genotype: dnc-1(or404) IV. Description: Temperature sensitive. At 15C, 100% viable. At 26C, 2% viable. At 25C, 10% viable. Missense mutation R to C at position 1237 (nucleotide #3709: cgt to tgt). Reduced brood size after upshift. Received new stock 12/04. Mutagen: EMS Outcrossed: x4 Made by: John Willis Received: 05/10/04 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1007 Species: Caenorhabditis elegans Genotype: mel-26(or543) I. Description: Temperature sensitive. Maintain at 15C. At 25C, hermaphrodites lay embryos that die. Transverse PO mitotic spindle, cytokinesis defects. Missense mutation at nucleotide #379 (cyt->tgt); amino acid #127 (R to C). Mutagen: EMS Outcrossed: x4 Made by: John Willis Received: 01/13/04 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1008 Species: Caenorhabditis elegans Genotype: apo-5(or358) I. Description: Temperature sensitive, embryonic lethal mutant. 98% of embryos produced by homoygous mothers hatch at 15C; 0% produced at 26C hatch (but some viability at 25C). Mutation is recessive at 26C. First mitotic spindle in one-cell zygote is mis-positioned, often abnormally near the posterior pole; some chromosome segregation defects. Mutagen: EMS Outcrossed: x4 Made by: Sandra Encalada Received: 01/26/06 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1062 Species: Caenorhabditis elegans Genotype: sur-6(or550) I. Description: Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. Mutagen: EMS Outcrossed: x4 Made by: Bruce Nash Received: 11/15/11 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1065 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; orIs1. Description: Appears WT. orIs1 [pie-1p::GFP::mei-1 + unc-119(+)]. Maintain at 20C or above. Below 20C the GFP signal turns off. Mutagen: Outcrossed: x Made by: Thimo Kurz Received: 07/03/03 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1068 Species: Caenorhabditis elegans Genotype: unc-119(ed3) ruIs32 III; repo-1 (or430) IV; ruIs57. Description: ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. ruIs57 [pie-1p::GFP::tubulin + unc-119(+)]. Maintain at 15C. Temperature-sensitive embryonic-lethal at restrictive temperature of 26C; viable at permissive temperature of 15C. Reversed AP polarity axis at restrictive temperature with his-11::GFP and tubulin::GFP expression. Reference: Keikhaee MR, et al. PLoS One. 2014 Sep 4;9(9):e106484. Mutagen: ENU Outcrossed: x5 Made by: EB Nash Received: 10/10/14 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1073 Species: Caenorhabditis elegans Genotype: him-8(e1489) IV; act-2(or295) V. Description: Weakly semi-dominant, temperature sensitive, embryonic lethal mutant. 38% of embyros produced by homozygous mothers at 15C will hatch; 2% produced at 26C will hatch. Mutation is semi-dominant at 26C: 12% of embryos produced by heterozygous mothers hatched at 26C, and about 25% of the survivors were or295/or295, indicating the lethality is semi-dominant and maternal. Mutant embyros exhibit excess cortical actomyosin contractility (extra furrows and protrusions) in early embryonic cells, during interphase and most of mitosis. Throws males. or295 is a gga to aga substitution (G16R). Mutagen: EMS Outcrossed: x7 Made by: John Willis Received: 01/26/06 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1074 Species: Caenorhabditis elegans Genotype: gpr-1(or574) III. Description: Temperature-sensitive embryonic-lethal mutant. Maintain at 15C. Embryonic lethal at restrictive temperature of 26C; viable embryos at permissive temperature of 15C. [NOTE: PCR detection of or574 can be difficult. gpr-1 and gpr-2 paralogues are closely linked and 97% identical in DNA sequence.] Reference: Couwenbergs C, et al. J Cell Biol. 2007 Oct 8;179(1):15-22. Mutagen: ENU Outcrossed: x5 Made by: Morgan Goulding Received: 09/19/14 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1077 Species: Caenorhabditis elegans Genotype: mel-26(or543) I; him-8(e1489) IV. Description: Temperature sensitive. Maintain at 15C. At 25C, hermaphrodites lay embryos that die. Transverse PO mitotic spindle, cytokinesis defects. Missense mutation at nucleotide #379 (cyt->tgt); amino acid #127 (R to C). Throws males. Mutagen: EMS Outcrossed: x4 Made by: John Willis Received: 01/13/04 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1133 Species: Caenorhabditis elegans Genotype: apo-5(or358) ruIs32 III Description: ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. apo-5(or358) is temperature-sensitive embryonic lethal. 100% dead embryos when L4 larvae are shifted to 26.6C. A small percentage of embryos survive form L4 larvae shifted to 25-26C. or358 appears to be semi-dominant; L4 or358/+ heterozygotes shifted to 26C produce ~20% embryonic lethality. Maintain at 15C. References: Encalada SE, et al., Mol Biol Cell. 2005 Mar;16(3):1056-70. Strome S, et al. Mol Biol Cell. 2001 Jun;12(6):1751-64. Mutagen: EMS Outcrossed: x4 Made by: Sandra Encalada Received: 04/06/11 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1135 Species: Caenorhabditis elegans Genotype: tba-1(or346) I. Description: Dominant, conditional, maternal-effect. At 15C, almost all embryos hatch from or346/+ and or346/or346 mothers. At25C, about 85% of embryos die from or346/+ mothers and about 100% of embryos die from or346/or346 mothers. Mutagen: EMS Outcrossed: x4 Made by: Rebecca Lyczak Received: 09/22/04 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1161 Species: Caenorhabditis elegans Genotype: tba-1(or594) I. Description: Semi-dominant. Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. Mutagen: ENU Outcrossed: x1 Made by: Aleena Garner Received: 04/10/12 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1172 Species: Caenorhabditis elegans Genotype: zyg-9(or593) II. Description: Temperature sensitive. Maintain at 15C. At 26C, animals give rise to 99% dead embryos, with embryos exhibiting lack of pronuclear migration at the one-cell stage. At 15C, animals give rise to 73% dead embryos. Mutagen: EMS Outcrossed: x1 Made by: James Clayton Carter Received: 12/03/08 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: EU1257 Species: Caenorhabditis elegans Genotype: dnc-1(or676) IV. Description: Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. Mutagen: ENU Outcrossed: x1 Made by: Clayton Carter Received: 11/15/11 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1295 Species: Caenorhabditis elegans Genotype: act-2(or621) V. Description: Strongly semi-dominant, temperature sensitive, embryonic lethal mutant. 40% of embyros produced by homozygous mothers at 15C will hatch; 0% produced at 26C will hatch. Mutation is semi-dominant at 26C: 85% of embyros produced by heterozygous mothers hatched at 26C. Mutant embryos exhibit excess coritical actomyosin contractility (extra furrows and protrusions) in early embryonic cells; during interphase and most of mitosis. or621 is a tcc to gcc substitution (S15A). Mutagen: ENU Outcrossed: x4 Made by: John Willis Received: 01/26/06 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1296 Species: Caenorhabditis elegans Genotype: him-8(e1489) IV; act-2(or621) V. Description: Strongly semi-dominant, temperature sensitive, embryonic lethal mutant. 40% of embyros produced by homozygous mothers at 15C will hatch; 0% produced at 26C will hatch. Mutation is semi-dominant at 26C: 85% of embyros produced by heterozygous mothers hatched at 26C. Mutant embryos exhibit excess coritical actomyosin contractility (extra furrows and protrusions) in early embryonic cells; during interphase and most of mitosis. Throws males. or621 is a tcc to gcc substitution (S15A). Mutagen: ENU Outcrossed: x4 Made by: John Willis Received: 01/26/06 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1303 Species: C elegans Genotype: cyk-4(or570) III. Description: Maintain at 15C. Temperature-sensitive embryonic lethal with cytokinesis defects. Reference: Canman JC, et al. Science. 2008 Dec 5;322(5907):1543-6. Mutagen: EMU Outcrossed: x6 Made by: Julie C. Canman Received: 04/23/19 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1327 Species: Caenorhabditis elegans Genotype: par-2(or640) III. Description: Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. Mutagen: ENU Outcrossed: x3 Made by: Rebecca Luczak Received: 11/15/11 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1347 Species: Caenorhabditis elegans Genotype: spd-2(or655) I. Description: Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. Mutagen: ENU Outcrossed: x1 Made by: Julie Canman Received: 11/15/11 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1355 Species: Caenorhabditis elegans Genotype: zyg-9(or628) II. Description: Temperature sensitive. Maintain at 15C. At 26C, animals give rise to 100% dead embryos, with embryos exhibiting lack of pronuclear migration at the one-cell stage. At 15C, animals give rise to 42% dead embryos. Mutagen: EMS Outcrossed: x1 Made by: James Clayton Carter Received: 12/03/08 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: EU1381 Species: Caenorhabditis elegans Genotype: act-2(or295) V. Description: Weakly semi-dominant, temperature sensitive, embryonic lethal mutant. 38% of embyros produced by homozygous mothers at 15C will hatch; 2% produced at 26C will hatch. Mutation is semi-dominant at 26C: 12% of embryos produced by heterozygous mothers hatched at 26C, and about 25% of the survivors were or295/or295, indicating the lethality is semi-dominant and maternal. Mutant embyros exhibit excess cortical actomyosin contractility (extra furrows and protrusions) in early embryonic cells, during interphase and most of mitosis. or295 is a gga to aga substitution (G16R). Mutagen: EMS Outcrossed: x8 Made by: John Willis Received: 01/26/06 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1382 Species: Caenorhabditis elegans Genotype: orEx1. Description: orEx1[act-2 promoter::GFP::act-2 genomic fusion + rol-6(su1006)]. Shows cytoplasmic and cortical ACT-2::GFP expression in early embryonic cells, in epidermal cells during embryonic elongation, and in body wall muscle cells and some neurons in adults. All expression is zygotic; no germline expression is detected for this trangene even though act-2 is maternally expressed. Maintain by picking Rollers. Mutagen: Outcrossed: x0 Made by: John Willis Received: 01/26/06 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1383 Species: Caenorhabditis elegans Genotype: act-2(ok1229) V. Description: Strain is homozygous viable due to redundancy of act- and act-3 genes. AT 15C, all embyros produced by homozygous mothers hatch; at 26C, 88% of embryos hatch. Deletion which removes 544 nucleotides of act-2 plus the predicted 3' UTR and 705 nucleotides 3' of that. This removes 163/376 amino acids of the act-2 sequence (calculated with ATG methionine included). Sequence of deletion is (text inside of slashes is deleted, with 5' and 3' sequences shown): (exon#2)5'....gtgaaatcgtgcgtgacatc/aaggagaagctttgtt........ ...tggatagacattggtgt/gcgcactccttctggat.....3'(872 nucleotides from stop codon). Removes 489/1131 coding base pairs, beginning in second exn and extending beyond the 3' UTR. Mutagen: EMS Outcrossed: x>1 Made by: John Willis Received: 01/26/06 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1385 Species: Caenorhabditis elegans Genotype: dhc-1(or283ts) Description: Temperature-sensitive embryonic lethal. 100% dead embryos at 25 degrees. Maintain at 15 degrees. Small, posteriorly displaced first mitotic spindle with defective chomosome segregation and cytokinesis. Mutagen: EMS Outcrossed: x3 Made by: Sean O'Rourke Received: 07/13/09 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1386 Species: Caenorhabditis elegans Genotype: dhc-1(or352ts) Description: Temperature-sensitive embryonic lethal. 100% dead embryos at 25 degrees. Maintain at 15 degrees. Small, posteriorly displaced first mitotic spindle with defective chomosome segregation and cytokinesis. Mutagen: EMS Outcrossed: x3 Made by: Received: 07/13/09 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU139 Species: Caenorhabditis elegans Genotype: skn-1(or12) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Unc, WT which give only dead eggs, and dead eggs. Maintain by picking Unc. Received new stock 11/2002. Mutagen: EMS Outcrossed: x2 Made by: P Martin/B Bowerman Received: 11/27/01 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1404 Species: Caenorhabditis elegans Genotype: cyk-4(or749) III. Description: Temperature-sensitive Sterile and Embryonic lethal. Maintain at 15C. Homozygous or749 L4 larvae shifted to 25C become fertile adults that produce embryos with a late cytokinesis defect after the first embryonic mitosis. Reference: Canman JC, et al. Science. 2008 Dec 5;322(5907):1543-6. Mutagen: ENU Outcrossed: x4 Made by: Julie Canman Received: 08/20/13 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1424 Species: Caenorhabditis elegans Genotype: mom-2(or77) V/nT1 [unc-?(n754) let-?] (IV;V). Description: mom-2(or77) is a recessive, non-conditional maternal-effect embryonic lethal. Weak allele of mom-2/Wnt (splice acceptor mutation in last exon (exon6). 8% of mutant embryos lack gut. Heterozygotes are Unc. Mutagen: EMS Outcrossed: x2 Made by: Ann Schlesinger Received: 03/26/07 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU143 Species: Caenorhabditis elegans Genotype: skn-1(or13) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Unc, WT which give only dead eggs, and dead eggs. Maintain by picking Unc. Mutagen: EMS Outcrossed: x2 Made by: P Martin/B Bowerman Received: 11/27/01 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1441 Species: Caenorhabditis elegans Genotype: plk-1(or683) III. Description: Temperature sensitive embryonic lethal/sterile. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. Mutagen: ENU Outcrossed: x6 Made by: Bruce Bowerman Received: 04/11/11 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1444 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; orIs17. Description: orIs17 [dhc-1p::GFP::dhc-1 + unc-119(+)]. N-terminal-tagged GFP::dhc-1 fusion driven by the dhc-1 promoter. Reference: O'Rourke SM, et al. Nat Cell Biol. 2010 Dec;12(12):1235-41. Mutagen: Outcrossed: x1 Made by: Sean O'Rourke Received: 09/19/14 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1446 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; orIs14. Description: orIs14 [pie-1p::GFP::efa-6c::pie-1 3'UTR + unc-119(+)]. pie-1 promoter-driven N-terminal GFP fusion to EFA-6 isoform C. Reference: O'Rourke SM, et al. Nat Cell Biol. 2010 Dec;12(12):1235-41. Mutagen: Outcrossed: x0 Made by: Sean O'Rourke Received: 10/10/14 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1449 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; orIs19. Description: orIs19 [pie-1::GFP::dylt-1 + unc-119(+)]. Superficially wild-type. Reference: O'Rourke SM, et al. PLoS Genet. 2007 Aug;3(8):e128. Mutagen: Outcrossed: x0 Made by: Sean O'Rourke Received: 06/13/13 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1472 Species: Caenorhabditis elegans Genotype: let-99(or204) IV; him-5(e1490) V. Description: let-99(or204) is temperature-sensitive, maternal-effect, embryonic-lethal. Nearly completely penetrance of lethality at 26C. Viable at 15C. Maintain at 15C. Reference: Goulding MB, et al. J Cell Biol. 2007 Sep 24;178(7):1177-91. Mutagen: EMS Outcrossed: x5 Made by: Morgan Goulding Received: 04/06/11 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU148 Species: Caenorhabditis elegans Genotype: apx-1(or15) V/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Unc and wild type animals which give only dead progeny. Mutagen: Outcrossed: x Made by: Received: 10/18/02 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1481 Species: Caenorhabditis elegans Genotype: orIs20. Description: orIs20 [pie-1p::GFP::MOE]. Maintain under normal conditions. Mutagen: Outcrossed: x0 Made by: Sean O'Rourke Received: 04/15/13 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1485 Species: C. elegans Genotype: ltIs37 IV; orIs13. Description: ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. orIs13 [pie-1p::GFP::npp-22(genomic)::pie-1 3'UTR + unc-119(+)]. GFP-tagged NPP-22. Superficially wild-type. Might still carry unc-119(ed3) in background. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: O'Rourke SM, et al. PLoS Genet. 2007 Aug;3(8):e128. Mutagen: Outcrossed: x1 Made by: Sean O'Rourke Received: 05/31/19 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU149 Species: Caenorhabditis elegans Genotype: mex-3(or20)/hT1 I; +/hT1 V; lin-2(e1309) X. Description: Strain is egg-laying defective. Homozygous mex-3(or20) is a non-conditional maternal effect lethal: worms fill up with dead eggs. Heterozygotes bag with 11/16 dead embryos and larvae. About 10% of worms can lay eggs and be mated into for outcrossing. The mex-3(or20) mutant embryos produce excess body wall muscle anteriorly. Mutagen: EMS Outcrossed: x1 Made by: Chris Thorpe Received: 04/10/06 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1506 Species: Caenorhabditis elegans Genotype: dnc-4(or633) IV. Description: Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. Mutagen: EMS Outcrossed: x6 Made by: Clayton Carter Received: 11/15/11 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1510 Species: Caenorhabditis elegans Genotype: zyg-9(or634) II. Description: Temperature sensitive. Maintain at 15C. At 26C, animals give rise to 100% dead embryos, with embryos exhibiting lack of pronuclear migration at the one-cell stage. At 15C, animals give rise to viable progeny. Mutagen: EMS Outcrossed: x1 Made by: James Clayton Carter Received: 12/03/08 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: EU1513 Species: Caenorhabditis elegans Genotype: aph-1(or28)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III; lin-2(e1309) X. Description: or28 is a non-conditional maternal-effect embryonic lethal mutant ; the anterior pharynx is absent. and embryonic lethality is 100% penetrant. Pick single WT heterozygous hermaphrodites to maintain strain; these hets fill up with a mix of dead embryos and larvae [11/16 dead embryos due to aneuploidy from translocation segregation and lethal homozygous hT2; vulvaless due to homozygous lin-2(e1309) mutation; about 10% of lin-2(e1309) worms are leaky and lay eggs/can be mated into]. The homozygous or28 hermaphrodites fill up with dead embryos. The lin-2 background helps to score embryonic lethality for both heterozygotes and or28 homozygotes. or28 is a G to D mis-sense mutation at amino acid position 123. Mutagen: EMS Outcrossed: x1 Made by: Bruce Bowerman Received: 01/28/08 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1514 Species: Caenorhabditis elegans Genotype: aph-1(or28)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III; him-8(ec56) IV; lin-2(e1309) X. Description: or28 is a non-conditional maternal-effect embryonic lethal mutant ; the anterior pharynx is absent. and embryonic lethality is 100% penetrant. Pick single WT heterozygous hermaphrodites to maintain strain; these hets fill up with a mix of dead embryos and larvae [11/16 dead embryos due to aneuploidy from translocation segregation and lethal homozygous hT2; vulvaless due to homozygous lin-2(e1309) mutation; about 10% of lin-2(e1309) worms are leaky and lay eggs/can be mated into]. The homozygous or28 hermaphrodites fill up with dead embryos. The lin-2 background helps to score embryonic lethality for both heterozygotes and or28 homozygotes. Strain produces a lot of males due to him-8(ec56). or28 is a G to D mis-sense mutation at amino acid position 123. Mutagen: EMS Outcrossed: x1 Made by: Bruce Bowerman Received: 01/28/08 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1515 Species: Caenorhabditis elegans Genotype: aph-1(or28)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III; him-8(ec56) IV. Description: Him. or28 is a non-conditional maternal-effect embryonic lethal mutant ; the anterior pharynx is absent. and embryonic lethality is 100% penetrant. Pick single WT heterozygous hermaphrodites to maintain strain; these hets produce 11/16 dead embryos due to aneuploidy from translocation segregation and lethal homozygous hT2. The homozygous or28 hermaphrodites produce all dead embryos with defective pharyngeal development. or28 is a G to D mis-sense mutation at amino acid position 123. Mutagen: EMS Outcrossed: x1 Made by: Bruce Bowerman Received: 10/01/13 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1516 Species: Caenorhabditis elegans Genotype: aph-1(or28)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III. Description: or28 is a non-conditional maternal-effect embryonic lethal mutant ; the anterior pharynx is absent. and embryonic lethality is 100% penetrant. Pick single WT heterozygous hermaphrodites to maintain strain; these hets produce 11/16 dead embryos due to aneuploidy from translocation segregation and lethal homozygous hT2. The homozygous or28 hermaphrodites produce all dead embryos with defective pharyngeal development. or28 is a G to D mis-sense mutation at amino acid position 123. Strain produces lots of males due to him-8(ec56). Mutagen: EMS Outcrossed: x1 Made by: Bruce Bowerman Received: 10/01/13 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1588 Species: Caenorhabditis elegans Genotype: tbb-2(or600) III. Description: Semi-dominant. Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. Mutagen: ENU Outcrossed: x7 Made by: Luke Carter Received: 11/15/11 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1590 Species: Caenorhabditis elegans Genotype: zyg-12(or577) II. Description: Recessive, temperature-sensitive embryonic lethal mutant . Maintain at 15C, most embryos will hatch. Nearly all embryos fail to hatch at 25C. Centrosomes are detached from nuclear spindle envelope in one-cell stage embryos; sometimes reattach to pronuclei; sometimes stay unattached throughout prophase until nuclear envelope breakdown. Mis-sense mutation in Q367P. Hook family member. Localizes to nuclear envelope and centrosomes; interacts with SUN domain proteins at nuclear envelope and interacts with dynein. Mutagen: EMS Outcrossed: x6 Made by: Bruce Bowerman Received: 01/07/09 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1592 Species: Caenorhabditis elegans Genotype: aspm-1(or645) I. Description: Maintain at 15C; sterile at 26C. Shift to restrictive temperature (26C) for 3-6 hours to induce oocyte meiotic spindle phenotype and bypass sterility. Reference: Connolly A, et. al. Mol Biol Cell. 2014 Apr;25(8):1298-311. Mutagen: ENU Outcrossed: x6 Made by: Amy Connolly Received: 10/16/14 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU16 Species: Caenorhabditis elegans Genotype: dpy-18(e364) pie-1(zu154) III; skn-1(zu67)/nT1 [unc-?(n754) let-?] IV; +/nT1 V; eDp6 (III;f). Description: Heterozygotes are Unc. Segregates Uncs (dpy-18 pie-1; skn-1/DnT1; eDp6), Dpy non-Uncs (dpy-18 pie-1; skn-1 with no Dp) which give only dead embryos, and DpyUncs (dpy-18 pie-1; skn-1/DnT1 with no Dp) which give only dead eggs, WT looking (dpy-18 pie-1; skn-1; eDp6) which give only dead embryos, and dead eggs. Mutagen: Outcrossed: x Made by: Received: 08/03/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1600 Species: Caenorhabditis elegans Genotype: aspm-1(or645); ltIs37 IV; ojIs1. Description: ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. Temperature-sensitive. Viable at 15C. Complete loss of aspm-1 function at 26C. Shift to restrictive temperature for 3-6 hours to induce oocyte meiotic spindle phenotype and bypass sterility. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Connolly A, et al. Mol Biol Cell. 2014 Apr;25(8):1298-311. PMID: 24554763 Mutagen: ENU Outcrossed: x7 Made by: Amy Connolly Received: 11/11/14 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1617 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; orEx15. Description: orEx15 [pie-1p::GFP::rfl-1(genomic) + unc-119(+)]. Pick non-Unc to maintain. pie-1 promoter-driven N-terminal GFP fusion to RFL-1. Reference: Dorfman M, et al. Genetics. 2009 Aug;182(4):1035-49. Mutagen: Outcrossed: x0 Made by: Marc Dorfman Received: 03/27/14 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU1618 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; orEx16. Description: orEx16 [rfl-1p::tdTomato::rfl-1(genomic) + unc-119(+)]. Pick non-Unc to maintain. N-terminal tdTomato fusion to RFL-1 driven by rfl-1 promoter. Reference: Dorfman M, et al. Genetics. 2009 Aug;182(4):1035-49. Mutagen: Outcrossed: x0 Made by: Marc Dorfman Received: 03/27/14 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU176 Species: Caenorhabditis elegans Genotype: apx-1(or22) V/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Unc and wild type animals which give only dead progeny. Mutagen: Outcrossed: x Made by: Received: 10/18/02 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2005 Species: Caenorhabditis elegans Genotype: spd-2(or493) I. Description: Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. Mutagen: EMS Outcrossed: x1 Made by: Rebecca Luczak Received: 11/15/11 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2008 Species: Caenorhabditis elegans Genotype: zyg-1(or1018) II. Description: Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. Mutagen: ENU Outcrossed: x1 Made by: John Yochem Received: 01/20/12 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2009 Species: Caenorhabditis elegans Genotype: zyg-1(or409) II. Description: Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. Mutagen: EMS Outcrossed: x3 Made by: Minh Jones Received: 11/15/11 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2010 Species: Caenorhabditis elegans Genotype: spd-2(or454) I. Description: Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. Mutagen: EMS Outcrossed: x1 Made by: Jose-Eduardo Gomes Received: 11/15/11 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2470 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; orEx25. Description: orEx25 [GFP::aspm-1 + unc-119(+)]. Maintain at 25C. Pick non-Unc GFP+ to maintain. GFP might not be visible at low magnification. Reference: Connolly AA, et al. Mol Biol Cell. 2014 Apr;25(8):1298-311. Mutagen: Outcrossed: x1 Made by: Valerie Osterberg Received: 12/16/14 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2695 Species: Caenorhabditis elegans Genotype: klp-7(or1292) III; ltIs37 IV; ojIs1. Description: ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. Temperature-sensitive embryonic lethal. Maintain at 15C. Shift to 25-26C for 2-6 hours to induce meiotic spindle phenotype. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Connolly AA, et al. J Cell Biol. 2015 Sep 14;210(6):917-32. PMID: 26370499 Mutagen: Outcrossed: x2 Made by: Amy Connolly Received: 08/17/15 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2697 Species: Caenorhabditis elegans Genotype: mei-1(or1178) I; ItIs37 IV; ojIs1. Description: ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. Maintain at 15C; sterile at 26C. Shift to restrictive temperature (26C) for 3-6 hours to induce oocyte meiotic spindle phenotype and bypass sterility. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Connolly A, et. al. Mol Biol Cell. 2014 Apr;25(8):1298-311. Mutagen: ENU Outcrossed: x3 Made by: Amy Connolly Received: 10/16/14 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2706 Species: Caenorhabditis elegans Genotype: unc-119(ed3) ruIs32 III; drp-1(or1393) IV. Description: ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. Embryonic lethal at 26C. Maintain at 15C. Temperature-sensitive embryonic lethal following L4 upshift; germline defective following L1 upshift. Reference: Lowry J, et al. G3 (Bethesda). 2015 Aug 26;5(11):2241-55. Mutagen: ENU Outcrossed: x1 Made by: Josh Lowry Received: 04/22/16 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2711 Species: Caenorhabditis elegans Genotype: ndg-4(or1565) unc-119(ed3) ruIs32 III; ojIs1. Description: ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. Embryonic lethal at 26C. Maintain at 15C. Temperature-sensitive embryonic lethal following L4 upshift; germline defective following L1 upshift. Reference: Lowry J, et al. G3 (Bethesda). 2015 Aug 26;5(11):2241-55. Mutagen: ENU Outcrossed: x1 Made by: Josh Lowry Received: 06/01/16 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2715 Species: Caenorhabditis elegans Genotype: klp-7(or1092) III; ltIs37 IV; ojIs1. Description: ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. Temperature-sensitive embryonic lethal. Maintain at 15C. Shift to 25-26C for 2-6 hours to induce meiotic spindle phenotype. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Connolly AA, et al. J Cell Biol. 2015 Sep 14;210(6):917-32. PMID: 26370499 Mutagen: Outcrossed: x1 Made by: Amy Connolly Received: 09/17/15 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2836 Species: Caenorhabditis elegans Genotype: unc-32(e189) sas-7(or452) / qC1[dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregates WT, Sterile Dpys, and Unc. Unc worms produce temperature sensitive lethal embryos with centriole duplication defects. Keep at 15˚C to maintain or452 homozygotes. Ref. Sugioka, Hamill, et al., (2016) eLife 6 e20353. Mutagen: ENU Outcrossed: x6 Made by: Danielle Hamill Received: 08/18/17 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2858 Species: Caenorhabditis elegans Genotype: repo-1(or430) IV; itIs153; ojIs1. Description: itIs153 [pie-1p::par-2::GFP + rol-6(su1006) + N2 genomic DNA]. ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. Maintain at 15C. Temperature-sensitive embryonic-lethal at restrictive temperature of 26C; viable at permissive temperature of 15C. Reversed AP polarity axis at restrictive temperature with par-2::GFP and tubulin::GFP expression. itIs153 is an integrated derivitive of axEx1094. Reference: Keikhaee MR, et al. PLoS One. 2014 Sep 4;9(9):e106484. Mutagen: ENU Outcrossed: x5 Made by: MR Keikhaee Received: 10/10/14 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2861 Species: Caenorhabditis elegans Genotype: aspm-1(or1935[GFP::aspm-1]) I. Description: GFP::aspm-1 localizes to meiotic spindle poles and centrosomes during early development. Reference: Connolly AA, et al. J Cell Biol. 2015 Sep 14;210(6):917-32. PMID: 26370499 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Chien-Hui Chuang Received: 07/30/15 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2863 Species: Caenorhabditis elegans Genotype: klp-7(or1092) III. Description: Temperature-sensitive embryonic lethal. Maintain at 15C. Shift to 25-26C for 2-6 hours to induce meiotic spindle phenotype. Reference: Connolly AA, et al. J Cell Biol. 2015 Sep 14;210(6):917-32. PMID: 26370499 Mutagen: Outcrossed: x1 Made by: Amy Connolly Received: 09/17/15 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2866 Species: Caenorhabditis elegans Genotype: ltIs37 IV; orIs1. Description: ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. orIs1 [pie-1p::GFP::mei-1 + unc-119(+)]. Maintain at 25C to retain transgene fluorescence. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Connolly AA, et al. J Cell Biol. 2015 Sep 14;210(6):917-32. PMID: 26370499 Mutagen: Outcrossed: x1 Made by: Amy Connolly Received: 08/17/15 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2876 Species: Caenorhabditis elegans Genotype: aspm-1(or1935[GFP::aspm-1]) I; ltIs37 IV. Description: ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. GFP::aspm-1 localizes to meiotic spindle poles and centrosomes during early development. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Connolly AA, et al. J Cell Biol. 2015 Sep 14;210(6):917-32. PMID: 26370499 Mutagen: Outcrossed: x1 Made by: Amy Connolly Received: 07/30/15 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2882 Species: Caenorhabditis elegans Genotype: ndg-4(or1439) unc-119(ed3) ruIs32 III; ojIs1. Description: ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. Embryonic lethal at 26C. Maintain at 15C. Temperature-sensitive embryonic lethal following L4 upshift; germline defective following L1 upshift. Reference: Lowry J, et al. G3 (Bethesda). 2015 Aug 26;5(11):2241-55. Mutagen: ENU Outcrossed: x1 Made by: Josh Lowry Received: 06/01/16 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2883 Species: Caenorhabditis elegans Genotype: abtm-1(or891) I; unc-119(ed3) ruIs32 III; ojIs1. Description: ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. Embryonic lethal at 26C. Maintain at 15C. Temperature-sensitive embryonic lethal following L4 upshift; germline defective following L1 upshift. Reference: Lowry J, et al. G3 (Bethesda). 2015 Aug 26;5(11):2241-55. Mutagen: ENU Outcrossed: x1 Made by: Josh Lowry Received: 06/01/16 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2884 Species: Caenorhabditis elegans Genotype: atx-2(or821) unc-119(ed3) III; orIs21. Description: orIs21 [pie-1p::GFP::PH]. Embryonic lethal at 26C. Maintain at 15C. Temperature-sensitive embryonic lethal following L4 upshift; germline defective following L1 upshift. Reference: Lowry J, et al. G3 (Bethesda). 2015 Aug 26;5(11):2241-55. Mutagen: ENU Outcrossed: x1 Made by: Josh Lowry Received: 11/07/16 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2885 Species: Caenorhabditis elegans Genotype: abtm-1(or983) I; unc-119(ed3) ruIs32 III; ojIs1. Description: ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. Embryonic lethal at 26C. Maintain at 15C. Temperature-sensitive embryonic lethal following L4 upshift; germline defective following L1 upshift. Reference: Lowry J, et al. G3 (Bethesda). 2015 Aug 26;5(11):2241-55. Mutagen: ENU Outcrossed: x1 Made by: Josh Lowry Received: 04/22/16 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2887 Species: Caenorhabditis elegans Genotype: abtm-1(or1613) I; unc-119(ed3) ruIs32 III; ojIs1; orIs21. Description: ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. orIs21 [pie-1p::GFP::PH]. Embryonic lethal at 26C. Maintain at 15C. Temperature-sensitive embryonic lethal following L4 upshift; germline defective following L1 upshift. Reference: Lowry J, et al. G3 (Bethesda). 2015 Aug 26;5(11):2241-55. Mutagen: ENU Outcrossed: x1 Made by: Josh Lowry Received: 04/22/16 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2890 Species: Caenorhabditis elegans Genotype: abtm-1(or1400) I; unc-119(ed3) ruIs32 III. Description: ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. Embryonic lethal at 26C. Maintain at 15C. Temperature-sensitive embryonic lethal following L4 upshift; germline defective following L1 upshift. Reference: Lowry J, et al. G3 (Bethesda). 2015 Aug 26;5(11):2241-55. Mutagen: ENU Outcrossed: x1 Made by: Josh Lowry Received: 06/01/16 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2891 Species: Caenorhabditis elegans Genotype: abtm-1(or1184) I; unc-119(ed3) ruIs32 III; ojIs1; orIs21. Description: ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. orIs21 [pie-1p::GFP::PH]. Embryonic lethal at 26C. Maintain at 15C. Temperature-sensitive embryonic lethal following L4 upshift; germline defective following L1 upshift. Reference: Lowry J, et al. G3 (Bethesda). 2015 Aug 26;5(11):2241-55. Mutagen: ENU Outcrossed: x1 Made by: Josh Lowry Received: 04/22/16 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2892 Species: Caenorhabditis elegans Genotype: ndg-4(or1088) unc-119(ed3) ruIs32 III. Description: ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. Embryonic lethal at 26C. Maintain at 15C. Temperature-sensitive embryonic lethal following L4 upshift; germline defective following L1 upshift. Reference: Lowry J, et al. G3 (Bethesda). 2015 Aug 26;5(11):2241-55. Mutagen: ENU Outcrossed: x1 Made by: Josh Lowry Received: 04/22/16 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2893 Species: Caenorhabditis elegans Genotype: unc-119(ed3) ruIs32 III; drp-1(or1233) IV; ojIs1; orIs21. Description: ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. orIs21 [pie-1p::GFP::PH]. Embryonic lethal at 26C. Maintain at 15C. Temperature-sensitive embryonic lethal following L4 upshift; germline defective following L1 upshift. Reference: Lowry J, et al. G3 (Bethesda). 2015 Aug 26;5(11):2241-55. Mutagen: ENU Outcrossed: x1 Made by: Josh Lowry Received: 04/22/16 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2896 Species: Caenorhabditis elegans Genotype: rpl-7(or1247) I; unc-119(ed3) ruIs32 III. Description: ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. Embryonic lethal at 26C. Maintain at 15C. Temperature-sensitive embryonic lethal following L4 upshift; germline defective following L1 upshift. Reference: Lowry J, et al. G3 (Bethesda). 2015 Aug 26;5(11):2241-55. Mutagen: ENU Outcrossed: x1 Made by: Josh Lowry Received: 04/22/16 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2902 Species: Caenorhabditis elegans Genotype: rpl-7(or990) I; unc-119(ed3) ruIs32 III. Description: ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. Embryonic lethal at 26C. Maintain at 15C. Temperature-sensitive embryonic lethal following L4 upshift; germline defective following L1 upshift. Reference: Lowry J, et al. G3 (Bethesda). 2015 Aug 26;5(11):2241-55. Mutagen: ENU Outcrossed: x1 Made by: Josh Lowry Received: 04/22/16 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2906 Species: Caenorhabditis elegans Genotype: sqv-8(or1112) II; unc-119(ed3) ruIs32 III; ojIs1; orIs21. Description: ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. orIs21 [pie-1p::GFP::PH]. Embryonic lethal at 26C. Maintain at 15C. Temperature-sensitive embryonic lethal following L4 upshift; germline defective following L1 upshift. Reference: Lowry J, et al. G3 (Bethesda). 2015 Aug 26;5(11):2241-55. Mutagen: ENU Outcrossed: x1 Made by: Josh Lowry Received: 04/22/16 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2908 Species: Caenorhabditis elegans Genotype: ippk-1(or1572) II; unc-119(ed3) ruIs32 III. Description: ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. Embryonic lethal at 26C. Maintain at 15C. Temperature-sensitive embryonic lethal following L4 upshift; germline defective following L1 upshift. Reference: Lowry J, et al. G3 (Bethesda). 2015 Aug 26;5(11):2241-55. Mutagen: ENU Outcrossed: x1 Made by: Josh Lowry Received: 04/22/16 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2914 Species: Caenorhabditis elegans Genotype: sqv-8(or888) II; unc-119(ed3) ruIs32 III. Description: ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. Embryonic lethal at 26C. Maintain at 15C. Temperature-sensitive embryonic lethal following L4 upshift; germline defective following L1 upshift. Reference: Lowry J, et al. G3 (Bethesda). 2015 Aug 26;5(11):2241-55. Mutagen: ENU Outcrossed: x1 Made by: Josh Lowry Received: 04/22/16 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2917 Species: Caenorhabditis elegans Genotype: drp-1(or1941[drp-1::GFP]) IV. Description: Endogenous drp-1 locus tagged with GFP using CRISPR/Cas9. Reference: Lowry J, et al. G3 (Bethesda). 2015 Aug 26;5(11):2241-55. Mutagen: Outcrossed: x0 Made by: Chien-Hui Chuang Received: 03/22/17 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2934 Species: Caenorhabditis elegans Genotype: aspm-1(or1935[GFP::aspm-1]) I; klp-7(or1292) III; itIs37 IV. Description: itIs37 [pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)] IV. GFP::aspm-1 localizes to meiotic spindle poles and centrosomes during early development. Temperature-sensitive embryonic lethal. Maintain at 15C. Shift to 25-26C for 2-6 hours to induce meiotic spindle phenotype. Reference: Connolly AA, et al. J Cell Biol. 2015 Sep 14;210(6):917-32. PMID: 26370499 Mutagen: Outcrossed: x2 Made by: Amy Connolly Received: 07/30/15 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2936 Species: Caenorhabditis elegans Genotype: unc-69(e587) klp-7(or1092) III. Description: Temperature-sensitive embryonic lethal. Maintain at 15C. Shift to 25-26C for 2-6 hours to induce meiotic spindle phenotype. Reference: Connolly AA, et al. J Cell Biol. 2015 Sep 14;210(6):917-32. PMID: 26370499 Mutagen: Outcrossed: x1 Made by: Amy Connolly Received: 08/17/15 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2959 Species: Caenorhabditis elegans Genotype: vps-15(or1235ts) II; unc-119(ed3) III; orIs21. Description: orIs21 [pie-1p::GFP::PH]. Embryonic lethal at 26C. Maintain at 15C. Temperature-sensitive embryonic lethal following L4 upshift; germline defective following L1 upshift. Reference: Lowry J, et al. G3 (Bethesda). 2015 Aug 26;5(11):2241-55. Mutagen: ENU Outcrossed: x1 Made by: Josh Lowry Received: 11/07/16 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU2960 Species: Caenorhabditis elegans Genotype: crn-3(or959ts) II; ojIs1 IV. Description: ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)] IV. Embryonic lethal at 26C. Maintain at 15C. Temperature-sensitive embryonic lethal following L4 upshift; germline defective following L1 upshift. Reference: Lowry J, et al. G3 (Bethesda). 2015 Aug 26;5(11):2241-55. Mutagen: ENU Outcrossed: x1 Made by: Josh Lowry Received: 11/07/16 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU3000 Species: Caenorhabditis elegans Genotype: sas-7(or1940[gfp::sas-7]) III; ltIs37 IV. Description: sas-7(or1940[gfp::sas-7]) III. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Green centriole signal and red chromosome signal. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Sugioka, et al., 2017 Elife 6 e20353. Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Kenji Sugioka Received: 08/18/17 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU3020 Species: Caenorhabditis elegans Genotype: cls-2(or1948)/qC1[dpy-19(e1259) glp-1(q339) qIs26] III. Description: qIs26 [lag-2::GFP + rol-6(su1006)]. or1948 is a CRISPR/Cas9 engineered mutation in cls-1 causing a frameshift and premature stop. Heterozygotes are Rollers and GFP+ in the distal tip cell, and segregate heterozygotes (WT Rol), lethal qC1 homozygotes, and or1948 homozygotes (lay 100% dead embryos). Pick WT GFP+ Rol and check for correct segregation of progeny to maintain. Reference: Schlientz AJ & Bowerman B. (2020) C. elegans CLASP/CLS-2 negatively regulates membrane ingression throughout the oocyte cortex and is required for polar body extrusion. BioRxiv. Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Aleesa Schlientz Received: 09/01/20 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU3023 Species: Caenorhabditis elegans Genotype: cls-2(or1951)/qC1[dpy-19(e1259) glp-1(q339) qIs26] III. Description: qIs26 [lag-2::GFP + rol-6(su1006)]. or1951 is a CRISPR/Cas9 engineered mutation in cls-1 causing a frameshift and premature stop. Heterozygotes are Rollers and GFP+ in the distal tip cell, and segregate heterozygotes (WT Rol), lethal qC1 homozygotes, and or1951 homozygotes (lay 100% dead embryos). Pick WT GFP+ Rol and check for correct segregation of progeny to maintain. Reference: Schlientz AJ & Bowerman B. (2020) C. elegans CLASP/CLS-2 negatively regulates membrane ingression throughout the oocyte cortex and is required for polar body extrusion. BioRxiv. Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Aleesa Schlientz Received: 09/01/20 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU3030 Species: C elegans Genotype: ijmSi3 I; unc-119(ed3) III; ltIs37 IV. Description: ijmSi3 [mex-5p::cls-2(re-encoded)::GFP::tbb-2 3'UTR + Cbr-unc-119(+)] I. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. The coding sequence of GFP-tagged cls-2 in the transgene was re-coded using silent mutations to render it insensitive to RNAi-depletion of endogenous cls-2 expression. mCherry expression marks histones. Not known if unc-119(ed3) is still carried in the background of this strain. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Schlientz A and Bowerman B. PLoS Genet. 2020 Oct 7;16(10):e1008751. doi: 10.1371/journal.pgen.1008751. Mutagen: Outcrossed: x0 Made by: Aleesa Schlientz Received: 02/11/21 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU3068 Species: Caenorhabditis elegans Genotype: ebp-2(or1954[ebp-2::mKate2]) II; ruIs57. Description: ruIs57 [pie-1p::GFP::tubulin + unc-119(+)]. Superficially wild-type. mKate2 was inserted into the C-terminus of ebp-2 endogenous locus. Reference: Sugioka K, et al. (2018) PNAS, Jan 30;115(5): E954-E963. (PubMed ID: 29348204) Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Kenji Sugioka Received: 09/14/18 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU307 Species: Caenorhabditis elegans Genotype: +/szT1 [lon-2(e678)] I; mom-1(or46) dpy-6(e14)/szT1 X. Description: Heterozygotes are WT and segregate WT, Lon males, lots of dead eggs, and Dpys. The Dpys should give all dead embryos at all temperatures. Approximately 60% of these dead embryos make excess mesoderm at the expense of endoderm, due to an E to MS fate transformation. Embryonic lethality is 100% penetrant for or46. Mutagen: EMS Outcrossed: x3 Made by: Chris Thorpe Received: 07/31/06 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU308 Species: Caenorhabditis elegans Genotype: +/szT1 [lon-2(e678)] I; mom-1(or10) dpy-6(e14)/szT1 X. Description: Heterozygotes are WT and segregate WT, Lon males, lots of dead eggs, and Dpys. The Dpys should give all dead embryos at all temperatures. Approximately 60% of these dead embryos make excess mesoderm at the expense of endoderm, due to an E to MS fate transformation. Embryonic lethality is 100% penetrant for or10. Mutagen: EMS Outcrossed: x3 Made by: Chris Thorpe Received: 07/31/06 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU3095 Species: C elegans Genotype: aspm-1(or1935[GFP::aspm-1]) I; zyg-9(or1984) II; ltIs37 IV. Description: ItIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Maintain at 15C. Fast-acting temperature-sensitive allele. At restrictive temperature (26C), animals give rise to 100% dead embryos. At 15C, animals give rise to viable progeny. GFP::aspm-1 localizes to meiotic spindle poles and centrosomes during early development. Might still carry unc-119(ed3) III in background. Reference: Harvey AM, et al. PLoS Genet. 2023 Jan 6;19(1):e1010363. doi: 10.1371/journal.pgen.1010363. PMID: 36608115 Mutagen: ENU Outcrossed: x2 Made by: Chien-Hui Chuang Received: 02/16/23 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU31 Species: Caenorhabditis elegans Genotype: skn-1(zu135) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Unc, WT which give only dead eggs, and dead eggs. Maintain by picking Unc. Mutagen: EMS Outcrossed: x3 Made by: P Martin/B Bowerman Received: 11/27/01 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU311 Species: Caenorhabditis elegans Genotype: dpy-5(e61) mom-5(or57)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III. Description: Heterozygotes are WT and segregate WT, Dpys and dead eggs. Dpys give only embryonic lethals which lack endoderm and make excess mesoderm. or57 is a recessive maternal-effect lethal. Mutagen: mut-2 Outcrossed: x4 Made by: Chris Thorpe Received: 12/15/97 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU3115 Species: C elegans Genotype: klp-15(ok1958) klp-16(or1952)/tmC18[dpy-5(tmIs1236)] I; ltIs37 IV; ruIs57. Description: ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. ruIs57 [pie-1p::GFP::tubulin + unc-119(+)]. tmC18 balancer marked with myo-2p::mCherry and Dpy. Heterozygotes are wild-type with pharyngeal mCherry, and segregate mCherry+ heterozygotes, tmC18 homozygotes (mCherry+ Dpy) and non-mCherry klp-15/16 homozygotes. Homozygous double deletion mutants are fertile but produced reduced brood sizes with highly penetrant embryonic lethality; will also segregate some males. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Chuang CH, et al., Biology Open 2020 9: bio052308 doi: 10.1242/bio.052308 Published 25 June 2020 Mutagen: Outcrossed: x1 Made by: Jie Yang Received: 09/01/20 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU3121 Species: C. elegans Genotype: tac-1(or1955[gfp::tac-1]) II; ltIs37 IV. Description: ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. GFP tag inserted into endogenous tac-1 locus. Might still contain unc-119(ed3) in the background. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Chuang CH, et al., Biology Open 2020 9: bio052308 doi: 10.1242/bio.052308 Published 25 June 2020 Mutagen: Outcrossed: x1 Made by: Chien-Hui Chuang Received: 09/01/20 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU3169 Species: C. elegans Genotype: zyg-9(or1956[gfp::zyg-9]) II; ltIs37 IV. Description: ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. GFP tag inserted into endogenous zyg-9 locus. Might still contain unc-119(ed3) in the background. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Chuang CH, et al., Biology Open 2020 9: bio052308 doi: 10.1242/bio.052308 Published 25 June 2020 Mutagen: Outcrossed: x1 Made by: Chien-Hui Chuang Received: 09/01/20 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU3201 Species: C elegans Genotype: klp-15(ok1958) aspm-1(syb1260[gfp::aspm-1]) klp-16(or1952) /tmC18[dpy-5(tmIs1236)] I; ltIs37[pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)] IV Description: ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. ruIs57 [pie-1p::GFP::tubulin + unc-119(+)]. GFP tag inserted into endogenous aspm-1 locus. tmC18 balancer marked with myo-2p::mCherry and Dpy. Heterozygotes are wild-type with pharyngeal mCherry, and segregate mCherry+ heterozygotes, tmC18 homozygotes (mCherry+ Dpy) and non-mCherry triple mutant homozygotes. Homozygous triple mutants are fertile but produced reduced brood sizes with highly penetrant embryonic lethality; will also segregate some males. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Chuang CH, et al., Biology Open 2020 9: bio052308 doi: 10.1242/bio.052308 Published 25 June 2020 Mutagen: Outcrossed: x1 Made by: Jie Yang Received: 09/01/20 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU3206 Species: C elegans Genotype: sas-6(or1167) IV. Description: Temperature-sensitive embryonic lethal. Maintain at 15C with 20% to 50% hatching rate. 100% lethality and mono-polar spindle at 26C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. Mutagen: Outcrossed: x2 Made by: Chien-Hui Chuang Received: 11/19/19 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU3407 Species: C elegans Genotype: zyg-9(or1985)/mnC1[dpy-10(e128) unc-52(e444) umnIs32] II. Description: umnIs32 [myo-2p::GFP + NeoR, II: 11755713 (intergenic)] II. or1985 is a CRISPR/Cas9 engineered deletion of zyg-9 removing the entire open reading frame. Heterozygotes are wild-type and GFP+ and segregate WT GFP+ (hets), or1948 homozygotes (GFP-, lay 100% dead embryos) and paralysed DpyUnc GFP+ (mnC1 homozygotes). Maintain by picking WT GFP+. Reference: Harvey AM, et al. PLoS Genet. 2023 Jan 6;19(1):e1010363. doi: 10.1371/journal.pgen.1010363. PMID: 36608115 Mutagen: Outcrossed: x1 Made by: Chien-Hui Chuang Received: 02/16/23 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU35 Species: Caenorhabditis elegans Genotype: skn-1(zu169) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Unc, WT which give only dead eggs, and dead eggs. Maintain by picking Unc. Mutagen: mut-7 Outcrossed: x2 Made by: P Martin/B Bowerman Received: 11/27/01 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU361 Species: Caenorhabditis elegans Genotype: +/szT1 [lon-2(e678)] I; mom-1(or10) unc-6(n102)/szT1 X. Description: Heterozygotes are WT and segregate WT, Uncs with a protruding vulva, Long males and dead eggs. Uncs give only embryonic lethals which lack gut and make excess mesoderm (E adopts an MS-like fate). or10 is a recessive maternal-effect embryonic lethal. Zygotic phenotype of or10 is protruding vulva and slight uncoordination. Mutagen: EMS Outcrossed: x6 Made by: Bruce Bowerman Received: 12/15/97 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU363 Species: Caenorhabditis elegans Genotype: let-99(or81) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc. Mutagen: Outcrossed: x Made by: Received: 05/07/03 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU365 Species: Caenorhabditis elegans Genotype: mom-2(or85) V/nT1 [unc-?(n754) let-?] (IV;V). Description: mom-2(or85) is a recessive, non-conditional maternal-effect embryonic lethal. Strong allele; missense mutation near N-terminus: L77P. 85% of mutant embryos lack gut. Heterozygotes are Unc. [NOTE: (08-27-2014) We have been informed that the mutation carried in this strain is the same molecular lesion as or77. We are working to determine if or85 and or77 are in fact the same lesion or if an incorrect genotype was provided for the strain.] Mutagen: EMS Outcrossed: x2 Made by: Ann Schlesinger Received: 03/12/07 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU367 Species: Caenorhabditis elegans Genotype: glp-1(e2141) III; mom-2(or42) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Maintain at 15C. Animals are Unc. Mutagen: Outcrossed: x Made by: Received: 03/06/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU370 Species: Caenorhabditis elegans Genotype: spn-4(or25) V/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and lay 10/16 dead embryos. spn-4 homozygotes look WT but are non-conditional maternal effect embryonic lethals. Embryos from homozygous mothers exhibit defects in mitotic spindle orientation and cell fate patterning. Mutagen: EMS Outcrossed: x4 Made by: C. Shelton Received: 02/14/02 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU384 Species: Caenorhabditis elegans Genotype: dpy-11(e1180) mom-2(or42) V/nT1 [let-?(m435)] (IV;V). Description: Heterozygotes are WT and segregate WT, Dpys and dead eggs. Dpys segregate only embryonic lethals which lack endoderm and make excess mesoderm with E adopting and MS-like fate. or42 is a recessive maternal-effect lethal. Mutagen: EMS Outcrossed: x6 Made by: Chris Thorpe Received: 12/15/97 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU396 Species: Caenorhabditis elegans Genotype: cyk-1(or36) unc-36(e251)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, Sterile Dpys and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x Made by: Swan and Bowerman Received: 04/05/99 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU40 Species: Caenorhabditis elegans Genotype: skn-1(zu129) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Unc, WT which give only dead eggs, and dead eggs. Maintain by picking Unc. Mutagen: EMS Outcrossed: x3 Made by: P Martin/B Bowerman Received: 11/27/01 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU404 Species: Caenorhabditis elegans Genotype: rol-1(e91) mig-14(or78)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, Uncs and Rollers. or78 zygotic effects: Pv, mild Unc, many explode at vulva upon reaching adulthood. or78 maternal effects: endoderm (E) transformed into mesoderm (MS) in early embryo, severe morphogenesis defect. mig-14, mom-3, pvl-2, and let-553 are the same gene. Mutagen: EMS Outcrossed: x7 Made by: Ann Schlesinger Received: 02/17/98 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU407 Species: Caenorhabditis elegans Genotype: mom-4(or39)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III; him-8(e1489) IV; lin-2(e1309) X. Description: Mutagen: Outcrossed: x Made by: Received: 03/30/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU41 Species: Caenorhabditis elegans Genotype: skn-1(or19) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Unc, WT which give only dead eggs, and dead eggs. Maintain by picking Unc. Mutagen: EMS Outcrossed: x2 Made by: P Martin/B Bowerman Received: 11/27/01 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU414 Species: Caenorhabditis elegans Genotype: unc-13(e1091) mom-4(or39)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III. Description: Heterozygotes are WT. Segregates Uncs which give only embryonic lethals which lack gut and make excess mesoderm (E adopts an MS-like fate). or39 is a recessive maternal-effect embryonic lethal. Mutagen: EMS Outcrossed: x6 Made by: Bruce Bowerman Received: 12/15/97 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU415 Species: Caenorhabditis elegans Genotype: cyk-1(or36) unc-36(e251)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-8(e1489) IV. Description: Heterozygotes are WT and segregate WT, Sterile Dpys and Uncs which give only dead eggs at all temperatures. Throws males. Mutagen: Outcrossed: x Made by: B Bowerman Received: 07/24/08 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU417 Species: Caenorhabditis elegans Genotype: mom-2(or33) V/nT1 [unc-?(n754) let-?] (IV;V). Description: mom-2(or33) is a recessive, non-conditional maternal-effect embryonic lethal. Intermediate strength allele; missense mutation near C-terminus: C321Y. 50% of mutant embryos lack gut. Heterozygotes are Unc. Mutagen: EMS Outcrossed: x2 Made by: Chris Thorpe Received: 03/12/07 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU423 Species: Caenorhabditis elegans Genotype: dpy-5(e61) mom-4(or39)/hT1 I; mom-2(or42) unc-42(e270)/hT1 V. Description: Heterozygotes are WT. Mutagen: Outcrossed: x Made by: Received: 05/07/03 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU443 Species: Caenorhabditis elegans Genotype: unc-13(e1091) mom-4(or11)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III. Description: Heterozygotes are WT. Segregates Uncs which give only embryonic lethals which lack gut and make excess mesoderm (E adopts an MS-like fate). or11 is a recessive maternal-effect embryonic lethal. Mutagen: EMS Outcrossed: x Made by: Received: 08/03/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU446 Species: Caenorhabditis elegans Genotype: unc-13(e1091) mom-4(or39)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III. Description: Heterozygotes are WT. Segregates Uncs which give only embryonic lethals which lack gut and make excess mesoderm (E adopts an MS-like fate). or39 is a recessive maternal-effect embryonic lethal. Mutagen: EMS Outcrossed: x Made by: Received: 08/03/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU447 Species: Caenorhabditis elegans Genotype: unc-13(e1091) mom-4(or39)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III; him-8(e1489) IV. Description: Heterozygotes are WT. Segregates Uncs which give only embryonic lethals which lack gut and make excess mesoderm (E adopts an MS-like fate). or39 is a recessive maternal-effect embryonic lethal. Throws males. Mutagen: EMS Outcrossed: x Made by: Received: 08/03/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU452 Species: Caenorhabditis elegans Genotype: mom-5(zu193) unc-13(e1091)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III. Description: Heterozgyotes are WT and segregate WT, Uncs which give only dead eggs, and dead eggs. Mutagen: Outcrossed: x Made by: Received: 08/21/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU459 Species: Caenorhabditis elegans Genotype: unc-13(e1091) mom-5(zu193)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III; him-8(ec56) IV. Description: Heterozygotes are WT and segregate WT and Uncs. The Uncs have a non-conditional maternal-effect embryonic lethal phenotype: the E blastomere adopts MS fate in about 5% of mutant embryos. The Uncs are 100% penetrant for embyronic lethality and morphogenesis defects. Mutagen: Tc1 Outcrossed: x4 Made by: Craig Mello Received: 01/28/08 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU548 Species: Caenorhabditis elegans Genotype: div-1(or148) III. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x2 Made by: Received: 12/15/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU550 Species: Caenorhabditis elegans Genotype: div-1(or148) III; him-8(e1489) IV. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x2 Made by: Received: 12/15/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU552 Species: Caenorhabditis elegans Genotype: glp-1(or178) III. Description: Temperature sensitive. At 25C: Ste, during embryogenesis. Strong embryonic phenotype (anterior pharynx missing, no morophogenesis) if shifted during adulthood. Mutagen: EMS Outcrossed: x2 Made by: Danielle Hamill Received: 03/05/98 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU554 Species: Caenorhabditis elegans Genotype: zen-4(or153) IV. Description: Temperature sensitive. Grow at permissive temperature of 15C. Fully penetrant cytokinesis defect at the restrictive temperature of 25C. Mutagen: EMS Outcrossed: x Made by: DR Hamill Received: 11/04/02 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU558 Species: Caenorhabditis elegans Genotype: spd-2(or183) I. Description: Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. Mutagen: EMS Outcrossed: x1 Made by: Rebecca Luczak Received: 01/20/12 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU573 Species: Caenorhabditis elegans Genotype: orEx2. Description: orEx2 [mlc-4p::mlc-4(genomic coding)::GFP::unc-54 3'UTR + rol-6(su1006)]. Maintain by picking Rollers. Rollers are GFP+. Mutagen: Outcrossed: x Made by: C. Shelton Received: 09/22/06 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU588 Species: Caenorhabditis elegans Genotype: him-8(e1489) IV; spn-4(or191) V. Description: Temperature sensitive. Viable at 15C; lethal at 25C. At 25C, embryos exhibit defects in spindle orientation and cell fate patterning. Throws males. Mutagen: EMS Outcrossed: x5 Made by: J-E. Gomes Received: 02/14/02 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU591 Species: Caenorhabditis elegans Genotype: dpy-13(e184) zen-4(or153) IV. Description: Temperature sensitive. Grow at permissive temperature of 15C. Fully penetrant cytokinesis defect at the restrictive temperature of 25C. Semi-dominant Dpy. Mutagen: Outcrossed: x Made by: DR Hamill Received: 11/04/02 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU592 Species: Caenorhabditis elegans Genotype: unc-8(n491) zen-4(or153) IV. Description: Temperature sensitive. Grow at permissive temperature of 15C. Fully penetrant cytokinesis defect at the restrictive temperature of 25C. Semi-dominant Unc. Mutagen: Outcrossed: x Made by: DR Hamill Received: 11/04/02 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU593 Species: Caenorhabditis elegans Genotype: mel-26(or184) I. Description: Temperature sensitive. Maintain at 15C. At 25C, hermaphrodites lay embryos that die. Transverse PO mitotic spindle, cytokinesis defects. Missense mutation at nucleotide #245 (gga->gaa); amino acid #82 (G to E). Mutagen: EMS Outcrossed: x2 Made by: Danielle Hamill Received: 01/13/04 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU603 Species: Caenorhabditis elegans Genotype: lit-1(or131) III; him-8(e1489) IV. Description: or131 mutant embyros have a highly penetrant mom-like defect with mutant embryos lacking gut and making extra pharyngeal tissue at 25C. Maintain at 15C. Mutagen: Outcrossed: x Made by: Danielle Hamill Received: 08/03/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU618 Species: Caenorhabditis elegans Genotype: mlc-4(or253)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, DpySteriles and late embryonic or early larval lethals with incomplete elongation/morphogenesis. Mutagen: EMS Outcrossed: x3 Made by: Chris Shelton Received: 01/20/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU626 Species: Caenorhabditis elegans Genotype: rfl-1(or198) III. Description: Temperature sensitive maternal effect lethal mutation in F11H8.1 (Nedd8 activating enzyme). Permissive temperature is 15C, restrictive temperature is 25C. Embryos layed at restrictive temperature have spindle-orientation defects due to the mis-localization of MEI-1/MEI-2 to the mitotic spindle. Additionally, ectopic cleavage furrows are initiated during cytokinesis, and cell-cycle delays are apparent during interphase. Mutagen: EMS Outcrossed: x6 Made by: Danielle Hamill Received: 04/29/02 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU630 Species: Caenorhabditis elegans Genotype: air-2(or207) I. Description: Temperature sensitive. Grow at permissive temperature of 15C. Fully penetrant cytokinesis defect at the restrictive temperature of 25C. Mutagen: EMS Outcrossed: x Made by: DR Hamill Received: 11/04/02 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU640 Species: Caenorhabditis elegans Genotype: cul-2(or209) III. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x1 Made by: Received: 12/15/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU641 Species: Caenorhabditis elegans Genotype: cul-2(or209) III; him-8(e1489) IV. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x1 Made by: Received: 12/15/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU704 Species: Caenorhabditis elegans Genotype: zyg-1(or278) II. Description: Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. Mutagen: EMS Outcrossed: x1 Made by: Minh Jones Received: 11/15/11 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU707 Species: Caenorhabditis elegans Genotype: air-2(or207) unc-13(e51) I. Description: Temperature sensitive. Grow at permissive temperature of 15C. Fully penetrant cytokinesis defect at the restrictive temperature of 25C. Semi-dominant Unc. Mutagen: Outcrossed: x Made by: DR Hamill Received: 11/04/02 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU716 Species: Caenorhabditis elegans Genotype: zen-4(or153) IV. Description: Temperature-sensitive, embryonic-lethal mutant that lacks a central spindle during early embryonic mitosis and exhibits a late cytokinesis defect (cleavage furrows regress after ingressing in nearly to the center of dividing embryonic cells). About 100% of embryos produced by homozygous mothers hatch at 15C; 0% hatch at 26C. ZEN-4 = vertebrate MKLP1 kinesin. There are two mis-sense mutations present in zen-4(or153). One is a D520N (GAC to AAC) and the other is D735N (GAT to AAT). Whether one or both is responsible for the phenotype is not know. Maintain at 15C. Shift L4s to 26 overnight to observe mutant phenotype on embyros produced by adults. Mutagen: EMS Outcrossed: x3 Made by: Aaron Severson Received: 03/16/06 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU718 Species: Caenorhabditis elegans Genotype: div-2(or182) III. Description: Temperature sensitive. Maintain at 15C. CGC received new stock 2/2002. Mutagen: Outcrossed: x3 Made by: Received: 12/15/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU769 Species: Caenorhabditis elegans Genotype: spn-4(or25) unc-42(e270) V/nT1 [let-?(m435)] (IV;V). Description: Heterozygotes are WT and lay 10/16 dead eggs. spn-4 unc-42 homozygotes are Unc and lay dead embryos exhibiting defects in mitotic spindle orientation and cell fate patterning. spn-4(or25) homozygotes are non-conditional maternal effect embryonic lethal. Mutagen: EMS Outcrossed: x4 Made by: C. Shelton Received: 02/14/02 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU772 Species: Caenorhabditis elegans Genotype: spn-4(or80) V/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and lay 10/16 dead embryos. spn-4 homozygotes look WT but are non-conditional maternal effect embryonic lethals. Embryos from homozygous mothers exhibit defects in mitotic spindle orientation and cell fate patterning. Mutagen: EMS Outcrossed: x3 Made by: J-E. Gomes Received: 02/14/02 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU779 Species: Caenorhabditis elegans Genotype: mex-1(or286) II. Description: Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. Mutagen: EMS Outcrossed: x2 Made by: Rebecca Luczak Received: 11/15/11 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU780 Species: Caenorhabditis elegans Genotype: spd-2(or293) I; him-8(e1489) IV. Description: Him. Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. Mutagen: EMS Outcrossed: x1 Made by: Rebecca Luczak Received: 11/15/11 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU782 Species: Caenorhabditis elegans Genotype: zyg-1(or297) II. Description: Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. Mutagen: EMS Outcrossed: x1 Made by: Minh Jones Received: 04/10/12 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU822 Species: Caenorhabditis elegans Genotype: par-2(or373) III; lin-2(e1309) X. Description: Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. Mutagen: EMS Outcrossed: x0 Made by: Rebecca Luczak Received: 04/10/12 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU828 Species: Caenorhabditis elegans Genotype: dhc-1(or195) I. Description: Homozygous or195 hermaphrodites make 100% dead embryos at 26C. One-cell embryo has a very small but bipolar spindle, severe chromosome segregation defect. Maintain at 15C. Previously called spd-4. 2/06: From Bruce Bowerman: The or195 mutation changes base 10,679 of the cosmid clone T21E12 from a C to a T. This corresponds to dhc-1 cDNA nucleotide 9599 (WS153) , which is the center nucleotide of codon 3200. This codon changes from Serine in N2 to Leucine in or195 (S3200L). Note: this is not the same mutation for or195ts that we reported in Hamill et al, 2002(Dev Cell 3, 673-684). The mutation reported in our paper is not present in this strain. We apologize for the confusion. The pairs dhc-11a,b amplify the mutated fragment in dhc-1(or195). The mutation is near the center of this fragment; a clean (gel purified) DNA prep helps get a good read of it. dhc-11b: 5' aacagacgcacgattgacct 3'. dhc-11a: 5' ctcaaatcaaggaaggagct 3'. PCR conditions: 5 min at 94 degrees C; 30 sec at 94 degrees C; 30 sec at 55 degrees C; 1 min at 72 degrees C. 35 Cycles using Taq polymerase. Mutagen: EMS Outcrossed: x4 Made by: Danielle Hamill Received: 01/27/03 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU833 Species: Caenorhabditis elegans Genotype: div-2(or234) III. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x Made by: Received: 02/13/02 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU84 Species: Caenorhabditis elegans Genotype: unc-5(e53) skn-1(zu67) IV/nT1 [let-?(m435)] (IV;V). Description: Heterozygotes are WT and segregate WT, Uncs which give only dead eggs, and dead eggs. Mutagen: Outcrossed: x Made by: Received: 10/02/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU855 Species: Caenorhabditis elegans Genotype: mom-2(or309) V/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Uncs, WT which give only dead eggs, and dead eggs. or309 is a deletion allele: removes exon 4 to 3' end and leave about 100 N-terminal amino acids. Mutagen: Outcrossed: x Made by: Received: 08/21/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU856 Species: Caenorhabditis elegans Genotype: spd-5(or213) I. Description: Homozygous or213 hermaphrodites make 100% dead embryos at 26C. No mitotic spindle assembly. Maintain at 15C. Mutagen: EMS Outcrossed: x4 Made by: Danielle Hamill Received: 01/27/03 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU858 Species: Caenorhabditis elegans Genotype: tbb-2(or362) III. Description: Conditional, semi-dominant, maternal-effect, embryonic-lethal mutation. Once-cell stage mitotic embryos have short astral microtubules and a mispositioned mitotic spindle. Maintain at 15C. Mutagen: EMS Outcrossed: x4 Made by: Bruce Bowerman Received: 05/06/04 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU863 Species: Caenorhabditis elegans Genotype: div-(or361) him-8(e1489) IV. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x1 Made by: Received: 12/15/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU867 Species: Caenorhabditis elegans Genotype: div-(or389) II. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x1 Made by: Received: 12/15/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU868 Species: Caenorhabditis elegans Genotype: div-(or361) IV. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x1 Made by: Received: 12/15/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU869 Species: Caenorhabditis elegans Genotype: div-(or389) II; him-8(e1489) IV. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x1 Made by: Received: 12/15/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU871 Species: Caenorhabditis elegans Genotype: div-(or235) III; him-8(e1489) IV. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x1 Made by: Received: 12/15/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU872 Species: Caenorhabditis elegans Genotype: div-(or366) I; him-8(e1489) IV. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x1 Made by: Received: 12/15/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU873 Species: Caenorhabditis elegans Genotype: div-(or310) I. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x1 Made by: Received: 12/15/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU878 Species: Caenorhabditis elegans Genotype: div-(or335) I; him-8(e1489) IV. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x1 Made by: Received: 12/15/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU879 Species: Caenorhabditis elegans Genotype: div-1(or345) III; him-8(e1489) IV. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x1 Made by: Received: 12/15/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU880 Species: Caenorhabditis elegans Genotype: div-1(or345) III. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x1 Made by: Received: 12/15/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU882 Species: Caenorhabditis elegans Genotype: div-(or310) I; him-8(e1489) IV. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x1 Made by: Received: 12/15/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU883 Species: Caenorhabditis elegans Genotype: div-(or364) III; him-8(e1489) IV. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x1 Made by: Received: 12/15/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU888 Species: Caenorhabditis elegans Genotype: div-(or366) I. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x1 Made by: Received: 12/15/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU91 Species: Caenorhabditis elegans Genotype: apx-1(or3) V/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Unc and wild type animals which give only dead progeny. Mutagen: Outcrossed: x Made by: Received: 10/18/02 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU918 Species: Caenorhabditis elegans Genotype: lon-1(e185) par-3(it71)/qC1 [dpy-19(e1259) glp-1(q339)] III; spn-4(or191) V. Description: Temperature sensitive spn-1. Maintain at 15C. Lethal at 25C. Heterozygotes are WT and segregate WT, Lon which give only dead eggs at all temperatures, and Sterile Dpys(ts). Mutagen: Outcrossed: x5 Made by: J-E. Gomes Received: 02/14/02 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU920 Species: Caenorhabditis elegans Genotype: lit-1(or393) III; him-8(e1489) IV. Description: Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. Mutagen: EMS Outcrossed: x2 Made by: Rebecca Luczak Received: 11/15/11 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU923 Species: Caenorhabditis elegans Genotype: spn-4(or191) V. Description: Temperature sensitive. Viable at 15C; lethal at 25C. At 25C, embryos exhibit defects in spindle orientation and cell fate patterning. Mutagen: EMS Outcrossed: x5 Made by: J-E. Gomes Received: 02/14/02 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU924 Species: Caenorhabditis elegans Genotype: zyg-8(or484) III; lin-2(e1309) X. Description: Temperature-sensitive. Maintain at 15C. Reference: Gonczy P, et al. Dev Cell. 2001 Sep;1(3):363-75. Mutagen: ENU Outcrossed: x0 Made by: Sean O'Rourke Received: 06/13/13 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU927 Species: Caenorhabditis elegans Genotype: dpy-5(e61) mom-4(or49)/hT2 I; +/hT2 [bli-4(e937) let-?(h661)] III. Description: Heterozygotes are WT and segregate WT, Dpys which give only dead eggs and dead eggs. Mutagen: Outcrossed: x Made by: Received: 08/21/00 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU931 Species: Caenorhabditis elegans Genotype: zyg-8(or490) III; lin-2(e1309) X. Description: Maintain at 15C. Temperature-sensitive embryonic-lethal at restrictive temperature of 26C. Viable embryos at permissive temperature of 15C. Mitotic spindle in one-cell stage embryo assembles normally, but microtubules destabilize at anaphase and spindle becomes mis-positioned toward the posterior pole leading to highly abnormal cleavage and chromosome segregation defects. References: Gonczy P, et al. Dev Cell. 2001 Sep;1(3):363-75. Bellanger JM, et al. J Cell Sci. 2007 Aug 15;120(Pt 16):2963-73. Mutagen: ENU Outcrossed: x0 Made by: Jennifer B. Phillips Received: 10/31/13 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EU944 Species: Caenorhabditis elegans Genotype: tac-1(or455) II. Description: Homozygous mutant animals give rise to >99% dead embyros at the restrictive temperature of 26C, with embryos exhibiting a lack of pronuclear migration at the one-cell stage. Homozygous mutant animals give rise to viable progeny at the permissive temperature of 15C. Maintain at 15C. Mutagen: EMS Outcrossed: x1 Made by: Jennifer Phillips Received: 05/15/08 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: EU948 Species: Caenorhabditis elegans Genotype: par-2(or539) III. Description: Temperature-sensitive embryonic lethal. Maintain at 15C. Reference: O'Rourke SM, et al. PLoS One. 2011 Mar 1;6(3):e16644. Mutagen: EMS Outcrossed: x1 Made by: Rebecca Luczak Received: 04/10/12 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: EV190 Species: Caenorhabditis elegans Genotype: gld-4(ef15) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Homozygous nearly sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ef15 homozygotes (pale, nearly sterile, lays few eggs). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Schmid M, et al. Genes Dev. 2009 Apr 1;23(7):824-36. Mutagen: EMS Outcrossed: x10 Made by: Mark Schmid Received: 06/01/11 Martin Luther University, Halle-Wittenberg, Halle, Germany ----------------------------------------------------------------------------- Strain: EV232 Species: Caenorhabditis elegans Genotype: gck-3(tm1296)/dpy-21(e428) V. Description: Heterozygotes are wild-type and segregate wild-type (heterozygotes), Dpy, and sterile tm1296 homozygotes. Dev Biol. 2010 Aug 15;344(2):758-71. Mutagen: TMP/UV Outcrossed: x10 Made by: Adam Kupinski Received: 06/01/11 Martin Luther University, Halle-Wittenberg, Halle, Germany ----------------------------------------------------------------------------- Strain: EV247 Species: Caenorhabditis elegans Genotype: nhr-114(ef24) V. Description: Reference: Gracida X, Eckmann CR. Curr Biol. 2013 Apr 8;23(7):607-13. Mutagen: EMS Outcrossed: x11 Made by: Xico Gracida Received: 04/22/13 Martin Luther University, Halle-Wittenberg, Halle, Germany ----------------------------------------------------------------------------- Strain: EV343 Species: Caenorhabditis elegans Genotype: unc-119(ed3); efEx12. Description: efEx12 [glp-1::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. Pick non-Unc to maintain. GFP expression in the proliferative region of the germ line (resembling endogenous GLP-1 protein localization), and also in spermatheca and other somatic tissues. Derived by bombarding strain DP38 with LAP-tagged glp-1 fosmid (WRM0630DF02). Mutagen: Bombardment Outcrossed: x0 Made by: Sophia Millonigg Received: 03/02/17 Martin Luther University, Halle-Wittenberg, Halle, Germany ----------------------------------------------------------------------------- Strain: EV484 Species: C. elegans Genotype: efIs155 II. Description: efIs155 [mex-5p::rpl-4::FLAG::tbb-2 3′UTR + Cbr-unc-119(+)] II. Tagged RPL-4 can be used for ribosome purifications from germ cells. Reference: Nousch M, et al. G3 (Bethesda). 2020 Sep 3:g3.401644.2020. doi: 10.1534/g3.120.401644 Mutagen: Outcrossed: x0 Made by: Marco Nousch Received: 12/09/20 Martin Luther University, Halle-Wittenberg, Halle, Germany ----------------------------------------------------------------------------- Strain: EV57 Species: Caenorhabditis elegans Genotype: gls-1(ef8) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Homozygous nearly sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ef8 homozygotes (nearly sterile, lays few eggs). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Rybarska A, et al. PLoS Genet. 2009 May;5(5):e1000494. Mutagen: EMS Outcrossed: x10 Made by: Agata Rybarska Received: 06/01/11 Martin Luther University, Halle-Wittenberg, Halle, Germany ----------------------------------------------------------------------------- Strain: EW10 Species: Caenorhabditis elegans Genotype: pvl-5(ga87) II. Description: Egl. Low penetrance embryonic lethal. Mutagen: EMS Outcrossed: x3 Made by: David Eisenmann Received: 02/06/01 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW12 Species: Caenorhabditis elegans Genotype: mig-14(ga62) II. Description: Egl. Low penetrance embryonic lethal. mig-14, mom-3, pvl-2, and let-553 are the same gene. Mutagen: EMS Outcrossed: x3 Made by: David Eisenmann Received: 02/06/01 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW15 Species: Caenorhabditis elegans Genotype: bar-1(ga80) X. Description: [NOTE: (10/22/2020) This strain also carries a (T to A) missense mutation in pry-1 which results in a PRY-1 N354K amino acid substitution.] bar-1(ga80) hermaphrodites are usually Egl and often have a protruding vulva (Pvl), although approx. 40% of animals appear WT on plates. Also slightly Unc. In bar-1(ga80) hermaphrodites any of the six vulval precursor cells (P3.p - P8.p) can sometimes fuse with hyp7 without dividing, and P5.p - P7.p can adopt the tertiary cell fate instead of the primary or secondary fates. In addition, the neuroblast QL and its progeny migrate towards the anterior instead of the posterior, and the cell P12 usually adopts the fate of P11. bar-1(ga80) do mate, but poorly. bar-1 encodes a beta-catenin molecule and the ga80 mutation is predicted to cause an early truncation of the protein. Mutagen: EMS Outcrossed: x>4 Made by: Dave Eisenmann Received: 12/11/98 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW23 Species: Caenorhabditis elegans Genotype: unc-104(e1265) rol-6(e187) II. Description: Dpy. Roller. Mutagen: Outcrossed: x Made by: Dave Eisenmann Received: 06/05/00 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW32 Species: Caenorhabditis elegans Genotype: lin-26(ga91) II. Description: Pvl. Egl. Mutagen: EMS Outcrossed: x3 Made by: David Eisenmann Received: 02/06/01 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW33 Species: Caenorhabditis elegans Genotype: unc-4(e120) pvl-4(ga96) II. Description: Egl. Pvl. Misshapen larvae (some lethal). Unc. Mutagen: EMS Outcrossed: x3 Made by: David Eisenmann Received: 02/06/01 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW34 Species: Caenorhabditis elegans Genotype: egl-18(ga97) IV. Description: Egl. Rol. Mutagen: EMS Outcrossed: x3 Made by: Received: 03/19/01 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW35 Species: Caenorhabditis elegans Genotype: sem-4(ga82) I. Description: Egl. Mutagen: EMS Outcrossed: x3 Made by: Received: 03/19/01 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW45 Species: Caenorhabditis elegans Genotype: smg-1(e1228) I; unc-30(e191) IV; deIs1. Description: deIs1[unc-30(+) + lin-39TL::GFP (yeast DNA)]. A translational fusion of GFP to the C terminus of LIN-39. Present on a YAC. Yeast chromosomal DNA was injected and integrated. Expression is very faint. Mutagen: Gamma Rays Outcrossed: x5 Made by: Javier Wagmaister Received: 04/12/06 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW49 Species: Caenorhabditis elegans Genotype: unc-30(e191) IV; deIs12. Description: deIs12[bar1p::GFP + pSC11(unc-30(+))]. Green worms that are non-Unc. Mutagen: Gamma Rays Outcrossed: x5 Made by: Lakshmi Natarajan Received: 04/17/06 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW5 Species: Caenorhabditis elegans Genotype: pvl-6(ga81) V. Description: Egl. Mutagen: EMS Outcrossed: x3 Made by: David Eisenmann Received: 02/06/01 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW51 Species: Caenorhabditis elegans Genotype: pvl-5(de4) II. Description: de4 is a weaker allele of pvl-5. Weakly penetrant Egl and Pvl phenotype. Low penetrance embryonic lethal and gonad migration defects. pvl-5(de4) animals have fewer Pn.p cells in the ventral midline at mid-L2 larval stage. Mutagen: EMS Outcrossed: x3 Made by: Pradeep Joshi Received: 08/26/04 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW52 Species: Caenorhabditis elegans Genotype: lin-44(n1792) I; cwn-2(ok895) IV. Description: Mutagen: Outcrossed: x Made by: Julie Gleason Received: 09/18/06 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW53 Species: Caenorhabditis elegans Genotype: lin-44(n1792) I; cwn-1(ok546) II; cwn-2(ok895) IV. Description: Mutagen: Outcrossed: x Made by: Julie Gleason Received: 09/18/06 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW55 Species: Caenorhabditis elegans Genotype: dpy-20(e1362) IV; deEx100. Description: deEx100 [ajm-1::GFP + HinfI digested complex DNA + lin-44p::4xNLS::GFP + dpy20(+)]. Pick non-Dpy to maintain. Mutagen: Outcrossed: x Made by: Julie Gleason Received: 11/15/06 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW56 Species: Caenorhabditis elegans Genotype: dpy-20(e1362) IV; deEx101. Description: deEx101[dpy20(+); ajm-1::GFP; HinfI digested complex DNA; cwn-1p::4xNLSGFP]. Pick non-Dpy to maintain. Mutagen: Outcrossed: x Made by: Julie Gleason Received: 11/15/06 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW57 Species: Caenorhabditis elegans Genotype: dpy-20(e1362) IV; deEx102. Description: deEx102[dpy20(+); ajm-1::GFP; HinfI digested complex DNA; egl-20p::4xNLSGFP]. Pick non-Dpy to maintain. Mutagen: Outcrossed: x Made by: Julie Gleason Received: 11/22/06 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW58 Species: Caenorhabditis elegans Genotype: dpy-20(e1362) IV; deEx103. Description: deEx103[dpy20(+); ajm-1::GFP; HinfI digested complex DNA; cwn-2p::4xNLSGFP]. Pick non-Dpy to maintain. Mutagen: Outcrossed: x Made by: Julie Gleason Received: 11/15/06 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW59 Species: Caenorhabditis elegans Genotype: dpy-20(e1362) IV; deEx104. Description: deEx104 [ajm-1::GFP + mom-2p::4xNLS::GFP + dpy20(+) + HinfI digested complex DNA]. Pick non-Dpy to maintain. Mutagen: Outcrossed: x Made by: Julie Gleason Received: 11/15/06 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW61 Species: Caenorhabditis elegans Genotype: dpy-20(e1282) IV; him-5(e1490) V; deIs4. Description: deIs4 [ajm-1::GFP + GFP::lin-39(YAC) + dpy-20(+)]. A transcriptional fusion of GFP to the ATG of lin-39, with a stop codon between GFP and LIN-39 sequences. Present on a YAC. Yeast chromosomal DNA was injected and integrated. Expression is very faint and may get fainter over time. Freeze upon receipt. Mutagen: Gamma Rays Outcrossed: x5 Made by: Julie Gleason Received: 04/12/06 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW62 Species: Caenorhabditis elegans Genotype: smg-1(e1228) I; dpy-20(e1282) IV; him-5(e1490) V; deIs4. Description: deIs4 [ajm-1::GFP + GFP::lin-39(YAC) + dpy-20(+)]. A transcriptional fusion of GFP to the ATG of lin-39, with a stop codon between GFP and LIN-39 sequences. Present on a YAC. Yeast chromosomal DNA was injected and integrated. Expression is very faint and may get fainter over time. Therefore, freeze upon receipt. Mutagen: Gamma Rays Outcrossed: x5 Made by: Julie Gleason Received: 04/12/06 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW68 Species: Caenorhabditis elegans Genotype: lin-44(n1792) I; cwn-1(ok546) II. Description: Mutagen: Outcrossed: x Made by: Julie Gleason Received: 09/18/06 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW72 Species: Caenorhabditis elegans Genotype: cwn-1(ok546) II; cwn-2(ok895) IV. Description: Mutagen: Outcrossed: x Made by: Julie Gleason Received: 09/18/06 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW75 Species: Caenorhabditis elegans Genotype: cwn-2(ok895) IV/nT1 [qIs51] (IV;V); dpy-11(e1180) mom-2(or42) V/nT1 [qIs51] (IV;V). Description: Heterozygotes are WT and GFP+. Segregate more WT and GFP+, Dpys, and dead eggs. Dpys give only embryonic lethals. Mutagen: Outcrossed: x Made by: Julie Gleason Received: 09/18/06 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW77 Species: Caenorhabditis elegans Genotype: lin-44(n1792) I; egl-20(n585) IV/nT1 [qIs51] (IV;V); dpy-11(e1180) mom-2(or42) V/nT1 [qIs51] (IV;V). Description: Mutagen: Outcrossed: x Made by: Julie Gleason Received: 09/18/06 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: EW79 Species: Caenorhabditis elegans Genotype: cwn-1(ok546) II; egl-20(n585) IV/nT1 [qIs51] (IV;V); dpy-11(e1180) mom-2(or42) V/nT1 [qIs51] (IV;V). Description: Heterozygotes are WT and GFP+. Segregate more WT and GFP+, Dpys, and dead eggs. Dpys give only embryonic lethals. Mutagen: Outcrossed: x Made by: Julie Gleason Received: 09/18/06 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: FA85 Species: Caenorhabditis elegans Genotype: sand-1(or552) IV. Description: Temperature-sensitve sterile. Maintain at 15-20C. Superficially wild-type at permissive temperatures; contains large bubble-like structures on the inside in all stages (described as large early endosomes), reduced brood size. Sterile and very sick at 25°C with some single escapers that are sterile and sick themselves. Suppressors have been observed after long culturing over several months, periodically check for sterility at 25C. Mutagen: EMS Outcrossed: x5 Made by: Bruce Bowerman Received: 09/28/10 Biozentrum, Basel, Switzerland ----------------------------------------------------------------------------- Strain: FAS32 Species: Caenorhabditis elegans Genotype: his-74(uge16[gfp::his-74]) V. Description: Superficially wild-type. Reference: Delaney K, et al. Genetics. 2018 Apr 10. pii: genetics.300909.2018. doi: 10.1534/genetics.118.300909. [Epub ahead of print]. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Kamila Delaney Received: 06/29/18 University of Geneva, Geneva, Switzerland ----------------------------------------------------------------------------- Strain: FAS34 Species: Caenorhabditis elegans Genotype: his-74(uge18) V. Description: Superficially wild-type. Null mutation: premature STOP codon and frame shift. Reference: Delaney K, et al. Genetics. 2018 Apr 10. pii: genetics.300909.2018. doi: 10.1534/genetics.118.300909. [Epub ahead of print]. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Kamila Delaney Received: 06/29/18 University of Geneva, Geneva, Switzerland ----------------------------------------------------------------------------- Strain: FAS43 Species: Caenorhabditis elegans Genotype: his-69&his-70(uge44) his-72(tm2066) III; his-74(uge18) V; his-71(ok2289) X. Description: Deletion of all genes coding H3.3 histone variant. Superficially wild-type with slight reduction of brood size at 25C. Reference: Delaney K, et al. Genetics. 2018 Apr 10. pii: genetics.300909.2018. doi: 10.1534/genetics.118.300909. [Epub ahead of print]. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Kamila Delaney Received: 06/29/18 University of Geneva, Geneva, Switzerland ----------------------------------------------------------------------------- Strain: FAS46 Species: Caenorhabditis elegans Genotype: his-72 (uge30[gfp::his-72]) III. Description: Superficially wild-type. Reference: Delaney K, et al. Genetics. 2018 Apr 10. pii: genetics.300909.2018. doi: 10.1534/genetics.118.300909. [Epub ahead of print]. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Kamila Delaney Received: 06/29/18 University of Geneva, Geneva, Switzerland ----------------------------------------------------------------------------- Strain: FAS47 Species: Caenorhabditis elegans Genotype: his-70(uge31[gfp::his-70]) III. Description: Superficially wild-type. Reference: Delaney K, et al. Genetics. 2018 Apr 10. pii: genetics.300909.2018. doi: 10.1534/genetics.118.300909. [Epub ahead of print]. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Kamila Delaney Received: 06/29/18 University of Geneva, Geneva, Switzerland ----------------------------------------------------------------------------- Strain: FAS65 Species: Caenorhabditis elegans Genotype: his-69&his-70(uge44) III. Description: Superficially wild-type. Deletion of his-69 and his-70; complex substitution with an insertion at break site: aacaaatcagttctcacttttagcc-TCTTGGATTTAATAAATAAATTA-agtttaagtttccgccaatgaaaaa. Reference: Delaney K, et al. Genetics. 2018 Apr 10. pii: genetics.300909.2018. doi: 10.1534/genetics.118.300909. [Epub ahead of print]. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Kamila Delaney Received: 06/29/18 University of Geneva, Geneva, Switzerland ----------------------------------------------------------------------------- Strain: FAS84 Species: Caenorhabditis elegans Genotype: his-71(uge45[gfp::his-71]) X. Description: Superficially wild-type. Reference: Delaney K, et al. Genetics. 2018 Apr 10. pii: genetics.300909.2018. doi: 10.1534/genetics.118.300909. [Epub ahead of print]. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Kamila Delaney Received: 06/29/18 University of Geneva, Geneva, Switzerland ----------------------------------------------------------------------------- Strain: FC121 Species: Caenorhabditis elegans Genotype: zzIs16. Description: zzIs16 [(pJE3) eff-1p::GFP + rol-6(su1006)]. GFP+ Rollers. Chromosomal insertion of zzEx10. Integration site of zzIs16 not yet mapped, but it is not tightly linked to eff-1 II, unc-119 III, or jcIs1 IV. pJE3 has 7.5 kb of eff-1 upstream sequence inserted into pPD95.75, driving cytoplasmic GFP expression. Mutagen: Outcrossed: x6 Made by: E. Opoku-Serebuoh Received: 10/17/03 University of Connecticut, Farmington, CT ----------------------------------------------------------------------------- Strain: FC183 Species: Caenorhabditis elegans Genotype: zzIs22. Description: zzIs22 [(pJdC41) (eff-1::GFP) + rol-6(su1006)]. Rollers. All worms have tail whips. Mutagen: Outcrossed: x2 Made by: Jake del Campo Received: 04/01/05 University of Connecticut, Farmington, CT ----------------------------------------------------------------------------- Strain: FC50 Species: Caenorhabditis elegans Genotype: zzEx10. Description: zzEx10 [(pJE3) eff-1p::GFP + rol-6(su1006)]. Pick Rollers to maintain. eff-1p expression is observed in epithelia committed to fusion and in fused syncytia. Mutagen: Outcrossed: x Made by: WA Mohler Received: 08/25/03 Technion Research and Development Foundation, Haifa, Israel ----------------------------------------------------------------------------- Strain: FDU1056 Species: C. elegans Genotype: mig-17(shc19[mig-17::mNG +LoxP]) V. Description: C-terminus of endogenous mig-17 locus tagged with mNeonGreen using CRISPR/Cas9. Reference: Fan J, et al. Elife. 2020 Apr 7;9:e55890. PMID: 32255430 Mutagen: Outcrossed: x0 Made by: Zhiyong Shao Received: 03/18/21 Duke University ----------------------------------------------------------------------------- Strain: FF1 Species: Caenorhabditis elegans Genotype: dpy-5(f1) I. Description: Dpy. Semi-sterile. Bergerac background. [NOTE: Presumably sterile at 25°C because of temperature-sensitive zyg-12(ct350) in the background (Malone et al., Cell 2003).] Mutagen: Spontaneous Outcrossed: x Made by: Michel Dion Received: 10/20/80 Universite Claude Bernard (Lyon-I), Lyon, France ----------------------------------------------------------------------------- Strain: FF10 Species: Caenorhabditis elegans Genotype: dpy-6(f10) X. Description: Dpy. Semi-sterile. Bergerac background. [NOTE: Presumably sterile at 25°C because of temperature-sensitive zyg-12(ct350) in the background (Malone et al., Cell 2003).] Mutagen: X-ray Outcrossed: x Made by: Roland Ouazana Received: 09/01/80 Universite Claude Bernard (Lyon-I), Lyon, France ----------------------------------------------------------------------------- Strain: FF11 Species: Caenorhabditis elegans Genotype: dpy-6(f11) X. Description: Dpy. Bergerac background. [NOTE: Presumably sterile at 25°C because of temperature-sensitive zyg-12(ct350) in the background (Malone et al., Cell 2003).] Mutagen: X-ray Outcrossed: x Made by: Ouazana R Received: 09/01/80 Universite Claude Bernard (Lyon-I), Lyon, France ----------------------------------------------------------------------------- Strain: FF14 Species: Caenorhabditis elegans Genotype: dpy-1(f14) III. Description: Dpy. Bergerac background. [NOTE: Presumably sterile at 25°C because of temperature-sensitive zyg-12(ct350) in the background (Malone et al., Cell 2003).] Mutagen: EMS Outcrossed: x Made by: Ouazana R Received: 10/01/80 Universite Claude Bernard (Lyon-I), Lyon, France ----------------------------------------------------------------------------- Strain: FF17 Species: Caenorhabditis elegans Genotype: dpy-6(f17) X. Description: Dpy. Bergerac background. [NOTE: Presumably sterile at 25°C because of temperature-sensitive zyg-12(ct350) in the background (Malone et al., Cell 2003).] Mutagen: X-ray Outcrossed: x Made by: Ouazana R Received: 10/01/80 Universite Claude Bernard (Lyon-I), Lyon, France ----------------------------------------------------------------------------- Strain: FF18 Species: Caenorhabditis elegans Genotype: dpy-6(f18) X. Description: Dpy. Bergerac background. [NOTE: Presumably sterile at 25°C because of temperature-sensitive zyg-12(ct350) in the background (Malone et al., Cell 2003).] Mutagen: X-ray Outcrossed: x Made by: Ouazana R Received: 10/01/80 Universite Claude Bernard (Lyon-I), Lyon, France ----------------------------------------------------------------------------- Strain: FF275 Species: Caenorhabditis elegans Genotype: dpy-17(e164) unc-32(f123) ncl-1(e1865)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, Dpy Steriles (qC1 homozygotes), and embryonic lethals (f123 homozygotes) Mutagen: EMS Outcrossed: x Made by: Pujol/Thierry-Mieg Received: 05/24/04 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: FF276 Species: Caenorhabditis elegans Genotype: dpy-17(e164) unc-32(f121) ncl-1(e1865)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, Dpy Steriles (qC1 homozygotes), and larval lethals (f121 homozygotes). g to a transition in exon 6 (2874), changing a Gly in Glu in ZK637.8 gene encoding a V-ATPase alpha subunit Mutagen: EMS Outcrossed: x Made by: Pujol/Thierry-Mieg Received: 05/24/04 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: FF41 Species: Caenorhabditis elegans Genotype: unc-116(e2310) III. Description: Hypomorphic allele. Fully viable and fertile. Strongly uncoordinated larvae, tend to coil backward. Dpyish, mildly Unc adults. Isolated out of a mutator strain also containing uncharacterized e2281 and e2282. Mutagen: Spontaneous in TR679 Outcrossed: x15 Made by: Thierry-Mieg Received: 01/14/94 NCBI, Bethesda, MD ----------------------------------------------------------------------------- Strain: FF451 Species: Caenorhabditis elegans Genotype: unc-32(f131) III. Description: Extreme coiler from L1 to adult. Hypomorph. f131/nDf17 is L1 lethal. Molecular lesion: g to a transition in splice donor site of exon 6 (g3485a) in ZK637.8 gene encoding a V-ATPase alpha subunit. Mutagen: EMS Outcrossed: x3 Made by: Nathalie Pujol Received: 01/17/01 NCBI, Bethesda, MD ----------------------------------------------------------------------------- Strain: FF628 Species: Caenorhabditis elegans Genotype: let-756(s2613) unc-32(e189) III. Description: Homozygous viable and fertile, but slow growing, transparent and small. Originally from BC4253 but outcrossed an additional 7 times. Mutagen: EMS Outcrossed: x7 Made by: D. Thierry-Mieg Received: 05/17/99 NCBI, Bethesda, MD ----------------------------------------------------------------------------- Strain: FG125 Species: Caenorhabditis elegans Genotype: ocr-2(ak47) osm-9(ky10) IV; ocr-1(ak46) V. Description: Chemosensory, mechanosensory, and osmosensory defects. Mutagen: Outcrossed: x Made by: Meredith Scheider Received: 04/13/09 State University of New York, Buffalo, NY ----------------------------------------------------------------------------- Strain: FG58 Species: Caenorhabditis elegans Genotype: dop-4(tm1392) X. Description: Mutagen: Outcrossed: x5 Made by: Denise Ferkey Received: 07/24/08 State University of New York, Buffalo, NY ----------------------------------------------------------------------------- Strain: FG7 Species: Caenorhabditis elegans Genotype: grk-2(gk268) III. Description: Mutagen: Outcrossed: x8 Made by: Meredith Scheider Received: 07/24/08 State University of New York, Buffalo, NY ----------------------------------------------------------------------------- Strain: FG927 Species: C. elegans Genotype: inx-1(tm6662) X. Description: Derived by out-crossing parental strain FX18537 five times. Mutagen: Outcrossed: x5 Made by: Savannah Sojka Received: 11/21/22 State University of New York, Buffalo, NY ----------------------------------------------------------------------------- Strain: FG928 Species: C.elegans Genotype: inx-1(tm3524) X. Description: Derived by out-crossing parental strain FX18538 five times. Mutagen: Outcrossed: x5 Made by: Savannah Sojka Received: 11/21/22 State University of New York, Buffalo, NY ----------------------------------------------------------------------------- Strain: FG929 Species: C.elegans Genotype: inx-17(tm3292) I. Description: Derived by out-crossing parental strain FX3292 five times. Mutagen: Outcrossed: x5 Made by: Savannah Sojka Received: 11/21/22 State University of New York, Buffalo, NY ----------------------------------------------------------------------------- Strain: FG981 Species: C. elegans Genotype: unc-7(e5) X. Description: Derived by out-crossing parental strain CB5. Mutagen: Outcrossed: x5 Made by: Savannah Sojka Received: 11/21/22 State University of New York, Buffalo, NY ----------------------------------------------------------------------------- Strain: FG982 Species: C. elegans Genotype: inx-17(tm3839) I. Description: Derived by out-crossing parental strain FX3839 five times. Mutagen: Outcrossed: x5 Made by: Savannah Sojka Received: 11/21/22 State University of New York, Buffalo, NY ----------------------------------------------------------------------------- Strain: FGP1 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; fgpIs20. Description: fgpIs20 [(pFGP79) pie-1p::mCherry::smo-1(GG) + unc-119(+)]. Propagate at 20-25°C. Switching to 25C prior to imaging may enhance the signal from fgpIs20. Allows SUMO localization/conjugation to be observed within the germline and embryos. Reference: Pelisch et al. Nat Commun. 2014 Dec 5;5:5485. Mutagen: Outcrossed: x0 Made by: Federico Pelisch Received: 10/21/14 University of Dundee, Dundee, UK ----------------------------------------------------------------------------- Strain: FGP2 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; fgpIs21. Description: fgpIs21 [(pFGP80) pie-1p::mCherry::smo-1(GA) + unc-119(+)]. Propagate at 20-25°C. Switching to 25C prior to imaging may enhance the signal. May be used to follow unconjugated SUMO localization within the germline and embryo. When compared with strain FGP1, the difference in fluorescence signal corresponds to SUMO conjugation in FGP1. Reference: Pelisch et al. Nat Commun. 2014 Dec 5;5:5485. Mutagen: Outcrossed: x0 Made by: Federico Pelisch Received: 10/21/14 University of Dundee, Dundee, UK ----------------------------------------------------------------------------- Strain: FGP29 Species: Caenorhabditis elegans Genotype: gei-17(fgp1[GFP::FLAG::degron::loxP::gei-17]) I; ieSi38 IV. Description: gei-17(fgp1[GFP::FLAG::degron::loxP::gei-17]) I. ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. Single copy transgene inserted into chromosome IV (cxTi10882) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in germ line and early embryos. Reference: Pelisch et al. Mol Cell. 2017 Jan 5;65(1):66-77. Mutagen: Outcrossed: x0 Made by: Federico Pelisch Received: 01/30/17 University of Dundee, Dundee, UK ----------------------------------------------------------------------------- Strain: FGP3 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; fgpIs23. Description: fgpIs23 [(pFGP78) pie-1p::GFP::TEV-S-Tag::smo-1(GG) + unc-119(+)]. Propagate at 20-25°C. Switching to 25C prior to imaging may enhance the signal. Allows SUMO localization/conjugation to be observed within the germline and embryos. Reference: Pelisch et al. Nat Commun. 2014 Dec 5;5:5485. Mutagen: Outcrossed: x0 Made by: Federico Pelisch Received: 10/21/14 University of Dundee, Dundee, UK ----------------------------------------------------------------------------- Strain: FGP30 Species: Caenorhabditis elegans Genotype: gei-17(fgp1[GFP::FLAG::degron::loxP::gei-17]) I; ltIs37 IV. Description: gei-17(fgp1[GFP::FLAG::degron::loxP::gei-17]) I. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Pelisch et al. Mol Cell. 2017 Jan 5;65(1):66-77. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Federico Pelisch Received: 01/30/17 University of Dundee, Dundee, UK ----------------------------------------------------------------------------- Strain: FGP4 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; fgpIs24. Description: fgpIs24 [(pFGP77) pie-1p::GFP::TEV-S-Tag::smo-1(GA) + unc-119(+)]. Propagate at 20-25°C. Switching to 25C prior to imaging may enhance the signal. When compared with strain FGP3, the difference in fluorescence signal corresponds to SUMO conjugation in FGP3. Reference: Pelisch et al. Nat Commun. 2014 Dec 5;5:5485. Mutagen: Outcrossed: x0 Made by: Federico Pelisch Received: 10/21/14 University of Dundee, Dundee, UK ----------------------------------------------------------------------------- Strain: FGP7 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; ruIs57; fgpIs20. Description: ruIs57 [pie-1p::GFP::tubulin + unc-119(+)]. Expression of GFP::tubulin fusion in germline and early embryos. fgpIs20 [(pFGP79) pie-1p::mCherry::smo-1(GG) + unc-119(+)]. Propagate at 20-25°C. Switching to 25C prior to imaging may enhance the signal from fgpIs20. Allows SUMO localization/conjugation to be observed within the germline and embryos. Reference: Pelisch et al. Nat Commun. 2014 Dec 5;5:5485. Mutagen: Outcrossed: x0 Made by: Federico Pelisch Received: 10/21/14 University of Dundee, Dundee, UK ----------------------------------------------------------------------------- Strain: FGP8 Species: Caenorhabditis elegans Genotype: unc-119(ed3) ruIs32 III; fgpIs20. Description: ruIs32 [pie-1p::GFP::H2B + unc-119(+)] III. Expression of GFP::H2B histone fusion in germline. fgpIs20 [(pFGP79) pie-1p::mCherry::smo-1(GG) + unc-119(+)]. Propagate at 20-25°C. Switching to 25C prior to imaging may enhance the signal from fgpIs20. Allows SUMO localization/conjugation to be observed within the germline and embryos. Reference: Pelisch et al. Nat Commun. 2014 Dec 5;5:5485. Mutagen: Outcrossed: x0 Made by: Federico Pelisch Received: 10/21/14 University of Dundee, Dundee, UK ----------------------------------------------------------------------------- Strain: FH85 Species: Caenorhabditis elegans Genotype: unc-9(ec27) X. Description: Unc. Males are fertile. Mutagen: EMS Outcrossed: x2 Made by: M. Sedensky Received: 01/01/87 Haverford College, Haverford PA, USA ----------------------------------------------------------------------------- Strain: FJ1519 Species: C. elegans Genotype: cdk-5(gm336) III. Description: cdk-5(gm336) mutants exhibit defects in polarized trafficking of dense-core vesicles in motor neurons, and decreased localization of GLR-1::GFP to puncta in ventral nerve cord interneurons. Note that this strain does not contain GLR-1::GFP. References: Juo P, et al. Mol Biol Cell. 2007 Oct;18(10):3883-93. Goodwin PR, et al. J Neurosci. 2012 Jun 13;32(24):8158-72. Mutagen: Outcrossed: x6 Made by: Gian Garriga Received: 03/01/18 Tufts University, Boston, MA ----------------------------------------------------------------------------- Strain: FJ224 Species: Caenorhabditis elegans Genotype: unc-31(e298) IV; dpy-11(e224) V. Description: Mutagen: Outcrossed: x3 Made by: J. Kowalski Received: 05/21/07 Tufts University, Boston, MA ----------------------------------------------------------------------------- Strain: FK100 Species: Caenorhabditis elegans Genotype: tax-2(ks10) I. Description: Mutagen: EMS Outcrossed: x3 Made by: Ikue Mori Received: 09/15/05 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: FK101 Species: Caenorhabditis elegans Genotype: tax-2(ks15) I. Description: Mutagen: EMS Outcrossed: x3 Made by: Ikue Mori Received: 09/15/05 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: FK103 Species: Caenorhabditis elegans Genotype: tax-4(ks28) III. Description: Mutagen: EMS Outcrossed: x3 Made by: Ikue Mori Received: 09/15/05 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: FK104 Species: Caenorhabditis elegans Genotype: tax-2(ks31) I. Description: Mutagen: EMS Outcrossed: x3 Made by: Ikue Mori Received: 09/15/05 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: FK129 Species: Caenorhabditis elegans Genotype: tax-4(ks11) III. Description: Mutagen: EMS Outcrossed: x3 Made by: Ikue Mori Received: 09/15/05 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: FK134 Species: Caenorhabditis elegans Genotype: ttx-3(ks5) X. Description: Abnormal in thermotaxis. Cryophilic (cold-seeking) and abnormal in isothermal tracking. Mutagen: EMS Outcrossed: x4 Made by: Yamashita/Mori Received: 04/04/96 Sojo University, Ikeda, Kumamoto, Japan ----------------------------------------------------------------------------- Strain: FK163 Species: Caenorhabditis elegans Genotype: cam-1(ks52) II. Description: Deletion of the tyrosine kinase domain of kin-8. Partial Daf-c especially on an old lawn of E. coli. Reduced daf-7 expression in ASI. Dye-filling defective in ASI. Abnormal ASI cell position. 10-20% of animals show withered (Wit) tail phenotype or defects in elongation or migration of posterior gonad. Previously called kin-8. Mutagen: Tc1 Outcrossed: x3 Made by: Makoto Koga Received: 03/13/00 Sojo University, Ikeda, Kumamoto, Japan ----------------------------------------------------------------------------- Strain: FK171 Species: Caenorhabditis elegans Genotype: mek-1(ks54) sek-1(qd127) X. Description: Hypersensitive to copper and cadmium ions, and to starvation. [NOTE: Prior studies suggested possible cross-regulation of PMK-1 by MEK-1, but the identification of a tightly linked partial loss-of-function mutation in sek-1(qd127) in the strain carrying the mek-1(ks54) mutant allele (K. Reddy and D. Kim, unpublished data) suggests that the diminished PMK-1 activation observed in this mutant strain may actually be due to diminished SEK-1 activity.] Mutagen: Tc1 Outcrossed: x2 Made by: Makoto Koga Received: 01/22/01 Sojo University, Ikeda, Kumamoto, Japan ----------------------------------------------------------------------------- Strain: FK181 Species: Caenorhabditis elegans Genotype: ksIs2. Description: ksIs2 [daf-7p::GFP + rol-6(su1006)]. Rollers. Mutagen: Outcrossed: x Made by: Received: 02/14/05 Sojo University, Ikeda, Kumamoto, Japan ----------------------------------------------------------------------------- Strain: FK183 Species: Caenorhabditis elegans Genotype: daf-11(ks67) V; ksEx29. Description: ksEx29 [daf-7::GFP + lin-44::GFP]. Maintain by picking GFP. daf-7::GFP is dark or invisible. lin-44::GFP is bright in the tail. Grows better at 15C. Mutagen: Outcrossed: x Made by: Makoto Koga Received: 01/23/07 Sojo University, Ikeda, Kumamoto, Japan ----------------------------------------------------------------------------- Strain: FK223 Species: Caenorhabditis elegans Genotype: egl-4(ks60) IV. Description: Larger body size, longer lifespan, egg-laying defect, male tail abnormal. Mutagen: EMS Outcrossed: x3 Made by: Y Nakano & Y Ohshima Received: 09/16/03 Sojo University, Ikeda, Kumamoto, Japan ----------------------------------------------------------------------------- Strain: FK229 Species: Caenorhabditis elegans Genotype: egl-4(ks61) IV. Description: Larger body size, longer lifespan, egg-laying defect, male tail abnormal. Mutagen: EMS Outcrossed: x3 Made by: Y Nakano & Y Ohshima Received: 09/16/03 Sojo University, Ikeda, Kumamoto, Japan ----------------------------------------------------------------------------- Strain: FK234 Species: Caenorhabditis elegans Genotype: egl-4(ks62) IV. Description: Larger body size, longer lifespan, egg-laying defect, male tail abnormal. Mutagen: EMS Outcrossed: x2 Made by: Y Nakano & Y Ohshima Received: 09/16/03 Sojo University, Ikeda, Kumamoto, Japan ----------------------------------------------------------------------------- Strain: FK311 Species: Caenorhabditis elegans Genotype: ceh-36(ks86) X. Description: Defective chemotaxis to Na+ and isoamyl alcohol. Mutagen: EMS Outcrossed: x1 Made by: Makoto Koga Received: 04/19/04 Sojo University, Ikeda, Kumamoto, Japan ----------------------------------------------------------------------------- Strain: FK312 Species: Caenorhabditis elegans Genotype: sma-5(n678) X. Description: Made by crossing MT3353 egl-15(n484) sma-5(n678) with N2 males and selecting animals that grew much better. FK312 probably only carries the sma-5(n678) mutation but that has not been confirmed by sequencing. Small body size, slow growth, abnormal intestinal granules, shorter lifespan than WT. Mutagen: Outcrossed: x Made by: Yasumi Ohshima Received: 03/01/07 Sojo University, Ikeda, Kumamoto, Japan ----------------------------------------------------------------------------- Strain: FK410 Species: Caenorhabditis elegans Genotype: sma-2(e502) III; sma-5(n678) X. Description: Very small. Small brood size (about 1/10 that of WT). Grows very slowly. Reference: Watanabe, N et al. Genes to Cells 2007. 12(5):603-609. Mutagen: Outcrossed: x Made by: Y Ohshima Received: 10/30/08 Sojo University, Ikeda, Kumamoto, Japan ----------------------------------------------------------------------------- Strain: FK412 Species: Caenorhabditis elegans Genotype: sma-4(e729) III; sma-5(n678) X. Description: Very small. Small brood size (about 1/10 that of WT). Grows very slowly. Line A. Reference: Watanabe, N et al. Genes to Cells 2007. 12(5):603-609. Mutagen: Outcrossed: x Made by: Y Ohshima Received: 10/30/08 Sojo University, Ikeda, Kumamoto, Japan ----------------------------------------------------------------------------- Strain: FK413 Species: Caenorhabditis elegans Genotype: sma-4(e729) III; sma-5(n678) X. Description: Very small. Small brood size (about 1/10 that of WT). Grows very slowly. Line C. Reference: Watanabe, N et al. Genes to Cells 2007. 12(5):603-609. Mutagen: Outcrossed: x Made by: Y Ohshima Received: 10/30/08 Sojo University, Ikeda, Kumamoto, Japan ----------------------------------------------------------------------------- Strain: FK430 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n309) X; ksEx30. Description: ksEx30 [dyf-3::GFP + lin-15(+)]. Mutagen: Outcrossed: x Made by: T. Murayama Received: 03/12/05 Sojo University, Ikeda, Kumamoto, Japan ----------------------------------------------------------------------------- Strain: FN64 Species: Caenorhabditis elegans Genotype: fnEx4. Description: fnEx4 [unc-85::GFP + rol-6(su1006)]. Rollers. GFP highly expressed in embryos, VPNs in L1, Intestine in L2, VPNs in L3-L4. Maintain by picking GFP+ rollers. Reference: Grigsby IF & Finger FP. (2008) Dev Biol 319(1):100-9. Mutagen: Outcrossed: x Made by: Iwen Fu Received: 05/20/10 Rensselaer Polytechnic Institute, Troy, NY ----------------------------------------------------------------------------- Strain: FQ63 Species: C. elegans Genotype: lin-15AB(n765) X; wzIs20. Description: wzIs20 [lgc-55p::GFP + lin-15(+)]. lgc-55 regulatory sequences drive expression of GFP in neurons, GLR cells and some muscles. Reference: Ringstad N, et al. Science. 2009;325(5936):96-100. doi:10.1126/science.1169243 Mutagen: gamma irradiation Outcrossed: x2 Made by: Niels Ringstad Received: 06/03/24 NYU Medical Center, New York, NY ----------------------------------------------------------------------------- Strain: FR431 Species: Caenorhabditis elegans Genotype: ceh-13(sw1)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, Dpy Steriles and ceh-13 homozygotes which are dead eggs and deformed embryos. Mutagen: Outcrossed: x10 Made by: Karen Brunschwig Received: 10/13/99 University of Fribourg, Switzerland ----------------------------------------------------------------------------- Strain: FT1197 Species: C. elegans Genotype: unc-119(ed3); xnIs449. Description: xnIs449 [lin-26::lifeAct::GFP + unc-119(+)]. LifeAct-GFP expressed in epidermal cells of embryos. In adults, expression is also observed in pharynx and some cells in the tail. Reference: Zilberman, Y., J. Abrams, D.C. Anderson, and J. Nance. 2017. Cdc42 regulates junctional actin but not cell polarization in the Caenorhabditis elegans epidermis. J Cell Biol 216: 3729-3744. Mutagen: Outcrossed: x0 Made by: Yuliya Zilberman Received: 11/13/17 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT1221 Species: Caenorhabditis elegans Genotype: unc-119(ed3) xnIs459 III. Description: xnIs459 [YFP::ral-1a + unc-119(+)] III. Expresses YFP::ral-1 maternally and zygotically. Expression is present in many cells, including early embryos, epithelial cells, the excretory cell, and the germ line. Reference: Armenti ST, Chan E, Nance J. Dev Biol. 2014 Aug 4. pii: S0012-1606(14)00355-8. Mutagen: Bombardment Outcrossed: x0 Made by: Stephen Armenti Received: 08/25/14 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT1265 Species: Caenorhabditis elegans Genotype: sec-5(pk2358) II; xnIs461. Description: xnIs461 [sec-5::YFP + unc-119(+)]. Expresses sec-5::YFP maternally and zygotically. Expression is present in many cells, including early embryos, epithelial cells, the excretory cell, and the germ line. Rescues maternal-effect lethality of sec-5(pk2358). Reference: Armenti ST, Chan E, Nance J. Dev Biol. 2014 Aug 4. pii: S0012-1606(14)00355-8. Mutagen: Bombardment Outcrossed: x0 Made by: Stephen Armenti Received: 08/25/14 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT1310 Species: Caenorhabditis elegans Genotype: avr-14(ad1302) I; xnSi31 II; unc-119 (ed3) III; glc-1(pk54::Tc1) avr-15(ad1051) V. Description: xnSi31 [sec-8::mCherry + unc-119(+)] II. Expresses sec-8::mCherry maternally and zygotically. Expression is present in many cells, including early embryos, epithelial cells, the excretory cell, and the germ line. Transgene was inserted by MosSCI into ttTi5605 excision site. Derived from WM186. Reference: Armenti ST, Chan E, Nance J. Dev Biol. 2014 Aug 4. pii: S0012-1606(14)00355-8. Mutagen: MosSCI Outcrossed: x0 Made by: Stephen Armenti Received: 08/25/14 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT1341 Species: Caenorhabditis elegans Genotype: xnIs23. Description: xnIs23 [cdc-42p::zf1::GFP::cdc-42 + unc-119(+)]. Transgene insertion expressing ZF1::GFP::CDC-42 ubiquitously in somatic cells. Might still have unc-119(ed3) in the background. Reference: Armenti ST, et al. Development 2014 (in press). Mutagen: Bombardment Outcrossed: x1 Made by: Jeremy Nance Received: 10/07/14 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT1370 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; xnIs484. Description: xnIs484 [mCherry::sec-10 + unc-119(+)]. Expresses mCherry::sec-10 maternally and zygotically. Expression is present in many cells, including early embryos, epithelial cells, the excretory cell, and the germ line. Reference: Armenti ST, Chan E, Nance J. Dev Biol. 2014 Aug 4. pii: S0012-1606(14)00355-8. Mutagen: Bombardment Outcrossed: x0 Made by: Stephen Armenti Received: 08/25/14 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT1379 Species: Caenorhabditis elegans Genotype: avr-14(ad1302) I; xnSi34 II; unc-119 (ed3) III; glc-1(pk54::Tc1) avr-15(ad1051) V. Description: xnSi34 [sec-15::YFP + unc-119(+)] II. Expresses sec-15::YFP maternally and zygotically. Expression is present in many cells, including early embryos, epithelial cells, the excretory cell, and the germ line. Transgene was inserted by MosSCI into ttTi5605 excision site. Derived from WM186. Reference: Armenti ST, Chan E, Nance J. Dev Biol. 2014 Aug 4. pii: S0012-1606(14)00355-8. Mutagen: MosSCI Outcrossed: x0 Made by: Stephen Armenti Received: 08/25/14 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT1394 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; xnIs502. Description: xnIs502 [pie-1p::GFP::jac-1 + unc-119(+)]. Maintain at 25C to slow silencing of transgene. GFP::JAC-1 localized at contacts in early embryos. Reference: Klompstra D, et al. Nat Cell Biol. 2015 Jun;17(6):726-35. Mutagen: Outcrossed: x0 Made by: Diana Klompstra Received: 08/17/15 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT1450 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; xnIs23; xnEx342. Description: xnIs23 [cdc-42p::ZF1::GFP::cdc-42 + unc-119(+)]. xnEx342 [rab-3p::zif-1 + rab-3p::mCherry + rol-6(su1006)]. Pick Rollers to maintain. ZF1::GFP::cdc-42 is expressed ubiquitously and enriched at the plasma membrane. In embryos inheriting xnEx342, ZF1::GFP::cdc-42 is degraded and mCherry is expressed in neurons. Reference: Armenti ST, et al. Development. 2014 Dec;141(23):4640-7. Mutagen: Outcrossed: x0 Made by: Stephen Armenti Received: 01/06/15 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT1459 Species: C. elegans Genotype: unc-119(ed3); xnIs506. Description: xnIs506 [cdc42p::GST::GFP::wsp-1(GBD) + unc-119(+)]. Biosensor for active CDC-42. In epithelia, GFP is enriched at junctions between epithelial cells. Some signal is in the nucleus (this signal is reduced by addition of GST, which makes the transgenic protein larger). Reference: Zilberman, Y., J. Abrams, D.C. Anderson, and J. Nance. 2017. Cdc42 regulates junctional actin but not cell polarization in the Caenorhabditis elegans epidermis. J Cell Biol 216: 3729-3744. Mutagen: Outcrossed: x0 Made by: Yuliya Zilberman Received: 11/13/17 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT1481 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; xnIs23; xnEx350. Description: xnIs23 [cdc-42p::ZF1::GFP::cdc-42 + unc-119(+)]. xnEx350 [elt-2p::zif-1 + elt-2p::mCherry + rol-6(su1006)]. Pick Rollers to maintain. ZF1::GFP::cdc-42 is expressed ubiquitously and enriched at the plasma membrane. In embryos inheriting xnEx350, ZF1::GFP::cdc-42 is degraded and mCherry is expressed in the intestine. Reference: Armenti ST, et al. Development. 2014 Dec;141(23):4640-7. Mutagen: Outcrossed: x0 Made by: Stephen Armenti Received: 01/06/15 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT1523 Species: Caenorhabditis elegans Genotype: sec-5(xn51[sec-5::zf1::YFP + LoxP::unc-119::LoxP]) II; unc-119(ed3) III. Description: sec-5(xn51 [sec-5::zf1::YFP + LoxP::unc-119::LoxP]) II. Knock-in of zf1::yfp and unc-119(+) into the sec-5 locus. Expresses SEC-5::ZF1::YFP maternally and zygotically. Expression in many cells, including early embryos, epithelial cells, excretory cell, and germ line. unc-119 is present in reverse orientation within an intron of YFP. Reference: Armenti ST, et al. Development 2014 (in press). Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Stephen Armenti Received: 10/07/14 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT1547 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; xnIs23; xnEx380. Description: xnIs23 [cdc-42p::ZF1::GFP::cdc-42 + unc-119(+)]. xnEx380 [hsp-16.41p::zif-1::SL2::mCherry + rol-6(su1006)]. Pick Rollers to maintain. ZF1::GFP::cdc-42 is expressed ubiquitously and enriched at the plasma membrane. In embryos inheriting xnEx380, ZF1::GFP::cdc-42 is degraded and mCherry is expressed after heatshock. Reference: Armenti ST, et al. Development. 2014 Dec;141(23):4640-7. Mutagen: Outcrossed: x0 Made by: Stephen Armenti Received: 01/06/15 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT1568 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; xnIs371; xnEx384. Description: xnIs371 [pie-1p::GFP::pac-1(1-574) + unc-119(+)]. xnEx384 [hsp16p::HA::phplc1(delta)1::hmr-1(ICD) + rol-6(su1006)]. Rollers. Rollers express HMR-1 intracellular domain pan-cortically in cells upon heat shock. Maintain at 15C or 20C to prevent leaky expression of heat shock transgene. Reference: Klompstra D, et al. Nat Cell Biol. 2015 Jun;17(6):726-35. Mutagen: Outcrossed: x0 Made by: Diana Klompstra Received: 08/17/15 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT1569 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; xnIs371; xnEx385. Description: xnIs371 [pie-1p::GFP::pac-1(1-574) + unc-119(+)]. xnEx385 [hsp16p::HA::phplc1(delta)1::hmr-1(ICD-M) + rol-6(su1006)]. Rollers. Rollers express mutated HMR-1 intracellular domain (unable to bind catenins) pan-cortically in cells upon heat shock. Maintain at 15C or 20C to prevent leaky expression of heat shock transgene. Reference: Klompstra D, et al. Nat Cell Biol. 2015 Jun;17(6):726-35. Mutagen: Outcrossed: x0 Made by: Diana Klompstra Received: 08/17/15 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT1607 Species: Caenorhabditis elegans Genotype: xnIs520. Description: xnIs520 [cdc-42p::zif-1 + cdc-42p::mCherry]. Ubiquitous ZIF-1 and mCherry in somatic cells. Reference: Armenti ST, et al. Development 2014 (in press). Mutagen: TMP/UV Outcrossed: x2 Made by: Jeremy Nance Received: 10/07/14 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT1609 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; xnIs528. Description: xnIs528 [hmp-1p::hmp-1::GFP + unc-119(+)]. HMP-1::GFP localized to cell contacts in early embryo and junctions in later stages. Reference: Klompstra D, et al. Nat Cell Biol. 2015 Jun;17(6):726-35. Mutagen: Outcrossed: x0 Made by: Jeremy Nance Received: 08/17/15 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT17 Species: Caenorhabditis elegans Genotype: xnIs3 unc-119(ed3) III. Description: xnIs3[par-6:PAR-6::GFP + unc-119(+)]. Wild type worms. Express PAR-6::GFP in early embryos and epithelial cells. Mutagen: Outcrossed: x Made by: Jeremy Nance Received: 04/05/07 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT183 Species: Caenorhabditis elegans Genotype: cdc-42(gk388) II; unc-119(ed3) III; xnIs78. Description: xnIs78 [cdc-42p::2xHA::cdc-42 + unc-119(+)]. Superficially wild-type. Reference: Anderson DC, et al. (2008) Science 320(5884):1771-4. Mutagen: Bombardment Outcrossed: x Made by: D Anderson/J Nance Received: 04/05/10 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT204 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; xnIs87. Description: xnIs87 [syn-4p::GFP::syn-4::syn-4 3'UTR + unc-119(+)]. Mutagen: Bombardment Outcrossed: x0 Made by: Ann Wehman Received: 07/19/09 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT2064 Species: C. elegans Genotype: hmg-5(xn107[hmg-5::gfp]) IV. Description: GFP-tagged HMG-5/TFAM labels mtDNA nucleoids. GFP tag causes a reduction in number of mtDNAs. Reference: Schwartz AZA, et al. eLife. 2022 Oct 6:11:e80396. doi: 10.7554/eLife.80396. PMID: 36200990. Mutagen: Outcrossed: x0 Made by: Aaron Schwartz Received: 04/10/24 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT207 Species: Caenorhabditis elegans Genotype: tat-5(tm1741) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT and GFP+. tm1741 homozygotes are small and sterile. Pick GFP+ heterozygotes to maintain. qIs48 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter driving GFP in the intestine, and is homozygous lethal. Reference: Wehman AM, et al. Curr Biol. 2011 Dec 6;21(23):1951-9. Mutagen: Outcrossed: x3 Made by: Ann Wehman Received: 02/01/12 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT2465 Species: C. elegans Genotype: xnSi85 I; hmg-5(xn168[hmg-5::gfp(11)] IV. Description: xnSi85 [mex-5p::mito(matrix)::GFP(1-10)::nos-2 3’UTR] I. Split GFP HMG-5/TFAM labels mtDNA nucleoids in primordial germ cells and germ line. Reference: Schwartz AZA, et al. eLife. 2022 Oct 6:11:e80396. doi: 10.7554/eLife.80396. PMID: 36200990. Mutagen: Outcrossed: x0 Made by: Aaron Schwartz Received: 04/10/24 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT250 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; xnIs96. Description: xnIs96 [pJN455(hmr-1p::hmr-1::GFP::unc-54 3'UTR) + unc-119(+)]. GFP starts in membranes at~100 cell stage, becomes bright apically in older embryos and larvae; most prominent in nerve ring in adults. Reference: Achilleos et al. (2010) Development 137(11):1833-42. Mutagen: Bombardment Outcrossed: x0 Made by: Ann Wehman Received: 04/28/10 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT36 Species: Caenorhabditis elegans Genotype: unc-101(m1) par-6(zu170) I; zuIs43. Description: zuIs43[pie-1::GFP::PAR-6::ZF1 + unc-119(+)]. Unc worms. GFP present in early embryos but then degrades in somatic lineages. Rescues Mel phenotype of par-6(zu170). Mutagen: Outcrossed: x Made by: Jeremy Nance Received: 04/05/07 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT404 Species: Caenorhabditis elegans Genotype: ect-2(gk44) II; unc-119(ed3) III; xnIs162. Description: xnIs162 [ect-2::GFP + unc-119(+)]. Transgene rescues sterility of ect-2(gk44). Reference: Chan E & Nance J. J Cell Sci. 2013 Apr 1;126(Pt 7):1692-702. Mutagen: Outcrossed: x Made by: Jeremy Nance Received: 07/24/13 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT422 Species: C. elegans Genotype: unc-119(ed3) III; xnIs178. Description: xnIs178 [mCherry::pac-1 + unc-119(+)]. Translational fusion to PAC-1. mCherry::PAC-1 is expressed in pharyngeal muscle cells (mostly apical, some basal), a few cells in the vulva, maternally in early embryos at cell contacts, and at adherens junctions in late embryogenesis. Maternal expression is difficult to detect by eye but visible with longer camera exposure. Reference: Anderson DC, et al. Science 2008: 320: 1771-1774. Mutagen: Outcrossed: x0 Made by: Jeremy Nance Received: 09/29/17 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT575 Species: C. elegans Genotype: unc-119(ed3); xnIs243. Description: xnIs243 [picc-1p::picc-1::GFP + unc-119(+)]. Weak maternal expression visible at early embryo cell contacts. Stronger zygotic expression visible at junctions of epithelial cells. Transgene generated from a recombineered genomic fosmid clone. Reference: Zilberman, Y., J. Abrams, D.C. Anderson, and J. Nance. 2017. Cdc42 regulates junctional actin but not cell polarization in the Caenorhabditis elegans epidermis. J Cell Biol 216: 3729-3744. Mutagen: Insertion made by biolistic transformati Outcrossed: x0 Made by: Dorian Anderson Received: 11/13/17 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT63 Species: Caenorhabditis elegans Genotype: xnIs17. Description: xnIs17 [dlg-1::GFP + rol-6(su1006)]. Rollers. Expresses DLG-1::GFP in epithelial cell junctions. Mutagen: UV/TMP Outcrossed: x2 Made by: Jeremy Nance Received: 11/20/07 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT741 Species: Caenorhabditis elegans Genotype: xnSi6 II; unc-119(ed3) III. Description: xnSi6 [mex-5p::hmr-1::GFP::hmr-1 3'UTR + unc-119(+)] II. HMR-1::GFP localized at cell contacts in early embryos and becomes enriched in PGCs. Reference: Chihara D & Nance J. Development. 2012 Jul;139(14):2547-56. Klompstra D, et al. Nat Cell Biol. 2015 Jun;17(6):726-35. Mutagen: Outcrossed: x0 Made by: Dorian Anderson Received: 08/17/15 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT778 Species: C. elegans Genotype: unc-119(ed3) III; xnIs299. Description: xnIs299 [hmr-1p::hmr-1::mCherry + unc-119(+)]. Translational fusion to hmr-1. HMR-1::mCherry is visible in multiple tissues including pharynx, intestine, epidermis and gonad; also marks the adherens junctions in epithelia. No visible maternal expression. Reference: Zilberman Y, et al. 2017: J Cell Biol. (In press). Mutagen: Outcrossed: x0 Made by: Jeremy Nance Received: 09/29/17 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT828 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; xnIs312. Description: xnIs312 [par-6p::par-6::mCherry + unc-119(+)]. Expresses par-6::mCherry maternally and zygotically. Expression is present in many cells, including early embryos, epithelial cells, the excretory cell, and the germ line. Reference: Armenti ST, Chan E, Nance J. Dev Biol. 2014 Aug 4. pii: S0012-1606(14)00355-8. Mutagen: Bombardment Outcrossed: x0 Made by: Emily Chan Received: 08/25/14 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT93 Species: Caenorhabditis elegans Genotype: pac-1(xn6) III. Description: Loss of radial polarity in early embryos. Maintain under normal conditions. Reference: Anderson DC, et al. Science. 2008 Jun 27;320(5884):1771-4. Mutagen: EMS Outcrossed: x6 Made by: Jeremy Nance Received: 01/24/12 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FT99 Species: Caenorhabditis elegans Genotype: pac-1(xn6) unc-119(ed3) III; xnIs27. Description: xnIs27 [pie-1p::GFP::pac-1 + unc-119(+)]. Maintain at 25C to slow silencing of transgene. xnIs27 rescues pac-1(xn6). GFP::PAC-1 localized at contacts in early embryos. Reference: Anderson DC, et al. Science. 2008 Jun 27;320(5884):1771-4. Klompstra D, et al. Nat Cell Biol. 2015 Jun;17(6):726-35. Mutagen: Outcrossed: x0 Made by: Dorian Anderson Received: 08/17/15 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: FX1053 Species: Caenorhabditis elegans Genotype: ins-11(tm1053) II. Description: Superficially wild type. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: UV/TMP Outcrossed: x5 Made by: Shohei Mitani Received: 07/16/07 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX11364 Species: Caenorhabditis elegans Genotype: pps-1(tm1109)/mec-3(u297) IV. Description: Heterozygotes are WT and segregate WT, Mec and larval lethals. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: UV/TMP Outcrossed: x1 Made by: S. Mitani Received: 04/23/07 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX1146 Species: Caenorhabditis elegans Genotype: sod-5(tm1146) II. Description: Homozygous viable. 775 bp deletion. 24926/24927 - 25701/25702. Primer 1: att gcc aat gcc gtt ctt cc (forward primer to the left of deletion). Primer 2: tat tat ttc gcg tcg gag cg (forward primer lies in the deletion). Primer 3: att tat gca gga gcg gca ag (reverse primer to the right of deletion). In sod-5(+) you see 720 bp product with primer 2 and 3. reliable PCR. in sod-5(+) expected product size is 1315 bp with primer 1 and 3. not always reliable. In sod-5 (tm1146) you see 540 bp product with primer 1 and 3. reliable PCR. In sod-5(tm1146), you see no product with primer 2 and 3. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: Outcrossed: x Made by: Shohei Mitani Received: 01/24/06 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: FX11501 Species: Caenorhabditis elegans Genotype: dpy-5(e61) uri-1(tm939)/dpy-5(e61) unc-14(e57) I. Description: Heterozygotes are Dpy. Segregate Dpy Unc. tm939 homozygotes are emb, leth, pvl, rup, larval arrested or sterile. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: Outcrossed: x2 Made by: Shohei Mitani Received: 05/22/07 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX11507 Species: Caenorhabditis elegans Genotype: rabs-5(tm2036) IV/nT1 [qIs51] (IV;V). Description: Heterozygotes are WT with pharyngeal GFP signal, and segregate arrested nT1 aneuploids, and non-GFP tm2036 homozygotes. tm2036 homozygotes are temperature sensitive lethal, and can be maintained as homozygotes at 15C. tm2036 homozygotes have endocytosis defects in oocytes and coelomocytes at 25C. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: UV/TMP Outcrossed: x4 Made by: Shohei Mitani Received: 07/16/07 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX11552 Species: Caenorhabditis elegans Genotype: vps-45(tm246)/egl-17(e1313) lon-2(e678) X. Description: Heterozygotes are WT and segregate WT, Egl Lon, and tm246 homozygotes (temperature sensitive lethal which can be maintained as homozygotes at 15C). tm246 homozygotes have endocytosis defects in oocytes and coelomocytes at 25C. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: UV/TMP Outcrossed: x4 Made by: Shohei Mitani Received: 07/16/07 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX1524 Species: Caenorhabditis elegans Genotype: cku-70(tm1524) III. Description: Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: Outcrossed: x0 Made by: S. Mitani Received: 12/28/12 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX17650 Species: C. elegans Genotype: lin-1(tm5929)/tmIn1 IV. Description: Homozygous lethal or sterile deletion allele balanced by Unc-marked translocation. Break points: In(egl-4 unc-17) IV. Covered region (Mb) 1.8 (1.8..3.6) Unc. Reference: Iwata S, et al. Sci Rep. 2016 Sep 21;6:33840. Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX17788 Species: C. elegans Genotype: mlt-7(tm1794)/tmIn4 II. Description: Heterozygotes are slightly Dpy, and segregate slightly Dpy mlt-7/tmIn4 heterozygotes, Dpy tmIn4 homozygotes, and mlt-7(tm1794) homozygotes (Let). Break points: In(lin-8 dpy-2) II. Covered region (Mb) 3.7 (3.1..6.7) Dpy. Reference: Iwata S, et al. Sci Rep. 2016 Sep 21;6:33840. Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Shohei Mitani Received: 06/30/17 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX19059 Species: C. elegans Genotype: Y38F2AR.9(tm1986)/tmIn3 IV. Description: Homozygous lethal deletion allele balanced by Unc-marked translocation. Break points: In(jtr-1 unc-17) IV. Covered region (Mb) 2.2 (1.4..3.6) Unc. Reference: Iwata S, et al. Sci Rep. 2016 Sep 21;6:33840. Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX19119 Species: C. elegans Genotype: atm-1(tm5027) C46H11.6(tm7811) I; tmIn20 II; xpc-1(tm3886) F01D4.9(tm7812) IV; aqp-4(tm7813) V. Description: tmIn20 is an inversion between F46C5.9 and C08H9.13 in LG II.  Inversion strain obtained by Next-generation sequencing. Mutagen: TMP/UV Outcrossed: x0 Made by: Shohei Mitani Received: 06/30/17 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX19120 Species: C. elegans Genotype: atm-1(tm5027) Y47G6A.28(tm7889) nepr-1(tm7890) I; C18H9.6(tm7891) II; xpc-1(tm3886) tmIn21 IV; srh-54(tm7892) V. Description: tmIn21 is an inversion between pck-3 and R09H10.5 in LG IV.  Inversion strain obtained by Next-generation sequencing. Mutagen: TMP/UV Outcrossed: xo Made by: Shohei Mitani Received: 06/30/17 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX19134 Species: C. elegans Genotype: tmIn8 II. Description: Break points: In(F13D12.6 Y51H1A.2) II. Covered region (Mb) 2.1 (11.7..13.9) Obtained by TMP/UV. (Note: Y51H1A.2 has been renamed cup-14.) [NOTE: the genotype originally listed for this strain in Table 2 of Dejima, et al. was incorrect.] Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Shohei Mitani Received: 06/30/17 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX19141 Species: C. elegans Genotype: atm-1(tm5027) C37A2.6(tm8115) I; Y48A6B.8(tm8116) III; xpc-1(tm3886) IV; tmIn22 V. Description: tmIn22 is an inversion between W02G9.9 and 21ur-15544 in LG V.  Inversion strain obtained by Next-generation sequencing. Mutagen: TMP/UV Outcrossed: x0 Made by: Shohei Mitani Received: 06/30/17 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX19161 Species: C. elegans Genotype: dpy-20(tm5940)/tmIn5 IV. Description: Homozygous lethal or sterile deletion allele balanced by Unc-marked translocation. Break points: In(mec-3 unc-31) IV. Covered region (Mb) 2.3 (10.5..12.8) Unc. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX19163 Species: C. elegans Genotype: mca-3(tm6395)/tmIn11 IV. Description: Homozygous lethal deletion allele balanced by Unc-marked translocation. Heterozygotes are wild-type and segregate wild-type heterozygotes, lethal tm6395 homozygotes, and Unc tmIn11 homozygotes. Break points: In(kvs-5 unc-17) IV. Covered region (Mb) 2.9 (0.7..3.6) [NOTE: the genotype originally listed for this strain in Table 2 of Dejima, et al. was incorrect.] Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX19170 Species: C. elegans Genotype: lin-1(tm5929)/tmIn2 IV. Description: Homozygous lethal or sterile deletion allele balanced by Unc-marked translocation. Break points: In(ced-2 unc-17) IV. Covered region (Mb) 2 (1.6..3.6) Unc. Reference: Iwata S, et al. Sci Rep. 2016 Sep 21;6:33840. Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX19171 Species: C. elegans Genotype: lig-4(tm750) III; tmIn26 X. Description: tmIn26 homozygotes are Lon and Mec. Break points: In(lon-2 mec-10) X. Covered region (Mb) 3.7 (4.7..8.5) Lon Mec. [NOTE: the genotype originally listed for this strain in Table 2 of Dejima, et al. was incorrect.] Reference: Iwata S, et al. Sci Rep. 2016 Sep 21;6:33840. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Shohei Mitani Received: 06/30/17 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX19173 Species: C. elegans Genotype: lig-4(tm750) III; tmIn19 V. Description: Break points: In(unc-23 lon-3) V. Covered region (Mb) 3.3 (8.9..12.2) Lon Unc. [NOTE: the genotype originally listed for this strain in Table 2 of Dejima, et al. was incorrect.] Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX19181 Species: C. elegans Genotype: unc-15(tm6329)/tmIn14 I. Description: Homozygous lethal deletion allele balanced by Dpy-marked translocation. Break points: In(dpy-5 lin-10) I. Covered region (Mb) 2.7 (5.4..8.1). Pick wild-type to maintain. Heterozygotes are wild-type and segregate wild-type heterozygotes, Dpy (tmIn14 homozygotes), and unc-15 homozygotes. [NOTE: the genotype originally listed for this strain in Table 2 of Dejima, et al. was incorrect.] Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX19397 Species: C. elegans Genotype: tmC1 X; tmEx4487. Description: tmEx4487 [unc-18(+) + myo-2p::Venus]. Break points: In(F53B1.2 unc-18 In(lon-2 mec-10)) X. Covered region (Mb) 6.4 (2.1..8.5) Lon Mec (Unc). Pick fluorescent non-Unc to maintain array. Males carrying the array (Venus in pharynx) can mate. Reference: Iwata S, et al. Sci Rep. 2016 Sep 21;6:33840. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX19472 Species: C. elegans Genotype: tmIn10 II. Description: Break points: In(ZK1240.1 F29A7.8) II. Covered region (Mb) 0.4 (2.3..2.8) Obtained by TMP/UV. [NOTE: the genotype originally listed for this strain in Table 2 of Dejima, et al. was incorrect.] Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX19584 Species: C. elegans Genotype: lig-4(tm750) III; tmIn51 IV. Description: tmIn51 is a CRISPR/Cas9-induced inversion between C09G12.5 and C01B10.3 in LG IV. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Shohei Mitani Received: 06/30/17 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX19585 Species: C. elegans Genotype: lig-4(tm750) tmIn52 III. Description: Break points: In(hpr-9 ttr-52) III. Covered region (Mb) 2.8 (10.6..13.4) [NOTE: the genotype originally listed for this strain in Table 2 of Dejima, et al. was incorrect.] Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX19666 Species: C. elegans Genotype: tmC5 IV. Description: Break points: In(C01B10.3 eak-7 In(mec-3 unc-31)) IV. Covered region (Mb) 6.2 (6.6..12.8) Mec Unc. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX19668 Species: C. elegans Genotype: tmC6 [dpy-2(tmIs1189)] II. Description: Break points: In(sri-57 asm-1 In(ZK1240.1 F29A7.8)) II. Covered region (Mb) 4.6 (2.3..6.9) Balancer marked with myo-2p::Venus. Dpy. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX19702 Species: C. elegans Genotype: lig-4(tm750) III; tmIn54 V. Description: Break points: In(srbc-66 T10H9.8) V. Covered region (Mb) 3.1 (3.5..6.7) [NOTE: the genotype originally listed for this strain in Table 2 of Dejima, et al. was incorrect.] Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: CRISPR/Cas Outcrossed: x0 Made by: Shohei Mitani Received: 06/30/17 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX19704 Species: C. elegans Genotype: tmIn58 I; lig-4(tm750) III. Description: Break points: In(gsp-3 sre-23) I. Covered region (Mb) 3.5 (4.7..8.3) [NOTE: the genotype originally listed for this strain in Table 2 of Dejima, et al. was incorrect.] Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Shohei Mitani Received: 06/30/17 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX19706 Species: C. elegans Genotype: lig-4(tm750) III; tmIn60 X. Description: Break points: In(odr-7 F59F4.2) X. Covered region (Mb) 3.4 (12.5..15.8) Unknown if tmIn60 homozygotes are Odr. [NOTE: the genotype originally listed for this strain in Table 2 of Dejima, et al. was incorrect.] Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Shohei Mitani Received: 06/30/17 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX19992 Species: C. elegans Genotype: lig-4(tm750) III; tmIn62 IV. Description: Break points: In(kvs-5 dmd-9) IV. Covered region (Mb) 2.5 (0.7..3.3) [NOTE: the genotype originally listed for this strain in Table 2 of Dejima, et al. was incorrect.] Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX2066 Species: Caenorhabditis elegans Genotype: his-72(tm2066) III. Description: Homozygous viable and fertile. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: Outcrossed: x6 Made by: Shohei Mitani Received: 05/04/07 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: FX2355 Species: Caenorhabditis elegans Genotype: ift-81(tm2355) X. Description: Superficially WT. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: UV/TMP Outcrossed: x2 Made by: Shohei Mitani Received: 07/23/07 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX2394 Species: Caenorhabditis elegans Genotype: ift-74(tm2394) II. Description: Superficially WT. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: UV/TMP Outcrossed: x2 Made by: Shohei Mitani Received: 07/23/07 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX291 Species: Caenorhabditis elegans Genotype: +/eT1 III; spn-4(tm291)/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. 1/3 of WT animals (spn-4 homozygotes) are Mels: segregate only dead embryos with have no morphogenesis. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: UV/TMP Outcrossed: x Made by: Shohei Mitani Received: 05/13/03 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX2956 Species: Caenorhabditis elegans Genotype: mps-4(tm2596) III. Description: Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: UV/TMP Outcrossed: x0 Made by: Shohei Mitani Received: 08/03/07 University of Medicine & Dentistry of New Jersey, Piscataway, NJ ----------------------------------------------------------------------------- Strain: FX301 Species: Caenorhabditis elegans Genotype: gsp-2(tm301) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Maintain by picking wild-type GFP+. Heterozygotes are WT GFP+ and should segregate WT GFP+ heterozygotes, non-GFP gsp-2 homozygotes (embryonic lethal), very rare GFP+ homozygous hT2, and dead eggs. hT2[qIs48] animals are recessive lethal. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: Outcrossed: x2 Made by: Shohei Mitani Received: 06/20/05 Center for Biomedical Research and Education, School of Medicine, Kanazawa University ----------------------------------------------------------------------------- Strain: FX30123 Species: C. elegans Genotype: tmC24 [F23D12.4(tmIs1233)] X. Description: Break points: In(mec-10 Y7A5A.20 In(odr-7 F59F4.2)) X. Covered region (Mb) 7.4 (8.5..15.8) Balancer marked with myo-2p::mCherry. Mec. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30126 Species: C. elegans Genotype: tmC1 X. Description: Break points: In(F53B1.2 unc-18 In(lon-2 mec-10)) X. Covered region (Mb) 6.4 (2.1..8.5) Lon Unc Mec. Reference: Iwata S, et al. Sci Rep. 2016 Sep 21;6:33840. Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30133 Species: C. elegans Genotype: tmC3 V. Description: Break points: In(unc-83 C27A7.1 In(unc-23 lon-3)) V. Covered region (Mb) 5.7 (6.5..12.2) Lon Unc. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30134 Species: C. elegans Genotype: tmC3 [egl-9(tmIs1228)] V. Description: Break points: In(unc-83 C27A7.1 In(unc-23 lon-3)) V. Covered region (Mb) 5.7 (6.5..12.2) Balancer marked with myo-2p::Venus. Lon Unc. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30135 Species: C. elegans Genotype: tmC3 [egl-9(tmIs1230)] V. Description: Break points: In(unc-83 C27A7.1 In(unc-23 lon-3)) V. Covered region (Mb) 5.7 (6.5..12.2) Balancer marked with myo-2p::mCherry. Lon Unc. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30138 Species: C. elegans Genotype: tmC6 [dpy-2(tmIs1208)] II. Description: Break points: In(sri-57 asm-1 In(ZK1240.1 F29A7.8)) II. Covered region (Mb) 4.6 (2.3..6.9) Balancer marked with myo-2p::mCherry. Dpy. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30140 Species: C. elegans Genotype: tmC5 [F36H1.3(tmIs1220)] IV. Description: Break points: In(C01B10.3 eak-7 In(mec-3 unc-31)) IV. Covered region (Mb) 6.2 (6.6..12.8) Balancer marked with myo-2p::Venus. Mec Unc. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30143 Species: C. elegans Genotype: tmC6 II. Description: Break points: In(sri-57 asm-1 In(ZK1240.1 F29A7.8)) II. Covered region (Mb) 4.6 (2.3..6.9) Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30144 Species: C. elegans Genotype: tmC6 [dpy-2(tm9710)] II. Description: Break points: In(sri-57 asm-1 In(ZK1240.1 F29A7.8)) II. Covered region (Mb) 4.6 (2.3..6.9) Dpy. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30151 Species: C. elegans Genotype: tmC9 IV. Description: Break points: In(glb-19 lgc-52 In(mec-3 unc-31)) IV. Covered region (Mb) 4.8 (10.5..15.2) Unc Mec. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30152 Species: C. elegans Genotype: tmC12 [egl-9(tmIs1194)] V. Description: Break points: In(hlh-10 C01G10.10 In(unc-23 lon-3)) V. Covered region (Mb) 6.1 (8.9..15.1) Balancer marked with myo-2p::Venus. Lon Unc. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30153 Species: C. elegans Genotype: tmC12 [egl-9(tmIs1197)] V. Description: Break points: In(hlh-10 C01G10.10 In(unc-23 lon-3)) V. Covered region (Mb) 6.1 (8.9..15.1) Balancer marked with myo-2p::mCherry. Lon Unc. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30154 Species: C. elegans Genotype: tmC12 V. Description: Break points: In(hlh-10 C01G10.10 In(unc-23 lon-3)) V. Covered region (Mb) 6.1 (8.9..15.1) Lon Unc. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30161 Species: C. elegans Genotype: tmC16 [unc-60(tmIs1237)] V. Description: Break points: In(flp-34 C04E6.7 In(srbc-66 T10H9.8)) V. Covered region (Mb) 5.6 (1..6.7) Balancer marked with myo-2p::mCherry. Unc. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30162 Species: C. elegans Genotype: tmC16 V. Description: Break points: In(flp-34 C04E6.7 In(srbc-66 T10H9.8)) V. Covered region (Mb) 5.6 (1..6.7) Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30164 Species: C. elegans Genotype: tmC16 [unc-60(tm9712)] V. Description: Break points: In(flp-34 C04E6.7 In(srbc-66 T10H9.8)) V. Covered region (Mb) 5.6 (1..6.7) Unc. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30166 Species: C. elegans Genotype: tmC18 I. Description: Break points: In(B0207.10 dnj-27 In(gsp-3 sre-23)) I. Covered region (Mb) 7.2 (4.7..11.9) Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30167 Species: C. elegans Genotype: tmC18 [dpy-5(tmIs1200)] I. Description: Break points: In(B0207.10 dnj-27 In(gsp-3 sre-23)) I. Covered region (Mb) 7.2 (4.7..11.9) Balancer marked with myo-2p::Venus. Dpy. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30168 Species: C. elegans Genotype: tmC18 [dpy-5(tmIs1236)] I. Description: Break points: In(B0207.10 dnj-27 In(gsp-3 sre-23)) I. Covered region (Mb) 7.2 (4.7..11.9) Balancer marked with myo-2p::mCherry. Dpy. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30176 Species: C. elegans Genotype: tmC20 I. Description: Break points: In(F53G12.8 T02E1.7 In(gsp-3 sre-23)) I. Covered region (Mb) 8.1 (0.1..8.3) Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30177 Species: C. elegans Genotype: tmC20 [unc-14(tmIs1219)] I. Description: Break points: In(F53G12.8 T02E1.7 In(gsp-3 sre-23)) I. Covered region (Mb) 8.1 (0.1..8.3) Balancer marked with myo-2p::Venus. tmIs1219 is inserted in unc-14, but Unc phenotype is not detectable. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30179 Species: C. elegans Genotype: tmC20 [unc-14(tmIs1219) dpy-5(tm9715)] I. Description: Break points: In(F53G12.8 T02E1.7 In(gsp-3 sre-23)) I. Covered region (Mb) 8.1 (0.1..8.3) Balancer marked with myo-2p::Venus. Dpy. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30185 Species: C. elegans Genotype: tmC24 X. Description: Break points: In(mec-10 Y7A5A.20 In(odr-7 F59F4.2)) X. Covered region (Mb) 7.4 (8.5..15.8) Mec. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30186 Species: C. elegans Genotype: tmC24 [F23D12.4(tmIs1233) unc-9(tm9718)] X. Description: Break points: In(mec-10 Y7A5A.20 In(odr-7 F59F4.2)) X. Covered region (Mb) 7.4 (8.5..15.8) Balancer marked with myo-2p::mCherry. Unc Mec. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30194 Species: C. elegans Genotype: tmC24 [F23D12.4(tmIs1240) unc-9(tm9719)] X. Description: Break points: In(mec-10 Y7A5A.20 In(odr-7 F59F4.2)) X. Covered region (Mb) 7.4 (8.5..15.8) Balancer marked with myo-2p::Venus. Unc Mec. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30197 Species: C. elegans Genotype: tmC25 IV. Description: Break points: In(mak-2 unc-8 In(kvs-5 dmd-9)) IV. Covered region (Mb) 6.5 (0.7..7.2) One breakpoint is in unc-8, but Unc phenotype is not detectable. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30203 Species: C. elegans Genotype: tmC25 [unc-5(tmIs1241)] IV. Description: Break points: In(mak-2 unc-8 In(kvs-5 dmd-9)) IV. Covered region (Mb) 6.5 (0.7..7.2) Balancer marked with myo-2p::Venus. Unc. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30205 Species: C. elegans Genotype: tmC27 I. Description: Break points: In(ile-1 Y18D10A.2 In(dnj-27 dkf-1)) I. Covered region (Mb) 4 (9.6..13.6) Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30208 Species: C. elegans Genotype: tmC27 [unc-75(tmIs1239)] I. Description: Break points: In(ile-1 Y18D10A.2 In(dnj-27 dkf-1)) I. Covered region (Mb) 4 (9.6..13.6) Balancer marked with myo-2p::Venus. Unc. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30218 Species: C. elegans Genotype: tmC30 [ubc-17(tmIs1247)] X. Description: Break points: In(Y102A11A.6 R09F10.1 In(lon-2 mec-10)) X. Covered region (Mb) 6.4 (2..8.5) Balancer marked with myo-2p::Venus. Lon Mec. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30225 Species: C. elegans Genotype: tmIn65 I; lig-4(tm750) III. Description: Break points: In(dnj-27 dkf-1) I. Covered region (Mb) 1.8 (11.9..13.6) [NOTE: the genotype originally listed for this strain in Table 2 of Dejima, et al. was incorrect.] Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30229 Species: C. elegans Genotype: tmC30 X. Description: Break points: In(Y102A11A.6 R09F10.1 In(lon-2 mec-10)) X. Covered region (Mb) 6.4 (2..8.5) Lon Mec. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30233 Species: C. elegans Genotype: tmC16 [unc-60(tmIs1210)] V. Description: Break points: In(flp-34 C04E6.7 In(srbc-66 T10H9.8)) V. Covered region (Mb) 5.6 (1..6.7) Balancer marked with myo-2p::Venus. Unc. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30234 Species: C. elegans Genotype: tmC9 [F36H1.2(tmIs1221)] IV. Description: Break points: In(glb-19 lgc-52 In(mec-3 unc-31)) IV. Covered region (Mb) 4.8 (10.5..15.2) Balancer marked with myo-2p::Venus. Unc Mec. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30235 Species: C. elegans Genotype: tmC20 [dpy-5(tm9709)] I. Description: Break points: In(F53G12.8 T02E1.7 In(gsp-3 sre-23)) I. Covered region (Mb) 8.1 (0.1..8.3) Dpy. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30236 Species: C. elegans Genotype: tmC30 [ubc-17(tmIs1243)] X. Description: Break points: In(Y102A11A.6 R09F10.1 In(lon-2 mec-10)) X. Covered region (Mb) 6.4 (2..8.5) Balancer marked with myo-2p::mCherry. Lon Mec. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30237 Species: C. elegans Genotype: tmC24 [unc-9(tm9723)] X. Description: Break points: In(mec-10 Y7A5A.20 In(odr-7 F59F4.2)) X. Covered region (Mb) 7.4 (8.5..15.8) Unc Mec. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30238 Species: C. elegans Genotype: tmC18 [dpy-5(tm9705)] I. Description: Break points: In(B0207.10 dnj-27 In(gsp-3 sre-23)) I. Covered region (Mb) 7.2 (4.7..11.9) Dpy. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30240 Species: C. elegans Genotype: tmC24 [F23D12.4(tmIs1240)] X. Description: Break points: In(mec-10 Y7A5A.20 In(odr-7 F59F4.2)) X. Covered region (Mb) 7.4 (8.5..15.8) Balancer marked with myo-2p::Venus. Mec. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30252 Species: C. elegans Genotype: tmC24 [F23D12.4(tmIs1240) unc-9(tm9719)] X; tmEx4950. Description: tmIs1240 [myo-2p::Venus, X: F23D12.4] X. tmEx4950 [unc-9(+) + vha-6p::GFP]. Pick non-Unc with bright GFP+ in gut to maintain array. Balancer marked with myo-2p::Venus. Mec (Unc). Balancer break points: In(mec-10 Y7A5A.20 In(odr-7 F59F4.2)) X. Covered region (Mb) 7.4 (8.5..15.8) Males carrying the array (intestinal GFP) can mate. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30253 Species: C. elegans Genotype: tmC24 [F23D12.4(tmIs1233) unc-9(tm9718)] X; tmEx4950. Description: tmIs1233 [myo-2p::mCherry, X: F23D12.4] X. tmEx4950 [unc-9(+) + vha-6p::GFP]. Pick non-Unc with bright GFP+ in gut to maintain array. Balancer marked with myo-2p::mCherry. Mec (Unc). Balancer break points: In(mec-10 Y7A5A.20 In(odr-7 F59F4.2)) X. Covered region (Mb) 7.4 (8.5..15.8) Males carrying the array (intestinal GFP) can mate. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30257 Species: C. elegans Genotype: tmC25 [unc-5(tm9708)] IV. Description: Break points: In(mak-2 unc-8 In(kvs-5 dmd-9)) IV. Covered region (Mb) 6.5 (0.7..7.2) Unc. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30258 Species: C. elegans Genotype: tmC27 [unc-75(tm9711)] I. Description: Break points: In(ile-1 Y18D10A.2 In(dnj-27 dkf-1)) I. Covered region (Mb) 4 (9.6..13.6) Unc. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30262 Species: C. elegans Genotype: lin-42(tmIs1246) II. Description: Break points: lin-42 II. Covered region (Mb) (1.2) Balancer marked with myo-2p::Venus. Egl. [NOTE: the genotype originally listed for this strain in Table 2 of Dejima, et al. was incorrect.] Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30266 Species: C. elegans Genotype: lin-42(tmIs1226) II. Description: Break points: lin-42 II. Covered region (Mb) (1.2) Balancer marked with myo-2p::mCherry. tmIs1226 is integrated in the same site as tmIs1246, but Egl phenotype is not detectable. [NOTE: the genotype originally listed for this strain in Table 2 of Dejima, et al. was incorrect.] Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30269 Species: C. elegans Genotype: dpy-9(tm9713) kvs-5(tmIs1245) IV. Description: Break points: dpy-9 kvs-5 IV. Covered region (Mb) (0.3..0.7) Balancer marked with myo-2p::Venus. Dpy. [NOTE: the genotype originally listed for this strain in Table 2 of Dejima, et al. was incorrect.] Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30273 Species: C. elegans Genotype: egl-17(tmIs1224) X. Description: Break points: egl-17 X. Covered region (Mb) (0.5) Balancer marked with myo-2p::Venus. Egl. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX30276 Species: C. elegans Genotype: egl-17(tmIs1234) X. Description: Break points: egl-17 X. Covered region (Mb) (0.5) Balancer marked with myo-2p::mCherry. Egl. Reference: Dejima K, et al. Cell Rep. 2018 Jan 2;22(1):232-241. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Mitani Lab Received: 03/08/18 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX536 Species: Caenorhabditis elegans Genotype: ced-13(tm536) X. Description: Deletion allele. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: Outcrossed: x Made by: Shohei Mitani Received: 03/25/05 The Rockefeller University, New York NY, USA ----------------------------------------------------------------------------- Strain: FX544 Species: Caenorhabditis elegans Genotype: cdc-48.1(tm544) II. Description: Received new stock 10/30/08. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: Outcrossed: x0 Made by: Shohei Mitani Received: 07/15/06 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX546 Species: Caenorhabditis elegans Genotype: sqv-5(tm546)/unc-55(e402) I. Description: T24D1.1 Heterozygotes are wild-type and segregate wild-type heterozygotes, unc-55 homozygotes, and sqv-5 homozygotes (sterile, clear, sickly). Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: UV/TMP Outcrossed: x2 Made by: Shohei Mitani Received: 07/24/03 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX627 Species: Caenorhabditis elegans Genotype: lgc-11(tm627) X. Description: No obvious phenotype. 446 bp deletion. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: UV/TMP Outcrossed: x0 Made by: S Mitani Received: 04/30/07 Universite Claude Bernard Lyon 1, Lyon, France ----------------------------------------------------------------------------- Strain: FX659 Species: Caenorhabditis elegans Genotype: cdc-48.2(tm659) II. Description: Received new stock 10/30/08. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: Outcrossed: x0 Made by: Shohei Mitani Received: 07/15/06 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX683 Species: Caenorhabditis elegans Genotype: spas-1(tm683) V. Description: Superficially WT. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: UV/TMP Outcrossed: x0 Made by: Shohei Mitani Received: 08/03/07 Tokyo Women's Medical University School of Medicine, Tokyo, Japan ----------------------------------------------------------------------------- Strain: FX776 Species: Caenorhabditis elegans Genotype: sod-1(tm776) II. Description: Homozygous viable. 612 bp deletion. 17154/17155 - 17766/17767. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: Outcrossed: x Made by: Shohei Mitani Received: 01/24/06 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: FX794 Species: Caenorhabditis elegans Genotype: rnf-5(tm794) III. Description: Homozygous viable. Received from Limor Broday. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: UV/TMP Outcrossed: x4 Made by: S. Mitani Received: 11/04/04 Technion Research and Development Foundation, Haifa, Israel ----------------------------------------------------------------------------- Strain: FX863 Species: Caenorhabditis elegans Genotype: acr-7(tm863) II. Description: No obvious phenotype. 625 bp deletion + 7 bp insertion. Attribution: This strain was generated by the National Bioresource Project at the Tokyo Women's Medical University School of Medicine, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807 Mutagen: UV/TMP Outcrossed: x0 Made by: S Mitani Received: 04/30/07 Universite Claude Bernard Lyon 1, Lyon, France ----------------------------------------------------------------------------- Strain: FZ282 Species: Caenorhabditis elegans Genotype: sec-5(pk2357)/dpy-10(e128) II. Description: Heterozygotes segregate wild-type heterozygotes, Dpy, and sec-5 homozygotes (scrawny, small broods, abnormal gut appearance) sec-5 is homozygous maternal-effect lethal; M+Z- animals produce a few dead L1-L2 stage larvae with Vab defects. Based upon phenotype, pk2357 is a strong loss-of-function, but likely not a null allele; molecular lesion produces a premature stop at position 389. Reference: Frische EW, et al. EMBO J. 2007 Dec 12;26(24):5083-92. [NOTE: This strain was previously described as carrying pk2358, but pk2357 is the correct allele. Both pk2357 and pk2358 cause the same nonsense (amber) change.] Mutagen: Outcrossed: x Made by: Fried Zwartkruis Received: 04/16/12 Texas A&M Health Science Center, Houston TX ----------------------------------------------------------------------------- Strain: GA132 Species: Caenorhabditis elegans Genotype: daf-16(mgDf50) I; daf-2(m646) III. Description: Maintain at 15C. Mutagen: Outcrossed: x Made by: M. Nanji Received: 03/24/09 University College, London, England ----------------------------------------------------------------------------- Strain: GA158 Species: Caenorhabditis elegans Genotype: daf-16(mgDf50) I; daf-2(m65) III. Description: Maintain at 15C. Mutagen: Outcrossed: x Made by: M. Nanji Received: 03/24/09 University College, London, England ----------------------------------------------------------------------------- Strain: GA184 Species: Caenorhabditis elegans Genotype: sod-2(gk257) I. Description: Slow growing. Reduced brood size. Hypersensitive to oxidative stress. Mutagen: Outcrossed: x4 Made by: Josh McElwee Received: 11/13/09 University College, London, England ----------------------------------------------------------------------------- Strain: GA186 Species: Caenorhabditis elegans Genotype: sod-3(tm760) X. Description: Superficially wild-type. Mutagen: Outcrossed: x4 Made by: Josh McElwee Received: 11/13/09 University College, London, England ----------------------------------------------------------------------------- Strain: GA187 Species: Caenorhabditis elegans Genotype: sod-1(tm776) II. Description: Superficially wild-type. Mutagen: Outcrossed: x3 Made by: James Stephenson Received: 11/13/09 University College, London, England ----------------------------------------------------------------------------- Strain: GA2001 Species: Caenorhabditis elegans Genotype: wuIs305. Description: wuIs305 [myo-3p::Queen-2m]. ATP sensor Queen-2m is expressed under the control of myo-3 promoter. Reference: Galimov et al. Cell Rep. 2018 Mar 6;22(10):2730-2741. Mutagen: Outcrossed: x6 Made by: Evgeny Galimov Received: 04/30/18 University College, London, England ----------------------------------------------------------------------------- Strain: GA2002 Species: Caenorhabditis elegans Genotype: daf-2(e1370) III; wuIs305. Description: wuIs305 [myo-3p::Queen-2m]. Temperature sensitive dauer constitutive. Maintain at 15C. ATP sensor Queen-2m is expressed under the control of myo-3 promoter. Reference: Galimov et al. Cell Rep. 2018 Mar 6;22(10):2730-2741. Mutagen: Outcrossed: x6 Made by: Evgeny Galimov Received: 04/30/18 University College, London, England ----------------------------------------------------------------------------- Strain: GA2003 Species: Caenorhabditis elegans Genotype: daf-16(mgDf50) I; daf-2(e1370) III; wuIs305. Description: wuIs305 [myo-3p::Queen-2m]. ATP sensor Queen-2m is expressed under the control of myo-3 promoter. Reference: Galimov et al. Cell Rep. 2018 Mar 6;22(10):2730-2741. Mutagen: Outcrossed: x6 Made by: Evgeny Galimov Received: 04/30/18 University College, London, England ----------------------------------------------------------------------------- Strain: GA416 Species: Caenorhabditis elegans Genotype: sod-4(gk101) III. Description: Superficially wild-type. Mutagen: Outcrossed: x6 Made by: Josh McElwee Received: 11/13/09 University College, London, England ----------------------------------------------------------------------------- Strain: GA468 Species: Caenorhabditis elegans Genotype: geIs3 I. Description: geIs3 [sir-2.1(+) + rol-6(su1006)]. Rollers. This strain replaces LG100. Reference: Burnett C, et al. Nature. 2011 Sep 21;477(7365):482-5. Mutagen: Outcrossed: x5 Made by: Josh McElwee Received: 10/20/11 University College, London, England ----------------------------------------------------------------------------- Strain: GA480 Species: Caenorhabditis elegans Genotype: sod-2(gk257) I; sod-3(tm760) X. Description: Slow growing. Reduced brood size. Hypersensitive to oxidative stress. Mutagen: Outcrossed: x Made by: Josh McElwee Received: 11/13/09 University College, London, England ----------------------------------------------------------------------------- Strain: GA503 Species: Caenorhabditis elegans Genotype: sod-5(tm1146) II. Description: Superficially wild-type. Mutagen: Outcrossed: x7 Made by: Glenda Walker Received: 11/13/09 University College, London, England ----------------------------------------------------------------------------- Strain: GA631 Species: C. elegans Genotype: lin-15B&lin-15A(n765) X; wuIs177. Description: wuIs177 [ftn-1p::GFP + lin-15(+)]. GFP expression in the intestine. ftn-1p::GFP transgene shows moderate basal expression under standard culture conditions in an otherwise wild-type background, but is strongly induced by reduced insulin/IGF-1 signalling, reduced HIF signalling, and increased free iron levels. References: Ackerman D & Gems D. PLoS Genet. 2012;8(3):e1002498. Valentini S, et al. Mech Ageing Dev. 2012 May;133(5):282-90. Mutagen: Outcrossed: x6 Made by: Daniel Ackerman Received: 02/12/19 University College, London, England ----------------------------------------------------------------------------- Strain: GA800 Species: Caenorhabditis elegans Genotype: wuIs151. Description: wuIs151 [ctl-1(+) + ctl-2(+) + ctl-3(+) + myo-2p::GFP].  wuIs151 contains multiple copies of a PCR fragment containing the entire ctl-1 ctl-2 ctl-3 gene cluster.  Slow growing, especially at 25 degrees.  Raise at 20 degress or cooler. Mutagen: N/A Outcrossed: x6 Made by: R Doonan / G Walker Received: 11/13/09 University College, London, England ----------------------------------------------------------------------------- Strain: GA801 Species: Caenorhabditis elegans Genotype: wuIs152. Description: wuIs152 contains [sod-1(genomic) + rol-6(su1006)]. Rolls. Mutagen: X-ray Outcrossed: x4 Made by: R Doonan / D Gems Received: 11/13/09 University College, London, England ----------------------------------------------------------------------------- Strain: GA805 Species: Caenorhabditis elegans Genotype: wuIs156. Description: wuIs156 contains [sod-2(genomic) + rol-6(su1006)]. Rollers. Mutagen: X-ray Outcrossed: x6 Made by: R Doonan / D Gems Received: 11/13/09 University College, London, England ----------------------------------------------------------------------------- Strain: GA905 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; pkIs1642. Description: pkIs1642 [sir-2.1(+) + unc-119(+)]. Derived by outcrossing NL3908 to HT1593 (six times). Reference: Burnett C, et al. Nature. 2011 Sep 21;477(7365):482-5. Mutagen: Outcrossed: x6 Made by: Sara Valentini Received: 10/20/11 University College, London, England ----------------------------------------------------------------------------- Strain: GA906 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; pkIs1641. Description: pkIs1641 [unc-119(+)]. Derived by outcrossing NL3908 to HT1593 (six times). Reference: Burnett C, et al. Nature. 2011 Sep 21;477(7365):482-5. Mutagen: Outcrossed: x6 Made by: Sara Valentini Received: 10/20/11 University College, London, England ----------------------------------------------------------------------------- Strain: GA916 Species: Caenorhabditis elegans Genotype: dyf-x(wu250) I. Description: Dyf. Derived by crossing dye-filling mutant wu250 away from geIs3 (LG100 x N2 males). Reference: Burnett C, et al. Nature. 2011 Sep 21;477(7365):482-5. Mutagen: Outcrossed: x1 Made by: Sara Valentini Received: 10/20/11 University College, London, England ----------------------------------------------------------------------------- Strain: GB244 Species: Caenorhabditis elegans Genotype: unc-96(sf18) X. Description: Adults are Unc - reduced motility; characteristic birefrigent "needles" in body wall muscle cells by polarized light microscopy; contain accumulations of paramyosin and UNC-98. Phenotype (needles and accumulations of paramyosin) is suppressed by growth at 15C or by starvation. Mutagen: EMS Outcrossed: x3 Made by: K Mercer/G Benian Received: 11/25/08 Emory University, Atlanta, GA ----------------------------------------------------------------------------- Strain: GB246 Species: Caenorhabditis elegans Genotype: unc-98(sf19) X. Description: Adults are Unc - reduced motility; characteristic birefrigent "needles" in body wall muscle cells by polarized light microscopy; contain accumulations of paramyosin and UNC-96. Mutagen: ENU Outcrossed: x3 Made by: K Mercer/G Benian Received: 11/25/08 Emory University, Atlanta, GA ----------------------------------------------------------------------------- Strain: GB247 Species: Caenorhabditis elegans Genotype: unc-94(sf20) I. Description: Adults are Unc - reduced motility; disorganization of myofilament lattice in body wall muscle cells by polarized light microscopy; abnormal accululations of F-actin near muscle cell-cell boundaries. Mutagen: EMS Outcrossed: x6 Made by: K Mercer/G Benian Received: 11/25/08 Emory University, Atlanta, GA ----------------------------------------------------------------------------- Strain: GC363 Species: Escherichia coli Genotype: E. coli. Description: Bacteria. E. coli HT115(DE3) bacterial strain carrying pGC8. pGC8 is a partial cDNA of him-14 (ZK1127.11) cloned into the Timmons and Fire "double T-7 vector" L4440. The source of the cDNA is Yuji Kohara's clone yk240h12. pGC8 was constructed by inserting the 1.65kb KpnI/SacI fragment of the him-14 cDNA (from base pair 1071 to 192 base pairs beyond the stop codon) into the same sites in L4440. HT115(DE3) carrying pGC8 should be selected in the presence of 50 um/ml tetracyline and 100 um/ml ampicillin. Prior to an actual feeding experiment, it can be grown in liquid in the presence of amp alone (no tet) and then seeded onto NGM plates containing amp and 1 mM IPTG. This technique does not work well if the cells are old; therefore, the strain should be seeded onto IPTG-containing plates from a fresh overnight that was grown from a colony on an amp/tet plate. Biosafety Level: BSL-1. For more info see http://www.wormbook.org/wli/wbg17.1p32/ Mutagen: Outcrossed: x Made by: Killian/Hubbard Received: 03/05/01 NYU Grossman School of Medicine ----------------------------------------------------------------------------- Strain: GC463 Species: Caenorhabditis elegans Genotype: rpl-11.1(ar228) V/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Sterile non-Unc (homozygous for ar228) and dead eggs. ar228 have severely reduced germline proliferation, which is more severe at 15C than at 25C. Mutagen: EMS Outcrossed: x>4 Made by: Jane Hubbard Received: 03/24/06 NYU Grossman School of Medicine ----------------------------------------------------------------------------- Strain: GC565 Species: Caenorhabditis elegans Genotype: pro-1(na48)/mIn1 [dpy-10(e128) mIs14] II. Description: Heterozygotes are WT with GFP+ (pharynx) and segregate Dpy with GFP+ (pharynx) and slow growing GFP- animals which are generally sterile (with germline tumor at 25C). pro-1(na48) is a weak, recessive, loss-of-function allele that behaves as a stronger loss-of-function at lower temperatures. At 25C, 85% of na48 animals will develop a proximal germline tumorr. Although tumors are less common at lower temperatures, the animals are generally sterile due to low proliferation and somatic gonad defects. na48 animals are slow growing at all temperatures. Mutagen: EMS Outcrossed: x6. Made by: Jane Hubbard Received: 08/11/04 NYU Grossman School of Medicine ----------------------------------------------------------------------------- Strain: GC589 Species: Caenorhabditis elegans Genotype: pab-1(ar232)/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT and GFP+, and segregate Sterile non-GFP (homozygous ar232) and dead eggs. ar232 have severely reduced germline proliferation. Mutagen: EMS Outcrossed: x1 Made by: John Maciejowski Received: 03/24/06 NYU Grossman School of Medicine ----------------------------------------------------------------------------- Strain: GC613 Species: Caenorhabditis elegans Genotype: eef-1A.1(ar229) III/eT1 (III;V). Description: Heterozygotes are WT and segregate WT, Steriles (homozygous ar229), Unc-36 (homozygous eT1), and dead eggs. ar229 have severely reduced germline proliferation. Mutagen: EMS Outcrossed: x1 Made by: Patricia G Cipriani Received: 03/24/06 NYU Grossman School of Medicine ----------------------------------------------------------------------------- Strain: GC734 Species: Caenorhabditis elegans Genotype: pro-2(na27) II. Description: At 20C and below, worms have smaller brood size than WT, although the gonad develops properly. At 25C, worms are sterile (either Glp or Pro). The animals grow slower than WT at all temperatures. Maintain at 20C or below. Mutagen: EMS Outcrossed: x2 Made by: E. Jane Hubbard Received: 04/23/07 NYU Grossman School of Medicine ----------------------------------------------------------------------------- Strain: GC735 Species: Caenorhabditis elegans Genotype: pro-3(ar226) V. Description: At 20C and below, worms are visibly WT; animals grow somewhat larger than WT. At 25C, segregates WT, Glp and Pro. Maintain at 20C or below. Mutagen: EMS Outcrossed: x4 Made by: E. Jane Hubbard Received: 04/23/07 NYU Grossman School of Medicine ----------------------------------------------------------------------------- Strain: GC771 Species: Caenorhabditis elegans Genotype: him-5(e1490) V; naEx57. Description: naEx57 [lag-2p::FLP::let-858 3'UTR + ceh-22p::GFP]. Him. Derived by injection of plasmids pGC158 and pCW2.19. Reference: Voutov, R and Hubbard, EJ. Genetics 180(1):103-19 (2008). Mutagen: Outcrossed: x0 Made by: R. Voutev/J. Hubbard Received: 03/15/25 NYU Grossman School of Medicine ----------------------------------------------------------------------------- Strain: GC773 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; naIs3. Description: naIs3 [(pGC133) hsp-16.41::FLP:::let-858 3'UTR) + Cbr-unc-119(+)]. Mutagen: Outcrossed: x0 Made by: R. Voutev/J. Hubbard Received: 10/25/12 NYU Grossman School of Medicine ----------------------------------------------------------------------------- Strain: GC817 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; naIs6. Description: naIs6 [hsp-16.2p::FLP::let-858 3'UTR + Cbr-unc-119(+) + ceh-22p::GFP)]. Mutagen: Outcrossed: x1 Made by: R. Voutev/J. Hubbard Received: 07/15/09 NYU Grossman School of Medicine ----------------------------------------------------------------------------- Strain: GC822 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; naEx75. Description: naEx75 [(pGC146) hsp-16.2p::FLP::let-858 3'UTR) + Cbr-unc-119(+) + (pCW2.1) ceh-22p::GFP)]. Array was bombarded but did not integrate. Mutagen: Outcrossed: x0 Made by: R. Voutev/J. Hubbard Received: 07/15/09 NYU Grossman School of Medicine ----------------------------------------------------------------------------- Strain: GC827 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; naIs7. Description: naIs7 [hsp-16.2p::FLP::let-858 3'UTR + Cbr-unc-119(+)]. Does not express ceh-22p::GFP, but unc-119 is rescued. Mutagen: Outcrossed: x0 Made by: R. Voutev/J. Hubbard Received: 07/15/09 NYU Grossman School of Medicine ----------------------------------------------------------------------------- Strain: GC833 Species: Caenorhabditis elegans Genotype: glp-1(ar202) III. Description: Temperature sensitive. Maintain at 15C. Mutagen: EMS Outcrossed: x Made by: Jane Hubbard Received: 02/19/08 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: GD211 Species: Caenorhabditis elegans Genotype: rol-6(e187) hlh-6(tm299) unc-4(e120) II. Description: Mutagen: Outcrossed: x>5 Made by: Ryan Smit Received: 01/19/09 University of Calgary, Calgary, Alberta, Canada ----------------------------------------------------------------------------- Strain: GE1076 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; lon-2(e678) X. Description: Long. Maintain at 15C. pha-1(e2123) is a temperature sensitive embryonic lethal. Strain viable only at 15C. lon-2 included to facilitate microinjection. Check regularly for ts lethality-spontaneous sup-35 mutations may occur! Mutagen: Outcrossed: x Made by: Heinke Schnabel Received: 08/02/94 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE1210 Species: Caenorhabditis elegans Genotype: dpy-2(e8) ooc-3(t1308)/mnC1 [dpy-10(e128) unc-52(e444)] II; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, Dpys which produce dead eggs, and DpyUncs. Mutagen: EMS Outcrossed: x2 Made by: H. & R. Schnabel Received: 09/21/00 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE1386 Species: Caenorhabditis elegans Genotype: tDf3 dpy-5(e61)/szT1 [lon-2(e678)] I; dpy-8(sc44)/szT1 X. Description: Heterozygotes are WT and segregate WT, Lon males and dead eggs. Strain will occasionally throw some DpysRollers. Mutagen: EMS Outcrossed: x2 Made by: Richard Feichtinger Received: 02/25/93 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE1549 Species: Caenorhabditis elegans Genotype: tDf4 dpy-5(e61)/szT1 [lon-2(e678)] I; dpy-8(sc44)/szT1 X. Description: Heterozygotes are WT and segregate WT, dead eggs, and Lon males. Maintain by picking WT. Strain will occasionally throw some DpysRollers. Mutagen: EMS Outcrossed: x>4 Made by: Richard Feichtinger Received: 09/23/92 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE1708 Species: Caenorhabditis elegans Genotype: dpy-2(e8) unc-4(e120) II. Description: DpyUnc. Mutagen: Outcrossed: x Made by: Bayle F Received: 08/16/91 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE1709 Species: Caenorhabditis elegans Genotype: vab-9(e1744) unc-4(e120) II; him-5(e1490) V. Description: Unc. Slightly Dpy. Tail whip knobbed at all stages except adult male (adult male tail tip slightly swollen). Variably Egl. Mutagen: Outcrossed: x Made by: Bayle F Received: 08/16/91 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE1710 Species: Caenorhabditis elegans Genotype: rol-6(e187) unc-4(e120) II. Description: Roller Unc. Mutagen: Outcrossed: x Made by: Bayle F Received: 08/16/91 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE1711 Species: Caenorhabditis elegans Genotype: dpy-2(e8) vab-9(e1744) unc-4(e120) II. Description: Dpy. Unc. Tail whip knobbed at all stages except adult male (adult male tail tip slightly swollen). Mutagen: Outcrossed: x Made by: Bayle F Received: 08/16/91 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE1712 Species: Caenorhabditis elegans Genotype: vab-9(e1744) rol-6(e187) unc-4(e120) II. Description: Unc. Roller. Tail whip knobbed at all stages except adult male (adult male tail tip slightly swollen). Mutagen: Outcrossed: x Made by: Bayle F Received: 08/16/91 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE1713 Species: C. elegans Genotype: unc-32(e189) ZK688.9(t1433)/qC1[dpy-19(1259) glp-1(q339)] III; him-3(e1147) IV. Description: Maternal-effect lethal mutation linked to unc-32(e189) and balanced by glp-1- and dpy-19-marked recombination suppressor. Heterozygotes are WT, and segregate WT, sterile ts-Dpy qC1 homozygotes, and viable Unc t1433 homozygotes that produce arrested embryos (approximately 100 cells). GE1713 is also homozygous for him-3(e1147) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE1735 Species: C. elegans Genotype: unc-32(e189) top-3(t1470)/qC1[dpy-19(1259) glp-1(q339)] III; him-3(e1147) IV. Description: Maternal-effect lethal mutation linked to unc-32(e189) and balanced by glp-1- and dpy-19-marked recombination suppressor. Heterozygotes are WT, and segregate WT, sterile ts-Dpy qC1 homozygotes, and viable Unc t1470 homozygotes that produce arrested embryos. GE1735 is also homozygous for him-3(e1147) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE1742 Species: C. elegans Genotype: unc-32(e189) bckd-1A(t1461)/qC1[dpy-19(1259) glp-1(q339)] III; him-3(e1147) IV. Description: Maternal-effect lethal mutation linked to unc-32(e189) and balanced by glp-1- and dpy-19-marked recombination suppressor. Heterozygotes are WT, and segregate WT, sterile ts-Dpy qC1 homozygotes, and viable Unc t1461 homozygotes that produce dead eggs. GE1742 is also homozygous for him-3(e1147) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE1825 Species: Caenorhabditis elegans Genotype: tDf1/unc-32(e189) dpy-18(e499) III. Description: Heterozygotes are Dpy and segregate Dpy, DpyUnc and dead eggs. Mutagen: X Rays Outcrossed: x Made by: H. & R. Schnabel Received: 07/18/94 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE1936 Species: C. elegans Genotype: xpf-1(e1487) II; unc-24(e138) atg-7(t1738)/nT1[let (m435)] IV; dpy-11(e224)/nT1[let (m435)] V. Description: Maternal effect lethal mutation linked to unc-24(e138) and pseudolinked to dpy-11(e224), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t1738 homozygotes that produce dead embryos. GE1936 is also homozygous for xpf-1(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. xpf-1 previously known as him-9. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE1939 Species: C. elegans Genotype: xpf-1(e1487) II; unc-24(e138) trcs-1(t1745)/nT1[let (m435)] IV; dpy-11(e224)/nT1[let (m435)] V. Description: Temperature-sensitive maternal effect lethal mutation linked to unc-24(e138) and pseudolinked to dpy-11(e224), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t1745 homozygotes that produce dead eggs at restrictive temperature. GE1939 is also homozygous for xpf-1(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. xpf-1 previously known as him-9. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE1958 Species: C. elegans Genotype: xpf-1(e1487) II; unc-24(e138) atg-7(t1726)/nT1[let (m435)] IV; dpy-11(e224)/nT1[let (m435)] V. Description: Maternal effect lethal mutation linked to unc-24(e138) and pseudolinked to dpy-11(e224), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t1726 homozygotes that produce dead embryos. GE1958 is also homozygous for xpf-1(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. xpf-1 previously known as him-9. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE2001 Species: Caenorhabditis elegans Genotype: xpf-1(e1487) II; unc-24(e138) IV/nT1(IV;V); dpy-11(e224) ccz-1(t2070) V/nT1 (IV;V). Description: Him. Wild-type heterozygotes segregate WT heterozygotes and Dpy Unc ccz-1 homozygotes (produce only arrested embryos). ccz-1 embryos have spindle orientation defects, accumulate vesicles, and problems engulfing apoptotic corpses. Reference: Nieto C, et al. J Cell Sci. 2010 Jun 15;123(Pt 12):2001-7. Mutagen: EMS Outcrossed: x>3 Made by: R. Schnable Received: 10/20/10 Center for Biomedical Research of La Rioja, Logrono, Spain ----------------------------------------------------------------------------- Strain: GE2047 Species: C. elegans Genotype: xpf-1(e1487) II; unc-24(e138)/nt1[let (m435)] IV; dpy-11(e224) cept-2(t2021)/nt1[let (m435)] V. Description: Temperature-sensitive maternal effect lethal mutation linked to dpy-11(e224) and pseudolinked to unc-24(e138), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t2021 homozygotes that produce dead eggs at restrictive temperature. GE2047 is also homozygous for xpf-1(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. xpf-1 previously known as him-9. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE2122 Species: C. elegans Genotype: xpf-1(e1487) II; unc-24(e138)/nt1[let (m435)] IV; dpy-11(e224) cept-2(t2007)/nt1[let (m435)] V. Description: Maternal effect lethal mutation linked to dpy-11(e224) and pseudolinked to unc-24(e138), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t2007 homozygotes that produce dead eggs. GE2122 is also homozygous for xpf-1(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. xpf-1 previously known as him-9. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE2153 Species: C. elegans Genotype: xpf-1(e1487) II; unc-24(e138) wapl-1(t1773)/nt1[let (m435)] IV; dpy-11(e224)/nt1[let (m435)] V. Description: Maternal effect lethal mutation linked to unc-24(e138) and pseudolinked to dpy-11(e224), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t1773 homozygotes that do not produce viable progeny. GE2153 is also homozygous for xpf-1(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. xpf-1 previously known as him-9. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE2158 Species: Caenorhabditis elegans Genotype: tDf2/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Homozygous tDf2 is embryonic lethal. Maintain by picking WT. Mutagen: X-rays Outcrossed: x Made by: H. & R. Schnabel Received: 02/23/94 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2167 Species: Caenorhabditis elegans Genotype: unc-32(e189) tDf5/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, Sterile Dpys and dead eggs. Throws males. Maintain by picking WT. See WBG 13 #4 p.96. Mutagen: X-rays Outcrossed: x2 Made by: H. & R. Schnabel Received: 10/11/94 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2171 Species: Caenorhabditis elegans Genotype: tDf9 unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles and dead eggs, and males. tDf9 is embryonic lethal. Mutagen: X-ray Outcrossed: x2 Made by: H & R Schnabel Received: 04/12/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2172 Species: Caenorhabditis elegans Genotype: unc-32(e189) tDf8/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Maintain by picking WT. See WBG 13 (4): 96. Mutagen: X-ray Outcrossed: x2 Made by: H. & R. Schnabel Received: 11/07/94 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2175 Species: Caenorhabditis elegans Genotype: unc-32(e189) tDf6/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, Sterile Dpys and dead eggs. Throws males. Maintain by picking WT. See WBG 13 #4 p.96. Mutagen: X-rays Outcrossed: x2 Made by: H. & R. Schnabel Received: 10/11/94 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2180 Species: Caenorhabditis elegans Genotype: unc-32(e189) tDf7/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, Sterile Dpys and dead eggs. Throws males. Maintain by picking WT. See WBG 13 #4 p.96. Mutagen: X-rays Outcrossed: x2 Made by: H. & R. Schnabel Received: 10/11/94 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2198 Species: Caenorhabditis elegans Genotype: cta-3(t1492) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2199 Species: Caenorhabditis elegans Genotype: unc-32(e189) nup-1(t1482)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2200 Species: C. elegans Genotype: unc-32(e189) bckd-1A(t1480)/qC1[dpy-19(1259) glp-1(q339)] III; him-3(e1147) IV. Description: Maternal-effect lethal mutation linked to unc-32(e189) and balanced by glp-1- and dpy-19-marked recombination suppressor. Heterozygotes are WT, and segregate WT, sterile ts-Dpy qC1 homozygotes, and viable Unc t1480 homozygotes that produce dead eggs. GE2200 is also homozygous for him-3(e1147) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE2204 Species: Caenorhabditis elegans Genotype: unc-32(e189) tDf10/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles and dead eggs, and males. tDf10 is embryonic lethal. Mutagen: X-rays Outcrossed: x2 Made by: H & R Schnabel Received: 02/05/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2211 Species: Caenorhabditis elegans Genotype: unc-32(e189) sas-1(t1476)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2212 Species: Caenorhabditis elegans Genotype: unc-32(e189) com-1(t1489)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, Dpy Steriles, and Uncs which are Mel with over 99% embryonic lethality. Mutagen: EMS Outcrossed: x2 Made by: Schnabel Lab Received: 11/30/07 University of Vienna, Vienna, Austria ----------------------------------------------------------------------------- Strain: GE2219 Species: Caenorhabditis elegans Genotype: unc-32(e189) csg-1(t1501)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2237 Species: C. elegans Genotype: unc-32(e189) pod-1(t1614)/qC1[dpy-19(1259) glp-1(q339)] III; him-3(e1147) IV. Description: Maternal-effect lethal mutation linked to unc-32(e189) and balanced by glp-1- and dpy-19-marked recombination suppressor. Heterozygotes are WT, and segregate WT, sterile ts-Dpy qC1 homozygotes, and viable Unc t1614 homozygotes that do not produce viable progeny. GE2237 is also homozygous for him-3(e1147) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE2238 Species: Caenorhabditis elegans Genotype: cyk-1(t1611) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, Sterile Dpys and Uncs which give only dead eggs. Throws males. Mutagen: Schnabels Outcrossed: x Made by: EMS Received: 04/05/99 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: GE2239 Species: Caenorhabditis elegans Genotype: unc-32(e189) csg-7(t1612)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2240 Species: Caenorhabditis elegans Genotype: apo-3(t1613) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2242 Species: Caenorhabditis elegans Genotype: cta-1(t1618) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2244 Species: Caenorhabditis elegans Genotype: unc-32(e189) lit-1(t1512)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles and Uncs which produce only dead eggs at 25C but viable progeny at 15C. Throws males. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 04/30/98 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2245 Species: Caenorhabditis elegans Genotype: unc-32(e189) tim-1(t1545)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. tim-1 is temperature sensitive: progeny are viable at 15C, and dead at 25C. Previously called csg-5(t1545). Received new stock 5/04. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2255 Species: Caenorhabditis elegans Genotype: unc-32(e189) tbb-2(t1623)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2258 Species: Caenorhabditis elegans Genotype: unc-32(e189) com-1(t1626)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, Dpy Steriles, and Uncs which are Mel with over 99% embryonic lethality. Mutagen: EMS Outcrossed: x2 Made by: Schnabel Lab Received: 11/30/07 University of Vienna, Vienna, Austria ----------------------------------------------------------------------------- Strain: GE2259 Species: Caenorhabditis elegans Genotype: unc-32(e189) cta-5(t1627)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2277 Species: C. elegans Genotype: unc-32(e189) such-1(t1496)/qC1[dpy-19(1259) glp-1(q339)] III; him-3(e1147) IV. Description: Maternal-effect lethal mutation linked to unc-32(e189) and balanced by glp-1- and dpy-19-marked recombination suppressor. Heterozygotes are WT, and segregate WT, sterile ts-Dpy qC1 homozygotes, and viable Unc t1496 homozygotes that do not produce viable progeny. GE2277 is also homozygous for him-3(e1147) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE2305 Species: C. elegans Genotype: xpf-1(e1487) II; unc-24(e138) wapl-1(t1867)/nt1[let (m435)] IV; dpy-11(e224)/nT1[let (m435)] V. Description: Maternal effect lethal mutation linked to unc-24(e138) and pseudolinked to dpy-11(e224), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t1867 homozygotes that do not produce viable progeny. GE2305 is also homozygous for xpf-1(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. xpf-1 previously known as him-9. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE2335 Species: C. elegans Genotype: xpf-1(e1487) II; unc-24(e138)/nT1[let (m435)] IV; dpy-11(e224) dlat-1(t2056)/nT1[let (m435)] V. Description: Maternal effect lethal mutation linked to dpy-11(e224) and pseudolinked to unc-24(e138), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t2056 homozygotes that produce dead eggs. GE2335 is also homozygous for xpf-1(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. xpf-1 previously known as him-9. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE2345 Species: Caenorhabditis elegans Genotype: cyk-3(t1525) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2349 Species: Caenorhabditis elegans Genotype: unc-32(e189) pnm-5(t1543)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2353 Species: Caenorhabditis elegans Genotype: unc-32(e189) lit-1(t1534)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, Uncs which produce dead eggs, and Dpy Steriles. Throws males. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 09/05/00 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2358 Species: Caenorhabditis elegans Genotype: unc-32(e189) pnm-4(t1491)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2359 Species: Caenorhabditis elegans Genotype: unc-32(e189) amo-5(t1478)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, Dpy Steriles, and embryonic lethals. Throws males. t1478 PKA arm-5. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 07/25/06 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2364 Species: Caenorhabditis elegans Genotype: let-771(t1554) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2391 Species: C. elegans Genotype: xpf-1(e1487) II; unc-24(e138) perm-5(t1932)/nT1[let (m435)] IV; dpy-11(e224)/nT1[let (m435)] V. Description: Maternal effect lethal mutation linked to unc-24(e138) and pseudolinked to dpy-11(e224), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t1932 homozygotes that produce dead embryos. GE2391 is also homozygous for xpf-1(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. xpf-1 previously known as him-9. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE2399 Species: C. elegans Genotype: unc-32(e189) top-3(t1559)/qC1[dpy-19(1259) glp-1(q339)] III; him-3(e1147) IV. Description: Maternal-effect lethal mutation linked to unc-32(e189) and balanced by glp-1- and dpy-19-marked recombination suppressor. Heterozygotes are WT, and segregate WT, sterile ts-Dpy qC1 homozygotes, and viable Unc t1559 homozygotes that produce arrested embryos. GE2399 is also homozygous for him-3(e1147) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE24 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III. Description: Wild type at 15C. Embryonic lethal at 25C. Temperature sensitive phase during embryogenesis. sup-35 mutations may (and have) occurred spontaneously in this strain. This can be checked by shifting the worms to 25C: pha-1 animals should be dead while sup-35 pha-1 animals will live. Mutagen: EMS Outcrossed: x3 Made by: H. & R. Schnabel Received: 07/08/94 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2453 Species: C. elegans Genotype: xpf-1(e1487) II; unc-24(e138) perm-5(t1900)/nT1[let (m435)] IV; dpy-11(e224)/nT1[let (m435)] V. Description: Maternal effect lethal mutation linked to unc-24(e138) and pseudolinked to dpy-11(e224), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t1900 homozygotes that produce dead embryos. GE2453 is also homozygous for xpf-1(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. xpf-1 previously known as him-9. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE2512 Species: C. elegans Genotype: xpf-1(e1487) II; unc-24(e138) trcs-1(t1909)/nT1[let (m435)] IV; dpy-11(e224)/nT1[let (m435)] V. Description: Leaky temperature-sensitive maternal effect lethal mutation linked to unc-24(e138) and pseudolinked to dpy-11(e224), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t1909 homozygotes that produce dead eggs at restrictive temperature. GE2512 is also homozygous for xpf-1(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. xpf-1 previously known as him-9. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE2516 Species: Caenorhabditis elegans Genotype: xpf-1(e1487) II; unc-24(e138) IV/nT1(IV;V); dpy-11(e224) ccz-1(t2071) V/nT1 (IV;V). Description: Him. Wild-type heterozygotes segregate WT heterozygotes and Dpy Unc ccz-1 homozygotes (produce only arrested embryos). ccz-1 embryos have spindle orientation defects, accumulate vesicles, and problems engulfing apoptotic corpses. Reference: Nieto C, et al. J Cell Sci. 2010 Jun 15;123(Pt 12):2001-7. Mutagen: EMS Outcrossed: x>3 Made by: R. Schnable Received: 10/20/10 Center for Biomedical Research of La Rioja, Logrono, Spain ----------------------------------------------------------------------------- Strain: GE2533 Species: Caenorhabditis elegans Genotype: unc-32(e189) zyg-8(t1638)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. zyg-8(t1638) pka apo-1(t1638). SeeWBPaper00005199. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2534 Species: Caenorhabditis elegans Genotype: csg-2(t1637) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2541 Species: C. elegans Genotype: xpf-1(e1487) II; unc-24(e138)/nT1[let (m435)] IV; dpy-11(e224) dlat-1(t2035)/nT1[let (m435)] V. Description: Maternal effect lethal mutation linked to dpy-11(e224) and pseudolinked to unc-24(e138), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t2035 homozygotes that produce dead eggs. GE2541 is also homozygous for xpf-1(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. xpf-1 previously known as him-9. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE2576 Species: Caenorhabditis elegans Genotype: unc-32(e189) baf-1(t1639)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2605 Species: C. elegans Genotype: unc-32(e189) pod-1(t1674)/qC1[dpy-19(1259) glp-1(q339)] III; him-3(e1147) IV. Description: Maternal-effect lethal mutation linked to unc-32(e189) and balanced by glp-1- and dpy-19-marked recombination suppressor. Heterozygotes are WT, and segregate WT, sterile ts-Dpy qC1 homozygotes, and viable Unc t1674 homozygotes that do not produce viable progeny. GE2605 is also homozygous for him-3(e1147) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE2606 Species: Caenorhabditis elegans Genotype: rot-3(t1640) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2614 Species: Caenorhabditis elegans Genotype: unc-32(e189) faf-1(t1678)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2621 Species: C. elegans Genotype: unc-32(e189) ZK688.9(t1587)/qC1[dpy-19(1259) glp-1(q339)] III; him-3(e1147) IV. Description: Maternal-effect lethal mutation linked to unc-32(e189) and balanced by glp-1- and dpy-19-marked recombination suppressor. Heterozygotes are WT, and segregate WT, sterile ts-Dpy qC1 homozygotes, and viable Unc t1587 homozygotes that produce arrested embryos. GE2621 is also homozygous for him-3(e1147) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE2626 Species: Caenorhabditis elegans Genotype: ran-2(t1598) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2628 Species: Caenorhabditis elegans Genotype: unc-32(e189) rot-1(t1599)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2629 Species: Caenorhabditis elegans Genotype: sas-2(t1595) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2633 Species: Caenorhabditis elegans Genotype: mel-28(t1684) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2636 Species: Caenorhabditis elegans Genotype: unc-32(e189) cyk-4(t1689)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Received new stock 3/01. See also WBPaper00005208. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2638 Species: Caenorhabditis elegans Genotype: unc-32(e189) csg-3(t1685)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2656 Species: Caenorhabditis elegans Genotype: cta-2(t1701) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2659 Species: Caenorhabditis elegans Genotype: unc-32(e189) apo-4(t1707)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2663 Species: Caenorhabditis elegans Genotype: unc-32(e189) pnm-6(t1586)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2666 Species: C. elegans Genotype: unc-32(e189) such-1(t1693)/qC1[dpy-19(1259) glp-1(q339)] III; him-3(e1147) IV. Description: Maternal-effect lethal mutation linked to unc-32(e189) and balanced by glp-1- and dpy-19-marked recombination suppressor. Heterozygotes are WT, and segregate WT, sterile ts-Dpy qC1 homozygotes, and viable Unc t1693 homozygotes that do not produce viable progeny. GE2666 is also homozygous for him-3(e1147) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE2685 Species: Caenorhabditis elegans Genotype: unc-32(e189) csg-8(t1714)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2690 Species: Caenorhabditis elegans Genotype: let-733(t1683) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2720 Species: Caenorhabditis elegans Genotype: cta-4(t1562) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2722 Species: Caenorhabditis elegans Genotype: cyk-1(t1568) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, Sterile Dpys and Uncs which give only dead eggs. Throws males. Mutagen: Schnabels Outcrossed: x Made by: EMS Received: 04/05/99 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: GE2723 Species: Caenorhabditis elegans Genotype: unc-32(e189) csg-6(t1556)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2727 Species: Caenorhabditis elegans Genotype: nup-2(t1574) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2730 Species: Caenorhabditis elegans Genotype: unc-32(e189) lis-1(t1550)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. t1550 pka pnm-1(t1550). Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2734 Species: C. elegans Genotype: xpf-1(e1487) II; unc-24(e138)/nt1[let (m435)] IV; dpy-11(e224) C56A3.8(t2029)/nt1[let (m435)] V. Description: Maternal effect lethal mutation linked to dpy-11(e224) and pseudolinked to unc-24(e138), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t2029 homozygotes that produce unfertilized oocytes. GE2734 is also homozygous for xpf-1(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. xpf-1 previously known as him-9. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE2827 Species: C. elegans Genotype: xpf-1(e1487) II; unc-24(e138) T22B11.1(t1786)/nt1[let (m435)] IV; dpy-11(e224)/nt1[let (m435)] V. Description: Temperature-sensitive maternal effect lethal mutation linked to unc-24(e138) and pseudolinked to dpy-11(e224), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t1786 homozygotes that produce unfertilized oocytes at restrictive temperature. GE2827 is also homozygous for xpf-1(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. xpf-1 previously known as him-9. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE2886 Species: C. elegans Genotype: xpf-1(e1487) II; unc-24(e138)/nt1[let (m435)] IV; dpy-11(e224) C56A3.8(t2055)/nt1[let (m435)] V. Description: Maternal effect lethal mutation linked to dpy-11(e224) and pseudolinked to unc-24(e138), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t2055 homozygotes that produce unfertilized oocytes. GE2886 is also homozygous for xpf-1(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. xpf-1 previously known as him-9. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE2895 Species: C. elegans Genotype: xpf-1(e1487) II; unc-24(e138) T22B11.1(t1866)/nt1[let (m435)] IV; dpy-11(e224)/nt1[let (m435)] V. Description: Temperature-sensitive maternal effect lethal mutation linked to unc-24(e138) and pseudolinked to dpy-11(e224), and balanced by recessive lethal translocation. Heterozygotes are WT and segregate WT, arrested nT1 aneuploids, arrested nT1 homozygotes, and viable DpyUnc t1866 homozygotes that produce unfertilized oocytes at restrictive temperature. GE2895 is also homozygous for xpf-1(e1487) so that male progeny are segregated. Pick WT and check for correct segregation of progeny to maintain. Please reference Li-Leger et al., G3 11(12) 2021 in any work resulting from use of this mutation. xpf-1 previously known as him-9. Mutagen: EMS Outcrossed: x1 Made by: Vancouver KO Group Received: 03/08/22 University of British Columbia, Vancouver BC, Canada ----------------------------------------------------------------------------- Strain: GE2929 Species: Caenorhabditis elegans Genotype: unc-32(e189) pna-2(t1434)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2935 Species: Caenorhabditis elegans Genotype: let-725(t1440) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. t1440 previously called mel-27. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2940 Species: Caenorhabditis elegans Genotype: unc-32(e189) pnm-2(t1445)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2941 Species: Caenorhabditis elegans Genotype: unc-32(e189) let-(t1446)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles and Uncs which give only dead eggs. This strain was mistakenly called emb-30 in the paper; it is not an emb-30 allele. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2946 Species: Caenorhabditis elegans Genotype: let-748(t1452) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2948 Species: Caenorhabditis elegans Genotype: unc-32(e189) apo-2(t1454)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2952 Species: Caenorhabditis elegans Genotype: unc-32(e189) pnm-3(t1458)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2959 Species: Caenorhabditis elegans Genotype: unc-32(e189) tbg-1(t1465)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. tbg-1(t1465) previously called sas-3(t1465). Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE2961 Species: Caenorhabditis elegans Genotype: unc-32(e189) csg-4(t1467)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE3023 Species: Caenorhabditis elegans Genotype: emb-8(t1533) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: EMS Outcrossed: x2 Made by: H & R Schnabel Received: 10/06/99 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE31 Species: Caenorhabditis elegans Genotype: cib-1(e2300) I. Description: Wild type at 15C. Maternal effect embryonic lethal at 25C. Temperature sensitive period in oocyte and early embryogenesis. Mutagen: EMS Outcrossed: x2 Made by: R. & H. Schnabel Received: 07/08/94 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE337 Species: Caenorhabditis elegans Genotype: pha-1(e2123) dpy-18(e499) III; sup-37(e2215) V. Description: sup-37 suppresses temperature sensitive embryonic lethality of e2123. Strain is viable at 15C and 25C. Dpy. Behavioural mutation leading to avoidance of bacteria (worms tend to concentrate just outside the lawn) unlinked to sup-37. Mutagen: EMS Outcrossed: x Made by: H & R Schnabel Received: 08/02/94 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE341 Species: Caenorhabditis elegans Genotype: pha-1(e2123) dpy-18(e499) III; sup-36(e2217) IV. Description: sup-36 suppresses temperature sensitive lethal phenotype of e2123. Strain is viable at 15C and 25C. Dpy. Mutagen: EMS Outcrossed: x Made by: H & R Schnabel Received: 08/02/94 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE346 Species: Caenorhabditis elegans Genotype: sup-35(e2223) pha-1(e2123) dpy-18(e499) III. Description: sup-35 suppresses the temperature sensitive embryonic lethality of e2123. Strain is viable at 15C and 25C. Dpy. Mutagen: EMS Outcrossed: x Made by: H. & R. Schnabel Received: 08/02/94 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE3633 Species: Caenorhabditis elegans Genotype: unc-32(e189) cyk-4(t1689)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-3(e1147) IV. Description: Heterozygotes are WT and segregate WT, DpySteriles, and Uncs which give only dead eggs. Mutagen: Outcrossed: x Made by: Received: 07/13/07 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE3841 Species: Caenorhabditis elegans Genotype: unc-32(e189) tim-1(t1545) III. Description: Unc. Temperature sensitive tim-1. Viable at 15C. Mel at 25C. Mutagen: EMS Outcrossed: x2 Made by: Received: 05/10/04 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE4132 Species: Caenorhabditis elegans Genotype: unc-32(e189) com-1(t1626)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, Dpy Steriles, and Uncs which are Mel with over 99% embryonic lethality. Mutagen: EMS Outcrossed: x3 Made by: Schnabel Lab Received: 11/30/07 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE42 Species: Caenorhabditis elegans Genotype: vab-7(e1562) pha-1(e2123) III. Description: Temperature sensitive. Maintain at 15C. pha-1(e2123) is embryonic lethal at 25C. Hermaphrodites have an abnormal tail: sometimes twisted, tail whip never formed, often bobbed; sometimes uncoordinated with bent tail. Adult male tail deformed also. Mutagen: Outcrossed: x Made by: Heinke Schnabel Received: 08/02/94 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE4345 Species: Caenorhabditis elegans Genotype: smc-3(t2553) III. Description: Temperature-sensitive, maintain at 15C. Shifitng L1 larvae to 25C causes fully penetrant meiosis defect (~3% viability). L4 larvae shifted to 25C produce ~15 progeny. Reference: Baudrimont A, PLoS One. 2011;6(9):e24799. Mutagen: Outcrossed: x Made by: Schnabel & Jantsch Received: 10/27/11 University of Vienna, Vienna, Austria ----------------------------------------------------------------------------- Strain: GE68 Species: Caenorhabditis elegans Genotype: glp-1(e2144) III. Description: Sterile at 25C, maintain at 15C. NOTE (11/16/10 - J. Hubbard): This strain is NOT synonymous with glp-1(e2141) as previously reported in Kodoyianni V, Maine EM, Kimble J. (1992) [Molecular basis of loss-of-function mutations in the glp-1 gene of Caenorhabditis elegans. Mol Biol Cell. 3,1199-213. PMID: 1457827]. As reported in WBG article by Dalfó D, Priess J, Schnabel R and Hubbard J. (2010) [glp-1(e2141) sequence correction. The Worm Breeders Gazette. 18-3.], e2144 carries the mutation c2785t in exon 8, leading to the amino acid change L929F, whereas e2141 carries the mutations c2920t and a3610g in exon 8, leading to the amino acid changes R974C and T1204A. Mutagen: EMS Outcrossed: x Made by: R & H Schnabel Received: 11/16/10 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GE974 Species: Caenorhabditis elegans Genotype: cib-1(e2300) dpy-5(e61) I. Description: Dpy. cib-1 is a temperature sensitive maternal effect lethal. Grow at 15C. Produces dead eggs at 25C. Mutagen: Outcrossed: x Made by: Heinke Schnabel Received: 08/02/94 Technische Universitat Braunschweig, Braunschweig, Germany ----------------------------------------------------------------------------- Strain: GF63 Species: Caenorhabditis elegans Genotype: dgEx63. Description: dgEx63[pAMS52 vha-6p::Q0::YFP + rol-6(su1006) + pBluescript II]. Maintain by picking Rollers. Diffuse distribution of Q0::YFP throughout the intestine. Mutagen: Outcrossed: x0 Made by: A. Mohri-Shiomi Received: 01/11/08 University of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: GF66 Species: Caenorhabditis elegans Genotype: dgEx66. Description: dgEx66 [(pAMS58) vha-6p::Q82::YFP + rol-6(su1006) + pBluescript II]. Maintain by picking Rollers. Q82::YFP is completely aggregated throughout the intestine. Mutagen: Outcrossed: x0 Made by: A. Mohri-Shiomi Received: 01/11/08 University of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: GF72 Species: Caenorhabditis elegans Genotype: dgEx72. Description: dgEx72[pAMS54 vha-6p::Q33::YFP + rol-6(su1006) + pBluescript II]. Maintain by picking Rollers. Diffuse distribution of Q33::YFP throughout the intestine. Received new stock 6/2008. Mutagen: Outcrossed: x0 Made by: A. Mohri-Shiomi Received: 01/11/08 University of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: GF78 Species: Caenorhabditis elegans Genotype: dgEx78. Description: dgEx78 [(pAMS68) vha-6p::Q40::YFP + rol-6(su1006) + pBluescript II]. Maintain by picking Rollers. Most worms with dgEx78 have diffuse fluorescence of Q40::YFP throughout the intestine, but some have a mixture of diffuse and focal fluoresence. Mutagen: Outcrossed: x0 Made by: A. Mohri-Shiomi Received: 01/11/08 University of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: GF80 Species: Caenorhabditis elegans Genotype: dgEx80. Description: dgEx80 [(pAMS66) vha-6p::Q44::YFP + rol-6(su1006) + pBluescript II]. Maintain by picking Rollers. Most worms with dgEx80 have diffuse fluorescence of Q44::YFP throughout the intestine, but some have a mixture of diffuse and focal fluoresence. Mutagen: Outcrossed: x0 Made by: A. Mohri-Shiomi Received: 01/11/08 University of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: GF83 Species: Caenorhabditis elegans Genotype: dgEx83. Description: dgEx83[pAMS56 vha-6p::Q64::YFP + rol-6(su1006) + pBluescript II]. Maintain by picking Rollers. Q64::YFP is completely aggregated throughout the intestine. Mutagen: Outcrossed: x0 Made by: A. Mohri-Shiomi Received: 01/11/08 University of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: GG1 Species: Caenorhabditis elegans Genotype: emb-11(g1) IV. Description: Temperature sensitive, maintain at 15C. At 25C, arrests at 1 to 24 cell stage. Will grow at 20C. Mutagen: EMS Outcrossed: x Made by: Received: 05/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG14 Species: Caenorhabditis elegans Genotype: fasn-1(g14) I. Description: Temperature sensitive. Grow at 15C. Mutagen: EMS Outcrossed: x Made by: Received: 05/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG15 Species: Caenorhabditis elegans Genotype: emb-15(g15) X. Description: Temperature sensitive. Maintain at 15C. Will also grow at 20C, but not 25C. Mutagen: EMS Outcrossed: x Made by: Received: 08/05/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG16 Species: Caenorhabditis elegans Genotype: emb-5(g16) III. Description: Temperature sensitive. Maintain at 15C. At 25C the embryos arrest in late morphogenesis. Mutagen: EMS Outcrossed: x Made by: Received: 05/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG19 Species: Caenorhabditis elegans Genotype: div-1(g19) III. Description: Maintain at 15C. Temperature sensitive. Will grow at 20C, but does better at 15C (viable, somewhat Unc). At 25C: 91% of eggs arrest at lima bean. Mutagen: EMS Outcrossed: x Made by: Received: 08/19/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG20 Species: Caenorhabditis elegans Genotype: emb-17(g20) I. Description: ts. Maintain at 15C. Some growth at 20C. Mutagen: EMS Outcrossed: x Made by: Received: 05/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG201 Species: Caenorhabditis elegans Genotype: ace-2(g72) I; ace-1(p1000) X. Description: Slow moving Unc. Cannot back. Hypercontracts when you tap nose. Mutagen: EMS Outcrossed: x2 Made by: Culotti J Received: 02/01/82 Mt. Sinai Hospital Research Institute, Toronto, Ontario ----------------------------------------------------------------------------- Strain: GG202 Species: Caenorhabditis elegans Genotype: ace-2(g72) I. Description: Phenotypically WT. AChE ACTIVITY :SENS TO 0.1% TRITON X-100. Mutagen: EMS Outcrossed: x Made by: Culotti J Received: 05/01/81 Mt. Sinai Hospital Research Institute, Toronto, Ontario ----------------------------------------------------------------------------- Strain: GG21 Species: Caenorhabditis elegans Genotype: emb-18(g21) V. Description: Temperature sensitive. Maintain at 15C. Embryonic lethal at 25C. Grows at 20C. Mutagen: EMS Outcrossed: x Made by: Received: 08/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG22 Species: Caenorhabditis elegans Genotype: emb-19(g22) I. Description: Temperature sensitive. Maintain at 15C. Some growth at 20C. Mutagen: EMS Outcrossed: x Made by: Received: 05/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG23 Species: Caenorhabditis elegans Genotype: emb-9(g23) III. Description: Temperature sensitive. Maintain at 15C, will not grow at 25C. Mutagen: EMS Outcrossed: x Made by: Received: 05/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG25 Species: Caenorhabditis elegans Genotype: let-2(g25) X. Description: Temperature sensitive. Maintain at 15C. See also CGC 1806. Mutagen: EMS Outcrossed: x Made by: Received: 05/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG27 Species: Caenorhabditis elegans Genotype: emb-20(g27) I. Description: ts. Maintain at 15C. Some growth at 20C. 96% Emb at 25.6C and 25.8C. Mutagen: EMS Outcrossed: x Made by: Received: 05/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG30 Species: Caenorhabditis elegans Genotype: let-2(g30) X. Description: Temperature sensitive. Maintain at 15C. No growth at 20C. See also CGC 1806. Mutagen: EMS Outcrossed: x Made by: Received: 05/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG31 Species: Caenorhabditis elegans Genotype: emb-21(g31) II. Description: Temperature sensitive. Maintain at 15C. Will grow at 20C, but not at 25C. [11/93: Not behaving as described. Very sick at 25C, but giving some live offspring which in turn give a few more live offspring.] Mutagen: EMS Outcrossed: x Made by: Received: 08/05/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG32 Species: Caenorhabditis elegans Genotype: emb-22(g32) V. Description: Temperature sensitive. Maintain at 15C. Embryonic lethal at 25C. Some growth at 20C. Mutagen: EMS Outcrossed: x Made by: Received: 09/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG34 Species: Caenorhabditis elegans Genotype: emb-9(g34) III. Description: Temperature sensitive. Maintain at 15C. At 25C the animals die as pretzels; a few hatch and die as L1. Will grow at 20C. Mutagen: EMS Outcrossed: x Made by: Received: 05/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG36 Species: Caenorhabditis elegans Genotype: tyms-1(g36) I. Description: Temperature sensitive. Maintain at 15C. Will grow at 20C, but not 25C. Mutagen: EMS Outcrossed: x Made by: Received: 05/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG37 Species: Caenorhabditis elegans Genotype: let-2(g37) X. Description: Temperature sensitive. Maintain at 15C. See also WBPaper00001806. Mutagen: EMS Outcrossed: x Made by: Received: 05/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG38 Species: Caenorhabditis elegans Genotype: mett-10(g38) III. Description: Maternal effect temperature sensitive embryonic lethal. Leaky. Maintain at 15C. mett-10 was formerly known as let-42. Mutagen: Outcrossed: x Made by: Received: 04/14/95 Washington University School of Medicine, St. Louis MO, USA ----------------------------------------------------------------------------- Strain: GG39 Species: Caenorhabditis elegans Genotype: emb-23(g39) II. Description: Temperature sensitive. Maintain at 15C. Embryonic lethal.(Some growth at 20C.) [11/93: Not behaving as described. Very sick at 25C, but giving some live offspring which in turn give a few more live offspring.] Mutagen: EMS Outcrossed: x Made by: Received: 08/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG4 Species: Caenorhabditis elegans Genotype: emb-11(g4) IV. Description: Temperature sensitive. Maintain at 15C. Some growth at 20C and 25.4C--very leaky. Mutagen: EMS Outcrossed: x Made by: Received: 05/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG40 Species: Caenorhabditis elegans Genotype: emb-24(g40) III. Description: Temperature sensitive. Accumulates dead eggs at permissive temperature (15C). Will grow at 20C, but not 25C. [5/95: Not tight at 25C - a few embryos are surviving and reproducing.] Mutagen: EMS Outcrossed: x Made by: Received: 10/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG43 Species: Caenorhabditis elegans Genotype: fasn-1(g43) I. Description: Temperature sensitive. Maintain at 15C. Some growth at 20C. Mutagen: EMS Outcrossed: x Made by: Received: 05/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG45 Species: Caenorhabditis elegans Genotype: emb-25(g45) III. Description: Temperature sensitive. Maintain at 15C. Some growth at 20C. Mutagen: EMS Outcrossed: x Made by: Received: 08/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG47 Species: Caenorhabditis elegans Genotype: emb-26(g47) IV. Description: Temperature sensitive, maintain at 15C. At 25.6C 80% of the eggs arrest at lima bean and have abnormal gut granule birefringence. Leaky at 25C. Will grow somewhat at 20C also. Mutagen: EMS Outcrossed: x Made by: Received: 08/19/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG48 Species: Caenorhabditis elegans Genotype: emb-27(g48) II. Description: ts. Maintain at 15C. Some growth at 20C. Mutagen: EMS Outcrossed: x Made by: Received: 05/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG49 Species: Caenorhabditis elegans Genotype: emb-28(g49) V. Description: Temperature sensitive. Maintain at 15C. Will grow at 20C, but not at 25C. Mutagen: EMS Outcrossed: x Made by: Received: 08/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG5 Species: Caenorhabditis elegans Genotype: emb-12(g5) I. Description: ts. Maintain at 15C. (Some growth at 20C and 25.4C. 72% Emb at 25.6C and 25.8C) Mutagen: EMS Outcrossed: x Made by: Received: 05/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG52 Species: Caenorhabditis elegans Genotype: cdc-25.2(g52) V. Description: Temperature sensitive. Maintain at 15C. 100% arrest at 25C. Some growth at 20C. Mutagen: EMS Outcrossed: x Made by: Received: 10/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG53 Species: Caenorhabditis elegans Genotype: emb-30(g53) III. Description: Temperature sensitive. Maintain at 15C. Will also grow at 20C, but not 25C. Mutagen: EMS Outcrossed: x Made by: Received: 07/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG55 Species: Caenorhabditis elegans Genotype: emb-31(g55) IV. Description: Temperature sensitive, maintain at 15C. At 25C the eggs arrest at lima bean and have normal gut granule birefringence. Will grow at 20C. Mutagen: EMS Outcrossed: x Made by: Received: 08/19/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG57 Species: Caenorhabditis elegans Genotype: mus-101(g57) III. Description: ts embryonic lethal. Grows at 15C. Mutagen: EMS Outcrossed: x Made by: Received: 05/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG58 Species: Caenorhabditis elegans Genotype: emb-32(g58) III. Description: Temperature sensitive. Maintain at 15C. Will grow at 20C, but not 25C. Mutagen: EMS Outcrossed: x Made by: Received: 08/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG6 Species: Caenorhabditis elegans Genotype: emb-13(g6) III. Description: Temperature sensitive. Grow at 15C. Some growth at 20C. Does not grow at 25C. Mutagen: EMS Outcrossed: x Made by: Received: 09/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG60 Species: Caenorhabditis elegans Genotype: glp-1(g60) III. Description: Accumulates dead eggs at permissive temperature (15C). Will grow at 20C, but not at 25C. g60 pka emb-33. Mutagen: EMS Outcrossed: x Made by: Received: 08/05/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG62 Species: Caenorhabditis elegans Genotype: emb-34(g62) III. Description: Temperature sensitive-maintain at 15C. Some growth at 20C. Does not grow at 25C. Mutagen: EMS Outcrossed: x Made by: Received: 08/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG64 Species: Caenorhabditis elegans Genotype: emb-35(g64) IV. Description: Temperature sensitive. Maintain at 15C. Embryonic lethal at 25C. Mutagen: EMS Outcrossed: x Made by: Received: 08/19/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GG65 Species: Caenorhabditis elegans Genotype: emb-5(g65) III. Description: Temperature sensitive. Maintain at 15C. Will grow at 20C, but not at 25C. Mutagen: EMS Outcrossed: x Made by: Received: 05/01/81 Max-Planck Institut, Gottingen, Germany ----------------------------------------------------------------------------- Strain: GH378 Species: Caenorhabditis elegans Genotype: pgp-2(kx48) I. Description: Reduced number of birefringent gut granules in embyros and a mislocalization of birefringence to the lumen. Faint autofluorescent gut granules in adults. Mutagen: EMS Outcrossed: x5 Made by: Lena Schroeder Received: 06/22/07 Lewis and Clark College, Portland, OR ----------------------------------------------------------------------------- Strain: GH383 Species: Caenorhabditis elegans Genotype: glo-3(zu446) X. Description: Class II allele. Partial loss of gut granules (birefringence) in intestinal cells and low penetrance of mislocalized birefringent material into the intestinal lumen. Reference: Rabbitts et al. (2008) Genetics 180:857-871. Mutagen: Outcrossed: x Made by: Greg Hermann Received: 07/13/10 Lewis and Clark College, Portland, OR ----------------------------------------------------------------------------- Strain: GH403 Species: Caenorhabditis elegans Genotype: glo-3(kx94) X. Description: Class I allele. Complete loss of gut granules (birefringence) in intestinal cells and mislocalization of birefringent material into the intestinal lumen. Reference: Rabbitts et al. (2008) Genetics 180:857-871. Mutagen: Outcrossed: x Made by: Greg Hermann Received: 07/13/10 Lewis and Clark College, Portland, OR ----------------------------------------------------------------------------- Strain: GH534 Species: Caenorhabditis elegans Genotype: mrp-4(cd8) X. Description: WT strain. cd8 will suppress the embryonic and larval lethality of cup-5(zu223). Mutagen: EMS Outcrossed: x4 Made by: Received: 03/26/07 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: GH636 Species: Caenorhabditis elegans Genotype: umps-1(zu456) III. Description: Reference: Levitte S, et al. FEBS J. 2010 Mar;277(6):1420-39. Mutagen: EMS Outcrossed: x6 Made by: Greg Hermann Received: 06/13/11 Lewis and Clark College, Portland, OR ----------------------------------------------------------------------------- Strain: GIN101 Species: Caenorhabditis elegans Genotype: thoc-2(tm1310) III/hT2[bli-4(e937) let-?(q782) qIs48] (I;III). Description: Homozygous sterile allele balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP sterile homozygotes (tm1310 is maternally rescued; progeny of non-GFP animals fail to give viable progeny). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Castellano-Pozo, García-Muse T, Aguilera A. 2012 R-loops cause replication impairment and genome instability during meiosis. EMBO Rep. 13(10): 923-9. Mutagen: UV/TMP Outcrossed: x7 Made by: M. Castellano-Pozo Received: 12/02/14 CAMIMER, Sevilla, Spain ----------------------------------------------------------------------------- Strain: GIN102 Species: Caenorhabditis elegans Genotype: thoc-2(tm1310) III/hT2[bli-4(e937) let-?(q782) qIs48] (I;III); spo-11(ok79) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Maintain by picking Unc GFP progeny that produce viable embryos and checking that the non-GFP progeny that are produced fail to give viable progeny. tm1310 is a homozygous sterile allele balanced by bli-4- and GFP-marked translocation. Heterozygotes are Unc with pharyngeal GFP signal, and segregate Unc GFP, arrested hT2 aneuploids, and non-GFP sterile homozygotes (tm1310 is maternally rescued; progeny of non-GFP animals fail to give viable progeny). Homozygous hT2 [bli-4 let-? qIs48] inviable. ok79 heterozygotes are Unc and segregate Uncs, dead eggs, and Him ok79 homozygotes (maternally rescued). Reference: Castellano-Pozo, García-Muse T, Aguilera A. 2012 R-loops cause replication impairment and genome instability during meiosis. EMBO Rep. 13(10): 923-9. Mutagen: UV/TMP Outcrossed: x Made by: M. Castellano-Pozo Received: 12/02/14 CAMIMER, Sevilla, Spain ----------------------------------------------------------------------------- Strain: GJ20 Species: C. elegans Genotype: dpy-20(e1282) IV; pkIs1201. Description: pkIs1201 [gpa-3::GFP + dpy-20(+)]. Mutagen: N/A Outcrossed: x0 Made by: Plasterk lab Received: 09/25/23 Erasmus University, Rotterdam, The Netherlands ----------------------------------------------------------------------------- Strain: GJ3452 Species: C. elegans Genotype: gcy-22(gj1976[gcy-22::GFP]) V. Description: GFP tag inserted into endogenous gcy-22 locus. GCY-22::GFP expression in the ASER neuron localized at cilium tip and PCMC. Reference: van der Burght SN, et al. Curr Biol. 2020 Nov 2;30(21):4299-4306.e5. PMID: 32916106 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Servaas N. van der Burght Received: 04/11/22 Erasmus University, Rotterdam, The Netherlands ----------------------------------------------------------------------------- Strain: GJ443 Species: C. elegans Genotype: gjIs140. Description: gjIs140 [gpa-4::GFP + dpy-20(+)]. Reporter construct includes 2.5 kb upstream and the first exon of gpa-4 fused in frame with GFP. Reference: Burghoorn et al. Proc. Natl. Acad. Sci. USA 2007 Apr; 104(17):7157-62. Mutagen: N/A Outcrossed: x0 Made by: Gert Jansen lab Received: 09/25/23 Erasmus University, Rotterdam, The Netherlands ----------------------------------------------------------------------------- Strain: GJ7 Species: Caenorhabditis elegans Genotype: gpa-2(pk16) gpa-3(pk35) gpa-13(pk1270) V; gpa-5(pk376) gpa-6(pk480) X. Description: Lans H, et al. Genetics. 2004 Aug;167(4):1677-87. Mutagen: Outcrossed: x0 Made by: Gert Jansen Received: 09/29/14 Erasmus University, Rotterdam, The Netherlands ----------------------------------------------------------------------------- Strain: GKC1 Species: C elegans Genotype: mip-1(uae1) III. Description: Temperature-sensitive sterile; maintain at 15-20C. uae1 is a CRISPR-engineered deletion of the mip-1 coding region. Reference: Cipriani PG. et al. Elife. 2021 Jul 5;10:e60833. doi: 10.7554/eLife.60833. Mutagen: Outcrossed: x4 Made by: Hala Fahs Received: 12/16/21 New York University, New York, NY ----------------------------------------------------------------------------- Strain: GKC2 Species: C elegans Genotype: mip-2(uae2) V. Description: Temperature-sensitive sterile; maintain at 15-20C. uae2 is a CRISPR-engineered deletion of the mip-2 coding region. Reference: Cipriani PG. et al. Elife. 2021 Jul 5;10:e60833. doi: 10.7554/eLife.60833. Mutagen: Outcrossed: x4 Made by: Hala Fahs Received: 12/16/21 New York University, New York, NY ----------------------------------------------------------------------------- Strain: GL302 Species: Caenorhabditis elegans Genotype: cid-1(rf34) III. Description: K10D2.3 Slow development. Reduced fertility. Thermotolerant. Resistant to hydroxyurea. Mutagen: Tc4 Outcrossed: x2 Made by: Anders Olsen Received: 07/14/06 Buck Institute, Novato CA, USA ----------------------------------------------------------------------------- Strain: GL347 Species: Caenorhabditis elegans Genotype: zcIs13 V. Description: zcIs13 [hsp-6p::GFP + lin-15(+)] V. Stable transgenic line with GFP expression mainly in the posterior intestine, observed from L1 to adult. Perturbation of mitochondrial folding environment induces robust GFP expression throughout the intestines. Derived by outcrossing parental strain SJ4100 to N2 six times to remove suspected background mutations causing uneven transgene expression and low penetrance of sterility. Mutagen: Outcrossed: x6 Made by: Suzanne Angeli Received: 09/28/21 Buck Institute, Novato CA, USA ----------------------------------------------------------------------------- Strain: GL390 Species: C. elegans Genotype: aco-2(rf40[aco-2::GFP]) III. Description: C-terminal GFP tag inserted into endogenous aco-2 locus. ACO-2::GFP is a mitochondrial marker allowing the examination of native mitochondrial morphology in live animals. Reference: Begelman DV, et al. 2022 Jul 17;2022:10.17912/micropub.biology.000599. doi: 10.17912/micropub.biology.000599. eCollection 2022. PMID: 35903774 Mutagen: Crispr/Cas9 Outcrossed: x6 Made by: Dipa Bhaumik Received: 12/07/22 Buck Institute, Novato CA, USA ----------------------------------------------------------------------------- Strain: GLW16 Species: C. elegans Genotype: rab-7(utx12[mNG::rab-7]) II. Description: Superficially wild-type. N-terminal tag of RAB-7 via CRISPR/Cas9 knock-in of mNeonGreen at rab-7 locus. Insertion verified by PCR. Left flank: 5' gcacaacaaaaaggcttccagtgaacaaaa 3'; Right flank: 5' ATGTCGGGAACCAGAAAGAAGGCGCTGCTC 3'. sgRNA: 5' cttccagtgaacaaaaATGT 3' Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Bailey de Jesus (Glow Worms '21) Received: 09/09/21 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW19 Species: C. elegans Genotype: mpk-1(utx14[mNG::mpk-1]) III. Description: Superficially wild-type. N-terminal tag of MPK-1 via CRISPR/Cas9 knock-in of mNeonGreen at mpk-1 locus. Insertion verified by PCR. Left flank: 5' tagaaatttaaaattcatttcttcttgcag 3'; Right flank: 5' ATGGCCGACGGAGAAGCGGTTATCTCGACG 3'. sgRNA: 5' ttcttcttgcagATGGCCGA 3' Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Alex Koh (Glow Worms '20) Received: 09/09/21 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW2 Species: C. elegans Genotype: attf-2(utx2[mNG::attf-2]) V. Description: Superficially wild-type. N-terminal tag of ATTF-2 via CRISPR/Cas9 knock-in of mNeonGreen at attf-2 locus. Insertion verified by PCR. Left flank: 5' cttttttgctcacatcatcatttttcagtc 3'; Right flank: 5' ATGTCGGCCGAGACCGCGACTATCCCCGAAGTTTC 3' (5 silent mutations). sgRNA: 5' CGAAACTGCAACCATACCCG 3' Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: George Huang (Glow Worms '20) Received: 07/26/21 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW25 Species: Caenorhabditis elegans Genotype: daf-18(utx19[mNG::3xFlag::daf-18]) IV. Description: Superficially wild type. N-terminal tag of DAF-18 via CRISPR/Cas9 knock-in of mNeonGreen at daf-18 locus. Insertion verified by PCR and fluorescence. Left flank: 5' gcagtttccaggtacatctactaaccccca 3'; Right flank: 5' ATGGTTACTCCTCCTCCAGATGTGCCAAGC 3'; sgRNA: GGAGGAGGAGTAACCATtgg; Cas9/sgRNA plasmid: pGLOW27; mNG^SEC^3xFlag plasmid: pGLOW53; SEC insertion allele strain: GLW24. Reference: Huang et al. 2021. Improved CRISPR/Cas9 knock-in efficiency via the self-excising cassette (SEC) selection method in C. elegans. 2021 Sep 16;2021:10.17912/micropub.biology.000460. doi: 10.17912/micropub.biology.000460. eCollection 2021. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Amaka Okafor (Glow Worms '20) Received: 11/22/21 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW27 Species: Caenorhabditis elegans Genotype: muIs252 II; unc-119(ed3) his-72(utx21[his-72::wrmScarlet11::3xMyc]) III. Description: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. C-terminal tag of HIS-72 via CRISPR/Cas9 knock-in of wrmScarlet11 into endogenous his-72 locus. Genetic background: strain CF4582. Insertion verified by PCR and fluorescence. Left flank: 5' CTCGCCAGACGCATTCGCGGAGAACGTGCT 3' (one silent mutation); Right flank: 5' TAAgctccatcaccaattctcgaagcactt 3'; sgRNA: GAGCTTAAGCACGTTCTCCG; Cas9/sgRNA plasmid: pGLOW87; wrmScarlet11^SEC^3xMyc plasmid: pGLOW88; SEC insertion allele strain: GLW26 Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Ella DeMott (Glow Worms '20) Received: 12/21/21 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW29 Species: Caenorhabditis elegans Genotype: muIs252 II; unc-119(ed3) III; egl-1(utx23[egl-1::wrmScarlet11::3xMyc]) V. Description: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. C-terminal tag of EGL-1 via CRISPR/Cas9 knock-in of wrmScarlet11 into endogenous egl-1 locus. Genetic background: strain CF4582. Insertion verified by PCR, Sanger sequencing, and fluorescence. Left flank: 5' CAGAAGTCTCTTCCATCGTCTTCTGGACTTTTTCGCTTTT 3' (one silent mutation); Right flank: 5' TAAgtgatcaaaatctccaacttttctcca 3'; sgRNA: AGTCCAGAAGACGATGGAAG; Cas9/sgRNA plasmid: pGLOW65; wrmScarlet11^SEC^3xMyc plasmid: pGLOW66; SEC insertion allele strain: GLW28. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Ella DeMott (Glow Worms '20) Received: 12/21/21 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW33 Species: Caenorhabditis elegans Genotype: T28D6.6(utx25[T28D6.6::mNG::3xFlag]) III. Description: Superficially wild type. C-terminal tag of T28D6.6 via CRISPR/Cas9 knock-in of mNeonGreen at T28D6.6 locus. Insertion verified by PCR and fluorescence. Left flank: 5' gtcgcaaataatggttttttttccagAGTC 3'; Right flank: 5' TAAgctgaaattcccgtgcttctcgtcttc 3'; sgRNA: gggaatttcagcTTAGACTc; Cas9/sgRNA plasmid: pGLOW2; mNG^SEC^3xFlag plasmid: pGLOW42; SEC insertion allele strain: GLW32. Reference: Huang et al. 2021. Improved CRISPR/Cas9 knock-in efficiency via the self-excising cassette (SEC) selection method in C. elegans. 2021 Sep 16;2021:10.17912/micropub.biology.000460. doi: 10.17912/micropub.biology.000460. eCollection 2021. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Skye Waterland (Glow Worms '20) Received: 11/22/21 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW35 Species: Caenorhabditis elegans Genotype: gldi-2(utx27[mNG::3xFlag::T13C2.6]) II. Description: T13C2.6. N-terminal tag of T13C2.6 via CRISPR/Cas9 knock-in of mNeonGreen at gldi-2 locus. Insertion verified by PCR and fluorescence. Left flank: 5' aaatattaattgataatcagaggagtaaaa 3'; Right flank: 5' ATGAGGACATGTCTCACCCTCACGGGTTTC 3'; sgRNA: taatcagaggagtaaaaATG; Cas9/sgRNA plasmid: pGLOW45; mNG^SEC^3xFlag plasmid: pGLOW54; SEC insertion allele strain: GLW34. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Staci Guillen (Glow Worms '21) Received: 12/21/21 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW39 Species: Caenorhabditis elegans Genotype: ccdc-47(utx31[mNG::3xFlag::ccdc-47]) III. Description: N-terminal tag of CCDC-47 via CRISPR/Cas9 knock-in of mNeonGreen at ccdc-47 locus. Insertion verified by PCR, Sanger sequencing, and fluorescence. Left flank: 5' gttaaatcactcaatttcgggtcgttcacc 3'; Right flank: 5' ATGAAGATAGTATGGATTTTCCTAATATTC 3' (3 silent mutations); sgRNA: cgttcaccATGAAAATCGTC; Cas9/sgRNA plasmid: pGLOW13; mNG^SEC^3xFlag plasmid: pGLOW17; SEC insertion allele strain (balanced): GLW38. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Anita Rhodes (Glow Worms '20) Received: 12/21/21 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW4 Species: C. elegans Genotype: gyf-1(utx4[gyf-1::mNG]) II. Description: Superficially wild-type. C-terminal tag of GYF-1 via CRISPR/Cas9 knock-in of mNeonGreen at gyf-1 locus. Insertion verified by PCR. Left flank: 5' CCATCGGCTCCGGTGAATCCTTCGCGCCGT 3'; Right flank: 5' TAGatgagtcatttctttttccagctttaa 3'. sgRNA: 5' TGACTCATCTAACGGCGCGA 3' Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Sara Blanco (Glow Worms '20) Received: 07/26/21 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW41 Species: Caenorhabditis elegans Genotype: nhr-8(utx33[mNG::3xFlag::nhr-8]) IV. Description: N-terminal tag of NHR-8 via CRISPR/Cas9 knock-in of mNeonGreen at nhr-8 locus. Insertion verified by PCR and Sanger sequencing. Left flank: 5' taatcactaaaacaaaaatttcgtcattcc 3'; Right flank: 5' ATGCCTTCGTCTTCTCCATCGATGGACGAG 3'; sgRNA: CGATGGAGAAGACGAAGGCA; Cas9/sgRNA plasmid: pGLOW55; mNG^SEC^3xFlag plasmid: pGLOW56; SEC insertion allele strain: GLW40. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Gillian Witten (Glow Worms '21) Received: 12/21/21 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW43 Species: Caenorhabditis elegans Genotype: edc-3(utx35[mNG::3xFlag::edc-3]) I. Description: N-terminal tag of EDC-3 via CRISPR/Cas9 knock-in of mNeonGreen at edc-3 locus. Insertion verified by PCR and fluorescence. Left flank: 5' ggttccaattcttctgatttcaaattaaaa 3'; Right flank: 5' ATGGATGACAAACTCATTGGAAGCGTCATCTCTACGGAGACAAAGGACGG 3' (7 silent mutations); sgRNA: ATATCAACCGAAACTAAAGA; Cas9/sgRNA plasmid: pGLOW81; mNG^SEC^3xFlag plasmid: pGLOW103; SEC insertion allele strain: GLW42. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Diya Sharma (Glow Worms '21) Received: 01/24/22 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW45 Species: Caenorhabditis elegans Genotype: ZK1058.9(utx37[ZK1058.9::mNG::3xFlag]) III Description: C-terminal tag of ZK1058.9 via CRISPR/Cas9 knock-in of mNeonGreen at ZK1058.9 locus. Insertion verified by PCR and fluorescence. Left flank: 5' AGCTCAACGGCTAGCTGGTCTCGTTATTAT 3' (1 silent mutation); Right flank: 5' TAAtgaattttcctccaacttttgtcctct 3'; sgRNA: AATAACGAGACCAGCTAG (18 bp); Cas9/sgRNA plasmid: pGLOW58; mNG^SEC^3xFlag plasmid: pGLOW68; SEC insertion allele strain: GLW44. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Dan Tatulescu (Glow Worms '21) Received: 01/24/22 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW47 Species: Caenorhabditis elegans Genotype: hsp-4(utx39[hsp-4::mNG::3xFlag]) II. Description: C-terminal tag of HSP-4 via CRISPR/Cas9 knock-in of mNeonGreen at hsp-4 locus. Insertion verified by PCR and fluorescence. Left flank: 5' TCGGCCGGAGGACAAGGAGAACAAGCTTCTGAGGAGCCATCGGAGGATCATGATGAACTG 3' (1 silent mutation); Right flank: 5' TAAaatattaattgccttcaactacttgct 3'; sgRNA: CGTCTCCAAACTTTACTCGG; Cas9/sgRNA plasmid: pGLOW44; mNG^SEC^3xFlag plasmid: pGLOW61; SEC insertion allele strain: GLW46. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Quincy Martin (Glow Worms '21) Received: 01/24/22 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW51 Species: Caenorhabditis elegans Genotype: cey-1(utx43[mNG::3xFlag::cey-1]) II. Description: N-terminal tag of CEY-1 via CRISPR/Cas9 knock-in of mNeonGreen at cey-1 locus. Insertion verified by PCR and fluorescence. Left flank: 5' accagaggaagaacagaagcgcgcggaaca 3'; Right flank: 5' ATGGCGGAGAAAAACgtaagtgtgtgtctc 3' (1 silent mutation); sgRNA: acacacacttacGTTTTTCT; Cas9/sgRNA plasmid: pGLOW71; mNG^SEC^3xFlag plasmid: pGLOW93; SEC insertion allele strain (balanced): GLW50. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Liam Schuck (Glow Worms '21) Received: 02/16/22 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW53 Species: Caenorhabditis elegans Genotype: egl-19(utx45[egl-19(A&B)::mScarlet-I-C1::3xMyc] IV. Description: Internal tag of EGL-19 at N-terminal side of exon 3 via CRISPR/Cas9 knock-in of mScarlet at egl-19 locus. Tags isoforms a and b. Insertion verified by PCR and fluorescence. Left flank: 5' ttatttgaatgagcaaaaaataaatttcag 3'; Right flank: 5' GCCGCAGTGGCAGCTTCATCATCACAAGAT 3’ (1 silent mutation); gRNA: TTGTGATGATGAAGCTGCCA; Cas9/sgRNA plasmid: pGLOW69; mScarlet^SEC^3xMyc plasmid: pGLOW60; SEC insertion allele strain (balanced): GLW52. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Kara McDonald (Glow Worms '21) Received: 01/10/23 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW57 Species: Caenorhabditis elegans Genotype: asp-3(utx47[mNG::3xFlag::asp-3]) X. Description: N-terminal tag of ASP-3 via CRISPR/Cas9 knock-in of mNeonGreen at asp-3 locus. Insertion verified by PCR and fluorescence. Left flank: 5' gcgctgcttctcaattagtgataacgcacc 3'; Right flank: 5' ATGTCGGGCCGCGTTTTCCTTCTTCTGGCT 3'; sgRNA: tagtgataacgcaccATGT (19 bp); Cas9/sgRNA plasmid: pGLOW74; mNG^SEC^3xFlag plasmid: pGLOW96; SEC insertion allele strain: GLW58. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Stephen Pullman (Glow Worms '21) Received: 04/27/22 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW6 Species: C. elegans Genotype: W08E12.7(utx6[mNG::W08E12.7]) IV. Description: Superficially wild-type. N-terminal tag of W08E12.7 via CRISPR/Cas9 knock-in of mNeonGreen at W08E12.7 locus. Insertion verified by PCR. Left flank: 5' ttcaatgtttattctttcagatagatcaaa 3'; Right flank: 5' ATGGTTAGAAAGGACAGTGAGAGTAGTTGTTCAAGTGATGG 3' (6 silent mutations). sgRNA: 5' GAAAGCAGCTGCTCCAGCGA 3' Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Aubrie Rettmann (Glow Worms '20) Received: 07/26/21 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW63 Species: Caenorhabditis elegans Genotype: pqn-59(utx51[mNG::3xFlag::pqn-59]) I. Description: N-terminal tag of PQN-59 via CRISPR/Cas9 knock-in of mNeonGreen at pqn-59 locus. Insertion verified by PCR and fluorescence. Left flank: 5' gaccctttttatttataatttgttttcaga 3'; Right flank: 5' ATGGGTATTAAAGGCGACAAAAAAGCTACT 3’ (1 silent mutation); sgRNA: TGCAAGTCGCGCTTGATCGC; Cas9/sgRNA plasmid: pGLOW23; mNG^SEC^3xFlag plasmid: pGLOW24; SEC insertion allele strain (balanced): GLW62. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Persephone Alicea (Glow Worms '20) Received: 04/27/22 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW65 Species: Caenorhabditis elegans Genotype: sod-2(utx53[mScarlet::3xMyc::sod-2]) I. Description: N-terminal tag of SOD-2 via CRISPR/Cas9 knock-in of mScarlet at sod-2 locus. Insertion verified by PCR and fluorescence. Left flank: 5' attgaatattttaattatttgcagccgaaa 3'; Right flank: 5' ATGCTTCAAAACACGGTTCGCTGTGTCTCA 3’ (1 silent mutation); sgRNA: AAGCTTTGAGACACAGCGAA; Cas9/sgRNA plasmid: pGLOW98; mScarlet^SEC^3xMyc plasmid: pGLOW111; SEC insertion allele strain: GLW64. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Param Kapoor (Glow Worms '21) Received: 04/27/22 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW73 Species: Caenorhabditis elegans Genotype: H34C03.2(utx55[mNG::3xFlag::H34C03.2]) IV. Description: N-terminal tag of H34C03.2 via CRISPR/Cas9 knock-in of mNeonGreen at H34C03.2 locus. Insertion verified by PCR and fluorescence. Left flank: 5' gggattgcctacactcaaatatacgtaacg 3'; Right flank: 5' ATGCCCACGCCGGAAGTGTTCCCATGGATG 3’ (4 silent mutations); gRNA: cgtaacgATGCCAACTCCCG; Cas9/sgRNA plasmid: pGLOW9; mNG^SEC^3xFlag plasmid: pGLOW106; SEC insertion allele strain: GLW72. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Christine Collins, Vinay Pillai, Wesley Yeung Received: 01/10/23 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW77 Species: Caenorhabditis elegans Genotype: jnk-1(utx59[mNG::3xFlag::jnk-1]) IV. Description: N-terminal tag of JNK-1 via CRISPR/Cas9 knock-in of mNeonGreen at jnk-1 locus. Insertion verified by PCR and fluorescence. Left flank: 5' cagcaagttaaagtgtgtgatcctgtgcac 3'; Right flank: 5' ATGGAGGAACGATTATCCACAACATCATCG 3’; gRNA: gtgtgatcctgtgcacATGG; Cas9/sgRNA plasmid: pGLOW114a; mNG^SEC^3xFlag plasmid: pGLOW116; SEC insertion allele strain: GLW76. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Kennedy Current (Glow Worms '21) Received: 01/10/23 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW79 Species: Caenorhabditis elegans Genotype: sod-5(utx61[mScarlet-I-C1::3xMyc::sod-5]) II. Description: N-terminal tag of SOD-5 via CRISPR/Cas9 knock-in of mScarlet at sod-5 locus. Insertion verified by PCR and fluorescence. Left flank: 5' tgtgctaacgaaaattttactaaaaggaaa 3'; Right flank: 5' ATGGATATTCTCTCTGATATTGCAAATGCC 3’ (1 silent mutation); sgRNA: tacCTGTGGAAGAACGGCAT; Cas9/sgRNA plasmid: pGLOW122; mScarlet^SEC^3xMyc plasmid: pGLOW129; SEC insertion allele strain: GLW78. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Gaby Sobrevilla (Glow Worms '22) Received: 01/10/23 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW8 Species: C. elegans Genotype: eel-1(utx8[mNG::eel-1]) IV. Description: Slow growing. N-terminal tag of EEL-1 via CRISPR/Cas9 knock-in of mNeonGreen at eel-1 locus. Insertion verified by PCR. Left flank: 5' gacaagcgggttttttgaatcgtttcgaaa 3'; Right flank: 5' ATGAAGATTGACGATGCTGAGCCGAGCTCTAGTTCATCGGGCTCCGATAT 3' (6 silent mutations). sgRNA: 5' GACCCGGAAGAGCTTGAACT 3' Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Ella DeMott (Glow Worms '20) Received: 09/09/21 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW85 Species: Caenorhabditis elegans Genotype: pqe-1(utx65[mScarlet-I-C1::3xMyc::pqe-1]) III. Description: N-terminal tag of PQE-1 via CRISPR/Cas9 knock-in of mScarlet at pqe-1 locus. Insertion verified by PCR and fluorescence. Genotyping primers: fwd – 5’ CCAGATGCGAAAGCGAACAG 3’ ; rev – 5’ TGTACTTACCGGAATCGGCG 3’. Left flank: 5' ttaataatcattccagcaagtatctcagcc 3'; Right flank: 5' ATGTTTAATGGCGGCTATGGATCTGGAAAC 3’; sgRNA: atctcagccATGTTTAATGG; Cas9/sgRNA plasmid: pGLOW143; mScarlet^SEC^3xMyc plasmid: pGLOW142; SEC insertion allele strain: GLW84. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Ann Houghton (Glow Worms '22) Received: 01/10/23 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW89 Species: Caenorhabditis elegans Genotype: ubql-1(utx69[mScarlet-I-C1::3xMyc::ubql-1]) I. Description: N-terminal tag of UBQL-1 via CRISPR/Cas9 knock-in of mScarlet at ubql-1 locus. Insertion verified by PCR and fluorescence. Genotyping primers: fwd – 5’ AGGGCGAGAGATTATCGGGA 3’; rev – 5’ CGGATCGTTGAGAATGTGTCC 3’. Left flank: 5' gtcggttttttaatatttctcaaatttaag 3'; Right flank: 5' ATGGCTACAGAGAGTGCACTCATCAAAGTTCACGTGAAATCACCCT 3’ (7 silent mutations); sgRNA: TCAACGTCATACTTGTTCGA; Cas9/sgRNA plasmid: pGLOW134; mScarlet^SEC^3xMyc plasmid: pGLOW145; SEC insertion allele strain: GLW88. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Frankie Zelasko (Glow Worms '22) Received: 01/10/23 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW91 Species: Caenorhabditis elegans Genotype: hrpa-1(utx71[hrpa-1::mScarlet-I-C1::3xMyc]) IV. Description: C-terminal tag of HRPA-1 via CRISPR/Cas9 knock-in of mScarlet at hrpa-1 locus. Insertion verified by PCR and fluorescence. Genotyping primers: fwd – 5’ GTCTCCACCAAACGCCTGTA 3’ ; rev – 5’ CTAGCCTGTGTCCCATCAGC 3’. Left flank: 5' AATGGGCCCACGCCCAGGGAGGAAACAGAAACTAT 3' (5 silent mutations); Right flank: 5' TAAattaattccttaagcccctctaagtgt 3’; sgRNA: CAGTGGGCTCATGCTCAAGG; Cas9/sgRNA plasmid: pGLOW144; mScarlet^SEC^3xMyc plasmid: pGLOW153; SEC insertion allele strain: GLW90. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Ayla Saeed (Glow Worms '22) Received: 01/10/23 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW93 Species: Caenorhabditis elegans Genotype: clic-1(utx73[mScarlet-I-C1::3xMyc::clic-1]) V. Description: N-terminal tag of CLIC-1 via CRISPR/Cas9 knock-in of mScarlet at clic-1 locus. Insertion verified by PCR and fluorescence. Genotyping primers: fwd – 5’ CTCGTACCAATCGTCCGCAA 3’; rev – 5’ CCATCTTGTTGCTTGGCGAAA 3’. Left flank: 5' ccagggtataaaacacaaaaaaactacaaa 3'; Right flank: 5' ATGTCGGATCCAGTCGCGGATTTTTTGGCT 3’ (1 silent mutation); sgRNA: ctacaaaATGTCGGATCCAG; Cas9/sgRNA plasmid: pGLOW128; mScarlet^SEC^3xMyc plasmid: pGLOW156; SEC insertion allele strain: GLW92. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Cindy Hu (Glow Worms '22) Received: 01/10/23 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GLW95 Species: Caenorhabditis elegans Genotype: F13E6.1(utx75[F13E6.1::mNG::3xFlag]) X. Description: C-terminal tag of F13E6.1 via CRISPR/Cas9 knock-in of mNeonGreen at F13E6.1 locus. Insertion verified by PCR and fluorescence. Genotyping primers: fwd – 5’ AAATCGTGCTCTCCCAAGCA 3’; rev – 5’ CTTGTCACCTGACGGGATGT 3’. Left flank: 5' CCAGTTGCGGAGGAGGCGAAGCCAATCTCT 3' (1 silent mutation); Right flank: 5' TAAattcattcatttcacataccaatatgt 3'; sgRNA: atgaatTTAAGAGATTGGCT; Cas9/sgRNA plasmid: pGLOW26; mNG^SEC^3xFlag plasmid: pGLOW104; SEC insertion allele strain: GLW94. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Gonzalo Botello-Lins (Glow Worms '20) Received: 01/10/23 The University of Texas at Austin ----------------------------------------------------------------------------- Strain: GM209 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Seville, Spain by Patricia Rios Castano and Manuel Jesus Munoz Ruiz. Mutagen: Outcrossed: x Made by: Castano & Munoz Ruiz Received: 08/19/11 Universidad Pablo de Olavide, Sevilla, Spain ----------------------------------------------------------------------------- Strain: GMC101 Species: Caenorhabditis elegans Genotype: dvIs100. Description: dvIs100 [unc-54p::A-beta-1-42::unc-54 3'-UTR + mtl-2p::GFP]. mtl-2p::GFP produces constitutive expression of GFP in intestinal cells. unc-54p::A-beta-1-42 expresses full-length human A-beta-1-42 peptide in bodywall muscle cells that aggregates in vivo. Shifting L4 or young adult animals from 20C to 25C causes paralysis. Reference: McColl G, et al. Mol Neurodegener. 2012 Nov 21;7:57. Mutagen: Outcrossed: x4 Made by: Received: 01/17/13 The Florey Institute of Neuroscience ----------------------------------------------------------------------------- Strain: GN112 Species: Caenorhabditis elegans Genotype: pgIs2. Description: pgIs2 [gcy-8p::TU#813 + gcy-8p::TU#814 + unc-122p::GFP + gcy-8p::mCherry + gcy-8p::GFP + ttx-3p::GFP]. TU#813 and TU#814 are split caspase vectors (Chalfie Lab) subcloned downstream of the gcy-8 promoter. Expression of GFP in coelomocytes and AIY neuron, and mCherry in AFD neuron when caspase activity is lost. Reference: Glauser DA, et al. Genetics. 2011 May;188(1):91-103. Mutagen: Outcrossed: x1 Made by: B MacInnis/A Hellman Received: 12/01/11 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: GN231 Species: Caenorhabditis elegans Genotype: pgIR6 (X, CB4856>N2) X. Description: pgIR6 (X, CB4856>N2). CB4856 Chromosome X in N2 background. Mutagen: Outcrossed: x Made by: Will Chen Received: 06/07/13 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: GN517 Species: Caenorhabditis elegans Genotype: pgEx116. Description: pgEx116 [unc-70::TSmod + myo3p::mCherry]. Pick animals with red fluorescence in body wall muscle to maintain array. The tension sensor module (TSMod) was inserted into the coding sequence of unc-70. TSmod consists of a donor (mTFP) and acceptor (Venus) fluorophore separated by a flexible linker made of 40 residues from the spider-silk flagelliform, which acts as an entropic nanospring suitable for estimating biologically relevant forces. Reference: Krieg M, et al. Nat Cell Biol. 2014 Mar;16(3):224-33. Mutagen: Outcrossed: x Made by: Michael Krieg Received: 01/20/16 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: GN600 Species: Caenorhabditis elegans Genotype: pgIs22 IV; oxIs95. Description: pgIs22 [unc-70::N-TSmod]. oxIs95 [pdi-2p::unc-70 + myo-2p::GFP]. The tension sensor module control (N-TSMod) was inserted at the N-terminus of unc-70. N-TSmod consists of a donor (mTFP) and acceptor (Venus) fluorophore separated by a flexible linker made of 40 residues from the spider-silk flagelliform, but is placed at the N-terminus of UNC-70 where it is not sensitive to force. pgIs22 was a spontaneous insertion of pgEx157. Reference: Kelley M, et al. Elife. 2015 Mar 23;4. Mutagen: Spontaneous Outcrossed: x1 Made by: Michael Krieg Received: 01/20/16 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: GN67 Species: Caenorhabditis elegans Genotype: pgIR2 (II, CB4856>N2) II. Description: pgIR2 (II, CB4856>N2). CB4856 Chromosome II in N2 background. Mutagen: Outcrossed: x Made by: Will Chen Received: 06/07/13 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: GN675 Species: C. elegans Genotype: tba-1(pg77[tba-1::TagRFP-T + loxP]) I; uIs31 III. Description: uIs31 [mec-17p::GFP] III. pg77[tba-1::TagRFP-T + loxP] is a CRISPR-mediated c-external tag in the endogenous tba-1 locus. Reference: Lockhead D. et al Mol Biol Cell. 2016 Nov 15; 27(23): 3717–3728. Mutagen: Outcrossed: x0 Made by: Dean Lockhead Received: 03/13/19 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: GN68 Species: Caenorhabditis elegans Genotype: pgIR3 (III, CB4856>N2) III. Description: pgIR3 (III, CB4856>N2). CB4856 Chromosome III in N2 background. Mutagen: Outcrossed: x Made by: Will Chen Received: 06/07/13 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: GN683 Species: C. elegans Genotype: tbb-1(pg79[tbb-1::TagRFP-T + loxP]) uIs31 III. Description: uIs31 [mec-17p::GFP] III. pg79[TBB-1::TagRFP-T + loxP] is a CRISPR-mediated c-external tag in the endogenous tba-1 locus. Reference: Lockhead D. et al Mol Biol Cell. 2016 Nov 15; 27(23): 3717–3728. Mutagen: Outcrossed: x0 Made by: Dean Lockhead Received: 03/13/19 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: GN688 Species: C. elegans Genotype: tba-2(pg81[tba-2::tagRFP-T + loxP]) I; uIs31 III Description: uIs31 [mec-17p::GFP] III. pg81[TBA-2::tagRFP-T + loxP] is a CRISPR-mediated c-external tag in the endogenous tba-2 locus. Reference: Lockhead D. et al Mol Biol Cell. 2016 Nov 15; 27(23): 3717–3728. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Dean Lockhead Received: 03/13/19 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: GN69 Species: Caenorhabditis elegans Genotype: pgIR4 (IV, CB4856>N2) IV. Description: pgIR4 (IV, CB4856>N2). CB4856 Chromosome IV in N2 background. Mutagen: Outcrossed: x Made by: Will Chen Received: 06/07/13 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: GN70 Species: Caenorhabditis elegans Genotype: pgIR5 (V, CB4856>N2) V. Description: pgIR5 (V, CB4856>N2). CB4856 Chromosome V in N2 background. Mutagen: Outcrossed: x Made by: Will Chen Received: 06/07/13 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: GOU1861 Species: Caenorhabditis elegans Genotype: wsp-1a(cas723[gfp::wsp-1a] IV; arx-2(cas725[arx-2::TagRFP]) V. Description: Constructed by crossing individual fluorescence knock-in worms. TagRFP inserted into the endogenous arx-2 gene at its C-terminus and GFP inserted into the endogenous wsp-1a gene at its N-terminus by Cas9-triggered homologous recombination. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Zhiwen Zhu Received: 08/13/18 Tsinghua University, Beijing, China ----------------------------------------------------------------------------- Strain: GOU2042 Species: Caenorhabditis elegans Genotype: eff-1(cas618 [eff-1::gfp]) II. Description: GFP inserted into the endogenous eff-1 gene at its C-terminus by Cas9-triggered homologous recombination. Green fluorescence enriched in V.a cell 20min before cell-cell fusion in larvae. Very weak fluorescence in hyp7 cell. Reference: Yang Y, et al. Dev Cell. 2017 Apr 10;41(1):107-120. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x3 Made by: Yihong Yang Received: 06/26/17 Tsinghua University, Beijing, China ----------------------------------------------------------------------------- Strain: GOU2043 Species: Caenorhabditis elegans Genotype: vab-10a(cas602[vab-10a::gfp]) I. Description: GFP inserted into the endogenous vab-10a gene at its C-terminus by Cas9-triggered homologous recombination. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x3 Made by: Yihong Yang Received: 06/26/17 Tsinghua University, Beijing, China ----------------------------------------------------------------------------- Strain: GOU2044 Species: Caenorhabditis elegans Genotype: vab-10a(cas627[vab-10a::TagBFP]) I. Description: vab-10a(cas627 [vab-10a::TagBFP]) I. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x3 Made by: Yihong Yang Received: 06/26/17 Tsinghua University, Beijing, China ----------------------------------------------------------------------------- Strain: GOU2162 Species: Caenorhabditis elegans Genotype: che-3(cas443[gfp::che-3]) I; xbx-1(cas502[xbx-1::tagRFP]) V. Description: Constructed by crossing individual fluorescence knock-in worms. GFP inserted into the endogenous che-3 locus at the N-terminus and tagRFP::3xFlag inserted into the endogenous xbx-1 locus at the C-terminus by Cas9-triggered homologous recombination. Fluorescence enriched in most if not all sensory cilia. Very weak fluorescence in the cell bodies of ciliated neurons. Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Peishan Yi Received: 05/30/17 Tsinghua University, Beijing, China ----------------------------------------------------------------------------- Strain: GOU2187 Species: Caenorhabditis elegans Genotype: klp-20(cas447[klp-20::gfp]) III; xbx-1(cas502[xbx-1::tagRFP]) V; ift-81(cas498[ift-81::tagBFP]) X. Description: Constructed by crossing individual fluorescence knock-in worms. GFP inserted into the endogenous klp-20 gene at its C-terminus, tagRFP::3xFlag inserted into the endogenous xbx-1 gene at its C-terminus and tagBFP inserted into the endogenous ift-81 gene at its C-terminus by Cas9-triggered homologous recombination. GFP enriched at the proximal region (middle segment) of amphid or phasmid sensory cilia while red and blue fluorescence enriched along the whole cilia. Very weak fluorescence in the cell bodies of ciliated neurons. Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Peishan Yi Received: 07/18/17 Tsinghua University, Beijing, China ----------------------------------------------------------------------------- Strain: GOU2362 Species: Caenorhabditis elegans Genotype: ift-74(cas499[ift-74::gfp]) II. Description: GFP inserted into the endogenous ift-74 locus at the C-terminus by Cas9-triggered homologous recombination. Green fluorescence enriched in most, if not, all sensory cilia. Very weak fluorescence in the cell bodies of ciliated neurons. Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Peishan Yi Received: 05/30/17 Tsinghua University, Beijing, China ----------------------------------------------------------------------------- Strain: GOU2364 Species: Caenorhabditis elegans Genotype: che-3(cas528[gfp::che-3(R1384C)]) I. Description: GFP inserted into the endogenous che-3 gene at its N-terminus and R1384C mutation introduced by Cas9-triggered homologous recombination. Lipophilic dye filling is superficially wild-type and the amphid and phasmid sensory cilia are mildly shortened without evident IFT particle accumulation. Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Peishan Yi Received: 07/18/17 Tsinghua University, Beijing, China ----------------------------------------------------------------------------- Strain: GOU2365 Species: Caenorhabditis elegans Genotype: che-3(cas512[gfp::che-3(R1693C)]) I. Description: GFP inserted into the endogenous che-3 gene at its N-terminus and R1693C mutation introduced by Cas9-triggered homologous recombination. Lipophilic dye filling is 100% defective and the amphid and phasmid sensory cilia are severely shortened without evident IFT particle accumulation. Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Peishan Yi Received: 05/30/17 Tsinghua University, Beijing, China ----------------------------------------------------------------------------- Strain: GOU2366 Species: Caenorhabditis elegans Genotype: che-3(cas511[gfp::che-3(K2935Q)]) I. Description: GFP inserted into the endogenous che-3 locus at its N-terminus and K2935Q mutation introduced by Cas9-triggered homologous recombination. Lipophilic dye filling is 100% defective and the amphid and phasmid sensory cilia are severely shortened with strong IFT particle accumulation. Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Peishan Yi Received: 05/30/17 Tsinghua University, Beijing, China ----------------------------------------------------------------------------- Strain: GOU2936 Species: Caenorhabditis elegans Genotype: spc-1(cas815[spc-1::GFP]) X. Description: Endogenous spc-1 locus tagged with GFP at C-terminus. Reference: Jia R, et al. (2019). Spectrin-based Membrane Skeleton Supports Ciliogenesis. PLoS Biology. Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Ru Jia Received: 11/04/19 Tsinghua University, Beijing, China ----------------------------------------------------------------------------- Strain: GOU3103 Species: Caenorhabditis elegans Genotype: unc-70(cas962[GFP::unc-70]) V. Description: GFP inserted at the N-terminus of the endogenous unc-70 locus. Reference: Jia R, et al. (2019). Spectrin-based Membrane Skeleton Supports Ciliogenesis. PLoS Biology. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x3 Made by: Ru Jia Received: 11/04/19 Tsinghua University, Beijing, China ----------------------------------------------------------------------------- Strain: GOU3237 Species: Caenorhabditis elegans Genotype: unc-70(cas983) V. Description: unc-70(cas983) removes nine amino acids (H590-L598) in the SCA5-associated deletion in β-spectrin by Cas9-triggered homologous recombination. Reference: Jia R, et al. (2019). Spectrin-based Membrane Skeleton Supports Ciliogenesis. PLoS Biology. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x3 Made by: Ru Jia Received: 11/04/19 Tsinghua University, Beijing, China ----------------------------------------------------------------------------- Strain: GOU3238 Species: Caenorhabditis elegans Genotype: spc-1(cas971[spc-1(L268P)]) X. Description: L268P mutation introduced in α-spectrin by Cas9-triggered homologous recombination. Reference: Jia R, et al. (2019). Spectrin-based Membrane Skeleton Supports Ciliogenesis. PLoS Biology. Mutagen: Outcrossed: x3 Made by: Ru Jia Received: 11/04/19 Tsinghua University, Beijing, China ----------------------------------------------------------------------------- Strain: GOU3599 Species: Caenorhabditis elegans Genotype: spc-1(cas1049[spc-1(L268P)::GFP]) X. Description: L268P mutation introduced in GFP-tagged α-spectrin by Cas9-triggered homologous recombination. Reference: Jia R, et al. (2019). Spectrin-based Membrane Skeleton Supports Ciliogenesis. PLoS Biology. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x3 Made by: Ru Jia Received: 11/04/19 Tsinghua University, Beijing, China ----------------------------------------------------------------------------- Strain: GOU3601 Species: Caenorhabditis elegans Genotype: unc-70(cas1050[unc-70(delta H590-L598)::GFP]) V. Description: unc-70(cas1050) removes nine amino acids (H590-L598) in the SCA5-associated deletion in β-spectrin and inserts GFP at N-terminus of the endogenous unc-70 locus. Reference: Jia R, et al. (2019). Spectrin-based Membrane Skeleton Supports Ciliogenesis. PLoS Biology. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x3 Made by: Ru Jia Received: 11/04/19 Tsinghua University, Beijing, China ----------------------------------------------------------------------------- Strain: GR1028 Species: Caenorhabditis elegans Genotype: mnDp33 (X;IV)/+ IV; let-43(mg49) lon-2(e678) X. Description: Animals heterozygous for mnDp33 are Lon and segregate Lon and larval lethals. (Animals which have lost mnDp33 are larval lethal. Animals which are homozygous for mnDp33 are also larval lethal (L1-L2).) Mutagen: EMS Outcrossed: x7 Made by: David Greenstein Received: 04/05/93 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1029 Species: Caenorhabditis elegans Genotype: let-44(mg41) lon-2(e678) X; mnDp31 (X;f). Description: Animals with mnDp31 are WT and segregate WT and dead embryos. (Animals which have lost mnDp31 arrest as embryos.) Mutagen: EMS Outcrossed: x7 Made by: David Greenstein Received: 04/05/93 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1032 Species: Caenorhabditis elegans Genotype: age-1(mg44)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT and DpyUnc. age-1(mg44) homozygotes from heterozygous mothers are WT and segregate only dauers at all temperatures. mg44 pka daf-23(mg44). Mutagen: EMS Outcrossed: x6 Made by: S. Gottlieb Received: 08/15/94 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1034 Species: Caenorhabditis elegans Genotype: ceh-18(mg57) X. Description: Mutation resulted from the imprecise loss of pk37::Tc1. Incompletely penetrant sterile and lethal. Defects in oocyte cell cycle arrest, gonad migration, and hypodermal differentiation. See also WBPaper00002965. Mutagen: Tc1 Outcrossed: x7 Made by: David Greenstein Received: 04/18/94 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1168 Species: Caenorhabditis elegans Genotype: age-1(mg44)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: age-1(mg44) homozygotes throw all dauers at all temperatures (maternal effect dauer constitutive); can be rescued zygotically. age-1(mg44) homozygous animals that are maternally rescued for dauer formation are long-lived. mg44 is a Trp405 Amber mutation. Heterozygotes are WT and segregate WT (1/3 of which throw only dauers) and DpyUncs. Mutagen: EMS Outcrossed: x6 Made by: S Gottlieb/A Sluder Received: 05/19/98 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1307 Species: Caenorhabditis elegans Genotype: daf-16(mgDf50) I. Description: Deficiency completely eliminates daf-16 coding region. Makes partial dauers on pheromone. Mutagen: Gamma Rays Outcrossed: x3 Made by: S. Gottlieb Received: 12/18/97 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1308 Species: Caenorhabditis elegans Genotype: daf-16(mg54) I; daf-2(e1370) III. Description: mg54 almost completely suppresses the daf-c phenotype of daf-2. 0.4% dauers. Mutagen: EMS Outcrossed: x3 Made by: H. Tissenbaum Received: 12/18/97 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1309 Species: Caenorhabditis elegans Genotype: daf-16(mgDf47) I; daf-2(e1370) III. Description: mgDf47 completely suppresses daf-c phenotype of daf-2. mgDf47 deletes approximately 8kb of the daf-16 gene beginning after exon 4. Mutagen: Gamma Rays Outcrossed: x4 Made by: S. Gottlieb Received: 12/18/97 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1310 Species: Caenorhabditis elegans Genotype: akt-1(mg144) V. Description: No visible phenotype. Dominant suppressor of daf-c phenotype of age-1. Mutagen: EMS Outcrossed: x3 Made by: S. Paradis Received: 10/07/98 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1311 Species: Caenorhabditis elegans Genotype: daf-3(mgDf90) X. Description: mgDf90 completely eliminates the daf-3 coding region. Mutagen: mut-6 background Outcrossed: x3 Made by: Garth Patterson Received: 12/18/97 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1318 Species: Caenorhabditis elegans Genotype: pdk-1(mg142) X. Description: No visible phenotype. Dominant suppressor of daf-c phenotype of age-1. Ala303Val substitution. Mutagen: EMS Outcrossed: x4 Made by: S. Paradis Received: 05/11/99 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1321 Species: Caenorhabditis elegans Genotype: tph-1(mg280) cam-1(vs166) II. Description: Slow pumping. Egg laying defective. Low frequency of dauer formation. Residual serotonin immunoreactivity, rare and very faint (<1% of NSMs) in most stages, but nearly 100% of CP neurons in adult males show faint to moderate serotonin immunoreactivity. Males mate quite well (E. Hare and C. Loer). Some phenotypic defects originally attributed to mg280 in this strain are likely due to vs166. vs166 is a large deletion (approx. 9.8kb) in the cam-1 gene; flanking sequences are: 5'-gctactggtaaataaggtaa-3' and 5'-atgcttttaaagtttatatt-3' (Edith Myers, personal commnication). See MT15434 for a strain carrying mg280 without the cam-1 mutation. Mutagen: Diepoxyoctane Outcrossed: x4 Made by: Ji Ying Sze Received: 09/21/00 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: GR1322 Species: Caenorhabditis elegans Genotype: pdk-1(sa680) X; mgEx470. Description: mgEx470 [pdk-1(+) + ttx-3::GFP]. Pick GFP+ to maintain. 9.2-kb PCR product of genomic DNA from the pdk-1(+) genomic region containing 2.7 kb of 5' upstream regulatory sequence, 6.1 kb of coding sequencing containing introns and exons, and 0.4 kb of pdk-1 3' UTR. Reference: Paradis S, et al. Genes Dev. 1999 Jun 1;13(11):1438-52. Mutagen: Outcrossed: x Made by: Susan Paradis Received: 05/01/15 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1333 Species: C. elegans Genotype: yzIs71 V. Description: yzIs71 [tph-1p::GFP + rol-6(su1006)] V. Rollers. tph-1 transcriptional reporter containing 3.1kb of tph-1 5’ regulatory sequence through the beginning of exon 4. Reference: Lloret-Fernández et al. eLife 2018;7:e32785 DOI: 10.7554/eLife.32785. Sze JY, et al. Nature. 2000 Feb 3;403(6769):560-4. Mutagen: UV irradiation for integration Outcrossed: x4 Made by: unknown Received: 09/04/18 Instituto de Biomedicina de Valencia (IBV-CSIC), Valencia, Spain ----------------------------------------------------------------------------- Strain: GR1336 Species: Caenorhabditis elegans Genotype: daf-2(e1370) III; njEx32. Description: njEx32 [ges-1p::daf-2(+) + ges-1p::GFP + rol-6(su1006)]. Pick Rollers to maintain. Reference: Wolkow CA, et al. Science. 2000 Oct 6;290(5489):147-50. Mutagen: Outcrossed: x Made by: Cathy Wolkow Received: 05/01/15 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1337 Species: Caenorhabditis elegans Genotype: daf-2(e1370) III; njEx38. Description: njEx38 [unc-54p::daf-2(cDNA)::unc-54 3'UTR + unc-54p::GFP + rol-6(su1006)]. Pick Rollers to maintain. Reference: Wolkow CA, et al. Science. 2000 Oct 6;290(5489):147-50. Mutagen: Outcrossed: x Made by: Cathy Wolkow Received: 05/01/15 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1339 Species: Caenorhabditis elegans Genotype: daf-2(e1370) III; mgEx376. Description: mgEx376 [unc-14p::daf-2 + rol-6(su1006)]. Pick Rollers to maintain. Reference: Wolkow CA, et al. Science. 2000 Oct 6;290(5489):147-50. Mutagen: Outcrossed: x Made by: Cathy Wolkow Received: 05/01/15 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1340 Species: Caenorhabditis elegans Genotype: daf-2(e1370) III; mgEx373. Description: mgEx373 [unc-119p::daf-2(cDNA)::unc-54 3'UTR + rol-6(su1006)]. Pick Rollers to maintain. Reference: Wolkow CA, et al. Science. 2000 Oct 6;290(5489):147-50. Mutagen: Outcrossed: x Made by: Cathy Wolkow Received: 05/01/15 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1352 Species: Caenorhabditis elegans Genotype: daf-16(mgDf47) I; xrIs87. Description: xrIs87 [daf-16(alpha)::GFP::daf-16B + rol-6(su1006)]. Rollers. GFP expressed in many tissues. Partially rescued for daf-16 (daf-d). Mutagen: UV Outcrossed: x3 Made by: Raymond Lee Received: 10/21/05 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1366 Species: Caenorhabditis elegans Genotype: mgIs42. Description: mgIs42 [tph-1::GFP + rol-6(su1006)]. Rollers. Mutagen: Outcrossed: x4 Made by: Ji Ying Sze Received: 05/04/04 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1371 Species: Caenorhabditis elegans Genotype: lars-2(mg312) unc-29(e1072) I; nDp4 (I;V)/+. Description: lars-2(mg312) unc-29(e1072) homozygotes are Unc, slow growing, sterile, and have extended life span. Balanced worms are slightly Egl, otherwise WT. Mutagen: EMS Outcrossed: x Made by: Sylvia Lee Received: 07/28/03 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1373 Species: Caenorhabditis elegans Genotype: eri-1(mg366) IV. Description: Temperature sensitive: sterile at 25C. Maintain at 15C. Him. Eri. Due to a direct repeat the exact site and sequence of the 23 bp eri-1(mg366) insertion is unclear, however, the insertion lies in exon 6 of T07A9 between nucleotide positions 35215 and 35204 of cosmid T07A9 and includes 23 or these 32 nucleotides tttatcgaaaaaaaaacaggcactttatcgaa. Primers to follow eri-1mg366: GATAAAACTTCGGAACATATGGGGC and ACTGATGGGTAAGGAATCGAAGACG. These primers will give a 170 bp product in N2 and a 193 bp product in eri-1(mg366). Mutagen: EMS Outcrossed: x5 Made by: Scott Kennedy Received: 02/26/04 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1379 Species: Caenorhabditis elegans Genotype: lin-35(n745) I; eri-1(mg366) IV. Description: Temperature sensitive sterile at 25C. Maintain at 15C. Mutagen: Outcrossed: x Made by: Duo Wang Received: 10/13/05 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1395 Species: C. elegans Genotype: mgIs49 IV. Description: mgIs49 [mlt-10p::GFP::PEST + ttx-3::GFP] IV. GFP expression oscillates with molting cycle. Reference: Veli SM, et al. Mol Biol Cell. 2010 May 15;21(10):1648-61. PMID: 20335506. Mutagen: Outcrossed: x0 Made by: Alison R Frand Received: 11/03/20 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: GR1428 Species: Caenorhabditis elegans Genotype: mgIs45 I. Description: mgIs45 [mir-84(+) + tub-1::GFP] I. mir-84 over-expressing line. Reference: Hayes GD, Riedel CG, Ruvkun G. Genes Dev. 2011 Oct 1;25(19):2079-92. Mutagen: Outcrossed: x4 Made by: Gabe Hayes Received: 05/01/15 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1431 Species: Caenorhabditis elegans Genotype: mir-84(tm1304) X. Description: Enhances retarded differentiation of the hypodermis and exit from the molting cycle caused by mutations in let-7 or its paralogs. Reference: Hayes GD, Frand AR, Ruvkun G. Development. 2006 Dec;133(23):4631-41. Mutagen: UV/TMP Outcrossed: x8 Made by: Gabriel Hayes Received: 06/25/12 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1432 Species: Caenorhabditis elegans Genotype: let-7(mg279) X. Description: Weakly retarded differentiation of the hypodermis and exit from the molting cycle. Reference: Hayes GD, Frand AR, Ruvkun G. Development. 2006 Dec;133(23):4631-41. Mutagen: Outcrossed: x>2 Made by: Gabriel Hayes Received: 06/25/12 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1433 Species: Caenorhabditis elegans Genotype: let-7(mg279) mir-84(tm1304) X. Description: Retarded differentiation of the hypodermis and supernumerary molt that results in adult lethality. Reference: Hayes GD, Frand AR, Ruvkun G. Development. 2006 Dec;133(23):4631-41. Mutagen: Outcrossed: x>2 Made by: Gabriel Hayes Received: 06/25/12 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1452 Species: Caenorhabditis elegans Genotype: veIs13 V; let-7(mn112) unc-3(e151) X; mgEx725. Description: veIs13 [col-19::GFP + rol-6(su1006)] V. mgEx725 [lin-4::let-7 + ttx-3::RFP]. Pick RFP+ to maintain. mgEx725 rescues lethality of let-7(mn112). Precocious expression of col-19::GFP at the L4 stage. Reference: Hayes GD, Riedel CG, Ruvkun G. 2011. Genes Dev. 2011 Oct 1;25(19):2079-92. Mutagen: Outcrossed: x Made by: Gabriel Hayes Received: 06/25/12 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1455 Species: Caenorhabditis elegans Genotype: mgIs40. Description: mgIs40 [daf-28p::GFP]. Mutagen: UV Outcrossed: x5 Made by: Weiqing Li Received: 11/27/06 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: GR1583 Species: Caenorhabditis elegans Genotype: somi-1(mg415) V. Description: Adults slightly Dpy. Suppresses defects caused by over-expression of mir-84. Enhances retarded heterchronic phenotypes caused by a weak allele of let-7 or loss of mir-84. Reference: Hayes GD, Riedel CG, Ruvkun G. 2011. Genes Dev. 2011 Oct 1;25(19):2079-92. Mutagen: EMS Outcrossed: x4 Made by: Gabriel Hayes Received: 06/25/12 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1586 Species: Caenorhabditis elegans Genotype: somi-1(tm562) V. Description: Adults slightly Dpy. Suppresses defects caused by over-expression of mir-84. Enhances retarded heterchronic phenotypes caused by a weak allele of let-7 or loss of mir-84. Reference: Hayes GD, Riedel CG, Ruvkun G. 2011. Genes Dev. 2011 Oct 1;25(19):2079-92. Mutagen: UV/TMP Outcrossed: x7 Made by: Gabriel Hayes Received: 06/25/12 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1589 Species: Caenorhabditis elegans Genotype: mgIs45 I; somi-1(mg431) wIs54 V. Description: mgIs45 [mir-84(over-expressing) + tub-1::GFP] I. wIs54 [scm::GFP] V. Maintain by picking animals with good expression of tub-1::GFP in amphid neurons to maintain. somi-1 mutation suppresses precocious development of the vulva and hypodermal cells caused by over-expression of mir-84. Reference: Hayes GD, Riedel CG, Ruvkun G. 2011. Genes Dev. 2011 Oct 1;25(19):2079-92. Mutagen: Outcrossed: x Made by: Gabriel Hayes Received: 06/25/12 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1672 Species: Caenorhabditis elegans Genotype: mgEx340. Description: mgEx340 [akt-1::GFP::unc-54 3'UTR + rol-6(su1006)]. Pick Rollers to maintain. AKT-1::GFP translational fusion containing 6.7 kb akt-1 genomic DNA including 3.2 kb of 5' upstream regulatory region and 3.5 kb of coding region (including exons and introns) fused in-frame to GFP with unc-54 3' UTR. Reference: Paradis S, Ruvkun G. Genes Dev. 1998 Aug 15;12(16):2488-98. Mutagen: Outcrossed: x Made by: Susan Paradis Received: 05/01/15 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1673 Species: Caenorhabditis elegans Genotype: mgEx341. Description: mgEx341 [akt-2::GFP::unc-54 3'UTR + rol-6(su1006)]. Pick Rollers to maintain. AKT-2::GFP translational fusion containing 5.2 kb akt-1 genomic DNA including 2.1 kb of 5' upstream regulatory region and 3.1 kb of coding region (including exons and introns) fused in-frame to GFP with unc-54 3' UTR. Reference: Paradis S, Ruvkun G. Genes Dev. 1998 Aug 15;12(16):2488-98. Mutagen: Outcrossed: x Made by: Susan Paradis Received: 05/01/15 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1674 Species: Caenorhabditis elegans Genotype: mgEx481. Description: mgEx481 [pdk-1::GFP::unc-54 3'UTR + rol-6(su1006)]. Pick Rollers to maintain. PDK-1::GFP translational fusion containing 9 kb akt-1 genomic DNA including 2.9 kb of 5' upstream regulatory region and 6.1 kb of coding region (including exons and introns) fused in-frame to GFP with unc-54 3' UTR. Reference: Paradis S, et al. Genes Dev. 1999 Jun 1;13(11):1438-52. Mutagen: Outcrossed: x Made by: Susan Paradis Received: 05/01/15 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1719 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; mgSi3 IV. Description: mgSi3 [(pCMP2)ubl-1p::GFP::ubl-1-3'UTR + Cbr-unc-119(+)] IV. Strong ubiquitous GFP expression. Can be used as a control for a strain containing an endogenous siRNA sensor (GR1720). Reference: Montgomery TA, et al. PLoS Genet. 2012;8(4):e1002616. Mutagen: Outcrossed: x0 Made by: Tai Montgomery Received: 11/04/13 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1720 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; mgSi4 IV. Description: mgSi4 [(pCMP2)ubl-1p::GFP::siR-1-sensor-ubl-1-3'UTR + Cbr-unc-119(+)] IV. Very weak ubiquitous GFP expression. The 22G siR-1 sensor transgene is more sensitive to gene inactivations that affect 22G siR-1 levels when grown at 25C compared to 20C. Reference: Montgomery TA, et al. PLoS Genet. 2012;8(4):e1002616. Mutagen: Outcrossed: x0 Made by: Tai Montgomery Received: 11/04/13 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1747 Species: Caenorhabditis elegans Genotype: mut-15(tm1358) V. Description: Him. Mutator. RNAi-defective. Temperature-sensitive sterile at 25C. Reference: Phillips CM, et al. Genes Dev. 2012 Jul 1;26(13):1433-44. Mutagen: UV/TMP Outcrossed: x4 Made by: S Mitani/C Phillips Received: 12/17/12 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1748 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; mgSi2 IV. Description: mgSi2 [mut-16p::mut-16::GFP::mut-16 3'UTR + unc-119(+)] IV. MosSCI integrant of mut-16::GFP rescues pk710. Reference: Phillips CM, et al. Genes Dev. 2012 Jul 1;26(13):1433-44. Mutagen: Outcrossed: x Made by: Carolyn Phillips Received: 05/01/15 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1808 Species: Caenorhabditis elegans Genotype: rde-10(mg458) I. Description: RNAi-defective. Reference: Zhang C, et al. Curr Biol. 2012 May 22;22(10):881-90. Mutagen: EMS Outcrossed: x3 Made by: Chi Zhang Received: 12/17/12 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1814 Species: Caenorhabditis elegans Genotype: eri-6(mg379) I. Description: Reference: Fischer SE, et al. Nature. 2008 Sep 25;455(7212):491-6. Mutagen: Outcrossed: x Made by: Ruvkun Lab Received: 07/29/13 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1823 Species: Caenorhabditis elegans Genotype: mut-16(mg461) I. Description: RNAi-deficient in soma. Reference: Zhang C, et al. Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1201-8. Mutagen: Outcrossed: x Made by: Chi Zhang Received: 12/17/12 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1895 Species: Caenorhabditis elegans Genotype: daf-2(e1370) III; mgIs67. Description: mgIs67 [daf-16p::daf-16::GFP + rol-6(su1006)]. Temperature-sensitive. Daf-c. Maintain at 15C. Dauer formation at 25C. Slow growing. Dauer-like at 20C. DAF-16::GFP is fully nuclear at 20C. Reference: Riedel CG, et al. Nat Cell Biol. 2013 May;15(5):491-501. Mutagen: Outcrossed: x Made by: Christian Riedel Received: 05/01/15 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR1971 Species: Caenorhabditis elegans Genotype: mgEx779. Description: mgEx779 [lipl-4p::K04A8.5p::lipl-4::SL2::GFP + myo-2p::mCherry]. Polycistronic translational fusion; GFP and LIPL-4 expressed separately. Pick animals with red pharynx to maintain. Reference: Wang et al. Science. 2008 Nov 7;322(5903):957-60. Mutagen: Outcrossed: x Made by: Meng Wang Received: 11/26/12 Buck Institute for Research on Aging ----------------------------------------------------------------------------- Strain: GR1973 Species: Caenorhabditis elegans Genotype: mgIs63. Description: mgIs63 [sdf-9p::RFP]. RFP expressed in the XXX cells. Reference: Hu PJ, Xu J, Ruvkun G. PLoS Genet. 2006 Jul;2(7):e99. Mutagen: UV Outcrossed: x5 Made by: Patrick Hu Received: 08/17/12 Vanderbilt University Medical Center, Nashville, TN ----------------------------------------------------------------------------- Strain: GR2062 Species: Caenorhabditis elegans Genotype: eri-7(mg369) I. Description: Loss-of-function allele; stronger than eri-7(mg411). Reference: Fischer SE, et al. Nature. 2008 Sep 25;455(7212):491-6. Mutagen: Outcrossed: x Made by: Ruvkun Lab Received: 07/29/13 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR2063 Species: Caenorhabditis elegans Genotype: hsd-1(mg433) I. Description: Weak Hid. Eak. Mutagen: EMS Outcrossed: x1 Made by: Weiqing Li Received: 07/23/13 Vanderbilt University Medical Center, Nashville, TN ----------------------------------------------------------------------------- Strain: GR2107 Species: Caenorhabditis elegans Genotype: daf-2(e1370) III; mgEx371. Description: mgEx371 [dpy-30p::daf-2(cDNA)::unc-54 3'UTR + rol-6(su1006)]. Pick Rollers to maintain. Reference: Wolkow CA, et al. Science. 2000 Oct 6;290(5489):147-50. Mutagen: Outcrossed: x Made by: Cathy Wolkow Received: 05/01/15 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR2116 Species: Caenorhabditis elegans Genotype: mgEx579. Description: mgEx579 [ins-18p::GFP + rol-6(su1006)]. Pick Rollers to maintain. ins-18::GFP promoter fusion containing 4.9 kb upstream regulatory sequence and 2.1 kb of coding region (including exons and introns) driving GFP expression with 0.8 kb downstream sequence. Reference: Pierce SB, et al. Genes Dev. 2001 Mar 15;15(6):672-86. Mutagen: Outcrossed: x Made by: Sarah Pierce Received: 05/01/15 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR2117 Species: Caenorhabditis elegans Genotype: daf-2(e1365) III; mgIs35. Description: mgIs35 [ins-1(genomic) + mec-7p::GFP]. Temperature-sensitive. Maintain at 15C. Daf-c. High penetrance of dauer formation at 20C. Integration of mgEx557. Reference: Pierce SB, et al. Genes Dev. 2001 Mar 15;15(6):672-86. Mutagen: Gamma radiation Outcrossed: x Made by: Sarah Pierce Received: 05/01/15 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR2118 Species: Caenorhabditis elegans Genotype: daf-9(e1406) dpy-7(sc27) X; mgEx663. Description: mgEx663 [dpy-7p::daf-9B(cDNA)::GFP + mec-7::GFP]. Pick GFP+ to maintain. GFP fusion containing 0.4 kb dpy-7 promoter and daf-9 isoform B cDNA. Reference: Mak HY, Ruvkun G. Development. 2004 Apr;131(8):1777-86. Mutagen: Outcrossed: x Made by: Ho Yi Mak Received: 05/01/15 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR2183 Species: Caenorhabditis elegans Genotype: mgIs72 II. Description: mgIs72 [rpt-3p::GFP + dpy-5(+)] II. Reporter of proteasome subunit expression can be used to assay skn-1a-dependent regulation of proteasome subunit genes. mgIs72 [rpt-3::gfp] integrated transgene was generated from sEx15003. Mutagen: EMS Outcrossed: x3 Made by: Nicholas Lehrbach Received: 06/01/17 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR2198 Species: Caenorhabditis elegans Genotype: mgTi1 I; unc-119(ed3) III. Description: mgTi1 [rpl-28p::skn-1a::GFP::tbb-2 3'UTR + unc-119(+)] I. Expresses SKN-1A with C-terminal GFP tag. Reference: Lehrbach NL & Ruvkun G. Elife. 2016 Aug 16;5. pii: e17721 Mutagen: minimos insertion Outcrossed: x0 Made by: Nicholas Lehrbach Received: 12/18/17 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR2203 Species: Caenorhabditis elegans Genotype: ddi-1(mg572) IV. Description: Superficially wild-type.  Mutation in active site of ddi-1 protease. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Nicholas Lehrbach Received: 06/01/17 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR2214 Species: Caenorhabditis elegans Genotype: sel-1(mg547) V. Description: Superficially wild-type. Mutagen: EMS Outcrossed: x2 Made by: Nicholas Lehrbach Received: 06/01/17 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR2232 Species: Caenorhabditis elegans Genotype: sel-9(mg550) V. Description: Superficially wild-type. Mutagen: EMS Outcrossed: x2 Made by: Nicholas Lehrbach Received: 06/01/17 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR2245 Species: Caenorhabditis elegans Genotype: skn-1(mg570) IV. Description: Superficially wild-type Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Nicholas Lehrbach Received: 06/01/17 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR2247 Species: Caenorhabditis elegans Genotype: mdt-15(mg584) III. Description: Gain of function allele of mdt-15. Reference: Mao K, et al. Cell Metab. 2019 Feb 14. pii: S1550-4131(19)30022-1. Mutagen: Outcrossed: x0 Made by: Kai Mao Received: 03/21/19 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR2250 Species: Caenorhabditis elegans Genotype: mgIs73 V. Description: mgIs73 [cyp-14A4p::gfp::cyp-14A4 3’UTR + myo-2p::mCherry] V. Reference: Mao K, et al. Cell Metab. 2019 Feb 14. pii: S1550-4131(19)30022-1. Mutagen: Integrated by EMS Outcrossed: x13 Made by: Kai Mao Received: 03/21/19 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR2252 Species: C. elegans Genotype: hsp-6(mg585) V; mgIs73 V. Description: mgIs73 [cyp-14A4p::GFP::cyp-14A4 3’UTR + myo-2p::mCherry] V. Slow growth. Low brood size. Received as a replacement for GR2249. Reference: Mao K, et al. Cell Metab. 2019 Feb 14. pii: S1550-4131(19)30022-1. Mutagen: N/A Outcrossed: x0 Made by: Ruvkun Received: 04/04/22 University of Minnesota, Minneapolis MN, USA ----------------------------------------------------------------------------- Strain: GR2255 Species: Caenorhabditis elegans Genotype: moc-2(mg595) V. Description: Can be maintained on OP50. Requires dietary Moco for viability. Reference: Warnhoff K & Ruvkun G. Nat Chem Biol. 2019 Mar 25. doi: 10.1038/s41589-019-0249-y. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Kurt Warnhoff Received: 04/16/19 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR2256 Species: Caenorhabditis elegans Genotype: F49E2.1(mg589) X. Description: F49E2.1. Can be maintained on OP50. Requires dietary Moco for viability. Reference: (F49E2.1 referred to as moc-5) Warnhoff K & Ruvkun G. Nat Chem Biol. 2019 Mar 25. doi: 10.1038/s41589-019-0249-y. Mutagen: EMS Outcrossed: x6 Made by: Kurt Warnhoff Received: 04/16/19 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR2257 Species: Caenorhabditis elegans Genotype: cth-2(mg599) II. Description: Can be maintained on OP50. Suppresses Moco-deficient larval lethality. Reference: Warnhoff K & Ruvkun G. Nat Chem Biol. 2019 Mar 25. doi: 10.1038/s41589-019-0249-y. Mutagen: EMS Outcrossed: x4 Made by: Kurt Warnhoff Received: 04/16/19 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR2260 Species: Caenorhabditis elegans Genotype: cdo-1(mg622) X. Description: Can be maintained on OP50. Suppresses Moco-deficient larval lethality. Reference: Warnhoff K & Ruvkun G. Nat Chem Biol. 2019 Mar 25. doi: 10.1038/s41589-019-0249-y. Mutagen: EMS Outcrossed: x4 Made by: Kurt Warnhoff Received: 04/16/19 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR2263 Species: Caenorhabditis elegans Genotype: nhr-45(mg641) X. Description: Reference: Mao K, et al. Cell Metab. 2019 Feb 14. pii: S1550-4131(19)30022-1. Mutagen: Outcrossed: x0 Made by: Kai Mao Received: 03/21/19 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR2272 Species: Caenorhabditis elegans Genotype: rnp-2(mg582) IV. Description: CRISPR/Cas9 used to engineer a 6bp in-frame deletion in 5' coding region removing 2 amino acid residues that are conserved from yeast to human that might be critical for U1A binding to U1 snRNA Reference: Newman MA, et al. Genes Dev. 2018 May 1;32(9-10):670-681. PMID: 29739806 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Martin Newman Received: 08/29/18 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR3055 Species: Caenorhabditis elegans Genotype: suox-1(mg663)/tmC24 [F23D12.4(tmIs1240) unc-9(tm9719)] X Description: Larval lethal mutation balanced by tmC24 [F23D12.4(tmIs1240) unc-9(tm9719)]. Balancer marked with myo-2p::Venus. Maintain by picking non-Unc GFP+ animals. Heterozygotes are wild-type GFP+ and segregate wild-type GFP+ heterozygotes, non-GFP mg663 homozygotes (lethal), and Unc GFP+ (homozygous tmC24). Reference: Warnhoff K & Ruvkun G. Nat Chem Biol. 2019 Mar 25. doi: 10.1038/s41589-019-0249-y. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Kurt Warnhoff Received: 04/16/19 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR3090 Species: C elegans Genotype: mgIs77 V. Description: mgIs77 [rpl-28p::ub(G76V)::GFP + unc-119(+) + myo-2p::mCherry] V. Reference: Lehrbach NJ, et al. Cell. 2019 Apr 18;177(3):737-750.e15. Mutagen: Outcrossed: x0 Made by: Nicholas Lehrbach Received: 05/09/19 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GR3149 Species: C elegans Genotype: mgIs72 II; mgIs78 IV. Description: mgIs72 [rpt-3p::GFP + dpy-5(+)] II. mgIs78 [myo-3p::H2B::mCherry::SL2::pbs-5(T65A)] IV. Muscle-specific proteasome dysfunction. Reference: Lehrbach NJ & Ruvkun G. Elife. 2019 Apr 11;8. pii: e44425. doi: 10.7554/eLife.44425. Mutagen: Outcrossed: x0 Made by: Nicholas Lehrbach Received: 05/09/19 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: GRU101 Species: C.elegans Genotype: gnaIs1. Description: gnaIs1 [myo-2p::yfp]. Control strain for pan-neuronal amyloid beta1-42 expressing strain GRU102. WT phenotype with pharyngeal YFP expression. Reference: Fong S, et al. Sci Rep. 2016 Sep 22;6:33781. doi: 10.1038/srep33781. Mutagen: Outcrossed: x4 Made by: Jan Gruber Received: 01/03/18 Yale - National University of Singapore ----------------------------------------------------------------------------- Strain: GRU102 Species: C.elegans Genotype: gnaIs2. Description: gnaIs2 [myo-2p::YFP + unc-119p::Abeta1-42]. Pan-neuronal amyloid beta1-42 expression. Impaired neuromuscular and sensorimotor behavior. See GRU101 for wild-type control strain. Reference: Fong S, et al. Sci Rep. 2016 Sep 22;6:33781. doi: 10.1038/srep33781. Mutagen: Outcrossed: x4 Made by: Jan Gruber Received: 01/03/18 Yale - National University of Singapore ----------------------------------------------------------------------------- Strain: GS10037 Species: C. elegans Genotype: arSi159 [rps-27p::GFP(flexon)::H2B::unc-54 3'UTR + Cbr-unc-119(+)] II; unc-119(ed3) III. Description: arSi159 [rps-27p::GFP(flexon)::H2B::unc-54 3'UTR + Cbr-unc-119(+)] II. Inserted into ttTi5605 (II). The first intron of GFP was replaced with a Flexon containing two loxP sites. GFP::H2B is expressed at a high level in lineages where Cre is expressed with a second transgene. Flexon contains two loxP sites. Reference: Wittes J & Greenwald I. (2024). New Flexon-based reagents for tissue-specific Auxin-Inducible Degradation and for characterizing Cre and Flp drivers in C. elegans. microPublication Biology. 10.17912/micropub.biology.001315. Mutagen: MosSCI Outcrossed: x0 Made by: Julia Wittes Received: 09/19/24 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS107 Species: Caenorhabditis elegans Genotype: unc-32(e189) lin-12(n676n930) III; dpy-11(e224) sel-9(ar22) V. Description: DpyUnc. At 25C ar22 recessively suppresses the Egl defect of n676n930. At 15C ar22 dominantly suppresses the 2 AC-Egl phenotype of n676n930. Suppresses the vulval lineage defects and proximal mitosis defects of lin-12. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x>2 Made by: Meera Sundaram Received: 01/25/94 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS1214 Species: Caenorhabditis elegans Genotype: sel-12(ar171) unc-1(e538) X. Description: Egl. Unc. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x Made by: Received: 04/05/05 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS1369 Species: Caenorhabditis elegans Genotype: emb-5(hc61) III. Description: Temperature sensitive maternal effect lethal if shifted from 15C to 25C at the L4 and later stages; sterile if it gets past embryogenesis before being shifted. Maintain at 15C. MJ61 contains a lin-12 allele (ar170) which results in 2 anchor cells. GS1369 was derived by outcrossing MJ61 to remove ar170. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Added 10/2005: Zhuoyu Ni in Sylvia Lee's lab did not find the published missense mutation in this strain. It does have the ts sterile/lethal phenotype still. Mutagen: Outcrossed: x Made by: Jane Hubbard Received: 04/15/96 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS1692 Species: Caenorhabditis elegans Genotype: unc-4(e120) II; arDp2 (II;f). Description: Pick non-Uncs to maintain. arDp2 is derived from mnC1, hence carries dpy-10(e128) and possibly unc-52(e444). arDp2 is a free duplication but does not pass at a high frequency. arDp2 is not stable as a balancer. It is prone to recombination; pick non-Unc and check for segregation of unc-4 and WT in progeny. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Diane Levitan Received: 12/13/06 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS1826 Species: Caenorhabditis elegans Genotype: dpy-20(e1282) IV; arIs36. Description: arIs36 [(pJF43) hsp::ssGFP + dpy-20(+) + pBluescript SK+]. GFP is secreted into the pseudocoelom upon heat shock and is taken up by coelomocytes. Not known where arIs36 is attached. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Johnny Fares Received: 05/16/01 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: GS1829 Species: Caenorhabditis elegans Genotype: sel-2(n655) III. Description: Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x Made by: Received: 05/03/07 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS1894 Species: Caenorhabditis elegans Genotype: sel-12(ar131) X. Description: Egl. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x Made by: Received: 04/05/05 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS1912 Species: Caenorhabditis elegans Genotype: arIs37 I; dpy-20(e1282) IV. Description: arIs37 [myo-3p::ssGFP + dpy-20(+)] I. GFP is secreted from muscle cells. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: X-rays Outcrossed: x8 Made by: Johnny Fares Received: 05/16/01 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: GS195 Species: Caenorhabditis elegans Genotype: ncl-1(e1865) unc-36(e251) glp-1(q46) mua-3(ar62) III; qDp3 (III;f). Description: Animals with the duplication are WT and segregate WT and larval lethals. Larval muscle dettachment and arrest at about L1 stage in mua-3(ar62) homozygotes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Elizabeth Bucher Received: 05/17/99 University of Pennsylvania, Philadelphia ----------------------------------------------------------------------------- Strain: GS1953 Species: Caenorhabditis elegans Genotype: sel-2(ar219) III. Description: Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Hanna Fares Received: 05/03/07 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS234 Species: Caenorhabditis elegans Genotype: mup-4(ar60) ncl-1(e1865) unc-36(e251) glp-1(q46) III; qDp3 (III;f). Description: Animals with the duplication are WT and segregate WT and dead eggs. 3-fold muscle dettachment and arrest in 90% of mup-4(ar60) homozygotes; 10% arrest earlier. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Elizabeth Bucher Received: 05/17/99 University of Pennsylvania, Philadelphia ----------------------------------------------------------------------------- Strain: GS2381 Species: Caenorhabditis elegans Genotype: sel-5(ok149) III. Description: WT. Suppresses the egg-laying defect of lin-12(n302). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: UV/Psoralen Outcrossed: x2 Made by: Moulder/Knockout Con Received: 09/03/99 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS2447 Species: Caenorhabditis elegans Genotype: hop-1(ar179) I. Description: Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: C. Wen Received: 05/26/05 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS2477 Species: Caenorhabditis elegans Genotype: arIs37 I; cup-5(ar465) III; dpy-20(e1282) IV. Description: arIs37 [myo-3p::ssGFP + dpy-20(+)] I. cup-5 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x3 Made by: Hanna Fares Received: 03/25/05 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: GS2478 Species: Caenorhabditis elegans Genotype: arIs37 I; dpy-20(e1282) IV; cup-8(ar466) V. Description: arIs37 [myo-3p::ssGFP + dpy-20(+)] I. cup-8 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x3 Made by: Hanna Fares Received: 03/25/05 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: GS2479 Species: Caenorhabditis elegans Genotype: arIs37 I; dpy-20(e1282) IV; cup-9(ar467) V. Description: arIs37 [myo-3p::ssGFP + dpy-20(+)] I. cup-9 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x3 Made by: Hanna Fares Received: 03/25/05 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: GS2484 Species: Caenorhabditis elegans Genotype: arIs37 I; dpy-20(e1282) IV; cup-11(ar472) X. Description: arIs37 [myo-3p::ssGFP + dpy-20(+)] I. cup-11 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x3 Made by: Hanna Fares Received: 03/25/05 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: GS2495 Species: Caenorhabditis elegans Genotype: arIs37 I; dpy-20(e1282) IV; mtm-9(ar479) V. Description: arIs37 [myo-3p::ssGFP + dpy-20(+)] I. Coelomocyte endocytosis defect. GFP accumulates in body cavity. MTM-9=Y39H10A.3 mtm-9 pka cup-10. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x3 Made by: Johnny Fares Received: 04/27/04 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: GS2526 Species: Caenorhabditis elegans Genotype: arIs37 I; mca-3(ar492) dpy-20(e1282) IV. Description: arIs37 [myo-3p::ssGFP + dpy-20(+)] I. mca-3 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x3 Made by: Hanna Fares Received: 03/25/05 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: GS2527 Species: Caenorhabditis elegans Genotype: arIs37 I; mca-3(ar493) dpy-20(e1282) IV. Description: arIs37 [myo-3p::ssGFP + dpy-20(+)] I. mca-3 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x3 Made by: Hanna Fares Received: 03/25/05 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: GS2532 Species: Caenorhabditis elegans Genotype: arIs37 I; cup-3(ar498) II; dpy-20(e1282) IV. Description: arIs37 [myo-3p::ssGFP + dpy-20(+)] I. cup-3 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x3 Made by: Hanna Fares Received: 03/25/05 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: GS2555 Species: Caenorhabditis elegans Genotype: cup-2(ar506) arIs37 I; dpy-20(e1282) IV. Description: arIs37 [myo-3p::ssGFP + dpy-20(+)] I. cup-2 is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x3 Made by: Hanna Fares Received: 03/25/05 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: GS2643 Species: Caenorhabditis elegans Genotype: arIs37 I; cup-5(ar465) III; dpy-20(e1282) IV. Description: arIs37 [myo-3p::ssGFP + dpy-20(+)] I. cup-5(ar465) is defective in endocytosis by coelomocytes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x3 Made by: Johnny Fares Received: 05/16/01 University of Arizona, Tucson, AZ ----------------------------------------------------------------------------- Strain: GS2735 Species: Caenorhabditis elegans Genotype: spr-2(ar211) dpy-20(e1282) IV. Description: Dpy. Suppresses presenilin genes. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x Made by: Received: 11/26/01 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS2744 Species: Caenorhabditis elegans Genotype: spr-2(ar199) dpy-20(e1282) IV. Description: Dpy. Suppresses presenilin genes. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x Made by: Received: 11/26/01 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS3012 Species: Caenorhabditis elegans Genotype: spr-1(ar200) V. Description: ar200 does not display any apparent phenotype. It suppresses the Egl defect of both sel-12(ar171) and sel-12(ar131), and displays genetic interactions with various lin-12 and glp-1 alleles. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x7 Made by: S. Jarriault Received: 04/01/03 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS304 Species: Caenorhabditis elegans Genotype: evl-16(ar93)/unc-11(e47) dpy-5(e61) I. Description: Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/25/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS305 Species: Caenorhabditis elegans Genotype: evl-17(ar94)/dpy-5(e61) unc-13(e51) I. Description: Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/14/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS307 Species: Caenorhabditis elegans Genotype: dpy-5(e61) cye-1(ar95)/unc-13(e51) I. Description: Heterozygotes are WT and segregate WT, Uncs and Sterile Dpys which have an everted vulva. ar95 previously called evl-10(ar95). See also WBPaper00004382. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/14/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS311 Species: Caenorhabditis elegans Genotype: unc-36(e251) evl-14(ar97)/dpy-17(e164) unc-32(e189) III. Description: Heterozygotes are WT and segregate WT, DpyUncs and Sterile Unc-36 which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 07/31/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS314 Species: Caenorhabditis elegans Genotype: evl-3(ar100)/dpy-10(e128) unc-4(e120) II. Description: Heterozygotes are WT and segregate WT, Steriles with and Everted Vul and DpyUncs. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/11/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS316 Species: Caenorhabditis elegans Genotype: unc-36(e251) evl-14(ar96)/dpy-17(e164) unc-32(e189) III. Description: Heterozygotes are WT and segregate WT, DpyUncs and Sterile Unc-36 which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 07/31/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS3165 Species: Caenorhabditis elegans Genotype: spr-1(ar205) V. Description: ar205 does not display any apparent phenotype. It suppresses the Egl defect of both sel-12(ar171) and sel-12(ar131). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x7 Made by: S. Jarriault Received: 04/01/03 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS318 Species: Caenorhabditis elegans Genotype: evl-3(ar99)/dpy-10(e128) unc-4(e120) II. Description: Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/05/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS320 Species: Caenorhabditis elegans Genotype: unc-36(e251) evl-8(ar102)/dpy-17(e164) unc-32(e189) III. Description: Heterozygotes are WT and segregate WT, DpyUncs and Unc Steriles which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/25/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS326 Species: Caenorhabditis elegans Genotype: evl-20(ar103)/dpy-10(e128) unc-4(e120) II. Description: Heterozygotes are WT and segregate WT, Steriles with and Everted Vul and DpyUncs. See also WBPaper00005256. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/11/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS327 Species: Caenorhabditis elegans Genotype: apc-1(ar104)/dpy-10(e128) unc-4(e120) II. Description: Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted vulva. Segregates males, so may have a him- mutation? ar104 previously called evl-22 and mat-2. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/04/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS328 Species: Caenorhabditis elegans Genotype: unc-36(e251) evl-19(ar98)/dpy-17(e164) unc-32(e189) III. Description: Heterozygotes are WT and segregate WT, DpyUncs and Sterile Unc-36 which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 07/31/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS357 Species: Caenorhabditis elegans Genotype: unc-42(e270) arDf1 V/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Uncs and dead eggs. nT1 carries a dominant Unc mutation and a recessive lethal mutation. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Gamma Rays Outcrossed: x3 Made by: Simon Tuck Received: 03/05/93 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS3582 Species: Caenorhabditis elegans Genotype: unc-4(e120) II; arIs92. Description: arIs92[egl-17p::NLS-CFP-LacZ + unc-4(+) + ttx-3::GFP]. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: X-rays Outcrossed: x10 Made by: Andrew S. Yoo Received: 12/13/06 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS3662 Species: Caenorhabditis elegans Genotype: dsl-1(ok810) IV. Description: WT phenotype. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: UV/TMP Outcrossed: x5 Made by: Ning Chen Received: 12/23/03 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS367 Species: Caenorhabditis elegans Genotype: evl-23(ar106)/dpy-13(e184) unc-24(e138) IV. Description: Heterozygotes are WT and segregate WT, Steriles with and Everted Vul and DpyUncs. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/11/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS368 Species: Caenorhabditis elegans Genotype: evl-13(ar107)/dpy-13(e184) unc-24(e138) IV. Description: Heterozygotes are WT and segregate WT, Steriles with and Everted Vul and DpyUncs. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/11/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS369 Species: Caenorhabditis elegans Genotype: evl-7(ar108)/dpy-13(e184) unc-24(e138) IV. Description: Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/18/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS370 Species: Caenorhabditis elegans Genotype: evl-12(ar109)/dpy-11(e224) unc-42(e270) V. Description: Heterozygotes are WT and segregate WT, Steriles with an Everted Vul and DpyUncs. Received new stock 10/2003. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/11/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS3754 Species: Caenorhabditis elegans Genotype: sel-13(ok303) III; arIs51 IV; sel-7(n1253) unc-3(e151) X. Description: arIs51[cdh-3::GFP]. sel-13=T04A8.10. Reported strong daf-c; raise at 15 C (personal communication to the CGC). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 04/05/05 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS3798 Species: Caenorhabditis elegans Genotype: arIs99. Description: arIs99 [dpy-7p::2Xnls::YFP]. Reference: Myers TR & Greenwald I, Dev Cell. 2005 Jan;8(1):117-23. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: X-ray Outcrossed: x4 Made by: Tasha Myers Received: 02/08/11 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS384 Species: Caenorhabditis elegans Genotype: fos-1(ar105)/dpy-11(e224) unc-42(e270) V. Description: Heterozygotes are WT and segregate WT, Steriles with an Everted Vul and DpyUncs. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. ar105 previously called evl-5(ar105). Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/15/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS387 Species: Caenorhabditis elegans Genotype: unc-36(e251) evl-14(ar112)/dpy-17(e164) unc-32(e189) III. Description: Heterozygotes are WT and segregate WT, DpyUncs and Sterile Unc-36 which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 07/31/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS401 Species: Caenorhabditis elegans Genotype: let-418(ar113)/unc-46(e177) dpy-11(e224) V. Description: Heterozygotes are WT and segregate WT, Steriles with an Everted Vul and DpyUncs. ar113 pka evl-11(ar113). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/11/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS402 Species: Caenorhabditis elegans Genotype: let-418(ar114)/unc-46(e177) dpy-11(e224) V. Description: Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted vulva. ar114 pka evl-11(ar114). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/18/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS404 Species: Caenorhabditis elegans Genotype: evl-4(ar116)/dpy-10(e128) unc-4(e120) II. Description: Heterozygotes are WT and segregate WT, Steriles with an Everted Vul and DpyUncs. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/15/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS408 Species: Caenorhabditis elegans Genotype: evl-2(ar119)/unc-85(e1414) dpy-10(e128) II. Description: Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted Vulva. CGC rec'd new stock from Iva Greenwald 5/2000. The unc-85 dpy-10 chromosome had been lost from this stock; for some reason it was difficult to maintain as a heterozygote. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x Made by: Geraldine Seydoux Received: 05/08/00 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS416 Species: Caenorhabditis elegans Genotype: evl-6(ar120)/dpy-13(e184) unc-24(e138) IV. Description: Heterozygotes are WT (Dpyish) and segregate WT (Dpyish), DpyUncs and Steriles which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/18/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS419 Species: Caenorhabditis elegans Genotype: evl-3(ar118)/dpy-10(e128) unc-4(e120) II; him-5(e1467) V. Description: Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted vulva. Throws males. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/18/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS422 Species: Caenorhabditis elegans Genotype: evl-21(ar122) unc-36(e251)/unc-93(e1500) dpy-17(e164) III. Description: Heterozygotes are WT and segregate WT, DpyUncs and Sterile Unc-36 which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/05/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS4249 Species: Caenorhabditis elegans Genotype: unc-4(e120) II; arIs82. Description: arIs82 [lin-12::GFP + egl-17p::lacZ + unc-4(+)]. Reference: Shaye DD, Greenwald I. Nature. 2002 Dec 12;420(6916):686-90. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x4 Made by: Dan Shaye Received: 03/20/13 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS431 Species: Caenorhabditis elegans Genotype: evl-24(ar124)/dpy-13(e184) unc-24(e138) IV. Description: Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/25/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS433 Species: Caenorhabditis elegans Genotype: evl-4(ar101)/dpy-10(e128) unc-4(e120) II. Description: Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/18/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS4335 Species: Caenorhabditis elegans Genotype: arIs41 II. Description: arIs41 [lin-12::GFP + rol-6(su1006)]. Rollers. Reference: Levitan D, Greenwald I. Development. 1998 Aug;125(16):3101-9. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Diane Levitan Received: 03/20/13 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS445 Species: Caenorhabditis elegans Genotype: lin-25(ar90) V/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Uncs, dead eggs, and Egl. lin-25(ar90) is a probable null mutation. Hermaphrodites are completely unable to lay eggs. Lineage defects in VPCs. Amber mutation (codon 197); amber suppressable. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x5 Made by: Simon Tuck Received: 03/06/01 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: GS454 Species: Caenorhabditis elegans Genotype: evl-9(ar121)/dpy-5(e61) unc-13(e51) I. Description: Heterozygotes are WT and segregate WT, DpyUncs and Steriles which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/18/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS457 Species: Caenorhabditis elegans Genotype: evl-18(ar117) dpy-17(e164)/unc-36(e251) III. Description: Heterozygotes are WT and segregate WT, Uncs and Sterile Dpys which have an everted vulva. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/04/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS460 Species: Caenorhabditis elegans Genotype: evl-15(ar126) dpy-20(e1282)/unc-26(e205) IV. Description: Heterozygotes are WT and segregate WT, Uncs and DpySteriles with an everted vulva. Recombines. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Geraldine Seydoux Received: 08/15/97 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS4815 Species: Caenorhabditis elegans Genotype: arIs98. Description: arIs98 [apx-1p::2xNLS::YFP + ceh-22p::GFP + dpy-20(+)]. References: Li J, Greenwald I. Curr Biol. 2010 Oct 26;20(20):1875-9. Karp X, Greenwald I. Proc Natl Acad Sci USA. 2013 Feb 5;110(6):2181-6. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x10 Made by: Min Sung Choi Received: 03/20/13 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS4892 Species: Caenorhabditis elegans Genotype: arIs131 III. Description: arIs131 [lag-2p::2xNLS::YFP + ceh-22p::GFP + pha-1(+)] III. References: Li J, Greenwald I. Curr Biol. 2010 Oct 26;20(20):1875-9. Zhang X, Greenwald I. Genetics. 2011 Aug;188(4):847-58. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x6 Made by: Ji Li&Xinyong Zhang Received: 03/20/13 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS5231 Species: Caenorhabditis elegans Genotype: arIs116 X. Description: arIs116 [lst-5p::2xNLS::YFP + ttx-3p::GFP + pha-1(+)] X. References: Li J, Greenwald I. Curr Biol. 2010 Oct 26;20(20):1875-9. Karp X, Greenwald I. Proc Natl Acad Sci USA. 2013 Feb 5;110(6):2181-6. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x>6 Made by: Min Sung Choi Received: 03/20/13 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS528 Species: Caenorhabditis elegans Genotype: lin-25(sy29) V/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Uncs, dead eggs, and Egl. lin-25(sy29) is a non-null allele. Hermaphrodites are 100% Egl but some eggs are seen on plates. Results from a missense mutation in codon 103. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x Made by: Chamberlin/Sternberg Received: 03/06/01 Umea University, Vasterbotten, Sweden ----------------------------------------------------------------------------- Strain: GS60 Species: Caenorhabditis elegans Genotype: unc-32(e189) lin-12(n676n930) III. Description: Unc. Temperature sensitive hypomorphic lin-12 allele. At restrictive temperature of 25C: 100% Egl in unc-32 background (95% Egl without unc-32); 30% have 2 anchor cells, some proximal mitosis, abnormal vulval lineages but has opening; leaks some eggs; mates with males. At permissive temperature of 15C: 93% Egl(+) and 7% Egl(-) [lin-12(d)-like: no anchor cell and no vulval opening]. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x>2 Made by: Iva Greenwald Received: 01/25/94 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS6107 Species: Caenorhabditis elegans Genotype: arIs107. Description: arIs107 [mir-61p::2xNLS::YFP + ttx-3p::GFP + pha-1(+)]. References: Yoo AS, Greenwald I. Science. 2005 Nov 25;310(5752):1330-3. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x>6 Made by: Andrew Yoo Received: 03/20/13 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS7637 Species: Caenorhabditis elegans Genotype: cyk-1(or596) III; arIs198. Description: Maintain at 15C. cyk-1(or596) is temperature-sensitive embryonic lethal. arIs198 [glt-3p::CFP + glt-3p::LifeAct::TagRFP]; expresses cytoplasmic CFP and LifeAct::TagRFP in the excretory canal cell under control of the glt-3 promoter. Mutagen: ENU Outcrossed: x7x Made by: Dan Shaye Received: 12/14/17 University of Illinois, Chicago, IL ----------------------------------------------------------------------------- Strain: GS7638 Species: Caenorhabditis elegans Genotype: exc-6(gk386) cyk-1(or596) III; arIs198. Description: Maintain at 15C. cyk-1(or596) is temperature-sensitive embryonic lethal. arIs198 [glt-3p::CFP + glt-3p::LifeAct::TagRFP]; expresses cytoplasmic CFP and LifeAct::TagRFP in the excretory canal cell under control of the glt-3 promoter. Shortened excretory canals. Mutagen: Outcrossed: x>3 Made by: Dan Shaye Received: 12/14/17 University of Illinois, Chicago, IL ----------------------------------------------------------------------------- Strain: GS776 Species: Caenorhabditis elegans Genotype: unc-32(e189) lin-12(n676n930) III; unc-42(e270) sel-11(ar39) V. Description: Unc. At 25C ar39 recessively suppresses the Egl phenotype of n676n930. At 15C a high percentage of hermaphrodites have a 0 AC-Egl phenotype. ar39 recessively suppresses vulval lineage defects and proximal mitosis. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x>2 Made by: Meera Sundaram Received: 01/25/94 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS7960 Species: Caenorhabditis elegans Genotype: exc-6(gk386) cyk-1(or596) III; inft-2(ok1296) V; arIs198. Description: Maintain at 15C. cyk-1(or596) is temperature-sensitive embryonic lethal. arIs198 [glt-3p::CFP + glt-3p::LifeAct::TagRFP]; expresses cytoplasmic CFP and LifeAct::TagRFP in the excretory canal cell under control of the glt-3 promoter. Shortened excretory canals. Strain is quite sick even at the permissive temperature. Mutagen: Outcrossed: x>3 Made by: Dan Shaye Received: 12/14/17 University of Illinois, Chicago, IL ----------------------------------------------------------------------------- Strain: GS807 Species: Caenorhabditis elegans Genotype: unc-32(e189) lin-12(n676n930) III; sqt-3(sc8) sel-1(e1948) V. Description: RollerUnc. At 25C e1948 recessively suppresses the Egl phenotype of n676n930. At 15C a high percentage of hermaphrodites have a 0 AC-Egl phenotype. e1948 recessively suppresses vulval lineage defects and proximal mitosis. sc8 previously called rol-4(sc8). See also WBPaper00002448. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x>2 Made by: Iva Greenwald Received: 01/25/94 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS8190 Species: C. elegans Genotype: arTi85. Description: arTi85 [lin-31p::ERK::KTR::mClover::T2A::mCherry::his-11::unc-54 3'UTR + rps-27p::NeoR::unc-54 3'UTR]. Superficially wild-type. arTi85 transgene is a single-copy transposon insertion expressing a fluorescent protein (ERK::KTR::mClover) that reports MPK-1 kinase activity in vulval precursor cells (VPCs). A nuclear histone marker is co-expressed (mCherry::H2B). Reference: de la Cova C, et al. Developmental Cell. 2017 Vol. 42(5):542-553. Mutagen: Mos1 transposon Outcrossed: x0 Made by: Claire de la Cova Received: 05/11/18 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS8255 Species: C. elegans Genotype: arTi101. Description: arTi101 [lin-31p::ERK::KTR(S43A, T55A, S62A)::mClover::T2A::mCherry::his-11::unc-54 3'UTR + rps-27p::NeoR::unc-54 3'UTR]. Superficially wild-type. arTi101 transgene is a single-copy transposon insertion expressing a mutant, unphosphorylated form of a fluorescent protein (ERK::KTR::mClover) that reports MPK-1 kinase activity in vulval precursor cells (VPCs). A nuclear histone marker is co-expressed (mCherry::H2B). Use arTi101 as a negative control for transgene arTi85. Reference: de la Cova C, et al. Developmental Cell. 2017 Vol. 42(5):542-553. Mutagen: Mos1 transposon Outcrossed: x0 Made by: Claire de la Cova Received: 05/11/18 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS8513 Species: C. elegans Genotype: arTi145 II. Description: arTi145 [ckb-3p::mCherry::his-58::unc-54 3'UTR] II. miniMos insertion with bright expression due to perdurance mediated by the histone; expressed in all cells from Z1/Z4 until somatic gonad blast cells divide, so labels the entire somatic gonad primordium in continuous L2 or dauer larvae. Reference: Attner et al., 2019, Current Biology 29, 1-7. Mutagen: Outcrossed: x0 Made by: Justin Benavidez Received: 09/05/19 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS8729 Species: C. elegans Genotype: arSi12. Description: arSi12 [mex-5p::ERK::KTR::GFP(smu-1 introns)::T2A::mCherry::his-11::tbb-2 3'UTR]. arSi12 is a single-copy CRISPR/Cas9-engineered insertion expressing a fluorescent protein (ERK::KTR::GFP) that reports MPK-1 kinase activity in the germline. A nuclear histone marker is co-expressed (mCherry::H2B). Reference: de la Cova C, et al. Developmental Cell. 2017 Vol. 42(5):542-553. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Claire de la Cova Received: 05/11/18 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS8752 Species: C. elegans Genotype: arSi15. Description: arSi15 [mex-5p::ERK::KTR(S43A, T55A, S62A)::GFP(smu-1 introns)::T2A::mCherry::his-11::tbb-2 3'UTR]. arSi15 is a single-copy CRISPR/Cas9-engineered insertion expressing a mutant, unphosphorylated form of a fluorescent protein (ERK::KTR::GFP) that reports MPK-1 kinase activity in the germline. A nuclear histone marker is co-expressed (mCherry::H2B). Use arSi15 as a negative control for transgene arSi12. Reference: de la Cova C, et al. Developmental Cell. 2017 Vol. 42(5):542-553. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x0 Made by: Claire de la Cova Received: 05/11/18 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS878 Species: Caenorhabditis elegans Genotype: unc-32(e189) lin-12(n676n930) III; lon-3(e2175) sel-10(ar41) V. Description: Long. Unc. At 15C ar41 suppresses the 2 AC (anchor cell) phenotype of n676n930. (Strain GS878 does not contain sel(arX) and therefore does not suppress the Egl phenotype at 25C.) See also WBPaper00002966. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x>2 Made by: Meera Sundaram Received: 01/25/94 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS883 Species: Caenorhabditis elegans Genotype: dpy-5(e61) sel(ar40) I; unc-32(e189) lin-12(n676n930) III. Description: DpyUnc. ar40 is a semi-dominant suppressor. At 25C ar40 suppresses the Egl phenotype of ne676n930. At 15C a high percentage of hermaphrodites have a 0 AC-Egl phenotype. ar40 suppresses proximal mitosis. ar40 does not suppress vulval lineage defects. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x>2 Made by: Meera Sundaram Received: 01/25/94 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS8949 Species: C. elegans Genotype: hlh-2(ar623[gfp::hlh-2]) I. Description: GFP tag inserted into endogenous hlh-2 locus produces a fully-functional GFP-tagged form of HLH-2. Reference: Attner et al., 2019, Current Biology 29, 1-7. Mutagen: Outcrossed: x0 Made by: Justin Benavidez Received: 09/05/19 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS925 Species: Caenorhabditis elegans Genotype: let-54(s44) unc-22(s7) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Unc, dead eggs and lethal Twitchers. Lethal early larval (L1-L2). Maintain by picking Unc. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Simon Tuck Received: 01/10/94 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS9402 Species: C. elegans Genotype: arTi362 [rps-27p::TIR1(lox2272-flexon-lox2272)::unc-54 3'UTR + HygroR] V. Description: arTi362 [rps-27p::TIR1(lox2272-flexon-lox2272)::unc-54 3'UTR + HygroR] V (-19.98). The first intron of TIR1 was replaced with a Flexon containing two lox2272 sites. TIR1 is expressed at a high level in lineages where Cre is expressed. Reference: Wittes J & Greenwald I. (2024). New Flexon-based reagents for tissue-specific Auxin-Inducible Degradation and for characterizing Cre and Flp drivers in C. elegans. microPublication Biology. 10.17912/micropub.biology.001315. Mutagen: miniMos Outcrossed: x0 Made by: Julia Wittes Received: 09/19/24 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS9407 Species: C. elegans Genotype: arTi361 [rps-27p::GFP(lox2272-flexon-lox2272)::H2B::unc-54 3'UTR + NeoR] I. Description: arTi361 [rps-27p::GFP(lox2272-flexon-lox2272)::H2B::unc-54 3'UTR + NeoR] I (-12.74). First intron of GFP is replaced with a Flexon containing two lox2272 sites. GFP::H2B is expressed at a high level in lineages where Cre is expressed with a second transgene. Transgene maps to -12.74 (I). Reference: Wittes J & Greenwald I. (2024). New Flexon-based reagents for tissue-specific Auxin-Inducible Degradation and for characterizing Cre and Flp drivers in C. elegans. microPublication Biology. Mutagen: miniMos Outcrossed: x0 Made by: Justin Shaffer and Henry Kim Received: 09/19/24 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS9801 Species: C. elegans Genotype: rde-1(ar660) V. Description: rde-1(ar660)[rde-1(lox2272-flexon-lox2272)]. 9th intron of rde-1 replaced with a flexon - an artificial exon flanked by artificial introns that each contain a lox2272 site (a flexon). Severely reduces rde-1 function, function can be restored upon excision of the flexon by Cre recombinase. Reference: Shaffer JM & Greenwald I. Proc Natl Acad Sci U S A. 2022 Jan 18;119(3):e2117451119. doi: 10.1073/pnas.2117451119. PMID: 35027456. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Justin Shaffer Received: 07/12/22 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS9820 Species: C. elegans Genotype: arTi443 [rps-27p::TIR1(F79G)(lox2272-flexon-lox2272)::unc-54 3'UTR + HygroR] V. Description: arTi443 [rps-27p::TIR1(F79G)(lox2272-flexon-lox2272)::unc-54 3'UTR + HygroR] V (+21.99). The first intron of TIR1(F79G) was replaced with a Flexon containing two lox2272 sites. TIR1(F79G) is expressed at a high level in lineages where Cre is expressed with a second transgene. Reference: Wittes J & Greenwald I. (2024). New Flexon-based reagents for tissue-specific Auxin-Inducible Degradation and for characterizing Cre and Flp drivers in C. elegans. microPublication Biology. 10.17912/micropub.biology.001315. Mutagen: miniMos Outcrossed: x0 Made by: Julia Wittes Received: 09/19/24 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS9847 Species: C. elegans Genotype: arTi452 [rps-27p::GFP(LoxP-flexon-LoxP)::H2B::unc-54 3'UTR + NeoR] I. Description: arTi452 [rps-27p::GFP(LoxP-flexon-LoxP)::H2B::unc-54 3'UTR + NeoR] I (-4.81). First intron of GFP is replaced with a Flexon containing two loxP sites. GFP::H2B is expressed at a high level in lineages where Cre is expressed with a second transgene. Reference: Wittes J & Greenwald I. (2024). New Flexon-based reagents for tissue-specific Auxin-Inducible Degradation and for characterizing Cre and Flp drivers in C. elegans. microPublication Biology. 10.17912/micropub.biology.001315. Mutagen: miniMos Outcrossed: x0 Made by: Julia Wittes Received: 09/19/24 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GS9922 Species: C. elegans Genotype: arTi461 [rps-27p::GFP(FRT-flexon-FRT)::H2B::unc-54 3'UTR + NeoR] I. Description: arTi461 [rps-27p::GFP(FRT-flexon-FRT)::H2B::unc-54 3'UTR + NeoR] I (-3.80). The first intron of GFP was replaced with a Flexon containing two FRT sites. GFP::H2B is expressed at a high level in lineages where Flp recombiase is expressed with a second transgene. Flexon contains two FRT sites. Reference: Wittes J & Greenwald I. (2024). New Flexon-based reagents for tissue-specific Auxin-Inducible Degradation and for characterizing Cre and Flp drivers in C. elegans. microPublication Biology. 10.17912/micropub.biology.001315. Mutagen: miniMos Outcrossed: x0 Made by: Julia Wittes Received: 09/19/24 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: GT330 Species: C. elegans Genotype: aSi8 II; unc-119(ed3) III. Description: aSi8 [lox2272::Cbr-unc-119(+)::lox2272::mec-7p:: NLS::GCaMP7s::egl-13-NLS::SL2::NLS::mScarlet-I:: egl-13-NLS] II. mec-7 promoter driving nuclear-localized expression of GCaMP7f in ALM, PLM, AVM & PVM neurons. Reference: Ding J, et al. GE (Bethesda). 2023 Sep 30;13(10):jkad183. doi: 10.1093/g3journal/jkad183. PMID: 37565483. Mutagen: Outcrossed: x0 Made by: Hang Lu Received: 12/05/23 Georgia Institute of Technology ----------------------------------------------------------------------------- Strain: GT332 Species: Caenorhabditis elegans Genotype: aSi10 II; unc-119(ed3) III. Description: aSi10 [lox2272 Cbr-unc-119(+) lox2272 + loxP::unc-54 3’UTR::Split 3’ HygR::tjp2a_guide::Split 3’ mScarlet-I::egl-13nls::tbb-2 3’UTR] II. Strain contains a specialized safe harbor transgene landing pad for integration of promoters to drive mScarlet. Reference: Stevenson ZC, et al. bioRxiv 2022.10.30.514301; doi: https://doi.org/10.1101/2022.10.30.514301. Paper accepted at eLife. Mutagen: Outcrossed: x0 Made by: Dhaval Patel Received: 04/27/23 University of Oregon, Eugene, OR ----------------------------------------------------------------------------- Strain: GT337 Species: Caenorhabditis elegans Genotype: aSi13 II; unc-119(ed3) III. Description: aSi13 [lox2272 + loxN 3' (delta)Cbr-unc-119(+) + 3' (delta)mNeonGreen::PEST] aSi14[lox2272 + loxP 3’ (delta)HygR + 3’ (delta)mScarlet-I::PEST] II. Unc. Strain contains a set of dual specialized safe harbor transgene landing pads for integration of promoters: one driving mScarlet and rescuing hygromycin resistance upon integration, the other driving mNeonGreen and rescuing the unc phenotype upon integration. Reference: Stevenson ZC, et al. bioRxiv 2022.10.30.514301; doi: https://doi.org/10.1101/2022.10.30.514301. Paper accepted at eLife. Mutagen: Outcrossed: x0 Made by: Dhaval Patel Received: 04/27/23 University of Oregon, Eugene, OR ----------------------------------------------------------------------------- Strain: GT347 Species: C. elegans Genotype: aSi23 II; unc-119(ed3) III. Description: aSi23 [lox2272::Cbr-unc-119(+)::lox2272::mec-7p:: NLS::GCaMP7f::egl-13-NLS::SL2::NLS::mScarlet-I:: egl-13-NLS] II. mec-7 promoter driving nuclear-localized expression of GCaMP7f in ALM, PLM, AVM & PVM neurons. Reference: Ding J, et al. GE (Bethesda). 2023 Sep 30;13(10):jkad183. doi: 10.1093/g3journal/jkad183. PMID: 37565483. Mutagen: Outcrossed: x0 Made by: Hang Lu Received: 12/05/23 Georgia Institute of Technology ----------------------------------------------------------------------------- Strain: GT350 Species: C. elegans Genotype: aSi26 II; unc-119(ed3) III. Description: aSi26 [lox2272::Cbr-unc-119(+)::lox2272::mec-7p:: NLS::GCaMP7s::ras-2-CAAX::SL2::mScarlet-I::ras-2-CAAX] II. mec-7 promoter driving membrane-localized expression of GCaMP7f in ALM, PLM, AVM & PVM neurons. Reference: Ding J, et al. GE (Bethesda). 2023 Sep 30;13(10):jkad183. doi: 10.1093/g3journal/jkad183. PMID: 37565483. Mutagen: Outcrossed: x0 Made by: Hang Lu Received: 12/05/23 Georgia Institute of Technology ----------------------------------------------------------------------------- Strain: GT372 Species: C. elegans Genotype: aSi31 II; unc-119(ed3) III Description: aSi31 [lox2272::Cbr-unc-119(+)::lox2272::mec-7p:: NLS::GCaMP7f::ras-2-CAAX::SL2::mScarlet-I::ras-2-CAAX] II. mec-7 promoter driving membrane-localized expression of GCaMP7f in ALM, PLM, AVM & PVM neurons. Reference: Ding J, et al. GE (Bethesda). 2023 Sep 30;13(10):jkad183. doi: 10.1093/g3journal/jkad183. PMID: 37565483. Mutagen: Outcrossed: x0 Made by: Hang Lu Received: 12/05/23 Georgia Institute of Technology ----------------------------------------------------------------------------- Strain: GT375 Species: C. elegans Genotype: aSi27 II; unc-119(ed3) III. Description: aSi27 [lox2272::Cbr-unc-119(+)::lox2272::mec-7p::GCaMP7s::SL2::mScarlet-I] II. mec-7 promoter driving expression of GCaMP7s in ALM, PLM, AVM & PVM neurons. Reference: Ding J, et al. GE (Bethesda). 2023 Sep 30;13(10):jkad183. doi: 10.1093/g3journal/jkad183. PMID: 37565483. Mutagen: Outcrossed: x0 Made by: Hang Lu Received: 12/05/23 Georgia Institute of Technology ----------------------------------------------------------------------------- Strain: GT377 Species: C. elegans Genotype: aSi36 II; unc-119(ed3) III. Description: aSi36 [lox2272::Cbr-unc-119(+)::lox2272::mec-7p::GCaMP7f::SL2::mScarlet-I] II. mec-7 promoter driving expression of GCaMP7f in ALM, PLM, AVM & PVM neurons. Reference: Ding J, et al. GE (Bethesda). 2023 Sep 30;13(10):jkad183. doi: 10.1093/g3journal/jkad183. PMID: 37565483. Mutagen: Outcrossed: x0 Made by: Hang Lu Received: 12/05/23 Georgia Institute of Technology ----------------------------------------------------------------------------- Strain: GV1 Species: Caenorhabditis elegans Genotype: ets-4(uz1) X. Description: Reference: Thyagarajan et al. PLoS Genet. 2010 Sep 16;6(9). pii: e1001125. Mutagen: EMS Outcrossed: x6 Made by: Adam Blaszczak Received: 11/08/10 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: GV796 Species: Caenorhabditis elegans Genotype: ets-4(ok165) lin-15B&lin-15A(n765) X; uzEx710. Description: uzEx710 [gly-19p::YFP::ets-4 + lin-15(+)]. Maintain by picking wild-type (non-Muv). Reference: Thyagarajan et al. PLoS Genet. 2010 Sep 16;6(9). pii: e1001125. Mutagen: Outcrossed: x Made by: Colin Thacker Received: 11/08/10 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: GV807 Species: Caenorhabditis elegans Genotype: ets-4(ok165) lin-15B&lin-15A(n765) X; uzEx721. Description: uzEx721 [rab-3p::YFP::ets-4 + lin-15(+)]. Maintain by picking wild-type (non-Muv). Reference: Thyagarajan et al. PLoS Genet. 2010 Sep 16;6(9). pii: e1001125. Mutagen: Outcrossed: x Made by: Colin Thacker Received: 11/08/10 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: GV812 Species: Caenorhabditis elegans Genotype: ets-4(ok165) lin-15B&lin-15A(n765) X; uzEx726. Description: uzEx726 [lin-15(+)]. Maintain by picking wild-type (non-Muv). Reference: Thyagarajan et al. PLoS Genet. 2010 Sep 16;6(9). pii: e1001125. Mutagen: Outcrossed: x Made by: Colin Thacker Received: 11/08/10 University of Utah, Salt Lake City, UT ----------------------------------------------------------------------------- Strain: GW1028 Species: C. elegans Genotype: met-2(n4256) set-25(n5021) III; rrrSi192 [mex-5p::GFP::H2B::tbb-2 3'UTR + unc-119(+)] II. Description: Worms are slow growing, with reduced brood size and become sterile at elevated temperatures. met-2 and set-25 mutants co-segregate with ~ 20cM distance. Express GFP::H2B in germline and early embryos. Might still carry unc-119(ed3) in background. Reference: Zeller P, et al. Nat Genet. 2016 Nov;48(11):1385-1395. doi: 10.1038/ng.3672. PMID: 27668659. Mutagen: Outcrossed: xx2 Made by: Veronique Kalck Received: 06/11/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW1119 Species: C. elegans Genotype: lsm-8 (xe17[myo-2p::mCherry::unc-54 3'UTR]) IV/nT1 [qIs51] (IV;V); pkIs1582 V/nT1 [qIs51] (IV;V). Description: pkIs1582 [let-858::GFP + rol-6(su1006)] V. Homozygous lethal lsm-8 deletion balanced by GFP-marked nT1 translocation. xe17 generated by CRISPR/Cas9-engineered replacement of the gene with a red pharyngeal marker. lsm-8 heterozygotes are wild-type (will roll in this case because of pkIs1582) green & red pharynx, and will segregate rolling heterozygotes (green & red pharynx), arrested nT1[qIs51] aneuploids (only green pharynx), and lsm-8 homozygotes (only red pharynx). Homozygous nT1[qIs51] inviable. Pick rollers with green & red pharynx and check for correct segregation of progeny to maintain. Reference: Mattout A, et al. Nat Cell Biol. 2020 May;22(5):579-590. PMID: 32251399 Mutagen: Crispr/Cas9 Outcrossed: x4 Made by: Florian Aeschlimann Received: 05/14/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW1203 Species: C. elegans Genotype: met-2(n4256) set-25(n5021) III; bcIs39 [lim-7p::ced-1::GFP + lin-15(+)] V. Description: Worms are slow growing, with reduced brood size and become sterile at elevated temperatures. CED-1::GFP used to detect apoptotic cells in germline. Reference: Zeller P, et al. Nat Genet. 2016 Nov;48(11):1385-1395. doi: 10.1038/ng.3672. PMID: 27668659. Mutagen: Outcrossed: xx4 Made by: Peter Zeller Received: 06/11/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW1262 Species: C. elegans Genotype: xeSi302 II; gwIs114. Description: xeSi302 [nhx-2p::npp-9::GFP::BLRP::3xFLAG::unc-54 3'UTR + Cbr-unc-119(+)] II. gwIs114 [hsp-16.2p::hlh-1 + rol6(su1006)]. Intestine-specific expression of nuclear GFP reporter. Rollers have heat-shock-inducible expression of hlh-1 transcription factor. gwIs114 was generated using constructs provided by Michael W. Krause`s lab (NIDDK). Reference: Gonzalez-Sandoval A, et al. Cell. 2015 Dec 3;163(6):1333-47. doi: 10.1016/j.cell.2015.10.066. PMID: 26607792. Mutagen: MosSCI Outcrossed: x Made by: Adriana Gonzalez Received: 06/11/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW1394 Species: C. elegans Genotype: gwIs39 III; bqSi225 IV; gwIs59. Description: gwIs39 [baf-1::gfp-lacI::let-858 3'UTR + vit-5::GFP] III. bqSi225 [emr-1p::emr-1::mCherry + unc-119(+)] IV. gwIs59 [pha-4::mCherry::256xLacO::4xLexA + unc-119(+)]. Superficially wild-type. Express ubiquitous EMR-1::mCherry at the nuclear periphery. Expresses GFP-LacI from early embryogenesis and throughout development, which forms a small spot at the lacO array. Worms have red intestine (all stages) and green intestine (from late L4 stage). Reference: Harr JC, et al. Genes Dev. 2020 Apr 1;34(7-8):560-579. PMID: 32139421. Mutagen: Outcrossed: x Made by: Jennifer Harr Received: 06/11/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW1419 Species: C. elegans Genotype: met-2(gw1419[met-2::FLAG::TEV::mCherry]) III. Description: Superficially wild-type. Endogenously tagged met-2 locus, with MET-2::mCherry signal detectable in all germline and somatic tissues. Reference: Delaney CE, et al. J Cell Biol. 2019 Mar 4;218(3):820-838. PMID: 30737265 Mutagen: Crispr/Cas9 Outcrossed: x4 Made by: Micol Guidi Received: 05/14/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW1480 Species: C. elegans Genotype: bqsi433 II; bqSi495 IV; ygIs1. Description: bqsi433 [hsp16.41p::FRT::mCherry::his-58::FRT::dam::emr-1 + unc-119(+)] II. bqSi495 [myo-3p::FLP::D5 + unc-119(+)] IV. ygIs1 [baf-1p::GFP::lamin(wt) + unc-119(+)]. Strain used to perform muscle specific EMR-DamID, to map lamina-associated domains (LADs). Strain also has low level over-expression of GFP::LMN-1. Might still carry unc-119(ed3) or (ed9) in background. Reference: Harr JC, et al. Genes Dev. 2020 Apr 1;34(7-8):560-579. PMID: 32139421 Mutagen: Outcrossed: x2 Made by: Jennifer Harr Received: 05/14/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW1481 Species: C. elegans Genotype: bqSi447 II; bqSi495 IV; ygIs1. Description: bqSi447 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::dam + unc-119(+)] II. bqSi495 [myo-3p::FLP::D5 + unc-119(+)] IV. ygIs1 [baf-1p::GFP::lamin(wt) + unc-119(+)]. Strain expressing muscle specific DAM::GFP, used as a control for muscle specific EMR-DamID. Strain also has low level over-expression of GFP::LMN-1. Might still carry unc-119(ed3) or (ed9) in background. Reference: Harr JC, et al. Genes Dev. 2020 Apr 1;34(7-8):560-579. PMID: 32139421 Mutagen: Outcrossed: x2 Made by: Jennifer Harr Received: 05/14/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW1482 Species: C. elegans Genotype: bqsi433 II; bqSi495 cec-4(ok3124) IV; ygIs1. Description: bqsi433 [hsp16.41p::FRT::mCherry::his-58::FRT::dam::emr-1 + unc-119(+)] II. bqSi495 [myo-3p::FLP::D5 + unc-119(+)] IV. ygIs1 [baf-1p::GFP::lamin(wt) + unc-119(+)]. Strain used to perform muscle specific EMR-DamID, to map lamina-associated domains (LADs). Strain also has low level over-expression of GFP::LMN-1. Strain also has cec-4 deletion. Might still carry unc-119(ed3) or (ed9) in background. Reference: Harr JC, et al. Genes Dev. 2020 Apr 1;34(7-8):560-579. PMID: 32139421 Mutagen: Outcrossed: x2 Made by: Jennifer Harr Received: 05/14/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW1483 Species: C. elegans Genotype: bqSi447 II; bqSi495 cec-4(ok3124) IV; ygIs1. Description: bqSi447 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::dam + unc-119(+)] II. bqSi495 [myo-3p::FLP::D5 + unc-119(+)] IV. ygIs1 [baf-1p::GFP::lamin(wt) + unc-119(+)]. Strain expressing muscle specific DAM::GFP, used as a control for muscle specific EMR-DamID. Strain also has low level over-expression of GFP::LMN-1. Strain also has cec-4 deletion. Might still carry unc-119(ed3) or (ed9) in background. Reference: Harr JC, et al. Genes Dev. 2020 Apr 1;34(7-8):560-579. PMID: 32139421 Mutagen: Outcrossed: x2 Made by: Jennifer Harr Received: 05/14/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW1485 Species: C. elegans Genotype: bqSi447 II; bqSi495 IV; ygIs2. Description: bqSi447 [hsp16.41p::FRT::mCherry::his-58::FRT::GFP::dam + unc-119(+)] II. bqSi495 [myo-3p::FLP_D5 + unc-119(+)] IV. ygIs2 [baf-1::GFP::lmn-1(Y59C) + unc-119(+)]. Strain expressing muscle specific DAM::GFP, used as a control for muscle specific EMR-DamID. Strain also has low level over-expression of GFP::LMN-1 (Y59C mutation). Might still carry unc-119(ed3) in background. Reference: Harr JC, et al. Genes Dev. 2020 Apr 1;34(7-8):560-579. PMID: 32139421. Mutagen: Outcrossed: x Made by: Jennifer Harr Received: 06/11/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW1539 Species: C. elegans Genotype: gwSi34 II; met-2(gw1419[met-2::FLAG::TEV::mCherry]) III. Description: gwSi34 [lem-2p::lem-2::GFP-3xFlag::lem-2 3'UTR] II. Superficially wild-type. Endogenously tagged met-2 locus. LEM-2::GFP is detectable at the nuclear periphery, and MET-2::mCherry signal detectable throughout nucleoplasm of all germline and somatic tissues. Reference: Delaney CE, et al. J Cell Biol. 2019 Mar 4;218(3):820-838. PMID: 30737265 Mutagen: Outcrossed: x Made by: Micol Guidi Received: 05/14/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW1562 Species: C. elegans Genotype: lin-65(gw1465) I. Description: CRISPR-engineered whole-gene deletion of lin-65. Worms are slow growing with reduced brood size and become sterile at elevated temperatures. Reference: Delaney CE, et al. J Cell Biol. 2019 Mar 4;218(3):820-838. PMID: 30737265 Mutagen: Crispr/Cas9 Outcrossed: x4 Made by: Colin Delaney Received: 05/14/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW1599 Species: C. elegans Genotype: met-2(n4256) set-25(n5021) III; opIs263. Description: opIs263 [rpa-1p::rpa-1::YFP + unc-119(+)]. Worms are slow growing with reduced brood size and become sterile at elevated temperatures. Express RPA-1::YFP in both germline and somatic tissues. Reference: Padeken J, et al. Genes Dev. 2019 Apr 1;33(7-8):436-451. PMID: 30804228 Mutagen: Outcrossed: x2 Made by: Stephen Methot Received: 05/14/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW1602 Species: C. elegans Genotype: met-2(n4256) set-25(n5021) III; lsIs17. Description: lsIs17 [pie-1p::GFP::pcn-1(W03D2.4) + unc-119(+)]. Worms are slow growing with reduced brood size and become sterile at elevated temperatures. Express GFP::PCN-1 in dividing cells of germline, embryos and larvae. Reference: Padeken J, et al. Genes Dev. 2019 Apr 1;33(7-8):436-451. PMID: 30804228 Mutagen: Outcrossed: x2 Made by: Stephen Methot Received: 05/14/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW1618 Species: C. elegans Genotype: lin-65(gw1578[lin-65::FLAG::TEV::GFP]) I. Description: Superficially wild-type. Endogenously tagged lin-65 locus, with LIN-65::GFP signal detectable in all germline and somatic tissues. Reference: Delaney CE, et al. J Cell Biol. 2019 Mar 4;218(3):820-838. PMID: 30737265 Mutagen: Crispr/Cas9 Outcrossed: x4 Made by: Colin Delaney Received: 05/14/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW1621 Species: C. elegans Genotype: lin-65(gw1578[lin-65::FLAG::TEV::GFP]) I; met-2(gw1419[met-2::FLAG::TEV::mCherry]) III. Description: Superficially wild-type. Endogenously tagged met-2 and lin-65 loci. MET-2::mCherry and LIN-65::GFP signal are detectable in all germline and somatic tissues. Reference: Delaney CE, et al. J Cell Biol. 2019 Mar 4;218(3):820-838. PMID: 30737265 Mutagen: Crispr/Cas9 Outcrossed: x4 Made by: Colin Delaney Received: 05/14/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW1623 Species: C. elegans Genotype: arle-14(gw1623[GFP::TEV::3xFLAG::arle-14]) met-2(gw1419[met-2::FLAG::TEV::mCherry]) III. Description: Superficially wild-type. Endogenously tagged met-2 and arle-14 loci. MET-2::mCherry and GFP::ARLE-14 signal are detectable in all germline and somatic tissues. Reference: Delaney CE, et al. J Cell Biol. 2019 Mar 4;218(3):820-838. PMID: 30737265 Mutagen: Crispr/Cas9 Outcrossed: x4 Made by: Colin Delaney Received: 05/14/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW215 Species: C. elegans Genotype: hpl-2(tm1489) III; gwIs4 X. Description: gwIs4 [myo-3p::RFP + baf-1::GFP-lacI:::let-858 3'UTR] X. Worms are slow growing, with reduced brood size and become sterile at elevated temperatures. Expresses GFP-LacI from early embryogenesis and throughout development, which forms a small spot at the lacO array. RFP expression in muscles. Reference: Gonzalez-Sandoval A, et al. Cell. 2015 Dec 3;163(6):1333-47. doi: 10.1016/j.cell.2015.10.066. PMID: 26607792. Mutagen: Outcrossed: xx4 Made by: Peter Meister Received: 06/11/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW304 Species: C. elegans Genotype: unc-119(ed3) III; gwIs28. Description: gwIs28 [myo-3::mCherry + 256xlacO + unc-119(+)]. Superficially wild-type. Contains a small lacO array co-integrated with a muscle specific mCherry reporter (~10x smaller than the gwIs4 array). Reference: Meister P, et al. Genes Dev. 2010 Apr 15;24(8):766-82. PMID: 20395364 Mutagen: Bombardment Outcrossed: x4 Made by: Peter Meister Received: 05/14/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW398 Species: C. elegans Genotype: gwIs39 gwIs34 unc-119(ed3) III. Description: gwIs39 [baf-1::gfp-lacI::let-858 3'UTR + vit-5::GFP] III. gwIs34 [myo-3::mCherry + 256xlacO + unc-119(+)] III. Superficially wild-type. Expresses GFP-LacI throughout development from early embryogenesis, forming a small spot at the lacO array. Worms have red muscle (from L1 stage) and green intestine (from late L4 stage). Reference: Meister P, et al. Genes Dev. 2010 Apr 15;24(8):766-82. PMID: 20395364 Mutagen: Bombardment Outcrossed: x4 Made by: Peter Meister Received: 05/14/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW421 Species: C. elegans Genotype: gwIs39 III; gwIs58. Description: gwIs39 [baf-1::gfp-lacI::let-858 3'UTR + vit-5::GFP] III. gwIs58 [hsp-16.2p::mCherry::256xLacO::4xLexA + unc-119(+)]. Small transgene/large array. Superficially wild-type. Expresses GFP-LacI from early embryogenesis and throughout development, which forms a small spot at the lacO array. Worms have green intestine (from late L4 stage). Might still carry unc-119(ed3) in background. Reference: Rohner S, et al. J Cell Biol. 2013 Mar 4;200(5):589-604. doi: 10.1083/jcb.201207024. PMID: 23460676 Mutagen: Bombardement Outcrossed: xx2 Made by: Sabine Rohner Received: 06/11/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW513 Species: C. elegans Genotype: gwIs39 III; ygIs2; caIs3. Description: gwIs39 [baf-1::gfp-lacI::let-858 3'UTR + vit-5::GFP] III. ygIs2 [baf-1::GFP::lmn-1(Y59C) + unc-119(+)]. caIs3 [pha-4::lacZ + rol-6(su1006)]. Rollers. Strain expressing low level of GFP::LMN-1 (carrying the Y59C mutation). Expresses GFP-LacI from early embryogenesis and throughout development, which forms a small spot at the lacO array. Worms have red muscle (from L1 stage) and green intestine (from late L4 stage). Reference: Mattout A, et al. Curr Biol. 2011 Oct 11;21(19):1603-14. doi: 10.1016/j.cub.2011.08.030. PMID: 21962710 Mutagen: Outcrossed: x Made by: Brietta Pike Received: 06/11/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW566 Species: C. elegans Genotype: gwIs39 III; gwIs4 X. Description: gwIs39 [baf-1::gfp-lacI::let-858 3'UTR + vit-5::GFP] III. gwIs4 [baf-1p::GFP-lacI::let-858 3’UTR + myo-3p::RFP] X. Superficially wild-type. Expresses GFP-LacI throughout development from early embryogenesis, forming a large spot at the lacO array. Worms have red muscle (from L1 stage) and green intestine (from late L4 stage). Reference: Towbin BD, et al. Cell. 2012 Aug 31;150(5):934-47. PMID: 22939621 Mutagen: Bombardment Outcrossed: x2 Made by: Benjamin D. Towbin Received: 05/14/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW597 Species: C. elegans Genotype: gwIs39 III; dpy-13(eI84) ama-1(m118m251) IV; gwIs58. Description: gwIs39 [baf-1::gfp-lacI::let-858 3'UTR + vit-5::GFP] III. gwIs58 [hsp-16.2p::mCherry::256xLacO::4xLexA + unc-119(+)]. Small transgene/large array. Slow growing and dumpy. Expresses GFP-LacI from early embryogenesis and throughout development, which forms a small spot at the lacO array. Worms have green intestine (from late L4 stage). Might still carry unc-119(ed3) in background. Reference: Rohner S, et al. J Cell Biol. 2013 Mar 4;200(5):589-604. doi: 10.1083/jcb.201207024. PMID: 23460676 Mutagen: Outcrossed: x Made by: Sabine Rohner Received: 06/11/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW637 Species: C. elegans Genotype: met-2(n4256) set-25(n5021) III; gwIs4 X. Description: gwIs4 [myo-3p::RFP + baf-1::GFP-lacI:::let-858 3'UTR] X. Worms are slow growing, with reduced brood size and become sterile at elevated temperatures. met-2 and set-25 mutants co-segregate with ~ 20cM distance. Expresses GFP-LacI from early embryogenesis and throughout development, which forms a small spot at the lacO array. RFP expression in muscles. Reference: Towbin BD, et al. Cell. 2012 Aug 31;150(5):934-47. PMID: 22939621 Mutagen: Outcrossed: x Made by: Benjamin Towbin Received: 06/11/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW638 Species: C. elegans Genotype: met-2(n4256) set-25(n5021) III. Description: Worms are slow growing with reduced brood size and become sterile at elevated temperatures. Mutants co-segregate with ~ 20cM distance. Reference: Towbin BD, et al. Cell. 2012 Aug 31;150(5):934-47. PMID: 22939621 Mutagen: Outcrossed: x4 Made by: Benjamin D. Towbin Received: 05/14/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW76 Species: C. elegans Genotype: gwIs4 X. Description: gwIs4 [myo-3p::RFP + baf-1::GFP-lacI:::let-858 3'UTR] X. [NOTE: transgene seems prone to silencing. Pick RFP+ to maintain.] Superficially wild-type. Expresses GFP-LacI from early embryogenesis and throughout development, which forms a small spot at the lacO array. RFP expression in muscles. Reference: Meister P, et al. Genes Dev. 2010 Apr 15;24(8):766-82. PMID: 20395364 Mutagen: X-ray Outcrossed: x5 Made by: Peter Meister Received: 06/11/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW829 Species: C. elegans Genotype: gwIs39 III; cec-4(ok3124) IV; gwIs4 X. Description: gwIs39 [baf-1::gfp-lacI::let-858 3'UTR + vit-5::GFP] III. gwIs4 [baf-1p::GFP-lacI::let-858 3’UTR + myo-3p::RFP] X. Superficially wild-type. Expresses GFP-LacI throughout development from early embryogenesis, forming a large spot at the lacO array. Worms have red muscle (from L1 stage) and green intestine (from late L4 stage). Reference: Gonzalez-Sandoval A, et al. Cell. 2015 Dec 3;163(6):1333-47. PMID: 26607792 Mutagen: Outcrossed: x5 Made by: Adriana Gonzalez-Sandoval Received: 05/14/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW833 Species: C. elegans Genotype: cec-4(ok3124) IV; gwIs4 X. Description: gwIs4 [myo-3p::RFP + baf-1::GFP-lacI:::let-858 3'UTR] X. Superficially wild-type. Expresses GFP-LacI from early embryogenesis and throughout development, which forms a small spot at the lacO array. Worms have red muscle (from L1 stage). Reference: Gonzalez-Sandoval A, et al. Cell. 2015 Dec 3;163(6):1333-47. doi: 10.1016/j.cell.2015.10.066. PMID: 26607792. Mutagen: Outcrossed: xx5 Made by: Adriana Gonzalez Received: 06/11/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GW996 Species: C. elegans Genotype: gwSi17 set-4(n4600) II; met-2(n4256) set-25(n5021) III; gwIs4 X. Description: gwSi17 [cec‐4p::cec‐4::WmCherry::cec‐4 3'UTR] II. gwIs4 [baf-1p::GFP-lacI::let-858 3’UTR + myo-3p::RFP] X. Worms are slow growing with reduced brood size and become sterile at elevated temperatures. Expresses GFP-LacI throughout development from early embryogenesis, forming a large spot at the lacO array. Worms have red muscle (from L1 stage). CEC-4::WmCherry is visible at the nuclear periphery in embryos and L1 stage animals. Reference: Cabianca DS, et al. Nature 2019 May;569(7758):734-739. PMID: 31118512 Mutagen: MosSCI Outcrossed: x4 Made by: Adriana Gonzalez-Sandoval Received: 05/14/21 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: GWJ1 Species: Caenorhabditis elegans Genotype: lap-1(pk486). Description: 1063 bp deletion in gene from nucleotide 489 (intron 1) to 1552 (exon 3). Phenotype: slow growth. Mutagen: Outcrossed: x6 Made by: GWP Joshua Received: 09/10/01 Children's Hospital ----------------------------------------------------------------------------- Strain: GXW1 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated from soil under a Kiwi fruit tree in a botanical garden in Wuhan City, Hubei Province, China, on Nov 11, 2010. Lat: 30° 32' 34.40" N; Lon: 114° 25' 11.38" E. This strain was whole-genome sequenced as part of the Million Mutation Project (MMP). Mutagen: Outcrossed: x Made by: A Cutter & E Dolgin Received: 06/17/13 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: GZ264 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; isIs17. Description: isIs17 [pGZ295 (pie-1p::GFP::pcn-1(W03D2.4)) + pDP#MM051 (unc-119(+))] Maintain at 25C if possible to avoid possible germ line silencing. The entire coding sequence of PCNA (PCN-1, W03D2.4) was PCR-amplified and the resulting fragment cloned into pie-1::GFP germline expression vector to generate pGZ295, which was co-bombarded with pDP#MM051, which carries an unc-119 cDNA. The transgenic line behaves as though the two plasmids have been jointly integrated. Mutagen: Outcrossed: x Made by: Michael Brauchle Received: 05/15/08 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: GZ491 Species: Caenorhabditis elegans Genotype: dpy-11(e224) sas-5(t2033) V/nT1 [let-?(m435)] (IV;V). Description: Mutagen: EMS Outcrossed: x3 Made by: Marie de Lattre Received: 02/28/06 Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland ----------------------------------------------------------------------------- Strain: HA1019 Species: Caenorhabditis elegans Genotype: osm-11(rt142) X. Description: Isolated from a deletion library. Mutagen: Outcrossed: x6 Made by: Received: 08/01/08 Brown University ----------------------------------------------------------------------------- Strain: HA1706 Species: Caenorhabditis elegans Genotype: pha-1(e2123) III; rtEx726. Description: rtEx726 [del-1p::YC2.60 + pha-1(+)] expresses yellow cameleon YC2.60 in a subset of motor neurons. Maintain at 25C to select for the presence of the array. Haspel G, et al. (2010) J. Neurosci. 30:11151-6. Mutagen: Outcrossed: x0 Made by: Gal Haspel Received: 09/08/10 Brown University ----------------------------------------------------------------------------- Strain: HA1722 Species: Caenorhabditis elegans Genotype: rtIs29. Description: rtIs29 [acr-5p::YC2.60 + pha-1(+)] expresses yellow cameleon YC2.60 in all VB and DB motoneurons and some head and tail neurons. Maintain at 25C to select for the presence of the array. Haspel G, et al. (2010) J. Neurosci. 30:11151-6. Mutagen: UV Outcrossed: x0 Made by: Gal Haspel Received: 09/08/10 Brown University ----------------------------------------------------------------------------- Strain: HA1857 Species: Caenorhabditis elegans Genotype: osm-7(tm2256) III. Description: Mutagen: Outcrossed: x4 Made by: Received: 04/30/09 Brown University ----------------------------------------------------------------------------- Strain: HA2031 Species: Caenorhabditis elegans Genotype: rtIs31 X. Description: rtIs31 [elt-2p::GFP + Posm-10::HtnQ150]. Mutagen: Outcrossed: x Made by: Received: 05/05/08 Brown University ----------------------------------------------------------------------------- Strain: HA2040 Species: C.elegans Genotype: sir-2.4(n5137) I; sir-2.1(ok434) IV; sir-2.2(n5136) X. Description: Superficially wild-type. Reference: Anderson E, et al.Mech Ageing Dev. 2016 Mar;154:30-42. Mutagen: Outcrossed: x4 Made by: Edward Anderson Received: 01/31/20 Brown University ----------------------------------------------------------------------------- Strain: HA2619 Species: C.elegans Genotype: sod-1(tm776) II; rtSi1 IV. Description: rtSi1 [sod-1p::sod-1(WT) + Cbr-unc-119(+)] (inserted into cxTi10882) IV. Superficially wild-type. HA2619 serves as a control strain for HA2464. Reference: Baskoylu SN, et al. PLoS Genet. 2018;14(10):e1007682. Mutagen: MosSCI Outcrossed: x4 Made by: Saba Baskoylu Received: 01/31/20 Brown University ----------------------------------------------------------------------------- Strain: HA2823 Species: C.elegans Genotype: smn-1(rt248) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); nuIs175 X. Description: nuIs175 [myo-2p::RFP + unc-129p::RFP::snb-1] X. smn-1 heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP rt248 homozygotes (larval arrest). Homozygous hT2 [bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. NOTE: myo-2p::RFP is not visible in this strain. rt248 is a 8 bp deletion in smn-1. [rt248: TTTTGATTAGC--------ATCCCAAAC] [wild-type: TTTTGATTAGCTCCGTATCATCCCAAAC] Reference: Dimitriadi M, et al. Proc Natl Acad Sci U S A. 2016 Jul 26;113(30):E4377-86. O'Hern PJ, et al. Elife. 2017 May 2;6. pii: e20752. Mutagen: Outcrossed: x0 Made by: Patrick O'Hern Received: 10/02/18 Brown University ----------------------------------------------------------------------------- Strain: HA2825 Species: C.elegans Genotype: smn-1(ok355) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); rtSi10 IV; nuIs175 X. Description: rtSi10 [smn-1p::smn-1 + Cbr-unc-119(+)] IV. nuIs175 [myo-2p::RFP + unc-129p::RFP::snb-1] X. rtSi10 transgene partially rescues smn-1(ok355): smn-1 homozygotes normally arrest as larvae, but somatic defects, including late larval lethality, are ameliorated by rtSi10. Sterility in smn-1(ok355) homozygotes is not rescued by rtSi10. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok355 homozygotes (sterile due to partial rescue by rtSi10). Homozygous hT2[bli-4 let-? qIs48] inviable. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: O'Hern PJ, et al. eLife 2017;6:e20752 doi: 10.7554/eLife.20752 Mutagen: Outcrossed: x0 Made by: Patrick O'hern Received: 01/31/20 Brown University ----------------------------------------------------------------------------- Strain: HA2845 Species: C.elegans Genotype: fust-1(rt255) II. Description: Superficially wild-type. This is the appropriate control strain for FUS disease models HA2846 and HA2847. This control strain contains presumably silent edits inserted by CRISPR editing while creating the FUS disease models in HA2846 and HA2847, and was back-crossed to remove the pha-1 allele used in strain construction. Reference: Baskoylu S, et al. bioRxiv 799932; doi: https://doi.org/10.1101/799932 Mutagen: Outcrossed: x4 Made by: Saba Baskoylu Received: 01/31/20 Brown University ----------------------------------------------------------------------------- Strain: HA2846 Species: C.elegans Genotype: fust-1(rt256[R446S]) II. Description: fust-1(rt256[R446S]) was created by CRISPR editing of arginine codon in C. elegans fust-1 to create FUS disease model for human mutation R524S. This strain also contains additional silent edits (also present in control strain HA2845), and was back-crossed to remove the pha-1 allele used in strain construction. Under normal culture conditions HA2846 animals are superficially wild-type; after stress latent defects are observed. Reference: Baskoylu S, et al. bioRxiv 799932; doi: https://doi.org/10.1101/799932 Mutagen: Outcrossed: x4 Made by: Saba Baskoylu Received: 01/31/20 Brown University ----------------------------------------------------------------------------- Strain: HA2847 Species: C.elegans Genotype: fust-1(rt257[P447L]) II. Description: fust-1(rt257[P447L]) was created by CRISPR editing of proline codon in C. elegans fust-1 to create FUS disease model for human mutation P525L. This strain also contains additional silent edits (also present in control strain HA2845), and was back-crossed to remove the pha-1 allele used in strain construction. Under normal culture conditions HA2847 animals are superficially wild-type; after stress latent defects are observed. Reference: Baskoylu S, et al. bioRxiv 799932; doi: https://doi.org/10.1101/799932 Mutagen: Outcrossed: x4 Made by: Saba Baskoylu Received: 01/31/20 Brown University ----------------------------------------------------------------------------- Strain: HA2986 Species: C. elegans Genotype: sod-1(rt448[sod-1WT C]) II. Description: Superficially wild-type at 25C. Can be maintained 15-25C. rt448 is wild-type control strain for HA3299 containing all the silent codon changes needed for CRISPR/Cas9 genome editing. Reference: Baskoylu SN, et al. PLoS Genet. 2018;14(10):e1007682. NOTE: A micropublication on these strains (PMID: 33474528) incorrectly described sod-1(rt448) as the G85R mutant version and sod-1(rt449) as the control. Additionally, the strains list in the paper also incorrectly describes rt449 as sod-1 [G85RC]. The correct descriptions are that rt448 is the wild-type control, rt449 is the sod-1 [G93AC] mutant version, and rt451 is the sod-1 [G85RC] mutant version.] Mutagen: Crispr/Cas9 Outcrossed: x4 Made by: Saba Baskoylu Received: 12/04/18 Brown University ----------------------------------------------------------------------------- Strain: HA2987 Species: C. elegans Genotype: sod-1(rt449[G93A C]) II. Description: sod-1(rt449[G93A C]) was created by CRISPR editing of the cognate glycine codon in C. elegans sod-1 to create a disease model for human mutation G93A. This strain also contains additional silent edits, and was back-crossed to remove the pha-1 allele used in strain construction. Under normal culture conditions HA2987 animals are superficially wild-type; after stress latent defects are observed. Reference: Baskoylu S, et al. bioRxiv 799932; doi: https://doi.org/10.1101/799932 [NOTE: A micropublication on these strains (PMID: 33474528) incorrectly described sod-1(rt448) as the G85R mutant version and sod-1(rt449) as the control. Additionally, the strains list in the paper also incorrectly describes rt449 as sod-1 [G85RC]. The correct descriptions are that rt448 is the wild-type control, rt449 is the sod-1 [G93AC] mutant version, and rt451 is the sod-1 [G85RC] mutant version.] Mutagen: Outcrossed: x4 Made by: Saba Baskoylu Received: 01/31/20 Brown University ----------------------------------------------------------------------------- Strain: HA3 Species: Caenorhabditis elegans Genotype: nuIs11. Description: nuIs11 [osm-10::GFP + lin-15(+)]. Array contains pool28; KP#57-59 (osm-10::GFP insert at Nrul site) and pJM24 [lin-15(+) rescues n765 at 20C]. GFP expressed in ASH, ASI, PHA and PHB after 3-fold. nuIs11 may be inserted on LG I. Mutagen: Outcrossed: x4 Made by: Ann Hart Received: 03/19/01 Brown University ----------------------------------------------------------------------------- Strain: HA300 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; rtEx223. Description: rtEx223 [nlp-6p::GFP + lin-15(+)]. Maintain at 20C or warmer. Pick GFP+ non-Muv to maintain. Reference: Nathoo AN, et al. Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):14000-5. Mutagen: Outcrossed: x Made by: Anne Hart Received: 03/03/16 Brown University ----------------------------------------------------------------------------- Strain: HA307 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; rtEx224. Description: rtEx224 [F18E9.2::GFP + lin-15(+)]. Maintain by picking non-Muv. Maintain at 20C. Mutagen: Outcrossed: x Made by: Received: 12/18/07 Brown University ----------------------------------------------------------------------------- Strain: HA328 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; rtEx233. Description: rtEx233 [nlp-11p::GFP + lin-15(+)]. Raise at 20C or higher and pick non-Muv to maintain array. Mutagen: Outcrossed: x Made by: Arif Nathoo Received: 09/01/04 Brown University ----------------------------------------------------------------------------- Strain: HA329 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; rtEx234. Description: rtEx234 [nlp-13p::GFP + lin-15(+)]. Raise at 20C or higher and pick non-Muv or GFP+ to maintain array. Mutagen: Outcrossed: x Made by: Received: 11/05/07 Brown University ----------------------------------------------------------------------------- Strain: HA3299 Species: C. elegans Genotype: sod-1(rt451[sod-1(G85R C)]) II. Description: Superficially wild-type with increased sensitivity to paraquat in multiple assays. Can be maintained 15-25C. rt451 is CRISPR/Cas9 engineered G85R missense mutation in endogenous sod-1 locus mimicking human ALS SOD1 model. Reference: Baskoylu SN, et al. PLoS Genet. 2018;14(10):e1007682. NOTE: A micropublication on these strains (PMID: 33474528) incorrectly described sod-1(rt448) as the G85R mutant version and sod-1(rt449) as the control. Additionally, the strains list in the paper also incorrectly describes rt449 as sod-1 [G85RC]. The correct descriptions are that rt448 is the wild-type control, rt449 is the sod-1 [G93AC] mutant version, and rt451 is the sod-1 [G85RC] mutant version.] Mutagen: Crispr/Cas9 Outcrossed: x4 Made by: Saba Baskoylu Received: 12/04/18 Brown University ----------------------------------------------------------------------------- Strain: HA341 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; rtEx235. Description: rtEx235 [nlp-3p::GFP + lin-15(+)]. Maintain at 20C or warmer. Pick GFP+ non-Muv to maintain. Reference: Nathoo AN, et al. Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):14000-5. Mutagen: Outcrossed: x Made by: Anne Hart Received: 03/03/16 Brown University ----------------------------------------------------------------------------- Strain: HA353 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; rtEx247. Description: rtEx247 [nlp-14p::GFP + lin-15(+)]. Raise at 20C or higher and pick non-Muv to maintain array. Mutagen: Outcrossed: x Made by: Arif Nathoo Received: 09/01/04 Brown University ----------------------------------------------------------------------------- Strain: HA357 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; rtEx251. Description: rtEx251 [nlp-15p::GFP + lin-15(+)]. Raise at 20C or higher and pick non-Muv to maintain array. Mutagen: Outcrossed: x Made by: Arif Nathoo Received: 09/01/04 Brown University ----------------------------------------------------------------------------- Strain: HA367 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; rtEx256. Description: rtEx256 [nlp-18p::GFP + lin-15(+)]. Raise at 20C or higher and pick non-Muv to maintain array. Mutagen: Outcrossed: x Made by: Received: 11/05/07 Brown University ----------------------------------------------------------------------------- Strain: HA3703 Species: C. elegans Genotype: tdp-1(tgx58) I. Description: Null allele. CRISPR-engineered deletion of the tdp-1 locus precisely eliminates all known tdp-1 exons and introns. Reference: Lins J, et al. Generation of a C. elegans tdp-1 null allele and humanized TARDBP containing human disease-variants. MicroPubl Biol. 2023 Jun 6;2023:10.17912/micropub.biology.000693. doi: 10.17912/micropub.biology.000693. PMID: 37351305. Mutagen: Outcrossed: x4 Made by: nVivo Biosystems/ Nemametrix and Hart lab Received: 01/11/24 Brown University ----------------------------------------------------------------------------- Strain: HA371 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; rtEx260. Description: rtEx260 [nlp-19p::GFP + lin-15(+)]. Raise at 20C or higher and pick non-Muv to maintain array. Mutagen: Outcrossed: x Made by: Received: 05/28/08 Brown University ----------------------------------------------------------------------------- Strain: HA444 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; rtEx330. Description: rtEx330 [nlp-21p::GFP + lin-15(+)]. Raise at 20C or higher and pick non-Muv to maintain array. Mutagen: Outcrossed: x Made by: Arif Nathoo Received: 09/01/04 Brown University ----------------------------------------------------------------------------- Strain: HA446 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; rtEx332. Description: rtEx332 [nlp-20p::GFP + lin-15(+)]. Pick non-Muv or GFP+ to maintain. Mutagen: Outcrossed: x Made by: Received: 11/05/07 Brown University ----------------------------------------------------------------------------- Strain: HA449 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; rtEx335. Description: rtEx335 [nlp-10p::GFP + lin-15(+)]. Maintain at 20C or warmer. Pick GFP+ non-Muv to maintain. Reference: Nathoo AN, et al. Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):14000-5. Mutagen: Outcrossed: x Made by: Anne Hart Received: 03/03/16 Brown University ----------------------------------------------------------------------------- Strain: HA450 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; rtEx336. Description: rtEx336 [nlp-12p::GFP + lin-15(+)]. Raise at 20C or higher and pick non-Muv to maintain array. Mutagen: Outcrossed: x Made by: A Hart & A Nathoo Received: 06/02/04 Brown University ----------------------------------------------------------------------------- Strain: HA659 Species: Caenorhabditis elegans Genotype: rtIs11 V. Description: rtIs11 [osm-10p::GFP + osm-10p::HtnQ150 + dpy-20(+)]. HtnQ150 causes ASH degeneration 30% at 8 days. Mutagen: Outcrossed: x>1 Made by: DC King Received: 10/15/07 Brown University ----------------------------------------------------------------------------- Strain: HA661 Species: Caenorhabditis elegans Genotype: rtIs18 I. Description: rtIs18 [elt-2p::GFP + osm-10p::HtnQ150]. Mutagen: Outcrossed: x>1 Made by: DC King Received: 10/15/07 Brown University ----------------------------------------------------------------------------- Strain: HA759 Species: Caenorhabditis elegans Genotype: pqe-1(rt13) III; rtIs11 V. Description: rtIs11 [osm-10p::GFP + osm-10p::HtnQ150 + dpy-20(+)]. osm-10 promoter drives expression of both GFP and Htn-Q150 strongly in ASH and more weakly in other neurons of the head and tail. pqe-1(rt13) accelerates Htn-Q150 induced toxicity resulting in ASH neuron cell death predominantly during larval stages. Hence, many adult animals will lack overt GFP expression in ASH neurons. rtEx377 in the original HA759 was selected against leaving only rtIs11 in the strain available at the CGC. Mutagen: Outcrossed: x Made by: Peter Faber Received: 10/15/07 Brown University ----------------------------------------------------------------------------- Strain: HA865 Species: Caenorhabditis elegans Genotype: grk-2(rt97) III. Description: Grossly normal but defective for chemosensory response, including detection of octanol, diacetyl, and isoamyl alcohol. Mutagen: Outcrossed: x4 Made by: H Fukuto Received: 04/22/04 Brown University ----------------------------------------------------------------------------- Strain: HA98 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; rtEx66. Description: rtEx66 [nlp-2p::GFP + lin-15(+)]. Maintain at 20C or warmer. Pick GFP+ non-Muv to maintain. Reference: Nathoo AN, et al. Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):14000-5. Mutagen: Outcrossed: x Made by: Anne Hart Received: 03/03/16 Brown University ----------------------------------------------------------------------------- Strain: HAL100 Species: C. elegans Genotype: xpf-1(emc57[xpf-1::GFP]) II. Description: GFP tag inserted into endogenous xpf-1 locus. Ubiquitous GFP expression. Reference: Sabatella M, et al. Cell Rep. 2021 Jan 12;34(2):108608. PMID: 33440146 Mutagen: Outcrossed: x0 Made by: Karen Thijssen Received: 04/21/22 Erasmus University Medical Center, Rotterdam, The Netherlands ----------------------------------------------------------------------------- Strain: HAL227 Species: C. elegans Genotype: unc-119(ed3) III; emcSi70 IV. Description: emcSi70 [unc-119p::TIR1::mRuby] (IV: -0.05). TIR1 expression driven by the unc-119 promoter, allowing degradation of AID-tagged proteins specifically in neurons. Reference: Sabatella M, et al. Cell Rep. 2021 Jan 12;34(2):108608. PMID: 33440146 Mutagen: Outcrossed: x0 Made by: Karen Thijssen Received: 03/01/21 Erasmus University Medical Center, Rotterdam, The Netherlands ----------------------------------------------------------------------------- Strain: HAL230 Species: C. elegans Genotype: unc-119(ed3) III; emcSi71 IV. Description: emcSi71 [myo-3p::TIR1::mRuby] (IV: -0.05). TIR1 expression driven by the myo-3 promoter, allowing degradation of AID-tagged proteins specifically in muscles. Reference: Sabatella M, et al. Cell Rep. 2021 Jan 12;34(2):108608. PMID: 33440146 Mutagen: Outcrossed: x0 Made by: Karen Thijssen Received: 03/01/21 Erasmus University Medical Center, Rotterdam, The Netherlands ----------------------------------------------------------------------------- Strain: HAL240 Species: C. elegans Genotype: gtf-2H5(emcSi73[gtf-2H5-1::AID::GFP]) III. Description: CRISPR/Cas9-engineered insertion of AID::GFP tags into C-terminus of endogenous gtf-2H5 locus allowing auxin-induced degradation. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Karen Thijssen Received: 02/03/23 Erasmus University Medical Center, Rotterdam, The Netherlands ----------------------------------------------------------------------------- Strain: HAL504 Species: C. elegans Genotype: gtf-2H1(emcSi202[AID::GFP::gtf-2H1]) IV. Description: CRISPR/Cas9-engineered insertion of AID::GFP tags into N-terminus of endogenous gtf-2H1 locus allowing auxin-induced degradation. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Karen Thijssen Received: 02/03/23 Erasmus University Medical Center, Rotterdam, The Netherlands ----------------------------------------------------------------------------- Strain: HAL94 Species: C. elegans Genotype: gtf-2H5(tm6360) III. Description: Deletion allele generated by the Mitani Lab. Mutagen: TMP/UV Outcrossed: x4 Made by: Karen Thijssen Received: 02/03/23 Erasmus University Medical Center, Rotterdam, The Netherlands ----------------------------------------------------------------------------- Strain: HB101 Species: Escherichia coli Genotype: E. coli [supE44 hsdS20(rB-mB-) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1]. Description: Bacteria. This strain of E. coli is easier for worms to eat than other E. coli strains. [supE44 hsdS20(rB-mB-) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1]. Contains a mutation (rpsL20) in a ribosomal subunit gene that confers streptomycin resistance. Biosafety Level: BSL-1. Mutagen: Outcrossed: x Made by: Received: 08/18/97 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: HBR1000 Species: Caenorhabditis elegans Genotype: ceh-24(tm1103) V. Description: Flipping defective. Reference: Schwarz J & Bringmann H. eLife 2017;10.7554/eLife.24846. Mutagen: Outcrossed: x10 Made by: Juliane Schwarz Received: 04/03/17 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR1021 Species: Caenorhabditis elegans Genotype: goeIs240. Description: goeIs240 [hsp-16.2p::flp-11::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Likely intergrated into LG I or LG III. Heat shock-induced over-expression of FLP-11 neuropeptide causes behavioral quiescence. Reference: Turek et al. eLife 2016;5:e12499. Mutagen: Outcrossed: x2 Made by: Michal Turek Received: 03/31/16 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR1077 Species: Caenorhabditis elegans Genotype: goeIs247. Description: goeIs247 [ceh-24p::GCaMP6s::mKate2::unc-54 3'UTR + unc-119(+)]. Reporter expresses calcium sensor GCaMP6s with expression control mKate2. Reference: Schwarz J & Bringmann H. eLife 2017;10.7554/eLife.24846. Mutagen: Outcrossed: x2 Made by: Juliane Schwarz Received: 04/03/17 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR1110 Species: C. elegans Genotype: unc-119(ed3) III; goeIs257. Description: goeIs257 [nas- 38p::d1mGFP::nas-38 3'UTR + unc-119(+)]. Destabilized GFP expressed from the nas-38 promoter; especially visible in hypodermis and excretory system. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791 Mutagen: Microparticle Bombardment Outcrossed: x2 Made by: Florentin Masurat Received: 03/22/21 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR1261 Species: Caenorhabditis elegans Genotype: goeIs288. Description: goeIs288 [flp-11p::mKate2::unc-54 3'UTR + unc-119(+)]. Low copy number insertion. Integration site unknown, but likely not in LG II. Reference: Turek et al. eLife 2016;5:e12499. Mutagen: Outcrossed: x Made by: Received: 03/31/16 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR1549 Species: C. elegans Genotype: goeIs326. Description: goeIs326 [hsp-16.2p::nlp-29::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Over-expression of nlp-29::SL2::mKate2 after heat shock causes increased quiescence in adults. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791 Mutagen: Microparticle Bombardment Outcrossed: x2 Made by: Florentin Masurat Received: 03/22/21 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR1896 Species: C. elegans Genotype: goeIs388. Description: goeIs388 [hsp-16.2p::cnc-1::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Over-expression of cnc-1::SL2::mKate2 after heat shock causes increased quiescence in adults. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791 Mutagen: Microparticle Bombardment Outcrossed: x2 Made by: Florentin Masurat Received: 03/22/21 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR1897 Species: C. elegans Genotype: goeIs397. Description: goeIs397 [hsp-16.2p::cnc-10::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Over-expression of cnc-10::SL2::mKate2 after heat shock. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791 Mutagen: Microparticle Bombardment Outcrossed: x2 Made by: Florentin Masurat Received: 03/22/21 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR1899 Species: C elegans Genotype: goeIs406. Description: goeIs406 [hsp-16.2p::nlp-31::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Over-expression of nlp-31::SL2::mKate2 after heat shock. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791 Mutagen: Microparticle Bombardment Outcrossed: x2 Made by: Florentin Masurat Received: 03/22/21 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR1900 Species: C. elegans Genotype: goeIs408. Description: goeIs408 [hsp-16.2p::nlp-27::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Over-expression of nlp-27::SL2::mKate2 after heat shock. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791 Mutagen: Microparticle Bombardment Outcrossed: x2 Made by: Florentin Masurat Received: 03/22/21 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR1901 Species: C. elegans Genotype: goeIs407. Description: goeIs407 [hsp-16.2p::cnc-2::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Over-expression of cnc-2::SL2::mKate2 after heat shock. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791 Mutagen: Outcrossed: x2 Made by: Florentin Masurat Received: 03/22/21 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR1902 Species: C. elegans Genotype: goeIs409. Description: goeIs409 [hsp-16.2p::nlp-32::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Over-expression of nlp-32::SL2::mKate2 after heat shock. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791 Mutagen: Microparticle Bombardment Outcrossed: x2 Made by: Florentin Masurat Received: 03/22/21 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR1907 Species: C. elegans Genotype: goeIs410. Description: goeIs410 [hsp-16.2p::cnc-6::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Over-expression of cnc-6::SL2::mKate2 after heat shock. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791 Mutagen: Microparticle Bombardment Outcrossed: x2 Made by: Florentin Masurat Received: 03/22/21 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR191 Species: Caenorhabditis elegans Genotype: goeIs5. Description: goeIs5 [nmr-1p::SL1::GCaMP3.35::SL2::unc-54 3'UTR + unc-119(+)]. Reporter allows visualization of several command interneurons. Reference: Schwarz J & Bringmann H. PLoS One. 2013 Sep 20;8(9):e75853. Mutagen: Outcrossed: x2 Made by: Henrik Bringmann Received: 03/15/25 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR1911 Species: C. elegans Genotype: goeIs411. Description: goeIs411 [hsp-16.2p::cnc-4::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Over-expression of cnc-4::SL2::mKate2 after heat shock. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791 Mutagen: Microparticle Bombardment Outcrossed: x2 Made by: Florentin Masurat Received: 03/22/21 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR1912 Species: C. elegans Genotype: goeIs412. Description: goeIs412 [hsp-16.2p::cnc-7::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Over-expression of cnc-7::SL2::mKate2 after heat shock. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791 Mutagen: Microparticle Bombardment Outcrossed: x2 Made by: Florentin Masurat Received: 03/22/21 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR1914 Species: C elegans Genotype: goeIs413. Description: goeIs413 [hsp-16.2p::cnc-9::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Over-expression of cnc-9::SL2::mKate2 after heat shock. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791 Mutagen: Microparticle Bombardment Outcrossed: x2 Made by: Florentin Masurat Received: 03/22/21 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR1961 Species: C. elegans Genotype: goeIs431. Description: goeIs431 [hsp-16.2p::nlp-25::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Over-expression of nlp-25::SL2::mKate2 after heat shock. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791 Mutagen: Microparticle Bombardment Outcrossed: x2 Made by: Florentin Masurat Received: 03/22/21 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR1971 Species: Caenorhabditis elegans Genotype: nlp-42(syb235) V. Description: Complete CRISPR/Cas-9 knock-out (2317bp deletion) of the gene nlp-42(Y80D3A.10). Two times backcrossed with N2. Homozygous. Superficial wild-type. Primers for crossing: Fwd: cgagacttttaaccccgtcg InFwd: aaagcccatgacttgctgaa Rev: gctcaggtggttagagggtt Wild-type bands: 580bp, 2652bp. Mutation band: 335bp. Mutagen: Crispr/Cas9 Outcrossed: x2x Made by: SunyBiotech Corperation Received: 12/14/17 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR2025 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; goeEx711. Description: goeEx711 [nlp-42p::mGFP::unc-54 3'UTR + unc-119(+)]. Pick non-Unc to maintain. Construct contains 2000 bp of nlp-42 promoter upstream of start. Expression pattern is variable between worms. Mutagen: Outcrossed: x0 Made by: Jan Konietzka Received: 12/14/17 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR205 Species: Caenorhabditis elegans Genotype: goeIs22. Description: goeIs22 [mec-4p::SL1::GCaMP3.35::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Reporter allows visualization of several mechanosensitive neurons. Reference: Schwarz J & Bringmann H. PLoS One. 2013 Sep 20;8(9):e75853. Mutagen: Outcrossed: x2 Made by: Henrik Bringmann Received: 03/15/25 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR2256 Species: Caenorhabditis elegans Genotype: goeEx737. Description: goeEx737 [flp-24p::SL1::GCaMP3.35-SL2::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. ALA-specific expression of GCaMP with an additional mKate marker for expression reference. Pick mKate+ to maintain. High transmission rate (>99%). Reference: Konietzka et al. 2019. Current Biology (accepted). Mutagen: Outcrossed: x0 Made by: Dr. Jan Konietzka Received: 02/28/20 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR227 Species: Caenorhabditis elegans Genotype: aptf-1(gk794) II. Description: Complete lack of behavioral quiescence during sleep. References: Turek et al. Current Biology, 2013, 23, 2215-2223. Turek et al. eLife 2016;5:e12499. Mutagen: Outcrossed: x10 Made by: Michal Turek Received: 03/31/16 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR2317 Species: C. elegans Genotype: nlp-8(syb762) IV. Description: syb762 is a 1076 bp deletion in nlp-8. Flanking sequences: taaaacccggaacc - acttcttgaacaactg Forward primer: TAAAAGCGGAGTAGCGTCCA Reverse primer: CAGATGGTCGGGTGATTTGA syb762 was generated in a mutant background and out-crossed to N2 to remove those background mutations. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791 Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Marina Sinner /SunyBiotech Received: 03/22/21 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR232 Species: Caenorhabditis elegans Genotype: aptf-1(tm3287) II. Description: Complete lack of behavioral quiescence during sleep. Reference: Turek et al. Current Biology, 2013, 23, 2215-2223. Mutagen: Outcrossed: x10 Made by: Michal Turek Received: 03/31/16 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR2340 Species: C elegans Genotype: flp-11(syb1445[flp-11::SL2::unc-58(L428F)::linker::mKate2]) X. Description: unc-58(L428F) was knocked into the endogenous locus of flp-11 to express a sodium channel in RIS that causes strong overactivation of RIS. Reference: Busack I & Bringmann H. PLOS Genetics 19(3): e1010665. https://doi.org/10.1371/journal.pgen.1010665. Mutagen: Outcrossed: x1 Made by: Bringmann lab Received: 04/05/23 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR4 Species: Caenorhabditis elegans Genotype: goeIs3. Description: goeIs3 [myo-3p::SL1::GCamP3.35::SL2::unc54 3'UTR + unc-119(+)]. Reporter expresses the calcium indicator GCaMP3 in all body wall muscles. Reference: Schwarz J, et al. Worm. 2012 Jan 1;1(1):12-4. Mutagen: Outcrossed: x2 Made by: Ines Lewandrowski Received: 01/20/16 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR507 Species: Caenorhabditis elegans Genotype: flp-11(tm2706) X. Description: 70% reduction of behavioral quiescence during sleep. Reference: Turek et al. eLife 2016;5:e12499. Mutagen: Outcrossed: x7 Made by: Ines Lewandrowski Received: 03/31/16 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR546 Species: Caenorhabditis elegans Genotype: goeIs102. Description: goeIs102 [aptf-1 5'UTR::ChR2::mKate2::aptf-1 3'UTR + unc-119(+)]. Superficially wild-type. This strain carries an optogenetic transgene that can be used to send worms to sleep immediately at any given time point. Reference: Turek M, et al. Curr Biol. 2013 Nov 18;23(22):2215-2223. Mutagen: Outcrossed: x2 Made by: Henrik Bringmann Received: 03/18/19 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR762 Species: Caenorhabditis elegans Genotype: C10C6.7(goe3) IV. Description: goe3 is a 25 bp deletion in the second exon causing a frame shift and presumptive molecular null allele. sgRNA target sequence: GTTATGGTGAGAAGGAAAGCtgg Reference: Turek et al. eLife 2016;5:e12499. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Lewandrowski & Turek Received: 03/31/16 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR764 Species: Caenorhabditis elegans Genotype: C10C6.7(goe5) IV. Description: goe5 is a 4 bp deletion in the second exon causing a frame shift and presumptive molecular null allele. sgRNA target sequence: GTTATGGTGAGAAGGAAAGCtgg Reference: Turek et al. eLife 2016;5:e12499. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Lewandrowski & Turek Received: 03/31/16 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR914 Species: Caenorhabditis elegans Genotype: lim-6(tm4836) X. Description: Complete lack of behavioral quiescence during sleep. Reference: Turek et al. eLife 2016;5:e12499. Mutagen: Outcrossed: x7 Made by: Jan-Philipp Spies Received: 03/31/16 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HBR986 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; goeEx361. Description: goeEx361 [ceh-24p::ReaChr::mKate2::unc-54 3'UTR + unc-119(+)]. Pick non-Unc to maintain. Reporter expresses ReaChr with expression control mKate2. Reference: Schwarz J & Bringmann H. eLife 2017;10.7554/eLife.24846. Mutagen: Outcrossed: x0 Made by: Juliane Schwarz Received: 04/03/17 Technische Universitat Dresden ----------------------------------------------------------------------------- Strain: HC114 Species: Caenorhabditis elegans Genotype: ccIs4251 I; qtIs3 III; mIs11 IV; sid-1(qt9) V. Description: ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. qtIs3 [myo-2p::GFP dsRNA hairpin]. mIs11 [myo-2p::GFP + pes-10p::GFP + gut-promoter::GFP]. GFP expression in 4-cell embryos, pharyngeal muscle and gut. Resistant to systemic RNAi by feeding and injection and endogenous hairpin expression. Mutagen: EMS Outcrossed: x0 Made by: C. Molodowitch Received: 07/23/07 Harvard University, Cambridge, MA ----------------------------------------------------------------------------- Strain: HC196 Species: Caenorhabditis elegans Genotype: sid-1(qt9) V. Description: Null allele. Systemic RNAi defective. Reference: Jose AM, et al. Proc Natl Acad Sci U S A. 106(7):2283-8. Mutagen: EMS Outcrossed: x2 Made by: M. Sutherlin Causey Received: 11/03/14 Harvard University, Cambridge, MA ----------------------------------------------------------------------------- Strain: HC271 Species: Caenorhabditis elegans Genotype: ccIs4251 I; qtIs3 sid-2(qt42) III; mIs11 IV. Description: ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. qtIs3 [myo-2p::GFP dsRNA hairpin]. mIs11 [myo-2p::GFP + pes-10p::GFP + gut-promoter::GFP]. GFP expression in 4-cell embryos, pharyngeal muscle and gut. Resistant to systemic RNAi by feeding only. Mutagen: EMS Outcrossed: x0 Made by: Marie Sutherlin Received: 07/23/07 Harvard University, Cambridge, MA ----------------------------------------------------------------------------- Strain: HC48 Species: Caenorhabditis elegans Genotype: tbb-2(qt1) III. Description: Temperature sensitive embryonic lethal mutation with defects in centration and rotation of the centrosome pronuclear complex in the first cell division. Maintain at 15C. Mutagen: EMS Outcrossed: x2 Made by: Amanda Wright Received: 08/09/04 Harvard University, Cambridge, MA ----------------------------------------------------------------------------- Strain: HC726 Species: Caenorhabditis elegans Genotype: sid-1(qt95) V. Description: Sid. Hypomorhic allele. Reference: Whangbo JS, et al. G3 (Bethesda). 2017 Oct 12 [Epub ahead of print]. pii: g3.300308.2017. doi: 10.1534/g3.117.300308. PMID: 29025917. Mutagen: EMS Outcrossed: x4 Made by: George Lu Received: 01/29/18 Harvard University, Cambridge, MA ----------------------------------------------------------------------------- Strain: HC75 Species: Caenorhabditis elegans Genotype: ccIs4251 I; sid-1(qt2) V. Description: ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. Resistant to systemic RNAi, but sensitive to autonomous RNAi. Mutagen: EMS Outcrossed: x3 Made by: Received: 05/02/02 Harvard University, Cambridge, MA ----------------------------------------------------------------------------- Strain: HC970 Species: C. elegans Genotype: sid-1(qt78) V. Description: Sid. Deletion allele. Reference: Whangbo JS, et al. G3 (Bethesda). 2017 Oct 12 [Epub ahead of print]. pii: g3.300308.2017. doi: 10.1534/g3.117.300308. PMID: 29025917. Mutagen: EMS Outcrossed: x3 Made by: Phil Shiu Received: 01/29/18 Harvard University, Cambridge, MA ----------------------------------------------------------------------------- Strain: HCC21 Species: Caenorhabditis elegans Genotype: hwSi3 II; unc-119(ed9) III. Description: hwSi3 [pie-1p::GFP::mex-3::pie-1 3'UTR + unc-119(+)] II. Worms are healthiest at 15C. Shift to 25C overnight to increase brightness of GFP. Mos1-mediated single-copy insertion (MosSCI) into EG4322. GFP-tagged transgene expressing full-length MEX-3 uniformly throughout oocytes and early embryos through the 4-cell stage. GFP::MEX-3 then mirrors endogenous MEX-3, disappearing from somatic cells by about the 12-cell stage. Also detected in P granules. GFP::MEX-3 can replace endogenous MEX-3. Reference: Huang NN & Hunter CP. Gene. 2015 Jan 10;554(2):160-73. Mutagen: MosSCI Outcrossed: x1 Made by: Nancy N. Huang Received: 06/02/16 The Colorado College, Colorado Springs, CO ----------------------------------------------------------------------------- Strain: HCC27 Species: Caenorhabditis elegans Genotype: hwSi8 II; unc-119(ed9) III. Description: hwSi8 [pie-1p::GFP::mex-3(601-1248)::pie-1 3'UTR + unc-119(+)] II. Worms are healthiest at 15C. Shift to 25C overnight to increase brightness of GFP. Mos1-mediated single-copy insertion (MosSCI) into EG4322. GFP-tagged transgene expressing a portion of MEX-3(601-1248) required for protein degradation. At about the 12-cell stage, soma-germline asymmetry is briefly visible before GFP::MEX-3(601-1248) becomes undetectable even in the germline blastomeres. Detected weakly on P granules only in the presence of endogenous MEX. Reference: Huang NN & Hunter CP. Gene. 2015 Jan 10;554(2):160-73. Mutagen: MosSCI Outcrossed: x1 Made by: Nancy N. Huang Received: 06/02/16 The Colorado College, Colorado Springs, CO ----------------------------------------------------------------------------- Strain: HCC31 Species: Caenorhabditis elegans Genotype: hwSi13 II; unc-119(ed9) III. Description: hwSi13 [pie-1p::GFP::mex-3(2-636)::pie-1 3'UTR + unc-119(+)] II. Worms are healthiest at 15C. Shift to 25C overnight to increase brightness of GFP. Mos1-mediated single-copy insertion (MosSCI) into EG4322. GFP-tagged transgene expressing truncated MEX-3 that is missing a portion required for protein degradation. GFP::MEX-3(2-636) is extraordinarily stable, persisting in somatic cells through the comma stage (~550 cells) at levels similar to those in 1- and 2-cell embryos. Also detected on P granules through hatching. Reference: Huang NN & Hunter CP. Gene. 2015 Jan 10;554(2):160-73. Mutagen: MosSCI Outcrossed: x1 Made by: Nancy N. Huang Received: 06/02/16 The Colorado College, Colorado Springs, CO ----------------------------------------------------------------------------- Strain: HCL67 Species: C. elegans Genotype: unc-119(ed3) III; uocIs1. Description: uocIs1 [eft-3p::Cas9(dpiRNA)::tbb-2 3' UTR + unc-119(+)]. Cas9 is optimized to remove all piRNA sites. Cas9 is expressed in the germline. Reference: Zhang D, et al. Science. 2018 Feb 2;359(6375):587-592. Mutagen: Outcrossed: x0 Made by: Donglei Zhang Received: 03/05/18 Huazhong University of Science and Technology ----------------------------------------------------------------------------- Strain: HE1006 Species: Caenorhabditis elegans Genotype: rol-6(su1006) II. Description: Dominant Roller. Mutagen: EMS Outcrossed: x3 Made by: Zengel/Epstein Received: 04/29/94 Baylor College of Medicine, Houston, TX ----------------------------------------------------------------------------- Strain: HE110 Species: Caenorhabditis elegans Genotype: smg-1(re1) I; unc-97(su110) X. Description: Variable phenotype-WT when relaxed. Paralyzed. Dave Reiner identified smg-1(re1) in this strain. Mutagen: EMS Outcrossed: x Made by: Received: 01/01/81 Baylor College of Medicine, Houston, TX ----------------------------------------------------------------------------- Strain: HE115 Species: Caenorhabditis elegans Genotype: unc-100(su115) I. Description: Slow. Slightly Dpy. Recessive. Grows fairly well. Body Muscle abnormal. Sarcomeres faint. Mutagen: EMS Outcrossed: x Made by: Zengel J Received: 02/01/82 Baylor College of Medicine, Houston, TX ----------------------------------------------------------------------------- Strain: HE130 Species: Caenorhabditis elegans Genotype: unc-98(su130) X. Description: Movement WT. Body muscle abnormal. Mutagen: EMS Outcrossed: x Made by: Zengel J Received: 01/01/81 Baylor College of Medicine, Houston, TX ----------------------------------------------------------------------------- Strain: HE142 Species: Caenorhabditis elegans Genotype: unc-27(su142) X. Description: Slow moving Unc. Semi-dominant. Body muscle abnormal. Slightly small. Mutagen: EMS Outcrossed: x Made by: Zengel J Received: 01/01/81 Baylor College of Medicine, Houston, TX ----------------------------------------------------------------------------- Strain: HE151 Species: Caenorhabditis elegans Genotype: unc-96(su151) X. Description: Movement WT. Body muscle abnormal. Mutagen: EMS Outcrossed: x Made by: Zengel J Received: 01/01/81 Baylor College of Medicine, Houston, TX ----------------------------------------------------------------------------- Strain: HE177 Species: Caenorhabditis elegans Genotype: unc-94(su177) I. Description: Slow moving Unc. Body muscle abnormal. Slightly small. Mutagen: EMS Outcrossed: x Made by: Zengel J Received: 01/01/81 Baylor College of Medicine, Houston, TX ----------------------------------------------------------------------------- Strain: HE195 Species: Caenorhabditis elegans Genotype: unc-27(su195) X. Description: Semi-paralyzed Unc. Semi-dominant. Body muscle abnormal. Paracrystals. Mutagen: EMS Outcrossed: x Made by: Zengel J Received: 02/01/82 Baylor College of Medicine, Houston, TX ----------------------------------------------------------------------------- Strain: HE200 Species: Caenorhabditis elegans Genotype: unc-52(su200) II. Description: Mutagen: EMS Outcrossed: x Made by: Received: 01/01/81 Baylor College of Medicine, Houston, TX ----------------------------------------------------------------------------- Strain: HE2000 Species: Caenorhabditis elegans Genotype: unc-15(su2000) I. Description: Paralyzed Unc. Super Slow. Body Muscle Abnormal. Sarcomeres faint. Mutagen: UV Outcrossed: x Made by: Epstein H Received: 02/01/82 Baylor College of Medicine, Houston, TX ----------------------------------------------------------------------------- Strain: HE228 Species: Caenorhabditis elegans Genotype: unc-15(su228) I. Description: Unc. Body Muscle Abnormal. Recessive. Mutagen: EMS Outcrossed: x Made by: Zengel J Received: 02/01/82 Baylor College of Medicine, Houston, TX ----------------------------------------------------------------------------- Strain: HE250 Species: Caenorhabditis elegans Genotype: unc-52(e669su250) II. Description: Temperature sensitive Unc. Mutagen: EMS Outcrossed: x Made by: Received: 01/01/81 Baylor College of Medicine, Houston, TX ----------------------------------------------------------------------------- Strain: HE33 Species: Caenorhabditis elegans Genotype: unc-95(su33) I. Description: Unc-very slow or paralysed. Egl. Semi-dominant. Mutagen: EMS Outcrossed: x Made by: Received: 01/01/81 Baylor College of Medicine, Houston, TX ----------------------------------------------------------------------------- Strain: HE54 Species: Caenorhabditis elegans Genotype: unc-52(su54) II. Description: Mutagen: EMS Outcrossed: x Made by: Received: 01/01/81 Baylor College of Medicine, Houston, TX ----------------------------------------------------------------------------- Strain: HG231 Species: Caenorhabditis elegans Genotype: age-2(yw23) I. Description: HG231 exhibits an approximately 20% increase in mean, median and maxium lifespans compared to N2. HG231 exhibits somewhat reduced fertility and somewhat longer development time than N2. It has a normal appearance, movements and mating behavior. It's swimming rate in liquid is slightly higher than N2. STS mapping indicates the most likely location for age-2 is on LG I. Mutagen: EMS Outcrossed: x1 Made by: Yulong Yang Received: 05/04/98 University of Miami, Coral Gables, FL ----------------------------------------------------------------------------- Strain: HG284 Species: Caenorhabditis elegans Genotype: age-2(yw23) I; age-1(hx546) II. Description: This double mutant exhibits a doubling of both mean and maximum lifespans at 25C compared to N2. It exhibits a longer lifespan at 25C than it does at 20C. It has somewhat reduced fertility. It has normal development time, appearance, movements and feeding behavior. It's swimming rate in liquid is slightly higher than N2. STS mapping indicates the most likely location for age-2 is on LG I. Mutagen: Outcrossed: x Made by: Yulong Yang Received: 05/04/98 University of Miami, Coral Gables, FL ----------------------------------------------------------------------------- Strain: HGA8001 Species: Caenorhabditis elegans Genotype: lynEx1. Description: lynEx1 [nhr-80p::nhr-80::GFP + myo-2p::DsRed]. Pick animals with red pharynx to maintain. Lifespan is comparable to wild-type. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. Mutagen: Outcrossed: x0 Made by: Jerome Goudeau Received: 03/15/25 Ecole Normale Superieure, Lyon, France ----------------------------------------------------------------------------- Strain: HGA8002 Species: Caenorhabditis elegans Genotype: glp-1(e2141) III; lynEx1. Description: lynEx1 [(pJG01)nhr-80p::nhr-80::GFP + myo-2p::DsRed]. Sterile at 25C. Maintain at 20C or lower. Pick animals with red pharynx to maintain. Lifespan of animals carrying the array is longer than that of glp-1 alone. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. Mutagen: Outcrossed: x0 Made by: Jerome Goudeau Received: 03/15/25 Ecole Normale Superieure, Lyon, France ----------------------------------------------------------------------------- Strain: HGA8004 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; glp-1(e2141) III; lynEx1. Description: lynEx1 [(pJG01)nhr-80p::nhr-80::GFP + myo-2p::DsRed]. Sterile at 25C. Maintain at 20C or lower. Pick animals with red pharynx to maintain. Lifespan of animals carrying the array is longer than that of daf-16; glp-1 double mutants. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. Mutagen: Outcrossed: x0 Made by: Jerome Goudeau Received: 03/15/25 Ecole Normale Superieure, Lyon, France ----------------------------------------------------------------------------- Strain: HGA8005 Species: Caenorhabditis elegans Genotype: glp-1(e2141) III; daf-12(rh61rh411) X; lynEx1. Description: lynEx1 [(pJG01)nhr-80p::nhr-80::GFP + myo-2p::DsRed]. Sterile at 25C. Maintain at 20C or lower. Pick animals with red pharynx to maintain. Lifespan of animals carrying the array is longer than that of glp-1; daf-12 double mutants. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. Mutagen: Outcrossed: x0 Made by: Jerome Goudeau Received: 03/15/25 Ecole Normale Superieure, Lyon, France ----------------------------------------------------------------------------- Strain: HGA8006 Species: Caenorhabditis elegans Genotype: glp-1(e2141) III; fat-5 (tm420) V. Description: Sterile at 25C. Maintain at 20C or lower. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. Mutagen: Outcrossed: x0 Made by: Jerome Goudeau Received: 03/15/25 Ecole Normale Superieure, Lyon, France ----------------------------------------------------------------------------- Strain: HGA8007 Species: Caenorhabditis elegans Genotype: glp-1(e2141) III; fat-6 (tm331) IV. Description: Sterile at 25C. Maintain at 20C or lower. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. Mutagen: Outcrossed: x0 Made by: Jerome Goudeau Received: 03/15/25 Ecole Normale Superieure, Lyon, France ----------------------------------------------------------------------------- Strain: HGA8008 Species: Caenorhabditis elegans Genotype: glp-1(e2141) III; fat-7 (wa36) V. Description: Sterile at 25C. Maintain at 20C or lower. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. Mutagen: Outcrossed: x0 Made by: Jerome Goudeau Received: 03/15/25 Ecole Normale Superieure, Lyon, France ----------------------------------------------------------------------------- Strain: HGA8009 Species: Caenorhabditis elegans Genotype: glp-1(e2141) III; fat-5 (tm420) fat-7 (wa36) V. Description: Sterile at 25C. Maintain at 20C or lower. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. Mutagen: Outcrossed: x0 Made by: Jerome Goudeau Received: 03/15/25 Ecole Normale Superieure, Lyon, France ----------------------------------------------------------------------------- Strain: HGA8012 Species: Caenorhabditis elegans Genotype: glp-1(e2141) III; daf-9(rh50) X; lynEx1. Description: lynEx1 [nhr-80p::nhr-80::GFP + myo-2p::DsRed]. Sterile at 25C. Maintain at 15-20C. Temperature-sensitve Daf-c. Mig. Pick DsRed+ animals to maintain. Reference: Goudeau J, et al. PLoS Biol. 2011 Mar;9(3):e1000599. Mutagen: Outcrossed: x0 Made by: Jerome Goudeau Received: 03/15/25 Ecole Normale Superieure, Lyon, France ----------------------------------------------------------------------------- Strain: HH123 Species: Caenorhabditis elegans Genotype: unc-2(e55) unc-7(hs10) X. Description: unc-124 is a cold sensitive kinker Unc. Mutagen: EMS Outcrossed: x2 Made by: Jones/Hecht Received: 06/24/96 University of Houston, TX ----------------------------------------------------------------------------- Strain: HH132 Species: Caenorhabditis elegans Genotype: dpy-8(e130) unc-7(hs10) X. Description: Dpy. Cold sensitive kinker Unc. Mutagen: Outcrossed: x Made by: Received: 07/22/96 University of Houston, TX ----------------------------------------------------------------------------- Strain: HH142 Species: Caenorhabditis elegans Genotype: fer-1(b232) I. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x Made by: Received: 12/19/97 University of Houston, TX ----------------------------------------------------------------------------- Strain: HH16 Species: Caenorhabditis elegans Genotype: cdc-25.2(b262) unc-60(m35) V. Description: Unc. Temperature sensitive-maintain at 15C. Mutagen: Outcrossed: x Made by: Ralph Hecht Received: 12/16/96 University of Houston, TX ----------------------------------------------------------------------------- Strain: HH17 Species: Caenorhabditis elegans Genotype: unc-60(m35) cdc-25.2(b262) dpy-11(e224) V. Description: Dpy. Unc. Temperature sensitive-maintain at 15C. Mutagen: Outcrossed: x Made by: Ralph Hecht Received: 12/16/96 University of Houston, TX ----------------------------------------------------------------------------- Strain: HH19 Species: Caenorhabditis elegans Genotype: unc-1(hs1) X. Description: Cold sensitive Unc. Mutagen: EMS Outcrossed: x2 Made by: William Hones Received: 06/24/96 University of Houston, TX ----------------------------------------------------------------------------- Strain: HH27 Species: Caenorhabditis elegans Genotype: unc-127(hs13) V. Description: Cold sensitive coiler Unc. Mutagen: EMS Outcrossed: x2 Made by: William Jones Received: 06/24/96 University of Houston, TX ----------------------------------------------------------------------------- Strain: HH28 Species: Caenorhabditis elegans Genotype: unc-1(hs3) X. Description: Cold sensitive Unc. Mutagen: EMS Outcrossed: x2 Made by: William Jones Received: 06/24/96 University of Houston, TX ----------------------------------------------------------------------------- Strain: HH29 Species: Caenorhabditis elegans Genotype: unc-9(hs6) X. Description: Cold sensitive Unc. Mutagen: EMS Outcrossed: x2 Made by: William Jones Received: 06/24/96 University of Houston, TX ----------------------------------------------------------------------------- Strain: HH30 Species: Caenorhabditis elegans Genotype: unc-7(hs9) X. Description: Cold sensitive Unc. Mutagen: EMS Outcrossed: x2 Made by: William Jones Received: 06/24/96 University of Houston, TX ----------------------------------------------------------------------------- Strain: HH31 Species: Caenorhabditis elegans Genotype: unc-9(hs7) X. Description: Cold sensitive Unc. Mutagen: EMS Outcrossed: x2 Made by: William Jones Received: 06/24/96 University of Houston, TX ----------------------------------------------------------------------------- Strain: HH32 Species: Caenorhabditis elegans Genotype: unc-1(hs4) X. Description: Cold sensitive Unc. Mutagen: EMS Outcrossed: x2 Made by: William Jones Received: 06/24/96 University of Houston, TX ----------------------------------------------------------------------------- Strain: HH33 Species: Caenorhabditis elegans Genotype: unc-1(hs2) X. Description: Cold sensitive Unc. Mutagen: EMS Outcrossed: x2 Made by: William Jones Received: 06/24/96 University of Houston, TX ----------------------------------------------------------------------------- Strain: HH34 Species: Caenorhabditis elegans Genotype: unc-7(hs10) X. Description: Cold sensitive kinker Unc. Mutagen: EMS Outcrossed: x2 Made by: William Jones Received: 06/24/96 University of Houston, TX ----------------------------------------------------------------------------- Strain: HH35 Species: Caenorhabditis elegans Genotype: unc-9(hs8) X. Description: Cold sensitive Unc. Mutagen: EMS Outcrossed: x2 Made by: William Jones Received: 06/24/96 University of Houston, TX ----------------------------------------------------------------------------- Strain: HH52 Species: Caenorhabditis elegans Genotype: unc-126(hs12) III. Description: Cold sensitive paralyzed Unc. Mutagen: EMS Outcrossed: x2 Made by: William Jones Received: 06/24/96 University of Houston, TX ----------------------------------------------------------------------------- Strain: HH53 Species: Caenorhabditis elegans Genotype: unc-125(hs11) X. Description: Cold sensitive Unc. Mutagen: EMS Outcrossed: x2 Made by: William Jones Received: 06/24/96 University of Houston, TX ----------------------------------------------------------------------------- Strain: HH60 Species: Caenorhabditis elegans Genotype: unc-1(hs5) X. Description: Cold sensitive Unc. Mutagen: EMS Outcrossed: x2 Made by: William Jones Received: 06/24/96 University of Houston, TX ----------------------------------------------------------------------------- Strain: HJ1 Species: Caenorhabditis elegans Genotype: max-2(cy2) II; oxIs12 X. Description: oxIs12 [unc-47p::GFP + lin-15(+)]. Ventral cord commissural motor neuron axon guidance defects. Mild Unc. Mutagen: EMS Outcrossed: x>5 Made by: Mark Lucanic Received: 10/19/06 University of California, Davis, CA ----------------------------------------------------------------------------- Strain: HJ154 Species: Caenorhabditis elegans Genotype: max-2(nv162) II; oxIs12 X. Description: oxIs12 [unc-47p::GFP + lin-15(+)]. Ventral cord commissural motor neuron axon guidance defects. Mild Unc. Mutagen: UV/TMP Outcrossed: x>5 Made by: Mark Lucanic Received: 10/19/06 University of California, Davis, CA ----------------------------------------------------------------------------- Strain: HJZ102 Species: C. elegans Genotype: rike-1(syb1165) V/nT1[qIs51] (IV;V). Description: Heterozygotes are wild-type with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP rike-1(syb1165) homozygotes (early larval lethality). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Cheng X, et al. Autophagy. 2023 Jan;19(1):241-255. doi: 10.1080/15548627.2022.2071381. PMID: 35521960. Mutagen: Outcrossed: x2 Made by: SunyBiotech Received: 01/30/24 University of Macau ----------------------------------------------------------------------------- Strain: HK104 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: C. briggsae wild type strain collected from Okayama, Japan. Mutagen: Outcrossed: x Made by: H. Kagawa Received: 04/09/98 Wright State University ----------------------------------------------------------------------------- Strain: HK105 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: C. briggsae wild type strain collected in Sendai, Japan. Mutagen: Outcrossed: x Made by: H. Kagawa Received: 04/09/98 Wright State University ----------------------------------------------------------------------------- Strain: HK204 Species: Caenorhabditis elegans Genotype: khIs21. Description: khIs21 [(pRRZ4824) unc-68::GFP + rol-6(su1006)]. Rollers. Mutagen: UV Outcrossed: x1 Made by: Yasuji Sakube Received: 04/01/08 Okayama University, Okayama, Japan ----------------------------------------------------------------------------- Strain: HK205 Species: Caenorhabditis elegans Genotype: khIs22. Description: khIs22[(pRRG4824L) unc-68::GFP + rol-6(su1006)]. Rollers. Mutagen: UV Outcrossed: x2 Made by: Yasuji Sakube Received: 03/20/08 Okayama University, Okayama, Japan ----------------------------------------------------------------------------- Strain: HK30 Species: Caenorhabditis elegans Genotype: unc-68(kh30) V. Description: Mutagen: EMS Outcrossed: x>1 Made by: Hideki Ando Received: 06/08/94 Okayama University, Okayama, Japan ----------------------------------------------------------------------------- Strain: HK53 Species: Caenorhabditis elegans Genotype: unc-68(e540kh53) V. Description: Egl. Intragenic revertant. Mutagen: EMS Outcrossed: x0 Made by: Ryota Adachi Received: 04/01/08 Okayama University, Okayama, Japan ----------------------------------------------------------------------------- Strain: HL2000 Species: Caenorhabditis elegans Genotype: +/szT1 [lon-2(e678)] I; nhr-25(jm2389)/szT1 X. Description: Heterozygotes are WT and segregate WT, dead eggs, and Lon males. nhr-25 is homozygous embryonic lethal. Mutagen: UV/TMP Outcrossed: x Made by: Masako Asahina Received: 05/02/01 Institute of Parasitology, Branisovska, Czech Republic ----------------------------------------------------------------------------- Strain: HML1012 Species: Caenorhabditis elegans Genotype: cshIs140 II; ieSi58 IV. Description: cshIs140 [rps-28p::TIR1(F79G)::T2A::mCherry::his-11 + Cbr-unc-119(+)] II. ieSi58 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] IV. Ubiquitously expressed single copy, modified TIR1 allele, TIR1(F79G) that is compatible with 5-PH-IAA and can be used to deplete auxin-induced degradation-tagged (AID-tagged) proteins. Efficiently depletes target proteins at 1µM 5-Ph-IAA. Nuclear localized mCherry co-expression marker. Mutagen: Outcrossed: x4 Made by: Natalia Stec Received: 04/05/21 Cold Spring Harbor Laboratory ----------------------------------------------------------------------------- Strain: HML1035 Species: Caenorhabditis elegans Genotype: cshIs128 II; ieSi58 IV. Description: cshIs128 [rps-28p::TIR1::T2A::mCherry::his-11 + Cbr-unc-119(+)] II. ieSi58 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] IV. Companion strain to HML1012. Harbors conventional allele of TIR1 and Nuclear localized mCherry co-expression marker. Mutagen: Outcrossed: x4 Made by: Nat Received: 04/05/21 Cold Spring Harbor Laboratory ----------------------------------------------------------------------------- Strain: HMZ245 Species: C. elegans Genotype: ccar-1(sda11) IV. Description: Superficially wild-type except for a slightly shorter body length in adults. Crispr/Cas9 was used to create a 13 bp deletion in exon7 of ccar-1a; breakpoints: CTGATTCGGGAG/sda11/ATCGGAAGTTTC. sda11 is an isoform-specific deletion allele. It only affects the function of CCAR-1A and CCAR-1D, but not CCAR-1B and C. In addition, because CCAR-1D is not expressed in embryos,this allele can be used to specifically inactivate CCAR-1A (the full-length isoform that is the most similar to human CCAR1) during embryogenesis. Reference: Fu R, et al. J Cell Sci. 2018 May 10. Mutagen: Crispr/Cas9 Outcrossed: x4 Made by: Rong Fu Received: 06/11/18 IBMS, Soochow University, SuZhou, China ----------------------------------------------------------------------------- Strain: HP17 Species: Caenorhabditis elegans Genotype: sos-1(pd10) V. Description: sos-1 gain-of-function allele originally isolated as a suppressor of sem-5(n1619) lethality. pd10 causes a E99K substitution within the N-terminal histone fold domain of SOS-1. Single mutants appear wild-type. Mutagen: EMS Outcrossed: x1 Made by: Neil Hopper Received: 08/17/17 University of Pennsylvania, Philadelphia, PA ----------------------------------------------------------------------------- Strain: HP23 Species: Caenorhabditis elegans Genotype: sos-1(pd9) V. Description: sos-1(pd9) is a gain-of-function allele causing a C662Y substitution within the Ras exchange motif of SOS-1. Originally isolated as a suppressor of sem-5(n1619) lethality. Single mutants appear wild-type. Reference: Wooller A & Hopper N. (2014) European Worm Meeting. (Anyone using this allele may cite Neil Hopper as a personal communication based on this meeting abstract.) Mutagen: EMS Outcrossed: x0 Made by: Neil Hopper Received: 12/18/17 University of Pennsylvania, Philadelphia, PA ----------------------------------------------------------------------------- Strain: HR10 Species: Caenorhabditis elegans Genotype: dhc-1(ct42) dpy-5(e61)/unc-11(e47) bli-4(e937) I. Description: Heterozygtes are WT. Hets segregate WT, UncBli and DpyLet. ct42 homozygotes are inviable at all temperatures (recessive non-conditional larval lethal). ct42 is dominant temperature sensitive maternal effect lethal. ct42 hets give more inviable progeny at 25C than at 15C. dch-1 was previously called let-354(ct42). Mutagen: EMS Outcrossed: x14 Made by: Mains P Received: 01/29/91 University of Calgary, Calgary, Alberta ----------------------------------------------------------------------------- Strain: HR1060 Species: Caenorhabditis elegans Genotype: tbb-2(sb26) III. Description: Behaves as wild-type. Mutagen: EMS Outcrossed: x5 Made by: Paul Mains Received: 09/13/04 University of Calgary, Calgary, Alberta ----------------------------------------------------------------------------- Strain: HR1184 Species: Caenorhabditis elegans Genotype: +/szT1 [lon-2(e678)] I; nmy-1(sb115) dpy-8(e130)/szT1 X. Description: Heterozygotes are WT and segregate WT, Dpy Uncish with occasional Rol (nmy-1 dpy-8), Lon males, and dead eggs. nmy-1 homozygotes have a low brood size and are slow growing. sb115 is a null, truncation allele. Mutagen: EMS Outcrossed: x6 Made by: Paul Mains Received: 02/11/04 University of Calgary, Calgary, Alberta ----------------------------------------------------------------------------- Strain: HR1275 Species: Caenorhabditis elegans Genotype: src-1(cj293) dpy-5(e61)/hT2 [bli-4(e937) let-?(h661)] I; +/hT2 III. Description: Heterozygotes are WT and segregate WT, Dpy Mels and dead eggs. Mutagen: Outcrossed: x Made by: Paul Mains Received: 11/13/06 University of Calgary, Calgary, Alberta ----------------------------------------------------------------------------- Strain: HR1327 Species: Caenorhabditis elegans Genotype: mel-26(tm1664) unc-29(e1072) I. Description: Temperature sensitive. Maintain at 15C. Unc. Mutagen: Psoralen Outcrossed: x11 Made by: Paul Mains Received: 05/04/07 University of Calgary, Calgary, Alberta ----------------------------------------------------------------------------- Strain: HR16 Species: Caenorhabditis elegans Genotype: mel-23(ct45)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT and DpySteriles. ct45 homozygotes give only dead eggs at all temperatures. ct45 is ts dominant. Hets give more viable progeny at 15C than 25C. Mutagen: EMS Outcrossed: x13 Made by: Mains P Received: 10/14/91 University of Calgary, Calgary, Alberta ----------------------------------------------------------------------------- Strain: HR483 Species: Caenorhabditis elegans Genotype: mel-11(sb56) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUncs and Unc Steriles that are slightly Long. sb56 is a recessive zygotic suppressor of let-502(ca201) early larval lethality. Likely null of mel-11. Mutagen: EMS Outcrossed: x6 Made by: Wissmann/Mains Received: 09/13/99 University of Calgary, Calgary, Alberta ----------------------------------------------------------------------------- Strain: HR492 Species: Caenorhabditis elegans Genotype: +/hT2 I; vab-7(e1562) mel-44(sb44)/hT2 [bli-4(e937) let-?(h661)] III. Description: Dominant ts maternal-effect embryonic lethal. Embryos arrest prior to morphogenesis or at two-fold. Non-ts recessive mid-larval lethal. Maintain at 15C. Freezes poorly. Mutagen: EMS Outcrossed: x4 Made by: Paul Mains Received: 02/10/98 University of Calgary, Calgary, Alberta ----------------------------------------------------------------------------- Strain: HR505 Species: Caenorhabditis elegans Genotype: unc-59(e261) tba-2(sb51)/hIn1 [unc-54(h1040)] I. Description: Dominant temperature sensitive maternal-effect embryonic lethal. Maintain at 15C. Heterozygotes are WT. hIn1 homozygotes are Unc. Early cleavage spindles small and misoriented, cytokinesis often incomplete. Recessive non-temperature sensitive maternal-effect embryonic lethal (but sterile in conjuction with unc-59). Mutagen: EMS Outcrossed: x8 Made by: Paul Mains Received: 02/10/98 University of Calgary, Calgary, Alberta ----------------------------------------------------------------------------- Strain: HR592 Species: Caenorhabditis elegans Genotype: memi-1(sb41) dpy-20(e1282)/nT1 [unc-?(n754) let-?] IV; +/nT1 V. Description: Dominant ts maternal-effect embryonic lethal. Embryos show extensive cytoplasmic blebbing, often accompanied by small spindles and incomplete cytokinesis. Two cell embryos divide synchronously. Embryos arrest with eight to several hundred cells. Recessive non-ts maternal-effect embryonic lethal. Null may be WT. Maintain at 15C. Mutagen: EMS Outcrossed: x4 Made by: Paul Mains Received: 02/10/98 University of Calgary, Calgary, Alberta ----------------------------------------------------------------------------- Strain: HR596 Species: Caenorhabditis elegans Genotype: sbEx133. Description: sbEx133 [mel-11::GFP + rol-6(su1006)]. Maintain by picking Rollers. Mutagen: Outcrossed: x Made by: Andreas Wissmann Received: 03/07/07 University of Calgary, Calgary, Alberta ----------------------------------------------------------------------------- Strain: HR606 Species: Caenorhabditis elegans Genotype: sbEx136. Description: sbEx136 [(pAW33.1) let-502p::GFP + rol-6(su1006)]. pAW33.1 is about 6.6 kb BamH1 fragment from K06G1 and includes the first 12 amino acids of LET-502 clones into pPD95.67/BamH1. Should be nuclear localized, but is not nuclear localized. Maintain by picking Rollers. Mutagen: Outcrossed: x Made by: Andreas Wissmann Received: 03/07/07 University of Calgary, Calgary, Alberta ----------------------------------------------------------------------------- Strain: HR75 Species: Caenorhabditis elegans Genotype: mei-1(b284) unc-29(e1072)/mei-1(ct46) unc-13(e1091) I. Description: Heterozygotes are WT. ct46 is a dominant, temperature sensitive maternal effect lethal. ct46 homozygotes are viable and fertile at 15C. b284 is a non-conditional, recessive maternal effect lethal. Heterozygotes give more viable progeny at 15C than 25C. Mutagen: EMS Outcrossed: x10 Made by: Kemphues/Mains Received: 01/21/91 University of Calgary, Calgary, Alberta ----------------------------------------------------------------------------- Strain: HR767 Species: Caenorhabditis elegans Genotype: daf-8(e1393) mel-26(ct61sb4) I. Description: ct61 is a dominant, temperature sensitive, maternal effect lethal. sb4 is a revertant of the dominant phenotype. sb4 is a recessive, temperature sensitive, maternal effect lethal with approx. 10% hatching at 15C and 0% at 25C. Mutagen: EMS for sb4 Outcrossed: x7 Made by: Rhys Dow Received: 10/21/98 University of Calgary, Calgary, Alberta ----------------------------------------------------------------------------- Strain: HR83 Species: Caenorhabditis elegans Genotype: mel-24(ct59) dpy-20(e1282)/unc-24(e138) daf-15(m81) IV. Description: Heterozygotes are WT and segregate WT, Unc Dauers, and Dpys which throw dead eggs. Maintain by picking WT. ct59 animals are viable and fertile at 15C. ct59 is a temperature sensitive dominant maternal effect lethal. ct59 hets give more viable offspring at 15C than 25C. Mutagen: EMS Outcrossed: x12 Made by: Mains P Received: 01/29/91 University of Calgary, Calgary, Alberta ----------------------------------------------------------------------------- Strain: HR890 Species: Caenorhabditis elegans Genotype: +/szT1 [lon-2(e678)] I; syDf1/szT1 X. Description: Heterozygotes are Lon and segregate Lon and dead eggs. Mutagen: Outcrossed: x Made by: Received: 12/04/00 University of Calgary, Calgary, Alberta ----------------------------------------------------------------------------- Strain: HR899 Species: Caenorhabditis elegans Genotype: unc-101(m1) tba-2(sb25) I. Description: Unc. Mutagen: EMS Outcrossed: x5 Made by: Paul Mains Received: 09/13/04 University of Calgary, Calgary, Alberta ----------------------------------------------------------------------------- Strain: HR961 Species: Caenorhabditis elegans Genotype: ced-7(n1829) dpy-17(e164) III. Description: Dpy. Maternal effect persistent cell corpses. This was derived from MT4993; it was outcrossed once to get rid of an unlinked lethal. Hatching rate has improved from 10-70% to 98%. Mutagen: Outcrossed: x1 Made by: Paul Mains Received: 07/23/01 University of Calgary, Calgary, Alberta ----------------------------------------------------------------------------- Strain: HR973 Species: Caenorhabditis elegans Genotype: unc-101(m1) tba-2(sb27) I. Description: Unc. Mutagen: EMS Outcrossed: x5 Made by: Paul Mains Received: 09/13/04 University of Calgary, Calgary, Alberta ----------------------------------------------------------------------------- Strain: HR98 Species: Caenorhabditis elegans Genotype: mel-26(ct61) unc-29(e1072) I. Description: Dominant temperature sensitive maternal effect lethal. Will segregate some Vab. Maintain at 15C: about 30% of homozygotes hatch at 15C. Will not grow at 20C or 25C. Unc. Mutagen: EMS Outcrossed: x10 Made by: Received: 01/21/91 University of Calgary, Calgary, Alberta ----------------------------------------------------------------------------- Strain: HRN666 Species: C. elegans Genotype: wrt-10(aus36) II. Description: 5-bp deletion. Superficially wild-type. Reference: https://www.micropublication.org/journals/biology/micropub-biology-000169/ Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Tessa Sherry Received: 11/04/19 Monash University, Clayton, Australia ----------------------------------------------------------------------------- Strain: HRN667 Species: C. elegans Genotype: wrt-10(aus37) II. Description: 2-bp deletion. Superficially wild-type. Reference: https://www.micropublication.org/journals/biology/micropub-biology-000169/ Mutagen: CRISPER/CAS9 Outcrossed: x0 Made by: Tessa Sherry Received: 11/04/19 Monash University, Clayton, Australia ----------------------------------------------------------------------------- Strain: HRN680 Species: C. elegans Genotype: wrt-6(aus41) X. Description: 49-bp deletion. Superficially wild-type. Reference: https://www.micropublication.org/journals/biology/micropub-biology-000169/ Mutagen: Outcrossed: x0 Made by: Tessa Sherry Received: 11/04/19 Monash University, Clayton, Australia ----------------------------------------------------------------------------- Strain: HS1028 Species: Caenorhabditis elegans Genotype: mom-4(ne1539) I; lit-1(t1512) III. Description: This strain can grow at 11.5C but is embryonic lethal at 15C and above. Temperature shifts in late embryogenesis or in larval stages result in defects in many asymmetric cell divisions in postembryonic develoment. Mutagen: Outcrossed: x Made by: Received: 08/02/05 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS1204 Species: Caenorhabditis elegans Genotype: rmd-1&T05G5.9(tm1457) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are green and segregate green WT, dead eggs and nonGreens that lay dead eggs with the defects in spindle organization, chromosome segregation, and cytokinesis. Mutagen: UV/TMP Outcrossed: x Made by: S Mitani & K Oishi Received: 09/08/09 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS1215 Species: Caenorhabditis elegans Genotype: unc-76(e911) V; osEx211. Description: osEx211[apr-1::GFP + unc-76(+)]. This strain expresses functional APR-1::GFP driven by the apr-1 promoter. In the seam cells, just prior to the onset of mitosis, APR-1::GFP localizes to the anterior cortex. Mutagen: N/A Outcrossed: x Made by: Kota Mizumoto Received: 02/23/07 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS1222 Species: Caenorhabditis elegans Genotype: pbrm-1(tm415) I. Description: Sys. Low penetrance Psa. Reference: Shibata Y, et al. Dev Biol. 2012 Jan 15;361(2):349-57. Mutagen: EMS Outcrossed: x3 Made by: Yukimasa Shibata Received: 01/18/12 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS1257 Species: Caenorhabditis elegans Genotype: unc-76(e911) V; osEx219. Description: osEx219 [pbrm-1::GFP + unc-76(+)]. Pick wild-type to maintain. GFP expression in most somatic nuclei. Reference: Shibata Y, et al. Dev Biol. 2012 Jan 15;361(2):349-57. Mutagen: Outcrossed: x Made by: Yukimasa Shibata Received: 01/18/12 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS1294 Species: Caenorhabditis elegans Genotype: unc-76(e911) V; osEx225. Description: osEx225 [scm::dsh-2::venus + unc-76(+)]. Pick non-Unc to maintain. Reference: Mizumoto K, Sawa H. Dev Cell. 2007 Feb;12(2):287-99. Mutagen: Outcrossed: x0 Made by: Kota Mizumoto Received: 12/09/13 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS1325 Species: Caenorhabditis elegans Genotype: unc-76(e911) V; osEx229. Description: osEx229 [pry-1::GFP + unc-76(+)]. This strain expresses functional pry-1::GFP driven by the pry-1 promoter. In the seam cells, just prior to the onset of mitosis, pry-1::GFP localizes to the anterior cortex. Mutagen: Outcrossed: x Made by: Kota Mizumoto Received: 02/23/07 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS1337 Species: Caenorhabditis elegans Genotype: osIs1. Description: osIs1 [CYE-1::GFP (pMF101) + unc-76(+)]; probably integrated on LG II. This strain has Ste, Emb, Muv, Him phenotypes (probably dominant effects of the integration). Expression in many blast cells can be detected. Reference: Fujita et al. PLoS ONE 2, e407 (2007). Mutagen: Gamma ray Outcrossed: x1 Made by: Masaki Fujita Received: 02/05/10 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS1339 Species: Caenorhabditis elegans Genotype: osIs2. Description: osIs2 [CYE-1::GFP (pMF101) + unc-76(+)]; probably integrated on LG X. No dominant phenotypes observed (see HS1337). Expression in many blast cells can be detected, but much weaker than osIs1. Reference: Fujita et al. PLoS ONE 2, e407 (2007). Mutagen: Gamma ray Outcrossed: x3 Made by: Masaki Fujita Received: 02/05/10 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS1359 Species: Caenorhabditis elegans Genotype: unc-76(e911) V; osEx233. Description: osEx233 [scm::mig-5::venus + unc-76(+)]. Pick non-Unc to maintain. Reference: Mizumoto K, Sawa H. Dev Cell. 2007 Feb;12(2):287-99. Mutagen: Outcrossed: x0 Made by: Kota Mizumoto Received: 12/09/13 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS1380 Species: Caenorhabditis elegans Genotype: unc-76(e911) V; osEx240. Description: osEx240 [bet-1p::bet-1::GFP + unc-76(+)]. Pick Wild-type (non-Unc) to maintain. GFP expression in most somatic cells. Reference: Shibata Y, et al. Development. 2010 Apr;137(7):1045-53. Mutagen: Outcrossed: x Made by: Yukimasa Shibata Received: 10/10/11 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS1417 Species: Caenorhabditis elegans Genotype: osIs5 II. Description: osIs5 [scm::wrm-1::Venus + unc-76(+)]. WRM-1::GFP localizes to the anterior cortex in the seam cells prior to or during cell division, and to the posterior daughter's nucleus after cell division. Mutagen: Gamma Rays Outcrossed: x2 Made by: Hisako Takeshita Received: 02/23/07 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS143 Species: Caenorhabditis elegans Genotype: msi-1(os1) III. Description: 1.6 kb deletion in msi-1. Males have poor mating efficiency. Mutagen: UV/TMP Outcrossed: x10 Made by: Akinori Yoda Received: 10/25/00 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS144 Species: Caenorhabditis elegans Genotype: msi-1(os1) III; him-5(e1490) V. Description: 1.6 kb deletion in msi-1. Males have poor mating efficiency. Mutagen: UV/TMP Outcrossed: x10 Made by: Akinori Yoda Received: 10/25/00 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS1673 Species: Caenorhabditis elegans Genotype: lin-17(n3091) mom-5(ne12) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); vpIs1 X. Description: vpIs1 [elt-3::GFP + lin-15(+)] X. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP n3091 homozygotes (Sys Unc Psa). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Yamamoto Y, et al. PLoS Genet. 2011 Oct;7(10):e1002308. Mutagen: Outcrossed: x Made by: Hitoshi Sawa Received: 10/26/11 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS1675 Species: Caenorhabditis elegans Genotype: egl-20(n585) cwn-2(ok895) IV. Description: Egl. Unc. Reference: Yamamoto Y, et al. PLoS Genet. 2011 Oct;7(10):e1002308. Mutagen: Outcrossed: x Made by: Hitoshi Sawa Received: 10/26/11 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS1680 Species: Caenorhabditis elegans Genotype: lin-44(n1792) zdIs5 I; cwn-1(ok546) II; egl-20(n585) cwn-2(ok895) IV/nT1 [qIs51] (IV;V); mom-2(ne874) V/nT1. Description: zdIs5 [mec-4p::GFP + lin-15(+)] I. Homozygous nT1[qIs51] are inviable. mec-4::GFP is expressed in touch neurons. Heterozygotes are strong Egl Psa GFP+ and segregate dead eggs and non-GFP Unc Egl Psa that give only dead eggs at 25C. Mutagen: Outcrossed: x Made by: Hitoshi Sawa Received: 03/15/25 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS169 Species: Caenorhabditis elegans Genotype: nob-1(os6) III. Description: Tail abnormal. Defects in asymmetric T cell division causes Psa (phasmid socket absent) phenotype. Mutagen: EMS Outcrossed: x1 Made by: Hitoshi Sawa Received: 11/21/06 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS1698 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; osIs15. Description: osIs15 [pie-1p::GFP::apr-1 + unc-119(+)]. Non-Unc. Reference: Cell. 2011 Sep 16;146(6):942-54. Mutagen: Outcrossed: x Made by: Kota Mizumoto Received: 10/26/11 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS1749 Species: Caenorhabditis elegans Genotype: mig-1(e1787) lin-17(n671) mom-5(ne12) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Segregates WT GFP+ heterozygotes, non-GFP Unc Sys, very rare GFP+ homozygous hT2, and dead eggs. Maintain by picking wild-type GFP+. Reference: Yamamoto et al. PLoS Genet. 2011 Oct;7(10):e1002308. Mutagen: Outcrossed: x Made by: Hitoshi Sawa Received: 07/25/12 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS178 Species: Caenorhabditis elegans Genotype: psa-3(os8) X. Description: Superficially WT. Defects in asymmetric T cell division causes Psa (phasmid socket absent) phenotype. Mutagen: Outcrossed: x2 Made by: Received: 07/12/06 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS1790 Species: Caenorhabditis elegans Genotype: mig-1(e1787) lin-17(n671) mom-5(ne12) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); cfz-2(ok1201) V. Description: mig-1 confirmed by complementation tests, and cfz-2 by PCR. Segregates WT GFP+ heterozygotes, non-GFP Unc Sys, very rare GFP+ homozygous hT2, and dead eggs. Maintain by picking wild-type GFP+. Reference: Yamamoto et al. PLoS Genet. 2011 Oct;7(10):e1002308. Mutagen: Outcrossed: x Made by: Mayumi Shibuya Received: 07/25/12 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS1795 Species: Caenorhabditis elegans Genotype: dsh-2(or302) mig-5(tm2639)/mIn1 [dpy-10(e128) mIs14] II. Description: Heterozygotes are WT with pharyngeal GFP. Segregates WT GFP+, Dpy GFP+ (mIn1 homozygotes) and few GFP- dsh-2(or302) mig-5(tm2639) homozygotes (Sys). Pick WT GFP+ animals and check for correct segregation of progeny to maintain. Mutagen: Outcrossed: x Made by: Kota Mizumoto Received: 08/18/15 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS184 Species: Caenorhabditis elegans Genotype: swsn-4(os13) IV. Description: Egl, Pvul, Psa (Phasmid Socket Absent) and some embryonic lethality. The T cell division can be symmetric as in lin-17 mutants. Less severe at 15C. swsn-4 encodes a homolog of yeast SW12, a component of the SWI/SNF complex. Mutagen: EMS Outcrossed: x4 Made by: Hitoshi Sawa Received: 10/25/00 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS2067 Species: Caenorhabditis elegans Genotype: mig-1(e1787) lin-17(n671) mom-5(ne12) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); cfz-2(ok1201) wIs51 V; lin-18(e620) X. Description: wIs51 [SCMp::GFP + unc-119(+)] V. GFP expression in seam cells. Heterozygotes are GFP+(pharynx) wild-type and segregate GFP+(pharynx) wild-type, GFP-(pharynx) Sys Psa Unc and dead eggs. PIck GFP+(pharynx) wild-type to maintain. Presence of cfz-2 was confirmed by PCR; mig-1 by complementation test. Reference: Yamamoto Y, et al. PLoS Genet. 2011 Oct;7(10):e1002308. Mutagen: Outcrossed: x Made by: Hitoshi Sawa Received: 07/16/13 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS2326 Species: Caenorhabditis elegans Genotype: cwn-1(ok546) II; egl-20(n585) cwn-2(ok895) IV/nT1 [qIs51] (IV;V); vpIs1 X. Description: vpIs1 [elt-3::GFP + lin-15(+)] X. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok546 homozygotes (Unc Egl). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Yamamoto Y, et al. PLoS Genet. 2011 Oct;7(10):e1002308. Mutagen: Outcrossed: x Made by: Yuko Yamamoto Received: 10/26/11 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS2329 Species: Caenorhabditis elegans Genotype: unc-76(e911) V; osEx397. Description: osEx397 [cwn-1p::cwn-1::Venus + unc-76(+)]. Pick nonUnc to maintain. Transgene is expressed in tail region. Reference: Yamamoto et al. PLoS Genet. 2011 Oct;7(10):e1002308. Mutagen: Outcrossed: x Made by: Yuko Yamamoto Received: 01/05/12 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS2332 Species: Caenorhabditis elegans Genotype: unc-76(e911) V; osEx393. Description: osEx393 [cwn-2p::cwn-2::Venus + unc-76(+)]. Pick nonUnc to maintain. Transgene is expressed in the pharynx. Reference: Yamamoto et al. PLoS Genet. 2011 Oct;7(10):e1002308. Mutagen: Outcrossed: x Made by: Yuko Yamamoto Received: 01/05/12 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS2372 Species: Caenorhabditis elegans Genotype: mig-1(e1787) I; cfz-2(ok1201) wIs51 V; lin-18(e620) X. Description: wIs51 [SCMp::GFP + unc-119(+)] V. GFP expression in seam cells. Bivulva. Presence of cfz-2 was confirmed by PCR. mig-1 and lin-18 were confirmed by sequencing. Reference: Yamamoto Y, et al. PLoS Genet. 2011 Oct;7(10):e1002308. Mutagen: Outcrossed: x Made by: Yuko Yamamoto Received: 07/16/13 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS2690 Species: Caenorhabditis elegans Genotype: dsh-2(or302) dsh-1(ok1445)/mIn1 [dpy-10(e128) mIs14] II. Description: Heterozygotes are WT with pharyngeal GFP. Segregates WT GFP+, Dpy GFP+ (mIn1 homozygotes) and few GFP- dsh-2(or302) dsh-1(ok1445) homozygotes (Sys Unc). Pick WT GFP+ animals and check for correct segregation of progeny to maintain. Mutagen: Outcrossed: x Made by: Hitoshi Sawa Received: 08/18/15 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS2725 Species: Caenorhabditis elegans Genotype: dsh-2(or302) dsh-1(ok1445) mig-5(tm2639)/mIn1 [dpy-10(e128) mIs14] II. Description: Heterozygotes are WT with pharyngeal GFP. Segregates WT GFP+, Dpy GFP+ (mIn1 homozygotes) and non-GFP triple homozygotes (mostly Emb with a few animals surviving to early L1). Pick WT GFP+ animals and check for correct segregation of progeny to maintain. Mutagen: Outcrossed: x Made by: Hitoshi Sawa Received: 08/18/15 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS2728 Species: Caenorhabditis elegans Genotype: dsh-1(ok1445) mig-5(tm2639) II. Description: One or no gonad arm. Frequently ruptures at vulva. Mutagen: Outcrossed: x Made by: Hitoshi Sawa Received: 08/18/15 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS304 Species: Caenorhabditis elegans Genotype: swsn-1(os22) V. Description: Temperature sensitive. At 22.5C, maternal effect embryonic lethal. Temperature shift-up to 22.5C during embryogenesis results in animals with Egl, Pvul and Psa (phasmid socket absent) phenotypes. Shift-up to 25C results in growth arrest at larval stage. The T cell division can be symmetric as in lin-17 mutants. At 15C, nearly WT. Males grown at 15C can mate very well. psa-1 encodes a homolog of yeast SW13, a component of the SWI/SNF complex. Sequence data of this strain revealed the mutation is actually GTC/CCC/TCA to GTC/CTC/TCA causing a P86L substitution (G. Hayes). Mutagen: EMS Outcrossed: x4 Made by: Hitoshi Sawa Received: 10/25/00 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS321 Species: Caenorhabditis elegans Genotype: him-5(e1467) unc-76(e911) V; osEx67. Description: osEx67 [psa-4::GFP + unc-76(+)]. Maintain by picking non-Unc. Reference: Sawa H, et al. Mol Cell. 2000 Sep;6(3):617-24. Mutagen: Outcrossed: x Made by: Hitoshi Sawa Received: 11/17/10 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS325 Species: Caenorhabditis elegans Genotype: him-5(e1467) unc-76(e911) V; osEx71. Description: osEx71 [psa-1::GFP + unc-76(+)]. Maintain by picking non-Unc. Reference: Sawa H, et al. Mol Cell. 2000 Sep;6(3):617-24. Mutagen: Outcrossed: x Made by: Hitoshi Sawa Received: 11/17/10 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS3528 Species: Caenorhabditis elegans Genotype: osIs158 II. Description: osIs158 [eft-3p::AtTIR1(F79G)::mRuby] II. Single copy insertion into ttTi5605 on LG II. This strain expresses the improved version of TIR1 used for improved auxin-inducible degradation (AID2) technology. Reference: Negishi T, et al. Genetics. 2021 Dec 2;iyab218. doi: 10.1093/genetics/iyab218. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Takefumi Negishi Received: 01/25/22 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS3545 Species: C. elegans Genotype: osIs158 II; ieSi58 IV. Description: osIs158 [eft-3p::ccvTIR-1(F79G)::mRuby] single copy inserted into ttTi5605 on LG II. ieSi58 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] IV. This strain expresses the improved version of TIR1 used for improved auxin-inducible degradation (AID2) technology. Reference: Negishi T, et al. Genetics. 2021 Dec 2;iyab218. doi: 10.1093/genetics/iyab218. Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Takefumi Negishi Received: 11/30/20 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS3750 Species: Caenorhabditis elegans Genotype: ieSi58 IV; osIs182 V. Description: ieSi58 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] IV. osIs182 [eft-3p::AtTIR1(F79G) + LoxP + myo-2p::GFP + rps-27p::neoR + LoxP] V. ieSi58 is a single copy transgene inserted into chromosome IV (oxTi177) expressing degron::GFP in the soma. osIs182 is a single copy insertion of TIR1(F79G) into chromosome V (oxTi365) and expresses the improved version of TIR1 used for improved auxin-inducible degradation (AID2) technology. Reference: Negishi T, et al. Genetics. 2021 Dec 2;iyab218. doi: 10.1093/genetics/iyab218. Mutagen: Crispr/Cas9 Outcrossed: x1 Made by: Takefumi Negishi Received: 01/25/22 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS399 Species: Caenorhabditis elegans Genotype: let-526(os37) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT and GFP+. os37 homozygotes are Lvl and Psa. Pick GFP+ heterozygotes to maintain. qIs48 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter driving GFP in the intestine, and is homozygous lethal. Reference: Shibata Y, et al. Dev Biol. 2012 Jan 15;361(2):349-57. Mutagen: EMS Outcrossed: x3 Made by: Masahiro Uchida Received: 01/18/12 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS411 Species: Caenorhabditis elegans Genotype: ceh-20(os39) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT and GFP+. Segregate GFP- sterile Unc Psa (phasmid socket absent), very rare homozygous hT2 glowing animals, and dead eggs. ceh-20(os39) animals show defects in asymmetric T cell division. Mutagen: EMS Outcrossed: x1 Made by: Hitoshi Sawa Received: 11/21/06 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS428 Species: Caenorhabditis elegans Genotype: dpy-22(os26) X; osEx89. Description: osEx89 [col-10::GFP + dpy-22(+)]. Animals with the array are non-Dpy and GFP+. Animals which have lost the array are Dpy, Egl, and GFP-. Mutagen: Outcrossed: x3 Made by: Received: 08/02/05 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS445 Species: Caenorhabditis elegans Genotype: dpy-22(os38) X; osEx89. Description: osEx89 [col-10::GFP + dpy-22(+)]. Animals with the array are non-Dpy and GFP+. Animals which have lost the array are Dpy, Egl, and GFP-. Mutagen: Outcrossed: x3 Made by: Received: 08/02/05 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS458 Species: Caenorhabditis elegans Genotype: let-19(os33)/mIn1 [dpy-10(e128) mIs14] II. Description: Heterozygotes are WT GFP+ and segregate WT GFP+, Dpy GFP+ (mIn1 homozygotes), and os33 homozygotes (GFP-, Dpy, Muv, Steriles). Mutagen: Outcrossed: x10 Made by: Received: 08/02/05 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS616 Species: Caenorhabditis elegans Genotype: osEx108. Description: osEx108 [(pAY105) let-19::GFP + rol-6(su1006)]. Rollers. Pick rollers to maintain. Reference: Yoda et al. Development (2005) vol. 132 (8) pp. 1885-93. Mutagen: Outcrossed: x Made by: Hitoshi Sawa Received: 05/13/11 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS634 Species: Caenorhabditis elegans Genotype: rnt-1(os58) I. Description: Phasmid socket absent. Mutagen: Outcrossed: x1 Made by: Received: 11/15/05 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS661 Species: Caenorhabditis elegans Genotype: nob-1(os68) III. Description: Healthy. Abnormal morphology of the tail (only at Nomarski level). Defects in asymmetric T cell division causes Psa (phasmid socket absent). Mutagen: EMS Outcrossed: x0 Made by: Hitoshi Sawa Received: 11/21/06 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS732 Species: Caenorhabditis elegans Genotype: wrm-1(tm514) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP tm514 homozygotes (probable embryonic or early larval arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Pick WT GFP and check for correct segregation of progeny to maintain. Mutagen: Outcrossed: x4 Made by: Received: 08/02/05 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS845 Species: Caenorhabditis elegans Genotype: osEx138. Description: osEx138 [let-526::GFP + rol-6(su1006)]. Rollers. Pick rollers to maintain. GFP expression in most somatic nuclei. Reference: Shibata Y, et al. Dev Biol. 2012 Jan 15;361(2):349-57. Mutagen: Outcrossed: x Made by: Masahiro Uchida Received: 01/18/12 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS886 Species: Caenorhabditis elegans Genotype: bet-1(os46) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT and GFP in the pharynx, and segregate WT GFP+, Sterile Psa(Phasmid socket absent) non-GFP homozygous os46) animals and dead eggs. Maintain by picking GFP progeny. Reference: Shibata et al. Development in press (2010). Mutagen: EMS Outcrossed: x3 Made by: Yukimasa Shibata Received: 02/05/10 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HS942 Species: Caenorhabditis elegans Genotype: unc-76(e911) V; osEx158. Description: osEx158 [wrm-1::GFP + unc-76(+)]. Animals with the array are WT and GFP+. Animals which have lost the array are Unc and GFP-. Mutagen: Outcrossed: x Made by: Received: 08/02/05 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: HT1011 Species: Caenorhabditis elegans Genotype: lpIs100. Description: lpIs100 [tub-1::GFP + rol-6(su1006)]. Rollers. Reference: Mukhopadhyay A, et al. Cell Metab. 2005 Jul;2(1):35-42. Mutagen: Outcrossed: x Made by: A. Mukhopadhyay Received: 07/21/11 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1037 Species: Caenorhabditis elegans Genotype: lpIs101. Description: lpIs101 [tub-1::GFP + rol-6(su1006)]. Rollers. Reference: Mukhopadhyay A, et al. Cell Metab. 2005 Jul;2(1):35-42. Mutagen: Outcrossed: x Made by: A. Mukhopadhyay Received: 07/21/11 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT115(DE3) Species: Escherichia coli Genotype: E. coli [F-, mcrA, mcrB, IN(rrnD-rrnE)1, rnc14::Tn10(DE3 lysogen: lacUV5 promoter -T7 polymerase]. Description: Bacteria. Genotype: F-, mcrA, mcrB, IN(rrnD-rrnE)1, rnc14::Tn10(DE3 lysogen: lacUV5 promoter -T7 polymerase) (IPTG-inducible T7 polymerase) (RNAse III minus). This strain grows on LB or 2XYT plates. This strain is tetracycline resistant. Researchers using this strain should test for expression by transforming in one of the plasmids from the Fire Vector Kit (1999) (pLT76, e.g.) using standard CaCl2 transformation techniques. Biosafety Level: BSL-1. Mutagen: Outcrossed: x Made by: Lisa Timmons Received: 06/09/99 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: HT1361 Species: C. elegans Genotype: lpIs32. Description: lpIs32 [ftt-2::gfp + rol-6]. Rollers. FTT-2::GFP is expressed strongly in the pharynx, and can be observed in the neurons in the head, body and tail, and also weakly expressed in the intestine. Reference: Wang Y, et al. Mech Ageing Dev. 2006 Sep;127(9):741-7. PMID: 16860373. Mutagen: Outcrossed: x2 Made by: Yamei Wang Received: 09/23/22 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1593 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III. Description: Unc. Derived from DP38 by outcrossing four additional times to remove the Daf mutation present in DP38. Mutagen: EMS Outcrossed: x7 Made by: SD Narasimhan Received: 04/18/08 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1686 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx61. Description: wwEx61 [ins-1p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 06/07/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1687 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx62. Description: wwEx62 [ins-2p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 04/25/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1690 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwIs26. Description: wwIs26 [ins-3p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 04/25/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1693 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx63. Description: wwEx63 [ins-4p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 04/25/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1695 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx64. Description: wwEx64 [ins-5p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 04/25/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1701 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx65. Description: wwEx65 [ins-6p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 06/24/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1702 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx66. Description: wwEx66 [ins-7p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 04/25/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1704 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx67. Description: wwEx67 [ins-8p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 04/25/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1708 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx68. Description: wwEx68 [ins-9p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 04/25/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1711 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx69. Description: wwEx69 [ins-10p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 06/07/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1714 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx70. Description: wwEx70 [ins-11p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 06/07/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1718 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwIs27. Description: wwIs27 [ins-12p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 06/24/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1720 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwIs28. Description: wwIs28 [ins-13p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 04/25/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1722 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwIs29. Description: wwIs29 [ins-14p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 06/07/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1728 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx71. Description: wwEx71 [ins-15p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 04/25/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1730 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx72. Description: wwEx72 [ins-16p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 04/25/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1734 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx73. Description: wwEx73 [ins-17p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 04/25/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1737 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx74. Description: wwEx74 [ins-18p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 04/25/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1741 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwIs30. Description: wwIs30 [ins-19p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x1 Made by: Walhout/Tissenbaum Received: 03/15/25 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1743 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx75. Description: wwEx75 [ins-20p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 06/07/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1747 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwIs31. Description: wwIs31 [ins-21p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 06/24/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1753 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwIs32. Description: wwIs32 [ins-22p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 06/24/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1756 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwIs33. Description: wwIs33 [ins-23p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 04/25/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1757 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwIs34. Description: wwIs34 [ins-24p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 06/24/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1764 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx76. Description: wwEx76 [ins-25p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 06/07/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1765 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx77. Description: wwEx77 [ins-26p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 06/24/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1768 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwIs35. Description: wwIs35 [ins-27p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 04/25/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1771 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx78. Description: wwEx78 [ins-28p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 06/24/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1774 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwIs36. Description: wwIs36 [ins-29p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 08/21/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1780 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx79. Description: wwEx79 [ins-30p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 06/07/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1785 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx80. Description: wwEx80 [ins-32p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 06/07/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1788 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx81. Description: wwEx81 [ins-33p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 06/24/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1798 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwIs39. Description: wwIs39 [ins-35p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 04/25/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1803 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwIs40. Description: wwIs40 [ins-37p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 06/07/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1806 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx83. Description: wwEx83 [ins-38p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 06/07/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1809 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx84. Description: wwEx84 [ins-39p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 04/25/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1881 Species: Caenorhabditis elegans Genotype: daf-16(mgDf50) I; daf-2(e1370) unc-119(ed3) III; lpIs12. Description: lpIs12 [daf-16a::RFP + unc-119(+)]. Maintain at 15C. Extended lifespan. Reference: Kwon ES, et al., Nature. 2010 Jul 22;466(7305):498-502. Mutagen: Bombardment Outcrossed: x4 Made by: Eun-Soo Kwon Received: 02/01/11 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1882 Species: Caenorhabditis elegans Genotype: daf-16(mgDf50) I; daf-2(e1370) unc-119(ed3) III; lpIs13. Description: lpIs13 [daf-16b::CFP + unc-119(+)]. Maintain at 15C. Extended lifespan. Reference: Kwon ES, et al., Nature. 2010 Jul 22;466(7305):498-502. Mutagen: Bombardment Outcrossed: x4 Made by: Eun-Soo Kwon Received: 02/01/11 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1883 Species: Caenorhabditis elegans Genotype: daf-16(mgDf50) I; daf-2(e1370) unc-119(ed3) III; lpIs14. Description: lpIs14 [daf-16f::GFP + unc-119(+)]. Maintain at 15C. Extended lifespan. Reference: Kwon ES, et al., Nature. 2010 Jul 22;466(7305):498-502. Mutagen: Bombardment Outcrossed: x4 Made by: Eun-Soo Kwon Received: 02/01/11 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1888 Species: Caenorhabditis elegans Genotype: daf-16(mgDf50) I; unc-119(ed3) III; lpIs12. Description: lpIs12 [daf-16a::RFP + unc-119(+)]. Over-expresses daf-16a. Maintain under standard conditions. Reference: Kwon ES, et al. Nature. 2010 Jul 22;466(7305):498-502. Mutagen: Bombardment Outcrossed: x4 Made by: Eun-Soo Kwon Received: 07/21/11 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1889 Species: Caenorhabditis elegans Genotype: daf-16(mgDf50) I; unc-119(ed3) III; lpIs14. Description: lpIs14 [daf-16f::GFP + unc-119(+)]. Over-expresses daf-16f. Maintain under standard conditions. Reference: Kwon ES, et al. Nature. 2010 Jul 22;466(7305):498-502. Mutagen: Bombardment Outcrossed: x4 Made by: Eun-Soo Kwon Received: 07/21/11 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT1890 Species: Caenorhabditis elegans Genotype: daf-16(mgDf50) I; daf-2(e1370) III. Description: Short-lived. Dauer-defective (Daf-d). Maintain under standard conditions. Reference: Kwon ES, et al. Nature. 2010 Jul 22;466(7305):498-502. Mutagen: Outcrossed: x Made by: Eun-Soo Kwon Received: 07/21/11 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT2098 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx82. Description: wwEx82 [ins-36p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 06/24/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT2099 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; wwEx85. Description: wwEx85 [daf-28p::GFP + unc-119(+)]. Pick wild-type (non-Unc) to maintain. Reference: Ritter AD, et al. Genome Res. 2013 Mar 28. Mutagen: Bombardment Outcrossed: x0 Made by: Walhout/Tissenbaum Received: 06/24/13 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT941 Species: Caenorhabditis elegans Genotype: lpIs1. Description: lpIs1 [jnk-1(+) + rol-6(su1006)]. Rollers. Over-expresses jnk-1(+) with rol-6. Shows life span extension. Integration of lpEx1. Mutagen: Outcrossed: x2 Made by: Seung Wook Oh Received: 05/16/05 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HT942 Species: Caenorhabditis elegans Genotype: lpIs2. Description: lpIs2 [jnk-1(+) + rol-6(su1006)]. Over-expresses jnk-1(+) with rol-6. Shows life span extension. Integration of lpEx1. Rollers. Mutagen: Outcrossed: x2 Made by: Seung Wook Oh Received: 05/16/05 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: HW1329 Species: Caenorhabditis elegans Genotype: lin-41(xe11) I. Description: Egg-laying (Egl) defects and subsequent internal hatching of progeny (Bagging) in > 95% of animals. xe11 is a C-to-U point mutation in each of the endogenous let-7 complementary sites, LCS1 and LCS2 [I:C9,335,211T & I:C9,335,260T]. xe11 is a weak gain-of-function allele: mutation of two functionally relevant let-7 binding sites impairs repression by let-7 causing over-expression of LIN-41 in L4 stage animals. Reference: Ecsedi M, et al. Dev Cell. 2015 Feb 9;32(3):335-44. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Matyas Escedi Received: 09/14/16 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: HW1822 Species: Caenorhabditis elegans Genotype: lin-29(xe61[lin-29::gfp::3xflag]) II Description: Low penetrance Pvl (i.e., tagged protein appears not fully functional). CRISPR/Cas9-engineered allele adds GFP and 3xflag tag to LIN-29. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Reference: Aeschimann F, et al., Mol Cell. 2017 Feb 2;65(3):476-489. Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Florian Aeschimann Received: 02/05/19 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: HW1826 Species: Caenorhabditis elegans Genotype: lin-29(xe63[gfp::3xflag::lin-29a]) II. Description: Superficially wild-type. CRISPR/Cas9-engineered allele adds GFP and 3xflag tag to the N-terminus of LIN-29A. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Reference: Aeschimann F, et al., (2019). A single let-7 target to coordinate transition to adulthood. Life Science Alliance 2, e201900335. http://dx.doi.org/10.26508/lsa.201900335 ) Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Florian Aeschimann Received: 02/05/19 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: HW1835 Species: C. elegans Genotype: lin-29(xe40 xe65[lin-29b::gfp::3xflag]) II. Description: Partially penetrant Pvl and Egl. xe40 specifically disrupts the LIN-29A isoform. The GFP tag was inserted at the shared C-terminus of LIN-29A/B in the xe40 background, so only the labeled B isoform is expressed. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Reference: Pereira et al., (2019) Timing mechanism of sexually dimorphic nervous system differentiation. eLIFE 8: e42078. https://doi.org/10.7554/eLife.42078 Mutagen: CRISPR-Cas9 Outcrossed: x3 Made by: Florian Aeschimann Received: 02/05/19 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: HW1870 Species: Caenorhabditis elegans Genotype: lin-41(xe8)/lin-41(bch28[eft-3p::gfp::h2b::tbb-2 3'UTR] xe70) I. Description: Pick GFP+ Egl to maintain. Segregates GFP- lin-41(xe8) homozygotes (die by vulval bursting as young adults), lin-41(xe8)/lin-41(bch28[eft-3p::gfp::h2b::tbb-2 3'UTR] xe70) heterozygotes (GFP+ Egl), and lin-41(bch28[eft-3p::gfp::h2b::tbb-2 3'UTR] xe70) (GFP+ Ste Dpy). lin-41(xe8) is a deletion of let-7 binding sites in the lin-41 3'UTR. The balancer was derived from lin-41(bch28), a lin-41(0) allele in which an expression cassette that drives ubiquitous nuclear GFP from the eft-3 promoter has been inserted into the lin-41 coding sequence (Katic et al., G3 (2015) 5:1649-56). References: Katic et al., G3 (2015) 5:1649-56 for lin-41(bch28) starting allele for balancer generation. Aeschimann F, et al. (2019). A single let-7 target to coordinate transition to adulthood. Life Science Alliance 2, e201900335. for balanced line. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x3 Made by: Jun Liu, Iskra Katic Received: 02/05/19 Friedrich Miescher Institute, Basel, Switzerland ----------------------------------------------------------------------------- Strain: HX103 Species: Caenorhabditis elegans Genotype: chd-3(eh4) X. Description: T14G8.1 Deletion of 2018 bp of T14G8.1 [nucleotide 3699 (in exon 4) joined to 1682 (in exon 6)]. No phenotype observed. Mutagen: EMS Outcrossed: x4 Made by: Received: 06/01/99 Sanger Centre, Hinxton, Cambridge, England ----------------------------------------------------------------------------- Strain: HY123 Species: C. elegans Genotype: nhr-31(ye123) IV. Description: Maintain at 15C. Temperature-sensitive: slow growth rate, reduced brood size. Resistant to Cry proteins. Isolated from EMS screen in N2 background. Reference: Kim YM, et al. PLoS Pathog. 2024 Oct 18;20(10):e1012611. doi: 10.1371/journal.ppat.1012611. PMID: 39423230. Mutagen: EMS Outcrossed: x4 Made by: Youmie Kim Received: 11/14/24 University of Massachusetts Medical School ----------------------------------------------------------------------------- Strain: HY463 Species: Caenorhabditis elegans Genotype: unc-32(e189) pod-1(ye11)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, Sterile Dpys and Uncs. Unc worms produce only dead embyros due to strict maternal effect pod-1(ye11) mutation. Embryos from pod-1 homozygous hermaphrodites are osmotically sensitive and exhibit polarity defects. Mutagen: EMS Outcrossed: x10 Made by: Chad Rappleye Received: 11/01/99 University of Massachusetts Medical School ----------------------------------------------------------------------------- Strain: HY483 Species: Caenorhabditis elegans Genotype: bre-3(ye26) III. Description: Resistant to Cry5B Bt toxin. Mutagen: EMS Outcrossed: x2 Made by: Elyassnia/Aroian Received: 03/01/01 University of Massachusetts Medical School ----------------------------------------------------------------------------- Strain: HY485 Species: Caenorhabditis elegans Genotype: bre-4(ye27) IV. Description: Resistant to Cry5B Bt toxin. See also WBPaper00004776. Mutagen: EMS Outcrossed: x2 Made by: Elyassnia/Aroian Received: 03/01/01 University of Massachusetts Medical School ----------------------------------------------------------------------------- Strain: HY494 Species: Caenorhabditis elegans Genotype: bre-2(ye31) III. Description: Resistant to Cry5B Bt toxin. Reduced brood size. Mutagen: EMS Outcrossed: x2 Made by: Elyassnia/Aroian Received: 03/01/01 University of Massachusetts Medical School ----------------------------------------------------------------------------- Strain: HY496 Species: Caenorhabditis elegans Genotype: bre-1(ye4) IV. Description: Resistant to Cry5B Bt toxin. Reduced brood size. Mutagen: EMS Outcrossed: x2 Made by: Elyassnia/Aroian Received: 03/01/01 University of Massachusetts Medical School ----------------------------------------------------------------------------- Strain: HY498 Species: Caenorhabditis elegans Genotype: bre-5(ye17) I. Description: Resistant to Cry5B Bt toxin. Mutagen: EMS Outcrossed: x2 Made by: Elyassnia/Aroian Received: 03/01/01 University of Massachusetts Medical School ----------------------------------------------------------------------------- Strain: HY520 Species: Caenorhabditis elegans Genotype: pod-2(ye60) II. Description: Cold sensitive embryonic lethal. 95% viable at 24C, 3.7% viable at 15C. 43% symmetric 2-cell. Osmotically senstive (thus requires lineage in osmotically conditioned media, such as 0.8X egg salt). Mutagen: EMS Outcrossed: x4 Made by: Akiko Tagawa Received: 08/13/01 University of Massachusetts Medical School ----------------------------------------------------------------------------- Strain: HY601 Species: Caenorhabditis elegans Genotype: mat-3(or344) III. Description: Temperature-sensitive embryonic lethal, maintain at 15C. Shift L4 hermaphrodites to 25C overnight for mutant embryos. Mutant embryos are sensitive to osmolarity and pressure. Previously called pod-4(or344). Mutagen: EMS Outcrossed: x4 Made by: C. Rappleye Received: 02/20/02 University of Massachusetts Medical School ----------------------------------------------------------------------------- Strain: HY604 Species: Caenorhabditis elegans Genotype: mat-1(ye121) I. Description: Temperature-sensitive embryonic lethal, maintain at 15C. Shift L4 hermaphrodites to 25C overnight for mutant embryos. Mutant embryos are sensitive to osmolarity and pressure. Previously called pod-5(ye121). Mutagen: EMS Outcrossed: x4 Made by: C. Rappleye Received: 02/20/02 University of Massachusetts Medical School ----------------------------------------------------------------------------- Strain: HY607 Species: Caenorhabditis elegans Genotype: apc-1(or319) II. Description: Temperature-sensitive embryonic lethal, maintain at 15C. Shift L4 hermaphrodites to 24C overnight for mutant embryos. Mutant embryos are sensitive to osmolarity and pressure. Previously called pod-3 and mat-2. Mutagen: EMS Outcrossed: x4 Made by: C. Rappleye Received: 02/20/02 University of Massachusetts Medical School ----------------------------------------------------------------------------- Strain: HY618 Species: Caenorhabditis elegans Genotype: apc-1(or374) II; him-5(e1490) V. Description: Temperature-sensitive embryonic lethal, maintain at 15C. Shift L4 hermaphrodites to 25C overnight for mutant embryos. Mutant embryos are sensitive to osmolarity and pressure. Previously called pod-3 and mat-2.. Mutagen: EMS Outcrossed: x4 Made by: C. Rappleye Received: 02/20/02 University of Massachusetts Medical School ----------------------------------------------------------------------------- Strain: HY619 Species: Caenorhabditis elegans Genotype: apc-1(or385) II; him-5(e1490) V. Description: Temperature-sensitive embryonic lethal, maintain at 15C. Shift L4 hermaphrodites to 25C overnight for mutant embryos. Mutant embryos are sensitive to osmolarity and pressure. Previously called pod-3 and mat-2. Mutagen: EMS Outcrossed: x4 Made by: C. Rappleye Received: 02/20/02 University of Massachusetts Medical School ----------------------------------------------------------------------------- Strain: HY621 Species: Caenorhabditis elegans Genotype: emb-27(ye143) II. Description: Temperature-sensitive embryonic lethal, maintain at 15C. Shift L4 hermaphrodites to 25C overnight for mutant embryos. Mutant embryos are sensitive to osmolarity and pressure. Previously called pod-6(ye143). Mutagen: EMS Outcrossed: x4 Made by: C. Rappleye Received: 02/20/02 University of Massachusetts Medical School ----------------------------------------------------------------------------- Strain: HZ107 Species: Caenorhabditis elegans Genotype: him-5(e1490) bxIs13 V; nfya-1(bp4) X. Description: bxIs13 [egl-5::GFP + lin-15(+)]. Him. Unc. Ectopic expression of GFP in head neurons. Mutagen: EMS Outcrossed: x2 Made by: Hong Zhang Received: 06/14/07 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ111 Species: Caenorhabditis elegans Genotype: muIs16 II; sor-1(bp_1)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-5(e1490) V. Description: muIs16 [mab-5::GFP]. Larval lethal at L3/L4 stage. Ectopic expression of GFP in the arrested sor-1 mutant homozygotes (L3 stage). NOTE: This allele is published as bp1, but has been curated in WormBase as bp_1 in order to differentiate it from the STS marker bP1. Mutagen: EMS Outcrossed: x0 Made by: Hong Zhang Received: 06/14/07 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ112 Species: Caenorhabditis elegans Genotype: muIs16 II; sor-1(bp3)/qC1 [dpy-19(e1259) glp-1(q339)] III; him-5(e1490) V. Description: muIs16 [mab-5::GFP]. Larval lethal at L3/L4 stage. Ectopic expression of GFP in the arrested sor-1 mutant homozygotes (L3 stage). Mutagen: EMS Outcrossed: x0 Made by: Hong Zhang Received: 06/14/07 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ113 Species: Caenorhabditis elegans Genotype: him-5(e1490) bxIs13 V; nfya-1(bp5) X. Description: bxIs13 [egl-5::GFP + lin-15(+)]. Him. Unc. Ectopic expression of GFP in head neurons. Mutagen: EMS Outcrossed: x1 Made by: Hong Zhang Received: 06/14/07 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ1569 Species: Caenorhabditis elegans Genotype: bpIs239. Description: bpIs239 [W07G4.5p::W07G4.5::GFP + unc-76(+)]. W07G4.5::GFP is expressed in the intestine. A few GFP aggregates are formed in wild-type embryos at the four-fold stage; the number of aggregates is dramatically increased in epg-7 and atg-3 mutants. Reference: Lin L, et al. J Cell Biol. 2013 Apr 1;201(1):113-29. Mutagen: Gamma radiation Outcrossed: x2 Made by: Hong Zhang Received: 04/27/15 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ1683 Species: Caenorhabditis elegans Genotype: him-5(e1490) V; atg-2(bp576) X. Description: Him; ~30% male. Mutagen: EMS Outcrossed: x2 Made by: Hong Zhang Received: 07/01/13 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ1684 Species: Caenorhabditis elegans Genotype: atg-3(bp412) IV; him-5(e1490) V. Description: Him; ~30% male. Mutagen: EMS Outcrossed: x2 Made by: Hong Zhang Received: 07/01/13 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ1685 Species: Caenorhabditis elegans Genotype: atg-4.1(bp501) I. Description: Mutagen: EMS Outcrossed: x2 Made by: Hong Zhang Received: 07/01/13 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ1686 Species: Caenorhabditis elegans Genotype: bnIs1 I; atg-7(bp411) IV; him-5(e1490) V. Description: bnIs1 [pie-1p::GFP::pgl-1 + unc-119(+)] I. Him; ~30% male. Accumulation of GFP::PGL-1 aggregates in somatic cells in embryos. Mutagen: EMS Outcrossed: x2 Made by: Hong Zhang Received: 07/01/13 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ1687 Species: Caenorhabditis elegans Genotype: atg-9(bp564) him-5(e1490) V. Description: Him; ~30% male. Mutagen: EMS Outcrossed: x2 Made by: Hong Zhang Received: 07/01/13 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ1688 Species: Caenorhabditis elegans Genotype: atg-13(bp414) III. Description: Mutagen: EMS Outcrossed: x2 Made by: Hong Zhang Received: 07/01/13 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ1690 Species: Caenorhabditis elegans Genotype: epg-6(bp242) III; him-5(e1490) V. Description: Him; ~30% male. Mutagen: EMS Outcrossed: x2 Made by: Hong Zhang Received: 07/01/13 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ1691 Species: Caenorhabditis elegans Genotype: epg-8(bp251) I; him-5(e1490) V. Description: Him; ~30% male. Mutagen: EMS Outcrossed: x2 Made by: Hong Zhang Received: 07/01/13 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ1692 Species: Caenorhabditis elegans Genotype: epg-9(bp320) IV. Description: Him; ~30% male. Mutagen: EMS Outcrossed: x2 Made by: Hong Zhang Received: 07/01/13 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ194 Species: Caenorhabditis elegans Genotype: apr-1(bp298) I; wIs51 V. Description: wIs51 [SCMp::GFP + unc-119(+)] V. GFP expression in seam cells. apr-1(bp298) is a G338E missense mutation disrupts the normal asymmetric division pattern of seam cells, causing symmetric divisions. Reference: Huang XX, et al. Dev Biol. 2009 Sep 15;333(2):337-47. Mutagen: EMS Outcrossed: x2 Made by: Hong Zhang Received: 02/16/16 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ455 Species: Caenorhabditis elegans Genotype: him-5(e1490) V; bpIs131. Description: bpIs131 [sepa-1p::sepa-1::GFP + unc-76(+)]. Him. SEPA-1::GFP aggregates form in a temporal pattern. Diffuse SEPA-1::GFP is detectible in most cells at the comma stage and greatly diminished by the 2-fold stage. After hatching, cytoplasmic SEPA-1::GFP aggregates were found in a few unidentified cells in the head and tail regions and also in the intestine, especially in the anterior and posterior pairs of intestine cells. Reference: Tian Y, et al. Cell. 2010 Jun 11;141(6):1042-55. Mutagen: Gamma radiation Outcrossed: x2 Made by: Hong Zhang Received: 04/27/15 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ470 Species: Caenorhabditis elegans Genotype: sea-2(bp283) II; wIs51 V. Description: wIs51 [SCMp::GFP + unc-119(+)] V. GFP expression in seam cells. Mutagen: EMS Outcrossed: x2 Made by: Hong Zhang Received: 07/01/13 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ504 Species: Caenorhabditis elegans Genotype: him-5(e1490) V; bpIs37. Description: bpIs37 [dcap-1p::dcap-1::DsRed + rol-6(su1006)]. Rollers. Translational DsRed reporter for the P body-specific marker dcap-1, which encodes the C. elegans ortholog of decapping complex component DCAP1. Low levels of expression are homogenously distributed in the cytoplasm at all stages of embryogenesis. Reference: Sun YY, et al. Protein Cell. 2011 Nov;2(11):918-39. Mutagen: Gamma radiation Outcrossed: x2 Made by: Hong Zhang Received: 01/07/16 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ589 Species: Caenorhabditis elegans Genotype: him-5(e1490) V; bpIs151. Description: bpIs151 [sqst-1p::sqst-1::GFP + unc-76(+)]. Him. In wild-type embryos, sqst-1::GFP is very weakly expressed and diffusely localized in the cytoplasm. Reference: Tian Y, et al. Cell. 2010 Jun 11;141(6):1042-55. Mutagen: Gamma radiation Outcrossed: x2 Made by: Hong Zhang Received: 04/27/15 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ620 Species: Caenorhabditis elegans Genotype: ceh-16(bp323) III; wIs51 V. Description: wIs51 [SCMp::GFP + unc-119(+)] V. GFP expression in seam cells. Temperature-sensitive: maintain at 20C or lower. bp323 mutants show reduced number of seam cells when raised at restrictive temperature (25C). Reference: Huang XX, et al. Dev Biol. 2009 Sep 15;333(2):337-47. Mutagen: EMS Outcrossed: x2 Made by: Hong Zhang Received: 02/16/16 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ66 Species: Caenorhabditis elegans Genotype: sop-2(bp186) II; him-5(e1490) bxIs16 V. Description: bxIs16 [cat-2::YFP + tph-1::CFP]. Temperature sensitive: lethal at 25C. Maintain at 20C. Mutagen: EMS Outcrossed: x1 Made by: Hong Zhang Received: 06/14/07 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ67 Species: Caenorhabditis elegans Genotype: him-5(e1490) bxIs16 V; sor-3(bp185) X. Description: bxIs16 [cat-2::YFP + tph-1::CFP]. Temperature sensitive: lethal at 25C. Maintain at 20C. Mutagen: EMS Outcrossed: x2 Made by: Hong Zhang Received: 06/14/07 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ769 Species: Caenorhabditis elegans Genotype: him-5(e1490) V; bpIs88. Description: bpIs88 [tia-1p::tia-1::GFP + dcap-1p::dcap-1::RFP + rol-6(su1006)]. Rollers. Rolling phenotype is more apparent when raised >20C. Reference: Sun YY, et al. Protein Cell. 2011 Nov;2(11):918-39. Mutagen: Gamma radiation Outcrossed: x2 Made by: Hong Zhang Received: 01/07/16 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ771 Species: Caenorhabditis elegans Genotype: him-5(e1490) V; bpIs90. Description: bpIs90 [tia-1p::tia-1::GFP + rol-6(su1006)]. Rollers. Rolling phenotype is more apparent when raised >20C. TIA-1::GFP is homogenously distributed in the cytoplasm during embryogenesis. At larval stages, diffuse TIA-1::GFP signals are observed in seam cells and hypodermal cells. TIA-1::GFP forms distinct bodies, especially in seam cells and hypodermal cells, under stress conditions. Reference: Sun YY, et al. Protein Cell. 2011 Nov;2(11):918-39. Mutagen: Gamma radiation Outcrossed: x2 Made by: Hong Zhang Received: 01/07/16 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: HZ946 Species: Caenorhabditis elegans Genotype: rpl-43(bp399) II; bpIs151. Description: bpIs151 [sqst-1p::sqst-1::GFP + unc-76(+)]. bp399 mutants accumulate SQST-1 aggregates strictly in the intestine in a distinct temporal pattern. SQST-1::GFP aggregates are absent in bp399 embryos, but start to form in L1 larvae and increase in number and size throughout larval development. Reference: Guo B, et al. EMBO Rep. 2014 Jun;15(6):705-13. Mutagen: EMS Outcrossed: x2 Made by: Hong Zhang Received: 04/27/15 Institute of Biophysics, Chinese Academy of Sciences, Beijing, China ----------------------------------------------------------------------------- Strain: IA123 Species: Caenorhabditis elegans Genotype: cdc-25.1(ij48) I; unc-76(e911) ijIs10 V. Description: ijIs10 [cpr-5::GFP::lacZ + unc-76(+)]. Extra intestinal cells. Mutagen: EMS Outcrossed: x3 Made by: Caroline Clucas Received: 04/06/04 University of Glasgow, Scotland ----------------------------------------------------------------------------- Strain: IA260 Species: Caenorhabditis elegans Genotype: unc-51(e369) rol-9(sc148)/sec-23(ij13) V. Description: Heterozygotes are WT and segregate WT, Roller Unc and embryonic lethals. Mutagen: EMS Outcrossed: x3 Made by: Brett Roberts Received: 04/06/04 University of Glasgow, Scotland ----------------------------------------------------------------------------- Strain: IB16 Species: Caenorhabditis elegans Genotype: ceh-17(np1) I. Description: WT behavioral phenotype. Axon guidance defect in ceh-17 expressing neurons ALA and 4SIA. ceh-17 = D1007.1, a C. elegans paired homeodomain transcription factor, Phox2 orthologue. np1 is a molecular null. np1 is a 1353 bp deletion inculding 549 bp upstream of the initiator ATG and extending to the 5th codon of the homeodomain. [NOTE: Miyazaki, et al. (2022) report that this strain carries the fln-2(ot611) mutation in the background, and outcrossing the strain resulted in significantly reduced quiescence during lethargus.] Mutagen: UV/TMP Outcrossed: x3 Made by: Nathalie Pujol Received: 12/01/00 IBDM, Marseille, France ----------------------------------------------------------------------------- Strain: IC136 Species: Caenorhabditis elegans Genotype: zdIs5 I; vab-1(dx31) II. Description: zdIs5 [mec-4p::GFP + lin-15(+)] I. Animals contain GFP expressed in the touch neurons and have a notched head phenotype. Mutagen: Outcrossed: x Made by: Ahmed Mohamed Received: 05/17/06 Queen's University, Kingston, ON, Canada ----------------------------------------------------------------------------- Strain: IC156 Species: Caenorhabditis elegans Genotype: zdIs5 I; quIs4. Description: zdIs5 [mec-4p::GFP + lin-15(+)] I. quIs4 [mec-4::myr::vab-1(G912E) + rol-6(su1006)]. Animals contain GFP expressed in the touch neurons. Mutagen: UV/TMP Outcrossed: x2 Made by: Ahmed Mohamed Received: 05/17/06 Queen's University, Kingston, ON, Canada ----------------------------------------------------------------------------- Strain: IC361 Species: Caenorhabditis elegans Genotype: vab-1(e2)/mIn1 [dpy-10(e128) mIs14] II; sax-3(ky123) X. Description: Heterozygotes are WT and GFP+. mIn1 homozygotes are Dpy and GFP+. vab-1; sax-3 homozygotes are synthetic lethal. Mutagen: Outcrossed: x Made by: Simona Ghenea Received: 01/19/06 Queen's University, Kingston, ON, Canada ----------------------------------------------------------------------------- Strain: IC400 Species: Caenorhabditis elegans Genotype: zdIs5 I; quIs5 II; him-5(e1490) V. Description: zdIs5 [mec-4p::GFP + lin-15(+)] I. quIs5 [mec-4::myr::vab-1 + rol-6(su1006)]. Animals contain GFP expressed in the touch neurons. Mutagen: UV/TMP Outcrossed: x2 Made by: Ahmed Mohamed Received: 05/17/06 Queen's University, Kingston, ON, Canada ----------------------------------------------------------------------------- Strain: IC459 Species: Caenorhabditis elegans Genotype: sax-3(ky123) X; quEx102. Description: quEx102[F25B3.3::SAX-3 + odr-1::RFP]. F25B3.3::SAX-3 partially rescues the lethality and notch phenotype of sax-3(ky123). Maintain by picking RFP+. Mutagen: Outcrossed: x Made by: Simona Ghenea Received: 01/19/06 Queen's University, Kingston, ON, Canada ----------------------------------------------------------------------------- Strain: IC464 Species: Caenorhabditis elegans Genotype: sax-3(ky123) X; quEx100. Description: quEx100 [ajm-1::sax-3 + odr-1::RFP]. ajm-1::sax-3 partially rescues the lethality of sax-3(ky123). Maintain by picking RFP+. Mutagen: Outcrossed: x Made by: Simona Ghenea Received: 01/19/06 Queen's University, Kingston, ON, Canada ----------------------------------------------------------------------------- Strain: IC476 Species: Caenorhabditis elegans Genotype: sax-3(ky123) X; quEx99. Description: quEx99 [sax-3(minigene) + odr-1::RFP]. Rescues the lethality of ky123. Pick RFP+ to maintain. Mutagen: Outcrossed: x Made by: Received: 06/23/09 Queen's University, Kingston, ON, Canada ----------------------------------------------------------------------------- Strain: IC611 Species: Caenorhabditis elegans Genotype: quEx128. Description: quEx128 [npr-9::GFP + rol-6(su1006)]. Maintain by picking Rollers. ZK455.3 is npr-9 and is expressed in AIB neuron. Mutagen: Outcrossed: x Made by: Ian Chin-Sang Received: 02/18/08 Queen's University, Kingston, ON, Canada ----------------------------------------------------------------------------- Strain: IC683 Species: Caenorhabditis elegans Genotype: npr-9(tm1652) X. Description: Mutagen: Outcrossed: x2 Made by: Ian Chin-Sang Received: 02/18/08 Queen's University, Kingston, ON, Canada ----------------------------------------------------------------------------- Strain: IC692 Species: Caenorhabditis elegans Genotype: quEx162. Description: quEx162 [sax-3p::GFP + rol-6(su1006)]. Pick GFP to maintain. Very bright GFP in early embryos (after 24 cell stage). Not all GFP+ worms are rolling. Mutagen: Outcrossed: x Made by: Tony Papanicolaou Received: 12/02/08 Queen's University, Kingston, ON, Canada ----------------------------------------------------------------------------- Strain: IC699 Species: Caenorhabditis elegans Genotype: sax-3(ky123) ; quEx168. Description: quEx168 [sax-3::GFP + odr-1::RFP]. Pick RFP+ to maintain. GFP is visible at higher magnification but fades quickly. Strongest GFP is in the head region. The Chin-Sang Lab recommends researchers use this strain instead of IC450, as sax-3::GFP expression in IC699 is more consistent than in the comparable strain IC450. Mutagen: Outcrossed: x Made by: Tony Papanicolaou Received: 12/02/08 Queen's University, Kingston, ON, Canada ----------------------------------------------------------------------------- Strain: IC700 Species: Caenorhabditis elegans Genotype: juIs73 II; vab-2(ju1) IV. Description: juIs73 [unc-25p::GFP] II. Mutagen: Outcrossed: x Made by: Ian Chin-Sang Received: 04/05/07 Queen's University, Kingston, ON, Canada ----------------------------------------------------------------------------- Strain: IC765 Species: Caenorhabditis elegans Genotype: npr-9(tm1652) X; quEx182. Description: quEx182 [npr-9(+) + sur-5::GFP + odr-1::RFP]. Maintain by picking GFP+. Rescuing npr genomic fragment co-injected with sur-5::GFP and odr-1::RFP]. transgenic (GFP+) animals tend to wander off the food. Mutagen: Outcrossed: x Made by: Chin-Sang Lab Received: 02/18/08 Queen's University, Kingston, ON, Canada ----------------------------------------------------------------------------- Strain: IE53215 Species: Caenorhabditis elegans Genotype: ttTi53215 V. Description: Homozygous. Mutagen: Outcrossed: x Made by: Mos1 mutagenesis Received: 02/04/09 Brown University ----------------------------------------------------------------------------- Strain: IG10 Species: Caenorhabditis elegans Genotype: tol-1(nr2033) I. Description: Healthy and fertile but exhibit a lowly penetrant lethality, and a small but significant proportion of the mutants arrest as early larvae. Reference: Pujol N, et al. Curr Biol. 2001 Jun 5;11(11):809-21. Mutagen: Outcrossed: x2 Made by: Received: 02/17/04 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG1107 Species: Caenorhabditis elegans Genotype: sta-2(fr67) V; frIs7 IV. Description: frIs7 [nlp-29p::GFP + col-12p::DsRed] IV. Blocks inducible expression of antimicrobial peptide nlp-29 after D. coniospara infection or treatment with PMA. Maintain under normal conditions. Reference: Labed Sa, et al. PLoS One. 2012 7(3):e33887. Mutagen: EMS Outcrossed: x0 Made by: Nathalie Pujol Received: 03/30/12 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG1110 Species: Caenorhabditis elegans Genotype: snf-12(fr70) X; frIs7 IV. Description: frIs7 [nlp-29p::GFP + col-12p::DsRed] IV. Blocks inducible expression of antimicrobial peptide nlp-29 after D. coniospara infection or treatment with PMA. Maintain under normal conditions. Reference: Labed Sa, et al. PLoS One. 2012 7(3):e33887. Mutagen: EMS Outcrossed: x0 Made by: Nathalie Pujol Received: 03/30/12 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG1114 Species: Caenorhabditis elegans Genotype: snf-12(fr74) X; frIs7 IV. Description: frIs7 [nlp-29p::GFP + col-12p::DsRed] IV. Blocks inducible expression of antimicrobial peptide nlp-29 after D. coniospara infection or treatment with PMA. Maintain under normal conditions. Reference: Labed Sa, et al. PLoS One. 2012 7(3):e33887. Mutagen: EMS Outcrossed: x0 Made by: Nathalie Pujol Received: 03/30/12 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG113 Species: Caenorhabditis elegans Genotype: frP1 III. Description: Mos1 transposon insertion: F28F5 (at position 1023) acacctggtaCTCACCCCTGTTTAACACACAGTCTTCACA. Mos1 sequence is in lowercase. Mutagen: Mos1 Outcrossed: x0 Made by: Anne Millet Received: 10/31/02 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG114 Species: Caenorhabditis elegans Genotype: frP2 III. Description: Mos1 transposon insertion: T28A8 (at position 2841) acacctggtaTTAAGAAAAAAACCGAGTCCTCTCACCGTA. Mos1 sequence is in lowercase. Mutagen: Mos1 Outcrossed: x0 Made by: Anne Millet Received: 10/31/02 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG115 Species: Caenorhabditis elegans Genotype: frP3 V. Description: Mos1 transposon insertion: CO8D8 (at position 17534) acacctggtaATAAAAGAAGGAAAAATAAATATTTAAGTT. Mos1 sequence is in lowercase. Mutagen: Mos1 Outcrossed: x0 Made by: Anne Millet Received: 10/31/02 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG117 Species: Caenorhabditis elegans Genotype: frP41 IV. Description: Mos1 transposon insertion: Y38C1AB (at position 8906) acacctggtaCAACTGGTTTAGAATAATTTAGAAATTACAA. Mos1 sequence is in lowercase. Mutagen: Mos1 Outcrossed: x0 Made by: Anne Millet Received: 10/31/02 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG118 Species: Caenorhabditis elegans Genotype: frP5 V. Description: Mos1 transposon insertion: F57F4 (at position 15459) acacctggtaGTCTCTTCAAGGAAGAACAATGAGAAATAA. Mos1 sequence is in lowercase. Mutagen: Mos1 Outcrossed: x0 Made by: Anne Millet Received: 10/31/02 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG119 Species: Caenorhabditis elegans Genotype: frP7 IV; frP6 X. Description: Mos1 transposon insertion: frP6: F39H12 (at position 19268) acacctggtaAGCATAGGGCTAGGCCTTAGACTTTATATT, and frP7: Y10G11A (at position 1294) acacctggtaAAACAATTTCCAAGTAAAAAAATCATGTATT. Mos1 sequence is in lowercase. Mutagen: Mos1 Outcrossed: x0 Made by: Anne Millet Received: 10/31/02 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG122 Species: Caenorhabditis elegans Genotype: frP8 frP9 III. Description: Mos1 transposon insertion: frP8: H14A12 (at position 9047) acacctggtaTACAATTTTGATTTCAGAAAGTTCTCTGAC, and frP9: Y45F3A (at position 220) acacctggtaGAATTGTTCGAACAAGCTTCAACGAGAAAGCAA. Mos1 sequence is in lowercase. Mutagen: Mos1 Outcrossed: x0 Made by: Anne Millet Received: 10/31/02 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG123 Species: Caenorhabditis elegans Genotype: frP10 X. Description: Mos1 transposon insertion: B0272 (at position 11068) acacctggtaTATGAGAAAATCTATAGCAAGTACATATCT. Mos1 sequence is in lowercase. Mutagen: Mos1 Outcrossed: x0 Made by: Anne Millet Received: 10/31/02 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG124 Species: Caenorhabditis elegans Genotype: frP11 I; frP13 II; frP14 III; frP12 X. Description: Mos1 transposon insertions: frP11: F31C3 (at position 26364) acacctggtaTCGGAGGAGCTGCCAAATGGCGTCCGACTT. frP12: F01E11 (at position 3748) acacctggtaCAATCGAAAATCATTGAAATCAGTTAACAG. frP13: Y38E10A (at position 40384) acacctggtaTAGCTAGAGGACTGTCCCGGTCTAAAATGA. frP14: F53A2 (at position 34332) acacctggtaTATTATAAAAGATGTATGAAATGTCGTGAA. Mos1 sequence is in lowercase. Mutagen: Mos1 Outcrossed: x0 Made by: Anne Millet Received: 10/31/02 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG1241 Species: Caenorhabditis elegans Genotype: sta-2(ok1860) V. Description: Strain derived by outcrossing RB1547 two times to N2 to remove the lethal background mutation in RB1547. Reference: Dierking K, et al. Cell Host Microbe. 2011 May 19;9(5):425-35. Mutagen: Outcrossed: x2 Made by: Nathalie Pujol Received: 12/02/13 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG125 Species: Caenorhabditis elegans Genotype: frP15 IV. Description: Mos1 transposon insertion: Y97E10B (at position 1022) acacctggtaAAACATGCTGAAAGTTTACTAAAATTGAAT. Mos1 sequence is in lowercase. Mutagen: Mos1 Outcrossed: x0 Made by: Anne Millet Received: 10/31/02 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG126 Species: Caenorhabditis elegans Genotype: frP16 III. Description: Mos1 transposon insertion: Y39A3CL (at position 24032) acacctggtaTATCGAAAAAAAATTTTTTTTTGGAATTTT. Mos1 sequence is in lowercase. Mutagen: Mos1 Outcrossed: x0 Made by: Anne Millet Received: 10/31/02 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG127 Species: Caenorhabditis elegans Genotype: frP17 IV. Description: Mos1 transposon insertion: K07H8 (at position 8196) acacctggtaTTATCAAAGTTAGAATTCAAACTGCGTTGC. Mos1 sequence is in lowercase. Mutagen: Mos1 Outcrossed: x0 Made by: Anne Millet Received: 10/31/02 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG128 Species: Caenorhabditis elegans Genotype: frP20 frP19 II; frP18 V. Description: Mos1 transposon insertions: frP18: K03H4 (at position 19156) acacctggtaGAATGGATGAGGTTGAAAGTGACGAAGAAAA. frP19: F35H8 (at position 15891) acacctggtaGTTTACTCATATTTCTTTCCTCTCTTCTTC. frP20: T14B4 (at position 25648) acacctggtaTGCAAATATGGTTAATGTAAGGCATTTTTG. Mos1 sequence is in lowercase. Mutagen: Mos1 Outcrossed: x0 Made by: Anne Millet Received: 10/31/02 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG129 Species: Caenorhabditis elegans Genotype: frP21 IV. Description: Dumpy. Mos1 transposon insertion: F30B5 (at position 22345) (dpy-13) acacctggtaGTTGTAGACGATTGGAAGAGTAATGCAAAC. Mos1 sequence is in lowercase. Mutagen: Mos1 Outcrossed: x0 Made by: Anne Millet Received: 10/31/02 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG130 Species: Caenorhabditis elegans Genotype: tol-1(nr2013) I. Description: Maintain at 15C. At 25C, nr2013 homozygotes are not viable. At 15C, less than 10% of nr2013 homozygotes develop into adults that are marginally fertile, while the majority of worms arrest at different developmental stages and exhibit dramatic defects in morphogenesis. Reference: Pujol N, et al. Curr Biol. 2001 Jun 5;11(11):809-21. Mutagen: Outcrossed: x2 Made by: Received: 03/01/04 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG1335 Species: C. elegans Genotype: frEx479. Description: frEx479 [F57F4.4p::GFP + col-12p::DsRed]. Pick DsRed+ animals to maintain array. Constitutive GFP expression in posterior intestinal cells induced upon infection with Photorhabdus luminescens in posterior intestinal cells. Reference: Julien-Gau I, et al. Dev Comp Immunol. 2014 Feb;42(2):132-7. Mutagen: Outcrossed: x0 Made by: L. Kurz Received: 10/30/18 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG1352 Species: Caenorhabditis elegans Genotype: nipi-4(fr71) V; frIs7 IV. Description: frIs7 [nlp-29p::GFP + col-12p::DsRed] IV. Blocks inducible expression of antimicrobial peptide nlp-29 after D. coniospara infection or treatment with PMA. Maintain under normal conditions. Reference: Labed Sa, et al. PLoS One. 2012 7(3):e33887. Mutagen: EMS Outcrossed: x2 Made by: Nathalie Pujol Received: 03/30/12 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG1839 Species: C. elegans Genotype: frSi17 II; frIs7 IV; rde-1(ne300) V. Description: frSi17 [mtl- 2p::rde-1 3'UTR] II. frIs7 [nlp-29p::GFP + col-12p::DsRed] IV. frSi17 inserted into ttTi5605 site using CRISPR/Cas9 engineering. RDE-1 activity is rescued in the intestine, making animals RNAi-deficient except for intestinal tissues. The frSi17 insertion can be detected using a primer within the mtl-2 promoter (jep1061: aacaaacgtgggatgtaacc) in combination with downstream primer in rde-1 (jep2817 tcatactcgtagtattcccg), producing a 786 bp product if insertion is present. rde-1(ne300) can be genotyped by sequencing the PCR product from jep2299: gaacaacgacaatcgagcacca and jep3108: ATcttgtgaccgaactgtcc. (jep3108 is not present in the frSi17 transgene) Reference: Watts JS, et al. G3 (Bethesda) 2020 Nov 5;10(11):4167-4176. PMID: 32943454 Mutagen: Outcrossed: x0 Made by: Nathalie Pujol Received: 10/20/20 Washington State University, Pullman WA ----------------------------------------------------------------------------- Strain: IG1846 Species: C. elegans Genotype: frSi21 II; frIs7 IV; rde-1(ne300) V. Description: frSi21 [col-62p::rde-1 3'UTR] II. frIs7 [nlp-29p::GFP + col-12p::DsRed] IV. frSi21 inserted into ttTi5605 site using CRISPR/Cas9 engineering. RDE-1 activity is rescued in adult epidermal tissues, making animals RNAi-deficient except for hypodermal (skin) tissues from the young adult stage. The frSi21 insertion can be detected using a primer within the col-62 promoter (jep2245: caaaaaggcgggatgagcag) in combination with downstream primer in rde-1 (jep2817 tcatactcgtagtattcccg), producing a 965 bp product if insertion is present. rde-1(ne300) can be genotyped by sequencing the PCR product from jep2299: gaacaacgacaatcgagcacca and jep3108: ATcttgtgaccgaactgtcc (jep3108 is not present in the frSi21 transgene). Reference: Watts JS, et al. G3 (Bethesda) 2020 Nov 5;10(11):4167-4176. PMID: 32943454 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Nathalie Pujol Received: 10/20/20 Washington State University, Pullman WA ----------------------------------------------------------------------------- Strain: IG2212 Species: C. elegans Genotype: spia-1(fr201[spia-1::mNG(internal)::3xFLAG] X. Description: Internal mNeonGreen::3xFLAG tags inserted into endogenous spia-1 locus. Mutagen: Outcrossed: x0 Made by: Jordan Ward, Nathalie Pujol Received: 10/04/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: IG256 Species: Caenorhabditis elegans Genotype: xnp-1(tm678) I. Description: Temperature sensitive. Sterile at 25C. Larval lethal with lin-35 and hlp-2. The deletion extends 673 bp (and not 674 + 1 insertion as described on the S. Mitani website). The deletion breakpoint is AAAAAAAGAGCTGAAACATCGGAAGAGTCA/AGATGCAGAGAGAGCAGAGAAAGAGAGA AGA. B0041.7 Reference: Cardoso C, et al. Dev Biol. 2005 Feb 1;278(1):49-59. Mutagen: UV/TMP Outcrossed: x3 Made by: N. Pujol Received: 04/29/05 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG274 Species: Caenorhabditis elegans Genotype: frIs7. Description: frIs7 [nlp-29p::GFP + col-12p::DsRed] IV. The nlp-29p::GFP reporter is induced in the epidermis upon infection with Drechmeria coniospora, wounding and osmotic stress. Reference: Pujol N, et al. Curr Biol. 2008 Apr 8;18(7):481-9. Mutagen: Gamma Rays Outcrossed: x3 Made by: Nathalie Pujol Received: 09/08/08 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG339 Species: Caenorhabditis elegans Genotype: tpa-1(fr1) frIs7 IV. Description: frIs7 [nlp-29p::GFP + col-12p::DsRed] IV. Displays tpa-1 phenotypes (e.g. resistance to PMA). Isolated in a genetic screen for mutants failing to show an induction of nlp-29p::GFP reporter gene expression upon infection with the fungus Drechmeria coniospora (the Nipi phenotype). References: Pujol N, et al. Curr Biol. 2008 Apr 8;18(7):481-9. Ziegler K, et al. Cell Host Microbe. 2009 Apr 23;5(4):341-52. Mutagen: EMS Outcrossed: x3 Made by: N. Pujol Received: 11/10/15 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG341 Species: Caenorhabditis elegans Genotype: tpa-1(fr3) frIs7 IV. Description: frIs7 [nlp-29p::GFP + col-12p::DsRed] IV. Displays tpa-1 phenotypes (e.g. resistance to PMA). Isolated in a genetic screen for mutants failing to show an induction of nlp-29p::GFP reporter gene expression upon infection with the fungus Drechmeria coniospora (the Nipi phenotype). References: Pujol N, et al. Curr Biol. 2008 Apr 8;18(7):481-9. Ziegler K, et al. Cell Host Microbe. 2009 Apr 23;5(4):341-52. Mutagen: EMS Outcrossed: x3 Made by: N. Pujol Received: 11/10/15 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG342 Species: Caenorhabditis elegans Genotype: frIs7 IV; nipi-3(fr4) X. Description: frIs7 [nlp-29p::GFP + col-12p::DsRed] IV. Slo, Sma, and Dpy at 25C. Reference: Pujol N, et al. Curr Biol. 2008 Apr 8;18(7):481-9. Mutagen: EMS Outcrossed: x2 Made by: Nathalie Pujol Received: 05/27/08 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG348 Species: Caenorhabditis elegans Genotype: fasn-1(fr8) I; frIs7 IV. Description: frIs7 [nlp-29p::GFP + col-12p::DsRed] IV. Constitutive expression of antimicrobial peptide nlp-29. Maintain under normal conditions. Reference: Lee KZ, et al. Virulence. 2010 May-Jun;1(3):113-22. Mutagen: EMS Outcrossed: x3 Made by: Nathalie Pujol Received: 03/30/12 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG358 Species: Caenorhabditis elegans Genotype: oxEx229. Description: oxEx229 [Mos1 Substrate + myo-2::GFP]. Pick GFP+ to maintain. Should be grown at 25C. Reference: Vallin E, et al. PLoS One. 2012;7(2):e30482. Mutagen: Outcrossed: x3 Made by: Yohann Duverger Received: 03/15/25 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG444 Species: Caenorhabditis elegans Genotype: frEx113. Description: frEx113 [(pJL44) transposase + col-12p::DsRed]. Should be grown at 25C. Reference: Vallin E, et al. PLoS One. 2012;7(2):e30482. Mutagen: Outcrossed: x2 Made by: Olivier Zugasti Received: 06/01/12 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG544 Species: Caenorhabditis elegans Genotype: nipi-3(fr4) X. Description: Slo, Sma, and Dpy at 25C. Reference: Pujol N, et al. Curr Biol. 2008 Apr 8;18(7):481-9. Mutagen: EMS Outcrossed: x2 Made by: Nathalie Pujol Received: 05/27/08 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG6 Species: Caenorhabditis elegans Genotype: smf-1(eh5) X. Description: No phenotype. Contains a 2063 bp deletion in the smf-1 locus (K11G12.4) from position 17016 to position 19079 on the K11G12 cosmid. eh5 was obtained from the Sanger Centre Knockout Service in strain HX104. Reference: Au C, et al. PLoS One. 2009 Nov 18;4(11):e7792. Mutagen: Outcrossed: x5 Made by: Received: 02/01/00 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG685 Species: Caenorhabditis elegans Genotype: tir-1(tm3036) III. Description: Reference: Pujol N, et al. PLoS Pathog. 2008 Jul 18;4(7):e1000105. Mutagen: UV/TMP Outcrossed: x2 Made by: Shohei Mitani Received: 05/27/08 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IG692 Species: Caenorhabditis elegans Genotype: tir-1(tm3036) III; frIs7. Description: frIs7 [nlp-29p::GFP + col-12p::DsRed] IV. Reference: Pujol N, et al. PLoS Pathog. 2008 Jul 18;4(7):e1000105. Mutagen: UV/TMP Outcrossed: x2 Made by: Shohei Mitani Received: 05/27/08 Centre d'Immunologie, Marseille, France ----------------------------------------------------------------------------- Strain: IJ1651 Species: Caenorhabditis elegans Genotype: mdt-15(yh44[mdt-15::degron::EmGFP]) III. Description: Degron and EmGFP tag inserted at the 3' end of the endogenous mdt-15 gene locus by CRISPR/Cas9 engineering. This strain can be used for auxin-inducible degradation (AID) of MDT-15 protein. Reference: Lee D, et al. PLoS Biol. 2019 Aug 13;17(8):e3000415. doi: 10.1371/journal.pbio.3000415. PMID: 31408455. Mutagen: Outcrossed: x4 Made by: Dongyeop Lee Received: 06/20/22 Korea Advanced Institute of Science and Technology (KAIST), Yuseong-gu, South Korea ----------------------------------------------------------------------------- Strain: IK105 Species: Caenorhabditis elegans Genotype: pkc-1(nj1) V. Description: Thermophilic. Defective chemotaxis to AWA- and AWC- sensed odorants except for partially defective chemotaxis to pyrazine. Defective chemotaxis to NaCl. Defective osmotic avoidance. PKA ttx-4. Mutagen: EMS Outcrossed: x6 Made by: Okochi & Okumura Received: 07/20/05 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK1191 Species: Caenorhabditis elegans Genotype: eat-16(nj8) I. Description: Reference: Nishio N, et al. Genes Cells. 2012 May;17(5):365-86. Mutagen: EMS Outcrossed: x7 Made by: Okumura & Mori Received: 06/10/13 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK130 Species: Caenorhabditis elegans Genotype: pkc-1(nj3) V. Description: Thermophilic. Defective chemotaxis to AWA- and AWC- sensed odorants except for partially defective chemotaxis to pyrazine. Partially defective chemotaxis to NaCl. Partially defective osmotic avoidance. PKA ttx-4. Mutagen: EMS Outcrossed: x6 Made by: Okochi & Okumura Received: 07/20/05 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK174 Species: Caenorhabditis elegans Genotype: pkc-1(nj4) V. Description: Thermophilic. Defective chemotaxis to AWA- and AWC- sensed odorants except for partially defective chemotaxis to pyrazine. Partially defective chemotaxis to NaCl. Partially defective osmotic avoidance. PKA ttx-4. Mutagen: EMS Outcrossed: x3 Made by: Okochi & Okumura Received: 07/20/05 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK183 Species: Caenorhabditis elegans Genotype: sax-7(nj13) IV. Description: The maintenance of neuronal cell body positions is abnormal. Mutagen: mut-7 Outcrossed: x9 Made by: H Sasakura & I Mori Received: 09/15/05 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK427 Species: Caenorhabditis elegans Genotype: gcy-23(nj37) IV. Description: Nearly normal thermotaxis behavior. Mutagen: UV/TMP Outcrossed: x10 Made by: Inada/Mori Received: 09/10/08 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK429 Species: Caenorhabditis elegans Genotype: gcy-18(nj38) IV. Description: Nearly normal thermotaxis behavior. Mutagen: UV/TMP Outcrossed: x10 Made by: Inada/Mori Received: 09/10/08 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK537 Species: Caenorhabditis elegans Genotype: sax-7(nj48) IV. Description: The maintenance of neuronal cell body positions is abnormal. Mutagen: TMP/UV Outcrossed: x10 Made by: H Sasakura & I Mori Received: 09/15/05 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK575 Species: Caenorhabditis elegans Genotype: ttx-7(nj40) I. Description: Weak allele. Severe thermotaxis defect. Weak defects in chemitaxis. Subcellular localization of SNB-1:GFP is abnormal in RIA interneurons. Mutagen: EMS Outcrossed: x10 Made by: Received: 01/16/07 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK581 Species: Caenorhabditis elegans Genotype: ins-1(nj32) IV. Description: Reference: Kodama E, et al. Genes Dev. 2006 Nov 1;20(21):2955-60. Mutagen: EMS Outcrossed: x10 Made by: Kodama & Mori Received: 06/10/13 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK589 Species: Caenorhabditis elegans Genotype: ttx-7(nj50) I. Description: Putative null allele which lacks whole of the first exon of the gene (lacking 361 bp area corresponding to 13460-13820 of the cosmid F13G3). Severe thermotaxis defect. Weak defects in chemitaxis. Subcellular localization of synaptic proteins is abnormal in RIA interneurons. Mutagen: TMP/UV Outcrossed: x10 Made by: Received: 01/16/07 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK591 Species: Caenorhabditis elegans Genotype: ttx-7(nj51) I. Description: Putative null allele which lacks whole of the 3rd exon of the gene (lacking 260 bp area corresponding to 12984-13243 of the cosmid F13G3). Severe thermotaxis defect. Weak defects in chemitaxis. Subcellular localization of SNB-1::GFP is abnormal in RIA interneurons. Mutagen: TMP/UV Outcrossed: x10 Made by: Received: 01/16/07 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK597 Species: Caenorhabditis elegans Genotype: gcy-23(nj37) gcy-8(oy44) gcy-18(nj38) IV. Description: Severe defects in thermotaxis. Mutagen: UV/TMP(nj37 & nj38); EMS(oy44) Outcrossed: x Made by: Inada/Mori Received: 09/10/08 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK599 Species: Caenorhabditis elegans Genotype: sax-7(nj52) IV. Description: The maintenance of neuronal cell body positions is abnormal. Mutagen: mut-7 Outcrossed: x6 Made by: H Sasakura & I Mori Received: 09/15/05 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK600 Species: Caenorhabditis elegans Genotype: eat-4(nj2) III. Description: Reference: Ohnishi N, et al. EMBO J. 2011 Apr 6;30(7):1376-88. Mutagen: EMS Outcrossed: x11 Made by: Ohnishi & Mori Received: 06/10/13 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK602 Species: Caenorhabditis elegans Genotype: eat-4(nj6) III. Description: Reference: Ohnishi N, et al. EMBO J. 2011 Apr 6;30(7):1376-88. Mutagen: EMS Outcrossed: x10 Made by: Ohnishi & Mori Received: 10/23/13 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK637 Species: Caenorhabditis elegans Genotype: sax-7(nj53) IV. Description: nj53 is a SAX-7 long-form-specific deletion mutant. nj53 shos the normal neuronal position phenotype. Mutagen: TMP/UV Outcrossed: x6 Made by: H Sasakura & I Mori Received: 09/15/05 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK705 Species: Caenorhabditis elegans Genotype: njIs10. Description: njIs10 [glr-3::GFP]. RIA interneurons express soluble GFP. Mutagen: UV/TMP to integrate Outcrossed: x3 Made by: Y Tanizawa & I Mori Received: 01/16/07 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK716 Species: Caenorhabditis elegans Genotype: njIs11. Description: njIs11[glr-3p::GFP + ges-1p::RFP]. RIA interneurons express soluble GFP. Mutagen: UV/TMP to integrate Outcrossed: x3 Made by: Y Tanizawa & I Mori Received: 01/16/07 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK718 Species: Caenorhabditis elegans Genotype: njIs12. Description: njIs12[glr-3p::glr-1::GFP + glr-3p::RFP + ges-1p::RFP]. RIA interneurons express soluble GFP. Mutagen: UV/TMP to integrate Outcrossed: x3 Made by: Y Tanizawa & I Mori Received: 01/16/07 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK721 Species: Caenorhabditis elegans Genotype: njIs9 IV. Description: njIs9 [glr-3p::snb-1::venus + ofm-1::GFP] IV. Mutagen: UV/TMP to integrate Outcrossed: x10 Made by: I. Mori Received: 01/16/07 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK732 Species: Caenorhabditis elegans Genotype: maco-1(nj21) I. Description: Reference: Miyara A, et al. PLoS Genet. 2011 May;7(5):e1001384. Mutagen: EMS Outcrossed: x10 Made by: Miyara & Mori Received: 06/10/13 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK734 Species: Caenorhabditis elegans Genotype: maco-1(nj34) I. Description: Reference: Miyara A, et al. PLoS Genet. 2011 May;7(5):e1001384. Mutagen: EMS Outcrossed: x10 Made by: Miyara & Mori Received: 06/10/13 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK777 Species: Caenorhabditis elegans Genotype: egl-8(nj77) V. Description: Reference: Kimata T, et al. Genetics. 2012 Jun;191(2):509-21. Mutagen: EMS Outcrossed: x5 Made by: Kimata & Mori Received: 06/10/13 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK800 Species: Caenorhabditis elegans Genotype: gcy-8(oy44) IV. Description: Nearly normal thermotaxis behavior. Mutagen: EMS Outcrossed: x10 Made by: Satterlee/Sengupta Received: 09/10/08 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IK850 Species: Caenorhabditis elegans Genotype: aho-3(nj15) I. Description: Reference: Nishio N, et al. Genes Cells. 2012 May;17(5):365-86. Mutagen: EMS Outcrossed: x10 Made by: Nishio & Mori Received: 06/10/13 Nagoya University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: IM222 Species: Caenorhabditis elegans Genotype: npr-1(ur89) X. Description: npr-1(ur89) has clumping, hyperactive, and bordering phenotypes. Mutagen: EMS Outcrossed: x6 Made by: Qun Wang Received: 05/09/02 Rutgers Robert Wood Johnson Medical School ----------------------------------------------------------------------------- Strain: IM324 Species: Caenorhabditis elegans Genotype: nid-1(ur41) V; edIs20 X. Description: edIs20 [F25B3.3::GFP + rol-6(su1006)]. Rollers. Pan-neuronal GFP expression. ur41 has longitudinal nerve defects. Mutagen: EMS Outcrossed: x5 Made by: Seonhe Kim Received: 05/30/01 Rutgers Robert Wood Johnson Medical School ----------------------------------------------------------------------------- Strain: IMN26 Species: Caenorhabditis elegans Genotype: dapk-1(gk219) I; glt-3(bz34) IV; nuIs5 V. Description: nuIs5 [glr-1::GFP + glr-1::G(alpha)s(Q227L) V + lin-15(+)] V. dapk-1(gk219) decreases neurodegeneration. Reference: Del Rosario J, et al. BMC Neurosci. 2015 Apr 23;16:25. Mutagen: Outcrossed: x Made by: John Del Rosario Received: 12/17/15 City College, The City University of New York, NY, USA ----------------------------------------------------------------------------- Strain: IMN27 Species: Caenorhabditis elegans Genotype: dapk-1(gk219) I; glt-3(bz34) IV. Description: Reference: Del Rosario J, et al. BMC Neurosci. 2015 Apr 23;16:25. Mutagen: Outcrossed: x Made by: John Del Rosario Received: 12/15/15 City College, The City University of New York, NY, USA ----------------------------------------------------------------------------- Strain: IMN28 Species: Caenorhabditis elegans Genotype: dapk-1(gk219) I; nuIs5 V. Description: nuIs5 [glr-1::GFP + glr-1::G(alpha)s(Q227L) V + lin-15(+)] V. Reference: Del Rosario J, et al. BMC Neurosci. 2015 Apr 23;16:25. Mutagen: Outcrossed: x Made by: John Del Rosario Received: 12/15/15 City College, The City University of New York, NY, USA ----------------------------------------------------------------------------- Strain: IMN30 Species: Caenorhabditis elegans Genotype: pinn-1(tm2235) II; glt-3(bz34) IV; nuIs5 V. Description: nuIs5 [glr-1::GFP + glr-1::G(alpha)s(Q227L) V + lin-15(+)] V. pinn-1(tm2235) increases neurodegeneration. Reference: Del Rosario J, et al. BMC Neurosci. 2015 Apr 23;16:25. Mutagen: Outcrossed: x Made by: John Del Rosario Received: 12/17/15 City College, The City University of New York, NY, USA ----------------------------------------------------------------------------- Strain: IMN31 Species: Caenorhabditis elegans Genotype: grp-1 (tm1956) III; glt-3(bz34) IV; nuIs5 V. Description: nuIs5 [glr-1::GFP + glr-1::G(alpha)s(Q227L) V + lin-15(+)] V. Reference: Tehrani N, et al. (2014) PLoS One 9(11):e113060. Mutagen: Outcrossed: x Made by: Received: 04/17/15 City College, The City University of New York, NY, USA ----------------------------------------------------------------------------- Strain: IMN32 Species: Caenorhabditis elegans Genotype: arf-1.2(ok796) III; glt-3(bz34) IV; nuIs5 V. Description: nuIs5 [glr-1::GFP + glr-1::G(alpha)s(Q227L) V + lin-15(+)] V. Reference: Tehrani N, et al. (2014) PLoS One 9(11):e113060. Mutagen: Outcrossed: x Made by: Received: 04/17/15 City College, The City University of New York, NY, USA ----------------------------------------------------------------------------- Strain: IMN33 Species: Caenorhabditis elegans Genotype: gqIs25 I; glt-3(bz34) IV; nuIs5 V. Description: nuIs5 [glr-1::GFP + glr-1::G(alpha)s(Q227L) V + lin-15(+)] V. Reference: Tehrani N, et al. (2014) PLoS One 9(11):e113060. Mutagen: Outcrossed: x Made by: Received: 04/17/15 City College, The City University of New York, NY, USA ----------------------------------------------------------------------------- Strain: IMN34 Species: Caenorhabditis elegans Genotype: ced-4(n1162) dpy-17(e164) III; glt-3(bz34) IV; nuIs5 V. Description: nuIs5 [glr-1::GFP + glr-1::G(alpha)s(Q227L) V + lin-15(+)] V. Reference: Tehrani N, et al. (2014) PLoS One 9(11):e113060. Mutagen: Outcrossed: x Made by: Received: 04/17/15 City College, The City University of New York, NY, USA ----------------------------------------------------------------------------- Strain: iOP50 Species: E. coli Genotype: OP50 E. coli, rnc14::(delta)Tn10, lacz(gamma)A::T7pol camFRT Description: RNAi-capable OP50 strain. Tetracycline & Chloramphenicol resistant. Uracil auxotroph. E. coli B. Biosafety Level: BSL-1. Standard OP50 (CGC) was rendered RNAi competent through two modifications by phage transduction. The OP50 RNase III (rnc) allele was replaced with the deletion allele (rnc14::ΔTn10) found in HT115(DE3), and a genomically encoded IPTG-inducible T7 RNA polymerase (laczγA::T7pol camFRT) was introduced. Reference: Neve IAA, et al. G3 (Bethesda). 2019 Nov 11. pii: g3.400741.2019. Mutagen: Phage transduction (2x) Outcrossed: x0 Made by: Jessica Sowa, Isaiah Neve Received: 12/02/19 Baylor College of Medicine, Houston, TX ----------------------------------------------------------------------------- Strain: IP1001 Species: Caenorhabditis elegans Genotype: nhr-6(lg6001)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Homozygous nhr-6(lg6001) mutants have defective spermatheca development causing low brood size, abnormally shaped eggs, and occasional Egl. Heterozygotes are WT and segregate WT, semi-sterile WT, and Sterile Dpys. Mutagen: Outcrossed: x6 Made by: Chris Gissendanner Received: 08/28/09 The University of Louisiana-Monroe, Monroe LA, USA ----------------------------------------------------------------------------- Strain: IT1187 Species: C. elegans Genotype: unc-119(ed3) III; kpIs100. Description: kpIs100 [pie-1p::Ub(G76V)::GFP::H2B::drp-1 3' UTR + unc-119(+)]. Expresses Ubiquitin::GFP fusion protein in the germ line. The last amino acid of ubiquitin is mutated to valine (G76V), which prevents the cleavage of ubiquitin from GFP by the deubiqutinating enzymes and renders the GFP constitutively targeted for degradation by the proteasome. The strain can be used to assay proteasome activity in the germ line. Reference: Kumar GA & Subramaniam K. Development. 2018 Mar 29;145(7):dev163949. PMID: 29540500 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Ganga Anil Kumar Received: 11/28/22 Indian Institute of Technology, Madras, India ----------------------------------------------------------------------------- Strain: IT540 Species: Caenorhabditis elegans Genotype: gap-3(kp1) I; puf-8(zh17) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are wild type and segregate wild type heterozygotes, paralyzed DpyUncs, and Uncs with germline tumors. Pick WT and check segregation of progeny to maintain. Reference: Vaid S, et al. Development. 2013 Apr;140(8):1645-54. Mutagen: EMS Outcrossed: x6 Made by: M. Ariz & S. Vaid Received: 01/22/15 Indian Institute of Technology, Madras, India ----------------------------------------------------------------------------- Strain: IU10 Species: Caenorhabditis elegans Genotype: daf-16(mgDf47) I; rrf-3(pk1426) II. Description: Mutagen: Outcrossed: x Made by: Received: 07/22/05 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: IU7 Species: Caenorhabditis elegans Genotype: rrf-3(pk1426) II; sir-2.1(ok434) IV. Description: Mutagen: Outcrossed: x Made by: Received: 07/22/05 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: IW412 Species: Caenorhabditis elegans Genotype: tax-2(gk117937) I. Description: Improved survival at 37C. Dauer constitutive and longer lifespan at 28C. Reference: Hwang HY, et al. Front Genet. 2020 Oct 6;11:566948. doi: 10.3389/fgene.2020.566948. PMID: 33133151 Mutagen: Outcrossed: x6 Made by: Ho-Yon Hwang Received: 12/28/21 Johns Hopkins University ----------------------------------------------------------------------------- Strain: IW465 Species: Caenorhabditis elegans Genotype: tax-2(iw80) I. Description: Improvement in thermotolerance at 37C, though weaker than in null mutants. Longer lifespan than to tax-2(gk117937) null mutant. Higher frequency of dauer formation and survival at 28C than tax-2(gk117937) null mutant. Reference: Hwang HY, et al. Front Genet. 2020 Oct 6;11:566948. doi: 10.3389/fgene.2020.566948. PMID: 33133151 Mutagen: EMS Outcrossed: x9 Made by: Ho-Yon Hwang Received: 12/28/21 Johns Hopkins University ----------------------------------------------------------------------------- Strain: IX3447 Species: Caenorhabditis elegans Genotype: kyIs140 I; vyEx1462. Description: kyIs140 [str-2::GFP + lin-15(+)] I. vyEx1462 (die-1(fosmid)::Venus + odr-1::DsRed + ofm-1::DsRed). Pick animals with DsRed+ coelomocytes to maintain. Animals with vyEx1462 show 2AWC(OFF) phenotype. Extrachromosomal array rescues die-1(w34). Mutagen: Outcrossed: x Made by: Yi-Wen Hsieh Received: 12/16/13 University of Illinois at Chicago, Chicago, IL ----------------------------------------------------------------------------- Strain: IX3597 Species: Caenorhabditis elegans Genotype: kyIs140 I; vyEx1510. Description: kyIs140 [str-2::GFP + lin-15(+)] I. vyEx1510 (ceh-36p::die-1(cDNA) + odr-1::DsRed + ofm-1::DsRed). Pick animals with DsRed+ coelomocytes to maintain. Animals with vyEx1510 show 2AWC(OFF) phenotype. Mutagen: Outcrossed: x Made by: Yi-Wen Hsieh Received: 12/16/13 University of Illinois at Chicago, Chicago, IL ----------------------------------------------------------------------------- Strain: IX4506 Species: C elegans Genotype: mls-2(vy248[mNG::mls-2]) X. Description: mNG tag inserted into endogenous mls-2 locus after the start codon using CRISPR/Cas9 engineering, producing a translational mNG::MLS-2 reporter protein. Derived by SEC excision of mls-2(vy247[mNG::SEC::mls-2 knock-in]) in parental strain. mNG::MLS-2 is detected in the nucleus of a few cells in embryos, and localizes to the nucleus of a subset of head cells and the M mesoblast in larvae and adults. Reference: Xiong R., et al. (2022). mNG-tagged mls-2 knock-in alleles in C. elegans. microPublication Biology. 10.17912/micropub.biology.000529. Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Rui Xiong Received: 06/22/22 University of Illinois at Chicago, Chicago, IL ----------------------------------------------------------------------------- Strain: IZ1458 Species: C. elegans Genotype: ufIs126 V. Description: ufIs126 [flp-13p::acr-12::GFP + lgc-11::mCherry] V. ACR-12::GFP expression labels postsynaptic iAChRs in DD motor neurons. References: Philbrook A, et al. eLife. 2018 Jul 24;7:e35692. doi: 10.7554/eLife.35692. PMID: 30039797. Oliver D, et al. PLoS Genet. 2022 Jan 28;18(1):e1010016. doi: 10.1371/journal.pgen.1010016. PMID: 35089924. Alexander K, et al. bioRxiv 2022.10.21.512874; doi: https://doi.org/10.1101/2022.10.21.512874. Mutagen: Integrated by X-rays Outcrossed: x8 Made by: Alison Philbrook Received: 01/06/23 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: JA1194 Species: Caenorhabditis elegans Genotype: egl-27(we3) II. Description: egl-27(we3) animals have variable body morphology defects with cold sensitive embryonic/L1 lethality (5% die at 22C, 90% at 15C). Maternal effect vab-7(ed6) enhancer; semi-lethal with vab-7(ed6). Mutagen: EMS Outcrossed: x3 Made by: F. Solari Received: 11/08/99 University of Cambridge, Cambridge, UK ----------------------------------------------------------------------------- Strain: JA1334 Species: Caenorhabditis elegans Genotype: unc-119(e2498) III; weIs11. Description: weIs11[unc-119(+) + TAC-1::GFP]. Mutagen: Bombardment Outcrossed: x Made by: R Andrews/N Le Bot Received: 02/18/09 University of Cambridge, Cambridge, UK ----------------------------------------------------------------------------- Strain: JA1354 Species: Caenorhabditis elegans Genotype: unc-119(e2498) III; weIs12. Description: weIs12[unc-119(+) + pie-1p::GFP::csnk-1]. Mutagen: Bombardment Outcrossed: x Made by: Costanza Panbianco Received: 02/18/09 University of Cambridge, Cambridge, UK ----------------------------------------------------------------------------- Strain: JA1403 Species: Caenorhabditis elegans Genotype: unc-119(e2498) III; weIs15. Description: weIs15 [pie-1p::GFP::eea-1(FYVEx2) + unc-119(+)]. Phenotypically wild type. Early endosomes in germline and embryos are GFP+. GFP is fused to the tandem FYVE domains of eea-1/T10G3.5, under control of the pie-1 promoter. Grows at any temp, but has bright GFP at 25C. Mutagen: Microparticle Bombardment Outcrossed: x0 Made by: Robert Andrews Received: 10/03/07 University of Cambridge, Cambridge, UK ----------------------------------------------------------------------------- Strain: JAR16 Species: C. elegans Genotype: rps-6(rns6[rps-6::mCherry]) I. Description: mCherry tag inserted at 3' end of endogenous rps-6 locus. Reduced thermotolence at 35C compared to N2 controls. Reference: Somers H, et al. Cell Reports Methods. (2022) Apr 25;2(4):100203 PMID: 35497499 Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Jarod Rollins Received: 06/06/23 Mt Desert Island Bio Lab, Salisbury Cove, ME ----------------------------------------------------------------------------- Strain: JAR50 Species: C. elegans Genotype: rack-1(rns8[rack-1::mScarlet]] IV. Description: mScarlet tag inserted into endogenous rack-1 locus. Ubiquitous mScarlet expression. Mutagen: Outcrossed: x0 Made by: Jarod Rollins Received: 06/06/23 Mt Desert Island Bio Lab, Salisbury Cove, ME ----------------------------------------------------------------------------- Strain: JBL1 Species: Caenorhabditis elegans Genotype: tonSi1 II; unc-119(ed3) III. Description: tonSi1 [mex-5p::Dendra2::his-66::tbb-2 3'UTR + Cbr-unc-119(+)] II. MosSCI insertion into ttTi5605 II. Maintain at 20-25C. Reference: Bolkova J and Lanctot C (2015) Int J Dev Biol, in press. Mutagen: MosSCI Outcrossed: x2 Made by: Jitka Bolkova Received: 03/31/16 Charles University, Prague, Czech Republic ----------------------------------------------------------------------------- Strain: JBL2 Species: Caenorhabditis elegans Genotype: tonSi1 II; unc-119(ed3) III; ddIs6 V. Description: tonSi1 [mex-5p::Dendra2::his-66::tbb-2 3'UTR + Cbr-unc-119(+)] II. MosSCI insertion into ttTi5605 II. ddIs6 [pie-1p::GFP::tbg-1 + unc-119(+)] V. Maintain at 20-25C. Derived by crossing JBL1 and TH27. Reference: Bolkova J and Lanctot C (2015) Int J Dev Biol, in press. Mutagen: MosSCI Outcrossed: x Made by: Jitka Bolkova Received: 03/31/16 Charles University, Prague, Czech Republic ----------------------------------------------------------------------------- Strain: JBL3 Species: Caenorhabditis elegans Genotype: tonSi1 II; unc-119(ed3) III; axIs1522. Description: tonSi1 [mex-5p::Dendra2::his-66::tbb-2 3'UTR + Cbr-unc-119(+)] II. MosSCI insertion into ttTi5605 II. axIs1522 [pie-1p::GFP::pgl-1::pgl-1 3'UTR + unc-119(+)]. Maintain at 20-25C. Derived by crossing JBL1 and JH2108. Reference: Bolkova J and Lanctot C (2015) Int J Dev Biol, in press. Mutagen: MosSCI Outcrossed: x Made by: Jitka Bolkova Received: 03/31/16 Charles University, Prague, Czech Republic ----------------------------------------------------------------------------- Strain: JC1225 Species: Caenorhabditis elegans Genotype: mrp-1(ut153) X. Description: Synthetic Daf at 15C when in an unc-31(e169) background. Mutagen: EMS Outcrossed: x4 Made by: T Yabe & N Suzuki Received: 01/17/06 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: JC153 Species: Caenorhabditis elegans Genotype: unc-104(ut60)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUncs and unc-104(ut60) animals which are L1 lethal coilers. Mutagen: EMS Outcrossed: x Made by: Isao Katsura Received: 06/03/00 University of Utah, Salt Lake City ----------------------------------------------------------------------------- Strain: JC182 Species: Caenorhabditis elegans Genotype: unc-52(ut111)/unc-52(e444) II. Description: Heterozygotes are slow (weak) Uncs and segregate paralyzed Uncs and early larval lethals. Dead larvae (ut111 homozygotes) are short, 2-fold and have an irregular shape. Mutagen: ut111: spontaneous in RW7097 Outcrossed: x Made by: Kazunori Kondo Received: 03/13/95 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: JC1970 Species: Caenorhabditis elegans Genotype: tbx-2(ut180) III. Description: Synthetic dauer-constitutive with unc-31(e169) or unc-3(e151). Defective in adaptation to benzaldehyde, isoamyl alcohol, and butanone. Normal in adaptation to diacetyl and 2-methylpyrazine. Normal in chemotaxis to volatile and water-soluble chemicals. Mutagen: EMS Outcrossed: x3 Made by: Norio Suzuki Received: 08/19/04 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: JC1971 Species: Caenorhabditis elegans Genotype: tbx-2(ut192) III. Description: Synthetic dauer-constitutive with unc-31(e169) or unc-3(e151). Defective in adaptation to benzaldehyde, isoamyl alcohol, and butanone. Normal in adaptation to diacetyl and 2-methylpyrazine. Normal in chemotaxis to volatile and water-soluble chemicals. Mutagen: EMS Outcrossed: x3 Made by: Norio Suzuki Received: 08/19/04 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: JC201 Species: Caenorhabditis elegans Genotype: hch-1(ut110) X. Description: Delayed hatching from egg shell. QL and descendant cells migrate forward instead of backward (incomplete penetrance). Tc1 insertion mutant. Mutagen: Spontaneous in mut-6. Outcrossed: x2 Made by: Kazunori Kondo Received: 03/17/97 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: JC2154 Species: Caenorhabditis elegans Genotype: hen-1(tm501) X. Description: Hesitation in crossing an aversive ion. Defective in behavioral plasticity after conditioned with NaCl and starvation or with temperature and starvation. Mutagen: UV/TMP Outcrossed: x6 Made by: S. Mitani Received: 08/09/02 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: JC2159 Species: Caenorhabditis elegans Genotype: bbs-8(ut306) V. Description: Butanone enhancement abnormal. Dyf. Weak Dpy. Allelic to bbs-8(nx77). Mutagen: EMS Outcrossed: x3 Made by: Ichiro Torayama Received: 04/27/07 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: JC2199 Species: Caenorhabditis elegans Genotype: sdf-9(ut163) V. Description: Forms dauer larvae in the unc-31(e169) background at 25C. Forms dauer-like larvae at 25C on NGM plates without cholesterol. Mutagen: EMS Outcrossed: x6 Made by: K Ohkura & N Suzuki Received: 09/11/03 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: JC2202 Species: Caenorhabditis elegans Genotype: sdf-9(ut187) V. Description: Forms dauer larvae in the unc-31(e169) background at 25C. Forms dauer-like larvae at 25C on NGM plates without cholesterol. Mutagen: EMS Outcrossed: x6 Made by: K Ohkura & N Suzuki Received: 09/11/03 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: JC2209 Species: Caenorhabditis elegans Genotype: olrn-1(ut305) X. Description: Butanone enhancement abnormal. Abnormal chemotaxis to x1/1000 butanone. 2AWC-OFF (no expression of str-2::GFP in either of the two AWC neurons. Allelic to nsy-6(ky626). Mutagen: EMS Outcrossed: x5 Made by: Ichiro Torayama Received: 04/27/07 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: JC2319 Species: Caenorhabditis elegans Genotype: ghi-1(ut293) II. Description: Mutagen: UV/TMP Outcrossed: x5 Made by: Akane Oishi Received: 08/25/09 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: JC2361 Species: Caenorhabditis elegans Genotype: flr-6(ut285) V. Description: Mutagen: EMS Outcrossed: x4 Made by: Isao Kutsura Received: 08/25/09 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: JC2362 Species: Caenorhabditis elegans Genotype: flr-7(ut286) V. Description: Mutagen: EMS Outcrossed: x4 Made by: Isao Kutsura Received: 08/25/09 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: JC2749 Species: Caenorhabditis elegans Genotype: flr-4(ut3) X; utEx33. Description: utEx33 [flr-1p::GFP + rol-6(su1006)]. Pick Rollers to maintain. GFP is localized exclusively to intestinal nuclei because of the nuclear localization signal and lack of FLR-1 membrane spanning domains. Reference: Take-uchi et al.: Proc Natl Acd Sci USA. 1998 Sep 29;95(20):11775-80, Take-uchi et al.: Mol Biol Cell. 2005 Mar;16(3):1355-65, Kobayashi et al.: Genes to Cells 2011 May;16(5):565-75. Mutagen: Outcrossed: x Made by: Yuri Kobayashi Received: 06/01/11 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: JC328 Species: Caenorhabditis elegans Genotype: flr-5(ut73) V. Description: Partially resistant to 400ug/ml NaF. Suppressor of the slow-growing phenotype of mutations in flr-1, flr-3, and flr-4. Mutagen: EMS Outcrossed: x Made by: Isao Katsura Received: 05/21/93 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: JC49 Species: Caenorhabditis elegans Genotype: gpla-1(ut5) V. Description: Partially resistant to 400ug/ml NaF. Weakly Dpy. (Not determined if this is due to ut5 or a closely linked mutation.) Suppressor of the slow-growing phenotype of mutations in flr-1, flr-3, and flr-4. gpla-1 formerly known as flr-2. Mutagen: EMS Outcrossed: x Made by: Isoa Katsura Received: 05/21/93 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: JC51 Species: Caenorhabditis elegans Genotype: flr-4(ut7) X. Description: Resistant to 400ug/ml NaF. Slow growth. Small brood size. Small and thin. Mutagen: EMS Outcrossed: x Made by: Isao Katsura Received: 05/21/93 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: JC53 Species: Caenorhabditis elegans Genotype: flr-3(ut9) IV. Description: Resistant to 400ug/ml NaF. Slow growth. Small brood size. Small and thin. Mutagen: mut-5 Outcrossed: x Made by: Isao Katsura Received: 05/21/93 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: JC55 Species: Caenorhabditis elegans Genotype: flr-1(ut11) X. Description: Resistant to 400ug/ml NaF. Slow growth. Small brood size. Small and thin. Mutagen: mut-6 Outcrossed: x Made by: Isao Katsura Received: 05/21/93 National Institute of Genetics, Mishima, Japan ----------------------------------------------------------------------------- Strain: JCB418 Species: C. elegans Genotype: Y67H2A.2(bet63) IV. Description: Homozygous viable. Deletion of 2572 bp in parental strain N2. Left flanking sequence: atctatttttttaaggccgaac; Right flanking sequence: tattggcagcaagcgttgcgaa. sgRNA #1: ccatacgttgttgtggagtt; sgRNA #2: tgtgaagcggaaaaccctat. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Bettinger lab Received: 12/17/24 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: JCB419 Species: C. elegans Genotype: Y76A2B.4(bet65) III. Description: Homozygous viable. Deletion of 1579 bp in parental strain N2. Left flanking sequence: gcaaaaaaaaacataccaga; Right flanking sequence: cgtggtttcaggccattacg. sgRNA #1: cctcactgatgatcgtcatc; sgRNA #2: aaaggttcagcattcacacg. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Bettinger lab Received: 12/17/24 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: JCB426 Species: C. elegans Genotype: chil-11(bet66) IV. Description: Homozygous viable. Deletion of 2532 bp in parental strain N2. Left flanking sequence: agtcaattcggaactccatgt; Right flanking sequence: tctacggtttaaacaactcctc. sgRNA #1: aacgggatctgttcatcaca; sgRNA #2: agtgtgaaacgcaacgtcta. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Bettinger lab Received: 12/17/24 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: JCB434 Species: C. elegans Genotype: K04C2.8(bet68) III. Description: Homozygous viable. Deletion of 796 bp in parental strain N2, with insertion of 13 nucleotides(tcaacaaaatgcc) at break. Left flanking sequence: taatatcctccggaccgata; Right flanking sequence: gtcctgactgataatcatcaac. sgRNA #1: tgtgtagtataaacgattat; sgRNA #2: cgggtcacgagtagagatgg. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Bettinger lab Received: 12/17/24 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: JCB435 Species: C. elegans Genotype: C14B1.9(bet70) III. Description: Homozygous viable. Deletion of 973 bp in parental strain N2. Left flanking sequence: aaactacggtaacacccatt; Right flanking sequence: tgacggatgcaatgacaaga. sgRNA #1: gagacctacacatgccaaat; sgRNA #2: tttggattaatgttacctga. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Bettinger lab Received: 12/17/24 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: JCB455 Species: C. elegans Genotype: K02D10.3(bet75) III. Description: Homozygous viable. Deletion of 247 bp in parental strain N2.Deletion appears to have occurred at the sgRNA #2 cut site. Left flanking sequence: tttataggaatttcaggaat; Right flanking sequence: ttccgtcaccttccgtcaaa. sgRNA #1: aaatgataagaagccaaagc; sgRNA #2: tttgtgtttatgacgagctc. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Bettinger lab Received: 12/17/24 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: JCB456 Species: C. elegans Genotype: algn-12(bet74) V/nT1[qls51] (IV;V). Description: Homozygous sterile. Balanced by nT1[qIs51]. Deletion of 3471 bp in parental strain N2. Left flanking sequence: tgatcactcacagttccctgg; Right flanking sequence: gaatggatatgatgatgtatat. sgRNA #1: atgttcgtggaacgacacca; sgRNA #2: aggataaactctctcttgaa. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Bettinger lab Received: 12/17/24 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: JCB458 Species: C. elegans Genotype: T19C4.5(bet77) V. Description: Homozygous viable. Deletion of 1581 bp in parental strain N2. Also contains a SNP (A->T) in right flanking sequence (uppercase text). Left flanking sequence: ctactcatgatataccttct; Right flanking sequence: ccggTgctctcttgttgtct. sgRNA #1: gttttcacatgggcaagaga; sgRNA #2: atttcaattccatttcccac. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Bettinger lab Received: 12/17/24 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: JCB459 Species: C. elegans Genotype: C18E9.4(bet80) II. Description: Homozygous viable. Deletion of 492 bp in parental strain N2. Left flanking sequence: agccgtttaagatcccaaac; Right flanking sequence: ggatggtcatcattagaatt. sgRNA #1: ttgctgtagattgagtagtt; sgRNA #2: cacggaaccacctcatggga. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Bettinger lab Received: 12/17/24 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: JCB461 Species: C. elegans Genotype: Y116F11B.14(bet83) V. Description: Homozygous viable. Deletion of 1493 bp in parental strain N2. Left flanking sequence: attaatttttgaatttcctaca; Right flanking sequence: tgacgggctaatattgaatta. sgRNA #1: attacactataataatgtgt; sgRNA #2: aaacgacaaactcattatga. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Bettinger lab Received: 12/17/24 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: JCB487 Species: C. elegans Genotype: K02D10.1(bet88) III. Description: Homozygous viable. Deletion of 2796 bp in parental strain N2. Left flanking sequence: tatgaactttaagaccaact; Right flanking sequence: ggatgggatgcaactgttgc. sgRNA #1: actcatactataagttcagt; sgRNA #2: ctacttgggcaaagccagga. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Bettinger lab Received: 12/17/24 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: JCM1 Species: Caenorhabditis elegans Genotype: hyl-2(gnv1) X. Description: gnv1 was found in strain AT10. hyl-2(+):CATCAT: aagcgcagtgatttctggcaaatgcttgttcatcattttatcaccctcgcacttattgg tgtttca; hyl-2(gnv1): CAATAT: aagcgcagtgatttctggcaaatgcttgttcaatattttatcaccctcgcacttattgg tgtttca. Anoxia sensitive. Heat-shock (36 deg Celcius) sensitive. Mutagen: Outcrossed: x4 Made by: Vincent Menuz Received: 06/04/09 University of Geneva, Geneva, Switzerland ----------------------------------------------------------------------------- Strain: JCP152 Species: Caenorhabditis elegans Genotype: dpy-11(e224) ccz-1(t2129) V; jcpEx2. Description: jcpEx2 [ced-1p::F58G11.6(genomic)::YFP::let-858 3'UTR + unc-119(+) + myo-2::GFP]. Maintain by picking GFP+. Individuals that lost the array produce only arrested embryos with spindle orientation defects, accumulate vesicles, and problems engulfing apoptotic corpses. Reference: Nieto C, et al. J Cell Sci. 2010 Jun 15;123(Pt 12):2001-7. Mutagen: EMS Outcrossed: x>3 Made by: J. Cabello Received: 10/20/10 Center for Biomedical Research of La Rioja, Logrono, Spain ----------------------------------------------------------------------------- Strain: JCP169 Species: Caenorhabditis elegans Genotype: dpy-11(e224) ccz-1(t2129) V; jcpEx3. Description: jcpEx3 [ccz-1p::ccz-1(genomic)::YFP::let-858 3'UTR + unc-119(+) + pha-1(+)]. Array rescues lethality. Individuals that lost the array produce only arrested embryos with spindle orientation defects, accumulate vesicles, and problems engulfing apoptotic corpses. Reference: Nieto C, et al. J Cell Sci. 2010 Jun 15;123(Pt 12):2001-7. Mutagen: EMS Outcrossed: x>3 Made by: J. Cabello Received: 10/20/10 Center for Biomedical Research of La Rioja, Logrono, Spain ----------------------------------------------------------------------------- Strain: JCP294 Species: C. elegans Genotype: ints-6(t1903) IV. Description: Temperature-sensitive. Grows well at 15C; embryonic lethal at 25C. ints-6 previously known as dic-1. Reference: Gómez-Orte E, et al. PLoS Genet. 2019 Feb 26;15(2):e1007981. Mutagen: EMS Outcrossed: x10 Made by: Ralf Schnabel, Juan Cabello Received: 04/29/19 Center for Biomedical Research of La Rioja, Logrono, Spain ----------------------------------------------------------------------------- Strain: JCP301 Species: C. elegans Genotype: jcpSi3 II; unc-119(ed3) III. Description: jcpSi3 [ins-37p::ins-37::eGFP::ins-37 3'UTR + unc-119(+)] II. Superficially wild-type. Reference: Gómez-Orte E, et al. PLoS Genet. 2019 Feb 26;15(2):e1007981. Mutagen: MosSCI Outcrossed: x0 Made by: Knudra Nemametrix Received: 04/29/19 Center for Biomedical Research of La Rioja, Logrono, Spain ----------------------------------------------------------------------------- Strain: JCP341 Species: C.elegans Genotype: jcpSi10 II; unc-119(ed3) III. Description: jcpSi10 [ints-6p::ints-6::3xFLAG::eGFP::ints-6 3'UTR + unc-119(+)] II. Superficially wild-type. ints-6 previously known as dic-1. Reference: Gómez-Orte E, et al. PLoS Genet. 2019 Feb 26;15(2):e1007981. Mutagen: MosSCI Outcrossed: x0 Made by: Knudra Nemametrix Received: 04/29/19 Center for Biomedical Research of La Rioja, Logrono, Spain ----------------------------------------------------------------------------- Strain: JCP343 Species: C. elegans Genotype: jcpSi12 II; unc-119(ed3) III. Description: jcpSi12 [W04G5.8p::W04G5.8::eGFP::W04G5.8 3'UTR + unc-119(+)] II. Superficially wild-type. Reference: Gómez-Orte E, et al. PLoS Genet. 2019 Feb 26;15(2):e1007981. Mutagen: MosSCI Outcrossed: x0 Made by: Knudra Nemametrix Received: 04/29/19 Center for Biomedical Research of La Rioja, Logrono, Spain ----------------------------------------------------------------------------- Strain: JCP378 Species: C. elegans Genotype: jcpSi19 II; unc-119(ed3) III. Description: jcpSi19 [eft-3p::ints-6::3xFLAG::eGFP::ints-6 3'UTR + unc-119(+)] II. Superficially wild-type. ints-6 previously known as dic-1. Reference: Gómez-Orte E, et al. PLoS Genet. 2019 Feb 26;15(2):e1007981. Mutagen: MosSCI Outcrossed: x0 Made by: Knudra Nemametrix Received: 04/29/19 Center for Biomedical Research of La Rioja, Logrono, Spain ----------------------------------------------------------------------------- Strain: JCP383 Species: C. elegans Genotype: jcpSi10 II; ints-6(tm1615) IV. Description: jcpSi10 [ints-6p::ints-6::3xFLAG::eGFP::ints-6 3'UTR + unc-119(+)] II. Superficially wild-type. ints-6 previously known as dic-1. Reference: Gómez-Orte E, et al. PLoS Genet. 2019 Feb 26;15(2):e1007981. Mutagen: Outcrossed: x0 Made by: Eva Gomez-Orte Received: 04/29/19 Center for Biomedical Research of La Rioja, Logrono, Spain ----------------------------------------------------------------------------- Strain: JCP387 Species: C. elegans Genotype: jcpSi24 II; unc-119(ed3) III. Description: jcpSi24 [H27M09.5p::H27M09.5::3xFLAG::eGFP::H27M09.5 3'UTR + unc-119(+)] II. Superficially wild-type. Reference: Gómez-Orte E, et al. PLoS Genet. 2019 Feb 26;15(2):e1007981. Mutagen: MosSCI Outcrossed: x0 Made by: Knudra Nemametrix Received: 04/29/19 Center for Biomedical Research of La Rioja, Logrono, Spain ----------------------------------------------------------------------------- Strain: JCP394 Species: C. elegans Genotype: jcpSi31 II; unc-119(ed3) III. Description: jcpSi31 [Y75B8A.23p::Y75B8A.23::3xFLAG::eGFP::Y75B8A.2 3'UTR + unc-119(+)] II. Superficially wild-type. Reference: Gómez-Orte E, et al. PLoS Genet. 2019 Feb 26;15(2):e1007981. Mutagen: MosSCI Outcrossed: x0 Made by: Knudra Nemametrix Received: 04/29/19 Center for Biomedical Research of La Rioja, Logrono, Spain ----------------------------------------------------------------------------- Strain: JCP405 Species: C. elegans Genotype: jcpSi37 II; unc-119(ed3) III. Description: jcpSi37 [F08H9.3p::F08H9.3::3xFLAG::eGFP::F08H9.3 3'UTR + unc-119(+)] II. Superficially wild-type. Reference: Gómez-Orte E, et al. PLoS Genet. 2019 Feb 26;15(2):e1007981. Mutagen: MosSCI Outcrossed: x0 Made by: Knudra Nemametrix Received: 04/29/19 Center for Biomedical Research of La Rioja, Logrono, Spain ----------------------------------------------------------------------------- Strain: JCP462 Species: C. elegans Genotype: ints-6(jcp1[ints-6::3xFLAG]) IV. Description: 3xFLAG tag inserted into the endogenous ints-6 locus. Superficially wild-type. ints-6 previously known as dic-1. Reference: Gómez-Orte E, et al. PLoS Genet. 2019 Feb 26;15(2):e1007981. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Knudra Nemametrix Received: 04/29/19 Center for Biomedical Research of La Rioja, Logrono, Spain ----------------------------------------------------------------------------- Strain: JCP495 Species: Caenorhabditis elegans Genotype: nxf-1(t2160) V. Description: Temperature-sensitive. Maintain at 15-20C. 100% embryonic lethal at 25C. Pharynx unattached (Pun) and morphogenic defects. Reference: Zheleva A, et al. PLoS Genet. 2019 Sep 16;15(9):e1008338. Mutagen: EMS Outcrossed: x5 Made by: Angelina Zheleva, Juan Cabello, Ralf Schnabel Received: 11/07/19 Center for Biomedical Research of La Rioja, Logrono, Spain ----------------------------------------------------------------------------- Strain: JCP519 Species: Caenorhabditis elegans Genotype: nxf-1(t2160) V; jcpEx6. Description: jcpEx6 [nxf-1p::nxf-1::3xFLAG::eGFP::nxf-1UTR]. jcpEx6 transgene rescues nxf-1(t2160) embryonic lethality at 25C. Temperature-sensitive; maintain at 25C to retain the array. nxf-1(t2160) mutants are 100% embryonic lethal at 25C. Reference: Zheleva A, et al. PLoS Genet. 2019 Sep 16;15(9):e1008338. Mutagen: Outcrossed: xx Made by: Angelina Zheleva Received: 11/08/19 Center for Biomedical Research of La Rioja, Logrono, Spain ----------------------------------------------------------------------------- Strain: JCP53 Species: Caenorhabditis elegans Genotype: dpy-11(e224) ccz-1(t2129) V/nT1 [qIs51] (IV;V). Description: Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ccz-1 homozygotes (produce only arrested embryos with spindle orientation defects, accumulate vesicles, and problems engulfing apoptotic corpses). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Nieto C, et al. J Cell Sci. 2010 Jun 15;123(Pt 12):2001-7. Mutagen: EMS Outcrossed: x>3 Made by: J. Cabello Received: 11/08/10 Center for Biomedical Research of La Rioja, Logrono, Spain ----------------------------------------------------------------------------- Strain: JD105 Species: Caenorhabditis elegans Genotype: avr-15(ad1051) V. Description: Starved; feeding defective; lacks M3 neurotransmission. From DA1051. DA1051 has bor-1 in the background. bor-1 has been crossed out in JD105. Mutagen: EMS Outcrossed: x4 Made by: Joe Dent Received: 05/29/07 McGill University, Montreal QC, Canada ----------------------------------------------------------------------------- Strain: JD204 Species: Caenorhabditis elegans Genotype: vuEx90. Description: vuEx90 [avr-14p::GFP]. Reference: Dent et al., 2000, Proc. Nat. Acad. Sci. 97(6): 2674-2679. Mutagen: Outcrossed: x0 Made by: K. Steger Received: 12/03/14 McGill University, Montreal QC, Canada ----------------------------------------------------------------------------- Strain: JD21 Species: Caenorhabditis elegans Genotype: cca-1(ad1650) X. Description: Slow pharyngeal pumping. Abnormal pharyngeal muscle depolarization. Mutagen: UV/TMP Outcrossed: x7 Made by: K. Steger Received: 10/13/05 McGill University, Montreal QC, Canada ----------------------------------------------------------------------------- Strain: JD217 Species: Caenorhabditis elegans Genotype: gar-3(vu78) V. Description: Mutagen: Outcrossed: x Made by: Kate Steger Received: 09/09/04 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: JD255 Species: Caenorhabditis elegans Genotype: gar-3(lg1201) V; gar-1(ad1676) X. Description: Pharyngeal pumping anomalies. Arecoline resistance. Locomotion variant. Reference: Steger KA & Avery L. Genetics. 2004 Jun;167(2):633-43. Mutagen: Outcrossed: x0 Made by: Kate Steger Received: 01/22/18 McGill University, Montreal QC, Canada ----------------------------------------------------------------------------- Strain: JD269 Species: Caenorhabditis elegans Genotype: gar-2(by124) III; gar-3(lg1201) V; gar-1(ad1676) X. Description: Locomotion and feeding defects. Triple mutant lacks all muscarinic signalling. Reference: Steger KA & Avery L. Genetics. 2004 Jun;167(2):633-43. Mutagen: Outcrossed: x0 Made by: Kate Steger Received: 01/22/18 McGill University, Montreal QC, Canada ----------------------------------------------------------------------------- Strain: JD31 Species: Caenorhabditis elegans Genotype: glc-4(ok212) II. Description: C27H5.8. No obvious phenotype. URL: http://www.celeganskoconsortium.omrf.org. Mutagen: UV/TMP Outcrossed: x2 Made by: Gary Moulder Received: 04/02/02 McGill University, Montreal QC, Canada ----------------------------------------------------------------------------- Strain: JD596 Species: Caenorhabditis elegans Genotype: avr-15(vu227) V. Description: Slow pumping. vu227 is the allele that is actually present in strains DA1316 and DA1370, not ad1051. For those working with DA1316 and DA1370 it may be useful to have an avr-15(vu227) single mutant for comparison. Reference: Dent et al., 2000, Proc. Nat. Acad. Sci. 97(6): 2674-2679. Mutagen: EMS Outcrossed: x2 Made by: Joseph Dent Received: 12/03/14 McGill University, Montreal QC, Canada ----------------------------------------------------------------------------- Strain: JD600 Species: Caenorhabditis elegans Genotype: avr-15(ad1051) glc-1(pk54) V. Description: Slow pumping. This strain is a replacement for the strain DA1370 whose genotype is actually avr-15(vu227) glc-1(pk54) V. Reference: Dent et al., 2000, Proc. Nat. Acad. Sci. 97(6): 2674-2679. Mutagen: EMS Outcrossed: x2 Made by: Joseph Dent Received: 12/03/14 McGill University, Montreal QC, Canada ----------------------------------------------------------------------------- Strain: JD608 Species: Caenorhabditis elegans Genotype: avr-14(ad1302) I; avr-15(ad1051) glc-1(pk54) V. Description: Slow pumping. High reversal frequency. Ivermectin resistant. This strain is a replacement for the strain DA1316 currently in the CGC stock and whose genotype is actually avr-14(ad1305) I; avr-15(vu227) glc-1(pk54) V. Reference: Dent et al., 2000, Proc. Nat. Acad. Sci. 97(6): 2674-2679. Mutagen: EMS Outcrossed: x2 Made by: Joseph Dent Received: 12/03/14 McGill University, Montreal QC, Canada ----------------------------------------------------------------------------- Strain: JD740 Species: Caenorhabditis elegans Genotype: avr-14(ad1302) I; avr-15(ad1051) V. Description: Modest ivermectin resistance. Mild feeding defect. Lacks M3 neurotransmission. Reference: Dent JA, et al. Proc Natl Acad Sci U S A. 2000 Mar 14;97(6):2674-9. Mutagen: Outcrossed: x2x Made by: Joe Dent Received: 01/22/18 McGill University, Montreal QC, Canada ----------------------------------------------------------------------------- Strain: JDW10 Species: C. elegans Genotype: wrdSi3 II. Description: wrdSi3 [sun-1p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (II:0.77). Germline and early embryo-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in germline and early embryo nuclei. Reference: Ashley GE, et al. Genetics. 2021 Mar 31;217(3):iyab006. doi: 10.1093/genetics/iyab006. PMID: 33677541 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Matt Ragle Received: 07/31/20 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW182 Species: C. elegans Genotype: bmdSi15 lmn-1(wrd39[lmn-1::1xGFP11]) I. Description: bmdSi15 [loxN + eef-1A.1p::GFP(1-10)::unc-54 3′ UTR + let-858 terminator + myo-2p::mCherry::3xHA::tbb-2 3′ UTR + loxP] I. bmdSi15 is a CRISPR-based integration into the ttTi4348 site (I:-5.32). Somatic expression of sfGFP(1-10) driven by the eft-3 promoter. GFP11 tag inserted into endogenous lmn-1 locus via CRISPR/Cas9 insertion into parental strain DQM104. Reference: Gregory EF, et al. MicroPubl Biol. 2023 Dec 13:2023:10.17912/micropub.biology.001022. doi: 10.17912/micropub.biology.001022. eCollection 2023. PMID: 38152058. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 03/07/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW220 Species: C. elegans Genotype: wrdSi18 I. Description: wrdSi18 [^SEC^mex-5p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (I:-5.32). Germline and early embryo-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in germline and early embryo nuclei. Self-excising cassette with Rol and HygroR markers still in strain to facilitate crosses. Heat-shock to remove SEC as described in Dickinson et al. 2015. Reference: Ashley GE, et al. Genetics. 2021 Mar 31;217(3):iyab006. doi: 10.1093/genetics/iyab006. PMID: 33677541 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Matt Ragle Received: 07/31/20 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW221 Species: C. elegans Genotype: wrdSi50 I. Description: wrdSi50 [mex-5p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (I:-5.32). Germline and early embryo-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in germline and early embryo nuclei. Reference: Ashley GE, et al. Genetics. 2021 Mar 31;217(3):iyab006. doi: 10.1093/genetics/iyab006. PMID: 33677541 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Matt Ragle Received: 07/31/20 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW222 Species: C. elegans Genotype: wrdSi8 II. Description: wrdSi8 [^SEC^mex-5p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (II:0.77). Germline and early embryo-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in germline and early embryo nuclei. Self-excising cassette with Rol and HygroR markers still in strain to facilitate crosses. Heat-shock to remove SEC as described in Dickinson et al. 2015. Reference: Ashley GE, et al. Genetics. 2021 Mar 31;217(3):iyab006. doi: 10.1093/genetics/iyab006. PMID: 33677541 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Matt Ragle Received: 07/31/20 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW223 Species: C. elegans Genotype: wrdSi51 II. Description: wrdSi51 [mex-5p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (II:0.77). Germline and early embryo-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in germline and early embryo nuclei. Reference: Ashley GE, et al. Genetics. 2021 Mar 31;217(3):iyab006. doi: 10.1093/genetics/iyab006. PMID: 33677541 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Matt Ragle Received: 07/31/20 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW224 Species: C. elegans Genotype: wrdSi22 I. Description: wrdSi22 [^SEC^eft-3p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (I:-5.32). SEC spotaneously excises at a high frequency so non-rollers will appear. Pick Rollers to maintain. Pan-somatic expression of TIR1 co-factor for AID, and expression of AID-tagged blue protein in somatic nuclei. Self-excising cassette with Rol and HygroR markers still in strain to facilitate crosses. Heat-shock to remove SEC as described in Dickinson et al. 2015. Reference: Ashley GE, et al. Genetics. 2021 Mar 31;217(3):iyab006. doi: 10.1093/genetics/iyab006. PMID: 33677541 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Matt Ragle Received: 07/31/20 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW225 Species: C. elegans Genotype: wrdSi23 I. Description: wrdSi23 [eft-3p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (I:-5.32). Pan-somatic expression of TIR1 co-factor for AID, and expression of AID-tagged blue protein in somatic nuclei. Reference: Ashley GE, et al. Genetics. 2021 Mar 31;217(3):iyab006. doi: 10.1093/genetics/iyab006. PMID: 33677541 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Matt Ragle Received: 07/31/20 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW227 Species: C. elegans Genotype: wrdSi45 II. Description: wrdSi45 [dpy-7p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (II:0.77). Hypodermal-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in hypodermal nuclei. Reference: Ashley GE, et al. Genetics. 2021 Mar 31;217(3):iyab006. doi: 10.1093/genetics/iyab006. PMID: 33677541 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Matt Ragle Received: 07/31/20 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW229 Species: C. elegans Genotype: wrdSi47 I. Description: wrdSi47 [dpy-7p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (I:-5.32). Hypodermal-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in hypodermal nuclei. Reference: Ashley GE, et al. Genetics. 2021 Mar 31;217(3):iyab006. doi: 10.1093/genetics/iyab006. PMID: 33677541 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Matt Ragle Received: 07/31/20 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW231 Species: C. elegans Genotype: wrdSi44 II. Description: wrdSi44 [SCMp*::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (II:0.77). Seam cell-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in seam cell nuclei. Some expression in hypodermal cells. Reference: Ashley GE, et al. Genetics. 2021 Mar 31;217(3):iyab006. doi: 10.1093/genetics/iyab006. PMID: 33677541 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Matt Ragle Received: 07/31/20 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW233 Species: C. elegans Genotype: wrdSi46 I. Description: wrdSi46 [SCMp*::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (I:-5.32). Seam cell-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in seam cell nuclei. Some expression in hypodermal cells. Reference: Ashley GE, et al. Genetics. 2021 Mar 31;217(3):iyab006. doi: 10.1093/genetics/iyab006. PMID: 33677541 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Matt Ragle Received: 07/31/20 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW29 Species: C. elegans Genotype: nhr-23(wrd8[nhr-23::GFP::AID*::3xFLAG]) I. Description: GFP::AID*::3xFLAG tag inserted at the C-terminus of the endogenous nhr-23 locus by CRISPR. Allele obtained using the self-excising casstte, following Dickinson et al, 2015 method. Reference: Ragle JM, et al. Development. 2020 Nov 27;147(22):dev193862. doi: 10.1242/dev.193862. PMID: 33060131. Mutagen: CRISPR Outcrossed: x3 Made by: Jordan Ward Received: 03/15/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW313 Species: C. elegans Genotype: jsSi1579; wrdSi58 II. Description: wrdSi58 [eft-3p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (II:0.77). Pan-somatic expression of TIR1 co-factor for AID, and expression of AID-tagged blue protein in somatic nuclei. jsSi1579 is an RMCE landing pad inserted at a sgRNA site 45 bp from the ttTi5605 insertion site. It contains an rpl-28p::GFP reporter flanked by FRT and FRT3 sites and a loxP site (for more details about landing pads, see Nonet, 2020.Genetics or visit https://sites.wustl.edu/nonetlab/rmce/). Reference: Vo AA, et al. MicroPubl Biol. 2021 Aug 3;2021:10.17912/micropub.biology.000425. doi: 10.17912/micropub.biology.000425. eCollection 2021. PMID: 34355140 Mutagen: Outcrossed: x2 Made by: Jordan D Ward Received: 08/11/21 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW324 Species: C. elegans Genotype: jsSi1579; wrdSi57 II. Description: wrdSi57 [^SEC^eft-3p:TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (II:0.77). Pick Rollers to maintain. Pan-somatic expression of TIR1 co-factor for AID, and expression of AID-tagged blue protein in somatic nuclei. Self-excising cassette with Rol and HygroR markers still in strain to facilitate crosses. Heat-shock to remove SEC as described in Dickinson et al. 2015. jsSi1579 is an RMCE landing pad inserted at a sgRNA site 45 bp from the ttTi5605 insertion site. It contains an rpl-28p::GFP reporter flanked by FRT and FRT3 sites and a loxP site (for more details about landing pads, see Nonet, 2020.Genetics or visit https://sites.wustl.edu/nonetlab/rmce/). Reference: Vo AA, et al. MicroPubl Biol. 2021 Aug 3;2021:10.17912/micropub.biology.000425. doi: 10.17912/micropub.biology.000425. eCollection 2021. PMID: 34355140 Mutagen: Outcrossed: x2 Made by: Jordan D Ward Received: 08/11/21 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW389 Species: C. elegans Genotype: bli-1(wrd84[bli-1::linker::mNeonGreen::3xFLAG(internal)::linker]) II. Description: Internal mNeonGreen::3xFLAG tags with linker sequences inserted into endogenous bli-1 locus. Superficially wild-type. Reference: Johnson LC, et al. Development 2023; dev.201085. doi: https://doi.org/10.1242/dev.201085. Mutagen: CRISPR/Cas Outcrossed: x0 Made by: Matt Ragle Received: 06/08/23 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW390 Species: C. elegans Genotype: bli-2(wrd85[bli-2::mNG::3xFLAG]) II. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous bli-2 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 09/23/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW436 Species: C. elegans Genotype: nas-37(wrd106[nas-37:::mNG::3xFLAG]) X. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous nas-37 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 09/05/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW460 Species: C. elegans Genotype: noah-1(wrd119[noah-1::linker::mNeonGreen(dpiRNA)::3xFLAG(internal)::linker]) I. Description: Internal mNeonGreen(dpiRNA)::3xFLAG tags with linker sequences inserted into endogenous noah-1 locus. mNeonGreen(dpiRNA) is optimized to remove all piRNA sites. Superficially wild-type. Reference: Johnson LC, et al. Development 2023; dev.201085. doi: https://doi.org/10.1242/dev.201085. Mutagen: CRIPSR/Cas9 Outcrossed: x0 Made by: Matt Ragle Received: 06/08/23 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW478 Species: C. elegans Genotype: cpz-1(wrd128[cpz-1::mNG::3xFLAG::linker]) I. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted internally in exon 4 of the endogenous cpz-1 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 07/26/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW628 Species: C. elegans Genotype: nhr-85(wrd29[nhr-85::GFP::AID*::3xFLAG]) I. Description: GFP::AID*::3xFLAG tag inserted at the C-terminus of the endogenous nhr-85 locus using CRISPR self-excising casstte (Dickinson et al, 2015 method). Reference: Myles KM, et al. MicroPubl Biol. 2023 Oct 18:2023:10.17912/micropub.biology.000993. doi: 10.17912/micropub.biology.000993. eCollection 2023. PMID: 37927911. Mutagen: CRISPR Outcrossed: x4 Made by: Jordan Ward Received: 03/15/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW650 Species: C. elegans Genotype: dpy-4(wrd228[dpy-4::mNG::3xFLAG]) IV. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous dpy-4 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 07/26/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW651 Species: C. elegans Genotype: dpy-14(wrd229[dpy-14::mNG::3xFLAG]) I. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous dpy-14 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 07/26/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW652 Species: C. elegans Genotype: rol-8(wrd230[rol-8mNG::3xFLAG]) II. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous rol-8 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 07/26/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW653 Species: C. elegans Genotype: sqt-2(wrd231[sqt-2::mNG::3xFLAG]) II. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous sqt-2 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 07/26/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW654 Species: C. elegans Genotype: ram-2(wrd232[ram-2::mNG::3xFLAG]) II. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous ram-2 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 07/26/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW655 Species: C. elegans Genotype: cut-2(wrd233[cut-2::mNG::3xFLAG]) V. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous cut-2 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 07/26/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW656 Species: C. elegans Genotype: npa-1(wrd234[npa-1::mNG::3xFLAG]) V. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous npa-1 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 12/03/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW684 Species: C. elegans Genotype: nhr-23(wrd33[nhr-23::30aa linker::mScarlet::SEC::3XMyc]) I. Description: mScarlet::3xMyc tag inserted at the C-terminus of the endogenous nhr-23 locus by CRISPR. A flexible 30 amino acid linker is between the nhr-23 coding sequence and mScarlet. Allele obtained using the self-excising casstte, following Dickinson et al, 2015 method. Reference: Myles KM, et al. MicroPubl Biol. Oct 2:2023:10.17912/micropub.biology.000996. doi: 10.17912/micropub.biology.000996. eCollection 2023. PMID: 37854098. Mutagen: CRISPR Outcrossed: x4 Made by: Jordan Ward Received: 03/07/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW699 Species: C. elegans Genotype: col-14(wrd271[col-14::mNG::3xFLAG]) IV. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous col-14 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 07/26/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW708 Species: C. elegans Genotype: nas-37(wrd106 wrd251[nas-37::mScarlet::2xOLLAS]) X. Description: linker::mScarlet::2xOLLAS::linker inserted at the C-terminus of the endogenous nas-37 locus by CRISPR using a Cas9 RNP. Allele obtained by replacing a modular mNeonGreen::3xFLAG cassette from wrd106. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 09/23/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW733 Species: C. elegans Genotype: mlt-8(wrd278[mlt-8::mNG::3xFLAG]) II. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous mlt-8 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 10/04/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW734 Species: C. elegans Genotype: col-12(wrd279[col-12::mNG::3xFLAG]) V. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous col-12 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 09/05/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW735 Species: C. elegans Genotype: col-41(wrd280[col-41::mNG::3xFLAG]) X. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted 6 bp before the stop codon in the endogenous col-41 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 09/05/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW736 Species: C. elegans Genotype: clec-180(wrd281[clec-180::mNG::3xFLAG]) IV. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous clec-180 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 10/04/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW737 Species: C. elegans Genotype: mlt-10(wrd282[mlt-10::mNG::3xFLAG]) II. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous mlt-10 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 09/05/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW739 Species: C. elegans Genotype: mlt-9(wrd284[mlt-9::mNG::3xFLAG]) X. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous mlt-9 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 01/29/25 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW740 Species: C. elegans Genotype: acn-1(wrd285[acn-1::mNG::3xFLAG]) X. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted internally in the first exon of the endogenous acn-1 locus by CRISPR. Will produce a linker::mNG::3xFLAG::linker fusion after the 32nd amino acid. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 09/05/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW741 Species: C. elegans Genotype: qua-1(wrd286[qua-1::mNG::3xFLAG]) II. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous qua-1 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 01/29/25 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW742 Species: C. elegans Genotype: cpg-7(wrd287[cpg-7:::mNG::3xFLAG]) II. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous cpg-7 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 10/04/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW764 Species: C. elegans Genotype: col-125(wrd300[col-125::mNG::3xFLAG]) IV. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous col-125 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 10/31/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW765 Species: C. elegans Genotype: col-103(wrd301[col-103::mNG::3xFLAG]) IV. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous col-103 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 09/23/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW766 Species: C. elegans Genotype: piit-1(wrd302[piit-1::mNG::3xFLAG]) V. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous piit-1 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 09/05/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW767 Species: C. elegans Genotype: ctsa-1.1(wrd303[ctsa-1.1::mNG::3xFLAG]) II. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous ctsa-1.1 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 01/29/25 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW769 Species: C. elegans Genotype: lon-8(wrd305[lon-8::mNG::3xFLAG]) V. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous lon-8 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 09/23/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW780 Species: C. elegans Genotype: col-125(wrd300 wrd312[col-125::mScarlet::2xOLLAS]) IV. Description: linker::mScarlet::2xOLLAS::linker inserted at the C-terminus of the endogenous col-125 locus by CRISPR using a Cas9 RNP. Allele obtained by replacing a modular mNeonGreen::3xFLAG cassette from wrd300. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 10/04/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW781 Species: C. elegans Genotype: col-103(wrd301 wrd313[col-103::mScarlet::2xOLLAS]) IV. Description: linker::mScarlet::2xOLLAS::linker inserted at the C-terminus of the endogenous col-103 locus by CRISPR using a Cas9 RNP. Allele obtained by replacing a modular mNeonGreen::3xFLAG cassette from wrd301. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 10/04/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW782 Species: C. elegans Genotype: piit-1(wrd302 wrd314[piit-1::mScarlet::2xOLLAS]) V. Description: linker::mScarlet::2xOLLAS::linker inserted at the C-terminus of the endogenous piit-1 locus by CRISPR using a Cas9 RNP. Allele obtained by replacing a modular mNeonGreen::3xFLAG cassette from wrd302. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 09/23/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW783 Species: C. elegans Genotype: ctsa-1.1(wrd303 wrd315[ctsa-1.1::mScarlet::2xOLLAS]) II. Description: linker::mScarlet::2xOLLAS::linker inserted at the C-terminus of the endogenous ctsa-1.1 locus by CRISPR using a Cas9 RNP. Allele obtained by replacing a modular mNeonGreen::3xFLAG cassette from wrd303. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 10/04/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW785 Species: C. elegans Genotype: lon-8(wrd305 wrd317[lon-8::mScarlet::2xOLLAS]) V. Description: linker::mScarlet::2xOLLAS::linker inserted at the C-terminus of the endogenous lon-8 locus by CRISPR using a Cas9 RNP. Allele obtained by replacing a modular mNeonGreen::3xFLAG cassette from wrd305. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 10/04/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW786 Species: C. elegans Genotype: srap-1(wrd318[srap-1::mNG::3xFLAG]) II. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted in the first exon after the signal sequenceof the endogenous srap-1 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 02/20/25 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW788 Species: C. elegans Genotype: col-166(wrd319[col-166::mNG::3xFLAG]) X. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted internally to produce a translational fusion after amino acid 120 in the endogenous col-166 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 10/31/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW789 Species: C. elegans Genotype: lrp-1(wrd320[lrp-1::mNG::3xFLAG]) I. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted internally in the last exon of the endogenous lrp-1 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: CRISPR Outcrossed: x0 Made by: Jordan Ward Received: 02/20/25 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW794 Species: C. elegans Genotype: dpy-14(wrd229 wrd325[dpy-4::mScarlet::2xOLLAS) I. Description: mScarlet::2xOLLAS tag inserted at the C-terminus of the endogenous dpy-14 locus by CRISPR. Generated by replacing mNG::3xFLAG with mScarlet::2xOLLAS in parental strain JDW651. Mutagen: Outcrossed: x0 Made by: Jordan Ward Received: 09/23/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW802 Species: Caenorhabditis elegans Genotype: dao-2(wrd328[dao-2::mNG::3xFLAG]) II. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous dao-2 locus by CRISPR using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: Outcrossed: x0 Made by: Jordan Ward Received: 10/31/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW818 Species: Caenorhabditis elegans Genotype: dpy-13(syb3318(wrd337[dpy-13::30x linker::mScarlet(dpi)::10x linker]) IV. Description: 30x linker::mScarlet(dpi)::10x linker tag inserted into the C-terminus of the endogenous dpy-13 locus by CRISPR using a Cas9 RNP. Derived by modification of syb3318[dpy-13::mNG] in parental strain PHX3318, replacing mNeonGreen with the mScarlet and linkers. Mutagen: Outcrossed: x0 Made by: Jordan Ward Received: 10/31/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW827 Species: C. elegans Genotype: col-118(wrd343[col-118::mNG::3xFLAG]) IV. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous col-118 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jordan Ward Received: 12/03/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW828 Species: Caenorhabditis elegans Genotype: col-13(wrd344[col-13::mNG::3xFLAG]) V. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous col-13 locus by CRISPR. Allele obtained using Cas9 RNP. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jordan Ward Received: 12/03/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW834 Species: Caenorhabditis elegans Genotype: unc-119(kst33) col-97(wrd346[col-97::internal mNG::3xFLAG + unc-119(+)]) III. Description: Superficially wild type. Modular linker::mNeonGreen::3xFLAG::linker tag inserted in the endogenous col-97 locus by CRISPR to produce a translational fusion inserted internally near the C-terminus (after amino acid 288). Allele obtained using plasmid-based unc-119 selection. Injection of a Cre recombinase plasmid failed to excise the loxP-flanked unc-119(+) cassette for unknown reasons. The insert was verified to be correct through Sanger sequencing. Knock-in is linked to the temperature-sensitive unc-119(kst33) allele. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jordan Ward Received: 03/12/25 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW850 Species: Caenorhabditis elegans Genotype: col-75(wrd349[col-75::mNG::3xFLAG]) II. Description: Modular linker::mNeonGreen::3xFLAG::linker tag inserted at the C-terminus of the endogenous col-75 locus by CRISPR. Allele obtained using plasmid-based unc-119 selection in a temperature-sensitive unc-119(kst33) III background The unc-119(+) cassette was removed through Cre-mediated excision and the unc-119(kst33) allele was removed by outcrossing. Cassette design allows for re-editing of locus with common crRNAs/sgRNAs. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Jordan Ward Received: 02/20/25 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JDW92 Species: C. elegans Genotype: wrdSi19 nhr-23(kry61[nhr-23::AID*::TEV::3xFLAG]) I; him-5(e1490) V. Description: wrdSi19 [mex-5p::TIR1:F2A:mTagBFP2:tbb-2 3'UTR] (I:-5.32). Strain allows germline-specific depletion of NHR-23::AID*LLTEV::3xFLAG using the auxin-inducible degron system. wrdSi19 was made by crossing parental strain KRY87 to JDW83 [wrdSi10 (mex-5p::TIR1:F2A:mTagBFP2:tbb-2 3′UTR+SEC, I:-5.32); him5(e1490) V] and using heatshock to remove the SEC. Reference: Ragle JM, et al. Development. 2020 Nov 27;147(22):dev193862. doi: 10.1242/dev.193862. PMID: 33060131. Mutagen: CRISPR Outcrossed: x Made by: Jordan Ward Received: 03/07/24 University of California, Santa Cruz, CA ----------------------------------------------------------------------------- Strain: JE32 Species: Caenorhabditis elegans Genotype: pat-3(st564) III; mwEx32. Description: mwEx32 [pat-3(YYFF) + sur-5::GFP]. Pick GFP+ to maintain. mwEx32 carries a mutant form of pat-3 gene with tyrosine (Y) to phenylalanine (F) mutations; rescues pat-3 null allele. Abnormal DTC migration. Reference: Lee M, et al. J Biol Chem. 2001 Sep 28;276(39):36404-10. Mutagen: none Outcrossed: x0 Made by: Myeongwoo Lee Received: 07/17/19 Baylor University, Waco, TX ----------------------------------------------------------------------------- Strain: JE443 Species: Caenorhabditis elegans Genotype: pat-3(st564) III; mwEx443. Description: mwEx443 [pat-3(+) + sur-5::GFP]. Pick GFP+ to maintain. mwEx443 contains wild type contains pat-3 genomic DNA, rescues pat-3 null allele. Reference: Lee M, et al. J Biol Chem. 2001 Sep 28;276(39):36404-10. Mutagen: none Outcrossed: x0 Made by: Myeongwoo Lee Received: 07/17/19 Baylor University, Waco, TX ----------------------------------------------------------------------------- Strain: JEK1001 Species: Caenorhabditis elegans Genotype: ddx-15 (tm4014) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP tm4014 homozygotes (sterile). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. The tm4014 allele was provided by the National BioResource Project (NBRP, for C. elegans). Mutagen: Outcrossed: x0 Made by: Jonathan Karpel Received: 02/14/14 Southern Utah University, Cedar City, UT ----------------------------------------------------------------------------- Strain: JEK114 Species: Caenorhabditis elegans Genotype: ddx-15(kar1[gfp::ddx-15]) III. Description: GFP inserted at N-terminus of endogenous ddx-15 locus. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jonathan Karpel Received: 01/15/19 Southern Utah U ----------------------------------------------------------------------------- Strain: JEL1000 Species: C. elegans Genotype: hsr-9(xoe17) I. Description: Superfically wild-type. hsr-9(xoe17) was generated by incorporating a stop-in cassette early in the coding region using the co-CRISPR method (Paix et al. 2015). The hsr-9 repair template (gattttgcctcttaaataaaatttcagCAAAAAACCGAGGGGAGACTTGCAATAGGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAGCTAGCTCTCGGATCATCTTGCAAACATGCTTATTGCTGgtaggtattgcaacc) and guide RNA (AGGGGAGACTTGCAATATCT) were injected into N2 and the resulting progeny were analyzed by PCR using TGAAATTAAGGTGGTCACTCGAAG and GTTGTTGTGGGGAGGCTGAA. Reference: Hariri S, et al. (2023). 53bp1 mutation enhances brca1 and bard1 embryonic lethality in C. elegans. microPublication Biology. 10.17912/micropub.biology.000934. PMID: 37581122. Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Sara Hariri Received: 09/08/23 University of California Davis ----------------------------------------------------------------------------- Strain: JEL1016 Species: C. elegans Genotype: hsr-9(xoe17) I; brc-1(xoe4) III. Description: Emb. Reference: Hariri S, et al. (2023). 53bp1 mutation enhances brca1 and bard1 embryonic lethality in C. elegans. microPublication Biology. 10.17912/micropub.biology.000934. PMID: 37581122. Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Sara Hariri Received: 09/08/23 University of California Davis ----------------------------------------------------------------------------- Strain: JEL1134 Species: C. elegans Genotype: polq-1(xoe51) III. Description: Superfically wild-type. polq-1(xoe51) was generated by incorporating a stop-in cassette early in the coding region using the co-CRISPR method (Paix et al. 2015). The polq-1 repair template (AGAGAATTCTCTGAAGATCCATTAATATTGCTTACCGAAGGGGAAGTTTGTCCAGAGCAGAGGTGACTAAGTGATAAGCTAGCAGAGTTTTCGCCGCAATTCTCAGACTTTGGTAATGATTTC) and guide RNA (ATTGCGGCGAAAACTCTCTT) were injected into N2 and the resulting progeny were analyzed by PCR using ATAGGCAAATGGCTGGACGG and TCAAAGCAGTCTTCTCGGCA. Reference: Hariri S, et al. (2023). 53bp1 mutation enhances brca1 and bard1 embryonic lethality in C. elegans. microPublication Biology. 10.17912/micropub.biology.000934. PMID: 37581122. Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Sara Hariri Received: 09/08/23 University of California Davis ----------------------------------------------------------------------------- Strain: JEL1142 Species: C. elegans Genotype: hsr-9(xoe17) I; brc-1(xoe4) polq-1(xoe51) III. Description: Emb. Reference: Hariri S, et al. (2023). 53bp1 mutation enhances brca1 and bard1 embryonic lethality in C. elegans. microPublication Biology. 10.17912/micropub.biology.000934. PMID: 37581122. Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Sara Hariri Received: 09/08/23 University of California Davis ----------------------------------------------------------------------------- Strain: JEL1162 Species: C. elegans Genotype: brd-1(xoe18) III. Description: Weak Emb and Him. N2 parental background. Reference: Li Q, et al. PLoS Genet. 2023 Jan 30;19(1):e1010457. doi: 10.1371/journal.pgen.1010457. PMID: 36716349. Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Sara Hariri Received: 09/08/23 University of California Davis ----------------------------------------------------------------------------- Strain: JEL1197 Species: Caenorhabditis elegans Genotype: wrdSi3 II; dpy-27(xoe41[dpy-27::AID::myc]) III; him-8(me4) IV. Description: wrdSi3 [sun-1p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (II:0.77). Endogenous dpy-27 locus tagged with AID element and myc to create a conditional allele using the auxin-inducible degron (AID). In absence of auxin, ~40% of worms are male due to him-8(me4) mutation. In the presence of auxin, ~100% of worms are male (hermaphrodite-lethal due to degradation of AID-tagged DPY-27). Reference: Li Q, et al. Inducible degradation of dosage compensation protein DPY-27 facilitates isolation of Caenorhabditis elegans males for molecular and biochemical analyses. bioRxiv 2022.01.27.478040; doi: https://doi.org/10.1101/2022.01.27.478040. Mutagen: CRISPR/Cas9 homologous recombination Outcrossed: x4 Made by: Arshdeep Kaur Received: 03/10/22 University of California Davis ----------------------------------------------------------------------------- Strain: JEL1319 Species: C. elegans Genotype: hsr-9(xoe17) I; brd-1(xoe18) III. Description: Emb. Reference: Hariri S, et al. (2023). 53bp1 mutation enhances brca1 and bard1 embryonic lethality in C. elegans. microPublication Biology. 10.17912/micropub.biology.000934. PMID: 37581122. Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: JoAnne Engebrecht Received: 09/08/23 University of California Davis ----------------------------------------------------------------------------- Strain: JEL446 Species: Caenorhabditis briggsae Genotype: Cbr-met-2(xoe1) III. Description: Derived from C. briggsae strain AF16. Reference: Larson BJ, et al. Genetics. 2016 Jun 8. pii: genetics.116.191130. Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Braden Larson Received: 07/20/16 University of California Davis ----------------------------------------------------------------------------- Strain: JEL447 Species: Caenorhabditis briggsae Genotype: Cbr-met-2(xoe2) III. Description: Inappropriate targeting of H3K9me2 and H3K9me3. Derived from C. briggsae strain AF16. Reference: Larson BJ, et al. Genetics. 2016 Jun 8. pii: genetics.116.191130. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Braden Larson Received: 07/20/16 University of California Davis ----------------------------------------------------------------------------- Strain: JEL730 Species: C. elegans Genotype: brc-1(xoe4) III. Description: Weak Emb and Him. Reference: Li Q, et al. PLoS Genet. 2023 Jan 30;19(1):e1010457. doi: 10.1371/journal.pgen.1010457. PMID: 36716349. Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Qianyan Li Received: 09/08/23 University of California Davis ----------------------------------------------------------------------------- Strain: JF85 Species: Caenorhabditis elegans Genotype: mtEx60. Description: mtEx60 [numr-1p::GFP + rol-6(su1006)]. Maintain under normal conditions by picking rollers. Reference: Tvermoes B, et al. (2010) J Cell Sci 123:2124-34. Mutagen: Outcrossed: x Made by: Jonathan Freedman Received: 09/30/10 University of Louisville School of Medicine, Louisville, KY ----------------------------------------------------------------------------- Strain: JF88 Species: Caenorhabditis elegans Genotype: mtEx63. Description: mtEx63 [numr-1p::numr-1::GFP + rol-6(su1006)]. Maintain under normal conditions by picking rollers. Reference: Tvermoes B, et al. (2010) J Cell Sci 123:2124-34. Mutagen: Outcrossed: x Made by: Jonathan Freedman Received: 09/30/10 University of Louisville School of Medicine, Louisville, KY ----------------------------------------------------------------------------- Strain: JG33 Species: Caenorhabditis elegans Genotype: vpIs9. Description: vpIs9 [unc-119(+) + elt-1::GFP] Expresses in hypodermal precursor cells in early embryo and seams cells in late embryos and in seam cells at a low level throughout post-embryonic development. Also in ventral cord and retropharyngeal ganglion in larvae and adults, and vulval muscles in adults. Mutagen: Outcrossed: x0 Made by: Received: 12/10/04 University of Calgary, Calgary, Alberta, Canada ----------------------------------------------------------------------------- Strain: JG5 Species: Caenorhabditis elegans Genotype: vpIs1. Description: vpIs1 [lin-15(+) + elt-3::GFP]. Mutagen: Outcrossed: x Made by: Received: 12/10/04 University of Calgary, Calgary, Alberta, Canada ----------------------------------------------------------------------------- Strain: JGG1 Species: Caenorhabditis elegans Genotype: Y53F4B.4(tm3898) II. Description: Superficially wild-type. Mutagen: TMP/UV Outcrossed: x6 Made by: Mitani & Grillari Received: 07/18/13 Medical University of Vienna ----------------------------------------------------------------------------- Strain: JH103 Species: Caenorhabditis elegans Genotype: axIs36 X. Description: axIs36 [pes-10::GFP + dpy-20(+)] X. Slightly dumpy. Mutagen: Outcrossed: x6 Made by: M. Wallenfang Received: 02/01/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH1270 Species: Caenorhabditis elegans Genotype: nos-1(gv5) II. Description: No visible phenotype except for reduced brood size. Synthetic sterile with nos-2(RNAi). 1176 bp deletion starting at aa 58 in nos-1 ORF and ending 414 bp past the end of the nos-1 ORF. Mutagen: EMS Outcrossed: x6 Made by: T Brodigan/M Krause Received: 01/24/00 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH1288 Species: Caenorhabditis elegans Genotype: cdk-7(ax224) I. Description: Temperature sensitive lethal. Maintain at 15C. Mutagen: Outcrossed: x2 Made by: M. Wallenfang Received: 07/31/02 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH1327 Species: Caenorhabditis elegans Genotype: axEx73. Description: axEx73 [pie-1p::pie-1::GFP + rol-6(su1006) + N2 genomic DNA]. pie-1::GFP is expressed in embryos and oocytes. This array may have integrated spontaneously since the strain segregates 100% Rollers. Mutagen: Outcrossed: x0 Made by: K. Reese/M. Dunn Received: 07/31/01 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH1448 Species: Caenorhabditis elegans Genotype: axEx1125. Description: axEx1125 [pie-1p::mex-5::GFP::pie-1 3’UTR + rol-6(su1006) + N2 genomic DNA]. Maintain at 25C. Pick Rollers to maintain. axEx1125 contains pKR2.04, a construct carrying MEX-5::GFP in vector pKR1.42; pKR1.42 uses the pie-1 promoter, enhancer, and 3′ UTR to drive maternal expression of GFP in embryos. Animals with the array are Rollers. Animals which have lost the array are WT. Mutagen: Outcrossed: x0 Made by: Kim Reese Received: 03/18/03 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH1463 Species: Caenorhabditis elegans Genotype: nos-2(ok230) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUncs and dead eggs. The nos-2(ok230) allele is due to deletion of bases 30999 to 33076 of cosmid ZK1127 (GenBank accession U58758) and also deletes a portion of the him-14 gene (him-14 is in an intron of nos-2). Mutagen: UV/TMP Outcrossed: x Made by: K Subramaniam Received: 01/07/04 Washington University School of Medicine, St. Louis MO, USA ----------------------------------------------------------------------------- Strain: JH1473 Species: Caenorhabditis elegans Genotype: itIs153; ojIs1. Description: itIs153 [pie-1p::par-2::GFP + rol-6(su1006) + N2 genomic DNA]. ojIs1 [pie-1p::GFP::tbb-2 + unc-119(+)]. Maintain at 25C. Constructed by crossing heterozygous ojIs1 males into KK866 (itIs153) hermaphrodites and selecting for double homozygosity of the arrays. itIs153 is an integrated derivitive of axEx1094. Mutagen: Outcrossed: x Made by: Geraldine Seydoux Received: 03/18/03 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH1521 Species: Caenorhabditis elegans Genotype: puf-8(ok302) II. Description: 100% Sterile at 25C. Him and 50% Emb at 20C. Maintain at 15C. Mutagen: UV/TMP Outcrossed: x3 Made by: K. Subramaniam Received: 08/19/03 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH1576 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1140. Description: axIs1140 [pie-1p::GFP::mbk-2 + unc-119(+)]. Mutagen: Bombardment Outcrossed: x0 Made by: J. Pellettieri Received: 01/14/04 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH1580 Species: Caenorhabditis elegans Genotype: unc-24(e1172) mbk-2(pk1427) IV/nT1 [let-?(m435)] (IV;V). Description: Heterozygotes are WT and segregate WT, Uncs which give only dead eggs, and dead eggs. mbk-2(pk1427) is a deletion that removes most of the mbk-2 locus. Mutagen: Outcrossed: x Made by: J. Pellettieri Received: 01/14/04 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH1964 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1427. Description: axIs1427 [pCG26/ LAP-tag DCP-1]. Reference: Gallo CM, et al. Dev Biol. 2008 Nov 1;323(1):76-87. Mutagen: Outcrossed: x Made by: Christopher Gallo Received: 11/19/10 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH1986 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1437. Description: axIs1437 [pCG31; LAP::lsm-1 + unc-119(+)]. GFP expression appears granular in somatic blastomeres at the 4-cell stage. Superficially wild-type. Reference: Dev Bio (2008) 323(1):76-87. Mutagen: Bombardment Outcrossed: x Made by: Christopher Gallo Received: 03/04/10 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH1999 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1448. Description: axIs1448 [pie-1p::GFP::H2B::nos-2(wt) 3'UTR + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Superficially wild-type. Reference: Merritt C, et al. Curr Biol. 2008 Oct 14;18(19):1476-82. Mutagen: Outcrossed: x Made by: Chris Meritt Received: 11/19/10 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2013 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1460. Description: axIs1460 [pie-1p::GFP::pal-1(genomic)::pal-1 3'UTR + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. pal-1 coding region and 3’ sequences were amplified from genomic DNA and cloned into pDONR201 to create ORF+3’ Entry Clones. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2015 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1462. Description: axIs1462 [pie-1p::GFP::pie-1 ORF::pie-1 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2017 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1464. Description: axIs1464 [pie-1p::GFP::pgl-3 ORF::pgl-3 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2019 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1466. Description: axIs1466 [pie-1p::GFP::mex-5 ORF::mex-5 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2024 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1471. Description: axIs1471 [pie-1p::GFP::fbf-1 ORF::fbf-1 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2054 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1492. Description: axIs1492 [pie-1p::GFP::tbb-2 ORF::tbb-2 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2060 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1498. Description: axIs1498 [pie-1p::GFP::gld-1 ORF::gld-1 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2078 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1504. Description: axIs1504 [pie-1p::LAP::mex-5::pie-1 3'UTR + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Reference: Gallo CM, et al. Science. 2010 Dec 17;330(6011):1685-9. Mutagen: Outcrossed: x Made by: Christopher Gallo Received: 05/09/11 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2080 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1506. Description: axIs1506 [pie-1p::GFP::exos-1::exos-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Mutagen: Outcrossed: x Made by: Christopher Gallo Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2099 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1486. Description: axIs1486 [pCG51; LAP::Y46G5A.13(tia-1.2) + unc-119(+)]. GFP expression appears in cytoplasmic granules in P1-Z2/Z3 lineages; nuclear & cytoplasmic in other blastomeres. Array is unstable and will silence after several generations. Superficially wild-type. Reference: Dev Bio (2008) 323(1):76-87. Mutagen: Bombardment Outcrossed: x Made by: Christopher Gallo Received: 03/04/10 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2100 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1487. Description: axIs1487 [pCG81; pie-1p::LAP::patr-1 + unc-119(+)]. GFP expression appears in cytoplasmic granules in P1-Z2/Z3 lineages; nuclear & cytoplasmic in other blastomeres. Maintain @ 25C; array is unstable and will silence after several generations. Superficially wild-type. Reference: Dev Bio (2008) 323(1):76-87. Mutagen: Bombardment Outcrossed: x Made by: Christopher Gallo Received: 06/01/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2107 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1720. Description: axIs1720 [pie-1p::GFP::pgl-1 ORF::pgl-1 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2108 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1522. Description: axIs1522 [pie-1p::GFP::pgl-1::pgl-1 3'UTR + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Transgene is prone to silencing -- maintain at 25C. Reference: Gallo CM, et al. Science. 2010 Dec 17;330(6011):1685-9. Mutagen: Outcrossed: x Made by: Christopher Gallo Received: 05/09/11 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2110 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1524. Description: axIs1524 [pie-1p::GFP::spe-38 ORF::spe-38 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2119 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1533. Description: axIs1533 [pie-1p::GFP::daz-1 ORF::daz-1 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2120 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1534. Description: axIs1534 [pie-1p::GFP::him-3 3'UTR + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C to retain transgene expression. GFP expressed in synapses. Weaker expression in oocytes and pachytene germline. Mutagen: Outcrossed: x Made by: Chris Meritt Received: 04/16/13 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2156 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1557. Description: axIs1557 [pie-1p::GFP::lip-1 ORF::lip-1 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2160 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1561. Description: axIs1561 [pie-1p::GFP::nos-3 ORF::nos-3 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2162 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1563. Description: axIs1563 [pie-1p::GFP::cep-1 ORF::cep-1 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2163 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1564. Description: axIs1564 [pie-1p::GFP::fog-2 ORF::fog-2 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2166 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1567. Description: axIs1567 [pie-1p::GFP::spn-4 ORF::spn-4 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Transgene is very unstable. Pick non-Unc, GFP+ worms to maintain. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2168 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1569. Description: axIs1569 [pie-1p::GFP::msp-142 ORF::msp-142 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2171 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1586. Description: axIs1586 [pie-1::LAP::glh-1::nos-2 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. GFP expression in P-granules of distal germline. Mutagen: Outcrossed: x Made by: K. Voronina Received: 03/20/13 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2184 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1595. Description: axIs1595 [pie-1p::GFP::npp-9(orf )::npp-9 3'UTR + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Reference: Voronina E & Seydoux G, Development. 2010 May;137(9):1441-50. Mutagen: Bombardment Outcrossed: x Made by: E Voronina Received: 11/19/10 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2194 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1571. Description: axIs1571 [pie-1p::GFP::par-5(orf)::par-5 3'UTR + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x Made by: Chris Meritt Received: 06/01/11 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2200 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1577. Description: axIs1577 [pie-1p::GFP::histone H2B::nos-3 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2207 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1584. Description: axIs1584 [pie-1p::GFP::histone H2B::fog-2 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2220 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1608. Description: axIs1608 [pie-1p::GFP::histone H2B::par-5 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2221 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1609. Description: axIs1609 [pie-1p::GFP::histone H2B::mex-5 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2223 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1611. Description: axIs1611 [pie-1p::GFP::histone H2B::daz-1 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2225 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1613. Description: axIs1613 [pie-1p::GFP::histone H2B::mes-2 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Transgene is slightly unstable. Pick non-Unc, GFP+ worms to maintain. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2228 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1616. Description: axIs1616 [pie-1p::GFP::histone H2B::spe-38 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Transgene is slightly unstable. Pick non-Unc, GFP+ worms to maintain. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2236 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1624. Description: axIs1624 [pie-1p::GFP::histone H2B::pal-1 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2252 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1640. Description: axIs1640 [pie-1p::GFP::histone H2B::glp-1 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2261 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1649. Description: axIs1649 [pie-1p::GFP::histone H2B::cye-1 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2267 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1651. Description: axIs1651 [pie-1p::GFP::histone H2B::lip-1 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2270 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1654. Description: axIs1654 [pie-1p::GFP::histone H2B::fbf-1 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2281 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1729. Description: axIs1729 [pie-1p::LAP tag::mCherry::nos-2 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Mutagen: Outcrossed: x Made by: E Voronina Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2296 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1663. Description: axIs1663 [pie-1p::GFP::histone H2B::fbf-2 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2297 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1664. Description: axIs1664 [pie-1p::GFP::histone H2B::tbb-2 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2311 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1668. Description: axIs1668 [pie-1p::GFP::histone H2B::spn-4 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2313 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1670. Description: axIs1670 [pie-1p::GFP::histone H2B:rme-2 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2316 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1673. Description: axIs1673 [pie-1p::GFP::histone H2B::msp-142 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2317 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1674. Description: axIs1674 [pie-1p::GFP::histone H2B::spe-11 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2320 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1677. Description: axIs1677 [pie-1p::GFP::histone H2B::pgl-1 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2324 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1681. Description: axIs1681 [pie-1p::GFP::histone H2B::him-3 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2329 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1488. Description: axIs1488 [pCG176/ mCherry::patr-1]. Reference: Gallo CM, et al. Dev Biol. 2008 Nov 1;323(1):76-87. Mutagen: Outcrossed: x Made by: Christopher Gallo Received: 11/19/10 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2330 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1488; axIs????. Description: axIs1488 [pie-1p::mCherry::patr-1::pie-1 3'UTR + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. axIs??? [nmy-2p::pgl-1::GFP::patr-1::nmy-2 3'UTR]. Reference: Gallo CM, et al. Science. 2010 Dec 17;330(6011):1685-9. Mutagen: Outcrossed: x Made by: Christopher Gallo Received: 05/09/11 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2333 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1688. Description: axIs1688 [pie-1p::GFP::histone H2B::mex-3 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2336 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1691. Description: axIs1691 [pie-1p::GFP::H2B::him-3 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Mutagen: Outcrossed: x Made by: C. Merritt Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2342 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1630. Description: axIs1630 [daz-1p::GFP::histone H2B::tbb-2 3'utr + unc-119(+)]. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2347 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1694. Description: axIs1694 [gld-1p::GFP::histone H2B::tbb-2 3'utr + unc-119(+)]. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2349 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1696. Description: axIs1696 [pie-1p::GFP::histone H2B:pgl-3 3'utr , unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2365 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1702. Description: axIs1702 [pie-1p::GFP::fbf-2 ORF::fbf-2 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2366 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1703. Description: axIs1703 [spe-11p::GFP::histone H2B::tbb-2 3'utr + unc-119(+)]. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2373 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1709. Description: axIs1709 [pie-1p::GFP::histone H2B:lip-1M1M2 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Transgene is slightly unstable. Pick non-Unc, GFP+ worms to maintain. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2377 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1713. Description: axIs1713 [pie-1p::GFP::histone H2B::mes-3 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Transgene is very unstable. Pick non-Unc, GFP+ worms to maintain. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2379 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1715. Description: axIs1715 [pie-1p::GFP::histone H2B::pie-1 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2380 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1716. Description: axIs1716 [pie-1p::GFP::histone H2B::unc-54 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Transgene is slightly unstable. Pick non-Unc, GFP+ worms to maintain. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2381 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1717. Description: axIs1717 [pie-1p::GFP::histone H2B::spe-41 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2416 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1742. Description: axIs1742 [lip-1p::GFP::histone H2B::tbb-2 3'utr + unc-119(+)]. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2418 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1721. Description: axIs1721 [pie-1p::GFP::histone H2B::puf-5 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2423 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1722. Description: axIs1722 [pie-1p::GFP::histone H2B::fog-1 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2427 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1751. Description: axIs1751 [pie-1p::GFP::histone H2B::pos-1 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Transgene is slightly unstable. Pick non-Unc, GFP+ worms to maintain. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2428 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1752. Description: axIs1752 [fbf-1p::GFP::histone H2B:tbb-2 3'utr + unc-119(+)]. Maintaining at 25C might reduce the chance of transgene silencing. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2432 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1756. Description: axIs1756 [fbf-2p::GFP::histone H2B:tbb-2 3'utr + unc-119(+)]. Maintaining at 25C might reduce the chance of transgene silencing. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2434 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1758. Description: axIs1758 [spn-4p::GFP::histone H2B:tbb-2 3'utr + unc-119(+)]. Transgene is slightly unstable. Pick non-Unc, GFP+ worms to maintain. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2435 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1759. Description: axIs1759 [msp-56p::GFP::histone H2B:tbb-2 3'utr + unc-119(+)]. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2436 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1723. Description: axIs1723 [pie-1p::GFP::histone H2B:gld-1 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2452 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1490. Description: axIs1490 [pCG230; pie-1p::GFP::ccf-1::ccf-1 3'UTR + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Reference: Dev Bio (2008) 323(1):76-87. Mutagen: Bombardment Outcrossed: x Made by: Christopher Gallo Received: 03/09/10 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2458 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1735. Description: axIs1735 [pie-1p::LAP tag::npp-10 (full length) + unc-119(+)]. Maintain at 25C to maintain transgene expression. Mutagen: Outcrossed: x Made by: E Voronina Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2464 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1779. Description: axIs1779 [pie-1p::mCherry::H2B::tbb-2 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Mutagen: Outcrossed: x Made by: E Voronina Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2471 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1775. Description: axIs1775 [pie-1p::GFP::histone H2B:gld-1 M1M2 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Pick wild-type worms to maintain. Mutagen: Bombardment Outcrossed: x0 Made by: Chris Meritt Received: 09/26/08 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2513 Species: Caenorhabditis elegans Genotype: fbf-1(ok91) II; axIs1723. Description: axIs1723 [pie-1p::GFP::H2B::gld-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Unknown if ed3 is still in background. Mutagen: Outcrossed: x Made by: C. Merritt Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2523 Species: Caenorhabditis elegans Genotype: fbf-2(q738) II; axIs1722. Description: axIs1722 [pie-1p::GFP::H2B::fog-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Unknown if ed3 is still in background. Mutagen: Outcrossed: x Made by: C. Merritt Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2525 Species: Caenorhabditis elegans Genotype: fbf-1(ok91) II; axIs1722. Description: axIs1722 [pie-1p::GFP::H2B::fog-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Unknown if ed3 is still in background. Mutagen: Outcrossed: x Made by: C. Merritt Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2549 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1957. Description: axIs1957 [pie-1p::Dendra2::TEV::S-peptide::mex-5::pie-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Mutagen: Outcrossed: x Made by: EGG Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2550 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1962. Description: axIs1962 [pie-1p::Dendra2::TEV::S-peptide::mex-5(S458A)::pie-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Mutagen: Outcrossed: x Made by: EGG Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2553 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1958. Description: axIs1958 [pie-1p::Dendra2::TEV::S-peptide::mex-5(aa345-aa468)::pie-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Mutagen: Outcrossed: x Made by: EGG Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2560 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1959. Description: axIs1959 [pie-1p::Dendra2::TEV::S-peptide::pie-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Mutagen: Outcrossed: x Made by: EGG Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2593 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1961. Description: axIs1961 [pie-1p::Dendra2::TEV::S-peptide::mex-5(S404A)::pie-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Mutagen: Outcrossed: x Made by: EEG Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2607 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1963. Description: axIs1963 [pie-1p::Dendra2::TEV::S-peptide::mex-5(S404A S458A)::pie-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Mutagen: Outcrossed: x Made by: EEG Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2621 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1849. Description: axIs1849 [pie-1p::GFP::H2B::htp-3 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. GFP expression in adult germline; somatic and germline expression in embryos. Mutagen: Outcrossed: x Made by: Chris Meritt Received: 04/16/13 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2623 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1851. Description: axIs1851 [pie-1p::GFP::H2B::rad-51 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. GFP expression in adult germline; somatic and germline expression in embryos. Mutagen: Outcrossed: x Made by: Chris Meritt Received: 04/16/13 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2636 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1960. Description: axIs1960 [pie-1p::Dendra2::TEV::S-peptide::mex-5(C286S C292S C331S C337S)::pie-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Mutagen: Outcrossed: x Made by: EEG Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2647 Species: Caenorhabditis elegans Genotype: axIs1928; axIs1182. Description: axIs1928 [mCherry::par-6]. axIs1182 [GFP::par-2]. Maintain at 25C to retain transgene expression. [NOTE: axIs1182 [GFP::par-2] might not be integrated; the CGC has received reports that it does not segregate to all progeny.] Mutagen: Outcrossed: x Made by: Seth Zonies Received: 01/18/13 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2648 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1928. Description: axIs1928 [mCherry::par-6]. Mutagen: Outcrossed: x Made by: Seth Zonies Received: 11/19/10 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2652 Species: Caenorhabditis elegans Genotype: ect-2(ax751) II; zuIs45 V. Description: zuIs45 [nmy-2::NMY-2::GFP + unc-119(+)] V. Temperature-sensitive Maternal-effect lethal at 25 C. Maintain at 15-20 C. Mutagen: Outcrossed: x Made by: Seth Zonies Received: 11/19/10 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2657 Species: Caenorhabditis elegans Genotype: ect-2(ax751) II; axIs1928; axIs1182. Description: axIs1928 [mCherry::par-6]. axIs1182 [GFP::par-2]. Temperature-sensitive Maternal-effect lethal at 25 C. Maintain at 15-20 C. Mutagen: Outcrossed: x Made by: Seth Zonies Received: 11/19/10 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2671 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1864. Description: axIs1864 [pie-1p::GFP::H2B::zim-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. GFP expression in mitotic germline; variable, sometimes weak, GFP expression in pachytene stage. Mutagen: Outcrossed: x Made by: Chris Meritt Received: 04/16/13 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2686 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1844. Description: axIs1844 [GFP::npp-7 + unc-119(+)]. Maintain at 25C to maintain transgene expression. Mutagen: Outcrossed: x Made by: E Voronina Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2688 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1927. Description: axIs1927 [LAPtag::glh-1 + unc-119(+)]. Maintain at 25C to maintain transgene expression. Mutagen: Outcrossed: x Made by: E Voronina Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2691 Species: Caenorhabditis elegans Genotype: npp-10(ok467)/qC1 [dpy-19(e1259) glp-1(q339) qIs26] III. Description: qIs26 [lag-2::GFP + rol-6(su1006)]. ok467 deletion balanced by glp-1- and dpy-19-marked recombination suppressor. qIs26 was integrated into qC1 and in the process made qC1 homozygous lethal. Heterozygotes are Rollers and GFP+ in the distal tip cell, and segregate WT Rol, lethal qC1 homozygotes, and npp-10 homozygotes (Emb or early Larval lethal). Pick WT GFP+ Rol and check for correct segregation of progeny to maintain. Mutagen: Outcrossed: x Made by: G. Sedoux Received: 05/17/11 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2704 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1890. Description: axIs1890 [pie-1p::GFP::H2B::msh-5 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Weak GFP expression in mitotic germline; GFP expression in pachytene, oocytes, and embryos. Mutagen: Outcrossed: x Made by: Chris Meritt Received: 04/16/13 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2730 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1895. Description: axIs1895 [pie-1p::GFP::H2B::rec-8 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. GFP expression in adult germline; somatic and germline expression in embryos. Mutagen: Outcrossed: x Made by: Chris Meritt Received: 04/16/13 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2749 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1914. Description: axIs1914 [syp-3p::GFP::syp-3 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. GFP expression in meiotic germline. Mutagen: Outcrossed: x Made by: Chris Meritt Received: 04/16/13 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2754 Species: Caenorhabditis elegans Genotype: ect-2(ax751) II. Description: Temperature-sensitive Maternal-effect lethal at 25 C. Maintain at 15-20 C. Mutagen: Outcrossed: x3 Made by: Seth Zonies Received: 11/19/10 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2756 Species: Caenorhabditis elegans Genotype: ect-2(ax751) II; par-3(it71) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III; zuIs45 V. Description: zuIs45 [nmy-2::NMY-2::GFP + unc-119(+)] V. Temperature-sensitive. Maternal-effect lethal at 25 C. Maintain at 15-20 C. Segregates Unc Mel, non-Unc Mel, non-Unc Ste. Reference: Zonies S, et al. Development. 2010 May;137(10):1669-77. Mutagen: Outcrossed: x Made by: Seth Zonies Received: 11/19/10 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2759 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1929. Description: axIs1929 [nmy-2::GFP + mCherry::par-2]. Reference: Zonies S, et al. Development. 2010 May;137(10):1669-77. Mutagen: Outcrossed: x Made by: Seth Zonies Received: 10/10/11 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2773 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1946. Description: axIs1946 [pie-1p::Dendra::pgl-1::pgl-1 3'UTR + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Reference: Gallo CM, et al. Science. 2010 Dec 17;330(6011):1685-9. Mutagen: Outcrossed: x Made by: Christopher Gallo Received: 05/09/11 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2787 Species: Caenorhabditis elegans Genotype: pptr-1(tm3103) V. Description: pptr-1(tm3103) is a temperature-sensitive allele. Maintain at 15C. Fully penetrant P granule partitioning defect at 20-24C. Low penetrance of sterilty observed at 24-26C. Reference: Gallo CM, et al. Science. 2010 Dec 17;330(6011):1685-9. Mutagen: TMP/UV Outcrossed: x6 Made by: S. Mitani / C. Gallo Received: 04/13/11 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2791 Species: Caenorhabditis elegans Genotype: pptr-1(tm3103) V; axIs1448. Description: axIs1448 [pie-1p::GFP::H2B::nos-2(wt) 3'UTR + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C.Reference: Gallo CM, et al. Science. 2010 Dec 17;330(6011):1685-9. Mutagen: Outcrossed: x Made by: Christopher Gallo Received: 05/09/11 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2794 Species: Caenorhabditis elegans Genotype: pptr-1(tm3103) V; axIs1462. Description: axIs1462 [pie-1p::GFP::pie-1::pie-1 3'UTR + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C.Reference: Gallo CM, et al. Science. 2010 Dec 17;330(6011):1685-9. Mutagen: Outcrossed: x Made by: Christopher Gallo Received: 05/09/11 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2800 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1964. Description: axIs1964 [mex-5p::GFP::TEV::FLAG::mex-5::mex-5 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Mutagen: Outcrossed: x Made by: EG Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2801 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1965. Description: axIs1965 [mex-5p::GFP::TEV::FLAG::mex-5::mex-5 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Mutagen: Outcrossed: x Made by: EG Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2802 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1950. Description: axIs1950 [mex-5p::Dendra2::TEV::S-peptide::mex-5RR::mex-5 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Mutagen: Outcrossed: x Made by: EG Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2804 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1951. Description: axIs1951 [pie-1p::Dendra2::TEV::S-peptide::mex-5RR(S404A)::mex-5 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Mutagen: Outcrossed: x Made by: EG Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2814 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1933. Description: axIs1933 [(pFM034) GFP::par-2 RNAi-resistant + unc-119(+)]. Mutagen: Outcrossed: x Made by: G. Seydoux Received: 03/20/13 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2815 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1934. Description: axIs1934 [(pFM035) GFP::par-2 RNAi-resistant [R183-5A] + unc-119(+)]. Mutagen: Outcrossed: x Made by: G. Seydoux Received: 03/20/13 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2816 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1935. Description: axIs1935 [(pFM036) GFP::par-2 RNAi-resistant [K162A] + unc-119(+)]. Mutagen: Outcrossed: x Made by: G. Seydoux Received: 03/20/13 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2817 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1936. Description: axIs1936 [(pFM037) GFP::par-2 RNAi-resistant [R163A] + unc-119(+)]. Mutagen: Outcrossed: x Made by: G. Seydoux Received: 03/20/13 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2825 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1943. Description: axIs1943 [(pFM050) mCherry::mlc-4 + unc-119(+)]. Mutagen: Outcrossed: x Made by: G. Seydoux Received: 03/20/13 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2835 Species: Caenorhabditis elegans Genotype: pptr-1(tm3103) V; axIs1504. Description: axIs1504 [pie-1p::LAP::mex-5::pie-1 3'UTR + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Reference: Gallo CM, et al. Science. 2010 Dec 17;330(6011):1685-9. Mutagen: Outcrossed: x Made by: Christopher Gallo Received: 03/15/25 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2836 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs????. Description: axIs??? [nmy-2p::pgl-1::GFP::patr-1::nmy-2 3'UTR]. Reference: Gallo CM, et al. Science. 2010 Dec 17;330(6011):1685-9. Mutagen: Outcrossed: x Made by: Christopher Gallo Received: 05/09/11 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2839 Species: Caenorhabditis elegans Genotype: pptr-1(tm3103) V; axIs1522; axIs1929. Description: axIs1522 [pie-1p::GFP::pgl-1::pgl-1 3'UTR + unc-119(+)]. axIs1929 [pie-1p::mCherry::par-2::pie-1 3'UTR + unc-119(+)]. Transgenes are prone to silencing -- maintain at 25C.Reference: Gallo CM, et al. Science. 2010 Dec 17;330(6011):1685-9. Mutagen: Outcrossed: x Made by: Christopher Gallo Received: 05/09/11 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2840 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs???? ; axIs1731. Description: axIs??? [nmy-2p::pgl-1::GFP::patr-1::nmy-2 3'UTR]. axIs1731 [pie-1p::mCherry::mex-5::pie-1 3'UTR + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. Reference: Gallo CM, et al. Science. 2010 Dec 17;330(6011):1685-9. Mutagen: Outcrossed: x Made by: Christopher Gallo Received: 05/09/11 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2841 Species: Caenorhabditis elegans Genotype: pptr-1(tm3103) V; axIs1522. Description: axIs1522 [pie-1p::GFP::pgl-1::pgl-1 3'UTR + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C.Reference: Gallo CM, et al. Science. 2010 Dec 17;330(6011):1685-9. Mutagen: Outcrossed: x Made by: Christopher Gallo Received: 05/09/11 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2874 Species: Caenorhabditis elegans Genotype: pgl-1(ct131) him-3(e1147) III; ozIs5. Description: ozIs5 [gld-1::GFP + unc-119(+)]. Mutagen: Outcrossed: x Made by: E Voronina Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2877 Species: Caenorhabditis elegans Genotype: fbf-2(q738) II; pgl-1(ct131) him-3(e1147) III; axIs1722. Description: axIs1722 [pie-1p::GFP::H2B::fog-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Unknown if ed3 is still in background. Mutagen: Outcrossed: x Made by: E Voronina Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2883 Species: Caenorhabditis elegans Genotype: fbf-1(ok91) II; pgl-1(ct131) him-3(e1147) III; axIs1722. Description: axIs1722 [pie-1p::GFP::H2B::fog-1 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Unknown if ed3 is still in background. Mutagen: Outcrossed: x Made by: E Voronina Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2889 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1955. Description: axIs1955 [mex-5p::Dendra2::TEV::S-peptide::mex-5RR(S458A)::mex-5 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Mutagen: Outcrossed: x Made by: EG Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2890 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs1956. Description: axIs1956 [mex-5p::Dendra2::TEV::S-peptide::mex-5RR(R274E K318E)::mex-5 3'UTR + unc-119(+)]. Maintain at 25C to maintain transgene expression. Mutagen: Outcrossed: x Made by: EG Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2919 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs2000. Description: axIs2000 [LAPtag::fbf-2::fbf-2 3'UTR]. Maintain at 25C to maintain transgene expression. Mutagen: Outcrossed: x Made by: E Voronina Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2929 Species: Caenorhabditis elegans Genotype: fbf-1(ok91) II; axIs2000. Description: axIs2000 [LAPtag::fbf-2::fbf-2 3'UTR]. Maintain at 25C to maintain transgene expression. Mutagen: Outcrossed: x Made by: E Voronina Received: 09/11/12 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2932 Species: Caenorhabditis elegans Genotype: unc-24(e1172) mbk-2(pk1427) IV/nT1[let-?(m435)] (IV;V); ddEx16. Description: ddEx16 [pgl-1::TY1::EGFP::3xFLAG(92C12) + Cbr-unc-119(+)]. Maintain at 25C to retain transgene expression. Heterozygotes are Unc and segregate Uncs, dead eggs and WT. Reference: Wang JT, et al. eLife 2014;3:e04591. Mutagen: Outcrossed: x0 Made by: Jennifer Wang Received: 02/20/15 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2996 Species: Caenorhabditis elegans Genotype: cdc-37(ax2001) II; mbk-2(dd5) IV. Description: Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41. Mutagen: EMS Outcrossed: x4 Made by: Seydoux Lab Received: 07/17/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2997 Species: Caenorhabditis elegans Genotype: plk-1(ax2002) III; mbk-2(dd5) IV. Description: Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41. Mutagen: EMS Outcrossed: x4 Made by: Seydoux Lab Received: 07/17/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2998 Species: Caenorhabditis elegans Genotype: emb-30(ax2003) III; mbk-2(dd5) IV. Description: Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41. Mutagen: EMS Outcrossed: x4 Made by: Seydoux Lab Received: 07/17/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH2999 Species: Caenorhabditis elegans Genotype: mbk-2(dd5ax2004) IV. Description: Maintain at 25C. Intragenic suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41. Mutagen: EMS Outcrossed: x4 Made by: Seydoux Lab Received: 07/17/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3000 Species: Caenorhabditis elegans Genotype: mbk-2(dd5ax2005) IV. Description: Maintain at 25C. Intragenic suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41. Mutagen: EMS Outcrossed: x4 Made by: Seydoux Lab Received: 07/17/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3002 Species: Caenorhabditis elegans Genotype: mbk-2(dd5ax2007) IV. Description: Maintain at 25C. Intragenic suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41. Mutagen: EMS Outcrossed: x4 Made by: Seydoux Lab Received: 07/17/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3003 Species: Caenorhabditis elegans Genotype: plk-1(ax2008) III; mbk-2(dd5) IV. Description: Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41. Mutagen: EMS Outcrossed: x4 Made by: Seydoux Lab Received: 07/17/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3004 Species: Caenorhabditis elegans Genotype: tat-4(ax2009) II; mbk-2(dd5) IV. Description: Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41. Mutagen: EMS Outcrossed: x4 Made by: Seydoux Lab Received: 07/17/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3005 Species: Caenorhabditis elegans Genotype: such-1(ax2010) III; mbk-2(dd5) IV. Description: Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41. Mutagen: EMS Outcrossed: x4 Made by: Seydoux Lab Received: 07/17/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3006 Species: Caenorhabditis elegans Genotype: mus-101(ax2011) I; mbk-2(dd5) IV. Description: Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41. Mutagen: EMS Outcrossed: x4 Made by: Seydoux Lab Received: 07/17/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3009 Species: Caenorhabditis elegans Genotype: fzy-1(ax2014) II; mbk-2(dd5) IV. Description: Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41. Mutagen: EMS Outcrossed: x4 Made by: Seydoux Lab Received: 07/17/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3011 Species: Caenorhabditis elegans Genotype: cdc-37(ax2001) II; unc-119(ed3) orIs1 III; mbk-2(dd5) IV. Description: orIs1 [pie-1p::GFP::mei-1 + unc-119(+)] III. Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41. Mutagen: Outcrossed: x Made by: Seydoux Lab Received: 07/17/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3012 Species: Caenorhabditis elegans Genotype: plk-1(ax2002) unc-119(ed3) orIs1 III; mbk-2(dd5) IV. Description: orIs1 [pie-1p::GFP::mei-1 + unc-119(+)] III. Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41. Mutagen: Outcrossed: x Made by: Seydoux Lab Received: 07/17/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3013 Species: Caenorhabditis elegans Genotype: unc-119(ed3) orIs1 III; mbk-2(dd5ax2004) IV. Description: orIs1 [pie-1p::GFP::mei-1 + unc-119(+)] III. Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41. Mutagen: Outcrossed: x Made by: Seydoux Lab Received: 07/17/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3016 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs2076. Description: axIs2076 [meg-3p::GFP::meg-3::meg-3 3'UTR + unc-119(+)]. Maintain at 25C and pick non-Unc to retain transgene expression. Reference: Wang JT, et al. eLife 2014;3:e04591. Mutagen: Outcrossed: x0 Made by: Jennifer Wang Received: 02/20/15 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3019 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; axIs2076; axIs2077. Description: axIs2076 [meg-3p::GFP::meg-3::meg-3 3'UTR + unc-119(+)]. axIs2077 [pie-1p::GFP::pgl-3::pie-1 3'UTR]. Maintain at 25C and pick non-Unc to retain transgene expression. Reference: Wang JT, et al. eLife 2014;3:e04591. Mutagen: Outcrossed: x0 Made by: Jennifer Wang Received: 02/20/15 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3055 Species: Caenorhabditis elegans Genotype: meg-3(tm4259) X. Description: Mild embryonic P granule defect. Reference: Wang JT, et al. eLife 2014;3:e04591. Mutagen: TMP/UV Outcrossed: x6 Made by: S. Mitani & J. Wang Received: 02/20/15 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3064 Species: Caenorhabditis elegans Genotype: pptr-1(tm3103) V; axIs2076. Description: axIs2076 [meg-3p::GFP::meg-3::meg-3 3'UTR + unc-119(+)]. Maintain at 25C and pick non-Unc to retain transgene expression. Reference: Wang JT, et al. eLife 2014;3:e04591. Mutagen: Outcrossed: x0 Made by: Jennifer Wang Received: 02/20/15 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3075 Species: Caenorhabditis elegans Genotype: tat-4(ax2009) II; unc-119(ed3) orIs1 III; mbk-2(dd5) IV. Description: orIs1 [pie-1p::GFP::mei-1 + unc-119(+)] III. Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41. Mutagen: Outcrossed: x Made by: Seydoux Lab Received: 07/17/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3076 Species: Caenorhabditis elegans Genotype: unc-119(ed3) such-1(ax2010) orIs1 III; mbk-2(dd5) IV. Description: orIs1 [pie-1p::GFP::mei-1 + unc-119(+)] III. Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41. Mutagen: Outcrossed: x Made by: Seydoux Lab Received: 07/17/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3078 Species: Caenorhabditis elegans Genotype: apc-1(ax2012) II; unc-119(ed3) orIs1 III; mbk-2(dd5) IV. Description: orIs1 [pie-1p::GFP::mei-1 + unc-119(+)] III. Maintain at 25C. Suppressor of dd5. Formerly known as mat-2. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41. Mutagen: Outcrossed: x Made by: Seydoux Lab Received: 07/17/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3080 Species: Caenorhabditis elegans Genotype: fzy-1(ax2014) II; unc-119(ed3) orIs1 III; mbk-2(dd5) IV. Description: orIs1 [pie-1p::GFP::mei-1 + unc-119(+)] III. Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41. Mutagen: Outcrossed: x Made by: Seydoux Lab Received: 07/17/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3086 Species: Caenorhabditis elegans Genotype: emb-30(ax2003) unc-119(ed3) orIs1 III; mbk-2(dd5) IV. Description: orIs1 [pie-1p::GFP::mei-1 + unc-119(+)] III. Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41. Mutagen: Outcrossed: x Made by: Seydoux Lab Received: 07/17/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3087 Species: Caenorhabditis elegans Genotype: xpo-2(ax2013) I; unc-119(ed3) orIs1 III; mbk-2(dd5) IV. Description: orIs1 [pie-1p::GFP::mei-1 + unc-119(+)] III. Maintain at 25C. Suppressor of dd5. Reference: Wang Y, et al. G3 (Bethesda). 2014 Feb 19;4(2):231-41. Mutagen: Outcrossed: x Made by: Seydoux Lab Received: 07/17/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3134 Species: Caenorhabditis elegans Genotype: swan-1(ax2045[V5::swan-1]) V. Description: V5 tag inserted at N-terminus of swan-1. Reference: Paix A, et al. Genetics. 2014 Sep 23. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Deepika Calidas Received: 11/11/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3136 Species: Caenorhabditis elegans Genotype: swan-1(ax2047[Myc::swan-1]) V. Description: Myc tag inserted at N-terminus of swan-1. Reference: Paix A, et al. Genetics. 2014 Sep 23. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Deepika Calidas Received: 11/11/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3147 Species: Caenorhabditis elegans Genotype: ltIs37 IV; meg-3(tm4259) X; axIs1522. Description: axIs1522 [pie-1p::GFP::pgl-1::pgl-1 3'UTR + unc-119(+)]. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Maintain at 25C and pick non-Unc to retain transgene expression. Reference: Wang JT, et al. eLife 2014;3:e04591. Mutagen: Outcrossed: x0 Made by: Jennifer Wang Received: 02/20/15 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3148 Species: Caenorhabditis elegans Genotype: ltIs37 IV; meg-1(vr10) X; axIs1522. Description: axIs1522 [pie-1p::GFP::pgl-1::pgl-1 3'UTR + unc-119(+)]. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Reference: Wang JT, et al. eLife 2014;3:e04591. Mutagen: Outcrossed: x0 Made by: Jennifer Wang Received: 02/20/15 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3149 Species: Caenorhabditis elegans Genotype: ltIs37 IV; meg-3(tm4259) meg-4(ax2026) X; axIs1522. Description: axIs1522 [pie-1p::GFP::pgl-1::pgl-1 3'UTR + unc-119(+)]. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Maintain at 25C and pick non-Unc to retain transgene expression. Reference: Wang JT, et al. eLife 2014;3:e04591. Mutagen: Outcrossed: x0 Made by: Jennifer Wang Received: 02/20/15 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3150 Species: Caenorhabditis elegans Genotype: ltIs37 IV; meg-1(vr10) meg-3(tm4259) X; axIs1522. Description: axIs1522 [pie-1p::GFP::pgl-1::pgl-1 3'UTR + unc-119(+)]. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Maintain at 25C and pick non-Unc to retain transgene expression. Reference: Wang JT, et al. eLife 2014;3:e04591. Mutagen: Outcrossed: x0 Made by: Jennifer Wang Received: 02/20/15 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3153 Species: Caenorhabditis elegans Genotype: meg-3(tm4259) X; axIs2076. Description: axIs2076 [meg-3p::GFP::meg-3::meg-3 3'UTR + unc-119(+)]. Maintain at 25C and pick non-Unc to retain transgene expression. Reference: Wang JT, et al. eLife 2014;3:e04591. Mutagen: Outcrossed: x0 Made by: Jennifer Wang Received: 02/20/15 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3155 Species: Caenorhabditis elegans Genotype: ltIs37 IV; pptr-1(tm3103) V; meg-3(tm4259) X; axIs1522. Description: axIs1522 [pie-1p::GFP::pgl-1::pgl-1 3'UTR + unc-119(+)]. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Maintain at 25C and pick non-Unc to retain transgene expression. Reference: Wang JT, et al. eLife 2014;3:e04591. Mutagen: Outcrossed: x0 Made by: Jennifer Wang Received: 02/20/15 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3156 Species: Caenorhabditis elegans Genotype: ltIs37 IV; pptr-1(tm3103) V; meg-1(vr10) X; axIs1522. Description: axIs1522 [pie-1p::GFP::pgl-1::pgl-1 3'UTR + unc-119(+)]. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. Maintain at 25C and pick non-Unc to retain transgene expression. Reference: Wang JT, et al. eLife 2014;3:e04591. Mutagen: Outcrossed: x0 Made by: Jennifer Wang Received: 02/20/15 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3158 Species: Caenorhabditis elegans Genotype: swan-1&swan-2(ax2071) V. Description: Deletion of the operon CEOP 5400, removing both swan-1 and swan-2 (V: 13801593-13807217) and insertion of ATTTGTTCAGACAATAAGCTNGAAATC. No reported phenotype. Reference: Paix A, et al. Genetics. 2014 Sep 23. Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Deepika Calidas Received: 11/11/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3159 Species: Caenorhabditis elegans Genotype: swan-1&swan-2(ax2072) V. Description: Deletion of the operon CEOP 5400, removing both swan-1 and swan-2 (V: 13801629-13807223). No reported phenotype. Reference: Paix A, et al. Genetics. 2014 Sep 23. Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Deepika Calidas Received: 11/11/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3176 Species: Caenorhabditis elegans Genotype: gtbp-1(ax2029) IV. Description: Deletion/insertion (AGCTAGC) of a STOP codon/frameshift near the ATG between IV: 10128909...10128934. Reference: Paix A, et al. Genetics. 2014 Sep 23. Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Alexandre Paix Received: 11/11/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3180 Species: Caenorhabditis elegans Genotype: nos-2(ax2033) II. Description: Maintain at 20C. ax2033 was produced by replacing +16 to +42 with TCGACTCTCGAACGATCGTAATAG in the first exon of nos-2. ax2033 mutants are sterile when fed nos-1 dsRNA. Reference: Paix A, et al. Genetics. 2014 Sep 23. Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Chih-Yung Lee Received: 11/11/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3182 Species: Caenorhabditis elegans Genotype: gtbp-1(ax2035[gtbp-1::TetraCys]) IV. Description: Maintain at 20-25C. ax2035 was produced by mutation of the sgRNA site and insertion of TetraCys tag at the C-terminus of gtbp-1. Substitution/insertion of the sequence GCAGCATCCTGGGCAGCAATTTTGTCCGGCATTTTGGAAACCGCTGCGCATTCCTCCAC GT between IV: 10127239...10127283. Reference: Paix A, et al. Genetics. 2014 Sep 23. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Alexandre Paix Received: 11/11/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3184 Species: Caenorhabditis elegans Genotype: gtbp-1(ax2037([gtbp-1::Myc]) IV. Description: Maintain at 20-25C. ax2037 was produced by mutation of the sgRNA site and insertion of Myc tag at the C-terminus of gtbp-1. Substitution/insertion of the sequence CAGATCCTCTTCTGATATCAGTTTTTGTTCATTTTGTCCCGCATTTTGGAAACCGCTAC GCATTCCTCCACGC between IV: 10127239...10127283. Reference: Paix A, et al. Genetics. 2014 Sep 23. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Alexandre Paix Received: 11/11/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3186 Species: Caenorhabditis elegans Genotype: gtbp-1(ax2039[gtbp-1::3xFlag]) IV. Description: Maintain at 20-25C. ax2039 was produced by insertion of 3xFLAG tag at the C-terminus of gtbp-1 by NHEJ. Substitution/insertion of the sequence CTTGTCATCGTCATCCTTGTAATCGATATCATGATCTTTATAATCACCGTCATGGTCTT TGTAGTCCTCCACGAGGAATGCGTGAGGAAATCGTGGA between IV: 10127239...10127269. Reference: Paix A, et al. Genetics. 2014 Sep 23. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Alexandre Paix Received: 11/11/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3188 Species: Caenorhabditis elegans Genotype: mex-5(ax2041[3xFLAG::mex-5]) IV. Description: Maintain at 20C. 3xFLAG-tagged MEX-5. Reference: Paix A, et al. Genetics. 2014 Sep 23. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jarrett Smith Received: 11/11/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3190 Species: Caenorhabditis elegans Genotype: mex-5(ax2043[OLLAS::mex-5]) IV. Description: Maintain at 20C. OLLAS-tagged MEX-5. Reference: Paix A, et al. Genetics. 2014 Sep 23. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jarrett Smith Received: 11/11/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3193 Species: Caenorhabditis elegans Genotype: nos-2(ax2049[3XFLAG::nos-2]) II. Description: Maintain at 20C. FLAG::NOS-2 can be detected from P4 to Z2/Z3 stage. Reference: Paix A, et al. Genetics. 2014 Sep 23. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Chih-Yung Lee Received: 11/11/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3195 Species: Caenorhabditis elegans Genotype: mbk-2(ax2051[V5::mbk-2]) IV. Description: V5 tag inserted at the N-terminus of mbk-2 isoform a. Reference: Paix A, et al. Genetics. 2014 Sep 23. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Yuemeng Wang Received: 11/11/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3197 Species: Caenorhabditis elegans Genotype: gtbp-1(ax2053[gtbp-1::GFP]) IV. Description: Maintain at 20-25C. Insertion of GFP cDNA (from pCM1.53, no ATG/no STOP) at the C-terminus of gtbp-1 between IV: 10127266...10127267. Reference: Paix A, et al. Genetics. 2014 Sep 23. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Alexandre Paix Received: 11/11/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3199 Species: Caenorhabditis elegans Genotype: gtbp-1(ax2055[gtbp-1::GFP]) IV. Description: Maintain at 20-25C. Insertion of GFP cDNA (from pCM1.53, no ATG/no STOP) at the C-terminus of gtbp-1: ATTTTGTCCCGCATTTTGGAAACCGCTACGCATTCCTCCACGC(GFP) between IV: 10127239-10127283. sgRNA site was mutated to avoid Cas9 re-cutting. Reference: Paix A, et al. Genetics. 2014 Sep 23. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Alexandre Paix Received: 11/11/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3201 Species: Caenorhabditis elegans Genotype: fbf-2(ax2057[fbf-2::GFP]) II. Description: Maintain at 20-25C. ax2057 was produced by mutation of the sgRNA site and insertion of GFP cDNA at the C-terminus of fbf-2. Insertion of GFP cDNA (from pCM1.53, no ATG/no STOP) at the C-terminus of fbf-2: ATCATCGCCGTGACTACCA(GFP) between II:6089145...6089165. sgRNA site was mutated to avoid Cas9 re-cutting. Reference: Paix A, et al. Genetics. 2014 Sep 23. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Alexandre Paix Received: 11/11/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3203 Species: Caenorhabditis elegans Genotype: mes-2(ax2059[mes-2::GFP]) II. Description: Maintain at 20C. Insertion of GFP cDNA (from pCM1.53, no ATG/no STOP) at the C-terminus of mes-2 between II:14388297...14388298. Reference: Paix A, et al. Genetics. 2014 Sep 23. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Alexandre Paix Received: 11/11/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3205 Species: Caenorhabditis elegans Genotype: lin-15B(ax2061[lin-15B::GFP]) X. Description: Maintain at 20C. Nuclear expression of lin-15B::GFP in oocytes and embryos. Reference: Paix A, et al. Genetics. 2014 Sep 23. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Yuemeng Wang Received: 11/11/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3207 Species: Caenorhabditis elegans Genotype: deps-1(ax2063[deps-1::GFP]) I. Description: Maintain at 20C. GFP inserted at N-terminus of deps-1. Reference: Paix A, et al. Genetics. 2014 Sep 23. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: J Smith & T Lu Received: 11/11/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3209 Species: Caenorhabditis elegans Genotype: mex-6(ax2065[mex-6::GFP]) II. Description: GFP-tagged mex-6. Reference: Paix A, et al. Genetics. 2014 Sep 23. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Paix/J Smith/Y Wang Received: 03/23/15 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3212 Species: Caenorhabditis elegans Genotype: gtbp-1(ax2068) IV. Description: 1.6kb deletion in gtbp-1 between IV: 10127256...10128923. Homozygous viable. Reference: Paix A, et al. Genetics. 2014 Sep 23. Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Alexandre Paix Received: 03/15/25 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3215 Species: Caenorhabditis elegans Genotype: gtbp-1(ax2073) IV. Description: 1.6kb deletion in gtbp-1 between IV: 10127264...10128913 and insertion of NheI restriction site (GCTAGC). Homozygous viable. Reference: Paix A, et al. Genetics. 2014 Sep 23. Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Alexandre Paix Received: 11/11/14 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3225 Species: Caenorhabditis elegans Genotype: meg-3(tm4259) meg-4(ax2026) X. Description: P granule defect. High sterility. Reference: Wang JT, et al. eLife 2014;3:e04591. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jennifer Wang Received: 02/20/15 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3229 Species: Caenorhabditis elegans Genotype: meg-1(vr10) meg-3(tm4259) X. Description: P granule defect. Some sterility. Reference: Wang JT, et al. eLife 2014;3:e04591. Mutagen: Outcrossed: x0 Made by: Jennifer Wang Received: 02/20/15 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3230 Species: Caenorhabditis elegans Genotype: pgl-3(bn104) V; axIs2076. Description: axIs2076 [meg-3p::GFP::meg-3::meg-3 3'UTR + unc-119(+)]. Maintain at 25C and pick non-Unc to retain transgene expression. Reference: Wang JT, et al. eLife 2014;3:e04591. Mutagen: Outcrossed: x0 Made by: Jennifer Wang Received: 02/20/15 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3247 Species: Caenorhabditis elegans Genotype: meg-4(ax2080[meg-4::FLAG]) X. Description: C-terminal FLAG insertion in endogenous meg-4 locus. Reference: Wang JT, et al. eLife 2014;3:e04591. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jennifer Wang Received: 02/20/15 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3248 Species: Caenorhabditis elegans Genotype: meg-4(ax2081) X. Description: Deletion removing 733 base pairs upstream of start and the first 2565 bases of the endogenous meg-4 locus. Reference: Wang JT, et al. eLife 2014;3:e04591. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Jennifer Wang Received: 02/20/15 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3269 Species: Caenorhabditis elegans Genotype: pgl-1(ax3122[pgl-1::gfp]) IV. Description: GFP inserted at C terminus of endogenous pgl-1 locus. Reference: Putnam A, et al. Nat Struct Mol Biol. 2019 Mar;26(3):220-226. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Dominique Rasoloson Received: 07/09/19 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3296 Species: C. elegans Genotype: mex-5(ax3050[mCherry::mex-5]) IV. Description: mCherry tag inserted into endogenous mex-5 locus. References: Smith J, et al. eLife. 2016 Dec 3;5:e21337. doi: 10.7554/eLife.21337. PMID: 27914198. Mutagen: Outcrossed: x0 Made by: Andrew Folkmann Received: 04/04/23 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3308 Species: C elegans Genotype: gtbp-1(ax5000[gtbp-1::tagRFP]) IV. Description: tagRFP tag inserted into endogenous gtbp-1 locus. Reference: Lee, CYS, et al. Elife. 020 Jan 24:9:e52896. doi: 10.7554/eLife.52896. PMID: 31975687. Mutagen: Outcrossed: x1 Made by: Sean Lee Received: 02/06/24 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3379 Species: Caenorhabditis elegans Genotype: meg-1(ax4534[meg-1::gfp]) X. Description: GFP tag inserted at C-terminus of the endogenous meg-1 locus by CRISPR. Reference: Cassani M & Seydoux G. Development 2022 Nov 1;149(21):dev200920. doi: 10.1242/dev.200920. PMID: 36196602. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Dominique Rasoloson Received: 11/17/22 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3407 Species: Caenorhabditis elegans Genotype: meg-1(ax4535[meg-1::OLLAS]) X. Description: OLLAS tag inserted at C-terminus of the endogenous meg-1 locus by CRISPR. Reference: Cassani M & Seydoux G. Development 2022 Nov 1;149(21):dev200920. doi: 10.1242/dev.200920. PMID: 36196602. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Helen Schmidt Received: 11/17/22 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3475 Species: Caenorhabditis elegans Genotype: meg-3(ax3055) meg-4(ax3052) X. Description: Embryonic P granules not segregated, 30% maternal effect sterility on average, RNAi insensitivity. meg-3(ax3055) and meg-4(ax3052) are precise deletions of the entire coding sequence made by CRISPR/Cas9, designed to be cut again to make insertions at the endogenous locus. References: Smith, J. et al. Elife. 2016 Dec 3;5:e21337. doi: 10.7554/eLife.21337. PMID: 27914198 Ouyang JPT, et al. Dev Cell. 2019 Sep 23;50(6):716-728.e6. PMID: 31402283 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Helen Schmidt Received: 05/18/21 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3477 Species: Caenorhabditis elegans Genotype: meg-3(ax3051[meg-3::OLLAS]) meg-4(ax3052) X. Description: P granule size reduced, detection of MEG-3 by anti-OLLAS antibody. References: Smith, J. et al. Elife. 2016 Dec 3;5:e21337. doi: 10.7554/eLife.21337. PMID: 27914198 Schmidt H, bioRxiv 2020.10.15.340570 (2020) doi:10.1101/2020.10.15.340570. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Deepika Calidas Received: 05/18/21 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3503 Species: Caenorhabditis elegans Genotype: meg-3(ax3054[meg-3::meGFP]) X. Description: meGFP inserted between P121 and V122 of endogenous MEG-3. Reference: Putnam A, et al. Nat Struct Mol Biol. 2019 Mar;26(3):220-226. Smith et al. Elife. 2016 Dec 3;5. pii: e21337. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Helen Schmidt and Deepika Calidas Received: 07/09/19 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3553 Species: Caenorhabditis elegans Genotype: meg-3(ax4503[del(1-544)::OLLAS]) meg-4(ax4504) X. Description: Description: 20% maternal effect sterility on average, RNAi insensitivity, MEG-3 does not form cytoplasmic gradient. meg-3(ax4503) is an in-frame deletion in the endogenous meg-3 locus removing amino acids (1-544); MEG-3 does not form a cytoplasmic gradient but does still form granules in early embryos and co-localizes with PGL-3. References: Smith, J. et al. Elife. 2016 Dec 3;5:e21337. doi: 10.7554/eLife.21337. PMID: 27914198 Schmidt H, bioRxiv 2020.10.15.340570 (2020) doi:10.1101/2020.10.15.340570. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Helen Schmidt Received: 05/18/21 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3562 Species: C. elegans Genotype: gtbp-1(ax5000[gtbp-1::tagRFP]) IV; meg-3(ax3054[meg-3::meGFP]) X. Description: tagRFP tag inserted into endogenous gtbp-1 locus. meGFP tag inserted between P121 and V122 of endogenous MEG-3. Reference: Lee, CYS, et al. Elife. 020 Jan 24:9:e52896. doi: 10.7554/eLife.52896. PMID: 31975687. Mutagen: Outcrossed: x0 Made by: Alexandre Paix Received: 10/10/24 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3571 Species: Caenorhabditis elegans Genotype: mut-16(ax4313[mut-16::meGFP]) I. Description: meGFP tag inserted at C-terminus of endogenous mut-16 locus. Reference: Thomas, Bodas, and Seydoux (2025). "FG repeats drive co-clustering of nuclear pores and P granules in the C. elegans germline." Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: John Paul Ouyang Received: 02/26/25 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3614 Species: Caenorhabditis elegans Genotype: par-1(ax4202[par-1(T983A)]) V/nT1[qIs51] (IV;V); meg-3(ax3054[meg-3::meGFP]) X. Description: meGFP inserted between P121 and V122 of endogenous MEG-3. qIs51 [myo-2p::GFP + pes-10p::GFP + F22B7.9p::GFP]. Heterozygotes are wild-type GFP+ and segregate non-GFP par-1 homozygotes (viable, lay dead embryos), wild-type GFP+ heterozygotes, and arrested nT1[qIs51] aneuploids. Pick wild-type GFP+ and check for correct segregation of progeny to maintain. Replacement of threonine 983 with alanine eliminates PAR-1 asymmetry. Reference: Folkman AW & Seydoux G. Development. 2019 Mar 25;146(6):dev171116. doi: 10.1242/dev.171116. PMID: 30814118 Mutagen: Outcrossed: x0 Made by: Andrew Folkmann Received: 07/11/23 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3616 Species: C. elegans Genotype: par-1(ax4206[par-1::meGFP]) V. Description: meGFP tag inserted at C-terminus of endogenous par-1 locus. Reference: Folkman AW & Seydoux G. Development. 2019 Mar 25;146(6):dev171116. doi: 10.1242/dev.171116. PMID: 30814118 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Andrew Folkmann Received: 03/15/24 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3619 Species: C.elegans Genotype: par-1(ax4208[meGFP::delta-KA1]) V/nT1[qIs51] (IV;V). Description: qIs51 [myo-2p::GFP + pes-10p::GFP + F22B7.9p::GFP]. Heterozygotes are wild-type GFP+ and segregate non-GFP par-1 homozygotes (viable, lay viable progeny that are completely sterile), wild-type GFP+ heterozygotes, and arrested nT1[qIs51] aneuploids. Pick wild-type GFP+ and check for correct segregation of progeny to maintain. par-1(ax4208) removes the KA1 domain from a GFP-tagged version of PAR-1. Reference: Folkman AW & Seydoux G. Development. 2019 Mar 25;146(6):dev171116. doi: 10.1242/dev.171116. PMID: 30814118 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Andrew Folkmann Received: 03/15/24 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3644 Species: Caenorhabditis elegans Genotype: fog-2(q71) V; meg-3(ax4320[meg-3::mCherry]) X. Description: mCherry inserted at C terminus of endogenous meg-3 locus. fog-2(q71) is male/female strain. Maintain by mating. XX animals are female. XO animals are WT males. Reference: Putnam A, et al. Nat Struct Mol Biol. 2019 Mar;26(3):220-226. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Madeline Cassani Received: 07/09/19 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3657 Species: Caenorhabditis elegans Genotype: pgl-3(ax4300[pgl-3::mCherry]) fog-2(q71) V. Description: mCherry inserted at C terminus of endogenous pgl-3 locus. fog-2(q71) is male/female strain. Maintain by mating. XX animals are female. XO animals are WT males. Reference: Putnam A, et al. Nat Struct Mol Biol. 2019 Mar;26(3):220-226. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Madeline Cassani Received: 07/09/19 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3678 Species: C. elegans Genotype: mex-5(ax3050[mCherry::mex-5])/nT1[qIs51] IV; par-1(ax4209[par-1(T983A)::meGFP])/nT1[qIs51] V. Description: qIs51 [myo-2p::GFP + pes-10p::GFP + F22B7.9p::GFP]. Heterozygotes are wild-type myo-2::GFP+ and segregate non-myo-2::GFP ax3050; ax4209 homozygotes (maternal effect lethal), wild-type myo-2::GFP+ heterozygotes, and arrested nT1[qIs51] aneuploids. Pick wild-type myo-2::GFP+ and check for correct segregation of progeny to maintain. References: Smith J, et al. eLife. 2016 Dec 3;5:e21337. doi: 10.7554/eLife.21337. PMID: 27914198. Folkman A, et al. Development. 2019 Mar 25;146(6):dev171116. doi: 10.1242/dev.171116. PMID: 30814118. Mutagen: Outcrossed: x0 Made by: Andrew Folkmann Received: 04/04/23 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3679 Species: C. elegans Genotype: mex-5(ax3050[mCherry::mex-5]) IV; par-1(ax4206[par-1::meGFP]) V. Description: qIs51 [myo-2p::GFP + pes-10p::GFP + F22B7.9p::GFP]. Homozygous mex-5(ax3050[mCherry::mex-5]); par-1(ax4206[par-1::meGFP]) animals are fully viable and fertile. References: Smith J, et al. eLife. 2016 Dec 3;5:e21337. doi: 10.7554/eLife.21337. PMID: 27914198. Folkman A, et al. Development. 2019 Mar 25;146(6):dev171116. doi: 10.1242/dev.171116. PMID: 30814118. Mutagen: Outcrossed: x0 Made by: Andrew Folkmann Received: 04/04/23 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3861 Species: Caenorhabditis elegans Genotype: meg-3(ax4502[meg-3(F705A,Y708A,Y713A, N725A)::OLLAS]) meg-4(ax3052) X. Description: 20% Maternal effect sterility on average, embryonic P granule defect, RNAi insensitivity. Engineered mutations in the endogenous meg-3 locus disrupt the binding and localization of PGL proteins to P granules in the early embryo while MEG-3 itself is still forms an asymmetric cytoplasmic gradient and granules. References: Smith, J. et al. Elife. 2016 Dec 3;5:e21337. doi: 10.7554/eLife.21337. PMID: 27914198 Schmidt H, bioRxiv 2020.10.15.340570 (2020) doi:10.1101/2020.10.15.340570. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Helen Schmidt Received: 05/20/21 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3867 Species: C. elegans Genotype: bqSi189 II; npp-10(ax4538[mNeonGreen::npp-10]) III. Description: bqSi189 [lmn-1p::mCherry::his-58::pie-1 3'utr] II. mNeonGreen is inserted at the N-terminus of the endogenous npp-10 locus. bqSi189 is a single-copy MosSCI insertion into ttTi5605. Reference: Thomas et al. 2022. Cytoplasmic nucleoporin foci are stress-sensitive, non-essential condensates in C. elegans Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Laura Thomas Received: 05/12/23 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3872 Species: C. elegans Genotype: npp-14(ax4548[npp-14::OLLAS]) I. Description: OLLAS is inserted at the C-terminus of the endogenous npp-14 locus. Reference: Thomas et al. 2022. Cytoplasmic nucleoporin foci are stress-sensitive, non-essential condensates in C. elegans Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Laura Thomas Received: 05/12/23 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3886 Species: C. elegans Genotype: npp-14(ax4543) I. Description: ax4523 is a deletion residues 24-1387 of the npp-14 coding region. Reference: Thomas et al. 2022. Cytoplasmic nucleoporin foci are stress-sensitive, non-essential condensates in C. elegans Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Laura Thomas Received: 05/12/23 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3904 Species: C. elegans Genotype: tdp-1(ax4546[tdp-1::wrmScarlet]) II. Description: wrmScarlet is inserted at the C-terminus of the endogenous tdp-1 locus. Reference: Thomas et al. 2022. Cytoplasmic nucleoporin foci are stress-sensitive, non-essential condensates in C. elegans Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Laura Thomas Received: 05/12/23 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3906 Species: C. elegans Genotype: ran-2(ax4545[ran-2::wrmScarlet]) III. Description: wrmScarlet is inserted at the C-terminus of the endogenous ran-2 locus. Reference: Thomas et al. 2022. Cytoplasmic nucleoporin foci are stress-sensitive, non-essential condensates in C. elegans Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Laura Thomas Received: 05/12/23 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3938 Species: C. elegans Genotype: bqSi189 II; npp-9(ax4540[mNeonGreen::npp-9]) III. Description: bqSi189 [lmn-1p::mCherry::his-58::pie-1 3'utr] II. mNeonGreen is inserted at the N-terminus of the endogenous npp-9 locus. bqSi189 is a single-copy MosSCI insertion into ttTi5605. Reference: Thomas et al. 2022. Cytoplasmic nucleoporin foci are stress-sensitive, non-essential condensates in C. elegans Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Basma Taleb Ismail Received: 05/12/23 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3944 Species: Caenorhabditis elegans Genotype: npp-12(ax4544) I. Description: ax4544 is a CRISPR-engineered null mutant of npp-12. Reference: Thomas, Bodas, and Seydoux (2025). "FG repeats drive co-clustering of nuclear pores and P granules in the C. elegans germline." Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Basma Taleb Ismail Received: 02/26/25 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH3955 Species: C. elegans Genotype: bqSi189 II; xpo-1(ax4542[xpo-1::mNeonGreen]) V. Description: bqSi189 [lmn-1p::mCherry::his-58::pie-1 3'utr] II. mNeonGreen is inserted at the C-terminus of the endogenous xpo-1 locus. bqSi189 is a single-copy MosSCI insertion into ttTi5605. Reference: Thomas et al. 2022. Cytoplasmic nucleoporin foci are stress-sensitive, non-essential condensates in C. elegans Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Laura Thomas Received: 05/12/23 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH4008 Species: Caenorhabditis elegans Genotype: htp-3 (ax3010[htp-3::TEV::eGFP::myc::3Xflag]) I. Description: Express tagged htp-3. Reference: Paix A, et al. Genetics. 2015 Sep;201(1):47-54. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Alexandre Paix Received: 09/01/16 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH4012 Species: C. elegans Genotype: gtbp-1(ax4561[gtbp-1p::IBB domain::mNeonGreen::gtbp-1 3'utr]) IV. Description: The Importin Beta Binding domain (IBB)::mNeonGreen reporter replaces gtbp-1 in the endogenous gtbp-1 locus. Sterile at 25C; maintain at 20C. Reference: Thomas et al. 2022. Cytoplasmic nucleoporin foci are stress-sensitive, non-essential condensates in C. elegans Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Alex Paix Received: 05/12/23 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH4072 Species: C. elegans Genotype: pgl-3(ax4517[pgl-3::3xFLAG]) V; meg-3(ax3054[meg-3::meGFP]) X. Description: 3xFlag tag inserted at C-terminus of endogenous pgl-3 locus. Inserted into parental strain JH3503 meg-3(ax3054[meg-3::meGFP]). Reference: Ouyang JPT, et al. Nature Cell Biology 2022 (24)1129–1140. DOI: 10.1038/s41556-022-00940-w. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Brennan Danlasky Received: 03/28/23 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH4073 Species: C. elegans Genotype: pgl-3(ax4516[pgl-3(delta448-693)::3xFLAG]) V; meg-3(ax3054[meg-3::meGFP]) X. Description: 3xFlag tag inserted at truncated C-terminus of endogenous pgl-3 locus. Inserted into parental strain JH3503 meg-3(ax3054[meg-3::meGFP]). Reference: Ouyang JPT, et al. Nature Cell Biology 2022 (24)1129–1140. DOI: 10.1038/s41556-022-00940-w. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Brennan Danlasky Received: 03/28/23 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH4201 Species: C. elegans Genotype: npp-11(ax4547[npp-11::wrmScarlet]) I. Description: wrmScarlet is inserted at the C-terminus of the endogenous npp-11 locus. Reference: Thomas et al. 2022. Cytoplasmic nucleoporin foci are stress-sensitive, non-essential condensates in C. elegans Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Basma Taleb Ismail Received: 05/12/23 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH4202 Species: C. elegans Genotype: npp-22(ax4549[npp-22::wrmScarlet]) V. Description: wrmScarlet is inserted at the C-terminus of the endogenous npp-22 locus. Reference: Thomas et al. 2022. Cytoplasmic nucleoporin foci are stress-sensitive, non-essential condensates in C. elegans Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Basma Taleb Ismail Received: 05/12/23 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH4473 Species: Caenorhabditis elegans Genotype: imb-1(syb8052[mNeonGreen::imb-1]) I. Description: mNeonGreen tag inserted at N-terminus of endogenous imb-1 locus. Expressed in all tissues. Reference: Thomas, Bodas, and Seydoux (2025). "FG repeats drive co-clustering of nuclear pores and P granules in the C. elegans germline." Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: SunyBiotech Received: 02/26/25 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH4476 Species: Caenorhabditis elegans Genotype: nucl-1(syb8070[nucl-1::wrmScarlet]) IV. Description: wrmScarlet tag inserted at C-terminus of endogenous nucl-1 locus. Reference: Thomas, Bodas, and Seydoux (2025). "FG repeats drive co-clustering of nuclear pores and P granules in the C. elegans germline." Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: SunyBiotech Received: 02/26/25 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH4484 Species: Caenorhabditis elegans Genotype: npp-11(4585[npp-11::AID*::3xFLAG]) I. Description: AID*::3xFLAG tags inserted at C-terminus of endogenous npp-11 locus. AID* is a minimal AID construct of 44 amino acids (https://academic.oup.com/genetics/article/217/3/iyab006/6104563). Reference: Thomas, Bodas, and Seydoux (2025). "FG repeats drive co-clustering of nuclear pores and P granules in the C. elegans germline." Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Laura Thomas Received: 02/26/25 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JH4504 Species: Caenorhabditis elegans Genotype: glh-1(ax4587[glh-1(del18-236) I. Description: ax4587 is a CRISPR-engineered deletion removing the FGG repeats of GLH-1. Reference: Thomas, Bodas, and Seydoux (2025). "FG repeats drive co-clustering of nuclear pores and P granules in the C. elegans germline." Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Laura Thomas Received: 02/26/25 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: JIM113 Species: Caenorhabditis elegans Genotype: ujIs113 II. Description: ujIs113 [pie-1p::mCherry::H2B::pie-1 3'UTR + nhr-2p::his-24::mCherry::let-858 3'UTR + unc-119(+)]. Reference: Zacharias A, et al. PLoS Genet. 2015 Oct 21;11(10):e1005585. Mutagen: Outcrossed: x2 Made by: E. Preston Received: 03/15/25 University of Pennsylvania, Philadelphia, PA ----------------------------------------------------------------------------- Strain: JIM173 Species: Caenorhabditis elegans Genotype: unc-119(tm4063) III; ujEx173. Description: ujEx173 [ceh-36::TY1::EGFP::3xFLAG + unc-119(+)]. Pick non-Unc to maintain. Reference: Walton T, et al. PLoS Genet. 2015 Mar 4;11(3):e1005003. Mutagen: Outcrossed: x0 Made by: E Preston & T Walton Received: 03/15/25 University of Pennsylvania, Philadelphia, PA ----------------------------------------------------------------------------- Strain: JIM193 Species: Caenorhabditis elegans Genotype: ujIs113 II; ujIs193. Description: ujIs113 [pie-1p::mCherry::H2B::pie-1 3'UTR + nhr-2p::his-24::mCherry::let-858 3'UTR + unc-119(+)] II. ujIs193 [nhr-67::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence in fosmid ID#WRM0633cC01 by recombineering. Expression of transgene confirmed by GFP. Mutagen: n/a Outcrossed: x1 Made by: E. Preston Received: 03/15/25 University of Pennsylvania, Philadelphia, PA ----------------------------------------------------------------------------- Strain: JIM220 Species: Caenorhabditis elegans Genotype: ujIs113 II; unc-30(ok613) IV; ceh-36(ok795) X; ujEx173. Description: ujIs113 [pie-1p::mCherry::H2B::pie-1 3'UTR + nhr-2p::his-24::mCherry::let-858 3'UTR + unc-119(+)] II. ujEx173 [ceh-36::TY1::eGFP::3xFLAG + unc-119(+)]. ujEx173 rescues unc-36, suppressing synthetic lethality in animals carrying the array. Reference: Walton T, et al. PLoS Genet. 2015 Mar 4;11(3):e1005003. Mutagen: n/a Outcrossed: x0 Made by: E. Preston Received: 03/15/25 University of Pennsylvania, Philadelphia, PA ----------------------------------------------------------------------------- Strain: JIM271 Species: Caenorhabditis elegans Genotype: ujIs113 II; stIs10286. Description: ujIs113 [pie-1p::mCherry::H2B::pie-1 3'UTR + nhr-2p::his-24::mCherry::let-858 3'UTR + unc-119(+)]. stIs10286 [nob-1::GFP::unc-54 3'UTR + rol-6(su1006)]. stIs10286 contains 9kb promoter and full nob-1 transcript fused to GFP. Reference: Zhao Z, et al. PLoS Genet. 2010 Sep 2;6(9):e1001089. Mutagen: Outcrossed: x1 Made by: E. Preston Received: 03/15/25 University of Pennsylvania, Philadelphia, PA ----------------------------------------------------------------------------- Strain: JIM356 Species: Caenorhabditis elegans Genotype: ujIs113 II; ujIs153. Description: ujIs113 [pie-1p::mCherry::H2B::pie-1 3'UTR + nhr-2p::his-24::mCherry::let-858 3'UTR + unc-119(+)] II. ujIs153 [ceh-13::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence in fosmid ID#WRM0622c06 by recombineering. Expression of transgene confirmed by GFP. Mutagen: n/a Outcrossed: x1 Made by: E. Preston Received: 03/15/25 University of Pennsylvania, Philadelphia, PA ----------------------------------------------------------------------------- Strain: JIN1375 Species: Caenorhabditis elegans Genotype: hlh-30(tm1978) IV. Description: Superficially wild-type. Grows at standard conditions. Reference: Settembre C, et al. Nat Cell Biol. 2013 Jun;15(6):647-58. Mutagen: Outcrossed: x6 Made by: Mitani & Irazoqui Received: 04/22/16 UMass Medical School, Worcester, MA, USA ----------------------------------------------------------------------------- Strain: JIN1679 Species: Caenorhabditis elegans Genotype: jinEx10. Description: jinEx10 [hlh-30p::hlh-30::GFP + rol-6(su1006)]. Pick Rollers to maintain. hlh-30::GFP expression should be visible at relatively low magnification. Reference: Lapierre LR, et. al. Nat Commun. 2013 Aug 8;4:2267. Mutagen: Outcrossed: x0 Made by: Orane Visvikis Received: 10/08/13 Buck Institute for Research on Aging ----------------------------------------------------------------------------- Strain: JIN810 Species: C. elegans Genotype: agIs26. Description: agIs26 [clec-60p::GFP + myo-2p::mCherry]. mCherry expression in pharynx. GFP expression in posterior gut is upregulated during S. aureus infection and at temperatures >15C. [NOTE: This strain has also been described as AU185 in publications.] Reference: Irazoqui JE, et al. Proc Natl Acad Sci U S A. 2008 Nov 11;105(45):17469-74. (PMID: 18981407) Mutagen: Outcrossed: x0 Made by: Javier Elbio Irazoqui Received: 11/15/19 UMass Medical School, Worcester, MA, USA ----------------------------------------------------------------------------- Strain: JJ1014 Species: Caenorhabditis elegans Genotype: mex-3(zu155) dpy-5(e61)/hT1 I; pos-1(zu148) unc-42(e270)/hT1 V. Description: The double heterozygote is WT. WT will segregate WT, DpyUncs, mid-larval lethal (hT1 homozygotes) and dead eggs. The DpyUncs are homozygous for zu155 and zu148 and will segregate only dead embryos; the dead embryos have excess hypodermis and muscle and lack germ cells and intestine. Mutagen: Outcrossed: x Made by: Russell Hill Received: 07/23/99 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ1057 Species: Caenorhabditis elegans Genotype: pop-1(zu189) dpy-5(e61)/hT1 I; him-5(e1490)/hT1 V. Description: Heterozygotes are WT and segregate WT, Dpys which give only dead eggs, mid-larval lethals (hT1 homozygotes), males and dead eggs. Mutation in pop-1 results in the MS blastomere adopting the fate of the E blastomere. pop-1 mutant embryos have twice the amount of wild type gut and only make anterior pharynx. him-5 is outside the region balanced by hT2. Mutagen: Tc1 (RW7096) Outcrossed: x11 Made by: Rueyling Lin Received: 03/15/96 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ1068 Species: Caenorhabditis elegans Genotype: hmp-2(zu364)/hIn1 [unc-54(h1040)] I. Description: hmp-2(zu364) homozygotes are 99% embryonic or L1 lethal due to a defect in embryonic body elongation; approximately 1% survive to adult stages. Well balanced by hIn1. Maintain by picking WT. Mutagen: EMS Outcrossed: x>2 Made by: Mike Costa Received: 05/14/98 Exelixis Inc., San Francisco, CA ----------------------------------------------------------------------------- Strain: JJ1079 Species: Caenorhabditis elegans Genotype: hmr-1(zu389)/lin-11(n566) unc-75(e950) I. Description: Heterozygotes are WT and segregate WT, Hmr inviable embyros and Egl Unc. Hmr: Hammerhead - defective hypodermal enclosure, especially in anterior regions; approximately 2% of zu389 embryos enclose normally and are Hmp [Humpback: defective body elongation, abnormal bulges on dorsal side]. See also WBPaper00005031. Received new stock from Allison Lynch in the Hardin lab 3/2009. Mutagen: EMS Outcrossed: x>5 Made by: Mike Costa Received: 04/02/99 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ1129 Species: Caenorhabditis elegans Genotype: elt-1(zu180) unc-43(e408)/unc-24(e138) dpy-20(e1282) IV. Description: Heterozygotes are WT and segregate WT, DpyUncs and dead eggs. Mutagen: EMS Outcrossed: x>7 Made by: Jim Priess Received: 03/11/99 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ1136 Species: Caenorhabditis elegans Genotype: unc-119(e2498) III; zuEx24. Description: zuEx24 [hmp-1::GFP + unc-119(+)]. Animals with the duplication are WT. Occasionally pick green hermaphrodites to maintain. zuEx24 transmits at a very high frequency. Mutagen: Outcrossed: x Made by: C Agbunag Received: 06/19/02 University of Wisconsin-Madison, Madison WI, USA ----------------------------------------------------------------------------- Strain: JJ1142 Species: Caenorhabditis elegans Genotype: hmr-1(zu248) I; zuEx2. Description: zuEx2 [W02B9(cosmid) + rol-6(su1006). Maintain by picking Rollers. Mutagen: EMS Outcrossed: x5 Made by: Mike Costa Received: 03/10/08 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ1237 Species: Caenorhabditis elegans Genotype: mex-6(pk440) II. Description: Viable, fertile, apparently normal. Mutagen: Outcrossed: x Made by: Received: 06/05/00 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ1238 Species: Caenorhabditis elegans Genotype: unc-30(e191) mex-5(zu199) IV/nT1 (IV;V). Description: Heterozygotes are WT. Embryos from unc-30 mex-5 homozygotes produce approximately the WT number of cells but do not undergo body morphogenesis and die without hatching. Mutagen: Outcrossed: x Made by: Received: 06/05/00 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ1244 Species: Caenorhabditis elegans Genotype: mex-6(pk440) II; unc-30(e191) mex-5(zu199) IV/nT1 (IV;V). Description: Heterozygotes are WT. Embryos from mex-6; unc-30 mex-5 homozygotes produce approximately the WT number of cells but do not undergo body morphogenesis and die without hatching. Mutagen: Outcrossed: x Made by: Received: 06/05/00 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ1271 Species: Caenorhabditis elegans Genotype: glo-1(zu391) X. Description: Lacks autofluorescent and birefringent gut granules. Mutagen: EMS Outcrossed: x Made by: Greg Hermann Received: 07/05/01 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ1440 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; zuIs20. Description: zuIs20 [par-3p::par-3::ZF1::GFP + unc-119(+)]. GFP-tagged PAR-3 that is degraded in early embryonic somatic cells. Worms carrying the transgene are non-Unc and express GFP very weakly in early embryos (only detectable by antibody staining), and later in adherens junctions of epithelial cells. Mutagen: Microparticle Bombardment Outcrossed: x0 Made by: Jeremy Nance Received: 06/29/06 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ1473 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; zuIs45 V. Description: zuIs45 [nmy-2p::nmy-2::GFP + unc-119(+)] V. NMY-2::GFP is expressed in the germline and somatic gonad. Mutagen: Microparticle Bombardment Outcrossed: x Made by: Ed Munro Received: 01/30/04 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ1494 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; zuIs58. Description: zuIs58 [unc-119(+) + par-6::PAR-6::ZF1::GFP]. Mutagen: Outcrossed: x Made by: Received: 05/24/06 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ1508 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; zuIs60. Description: zuIs60 [pie-1p::GFP(secreted) + unc-119(+)]. Maternally-expressed secreted GFP fills spaces between embryonic cells, and space between embryo and vitelline membrane. Useful marker for vizualizing intercellular spaces in embryos. Reference: Wehman AM, et al. Curr Biol. 2011 Dec 6;21(23):1951-9. Mutagen: Outcrossed: x Made by: Jeremy Nance Received: 03/15/25 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ1549 Species: Caenorhabditis elegans Genotype: efl-1(se1) V. Description: Temperature sensitive. At restrictive temperature (26C), efl-1 produces dead eggs with a Mex phenotype. Two ts periods: 1) prior to L4 results in sterility, 2) after L4 results in a maternal-effect embryonic lethal phenotype. Mutagen: EMS Outcrossed: x7 Made by: D. Waring Received: 03/25/03 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ1550 Species: Caenorhabditis elegans Genotype: dpl-1(zu355) unc-4(e120)/rol-6(e187) let-23(sy97) II. Description: Heterozygotes are WT and segregate WT, Uncs which give only deads egss with a Mex phenotype, and Vulvaless Rollers. sy97 is only 15% viable. Mutagen: EMS Outcrossed: x7 Made by: Barbara Page Received: 03/25/03 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ1578 Species: C. elegans Genotype: par-6(zu222) unc-101(m1); zuIs54. Description: zuIs54 [par-6p::par-6::ZF1::GFP + unc-119(+)]. Unc. GFP-tagged PAR-6 that degrades in early embryonic somatic cells; rescues the Par phenotype of par-6(zu222). Delayed gastrulation of endodermal cells. Reference: Nance J, Munro EM, Priess JR. Development. 2003 Nov;130(22):5339-50. Mutagen: Outcrossed: x1 Made by: Jeremy Nance Received: 05/22/18 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: JJ1600 Species: C. elegans Genotype: par-3(it71) lon-1(e185) III; him-8(e1489) IV; zuIs20. Description: zuIs20 [par-3p::par-3::ZF1::GFP + unc-119(+)]. Lon. Him. GFP-tagged PAR-3 that is degraded in early embryonic somatic cells. Rescues the Par phenotype of par-3(it71). Delayed endodermal cell gastrulation. Reference: Nance J, Munro EM, Priess JR. Development. 2003 Nov;130(22):5339-50. Mutagen: Outcrossed: x1 Made by: Jeremy Nance Received: 05/22/18 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: JJ1743 Species: Caenorhabditis elegans Genotype: par-6(tm1425)/hIn1 [unc-54(h1040)] I; him-8(e1489) IV. Description: Heterozygotes are WT and segregate WT, Uncs, dead larvae (par-6 homozygotes) and males. Mutagen: Outcrossed: x Made by: Jeremy Nance Received: 04/05/07 University of Wisconsin, Madison, WI ----------------------------------------------------------------------------- Strain: JJ1833 Species: Caenorhabditis elegans Genotype: dpy-5(e61) I; unc-32(e189) III. Description: Dpy Unc. Mutagen: Outcrossed: x Made by: Jesse Goldmark Received: 03/23/06 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ185 Species: Caenorhabditis elegans Genotype: dpy-13(e184) skn-1(zu67) IV; mDp1 (IV;f). Description: Animals with the duplication are WT. Animals which have lost the duplication are Dpy and give only dead eggs. Mutagen: Outcrossed: x Made by: Received: 04/08/04 University of Oregon, Eugene ----------------------------------------------------------------------------- Strain: JJ1850 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; zuIs178. Description: zuIs178 [his-72(1kb 5' UTR)::his-72::SRPVAT::GFP::his-72 (1KB 3' UTR) + 5.7 kb XbaI - HindIII unc-119(+)]. HIS-72::GFP can be detected in germline and most somatic nuclei, but not in intestinal nuclei. Not integrated on X. Mutagen: Outcrossed: x Made by: Siew-Loon Ooi Received: 12/02/08 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ1851 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; zuEx181. Description: zuEx181[his-72(1kb 5' UTR)::YFP::GSRPVAT::HIS-72::HIS-72 (1KB 3'UTR) + 5.7 kb XbaI - HindIII UNC-119(+)]. HIS-72::YFP signal can be detected in the germline and most somatic nuclei, but not in intestinal nuclei. Mutagen: Outcrossed: x Made by: Siew-Loon Ooi Received: 12/02/08 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ1852 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; zuEx182. Description: zuEx182[his-71(1kb 5' UTR):: HIS-71::GSRPVAT::GFP::::HIS-71 (1KB 3'UTR) + 5.7 kb XbaI - HindIII UNC-119(+)]. HIS-71::YFP signal can be detected in most somatic nuclei, including the intestinal nuclei, but is not detected in the germline. Mutagen: Outcrossed: x Made by: Siew-Loon Ooi Received: 12/02/08 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ1972 Species: Caenorhabditis elegans Genotype: eel-1(zu462) unc-33(e204) IV. Description: Slow growth and a maternal-effect enhancer of the efl-1(se1) embyronic lethal phenotype. Unc. Mutagen: EMS Outcrossed: x>7 Made by: Barbara Page Received: 08/17/07 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ1992 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; zuIs145. Description: zuIs145 [nmy-2p::mom::GFP + unc-119(+)]. Wild type phenotype. Mutagen: Outcrossed: x4 Made by: Fred Park Received: 05/07/08 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ2059 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; zuIs235. Description: zuIs235 [his-72(1-kb 5' UTR):: BIOTAG::3XHA::HIS-72::his-72(1-kb 3' UTR) + unc-119(+)]. Superficially wild-type. Mutagen: Outcrossed: x0 Made by: Siew-Loon Ooi Received: 01/12/10 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ2060 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; zuIs236. Description: zuIs236 [his-72(1-kb 5' UTR)::BIRA::GFP:: his-72(1-kb 3' UTR) + unc-119(+)]. Superficially wild-type. Mutagen: Outcrossed: x0 Made by: Siew-Loon Ooi Received: 01/12/10 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ2061 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; zuIs235; zuIs236. Description: zuIs235 [his-72(1-kb 5' UTR):: BIOTAG::3XHA::HIS-72::his-72(1-kb 3' UTR) + unc-119(+)]. zuIs236 [his-72(1-kb 5' UTR)::BIRA::GFP:: his-72(1-kb 3' UTR) + unc-119(+)]. Superficially wild-type. Mutagen: Outcrossed: x0 Made by: Siew-Loon Ooi Received: 01/12/10 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ2281 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; zuIs258. Description: zuIs258 [his-72p::his-72(5' UTR)::BIRA::GFP::his-72(3' UTR) + unc-119(+)]. GFP expression detectable in embryos. Reference: Steiner FA, et al. Genome Res. 2012 Apr;22(4):766-77. Mutagen: Bombardment Outcrossed: x0 Made by: Florian Steiner Received: 04/22/13 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ2286 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; zuIs263. Description: zuIs263 [myo-3p::myo-3(5' UTR)::npp-9::mCherry::BLRP::3xFLAG::npp-9(3' UTR) + unc-119(+)]. Reference: Steiner FA, et al. Genome Res. 2012 Apr;22(4):766-77. Mutagen: Bombardment Outcrossed: x0 Made by: Florian Steiner Received: 04/22/13 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ2300 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; zuIs258; zuIs263. Description: zuIs258 [his-72p::his-72(5' UTR)::BIRA::GFP::his-72(3' UTR) + unc-119(+)]. GFP expression detectable in embryos. zuIs263 [myo-3p::myo-3(5' UTR)::npp-9::mCherry::BLRP::3xFLAG::npp-9(3' UTR) + unc-119(+)]. Reference: Steiner FA, et al. Genome Res. 2012 Apr;22(4):766-77. Mutagen: Bombardment Outcrossed: x0 Made by: Florian Steiner Received: 04/22/13 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ2586 Species: Caenorhabditis elegans Genotype: cox-4(zu476[cox-4::eGFP::3xFLAG]) I. Description: cox-4(zu476[cox-4::eGFP::3xFLAG]) I. Endogenous cox-4 locus tagged with eGFP via genome editing. Mitochondria in all cell types are labeled with GFP. Reference: Raiders SA, et al. PLoS Genet. 2018 Jul 19;14(7):e1007417. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Michael Eastwood Received: 10/25/18 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ462 Species: Caenorhabditis elegans Genotype: +/nT1 IV; pos-1(zu148) unc-42(e270)/nT1 V. Description: Heterozygotes are WT and segregate WT, Uncs, Vul and dead eggs. Uncs are homozygous for zu148 and will segregate only dead embryos; the dead embryos will have no morphogenesis and will lack germ cells and intestine. Mutagen: EMS Outcrossed: x Made by: Craig Mello Received: 07/23/99 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ478 Species: Caenorhabditis elegans Genotype: mex-3(zu155) egl-30(n686)/hT1 I; +/hT1 V. Description: Heterozygotes are WT and segregate WT, Egls which give only dead eggs, dead eggs, and mid-larval lethals (hT1 homozygotes) Mutagen: Outcrossed: x Made by: Received: 10/15/96 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ529 Species: Caenorhabditis elegans Genotype: rol-1(e91) mex-1(zu121)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUnc and Rollers. Rollers give only dead eggs. Maintain by picking WT. mex-1 is to the right of rol-1, is uncovered by mnDf87 and is allelic to mel-21. Mutagen: EMS Outcrossed: x8 Made by: Received: 08/09/93 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ532 Species: Caenorhabditis elegans Genotype: pie-1(zu154) unc-25(e156)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, Unc and DpySteriles. Uncs give only dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x7 Made by: Received: 08/09/93 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ746 Species: Caenorhabditis elegans Genotype: +/nT1 IV; apx-1(zu183) dpy-11(e224)/nT1 V. Description: Heterozygotes are WT and segregate WT, Dpy, Vul and dead eggs. The Dpys give only dead eggs. apx-1 is a maternal effect lethal. zu183 is recessive. Mutagen: Spontaneous in RW7096. Outcrossed: x5 Made by: Craig Mello Received: 07/25/94 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ862 Species: Caenorhabditis elegans Genotype: hmp-1(zu278)/daf-11(m84) sma-1(e30) V. Description: Heterozygotes are WT and segregate WT, Hmp inviable embyros (Hmp: humpback-defective body elongation, abnormal bulges on dorsal side) and Daf Sma (ts Daf). Mutagen: EMS Outcrossed: x>5 Made by: Mike Costa Received: 04/02/99 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JJ938 Species: Caenorhabditis elegans Genotype: unc-24(e138) daf-14(m77)/elt-1(zu180) dpy-20(e1282) IV. Description: At 20C or 25C, heterozygotes are WT and segregate WT, UncDaf and dead eggs. At 15C, heterozygotes are WT and segregate WT, Uncs and dead eggs. Mutagen: EMS Outcrossed: x>7 Made by: Jim Priess Received: 03/11/99 Fred Hutchinson Cancer Research Center, Seattle, WA ----------------------------------------------------------------------------- Strain: JK1009 Species: Caenorhabditis elegans Genotype: fog-1(q180)/sup-11(n403) unc-11(e47) I. Description: Heterozygotes are WT and segregate WT, small scrawny slow-growing Uncs, and females. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 04/07/95 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1019 Species: Caenorhabditis elegans Genotype: dpy-19(e1259) glp-1(q158) III; qDp3 (III;f). Description: Animals with the Duplication are WT. Animals which have lost the Duplication are Dpy and Sterile. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 03/16/90 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1058 Species: C. elegans Genotype: gld-1(q343)/unc-11(e47) dpy-5(e61) I Description: Heterozygotes are WT and segregate WT, Dpy Uncs, and homozygous q343 (make small abnormal oocytes. Pick WT and check for correct segregation of progeny to maintain. Reference: Francis R, et al. Genetics. 1995 Feb; 139(2): 579–606. doi: 10.1093/genetics/139.2.579 PMID: 7713419. Mutagen: mut-2 Outcrossed: x0 Made by: Judith Kimble Received: 09/29/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1075 Species: Caenorhabditis elegans Genotype: spe-4(q347)/unc-11(e47) dpy-5(e61) I. Description: Heterozygotes are WT. Segregates Dpy Uncs, and females (primary spermatocyte death). Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: mut-2 Outcrossed: x Made by: Received: 02/18/08 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1107 Species: Caenorhabditis elegans Genotype: glp-1(q224) III. Description: Temperature sensitive - raise at 15C. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x>1 Made by: Received: 05/11/10 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1122 Species: Caenorhabditis elegans Genotype: dpy-17(e164) unc-32(e189)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, DpyUncs and DpySteriles. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 07/03/97 MIT, Cambridge MA, USA ----------------------------------------------------------------------------- Strain: JK1129 Species: Caenorhabditis elegans Genotype: unc-32(e189) qDf2/eT1 III; +/eT1 V. Description: Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 10/13/94 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1153 Species: Caenorhabditis elegans Genotype: fog-1(q372) ace-2(g202) dpy-5(e61)/unc-11(e47) I. Description: Heterozygotes are WT and segregate WT, Dpy Fogs, and Uncs. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 04/20/06 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1195 Species: Caenorhabditis elegans Genotype: lin-12(q269) glp-1(q231)/unc-32(e189) III. Description: Heterozygotes are WT and segregate WT, Uncs, and Lags (ts). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 07/01/05 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1219 Species: Caenorhabditis elegans Genotype: unc-34(e315) lag-2(q387) V/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc. [Note from Kimble lab: Min Han did a complementation test on this strain and found that unc-34(e315) is lost from this strain.] Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 10/10/97 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1223 Species: Caenorhabditis elegans Genotype: lag-2(q411) V/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc. [Used to be listed in Kimble lab as unc-5(e53); lag-2(q411 dpy-11(e224)/DnT1. Needs to be checked.] Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Eric Lambie Received: 10/10/97 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1227 Species: Caenorhabditis elegans Genotype: lag-1(q385)/dpy-13(e184) unc-24(e138) IV. Description: Heterozygotes are WT and segregate WT, DpyUnc and early larval lethals. Received new stock 5/98. Check carefully for segregation of Dpy Unc progeny; dpy-13 can be lost easily. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x Made by: E. Lambie Received: 11/29/93 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1277 Species: Caenorhabditis elegans Genotype: lag-2(q420) V. Description: Temperature sensitive. Maintain at 15C. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 11/03/97 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1313 Species: Caenorhabditis elegans Genotype: lin-12(n941) glp-1(q46)/dpy-19(e1259) unc-69(e587) III. Description: Heterozygotes are WT and segregate WT, Dpy(ts) Uncs, and Lag L1 lethals (not ts). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 07/01/05 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1346 Species: Caenorhabditis elegans Genotype: cyn-4(q465)/dpy-10(e128) unc-4(e120) II. Description: Heterozygotes are WT and segregate WT, DpyUnc and sterile hermaphrodites. Maintain by picking fertile WT hermaphrodites. q465 previously called mog-6. cyn-4 previously called cyp-4. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Received: 10/13/94 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1389 Species: Caenorhabditis elegans Genotype: mog-1(q223) unc-69(e587)/eT1 III; +/eT1 V. Description: Heterozygotes are wild-type and segregate wild-type, Unc-36 (eT1 homozygotes), homozygous Mog (Unc, weak coiler). Maintain by picking wild-type. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Received: 10/13/94 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1403 Species: Caenorhabditis elegans Genotype: fog-3(q470)/unc-13(e1091) lin-11(n566) I. Description: Heterozygotes are WT and segregate WT, Unc Egls and females. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Ron Ellis Received: 10/16/97 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1438 Species: Caenorhabditis elegans Genotype: daf-2(m65)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, non-conditional dauers, and Sterile Dpys. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 03/24/09 University College, London, England ----------------------------------------------------------------------------- Strain: JK1443 Species: Caenorhabditis elegans Genotype: lag-1(q476) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: S. Christensen Received: 06/14/07 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1466 Species: Caenorhabditis elegans Genotype: gld-1(q485)/dpy-5(e61) unc-13(e51) I. Description: Heterozygotes are WT and segregate WT, DpyUncs and steriles with a tumorous germline. Pick WT to maintain. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 02/05/98 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1499 Species: Caenorhabditis elegans Genotype: mog-2(q75)/sqt-1(e1350) II; him-8(e1489) IV. Description: At 15C heterozygotes are Rollers and segregate Rollers, Sqt and Steriles. Not a healthy strain; recombination occurs, too. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Received: 10/13/94 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1505 Species: Caenorhabditis elegans Genotype: unc-32(e189) glp-1(e2072) III/eT1 (III;V). Description: Heterozygotes are WT and segregate WT, Sterile Unc coilers, Unc-36s (eT1 homozygotes) and dead eggs. Pick WT to maintain. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 04/03/96 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1521 Species: Caenorhabditis elegans Genotype: fog-2(q71) pha-4(q490)/stu-3(q265) rol-9(sc148) V. Description: Heterozygotes are WT and segregate WT, Females which lack a pharynx (arrest as embryos or L1) and Sterile Unc Rollers. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. See also WBPaper00003770. Mutagen: Outcrossed: x Made by: S. Mango Received: 04/07/95 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1534 Species: Caenorhabditis elegans Genotype: ces-1(n703) qDf5/unc-29(e193) mec-8(e398) blmp-1(s71) I. Description: Heterozygotes are Ces and grow slowly. Hets segregate DpyUncs and dead eggs. ces-1(n703) is dominant. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: UV/TMP Outcrossed: x Made by: Ron Ellis Received: 03/22/95 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1542 Species: Caenorhabditis elegans Genotype: ces-1(n703) qDf7/dpy-5(e61) srf-2(yj262) unc-75(e950) I. Description: Heterozygotes are Srf and grow slowly. Hets segregate Srf, DpyUncSrf and dead eggs. ces-1(n703) is dominant. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. CGC rec'd new stock 10/99. Mutagen: UV/TMP Outcrossed: x Made by: Ron Ellis Received: 04/07/95 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1545 Species: Caenorhabditis elegans Genotype: ces-1(n703) qDf8/unc-13(e1091) lin-11(n566) I. Description: Heterozygotes are Ces and segregate VulUncs and dead eggs. ces-1(n703) is dominant. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: UV/TMP Outcrossed: x Made by: Ron Ellis Received: 03/22/95 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1547 Species: Caenorhabditis elegans Genotype: ces-1(n703) qDf10/unc-13(e1091) lin-11(n566) I. Description: Heterozgotes are Ces and segregate VulUncs and dead eggs. ces-1(n703) is dominant. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: UV/TMP Outcrossed: x Made by: Ron Ellis Received: 03/22/95 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1548 Species: Caenorhabditis elegans Genotype: ces-1(n703) qDf11/unc-13(e1091) lin-11(n566) I. Description: Heterozygotes are Ces and segregate Ces, UncVul and dead eggs. ces-1(n703) is dominant. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: UV/TMP Outcrossed: x Made by: Ron Ellis Received: 04/07/95 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1550 Species: Caenorhabditis elegans Genotype: ces-1(n703) qDf12/unc-13(e1091) lin-11(n566) I. Description: Heterozygotes are Ces and segregate VulUnc and dead eggs. ces-1(n703) is dominant. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: UV/TMP Outcrossed: x Made by: Ron Ellis Received: 03/22/95 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1553 Species: Caenorhabditis elegans Genotype: ces-1(n703) qDf9/unc-29(e1072) lin-11(n566) I. Description: Heterozygotes are Ces and throw dead eggs and Unc Vuls. ces-1(n703) is dominant. Well balanced. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Ron Ellis Received: 03/26/96 Rowan University, Stratford, NJ ----------------------------------------------------------------------------- Strain: JK1556 Species: Caenorhabditis elegans Genotype: ces-1(n703) qDf14/unc-13(e1091) lin-11(n566) I. Description: Heterozygotes are Ces and segregate Ces, UncVul and dead eggs. ces-1(n703) is dominant. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x6 Made by: Ron Ellis Received: 04/07/95 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1557 Species: Caenorhabditis elegans Genotype: ces-1(n703) qDf13/unc-13(e1091) lin-11(n566) I. Description: Heterozygotes are Ces and segregate VulUncs and dead eggs. ces-1(n703) is dominant. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x5 Made by: Ron Ellis Received: 03/22/95 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1561 Species: Caenorhabditis elegans Genotype: ces-1(n703) qDf15/unc-29(e193) mec-8(e398) lin-11(n566) I. Description: Heterozygotes are Ces and segregate VulUncs and dead eggs. ces-1(n703) is dominant. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x Made by: Ron Ellis Received: 03/22/95 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1573 Species: Caenorhabditis elegans Genotype: ces-1(n703) qDf6/dpy-14(e188) unc-13(e51) I. Description: Heterozygotes are Ces and grow very slowly and are often sterile. They segregate very early larval lethals, sickly DpyUncs and more hets. ces-1(n703) is dominant. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: UV/TMP Outcrossed: x Made by: Ron Ellis Received: 03/26/96 Rowan University, Stratford, NJ ----------------------------------------------------------------------------- Strain: JK1726 Species: Caenorhabditis elegans Genotype: qDf16/dpy-5(e61) unc-15(e1402) I. Description: Heterozygotes are WT and segregate WT, dead eggs and DpyUncs. Maintain by picking WT. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: X-rays Outcrossed: x3 Made by: Lisa Kadyk Received: 11/20/95 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1743 Species: Caenorhabditis elegans Genotype: gld-2(q497)/dpy-5(e61) unc-13(e51) I. Description: Heterozygotes are WT and segregate WT, DpyUncs and Steriles. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Lisa Kadyk Received: 08/26/98 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1774 Species: Caenorhabditis elegans Genotype: eef-1A.1(q145)/sma-3(e491) unc-36(e251) III. Description: Heterozygotes are WT and segregate WT, SmaUnc and Steriles. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be used for any commercial purpose or for work on human subjects. eft-3(q145) previously called glp-3(q145). Mutagen: Outcrossed: x6 Made by: Lisa Kadyk Received: 04/30/97 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK1973 Species: Caenorhabditis elegans Genotype: fem-3(q96) IV. Description: Semi-dominant Mog. Temperature-sensitive sterile. Maintain at 15C. Masculization of germline (Mog) at 25C. Mutagen: Outcrossed: x Made by: M. Gallegos Received: 07/21/15 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2009 Species: Caenorhabditis elegans Genotype: gon-4(q519) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Uncs, dead eggs and Steriles with a small white patch. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Sonia Santa Anna Received: 02/16/01 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2049 Species: Caenorhabditis elegans Genotype: qIs19 V. Description: qIs19 [lag-2p::GFP::unc-54 3'UTR + rol-6(su1006)] V. Rollers. Integration of qEx233. GFP expression in DTCs and other defined regions. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Integrated with gamma rays Outcrossed: x0 Made by: D. Gao Received: 10/05/01 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2157 Species: C. elegans Genotype: lag-2(q411) V; qEx319. Description: qEx319 [lag-2::cMyc + rol-6(su1006)].  Rollers.  Extrachromosomal array carrying myc-tagged LAG-2 protein partially rescues lag-2(null).  About 70% of progeny are Lag.  Survivors are Rollers.  Reference: Crittenden et al., Mol Biol Cell. 2006 Jul;17(7):3051-61. Mutagen: Outcrossed: x0 Made by: Dali Gao Received: 07/13/17 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2176 Species: Caenorhabditis elegans Genotype: sys-1(q544)/qIs23 I. Description: qIs23 [lag-2::GFP]. Heterozygous. Segregates fertile and rolling heterozygotes, sterile and non-rolling sys-1 homozygotes, and dead qIs23 homozygotes. Maintain by picking rollers. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Gamma radiation Outcrossed: x5 Made by: J. Bork Received: 03/02/10 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2208 Species: Caenorhabditis elegans Genotype: gld-2(dx40)/dpy-5(e61) unc-13(e51) I. Description: Segregates wild-type hets, Ste dx40 homozygotes, and Dpy Uncs. Maintain by picking wild-type and checking for correct segregation. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x5 Made by: L. Kadyk Received: 05/11/10 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2321 Species: Caenorhabditis elegans Genotype: mog-5(q449) unc-4(e120)/mIn1 [dpy-10(e128)] II. Description: Heterozygotes are WT and segregate WT, Dpys, and Uncs which make excess sperm, are elongated and tend to coil, are slow and have poor backing. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x Made by: A. Puoti Received: 07/24/00 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2505 Species: C. elegans Genotype: cyd-1(q626) II; him-5(e1490) V. Description: Temperature-sensitive allele of cyd-1. Phenotypically wild-type at 15C. At 25C, approximately one-third of q626 hermaphrodites were missing one distal tip cell (DTC) and approximately one-half of q626 males were missing the linker cell (LC). q626 also feminizes the XO gonad. q626 affects the production of SGP daughters in both sexes. There is also a maternal component since the mutant phenotype is almost fully penetrant in offspring of homozygous mothers, but less penetrant in offspring of heterozygous mothers. Reference: Tilmann C & Kimble J. Dev Cell. 2005 Oct;9(4):489-99. Mutagen: EMS Outcrossed: x1 Made by: Finn Hugo Markussen Received: 03/18/20 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2532 Species: Caenorhabditis elegans Genotype: gon-1(q518) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Unc, sterile animals with a white patch, and dead eggs. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: R Blelloch Received: 11/08/02 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2533 Species: Caenorhabditis elegans Genotype: qC1 [dpy-19(e1259) glp-1(q339) qIs26] III/eT1 (III;V). Description: qIs26 [lag-2::GFP + rol-6(su1006)]. Throws heterozygous Rollers and Unc eT1 homozygotes. qIs26 was integrated into qC1 and in the process made qC1 homozygous lethal. The distal tip cells are GFP+. It was an integration of qEx233. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: F.H. Markussen Received: 02/16/01 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2589 Species: Caenorhabditis elegans Genotype: nos-3(q650) II. Description: Grows well as a homozgyote. 0.3% sterile at 20C (0.2% have masculinized germ lines). Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: UV/TMP Outcrossed: x8 Made by: Received: 03/09/00 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2663 Species: Caenorhabditis elegans Genotype: dpy-11(e224) mes-4(bn67) V/nT1 [unc-?(n754) let-? qIs50] (IV;V). Description: qIs50 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter (F22B7.9) driving GFP in the intestine. Segregates non-glowing Dpys that are Mes (produce only sterile progeny) and glowing Unc heterozygotes. nT1[unc-?(n754) let-? qIs50] is also known as DnT1[qIs50]. qIs50 is apparently inserted on DnT1. qIs50 is somewhat dimmer than the similar qIs51. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x3 Made by: F-H. Markussen Received: 07/30/99 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2689 Species: Caenorhabditis elegans Genotype: pop-1(q645) dpy-5(e61) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT and glow, segregate non-glowing Dpy Steriles. qIs48 is an insertion of ccEx9747 (carries myo-2::GFP, pes-10::GFP, and a gut enhancer fused to GFP) onto the hT2 chromosome and is homozygous lethal. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Mutagen: Outcrossed: x Made by: K. Siegfried Received: 12/03/01 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2729 Species: Caenorhabditis elegans Genotype: dpy-18(ok162) III. Description: Medium Dpy. Robert Barstead deletion mutant in T28D6. Prolyl-4-hydroxylase alpha subunit. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x4 Made by: Received: 07/24/00 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2735 Species: Caenorhabditis elegans Genotype: qIs54 X. Description: qIs54 [pes-10p::GFP + myo-2p::GFP + F22B7.9::GFP]. Superficially wild-type. GFP expression in pharynx, gut and early embryos. qIs54 males mate, but not as well as wild-type. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x10 Made by: F-H. Markussen Received: 07/30/99 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2736 Species: Caenorhabditis elegans Genotype: ceh-22(q632) V. Description: Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: M. Chesney Received: 03/01/12 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2739 Species: Caenorhabditis elegans Genotype: mcm-4(e1466) dpy-5(e61) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: qIs48 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter driving GFP in the intestine, and is homozygous lethal. Segregates WT glowing hets, non-glowing sterile Dpys, very rare homozygous hT2 glowing animals, and dead eggs. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Mutagen: Outcrossed: x11 Made by: K. Siegfried Received: 07/30/99 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2751 Species: Caenorhabditis elegans Genotype: sys-1(q544) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT. q544 is homozygous embryonic lethal. hT2[qIs48] homozygotes are inviable. PIck GFP+ wild-type to maintain. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: K. Siegfried Received: 07/24/13 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2757 Species: Caenorhabditis elegans Genotype: phy-2(ok177) IV. Description: WT. Deletion mutant in F35G2.4. Prolyl 4 hydroxylase #2 deletion. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 07/24/00 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2761 Species: Caenorhabditis elegans Genotype: sys-1(q544)/unc-29(e1072) fog-3(q470) I. Description: Heterozygotes are WT and segregate WT, Unc Fogs and Sterile Spfs (white patch) phenotype. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: J. Miskowski Received: 05/16/01 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2782 Species: Caenorhabditis elegans Genotype: pop-1(q645) unc-11(e47) I/hT2 (I;III). Description: Heterozygotes are WT and segregate WT, Dpys (hT2 homozygotes) and Unc Steriles. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: K. Siegfried Received: 12/03/01 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2785 Species: Caenorhabditis elegans Genotype: unc-4(e120) II; qIs55. Description: qIs55 [hnd-1::GFP + unc-4(+)] integrated on IV or V. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: 0.1% MMS Outcrossed: x15 Made by: Laura Mathies Received: 03/02/10 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2810 Species: Caenorhabditis elegans Genotype: mcm-4(e1466) dpy-5(e61)/hT2 I; dpy-18(e364) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are medium Dpy and glowing. Hets throw small, sterile DpyUncs and dead eggs. hT2[bli-4(e937) qIs48] is homozygous lethal. qIs48 is an insertion of ccEx9747 with markers myo-2::GFP expressed in the pharynx throughout development, pes-10::GFP expressed in the embyro, and gut promoter F22B7.9 driving GFP in the intestine. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Mutagen: Outcrossed: x20 Made by: K. Siegfried Received: 01/19/01 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2868 Species: Caenorhabditis elegans Genotype: qIs56 V. Description: qIs56 [lag-2p::GFP + unc-119(+)]. Non-unc; might still have unc-119(ed3) in background. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x2 Made by: L. Mathies Received: 10/05/01 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2878 Species: Caenorhabditis elegans Genotype: fog-1(q325) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Segregates WT green-glowing heterozygotes and non-glowing Fogs. qIs48 is an insertion of ccEx9747 (carries myo-2::GFP, pes-10::GFP, and a gut enhancer fused to GFP) onto the hT2 chromosome and is homozygous lethal. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Mutagen: Outcrossed: x Made by: S. Crittenden Received: 04/20/06 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2879 Species: Caenorhabditis elegans Genotype: gld-2(q497) gld-1(q485)/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Segregates WT GFP+ heterozygotes, non-GFP sterile gld-2 gld-1 homozygotes, very rare GFP+ homozygous hT2, and dead eggs. Maintain by picking wild-type GFP+. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: S. Crittenden Received: 11/28/12 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK289 Species: Caenorhabditis elegans Genotype: glp-1(e2142) III. Description: Temperature sensitive. Maintain at 15C. Mutagen: Outcrossed: x Made by: S. Crittenden Received: 07/01/16 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2906 Species: Caenorhabditis elegans Genotype: mep-1(q660) IV/nT1 [qIs51] (IV;V). Description: M04B2.1 Throws Steriles and GFP(+) nT1 heterozygotes and dead eggs. The nT1 chromosome is apparently homozygous lethal since Vul worms are not seen. For some reason, crosses with this nT1[qIs51] chromosome generate an excess of male progeny. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 07/02/02 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2933 Species: Caenorhabditis elegans Genotype: gon-14(q12) V/nT1 [qIs51] (IV;V). Description: Heterozygotes are WT and GFP+. q12 homozygotes have a white-patch phenotype. nT1[qIs51] is probably homozygous lethal. qIs51 is an insertion of ccEx9747 with markers: myo-2::GFP expressed in the pharynx throughout development, pes-10::GFP expressed in the embryo, and a gut promoter F22B7.9::GFP expressed in the intestine. Crosses with this strain generate very few glowing hermaphrodite cross progeny and many glowing male cross progeny. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x Made by: T. Kidd Received: 04/27/04 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2944 Species: Caenorhabditis elegans Genotype: pop-1(q645) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Segregates WT green-glowing heterozygotes and non-glowing steriles. qIs48 is an insertion of ccEx9747 (carries myo-2::GFP, pes-10::GFP, and a gut enhancer fused to GFP) onto the hT2 chromosome and is homozygous lethal. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Mutagen: Outcrossed: x10 Made by: K. Siegfried Received: 12/03/01 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2945 Species: Caenorhabditis elegans Genotype: pop-1(q624) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: q624 has variable gonad defects and can be maintained as a homozygote. qIs48 is an insertion of ccEx9747 (carries myo-2::GFP, pes-10::GFP, and a gut enhancer fused to GFP) onto the hT2 chromosome and is homozygous lethal. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Mutagen: Outcrossed: x10 Made by: K. Siegfried Received: 12/03/01 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2958 Species: Caenorhabditis elegans Genotype: nT1 [qIs51] (IV;V)/dpy-11(e224) unc-42(e270) V. Description: Segregates glowing heterozygotes and DpyUncs. nT1[qIs51] is probably homozygous lethal. qIs51 is an insertion of ccEx9747 with markers: myo-2::GFP expressed in the pharynx throughout development, pes-10::GFP expressed in the embryo, and a gut promoter F22B7.9::GFP expressed in the intestine. Crosses with this strain generate very few glowing hermaphrodite cross progeny and many glowing male cross progeny. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: K. Siegfried Received: 02/16/01 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK2979 Species: Caenorhabditis elegans Genotype: fbf-1(ok224) II. Description: Homozygous. No obvious phenotype by dissecting scope. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: UV/TMP Outcrossed: x8 Made by: Received: 03/25/05 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3022 Species: Caenorhabditis elegans Genotype: fbf-1(ok91) II. Description: Low percent sterile, more sperm than WT, delayed oogenesis, larger broods than WT. PCR amplify: WT band is about 3 kb, deletion band is about 1.2 kb. Oligos used: outer: 201A and 202S; inner: 203A and 204S. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: UV/TMP Outcrossed: x8 Made by: S Crittenden Received: 11/08/02 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3025 Species: Caenorhabditis elegans Genotype: gld-1(q485) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Segregates WT GFP+ heterozygotes, non-GFP sterile gld-1 homozygotes, very rare GFP+ homozygous hT2, and dead eggs. Maintain by picking wild-type GFP+. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Reference: Jeong J, et al. PLoS Genet. 2011 Mar;7(3):e1001348. Mutagen: Outcrossed: x Made by: S. Crittenden Received: 05/22/12 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3051 Species: Caenorhabditis elegans Genotype: qEx454. Description: qEx454 [gon-1::GFP + rol-6(su1006)]. Pick Rollers. GFP expressed in muscle, pharynx and DTC. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: M. Chesney Received: 07/25/08 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3072 Species: Caenorhabditis elegans Genotype: gon-16(q568) IV/nT1 [qIs51] (IV;V). Description: Heterozygotes are WT and GFP+. q568(ts) homozygotes are sterile and can exhibit a white-patch phenotype. The severity of the white-patch increases with increasing temperature. nT1[qIs51] is probably homozygous lethal. qIs51 is an insertion of ccEx9747 with markers: myo-2::GFP expressed in the pharynx throughout development, pes-10::GFP expressed in the embryo, and a gut promoter F22B7.9::GFP expressed in the intestine. Crosses with this strain generate very few glowing hermaphrodite cross progeny and many glowing male cross progeny. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x6 Made by: K. Siegfried Received: 04/27/04 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3073 Species: Caenorhabditis elegans Genotype: gon-15(q574) IV/nT1 [qIs51] (IV;V). Description: Heterozygotes are WT and GFP+. q574 homozygotes are sterile at all temperatures and exhibit a white-patch phenotype at 25C. nT1[qIs51] is probably homozygous lethal. qIs51 is an insertion of ccEx9747 with markers: myo-2::GFP expressed in the pharynx throughout development, pes-10::GFP expressed in the embryo, and a gut promoter F22B7.9::GFP expressed in the intestine. Crosses with this strain generate very few glowing hermaphrodite cross progeny and many glowing male cross progeny. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x8 Made by: K. Siegfried Received: 04/27/04 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3083 Species: Caenorhabditis elegans Genotype: ehn-3(q689) IV. Description: unc-24 was recombined onto and off of ehn-3(q689) chromosome. Reference: Mathies et al. (2003) Development 130(13):2881-92. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x15 Made by: Laura Mathies Received: 04/08/10 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: JK3087 Species: Caenorhabditis elegans Genotype: ehn-3(q689) unc-5(e53) IV. Description: Unc. About 20% have single gonad arm. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x10 Made by: L. Mathies Received: 01/16/04 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3091 Species: Caenorhabditis elegans Genotype: ehn-1(q690) rol-6(e187) II. Description: Rollers. About 15% have a one-armed gonad or are WP. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x10 Made by: L. Mathies Received: 01/16/04 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3101 Species: Caenorhabditis elegans Genotype: fbf-2(q738) II. Description: Grows well as a homozygote, possibly small percent Fog. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x10 Made by: L. Lamont Received: 03/25/05 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3107 Species: Caenorhabditis elegans Genotype: fbf-1(ok91) fbf-2(q704)/mIn1 [dpy-10(e128) mIs14] II. Description: Heterozygotes are non-Dpy with a green pharynx and a WT germ line. fbf-1 fbf-2 homozygotes are non-Dpy, GFP-, and have small germ lines containing only sperm. mIn1[mIs14 dpy-10(e128)] homozygotes are Dpy, GFP+ and have a WT germ line. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x3 Made by: S. Crittenden Received: 11/08/02 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3140 Species: Caenorhabditis elegans Genotype: gon-1(e2551) IV/nT1 [qIs51] (IV;V). Description: Heterozygotes are WT and GFP+. Segegrate Gon worms which are GFP-. nT1[qIs51] homozygotes are inviable. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: D. Hesselson Received: 09/16/04 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3172 Species: Caenorhabditis elegans Genotype: mig-5&cct-1(ok280)/mIn1 [mIs14 dpy-10(e128)] II. Description: T05C12.6, T05C12.7. Heterozygotes are WT and GFP+ in the pharynx. mIn1[mIs14 dpy-10(e128)] homozygotes are Dpy and GFP+ in the pharynx. Homozygous mig-5&cct-1(ok280) worms arrest at about L2/L3. Strong loss of function for cct-1 and weak for mig-5. mig-5 is a dishelved homologue. External left primer: CGGCCTAAACGTTGATTGTT. External right primer: CAGAGTGAGTCGTGAACCGA. Internal left primer: TAATCCTGAATCCGGACGAG. Internal right primer: TACGAGATTTCGGTCCCTTG. Internal WT amplicon: 3284 bp. Deletion size: 987 bp. Deletion left flank: GCTGCAGTCTGATGTGCATGGCGGCTCCAT. Deletion right flank: TTGGTGTTGTCTATGCTTCAAGAAAATTGA. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: UV/TMP Outcrossed: x5 Made by: OMRF & K Siegfried Received: 01/07/04 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3182 Species: Caenorhabditis elegans Genotype: gld-3(q730) nos-3(q650)/mIn1 [mIs14 dpy-10(e128)] II. Description: Heterozygotes are WT and GFP+. mIn1 homozygotes are Dpy and GFP+. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: C. Eckmann Received: 06/05/08 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3203 Species: Caenorhabditis elegans Genotype: puf-7(ok397) IV. Description: B0273.2. External left primer: AAGCATACAGGCGCAGAGAT. External right primer: TACCGTATTGTGGTGCGAAA. Internal left primer: AACGGTTGCTCATCTGACTT. Internal right primer: AAAATTGCGTCGATTTTTGG. Internal WT amplicon: 2701 bp. Deletion size: 1751 bp. Deletion left flank: TTAAAACTTCTAATTTAAATAAAAAATATA. Deletion right flank: TATGTCGTTCAGCAAATGATTTCGATTTGC. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: UV/TMP Outcrossed: x0 Made by: Received: 03/25/05 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3221 Species: Caenorhabditis elegans Genotype: sys-1(q736) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT, but frequently sterile when balanced by hT2[qIs48]. q736 is homozygous embryonic lethal. (Approx. 5% of hets are Sterile when balanced by WT.) hT2[qIs48] homozygotes are inviable. q736 is a deletion of sys-1 deleting from nucleotide 1803 to nucleotide 3992 of the genomic sequence (a of start atg is position 1). Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x7 Made by: T. Kidd Received: 07/01/05 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK323 Species: Caenorhabditis elegans Genotype: qDf3/szT1 [lon-2(e678)] I; +/szT1 X. Description: Heterozygotes are WT and grow slowly. Segregates Lon males. Do not grow at 25C. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Gamma Rays Outcrossed: x Made by: M.K. Barton Received: 10/13/94 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3231 Species: Caenorhabditis elegans Genotype: puf-8(q725) II. Description: Low penetrance (<10%) Mog. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x7 Made by: J. Bachorik Received: 01/13/06 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3263 Species: Caenorhabditis elegans Genotype: gon-14(q686) V. Description: Temperature sensitive. At 25C, q686 have 0-2 gonad arms and are sterile. Some are Pvl. The strongest phenotype is a spf-1-like gonad. At 15C, q686 is 100% fertile. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x7 Made by: M. Chesney Received: 12/14/05 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3276 Species: Caenorhabditis elegans Genotype: hnd-1(q740) X. Description: q740 homozygotes throw about 50% gonadogenesis defective worms (can be gonadless or have a single arm gonad). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x13 Made by: L. Mathies Received: 01/16/04 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3297 Species: Caenorhabditis elegans Genotype: fbl-1(q750) IV/nT1 [qIs51] (IV;V). Description: Heterozygotes are WT and GFP+ and segregate q750 homozygotes which are GFP- sterile adults, and dead eggs. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: TMP Outcrossed: x3 Made by: D. Hesselson Received: 03/25/05 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3345 Species: Caenorhabditis elegans Genotype: gld-3(q741)/mIn1 [mIs14 dpy-10(e128)] II. Description: Heterozygotes are WT with major GFP signal in pharynx. Segregates WT GFP+, Dpy GFP+ (mIn1 homozygotes) and GFP- gld-3 homozygotes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x8 Made by: C. Eckmann Received: 12/03/04 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3361 Species: C. elegans Genotype: qIs76 I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: qIs76 [tra-1::GFP::beta-GAL + rol-6(su1006)]. Rollers express TRA-1::GFP in Z1/Z4, intestine, etc. qIs76 homozygotes are lethal. Heterozygotes are Rol GFP+ (pharynx). Pick Rollers with GFP+ pharynx to maintain. Reference: Chang W., et al. Development. 2004 Mar;131(6):1425-36. Mutagen: Gamma ray Outcrossed: x5x Made by: Laura Mathies Received: 07/31/19 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3363 Species: Caenorhabditis elegans Genotype: qIs69 V. Description: qIs69 [hnd-1::GFP::lacZ + rol-6(su1006)] V. GFP expression in MS, C, and D lineages, then Z1/Z4. Rollers. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Gamma radiation Outcrossed: x5 Made by: Laura Mathies Received: 03/02/10 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3375 Species: Caenorhabditis elegans Genotype: gld-3(q730)/mIn1 [mIs14 dpy-10(e128)] II; him-5(e1490) V. Description: Heterozygotes are WT with major GFP signal in pharynx. Segregates WT GFP+, Dpy GFP+ (mIn1 homozygotes) and GFP- gld-3 homozygotes. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x9 Made by: C. Eckmann Received: 12/03/04 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3437 Species: Caenorhabditis elegans Genotype: him-5(e1490) V; qIs74. Description: qIs74 [sys-1p::GFP::pop-1 + unc-119(+)]. Probably on LG X. May have unc-119 in background. Throws 30% males. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: L. Mathies Received: 04/08/04 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3439 Species: Caenorhabditis elegans Genotype: ehn-3(q766) IV. Description: Occasional one-armed gonads. Reference: Mathies et al. (2004) Development 131(17):4333-43. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x7 Made by: Laura Mathies Received: 04/08/10 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: JK3447 Species: Caenorhabditis elegans Genotype: fog-1(q250)/sep-1(e2406) I; fbf-1(ok91) fbf-2(q704)/mIn1 [mIs14 dpy-10(e128)] II. Description: To maintain, check individual fertile worms for both Sep and Fog offspring. sep-1 homozygotes are sterile at 20C and 25C (Sterile and Unc at 25C), and mostly normal at 15C. fog-1; fbf-1 fbf-2 homozygotes have small germ lines and are often Muv. Best to maintain at 25C for scoring. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: B. Thompson Received: 01/14/09 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3476 Species: Caenorhabditis elegans Genotype: ceh-22(q632) sma-1(e30) V/nT1 [qIs51] (IV;V). Description: Heterozygotes are WT and GFP+. Segregates Sma, GFP- worms which have partially penetrant gonadogenesis defects. nT1[qIs51] is probably homozygous lethal. qIs51 is an insertion of ccEx9747 with markers: myo-2::GFP expressed in the pharynx throughout development, pes-10::GFP expressed in the embryo, and a gut promoter F22B7.9::GFP expressed in the intestine. Crosses with this strain generate very few glowing hermaphrodite cross progeny and many glowing male cross progeny. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x Made by: M. Chesney Received: 04/27/04 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3501 Species: Caenorhabditis elegans Genotype: pop-1(q772) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT and GFP+. qIs48 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter driving GFP in the intestine, and is homozygous lethal. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. q772 is embryonic lethal. Mutagen: Outcrossed: x5 Made by: T Kidd Received: 05/19/09 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3528 Species: Caenorhabditis elegans Genotype: qEx500. Description: qEx500 [hs::sys-1 + rol-6(su1006)]. Pick Rollers to maintain. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: T. Kidd Received: 06/17/05 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3695 Species: Caenorhabditis elegans Genotype: qIs87. Description: qIs87 contains [UAS::fkh-6::GFP + rol-6(su1006)]. Rollers. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Chris Tilmann Received: 09/10/09 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3743 Species: Caenorhabditis elegans Genotype: fog-1(q785) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT and GFP+. qIs48 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter driving GFP in the intestine, and is homozygous lethal. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x8 Made by: B. Thompson Received: 01/11/12 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3744 Species: Caenorhabditis elegans Genotype: qIs89. Description: qIs89 [gon-14::gon-14::VENUS + S. cerevisiae DNA]. gon-14::VENUS is broadly expressed in cell nuclei starting at the 50-100 cell stage of embryogenesis and throughout larval development. Partially concentrated in nuclear speckles. qIs89 rescues the 25C Gon and Ste defects of q686. Low penetrance of sterility and lethality in the strain. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: M. Chesney Received: 06/05/08 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3776 Species: Caenorhabditis elegans Genotype: qIs90. Description: qIs90 [ceh-22b::VENUS (pJK1082) + influenza viral DNA]. qIs90 is an integrated complex array; integration site not mapped. ceh-22b::VENUS can be easily identified by its expression in the pharynx. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x5 Made by: N. Lam Received: 10/03/07 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3782 Species: C. elegans Genotype: qIs56 him-5(e1490) V; qEx557. Description: qIs56 [lag-2p::GFP + unc-119(+)] V. qEx557 [hsp-16p::ceh-22b + ttx-3::DsRed]. Him. Pick DsRed+ animals to maintain qEx557 array. Heat-shock can be used to drive the ectopic expression of CEH-22B (derived from Fire Lab vector pPD49.78). LAG-2::GFP is expressed the embryo, nerve cord, Z1/Z4, and DTCs. Reference: Lam N. et al. Curr Biol. 2006 Feb 7;16(3):287-95. doi: 10.1016/j.cub.2005.12.015. PMID: 16461282 Mutagen: Injection Outcrossed: x0 Made by: Ngan Lam Received: 12/07/21 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3791 Species: Caenorhabditis elegans Genotype: sys-1(q544) I; qIs95 III. Description: qIs95 [(sys-1p::Venus::sys-1 + pttx-3::DsRed]. The DsRed marker is very dim and might be difficult to see it under the dissecting scope. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: B. Phillips Received: 08/27/07 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3826 Species: Caenorhabditis elegans Genotype: mut-16(mg461) I; larp-1(q783) III. Description: Slow growing and throw about 10% dead embryos. q783 is a deletion of the first 4 exons on the larp-1 gene. NOTE: this strain is carrying mut-16(mg461) in the background; It is unknown if mg461 is homozygous in this strain. See JK4545 for a replacement larp-1(q783) strain. mut-16 can be detected using primer1 CCCGCCGATACAGAAACTAA, primer 2 AATATTCGATCGGCAAGCAG for genotyping. The wild-type locus will yield a 824bp PCR product, whereas mg461 will yield a 373bp product. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x7 Made by: K Nykamp Received: 03/15/25 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3934 Species: Caenorhabditis elegans Genotype: gld-1(q93) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I; III). Description: Heterozygotes are WT, GFP+ in the pharynx and segregate WT (GFP+ in the pharynx), dead eggs (hT2 homozygotes) and non-GFP gld-1(q93) homozygotes with masculinized germlines. Some heterozygotes will also have a masculinized germline. Maintain by picking GFP worms and checking for correct segregation, since the hT2 balancer is lost at low frequencies. gld-1(q93) was isolated as a dominant suppressor of fem-1(hc17). Mutagen: Outcrossed: x Made by: Liana Lamont Received: 07/01/16 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3961 Species: C. elegans Genotype: puf-8(q725)/mIn1[mIs14 dpy-10(e128)] II; lip-1(zh15) IV. Description: Pick wild-type GFP+ to maintain. q725 heterozygotes are wild-type with pharyngeal GFP signal, and segregate wild-type GFP+ q725 heterozygotes, Dpy bright GFP+ (mIn1 homozygotes), and non-GFP puf-8(q725) II; lip-1(zh15) IV double homozygotes. Homozygous puf-8(q725) II; lip-1(zh15) IV double mutants are ~100% Mog at 20C and ~100% Tumerous at 25C. Reference: Morgan CT, et al. Nat Chem Biol. 2010 Feb;6(2):102-4. Mutagen: Outcrossed: x0 Made by: Myon-Hee Lee Received: 05/09/19 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK3965 Species: Caenorhabditis elegans Genotype: fbf-1(ok91) fbf-2(q704)/mIn1 [mIs14 dpy-10(e128)] II; fem-3(e1996)/qIs24 IV. Description: qIs24 [lag-2p::GFP]. Maintain by picking individual animals with green DTCs and green pharynx and scoring offspring for Fems and Fbfs. fem-3 is not well balanced by qIs24. fbf-1 fbf-2 homozygotes are germline proliferation defective and make only sperm. fem-3 homozygotes make only oocytes. fbf-1 fbf-3; fem-3 proliferates more than fbf-1 fbf-2 and makes only oocytes; also Muv. qIs24 contains lag-2::GFP; Distal Tip Cells are green; homozygotes are Rollers and heterozygotes Roll weakly. mIn1 is Dpy and GFP+ in the pharynx. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: S. Crittenden Received: 01/14/09 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4087 Species: Caenorhabditis elegans Genotype: rnp-8(q784) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT and GFP+. qIs48 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter driving GFP in the intestine, and is homozygous lethal. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. rnp-8 homozyotes are GFP- and look like WT. Slow L4 to Adult transition. Mutagen: Outcrossed: x7 Made by: KW Kim Received: 05/19/09 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4099 Species: Caenorhabditis elegans Genotype: hlh-2(tm1768) I. Description: Temperature sensitive sterile. Partially sterile at 20 C; completely sterile at 25 C. Maintain at <20 C. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x7 Made by: M Chesney Received: 01/07/10 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4143 Species: Caenorhabditis elegans Genotype: qIs57 II; rde-1(ne219) V; qIs140. Description: qIs57 [lag-2p::GFP] II. qIs140 [lag-2p::rde-1 + rol-6(su1006)]. Rollers. qIs140 rescues rde-1 in lag-2-expressing cells (as tested by GFP RNAi). qIs57 GFP expression in DTCs. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x2 Made by: D. Byrd Received: 03/26/13 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4174 Species: C. elegans Genotype: K10D2.2(q792) III. Description: Superficially wild-type. K10D2.2 is a gld-2 paralog. Mutagen: EMS Outcrossed: x6 Made by: Peggy Kroll-Conner Received: 09/29/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4177 Species: C. elegans Genotype: pup-3(q793) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP q793 homozygotes (viable and somewhat fertile). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Mutagen: EMS Outcrossed: x6 Made by: Peggy Kroll-Conner Received: 09/29/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4220 Species: C. elegans Genotype: puf-6(q759) II. Description: Superficially wild-type. Mutagen: UV-TMP Outcrossed: x6 Made by: Peggy Kroll-Conner Received: 09/29/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4278 Species: C. elegans Genotype: puf-12(q810) II. Description: Superficially wild-type. Mutagen: EMS Outcrossed: x4 Made by: Aaron Kerschner Received: 09/29/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4299 Species: C. elegans Genotype: gld-2(q497) gld-1(q361) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Pick GFP+ heterozygotes to maintain. Segregates fertile GFP+ heterozygotes, non-GFP homozygous mutants (Gld; form germline tumors), very rare GFP+ homozygous hT2, and dead eggs. gld-1(q361) is a missense allele but phenotypically null, which allows the detection of gld-1 mRNA and protein by single molecule FISH or antibody staining. Reference: Hansen et al (2004) Dev. Biol. 2004;268(2):342-57. Shin et al. (2017) PLoS Genet. 2017;13(12):e1007121. Mutagen: N/A Outcrossed: x0 Made by: Olivier Cinquin Received: 01/24/18 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4307 Species: Caenorhabditis elegans Genotype: rnp-8(tm2435) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT and GFP+. qIs48 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter driving GFP in the intestine, and is homozygous lethal. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. PCR using 3 primers: primer A: TCA GCC GAC AAT CTC CGA; primer B: GTA CTG ATT GTA TCC ACC GGC; primer C: GCT CAT AGA AAA GCG ATG G. WT band = 0.5 kb; heterozygote bands = 0.8 and 0.5 kb (double bands), tm2435 bands = 0.8 kb. Mutagen: Outcrossed: x13 Made by: KW Kim Received: 05/19/09 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4472 Species: Caenorhabditis elegans Genotype: qIs154 V. Description: qIs154 [lag-2p::MYR::tdTomato + ttx-3p::GFP] V. Myristylated tdTomato localizes to plasma membrane of DTC and other lag-2-expressing cells. Reference: Byrd DT, et al. PLoS One. 2014 Feb 19;9(2):e88372. Mutagen: Outcrossed: x4 Made by: Karla Knobel Received: 04/22/14 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4475 Species: Caenorhabditis elegans Genotype: qIs153 V. Description: qIs153 [lag-2p::MYR::GFP + ttx-3p::DsRed] V. Myristylated GFP localizes to plasma membrane of DTC and other lag-2-expressing cells. Reference: Byrd DT, et al. PLoS One. 2014 Feb 19;9(2):e88372. Mutagen: Outcrossed: x2 Made by: Karla Knobel Received: 04/22/14 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4545 Species: Caenorhabditis elegans Genotype: larp-1(q783) III. Description: Derived by outcrossing JK3826 to remove mut-16 deletion. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: N Nykamp Received: 12/04/10 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4563 Species: Caenorhabditis elegans Genotype: gld-1(q126) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Segregates WT GFP+ heterozygotes, non-GFP sterile gld-1 homozygotes, very rare GFP+ homozygous hT2, and dead eggs. Maintain by picking wild-type GFP+. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: P. Kroll-Connor Received: 12/16/09 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4619 Species: C. elegans Genotype: tra-1(q106) III/eT1[qIs60](III;V). Description: qIs60 [pes-10::GFP + gut specific promoter::GFP + myo-2::GFP]. Heterozygotes are wild-type GFP+ and segregate wild-type GFP+ heterozygotes, non-GFP males, and dead eggs (eT1 homozygotes). Pick wild-type GFP+ and check for proper segregation of progeny to maintain. Reference: Schedl T, et al. Genetics. 1989 Dec;123(4):755-69. doi: 10.1093/genetics/123.4.755. PMID: 2612895. Mutagen: EMS Outcrossed: x0 Made by: Peggy Kroll-Conner Received: 09/29/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4626 Species: Caenorhabditis elegans Genotype: cku-80(ok861) unc-119(ed3) III; qIs170. Description: qIs170 [gld-1p::gld-1::GFP::FLAG + unc-119(+)]. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Reference: Jeong J, Verheyden JM, Kimble J. PLoS Genet. 2011 Mar;7(3):e1001348. Mutagen: Bombardment Outcrossed: x Made by: J. Jeong Received: 08/17/12 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4774 Species: Caenorhabditis elegans Genotype: lst-1(ok814) sygl-1(tm5040) I/hT2[bli-4(e937) let-?(q782) qIs48] (I;III). Description: Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP sterile homozygotes. Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Kershner AM, et al. Proc Natl Acad Sci U S A. 2014 Mar 11;111(10):3739-44. Mutagen: Outcrossed: x0 Made by: Heaji Shin Received: 08/25/14 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4832 Species: C. elegans Genotype: gld-1(q485) gld-2(q497) lst-1(ok814) sygl-1(tm5040) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Pick GFP+ heterozygotes to maintain. Segregates fertile GFP+ heterozygotes, non-GFP homozygous mutants (Gld; form germline tumors), very rare GFP+ homozygous hT2, and dead eggs. Reference: Kershner et al. (2014) PNAS 111: 3739-3744. Mutagen: Outcrossed: x0 Made by: Heaji Shin Received: 01/24/18 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4842 Species: Caenorhabditis elegans Genotype: qSi29 II; unc-119(ed3) III. Description: qSi29 [sygl-1p(LBSmut)::H2B::GFP::sygl-1 3'end + unc-119(+)]. Superficially wild-type. Expression of H2B::GFP in the loop region of the germline. qSi29 contains 2kb upstream of the sygl-1 start with the four LAG-1 binding sites (LBS) mutated (RTGRGAA->RacRGAA) driving H2B::GFP under the control of the sygl-1 3' intergenic region. Reference: Kerschner AM, et al. Proc Natl Acad Sci U S A. 2014 Mar 11;111(10):3739-44. Mutagen: Outcrossed: x Made by: Aaron Kershner Received: 01/09/15 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4862 Species: Caenorhabditis elegans Genotype: glp-1(q46) III/hT2[bli-4(e937) let-?(q782) qIs48] (I;III). Description: Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP sterile homozygotes. Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Austin J & Kimble J. Cell. 1987 Nov 20;51(4):589-99. Mutagen: Outcrossed: x0 Made by: Erika Sorensen Received: 08/25/14 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4864 Species: C. elegans Genotype: qIs147 IV. Description: qIs147 [sur-5::GFP] IV. Reference: Lynch TR, et al. Development. 2022 Apr 1;149(7):dev200332. PMID: 35394007. The sur-5::GFP reporter is described in Yochem J, et al. Genetics. 1998 Jul;149(3):1323-34. PMID: 9649523. Mutagen: UV/TMP Outcrossed: x Made by: Doug Porter and Dan Noble Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4871 Species: Caenorhabditis elegans Genotype: fog-3(q520) I; qSi41 II. Description: qSi41 [fog-3::3xFLAG + unc-119(+)] inserted into ttTi5605 on LG II. Can be maintained at 20C. qSi41 rescues fog-3 null phenotype. References: Noble DC, et al. Genetics. 2016 Jan;202(1):221-34. Mutagen: MosSCI Outcrossed: x Made by: Dan Noble Received: 02/11/16 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4930 Species: C. elegans Genotype: lag-1(q426) IV/nT1[qIs51](IV; V). Description: Homozygous lethal deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP q426 homozygotes (variable phenotypes including sterile, L1 lethal, and small sterile adults with rectal protrusion and occasional bent pharynx). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Kadyk LC & Kimble J. Development. 1998 May;125(10):1803-13. doi: 10.1242/dev.125.10.1803. PMID: 9550713. Mutagen: EMS Outcrossed: x0 Made by: Peggy Kroll-Conner Received: 09/29/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4942 Species: C. elegans Genotype: sygl-1(tm5040) I; qSi49 II; unc-119(ed3) III. Description: qSi49 [sygl-1p::3xFLAG::sygl-1::sygl-1 3’UTR + unc-119(+)]. Superficially wild-type. Unknown whether or not unc-119(ed3) is still present in the background. Reference: Shin H, et al. PLoS Genet. 2017 Dec 12;13(12):e1007121. Mutagen: Outcrossed: x0 Made by: Heaji Shin Received: 01/22/20 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK4996 Species: C. elegans Genotype: lst-1(ok814) I; qSi69 II; unc-119(ed3) III Description: qSi69 [lst-1p::lst-1::3xFLAG::lst-1 3’UTR + unc-119(+)]. Superficially wild-type. Reference: Shin H, et al. PLoS Genet. 2017 Dec 12;13(12):e1007121. Mutagen: Outcrossed: x0 Made by: Aaron Kershner Received: 01/22/20 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5008 Species: C. elegans Genotype: qSi44 II; glp-1(q46) III. Description: qSi44 [glp-1::6xMyc6xHis + Cbr-unc-119(+)] II. Homozygous viable. Variable body length. May still carry unc-119(ed3) in the background. Reference: Sorensen EB, et al. A toolkit of tagged glp-1 alleles reveals strong glp-1 expression in the germline, embryo, and spermatheca. microPublication Biology, 2020(06). http://doi.org/10.17912/micropub.biology.000271 Mutagen: NA Outcrossed: x3 Made by: Erika Sorensen Received: 08/12/20 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5018 Species: Caenorhabditis elegans Genotype: qSi26 II; unc-119(ed3) III; teIs1 IV. Description: qSi26 [sygl-1p::H2B::GFP::sygl-1 3' end + unc-119(+)]. GFP expression in distal germ cell nuclei. teIs1 [oma-1::GFP + unc-119(+)]. oma-1::GFP expression in oocyte cytoplasm. teIs1 rescues GFP expression in silenced germline trangenes more effectively at 25C. Reference: Kerschner AM, et al. Proc Natl Acad Sci U S A. 2014 Mar 11;111(10):3739-44. Mutagen: Outcrossed: x0 Made by: Sarah Crittenden Received: 01/09/15 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5020 Species: C. elegans Genotype: glp-1(q172) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP q172 homozygotes (sterile). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Crittenden SL, et al. Development. 1994 Oct;120(10):2901-11. doi: 10.1242/dev.120.10.2901. PMID: 7607080. Mutagen: EMS Outcrossed: x0 Made by: Peggy Kroll-Conner Received: 09/29/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5028 Species: Caenorhabditis elegans Genotype: qSi77 II; unc-119(ed3) III. Description: qSi77 [mex-5p::eGFP::3xFLAG::tbb-1 3'utr::gpd-2 SL2 splice site::mCherry::3xMyc::pgl-1 RGG repeat::tbb-1 3'utr and intergenic region + unc-119(+)] inserted into ttTi5605 on LG II. References: Noble DC, et al. Genetics. 2016 Jan;202(1):221-34. Mutagen: MosSCI Outcrossed: x3 Made by: Scott Aoki Received: 02/11/16 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5072 Species: Caenorhabditis elegans Genotype: qSi29 II; unc-119(ed3) III; teIs1 IV. Description: qSi29 [sygl-1p(LBSmut)::H2B::GFP::sygl-1 3'end + unc-119(+)]. teIs1 [oma-1::GFP + unc-119(+)]. Superficially wild-type. Expression of H2B::GFP in the loop region of the germline. qSi29 contains 2kb upstream of the sygl-1 start with the four LAG-1 binding sites (LBS) mutated (RTGRGAA->RacRGAA) driving H2B::GFP under the control of the sygl-1 3' intergenic region. oma-1::GFP expression in oocyte cytoplasm. teIs1 rescues GFP expression in silenced germline trangenes more effectively at 25C. Reference: Kerschner AM, et al. Proc Natl Acad Sci U S A. 2014 Mar 11;111(10):3739-44. Mutagen: Outcrossed: x0 Made by: Aaron Kershner Received: 01/09/15 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK509 Species: Caenorhabditis elegans Genotype: glp-1(q231) III. Description: Temperature sensitive. Sterile at 25C. Fertile at 15C. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 07/11/96 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5187 Species: Caenorhabditis elegans Genotype: fog-1(q785) I; qSi140 IV. Description: qSi140 [3xMyc::fog-1 + unc-119(+)] inserted into cxTi10816 on LG IV. Can be maintained at 20C. qSi140 rescues fog-1 null phenotype. References: Noble DC, et al. Genetics. 2016 Jan;202(1):221-34. Mutagen: MosSCI Outcrossed: x Made by: Dan Noble Received: 02/11/16 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5200 Species: Caenorhabditis elegans Genotype: fog-1(q785) fog-3(q520) I; qSi41 II; qSi140 IV. Description: qSi41 [fog-3::3xFLAG + unc-119(+)] inserted into ttTi5605 on LG II. qSi140 [3xMyc::fog-1 + unc-119(+)] inserted into cxTi10816 on LG IV. Can be maintained at 20C. qSi41 rescues fog-3 null phenotype. qSi140 rescues fog-1 null phenotype. References: Noble DC, et al. Genetics. 2016 Jan;202(1):221-34. Mutagen: MosSCI Outcrossed: x0 Made by: Dan Noble Received: 02/11/16 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5226 Species: C. elegans Genotype: glp-1(q46) III; qSi156 IV. Description: qSi156 [glp-1::Halotag + Cbr-unc-119(+)] IV. Mos insertion of Halo tagged GLP-1 in glp-1(null) background. qSi156 mostly rescues glp-1(q46) sterility; partially penetrant embryonic lethality, early larval lethality and dumpiness. Reference: Sorensen EB, et al. A toolkit of tagged glp-1 alleles reveals strong glp-1 expression in the germline, embryo, and spermatheca. microPublication Biology, 2020(06). http://doi.org/10.17912/micropub.biology.000271 Mutagen: Mos Outcrossed: x0x Made by: Hannah Seidel Received: 08/12/20 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5250 Species: C. elegans Genotype: pole-1(q831)/hIn1[unc-54(h1040)] I. Description: Heterozygotes are fertile WT (non-Unc) and segregate fertile WT (q831/hIn1), fertile Unc (hIn1/hIn1), and sterile non-Uncs (q831/q831). Pick fertile WT and check for correct segregation of progeny to maintain. Reference: Robinson-Thiewes S, et al. G3 (Bethesda). 2022 Mar 4;12(3):jkab439. doi: 10.1093/g3journal/jkab439. PMID: 35100350. Mutagen: EMS Outcrossed: x8 Made by: Aaron Kershner Received: 09/29/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5305 Species: C. elegans Genotype: lst-1(q827) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP q827 homozygotes (viable and somewhat fertile). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Robinson-Thiewes S, et al. G3 (Bethesda). 2022 Mar 4;12(3):jkab439. doi: 10.1093/g3journal/jkab439. PMID: 35100350. Mutagen: EMS Outcrossed: x8 Made by: Heaji Shin and Kim Haupt Received: 10/23/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5455 Species: C. elegans Genotype: q833 V/nT1[qIs51](IV; V). Description: hets are green pharynx WT and segregate green pharynx WT, fertile non-green pharynx and dead eggs (nT1 homozygotes). q833 is a 472 bp deletion into the intergenic region between mir-61/ mir-250 and F55A11.4. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Erika Sorenson Received: 09/29/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5500 Species: C. elegans Genotype: sygl-1(q828) I; qSi150 II. Description: qSi150 [sygl-1p::3X flag::sygl-1::tbb-2 3' UTR + Cbr-unc-119(+)] II. Phenotypically gravid, grows as a homozygote. Increased SYGL-1 protein expression, expanded progenitor zone, expanded GSC pool. Reference: Shin H. et al. PLoS Genet. 2017 Dec 12;13(12):e1007121. Mutagen: Outcrossed: x0 Made by: Heaji Shin Received: 12/11/18 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK551 Species: Caenorhabditis elegans Genotype: unc-5(e53) fem-3(q22) IV. Description: Temperature sensitive. At 25C, XX germline makes only sperm; at 15C, germline makes oocytes and excess sperm. Unc. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 10/01/86 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5535 Species: C. elegans Genotype: glp-1(q46) III; qSi246 IV. Description: qSi246 [glp-1::sfGFP + Cbr-unc-119(+)] IV. Mos insertion of sfGFP tagged GLP-1 in glp-1(0) background. qSi246 rescues glp-1(q46) sterile phenotype. Animals are fertile, superficially wild-type with GFP+ distal germ-lines. Reference: Sorensen EB, et al. A toolkit of tagged glp-1 alleles reveals strong glp-1 expression in the germline, embryo, and spermatheca. microPublication Biology, 2020(06). http://doi.org/10.17912/micropub.biology.000271 Mutagen: Mos Outcrossed: x0 Made by: Erika Sorensen Received: 08/12/20 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK554 Species: Caenorhabditis elegans Genotype: dpy-17(e164) glp-1(q224) III; unc-1(e1598) X. Description: Raise at 15C. Dpy Unc. unc-102(e1598) changed to unc-1(e1598). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 04/03/96 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5556 Species: C. elegans Genotype: F17E5.2(q864) X. Description: 350 bp deletion in F17E5.2 Mutagen: Crispr/Cas9 Outcrossed: x3 Made by: Erika Sorensen Received: 09/29/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5596 Species: C. elegans Genotype: lst-1(q867) I. Description: CRIPSR-engineered mutation of the lst-1M71 longform start codon (ATG to AT, deletion of G). Reference: Haupt KA, et al. Development. 2019 Oct 17;146(20):dev181644. doi: 10.1242/dev.181644. PMID: 31515205 Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Alex Murphy Received: 09/29/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK560 Species: Caenorhabditis elegans Genotype: fog-1(q253) I. Description: Temperature sensitive - raise at 15 or 20C. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x>1 Made by: A. Petcherski Received: 03/27/00 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5657 Species: Caenorhabditis elegans Genotype: tra-1(q183) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I; III). Description: Maintain at 15C. Heterozygotes are WT, GFP+ in the pharynx and segregate WT (GFP+ in the pharynx), dead eggs (hT2 homozygotes) and non-GFP tra-1(q183) homozygous females. Raise strain at 15C; at 20C many heterozygotes are also feminized. tra-1(q183) is temperature-sensitive for its XX, but not its XO mutant phenotype. At 25C, both q183/q183 and q183/+ are female. At 15C, q183/q183 is still always female, but q183/+ can be hermaphroditic. Maintain by picking fertile GFP worms and checking for correct segregation, since the hT2 balancer is lost at low frequencies. Mutagen: Outcrossed: x Made by: Peggy Kroll-Conner Received: 07/01/16 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5687 Species: C. elegans Genotype: pgl-1(q894[pgl-1::SNAP]) IV. Description: SNAP tag inserted into endogenous pgl-1 locus between G713 and G714. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Scott Aoki Received: 10/23/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5731 Species: C. elegans Genotype: cya-1(q903[cya-1::3xMyc]) III. Description: Internal 3xMyc tag inserted into endogenous cya-1 locus. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Hannah Seidel Received: 09/29/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5736 Species: C. elegans Genotype: cyb-2.1(q908[3xMyc::cyb-2.1]) IV. Description: 3xMyc tag inserted at N-terminus of endogenous cyb-2.1 locus. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Hannah Seidel Received: 09/29/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5739 Species: C. elegans Genotype: cyb-2.2(q911[3xMyc::cyb-2.2]) I. Description: 3xMyc tag inserted at N-terminus of endogenous cyb-2.2 locus. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Hannah Seidel Received: 09/29/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK574 Species: Caenorhabditis elegans Genotype: fog-2(q71) V. Description: Male-female strain. Maintain by mating. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 09/29/97 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5742 Species: C. elegans Genotype: cyb-3(q914[cyb-3::3xMyc]) V. Description: Internal 3xMyc tag inserted into endogenous cyb-3 locus. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Hannah Seidel Received: 09/29/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5745 Species: C. elegans Genotype: cyd-1(q917[cyd-1::3xMyc]) II. Description: Internal 3xMyc tag inserted into endogenous cyd-1 locus. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Hannah Seidel Received: 09/29/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5748 Species: C. elegans Genotype: cye-1(q920[3xMyc::cye-1]) I. Description: 3xMyc tag inserted at N-terminus of endogenous cye-1 locus. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Hannah Seidel Received: 09/29/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5751 Species: C. elegans Genotype: cdk-1(q923[3xMyc::cdk-1]) III. Description: 3xMyc tag inserted at N-terminus of endogenous cdk-1 locus. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Hannah Seidel Received: 09/29/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5758 Species: C. elegans Genotype: lst-1(q895[lst-1(long)::3xFLAG]) I. Description: 3xFLAG tag inserted at the C terminus of the endogenous lst-1 locus, after V398 of the long isoform. Reference: Haupt KA, et al. Development. 2019 Oct 17;146(20):dev181644. doi: 10.1242/dev.181644. PMID: 31515205 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Jon Doenier Received: 09/29/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5760 Species: C. elegans Genotype: lst-1(ok814) sygl-1(q828) gld-2(q497) gld-1(q361) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Pick GFP+ heterozygotes to maintain. Segregates fertile GFP+ heterozygotes, non-GFP homozygous mutants (Gld; form germline tumors), very rare GFP+ homozygous hT2, and dead eggs. Reference: Shin et al. (2017) PLoS Genet. 2017;13(12):e1007121. Mutagen: N/A Outcrossed: x0 Made by: Olivier Cinquin Received: 01/24/18 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5796 Species: C. elegans Genotype: lst-1(q869) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP q869 homozygotes (viable and somewhat fertile). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. q869 is a deletion of the entire lst-1 coding sequence as well as 139 bp upstream of M71 start codon and 228 bp downstream of the coding sequence. Reference: Haupt KA, et al. Development. 2019 Oct 17;146(20):dev181644. doi: 10.1242/dev.181644. PMID: 31515205 Mutagen: Crispr/Cas9 Outcrossed: x7 Made by: Kim Haupt Received: 09/29/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5800 Species: C. elegans Genotype: qSi364 IV. Description: qSi364 [3xFLAG::fbf-2(R7/R8 loop deletion) + unc-119(+)] IV. Single-copy MosSCI insertion into oxTi177 IV. R7/R8 loop deletion removes a critical protein-to-protein interface. qSi364 is phenotypically wild-type on its own, but Mog when crossed into fbf-1(ok91) fbf-2(q704) background. Reference: Carrick BH, et al. Dev Cell. 2024 Mar 11;59(5):661-675.e7. doi: 10.1016/j.devcel.2024.01.005. PMID: 38290520. Mutagen: MosSCI Outcrossed: x8 Made by: Brian Carrick Received: 05/31/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5810 Species: C. elegans Genotype: fbf-2(q945[3xFLAG::fbf-2]) II. Description: 3xFLAG tag inserted at N-terminus of endogenous fbf-2 locus. Generated in N2 background. Reference: Ferdous AS, et al. 2023 May 1;150(9):dev201705. doi: 10.1242/dev.201705. PMID: 37070766. Mutagen: CRISPR Outcrossed: x2 Made by: Brian Carrick Received: 05/31/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5842 Species: C. elegans Genotype: fbf-2(q932[3xV5::fbf-2]) II. Description: q932 is 3xV5::fbf-2 tagged endogenous locus. Superficially wild-type. Reference: Shin et al. (2017) PLoS Genet. 2017;13(12):e1007121. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Heaji Shin Received: 01/24/18 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5848 Species: C. elegans Genotype: nos-3(q958[nos-3::1xOLLAS]) II. Description: 1xOLLAS tag inserted into endogenous nos-3 locus via CRISPR/Cas9 engineering. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Brian Carrick Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5868 Species: C. elegans Genotype: cpb-1(q967[nos-3::1xOLLAS]) III. Description: 1xOLLAS tag inserted into endogenous nos-3 locus after L141 within a non-conserved region near the N-terminus. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Brian Carrick Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5893 Species: C. elegans Genotype: sygl-1(q983[3xOLLAS::sygl-1]) I. Description: q983 is a 3xOLLAS::sygl-1 tagged endogenous locus. Superficially wild-type. Reference: Shin et al. (2017) PLoS Genet. 2017;13(12):e1007121. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Heaji Shin Received: 01/24/18 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5896 Species: C. elegans Genotype: qSi369 II; unc-119(ed3) III; qSi370 V. Description: qSi369 [sygl-1p::24xMS2 loops::3xflag::sygl-1::sygl1 3'UTR]. qSi370 [mex-5p:: MS2 Coat Protein::linker::sfGFP::tbb-2 3' UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3' UTR]. Superficially wild-type with expression of sfGFP and nuclear mCherry in germline. qSi369 and qSi370 constitute an MS2 system which allows live visualization of sygl-1 nascent transcripts in the C. elegans germline in a glp-1 mutant background. qSi370 can be prone to silencing, especially after severe starvation; silencing of GFP or mCherry expression can occur independently of one another. Maintain by picking animals with bright GFP and mCherry expression. Reference: Lee C, et al. Dev Cell. 2019 Aug 19;50(4):426-435.e4. Mutagen: NA Outcrossed: x0 Made by: ChangHwan Lee Received: 08/30/19 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK590 Species: C. elegans Genotype: glp-1(q35)/eT1 III; him-5(e1490)/eT1 V. Description: Heterozygotes are wild-type and segregate wild-type heterozygotes, glp-1(q35) homozygotes (Muv steriles), eT1 homozygotes (Unc-36), and males. glp-1(q35) has a semi-dominant multi-vulva phenotype as well as the loss-of-function Glp phenotype (sterility and embryonic lethality). The q35 mutation is a nonsense mutation that eliminates 122 C-terminal amino acids including a PEST sequence. The C terminus was thought to contain a negative regulatory domain that inactivates glp-1 in the VPCs; the inappropriate glp-1(q35) activity can substitute for lin-12 vulval fate determination. References: Austin J & Kimble J. Cell. 1987 Nov 20;51(4):589-99. Mango S, et al. Nature. 1991 Aug 29;352(6338):811-5. Mutagen: EMS Outcrossed: x? Made by: Judith Kimble Received: 10/31/19 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5915 Species: C. elegans Genotype: puf-3(q966) IV. Description: Superficially wild-type. Mutagen: Outcrossed: x3 Made by: Kim Haupt Received: 01/22/20 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5929 Species: C. elegans Genotype: lst-1(q1004[lst-1::V5]) I. Description: q1004 is lst-1::V5 tagged endogenous locus. Superficially wild-type. Reference: Shin et al. (2017) PLoS Genet. 2017;13(12):e1007121. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Kimberly Haupt Received: 01/24/18 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5932 Species: C. elegans Genotype: sygl-1(q828) I; qSi369 II; qSi370 V. Description: qSi369 [sygl-1p::24xMS2 loops::3xflag::sygl-1::sygl1 3'UTR]. qSi370 [mex-5p:: MS2 Coat Protein::linker::sfGFP::tbb-2 3' UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3' UTR]. Superfically wild-type with expression of sfGFP and nuclear mCherry in germline. qSi369 and qSi370 constitute an MS2 system which allows live visualization of sygl-1 nascent transcripts in the C. elegans germline in a glp-1 mutant background. Reference: Lee C, et al. Dev Cell. 2019 Aug 19;50(4):426-435.e4. Mutagen: NA Outcrossed: x0 Made by: Heaji Shin Received: 08/30/19 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5933 Species: C. elegans Genotype: glp-1(q1000[glp-1::4xV5]) III. Description: Endogenous glp-1 locus tagged with 4xV5. Tagged GLP-1 rescues: glp-1(q1000) is fertile and progenitor zone looks wild-type. Reference: Sorensen EB, et al. A toolkit of tagged glp-1 alleles reveals strong glp-1 expression in the germline, embryo, and spermatheca. microPublication Biology, 2020(06). http://doi.org/10.17912/micropub.biology.000271 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Sarah Crittenden Received: 08/12/20 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5935 Species: C. elegans Genotype: fbf-1(ok91) fbf-2(q973[3xFLAG::fbf-2]) II. Description: 3xFLAG tag inserted at N-terminus of endogenous fbf-2 locus in fbf-1 null background (parental strain JK3022). Reference: Carrick BH, et al. Dev Cell. 2024 Mar 11;59(5):661-675.e7. doi: 10.1016/j.devcel.2024.01.005. PMID: 38290520. Mutagen: CRISPR Outcrossed: x2 Made by: Brian Carrick Received: 05/31/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5942 Species: C. elegans Genotype: fog-3(q873[fog-3::3xFLAG]) I; qSi375 II. Description: q873[fog-3(1-262)::GGS::3xFLAG::fog-3(263 Phe)] I. qSi375 [mex-5p::eGFP::linker::his-58::3xboxb::tbb-2 3’UTR] II. The tethering assay allows this strain to be used for determining FOG-3 levels in different genetic backgrounds. Similar fertility to N2 wild type. Reference: Aoki S, et al. Cell Rep. 2018 Jun.26; 23(13):3769-3775 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Scott Aoki Received: 03/03/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5943 Species: C. elegans Genotype: qSi369 II; glp-1(q224) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III); qSi370 V. Description: qSi369 [sygl-1p::24xMS2 loops::3xflag::sygl-1::sygl1 3'UTR]. qSi370 [mex-5p:: MS2 Coat Protein::linker::sfGFP::tbb-2 3' UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3' UTR]. Heterozygotes are wild-type with pharyngeal GFP signal, and segregate wild-type GFP+ heterozygotes, non-GFP pharynx q224 homozygotes (Glp sterile at 20-25C) and dead eggs (hT2 homozygotes). All animals are sfGFP + in the germline, with distal and proximal transcription sites in the nucleus. qSi369 and qSi370 constitute an MS2 system which allows live visualization of sygl-1 nascent transcripts in the C. elegans germline in a glp-1 mutant background. Reference: Lee C, et al. Dev Cell. 2019 Aug 19;50(4):426-435.e4. Mutagen: NA Outcrossed: x0 Made by: Heaji Shin Received: 08/30/19 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5964 Species: C. elegans Genotype: lst-1(q1008[lst-1::3xOLLAS]) I. Description: q1008 is lst-1::3xOLLAS tagged endogenous locus. Superficially wild-type. Reference: Shin et al. (2017) PLoS Genet. 2017;13(12):e1007121. Mutagen: CRISPR-Cas9 Outcrossed: x2 Made by: Kimberly Haupt Received: 01/24/18 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5973 Species: C. elegans Genotype: glp-1(q997[glp-1::2xOLLAS]) III. Description: Endogenous glp-1 locus tagged with 2x OLLAS. Tagged GLP-1 rescues: glp-1(q997) is fertile and progenitor zone looks wild-type. Reference: Sorensen EB, et al. A toolkit of tagged glp-1 alleles reveals strong glp-1 expression in the germline, embryo, and spermatheca. microPublication Biology, 2020(06). http://doi.org/10.17912/micropub.biology.000271 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Sarah Crittenden Received: 08/12/20 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5984 Species: C. elegans Genotype: fbf-2(q1011[*q945]) II. Description: q1011 is an engineered Y479A point mutation in the R7/R8 loop of 3xFLAG-tagged FBF-2. Derived by modification of parental strain JK5810 fbf-2(q945[3xFLAG::fbf-2]) II. Reference: Carrick BH, et al. Dev Cell. 2024 Mar 11;59(5):661-675.e7. doi: 10.1016/j.devcel.2024.01.005. PMID: 38290520. Mutagen: CRISPR Outcrossed: x2 Made by: Brian Carrick Received: 05/31/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5986 Species: C. elegans Genotype: fbf-1(ok91) fbf-2(q1023[*q973])/mIn1[mIs14 dpy-10(e128)] II. Description: q1023 is an engineered Y479A point mutation in the R7/R8 loop of 3xFLAG-tagged FBF-2. Derived by modification of parental strain JK5935 fbf-2(q945[3xFLAG::fbf-2]) II. Pick GFP+ t maintain. Homozygous sterile (Mog) mutation balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP ok91 q1023 homozygotes (sterile Mog). Pick WT dim GFP and check for correct segregation of progeny to maintain. Reference: Carrick BH, et al. Dev Cell. 2024 Mar 11;59(5):661-675.e7. doi: 10.1016/j.devcel.2024.01.005. PMID: 38290520. Mutagen: CRISPR Outcrossed: x2 Made by: Brian Carrick Received: 05/31/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK5996 Species: C. elegans Genotype: puf-11(q971) IV. Description: Superficially wild-type. Mutagen: Outcrossed: x6 Made by: Kim Haupt Received: 01/22/20 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6002 Species: C. elegans Genotype: sygl-1(q1015[sygl-1::V5]) I. Description: q1015 is a sygl-1::V5 tagged endogenous locus. Superficially wild-type. Reference: Shin et al. (2017) PLoS Genet. 2017;13(12):e1007121. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Heaji Shin Received: 01/24/18 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6011 Species: Caenorhabditis elegans Genotype: lst-1(q1032[*q1004]) I. Description: q1004[LST-1::3xV5] is the CRISPR-engineered insertion of a 3xV5 tag at the C-terminus of the endogenous lst-1 locus. q1032 is the CRISPR-engineered mutation C260S & C263S in the Zn finger domain of LST-1 (Haupt et al., 2019) in the q1004 background. Reference: Haupt KA, et al. Development. 2019 Oct 17;146(20):dev181644. doi: 10.1242/dev.181644. PMID: 31515205. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Brandon Taylor Received: 09/11/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6022 Species: C. elegans Genotype: pgl-1(q842) IV. Description: pgl-1 (q842) is a (Q342A) missense mutation. This is a homozygous strain; replaced JK5481. Reference: Aoki ST, et al. Proc Natl Acad Sci U S A. 2016 Feb 2;113(5):1279-84. Mutagen: Crispr/Cas92 Outcrossed: x2 Made by: Aaron Kershner Received: 10/05/17 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6045 Species: C. elegans Genotype: glp-1(q1036) III. Description: Maintain at 15C. Germline tumor formation at 25C. A mix of fertile wild-type and proximal tumorous animals at 20C. glp-1(ar202) V5 CRISPR/Cas9 gene editing was used to insert a 3xV5 tag into C-terminus of GLP-1 between amino acids K(1209) and S(1210) using the same reagents as described for tagging wild-type GLP-1 (Sorensen et al., 2020). GLP-1 ar202V5 germlines express GLP-1 in membranes. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Sarah Crittenden Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6059 Species: C. elegans Genotype: glp-1(q1035[*q1000]) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Pick GFP+ to maintain. CRISPR-engineered RAM mutations in endogenous glp-1 locus with C-terminal 4xV5 tag. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP q1035 homozygotes (sterile). Homozygous hT2[bli-4 let-? qIs48] inviable. Maintain by picking GFP+ heterozygotes and checking for correct segregation of progeny to maintain a balanced stock. Derived by modification of parental strain JK5933 glp-1(q1000). Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Sarah Crittenden Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6063 Species: C. elegans Genotype: puf-8(q1048[3xV5::puf-8]) II. Description: 3xV5 tag inserted into endogenous puf-8 locus via CRISPR/Cas9 engineering. Reference: Ferdous AS, et al. Development. 2023 May 1;150(9):dev201705. PMID: 37070766. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Brian Carrick Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6070 Species: C. elegans Genotype: lst-1(q826) I. Description: Slightly smaller germline mitotic region than wild-type. Reference: Shin et al. (2017) PLoS Genet. 2017;13(12):e1007121. Mutagen: EMS Outcrossed: x8 Made by: Aaron Kershner, Kimberly Haupt Received: 01/24/18 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6080 Species: C. elegans Genotype: puf-3(q1058[3xV5::puf-3]) IV. Description: 3x V5 epitope tags inserted into endogenous puf-3 locus. Expresses functional 3x V5-tagged PUF-3. Reference: Haupt KA, Genetics. 2020 Jan;214(1):147-161. PMID: 31740451 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Kimberly Haupt Received: 05/18/21 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6091 Species: C. elegans Genotype: gld-3(q1065[gld-3L::1xV5]) II. Description: 1xV5 tag inserted into endogenous gld-3 locus, specifically tagging GLD-3L isoform, via CRISPR/Cas9 engineering. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Charlotte Kanzler Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6099 Species: C. elegans Genotype: lag-2(q1049[lag-2::3xV5]) V. Description: CRISPR/Cas9 gene editing was used to insert a 3xV5 tag at the C-terminus of the endogenous lag-2 locus immediately upstream of stop codon. Animals are fertile and express LAG-2 V5 in adult DTCs. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Sarah Crittenden Received: 09/11/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6111 Species: C. elegans Genotype: sygl-1(q1054[*q943]) I. Description: C-teminal V5 epitope tag inserted into endogenous sygl-1 locus that has a CRISPR-engineered mutation of predicted Notch-dependent cis-regulatory element LBS D (Yoo et al., 2004). Reference: Lynch TR, et al. Development. 2022 Apr 1;149(7):dev200332. PMID: 35394007. Mutagen: Crispr/Cas9 Outcrossed: x Made by: T. Lynch Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6140 Species: C. elegans Genotype: nos-3(q902) II; qSi380 IV. Description: qSi380 [mex-5p::eGFP::3xOLASS::linker::his-58::MODC pest::3xboxb::tbb-2 3'UTR::SL2 trans-splice site::mCherry::3xV5::linker::his-58::MODC pest::mutant 3xboxb::tbb-2 3'utr::tbb-1 intergenic region] IV. Worms are fertile at 20C. Improved tethering assay for use in the C. elegans germline. GFP reporter mRNA is under control of a germline-expressed mex-5 promoter and has three boxB stem-loops in its 3'UTR. The RNA-binding protein (RBP) is tagged with lamda-N. The nascent transcript driven by mex-5 promoter is resolved by trans-splicing into two mRNAs that encode distinct reporters. The GFP reporter RNA has three functional boxB stem-loops in its 3'UTR; the mCherry reporter 3'UTR has three mutated boxB stem-loops that do not bind lamda-N and therefore provides an internal control. Addition of an OLLAS tag to GFP and a V5 tag to mCherry enables sensitive immunostaining and immunoblotting. Reference: Doenier J, et al. RNA. 2021 Jun;(6)643-652. PMID: 33727224. Mutagen: CRISPR-Cas9 Outcrossed: x0 Made by: Jon Doenier Received: 03/03/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6154 Species: C. elegans Genotype: lst-1(q1086 [*q1004]) I. Description: 3xV5 epitope tag inserted into C-teminus of endogenous lst-1 locus with PUF-interacting motif B disrupted (PIM B mutant). Reference: Haupt KA, et al. 2019 Oct 17;146(20):dev181644. PMID: 31515205. Mutagen: Crispr/Cas9 Outcrossed: x Made by: A. Ferdous Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6179 Species: C. elegans Genotype: gld-2(q1100[gld-2::2xV5]) I. Description: 2xV5 tag inserted into endogenous gld-2 locus via CRISPR/Cas9 engineering. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Peggy Kroll-Conner Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6188 Species: C. elegans Genotype: lst-1(q1115[*q1004]) I. Description: GGSGG linker::3xV5 epitope tag inserted into C-teminus of endogenous lst-1 locus with a deletion removing a portion of the lst-1 locus. q1115 retains the N-terminal 1-210) region of LST-1. Reference: Haupt KA, et al. 2019 Oct 17;146(20):dev181644. PMID: 31515205. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Mike Green Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6203 Species: C. elegans Genotype: lst-1(q1125[*q1086]) I. Description: 3xV5 epitope tag inserted into C-teminus of endogenous lst-1 locus with PUF-interacting motifs A&B disrupted (PIM AB mutant). Reference: Haupt KA, et al. 2019 Oct 17;146(20):dev181644. PMID: 31515205. Mutagen: Crispr/Cas9 Outcrossed: x Made by: K. Haupt Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6251 Species: C. elegans Genotype: apx-1(q1148[apx-1::3xV5]) V. Description: CRISPR/Cas9 gene editing was used to insert a 3xV5 tag inserted at the C-terminus of the endogenous apx-1 locus immediately upstream of STOP codon. Animals are fertile and express low levels of APX-1 V5 in adult DTCs. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Sarah Crittenden Received: 09/11/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6268 Species: C. elegans Genotype: qSi380 IV. Description: qSi380 [mex-5p::eGFP::3xOLASS::linker::his-58::MODC pest::3xboxb::tbb-2 3’utr::SL2 trans-splice site::mCherry::3xV5::linker::his-58::MODC pest::mutant 3xboxb::tbb-2 3'utr::tbb-1 intergenic region] IV. Worms are fertile at 20C. Improved tethering assay for use in the C. elegans germline. GFP reporter mRNA is under control of a germline-expressed mex-5 promoter and has three boxB stem–loops in its 3′UTR. The RNA-binding protein (RBP) is tagged with lamda-N. The nascent transcript driven by mex-5 promoter is resolved by trans-splicing into two mRNAs that encode distinct reporters. The gfp reporter RNA has three functional boxB stem–loops in its 3′UTR; the mCherry reporter 3′UTR has three mutated boxB stem–loops that do not bind lamda-N and therefore provides an internal control. Addition of an OLLAS tag to GFP and a V5 tag to mCherry enables sensitive immunostaining and immunoblotting. Reference: Doenier J, et al. RNA. 2021 Jun;(6)643-652. PMID: 33727224. Mutagen: MosSCI Outcrossed: x0 Made by: Jonathan Doenier Received: 01/18/23 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6280 Species: C. elegans Genotype: puf-11(q1128[3xV5::puf-11]) IV. Description: 3x V5 epitope tags inserted into endogenous puf-11 locus. Expresses functional 3x V5-tagged PUF-11. Reference: Haupt KA, Genetics. 2020 Jan;214(1):147-161. PMID: 31740451 Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Kimberly Haupt Received: 05/18/21 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6319 Species: C. elegans Genotype: lst-1(q1004[lst-1::3xV5]) sygl-1(q828) I. Description: 3xV5 epitope tag inserted into C-teminus of endogenous lst-1 locus. Mutagen: N/A Outcrossed: x Made by: A. Enright Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6321 Species: C. elegans Genotype: puf-3(q966) puf-11(q971) IV/ nT1[qIs51] (IV;V). Description: Homozygous maternal effect lethal double mutant balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP+, arrested GFP+ nT1[qIs51] aneuploids, and non-GFP puf-3(q966) puf-11(q971) homozygotes (maternal effect lethal). Homozygous nT1[qIs51] inviable. Pick wild-type GFP and check for correct segregation of progeny to maintain. Mutagen: Outcrossed: x0 Made by: Kim Haupt Received: 01/22/20 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK633 Species: Caenorhabditis elegans Genotype: unc-36(e873)/unc-32(e189) glp-1(q46) III. Description: Heterozygotes are WT and segregate WT, Unc-36 and UncSteriles and dead eggs. e873 is aka eT1. Maintain by picking WT. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x>3 Made by: Judith Kimble Received: 02/22/88 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6381 Species: C. elegans Genotype: lst-1(q1124[*q1004]). Description: 3xV5 epitope tag inserted into C-teminus of endogenous lst-1 locus with PUF-interacting motif A disrupted (PIM A mutant). Reference: Haupt KA, et al. 2019 Oct 17;146(20):dev181644. PMID: 31515205. Mutagen: Crispr/Cas9 Outcrossed: x Made by: A. Ferdous Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6383 Species: C. elegans Genotype: mpk-1(q1147[V5::mpk-1B] q1183[mpk-1AB::2xOLLAS]) III. Description: Endogenous mpk-1 locus tagged with a single V5 tag inserted into the mpk-1b-specific exon to specifically label the N-terminus of the MPK-1B protein, and two tandem OLLAS tags inserted into the C-terminus, labeling both MPK-1A and MPK-1B isoforms. Reference: Robinson-Thiewes et al. Cell Reports, In Press. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Sarah Robinson-Thiewes Received: 07/26/21 Universite du Quebec a Trois-Rivieres, Quebec, Canada ----------------------------------------------------------------------------- Strain: JK6389 Species: C. elegans Genotype: sygl-1(q1167[*q1135])) I. Description: C-teminal V5 epitope tag inserted into endogenous sygl-1 locus that has a CRISPR-engineered mutation of predicted Notch-dependent cis-regulatory elements LBS BCD (Yoo et al., 2004). Reference: Lynch TR, et al. Development. 2022 Apr 1;149(7):dev200332. PMID: 35394007. Mutagen: Crispr/Cas9 Outcrossed: x Made by: T. Lynch Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6399 Species: C. elegans Genotype: lst-1(q1198[*q867]) I. Description: 3xV5 epitope tag inserted into endogenous lst-1 locus with a 1 bp deletion in lst-1L-specific first exon tospecifically disrupt LST-1L isoform. Synthetically sterile in combination with sygl-1(lf). Reference: Haupt KA, et al. 2019 Oct 17;146(20):dev181644. PMID: 31515205. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Amy Enright Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6401 Species: C. elegans Genotype: lst-1(q869) sygl-1(q828) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Pick GFP+ to maintain. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP q869 q828 homozygotes (sterile). Homozygous hT2[bli-4 let-? qIs48] inviable. Maintain by picking GFP+ heterozygotes and checking for correct segregation of progeny to maintain a balanced stock. Reference: Lynch TR, et al. Development. 2022 Apr 1;149(7):dev200332. PMID: 35394007. Mutagen: N/A Outcrossed: x Made by: T. Lynch Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6403 Species: C. elegans Genotype: mpk-1(q1147[V5::mpk-1B] q1201[mpk-1B del] q1183[mpk-1AB::2xOLLAS])/qC1 [qIs56] III. Description: qIs56 [lag-2p::GFP + unc-119(+)]. q1201 is a 125 bp deletion causing a frameshift in mpk-1B without affected mpk-1A. Heterozygous animals Roll and have GFP+ distal tip cells. Segregates roller GFP(+) heterozygotes and non-roller GFP(-) mpk-1 homozygotes (sterile, but form a vulva). qC1 [dpy-19(e1259) glp-1(q339) qIs26] homozygotes are not viable. Endogenous mpk-1 locus tagged with a single V5 tag inserted into the mpk-1b-specific exon to specifically label the N-terminus of the MPK-1B protein, and two tandem OLLAS tags inserted into the C-terminus, labeling both MPK-1A and MPK-1B isoforms. Reference: Robinson-Thiewes et al. Cell Reports, In Press. Mutagen: Outcrossed: x2 Made by: Sarah Robinson-Thiewes Received: 07/26/21 Universite du Quebec a Trois-Rivieres, Quebec, Canada ----------------------------------------------------------------------------- Strain: JK6410 Species: C. elegans Genotype: fbf-2(q1078[*q945]) II. Description: q1078 is an engineered insertion of LambdaN peptide near the C-terminus of 3xFLAG-tagged FBF-2. Derived by modification of parental strain JK5810 fbf-2(q945[3xFLAG::fbf-2]) II. Reference: Carrick BH, et al. Dev Cell. 2024 Mar 11;59(5):661-675.e7. doi: 10.1016/j.devcel.2024.01.005. PMID: 38290520. Mutagen: CRISPR Outcrossed: x4 Made by: Peggy Kroll-Conner/Brian Carrick Received: 05/31/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6412 Species: C. elegans Genotype: fbf-2(q1080[*1011]) II. Description: q1080 is an engineered insertion of LambdaN peptide near the C-terminus of 3xFLAG-tagged FBF-2 with a Y479A point mutation in the R7/R8 loop. Derived by modification of parental strain JK5984 fbf-2(q1011[*q945]) II. Reference: Carrick BH, et al. Dev Cell. 2024 Mar 11;59(5):661-675.e7. doi: 10.1016/j.devcel.2024.01.005. PMID: 38290520. Mutagen: CRISPR Outcrossed: x4 Made by: Peggy Kroll-Conner/Brian Carrick Received: 05/31/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6432 Species: C. elegans Genotype: mpk-1(q1190)/qC1 [dpy-19(e1259) glp-1(q339) qIs26] III. Description: Heterozygous animals Roll and have GFP(+) distal tip cells. Segregates roller GFP(+) heterozygotes and non-roller GFP(-) mpk-1 homozygotes which are sterile and vulvaless. qC1 [dpy-19(e1259) glp-1(q339) qIs26] homozygotes are not viable. q1190 is a deletion in mpk-1 that removes 2221bp between axons 2-7 (based on mpk-1b annotation). Sequence is shared between mpk-1a and mpk-1b. The deletion is in frame and leaves 27bp of coding sequence.Reference: Robinson-Thiewes S, et al. Cell Rep. 2021 May 25;35(8):109162. doi: 10.1016/j.celrep.2021.109162. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Sarah Robinson-Thiewes Received: 11/15/21 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6468 Species: C. elegans Genotype: gld-3(q1215[*q1065]) II. Description: 1xV5 tag inserted into endogenous gld-3 locus containing K864A & L867A engineered mutations, specifically tagging GLD-3L isoform. GLD-3L::1xV5 (K864A, L867A) animals are 30% Fog. Derived by CRISPR-engineered mutation of parental strain JK6091. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Brian Carrick Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6509 Species: C. elegans Genotype: fbf-1(q1227) fbf-2(q945[3xFLAG::fbf-2])/mIn1 [mIs14 dpy-10(e128)] II. Description: Pick wild-type GFP+ to maintain. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ (heterozygotes), Dpy bright GFP+ (mIn1 homozygotes), and non-GFP fbf-1 fbf-2 homozygotes. fbf-1(q1227) fbf-2(q945[3xFLAG::fbf-2]) homozygotes are partially sterile, ~50% make excess sperm and delay oogenesis resulting in delayed egg laying when compared to wild-type animals. Pick WT dim GFP and check for correct segregation of progeny to maintain. q1227 is an engineered Y477A point mutation in FBF-1 derived by modification of parental strain JK5810. Mutagen: Crispr/Cas9 Outcrossed: x6 Made by: Brian Carrick Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6510 Species: C. elegans Genotype: fbf-1(q1228) fbf-2(q1011[*q945])/mIn1 [mIs14 dpy-10(e128)] II. Description: Pick wild-type GFP+ to maintain. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ (heterozygotes), Dpy bright GFP+ (mIn1 homozygotes), and non-GFP fbf-1 fbf-2 homozygotes (sterile). Pick WT dim GFP and check for correct segregation of progeny to maintain. q1011 is an engineered Y479A point mutation in the R7/R8 loop of 3xFLAG-tagged FBF-2 derived by modification of parental strain JK5810 fbf-2(q945[3xFLAG::fbf-2]) II. q1228 is an engineered Y477A point mutation in FBF-1 derived by modification of parental strain JK5984. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Brian Carrick Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6516 Species: C. elegans Genotype: lst-1(q869) sygl-1(q1054) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Pick GFP+ to maintain. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP q869 q1054 homozygotes (sterility/reduced fertility). Homozygous hT2[bli-4 let-? qIs48] inviable. Maintain by picking GFP+ heterozygotes and checking for correct segregation of progeny to maintain a balanced stock. Reference: Lynch TR, et al. Development. 2022 Apr 1;149(7):dev200332. PMID: 35394007. Mutagen: N/A Outcrossed: x Made by: T. Lynch Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6526 Species: C. elegans Genotype: let-711(q1238[let-711::3xV5]) III. Description: GSS linker and 3xV5 tag inserted at C-teminus of endogenous let-711 locus. Generated in N2 background. Reference: Carrick BH, et al. Dev Cell. 2024 Mar 11;59(5):661-675.e7. doi: 10.1016/j.devcel.2024.01.005. PMID: 38290520. Mutagen: CRISPR Outcrossed: x2 Made by: Ahlan Ferdous Received: 05/31/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6531 Species: C. elegans Genotype: gld-1(q1234) I. Description: CRIPSR-engineered modification of gld-1 FBEa* in gld-1 3'UTR.  Homozygotes are fertile with a slight increase in distal GLD-1 protein levels. Reference: Qiu et al., In preparation. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK654 Species: Caenorhabditis elegans Genotype: fem-3(q23) IV. Description: Maintain at 15C. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 04/11/08 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6540 Species: C. elegans Genotype: gld-1(q1242) I. Description: q1242 is an engineered TGT to ACA substitution in FBEa1 of the endogenous gld-1 locus with a downstream G to C substitution to facilitate screening by restriction digest. Reference: Carrick BH, et al. Dev Cell. 2024 Mar 11;59(5):661-675.e7. doi: 10.1016/j.devcel.2024.01.005. PMID: 38290520. Mutagen: CRISPR Outcrossed: x2 Made by: Sarah Crittenden Received: 05/31/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6541 Species: C. elegans Genotype: gld-1(q1243) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Pick GFP+ to maintain. CRIPSR-engineered modification of gld-1 FBEa and FBEa* in gld-1 3'UTR.  Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP q1243 homozygotes (sterility/reduced fertility). Homozygous hT2[bli-4 let-? qIs48] inviable. Maintain by picking GFP+ heterozygotes and checking for correct segregation of progeny to maintain a balanced stock. Increase in distal GLD-1 protein levels and decrease in proximal GLD-1 protein levels. Qiu et al., in preparation. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Sarah Crittenden Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6547 Species: C. elegans Genotype: fbf-1(q1250) fbf-2(q738)/mIn1 [mIs14 dpy-10(e128)] II. Description: Pick wild-type GFP+ to maintain. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ (heterozygotes), Dpy bright GFP+ (mIn1 homozygotes), and non-GFP fbf-1 fbf-2 homozygotes (sterile). Pick WT dim GFP and check for correct segregation of progeny to maintain. q1250 is an engineered Y477A point mutation in FBF-1 derived by modification of parental strain JK3101. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Brian Carrick Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6548 Species: C. elegans Genotype: fbf-1(q1251) fbf-2(q738)/mIn1 [mIs14 dpy-10(e128)] II. Description: Pick wild-type GFP+ to maintain. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ (heterozygotes), Dpy bright GFP+ (mIn1 homozygotes), and non-GFP fbf-1 fbf-2 homozygotes (Mog). Pick WT dim GFP and check for correct segregation of progeny to maintain. q1251 is an engineered H324A point mutation in FBF-1 derived by modification of parental strain JK3101. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Brian Carrick Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6550 Species: C. elegans Genotype: fbf-2(q1264[*q1011])/mIn1 [mIs14 dpy-10(e128)] II. Description: Pick wild-type GFP+ to maintain. 3xFlag tag inserted into endogenous fbf-2 locus with engineered (H326A, Y479A) substitutions. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ (heterozygotes), Dpy bright GFP+ (mIn1 homozygotes), and non-GFP fbf-2 homozygotes (sterile?). Pick WT dim GFP and check for correct segregation of progeny to maintain. Mutagen: Crispr/Cas9 Outcrossed: x12 Made by: Brian Carrick Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6563 Species: C. elegans Genotype: daz-1(q1254[1xV5::daz-1]) II. Description: 1xV5 tag inserted at N-teminus of endogenous daz-1 locus. Generated in N2 background. Reference: Carrick BH, et al. Dev Cell. 2024 Mar 11;59(5):661-675.e7. doi: 10.1016/j.devcel.2024.01.005. PMID: 38290520. Mutagen: CRISPR Outcrossed: x2 Made by: Brian Carrick Received: 05/31/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6568 Species: C. elegans Genotype: gld-1(q1257) I. Description: q1257 is an engineered TGT to ACA substitution in FBEb of the endogenous gld-1 locus. Reference: Carrick BH, et al. Dev Cell. 2024 Mar 11;59(5):661-675.e7. doi: 10.1016/j.devcel.2024.01.005. PMID: 38290520. Mutagen: CRISPR Outcrossed: x2 Made by: Sarah Crittenden Received: 05/31/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6578 Species: C. elegans Genotype: fbf-1(ok91) fbf-2(q1261[*q973])/mIn1 [mIs14 dpy-10(e128)] II. Description: Pick wild-type GFP+ to maintain. 3xFlag tag inserted into endogenous fbf-2 locus with engineered (H453A H454A E457A) substitutions. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ (heterozygotes), Dpy bright GFP+ (mIn1 homozygotes), and non-GFP fbf-1 fbf-2 homozygotes (Sterile). Pick WT dim GFP and check for correct segregation of progeny to maintain. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Brian Carrick Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK659 Species: Caenorhabditis elegans Genotype: mog-3(q74)/unc-93(e1500) dpy-17(e164) III. Description: Heterozygote. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Received: 10/13/94 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6593 Species: C. elegans Genotype: fbf-2(q1262[*q945]) II. Description: 3xFlag tag inserted into endogenous fbf-2 locus with engineered (H453A H454A E457A) substitutions. Mutagen: Crispr/Cas9 Outcrossed: x4 Made by: Brian Carrick Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6594 Species: C. elegans Genotype: ife-3(q1259[1xV5::ife-3]) V. Description: 1xV5 tag inserted at N-teminus of endogenous ife-3 locus. Generated in N2 background. Reference: Carrick BH, et al. Dev Cell. 2024 Mar 11;59(5):661-675.e7. doi: 10.1016/j.devcel.2024.01.005. PMID: 38290520. Mutagen: CRISPR Outcrossed: x2 Made by: Brian Carrick Received: 05/31/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6596 Species: C. elegans Genotype: fbf-1(ok91) fbf-2(q1272[*q1023])/mIn1 [mIs14 dpy-10(e128)] II. Description: Pick wild-type GFP+ to maintain. 3xFlag tag inserted into endogenous fbf-2 locus with engineered (H453A H454A E457A Y479A) substitutions. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ (heterozygotes), Dpy bright GFP+ (mIn1 homozygotes), and non-GFP fbf-1 fbf-2 homozygotes (Sterile). Pick WT dim GFP and check for correct segregation of progeny to maintain. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Brian Carrick Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6600 Species: C. elegans Genotype: lst-1(q869) sygl-1(q1167) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Pick GFP+ to maintain. C-teminal V5 epitope tag inserted into endogenous sygl-1 locus that has a CRISPR-engineered mutation of predicted Notch-dependent cis-regulatory elementa LBS BCD (Yoo et al., 2004). Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP q869 q1167 homozygotes (sterility/reduced fertility). Homozygous hT2[bli-4 let-? qIs48] inviable. Maintain by picking GFP+ heterozygotes and checking for correct segregation of progeny to maintain a balanced stock. Reference: Lynch TR, et al. Development. 2022 Apr 1;149(7):dev200332. PMID: 35394007. Mutagen: N/A Outcrossed: x Made by: M. Xue Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6602 Species: C. elegans Genotype: gld-1(q1271[*q1242]) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Engineered TGT to ACA substitutions in FBEa1 and FBEb of the endogenous gld-1 locus with a downstream G to C substitution to facilitate screening by restriction digest. Pick GFP+ to maintain. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP+, arrested hT2 aneuploids, and non-GFP q1271 homozygotes (sterile Mog). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP+ and check for correct segregation of progeny to maintain. Derived by modification of gld-1(q1242) homozygotes from parental strain JK6540. Reference: Carrick BH, et al. Dev Cell. 2024 Mar 11;59(5):661-675.e7. doi: 10.1016/j.devcel.2024.01.005. PMID: 38290520. Mutagen: CRISPR Outcrossed: x2 Made by: Sarah Crittenden Received: 05/31/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6607 Species: C. elegans Genotype: fbf-1(ok91) fbf-2(q1263[*q973])/mIn1 [mIs14 dpy-10(e128)] II. Description: Pick wild-type GFP+ to maintain. 3xFlag tag inserted into endogenous fbf-2 locus with engineered Y479F substitution. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ (heterozygotes), Dpy bright GFP+ (mIn1 homozygotes), and non-GFP fbf-2 homozygotes (sterile?). Pick WT dim GFP and check for correct segregation of progeny to maintain. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Brian Carrick Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6658 Species: C. elegans Genotype: fbf-1(ok91) fbf-2(q1285[*q1261])/mIn1 [mIs14 dpy-10(e128)] II. Description: Pick wild-type GFP+ to maintain. 3xFlag tag inserted into endogenous fbf-2 locus with engineered (N415A Y416A Q419A S453A H454A E457A) substitutions. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ (heterozygotes), Dpy bright GFP+ (mIn1 homozygotes), and non-GFP fbf-1 fbf-2 homozygotes (Mog). Pick WT dim GFP and check for correct segregation of progeny to maintain. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Brian Carrick Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6673 Species: C. elegans Genotype: fzr-1(q1290[3xV5::fzr-1]) II. Description: Endogenous fzr-1 locus tagged with 3xV5 close to the N-terminus. Insertion site is a few amino acids downstream of start site (...PAN-3xV5-SPA…). Primer sequences to validate the strain: slc316 GCTTTTGCGTGTTCTCCTCA, slc317 TGAATCCTGAGTCATCATCCGAGT, WT product 347 bp, q1290[3xV5::fzr-1] product 485 bp. Mutagen: Crispr/Cas9 Outcrossed: x2 Made by: Sarah Crittenden Received: 11/30/22 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6678 Species: C. elegans Genotype: fbf-1(ok91) fbf-2(q1291[*q973])/mIn1 [mIs14 dpy-10(e128)] II. Description: Pick wild-type GFP+ to maintain. 3xFlag tag inserted into endogenous fbf-2 locus with engineered Y479E substitution. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP+ (heterozygotes), Dpy bright GFP+ (mIn1 homozygotes), and non-GFP fbf-2 homozygotes (sterile?). Pick WT dim GFP and check for correct segregation of progeny to maintain. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Brian Carrick Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6690 Species: C. elegans Genotype: qSi422 [*rajSi50] II. Description: qSi422 [*rajSi50 (gld-1p::GFP::H2B::gld-1 3'UTR [FBEa TGT to ACA] + Cbr-unc-119(+))] II. Maintain at 24C on OP50. Select well-fed adult animals with bright germline GFP in nuclei to propagate strain. Engineered TGT to ACA substitution in FBEa in rajSi50 gld-1 3’UTR reporter and substitution of downstream G to C to disrupt PAM site. GFP is visible in germline nuclei. Kimble lab crossed original NIK50 strain with TX189 [oma-1::GFP] and back out again to reduce GFP silencing. Primers to confirm FBEa mutation: slc314 GTCACCAAGTACACTTCCAGCAAG / prHJS401 TGGCAACATGATGTATCGCTGT (~100 bp band in mutant, no product in wild-type). Reference: Carrick BH, et al. Dev Cell. 2024. "PUF partner interactions at a conserved interface shape the RNA-binding landscape and cell fate in Caenorhabditis elegans." Mutagen: CRISPR Repair template, mutations in upp Outcrossed: x4 Made by: Sarah Crittenden Received: 02/06/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6691 Species: C. elegans Genotype: qSi423 II. Description: qSi423 [gld-1p::GFP::H2B::gld-1 3'UTR(FBEa TGT to ACA & FBEa* TGT to ACA)[*rajSi50] + Cbr-unc-119(+)] II. Modification of gld-1 FBEa and FBEa* in gld-1 3'UTR of single-copy insertion transgene.  Qiu et al., in preparation. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Sarah Crittenden Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6692 Species: C. elegans Genotype: qSi424 [*rajSi50] II. Description: qSi424 [*rajSi50 (gld-1p::GFP::H2B::gld-1 3'UTR [FBEb TGT to ACA] + Cbr-unc-119(+))] II. Maintain at 24C on OP50. Select well-fed adult animals with bright germline GFP in nuclei to propagate strain. Engineered TGT to ACA substitution in FBEb in rajSi50 gld-1 3’UTR reporter and GFP is visible in germline nuclei. Kimble lab crossed original NIK50 strain with TX189 [oma-1::GFP] and back out again to reduce GFP silencing. Primers to confirm FBEb mutation: slc314 GTCACCAAGTACACTTCCAGCAAG / slc302 GGGTTAGCGTTAAGATAACTGT (~500 bp band in FBEb mutant, no product in wild-type). Reference: Carrick BH, et al. Dev Cell. 2024. "PUF partner interactions at a conserved interface shape the RNA-binding landscape and cell fate in Caenorhabditis elegans." Mutagen: CRISPR Repair template, mutations in upp Outcrossed: x6 Made by: Sarah Crittenden Received: 02/06/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6693 Species: C. elegans Genotype: qSi425 [*rajSi50] II. Description: qSi425 [*rajSi50 (gld-1p::GFP::H2B::gld-1 3'UTR [FBEa TGT to ACA] [FBEb TGT to ACA] + Cbr-unc-119(+))] II. Maintain at 24C on OP50. Select well-fed adult animals with bright germline GFP in nuclei to propagate strain. qSi425 contains engineered TGT to ACA substitution in FBEa in rajSi50 gld-1 3’UTR reporter and substitution of downstream G to C to disrupt PAM site, and TGT to ACA substitution in FBEb in rajSi50 gld-1 3’UTR reporter. GFP is visible in germline nuclei. Derived by targeted modification of FBEb in parental strain JK6690. Kimble lab crossed original NIK50 strain with TX189 [oma-1::GFP] and back out again to reduce GFP silencing. Primers to confirm FBEa mutation: slc314 GTCACCAAGTACACTTCCAGCAAG / prHJS401 TGGCAACATGATGTATCGCTGT (~100 bp band in FBEa mutant, no product in wild-type). Primers to confirm FBEb mutation: slc314 GTCACCAAGTACACTTCCAGCAAG / slc302 GGGTTAGCGTTAAGATAACTGT (~500 bp band in FBEb mutant, no product in wild-type). Reference: Carrick BH, et al. Dev Cell. 2024. "PUF partner interactions at a conserved interface shape the RNA-binding landscape and cell fate in Caenorhabditis elegans." Mutagen: CRISPR was used to mutate FBEb in a stra Outcrossed: x6 Made by: Sarah Crittenden Received: 02/06/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6694 Species: C. elegans Genotype: rajSi50 II; unc-119(ed3) III. Description: rajSi50 [gld-1p::GFP::H2B::gld-1 3'UTR + Cbr-unc-119(+)] II. Maintain at 24C on OP50. Select well-fed adult animals with bright germline GFP in nuclei to propagate strain. GFP is visible in germline nuclei, low in distal germ cells, increases proximally, strong in oocytes. Kimble lab crossed original NIK50 strain with TX189 [oma-1::GFP] and back out again to reduce GFP silencing. Primers to confirm FBEa: slc314 GTCACCAAGTACACTTCCAGCAAG / slc311 TGGCAACATGATGTATGGCACA (100 bp band in FBEa wt). FBEb: slc314 GTCACCAAGTACACTTCCAGCAAG / slc304 GGGTTAGCGTTAAGATAACACA (~500 bp band in FBEb wt). References: Theil K, et al. Nature Commun. 2019 Sep 16;10(1):4205. doi: 10.1038/s41467-019-12050-7. PMID: 31527589. Carrick BH, et al. Dev Cell. 2024. "PUF partner interactions at a conserved interface shape the RNA-binding landscape and cell fate in Caenorhabditis elegans." Mutagen: none Outcrossed: x4 Made by: Kathrin Theil Received: 02/06/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6721 Species: C. elegans Genotype: lst-1(q1086) sygl-1(q828) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Pick GFP+ to maintain. PUF-interacting motif B (PIM B) disrupted in endogenous lst-1 locus. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP q1086 q828 homozygotes (sterile). Homozygous hT2[bli-4 let-? qIs48] inviable. Maintain by picking GFP+ heterozygotes and checking for correct segregation of progeny to maintain a balanced stock. Mutagen: N/A Outcrossed: x Made by: Peggy Kroll-Conner Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6722 Species: C. elegans Genotype: lst-1(q1124) sygl-1(q828) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Pick GFP+ to maintain. PUF-interacting motif A (PIM A) disrupted in endogenous lst-1 locus. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP q1124 q828 homozygotes (sterile). Homozygous hT2[bli-4 let-? qIs48] inviable. Maintain by picking GFP+ heterozygotes and checking for correct segregation of progeny to maintain a balanced stock. Mutagen: N/A Outcrossed: x Made by: Peggy Kroll-Conner Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6736 Species: C. elegans Genotype: gld-1(q1297[*q1234]) I. Description: CRIPSR-engineered modification of gld-1 FBEa* and FBEb in gld-1 3'UTR.  Homozygotes are fertile and 20C. Qiu et al., in preparation. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Sarah Crittenden Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK6737 Species: C. elegans Genotype: fbf-2(q1295[*q1011]) II. Description: 3xFlag tag inserted into endogenous fbf-2 locus with engineered (S453A H454A E457A Y479A) substitutions. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Brian Carrick Received: 07/08/24 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK726 Species: Caenorhabditis elegans Genotype: tra-2(q122) II. Description: tra-2(q122) is a gain-of-function allele. Do not distribute this strain; other labs should request it directly from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: J Kimble Received: 12/04/10 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK816 Species: Caenorhabditis elegans Genotype: fem-3(q20) IV. Description: Gain of function. Temperature sensitive. XX germline makes only sperm at 25C; XX germline makes oocytes and excess sperm at 15C. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 06/01/95 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK845 Species: Caenorhabditis elegans Genotype: sog-10(q162) dpy-17(e164) III. Description: Dpy. Cold-sensitive Fog phenotype; incompletely penetrant, even at 12C. Low percentage of sterile hermaphrodites with an Ooc phenotype. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Judith Austin Received: 07/14/94 Syracuse University, Syracuse, NY ----------------------------------------------------------------------------- Strain: JK860 Species: Caenorhabditis elegans Genotype: fem-3(q20) IV; fog-2(q71) V. Description: Temperature-sensitive. Maintain at 15 C. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Judith Kimble Received: 02/17/11 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK867 Species: Caenorhabditis elegans Genotype: dpy-17(e164) ncl-1(e1865) unc-36(e251) III; qDp3 (III;f). Description: Animals which have the Duplication are Dpy. Animals which have lost the Duplication are DpyUncNcl. qDp3 homozygotes are lethal. Maintain by picking Dpy non-Unc. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Gamma Rays Outcrossed: x>3 Made by: Judith Austin Received: 02/22/88 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK892 Species: Caenorhabditis elegans Genotype: unc-36(e873)/unc-32(e189) glp-1(q231) III. Description: Heterozygotes are WT. At 25C hets segregate WT, Unc-36 and UncSterile and dead eggs. At 15C, hets segregate WT, Unc-36, dead eggs and Unc-32 (which are fertile and can be maintained as homozygous stock). e873 is aka eT1. Maintain by picking WT. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x>3 Made by: Sandra Maples Received: 02/22/88 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK907 Species: Caenorhabditis elegans Genotype: mog-4(q233)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Received: 10/13/94 University of Wisconsin, Madison ----------------------------------------------------------------------------- Strain: JK948 Species: Caenorhabditis elegans Genotype: unc-32(e189) glp-1(q231) III; sog-4(q304) V. Description: Unc. sog-4 suppresses glp-1 at or below 20C. Do not maintain above 20C. Some Glp dead embryos because suppression is incomplete. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: E. Maine Received: 07/14/94 Syracuse University, Syracuse, NY ----------------------------------------------------------------------------- Strain: JK966 Species: Caenorhabditis elegans Genotype: unc-32(e189) glp-1(q224) III; sog-5(q297) X. Description: Unc. sog-5 suppresses glp-1 at or below 20C. Stock cannot be maintain above 20C. Some dead Glp embryos on plates. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: E. Maine Received: 07/14/94 Syracuse University, Syracuse, NY ----------------------------------------------------------------------------- Strain: JK967 Species: Caenorhabditis elegans Genotype: ubr-5(q298) I; unc-32(e189) glp-1(q231) III. Description: Unc. Fertile with viable progeny at or below 20C. Glp-1 sterile at higher temperatures. No obvious visible phenotype associated with sog-1. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Eleanor Maine Received: 06/23/94 Syracuse University, Syracuse, NY ----------------------------------------------------------------------------- Strain: JK968 Species: Caenorhabditis elegans Genotype: unc-32(e189) glp-1(q231) III; sog-6(q306) IV. Description: Unc. Fertile with viable progeny at or below 20C. Glp-1 sterile at higher temperatures. No obvious visible phenotype associated with sog-6. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x2 Made by: Eleanor Maine Received: 06/23/94 Syracuse University, Syracuse, NY ----------------------------------------------------------------------------- Strain: JK987 Species: Caenorhabditis elegans Genotype: tra-2(q276)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUnc and males. The males will mate. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: Outcrossed: x Made by: Received: 05/27/93 Virginia Commonwealth University, Richmond, VA ----------------------------------------------------------------------------- Strain: JK993 Species: Caenorhabditis elegans Genotype: unc-51(e1189) fog-2(q71)/let-?(q265) V. Description: Heterozygotes are WT and segregate WT, Unc Females and Lethals. Maintain by picking WT. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x Made by: Tim Schedl Received: 12/02/88 Washington University School of Medicine, St. Louis MO, USA ----------------------------------------------------------------------------- Strain: JLF104 Species: C. elegans Genotype: zyg-9(wow12[ZF::GFP::SEC::3xFlag::zyg-9]) II; zif-1(gk117) III. Description: ZF-degron, GFP, and 3xFlag tags inserted into endogenous zyg-9 locus. No overt phenotypes in a zif-1(gk117) background. GFP fluorescence is observed in microtubules. Reference: Magescas J, et al. eLife. 2019 Jun 27;8:e47867. doi: 10.7554/eLife.47867. PMID: 31246171. Mutagen: N/A Outcrossed: x3 Made by: Jenny Zonka Received: 01/10/24 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: JLF14 Species: C. elegans Genotype: gip-1(wow3[GFP::gip-1]) III Description: GFP tag inserted into endogenous gip-1 locus. No overt phenotypes. GFP fluorescence is observed at microtubule-organizing centers. Reference: Sallee M, et al. PLoS Biol. 2018 Aug 6;16(8):e2005189. doi: 10.1371/journal.pbio.2005189. PMID: 30080857. Mutagen: N/A Outcrossed: x2 Made by: Jessica Feldman Received: 01/10/24 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: JLF145 Species: C. elegans Genotype: zif-1(gk117) III; air-1(wow14[AIR-1::ZF::GFP::3xFLAG]) V. Description: ZF-degron, GFP, and 3xFLAG tags inserted into endogenous air-1 locus. No overt phenotypes in a zif-1(gk117) background. GFP expression is observed in mitotic cells at the spindle poles and along microtubules. Presence of ZF-degron targets tagged proteins for ZIF-mediated degradation. Expression of ZIF-1 causes the tagged AIR-1 protein to be degraded. Reference: Sallee M, et al. PLoS Biol. 2018 Aug 6;16(8):e2005189. doi: 10.1371/journal.pbio.2005189. PMID: 30080857. Mutagen: N/A Outcrossed: x4 Made by: Maria Sallee Received: 01/10/24 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: JLF155 Species: C. elegans Genotype: zif-1(gk117) III. Description: Presumptive null deletion allele of zif-1. ZF1-tagged proteins are not degraded in zif-1(gk117) background. Genotyping primers: ExtFwd: gctcgcaacgactgacaagg // IntRev: GGTACTCGCGGAACACTCACTC // ExtRev: ATTCGTACGGTACTTGCATGAACC Mutagen: Outcrossed: x6 Made by: Jenny Zonka Received: 01/10/24 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: JLF16 Species: C. elegans Genotype: ptrn-1(wow4[ptrn-1::GFP]) X. Description: GFP tag inserted into endogenous ptrn-1 locus. No overt phenotypes. GFP fluorescence is observed at non-centrosomal microtubule-organizing centers, including the apical surface of intestinal cells. Reference: Sallee M, et al. PLoS Biol. 2018 Aug 6;16(8):e2005189. doi: 10.1371/journal.pbio.2005189. PMID: 30080857. Mutagen: N/A Outcrossed: x2 Made by: Jessica Feldman Received: 01/10/24 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: JLF173 Species: C. elegans Genotype: gip-1(wow25[tagRFP-T::3xMyc::gip-1]) zif-1(gk117) III. Description: tagRFP-T and 3xMyc tags inserted into endogenous gip-1 locus. No overt phenotypes. RFP fluorescence is observed at microtubule-organizing centers, though generally much dimmer than the GFP allele gip-1(wow3). Reference: Sallee M, et al. PLoS Biol. 2018 Aug 6;16(8):e2005189. doi: 10.1371/journal.pbio.2005189. PMID: 30080857. Mutagen: N/A Outcrossed: x2 Made by: Jenny Zonka Received: 01/10/24 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: JLF212 Species: C. elegans Genotype: par-6(wow31[par-6::ZF::GFP::3xFLAG]) I; zif-1(gk117) III. Description: ZF-degron, GFP, and 3xFLAG tags inserted into endogenous par-6 locus. No overt phenotypes in a zif-1(gk117) background. GFP fluorescence is observed at the anterior cortex in zygotes and at apical surfaces in epithelia. Presence of ZF-degron targets tagged proteins for ZIF-mediated degradation. Expression of ZIF-1 causes the tagged PAR-6 protein to be degraded. Reference: Sallee M, et al. eLife. 2021 Jun 17;10:e64437. doi: 10.7554/eLife.64437. PMID: 34137371. Mutagen: N/A Outcrossed: x4 Made by: Maria Sallee Received: 01/10/24 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: JLF238 Species: C. elegans Genotype: tpxl-1(wow34[ZF::GFP::3xFlag::tpxl-1]) I; zif-1(gk117) III. Description: ZF-degron, GFP, and 3xFLAG tags inserted into endogenous tpxl-1 locus. No overt phenotypes in a zif-1(gk117) background. GFP expression is observed in microtubules. Presence of ZF-degron targets tagged proteins for ZIF-mediated degradation. Expression of ZIF-1 causes the tagged TPXL-1 protein to be degraded. Reference: Magescas J, et al. eLife. 2019 Jun 27;8:e47867. doi: 10.7554/eLife.47867. PMID: 31246171. Mutagen: N/A Outcrossed: x3 Made by: Jenny Zonka Received: 01/10/24 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: JLF24 Species: C. elegans Genotype: gip-1(wow5[ZF::GFP::gip-1]) zif-1(gk117) III. Description: ZF-degron and GFP tags inserted into endogenous gip-1 locus. No overt phenotypes in a zif-1(gk117) background. GFP fluorescence is observed at microtubule-organizing centers. Presence of ZF-degron targets tagged proteins for ZIF-mediated degradation. Expression of ZIF-1 causes the tagged GIP-1 protein to be degraded. Reference: Sallee M, et al. PLoS Biol. 2018 Aug 6;16(8):e2005189. doi: 10.1371/journal.pbio.2005189. PMID: 30080857. Mutagen: N/A Outcrossed: x0 Made by: Jessica Feldman Received: 01/10/24 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: JLF291 Species: Caenorhabditis elegans Genotype: wowEx68. Description: wowEx68 [ges-1p::3xHA::TurboID::unc-54 3'UTR + myo-2p::mCherry]. Pick mCherry+ to maintain. TurboID with HA tag is expressed in intestinal cells. Reference: Branon TC, et al. Nat Biotechnol. 2018 Oct;36(9):880-887. Mutagen: No mutagens used Outcrossed: x0 Made by: Ariana D. Sanchez Received: 11/08/18 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: JLF294 Species: Caenorhabditis elegans Genotype: wowEx71. Description: wowEx71 [ges-1p::3xHA::miniTurbo::unc-54 3'UTR + myo-2p::mCherry]. Pick mCherry+ to maintain. miniTurbo with HA tag is expressed in intestinal cells. Reference: Branon TC, et al. Nat Biotechnol. 2018 Oct;36(9):880-887. Mutagen: Outcrossed: x0 Made by: Ariana D. Sanchez Received: 11/08/18 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: JLF302 Species: C. elegans Genotype: ebp-2(wow47[ebp-2::GFP::3xFLAG]) II; zif-1(gk117) III. Description: GFP and 3xFLAG tags inserted into endogenous ebp-2 locus. No overt phenotypes. GFP fluorescence is observed the tips of growing microtubules. Reference: Sallee M, et al. PLoS Biol. 2018 Aug 6;16(8):e2005189. doi: 10.1371/journal.pbio.2005189. PMID: 30080857. Mutagen: N/A Outcrossed: x2 Made by: Jessica Feldman Received: 01/10/24 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: JLF348 Species: C. elegans Genotype: mzt-1(wow51[GFP::3xFLAG::mzt-1]) I; zif-1(gk117) III. Description: GFP and 3xFLAG tags inserted into endogenous mzt-1 locus. No overt phenotypes. GFP fluorescence is observed at microtubule-organizing centers. Reference: Sallee M, et al. PLoS Biol. 2018 Aug 6;16(8):e2005189. doi: 10.1371/journal.pbio.2005189. PMID: 30080857. Mutagen: N/A Outcrossed: x2 Made by: Jenny Zonka Received: 01/10/24 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: JLF361 Species: C. elegans Genotype: spd-5(wow52[GFP:3xFlag:spd-5]) I. Description: GFP and 3xFLAG tags inserted into endogenous spd-5 locus. No overt phenotypes. GFP fluorescence is observed at centrosomes and ciliary base. Reference: Magescas J, et al. eLife. 2019 Jun 27;8:e47867. doi: 10.7554/eLife.47867. PMID: 31246171. Mutagen: N/A Outcrossed: x3 Made by: Jeremy Magescas Received: 01/10/24 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: JLF425 Species: C. elegans Genotype: spd-5(wow36[tagRFP-T::spd-5]) spd-2(wow60[spd-2::GFP::3xFlag]) I. Description: tagRFP-T tag inserted into endogenous spd-5 locus. GFP and 3xFLAG tags inserted into endogenous spd-2 locus. No overt phenotypes. GFP and RFP fluorescence is observed at centrosomes. Reference: Magescas J, et al. eLife. 2019 Jun 27;8:e47867. doi: 10.7554/eLife.47867. PMID: 31246171. Mutagen: N/A Outcrossed: x3 Made by: Jeremy Magescas Received: 01/10/24 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: JLFb1 Species: E. coli Genotype: E. coli [MG1655 bioB::kan]. Description: Biotin auxotrophic E. coli strain is kan resistant and grows fine on LB. This mutant strain produces significantly less biotin and can therefore be used to reduce background in TurboID experiments. Also known as MG1655 bioB::kan and STL110 (J. Cronen, U. of Illinois). References: Sanchez AD & Feldman JL. STAR Protoc. 2021 Dec 2;2(4):100986. doi: 10.1016/j.xpro.2021.100986. PMID: 34927095. Ortega-Cuellar D., et al. J Nutrigenet Nutrigenomics. 2010;3(1):18-30. doi: 10.1159/000318054. PMID: 20798549 Mutagen: N/A Outcrossed: x0 Made by: Ortega-Cuellar et al., 2010 Received: 01/10/24 Stanford University, Stanford, CA ----------------------------------------------------------------------------- Strain: JM1041 Species: Caenorhabditis elegans Genotype: ges-1(ca6ca7) V. Description: Phenotypically normal except that it does not show ges-1 activity. Mutations (ca6ca7) are presumably misense or nonsense but have not been characterized genetically. Mutagen: IEF (ca6) and EMS (ca7) Outcrossed: x5 Made by: Jim McGhee Received: 07/08/96 University of Calgary, Alberta ----------------------------------------------------------------------------- Strain: JM124 Species: Caenorhabditis elegans Genotype: elt-4(ca16) X. Description: No obvious phenotype. Chromosomal deletion beginning from -42 bp to +1196 bps relative to elt-4 ATG (+20bp insert). Mutagen: EMS Outcrossed: x7 Made by: Jim McGhee Received: 01/20/04 University of Calgary, Alberta ----------------------------------------------------------------------------- Strain: JM125 Species: C. elegans Genotype: caIs108. Description: caIs108 [ges-1p::YFP::actin + unc-119(+)]. Mutagen: gamma irradiation Outcrossed: x4 Made by: Jim McGhee Received: 07/06/21 University of Calgary, Alberta ----------------------------------------------------------------------------- Strain: JM126 Species: Caenorhabditis elegans Genotype: pho-1(ca101ca102) II. Description: Partial maternal effect lethal. Lack of PHO-1 acid phosphatase activity on isoelectric focusing gel. Mutagen: EMS Outcrossed: x4 Made by: T Fukushige/R Predy Received: 05/05/06 University of Calgary, Alberta ----------------------------------------------------------------------------- Strain: JM130 Species: Caenorhabditis elegans Genotype: pho-1(ca101ca102) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozgyotes are WT and segregate WT, DpyUncs, and Unc Pho (slightly slow growing, about 15% shorter than WT and produce about 60% inviable embryos; early broods are less viable than later broods). Mutagen: Outcrossed: x Made by: Barbara Goszczynski Received: 05/05/06 University of Calgary, Alberta ----------------------------------------------------------------------------- Strain: JM144 Species: Caenorhabditis elegans Genotype: mnDp1 (X;V)/+ V; lin-15B&lin-15A(n765) gob-1(ca17) X. Description: Animals with Dp/+ are WT. Animals which have lost the Dp arrest as L1 with Gob (gut-obstructed) phenotype. Animals with Dp/Dp are sterile homozygotes. gob-1(ca17) is a small deletion, removing nine genes between R03A10.4 and H13N06.4 (inclusive) and possibly the seven additional genes F39D8.2 to R03A10.3 and H13N05.6. Mutagen: EMS Outcrossed: x5 Made by: Jay Kormish Received: 08/07/06 University of Calgary, Alberta ----------------------------------------------------------------------------- Strain: JM149 Species: C. elegans Genotype: caIs71. Description: caIs71[elt-2p::GFP::HIS-2B::unc-54 3'UTR + rol-6(su1006)]. Rollers. Expresses nuclear-localized GFP in all intestinal nuclei under control of 5.2 kb elt-2 promoter. GFP is fused to Histone H2B + (fused pie-1 and truncated unc-54 3'-UTR). Transgene was integrated into N2 background by exposure to gamma rays. Reference: Dineen A, et al. Dev Biol. 2018 Mar 15;435(2):150-161. PMID: 29360433 Mutagen: array integrated by gamma rays Outcrossed: x4 Made by: Jim McGhee Received: 08/11/20 University of Calgary, Alberta ----------------------------------------------------------------------------- Strain: JM311 Species: C. elegans Genotype: lem-2(ca19) II. Description: Overall healthy but reduced brood size and pharyngeal pumping rate. Synthetic lethal with emr-1(-). ca19 is a Leu to Arg mutation at position 16 of LEM-2, reconstituting a mutation in the American Hutterite Population that causes juvenile cataracts and premature cardiomyopathy. Mutagen: CRISPR Outcrossed: x2 Made by: Jim McGhee Received: 06/24/20 University of Calgary, Alberta ----------------------------------------------------------------------------- Strain: JM47 Species: Caenorhabditis elegans Genotype: ges-1(ca6) V. Description: Phenotypically WT. Isoelectric variant of ges-1 intestinal carboxylesterase. Mutagen: EMS Outcrossed: x>5 Made by: Jim McGhee Received: 07/18/96 University of Calgary, Alberta ----------------------------------------------------------------------------- Strain: JM69 Species: Caenorhabditis elegans Genotype: dpy-5(e61) unc-13(e1091)/szT1 [lon-2(e678)] I; elt-2(ca15)/szT1 X. Description: Heterozygotes are WT and segregate WT, Lon males and dead eggs. Mutagen: Tc1 Outcrossed: x>3 Made by: Jim McGhee Received: 03/17/99 University of Calgary, Alberta ----------------------------------------------------------------------------- Strain: JM9 Species: Caenorhabditis elegans Genotype: ges-1(ca1) V. Description: Phenotypically WT. Isoelectric variant of ges-1 intestinal carboxylesterase. Mutagen: EMS Outcrossed: x>5 Made by: Jim McGhee Received: 07/18/96 University of Calgary, Alberta ----------------------------------------------------------------------------- Strain: JMC101 Species: C. elegans Genotype: csr-1(tor67[gfp::3xflag::csr-1]) IV . Description: GFP and 3xFLAG tags inserted into second exon of endgonenous csr-1 locus; tags both isoforms. Reference: Ouyang JPT, et al. Dev Cell. 2019 Sep 23;50(6):716-728.e6. PMID: 31402283 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Amanda Charlesworth Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC135 Species: C. elegans Genotype: vsra-1(tor94[GFP::3xFLAG::vsra-1] I. Description: GFP and 3xFLAG tags inserted into endgonenous vsra-1/C04F12.1 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Monica Wu Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC151 Species: C. elegans Genotype: csr-1(tor160[csr-1 exon1::GFP::FLAG (IV:7957568)]) IV . Description: GFP and 3xFLAG tags inserted into first exon of endgonenous csr-1 locus (IV:7957568); specifically tags a-isoform. Mutagen: Crispr/Cas9 Outcrossed: x Made by: Amanda Charlesworth Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC164 Species: C. elegans Genotype: csr-1(tor67[csr-1 exon2::GFP::FLAG IV:7958598]csr- 1(mg660[G120*]) IV) IV . Description: Null allele of csr-1 with GFP and 3xFLAG tags inserted into second exon of endgonenous csr-1 locus. tor67 would normally tag both long and short isoforms, but the mg660 allele introduces a stop codon into the first exon so the long isoform is not made and only tagged CSR-1B isoform is produced. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Amanda Charlesworth Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC192 Species: C. elegans Genotype: wago-10(tor133) V. Description: Null allele of wago-10. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC193 Species: C. elegans Genotype: sago-2(tor135) I. Description: Null allele of sago-2; detectable by PCR/restriction digest. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC201 Species: C. elegans Genotype: alg-1(tor137[GFP::3xFLAG::alg-1b]) X. Description: GFP and 3xFLAG tags inserted into endgonenous alg-1 locus; specifically tags b-isoform. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC203 Species: C. elegans Genotype: alg-2(tor139[GFP::3xFLAG::alg-2b]) II. Description: GFP and 3xFLAG tags inserted into endgonenous alg-2 locus; specifically tags b-isoform. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC205 Species: C. elegans Genotype: alg-3(tor141[GFP::3xFLAG::alg-3]) IV. Description: GFP and 3xFLAG tags inserted into endgonenous alg-3 locus. Reference: Charlesworth AG, et al. Nucleic Acids Res. 2021 Sep 7;49(15):8836-8865. PMID: 34329465 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC207 Species: C. elegans Genotype: alg-4(tor143[GFP::3xFLAG::alg-4]) III. Description: GFP and 3xFLAG tags inserted into endgonenous alg-4 locus. Reference: Charlesworth AG, et al. Nucleic Acids Res. 2021 Sep 7;49(15):8836-8865. PMID: 34329465 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC209 Species: C. elegans Genotype: alg-5(tor145[GFP::3xFLAG::alg-5]) I. Description: GFP and 3xFLAG tags inserted into endgonenous alg-5 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC211 Species: C. elegans Genotype: ergo-1(tor147[GFP::3xFLAG::ergo-1a]) V. Description: GFP and 3xFLAG tags inserted into endgonenous ergo-1 locus; specifically tags a-isoform. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC213 Species: C. elegans Genotype: prg-1(tor149[GFP::3xFLAG::prg-1]) I. Description: GFP and 3xFLAG tags inserted into endgonenous prg-1 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC217 Species: C. elegans Genotype: rde-1(tor153[GFP::3xFLAG::rde-1]) V. Description: GFP and 3xFLAG tags inserted into endgonenous rde-1 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC219 Species: C. elegans Genotype: wago-1(tor113[GFP::3xFLAG::wago-1]) I. Description: GFP and 3xFLAG tags inserted into endgonenous wago-1 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC221 Species: C. elegans Genotype: ppw-2(tor115[GFP::3xFLAG::ppw-2]) I. Description: GFP and 3xFLAG tags inserted into endgonenous ppw-2 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC223 Species: C. elegans Genotype: wago-4(tor117[GFP::3xFLAG::wago-4]) II. Description: GFP and 3xFLAG tags inserted into endgonenous wago-4 locus. Reference: Lev I, et al. Curr Biol. 2019 Sep 9;29(17):2880-2891.e4. PMID: 31378614 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC225 Species: C. elegans Genotype: ppw-1(tor119[GFP::3xFLAG::ppw-1c]) I. Description: GFP and 3xFLAG tags inserted into endgonenous ppw-1; specifically tags c-isoform. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC227 Species: C. elegans Genotype: sago-2(tor121[GFP::3xFLAG::sago-2c]) I. Description: GFP and 3xFLAG tags inserted into endgonenous sago-2; specifically tags c-isoform. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC229 Species: C. elegans Genotype: sago-1(tor123[GFP::3xFLAG::sago-1]) V. Description: GFP and 3xFLAG tags inserted into endgonenous sago-1 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC231 Species: C. elegans Genotype: hrde-1(tor125[GFP::3xFLAG::hrde-1]) III. Description: GFP and 3xFLAG tags inserted into endgonenous hrde-1 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC233 Species: C. elegans Genotype: wago-10(tor127[GFP::3xFLAG::wago-10]) V. Description: GFP and 3xFLAG tags inserted into endgonenous wago-10 locus. Reference: Charlesworth AG, et al. Nucleic Acids Res. 2021 Sep 7;49(15):8836-8865. PMID: 34329465 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC235 Species: C. elegans Genotype: wago-11(tor129[GFP::3xFLAG::wago-11]) II. Description: GFP and 3xFLAG tags inserted into endgonenous wago-11 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC237 Species: C. elegans Genotype: nrde-3(tor131[GFP::3xFLAG::nrde-3]) X. Description: GFP and 3xFLAG tags inserted into endgonenous nrde-3 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC245 Species: C. elegans Genotype: alg-4(tm1184) III; csr-1(tor67[csr-1 exon2::gfp::3xflag (IV:7958598)], csr-1(mg660[G120*])) alg-3(tm1155) IV; wago-10(tor133) V. Description: Quadruple mutant of four spermatogenesis-specific ago genes. Reference: Charlesworth AG, et al. Nucleic Acids Res. 2021 Sep 7;49(15):8836-8865. PMID: 34329465 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Amanda Charlesworth Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC247 Species: C. elegans Genotype: nrde-3(tor134[3xFLAG::nrde-3]) X. Description: 3xFLAG tag inserted into endgonenous nrde-3 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC248 Species: C. elegans Genotype: wago-10(tor162[3xFLAG::wago-10] V. Description: 3xFLAG tag inserted into endgonenous wago-10 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC273 Species: C. elegans Genotype: wago-1(tor163[3xFLAG::wago-1] Description: 3xFLAG tag inserted into endgonenous wago-1 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC288 Species: C. elegans Genotype: wago-5(tor166[GFP::3xFLAG::wago-5]) II. Description: GFP and 3xFLAG tags inserted into endgonenous wago-5 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JMC327 Species: C. elegans Genotype: vsra-1(tor170[3xflag::vsra-1]) I. Description: 3xFLAG tag inserted into endgonenous vsra-1/C04F12.1 locus. Reference: Seroussi U, et al. bioRxiv 2022.08.08.502013; doi: https://doi.org/10.1101/2022.08.08.502013 Mutagen: Crispr/Cas9 Outcrossed: x Made by: Uri Seroussi Received: 09/07/22 University of Toronto, Toronto, Ontario, Canada ----------------------------------------------------------------------------- Strain: JN1071 Species: Caenorhabditis elegans Genotype: snet-1(pe1063) X. Description: Suppresses nep-2 olfactory plasticity defect. Reference: Science. 2010 Sep 24;329(5999):1647-50. Mutagen: EMS Outcrossed: x5 Made by: Koji Yamada Received: 05/13/11 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN1194 Species: Caenorhabditis elegans Genotype: gcy-14(pe1102) V. Description: Reference: Ortiz CO, et al., Curr Biol. 2009 Jun 23;19(12):996-1004. Mutagen: Outcrossed: x4 Made by: Jun Takayama Received: 02/07/11 Columbia University, New York, NY ----------------------------------------------------------------------------- Strain: JN1209 Species: Caenorhabditis elegans Genotype: pitp-1(pe1209) III. Description: Mos1 insertion. Salt chemotaxis learning defective. Reference: Proc Natl Acad Sci U S A. 2011 May 3;108(18):7589-94. Mutagen: Mos1 Outcrossed: x6 Made by: Ryo Iwata Received: 05/13/11 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN1239 Species: C. elegans Genotype: daf-18(pe407) IV. Description: pe407 suppresses the defects in salt-starve associative learning of casy-1(tm718). Reference: Ohno H, et al. Science. 2014 Jul 18;345(6194):313-7. PMID: 25035490 Mutagen: EMS Outcrossed: x9 Made by: Ohno H. Received: 03/09/22 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN1240 Species: C. elegans Genotype: plc-1(pe1238) X. Description: Presumptive null allele of plc-1. Shows a preference for low salt concentrations. Reference: Kunitomo H, et al., 2013, Nat Commun. 2013;4:2210. doi: 10.1038/ncomms3210. Mutagen: EMS Outcrossed: x6 Made by: Iwata R. Received: 03/09/22 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN1297 Species: Caenorhabditis elegans Genotype: pitp-1(pe1297) III. Description: 1953 bp deletion. Salt chemotaxis learning defective. Reference: Proc Natl Acad Sci U S A. 2011 May 3;108(18):7589-94. Mutagen: MosSCI Outcrossed: x1 Made by: Ryo Iwata Received: 05/13/11 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN147 Species: Caenorhabditis elegans Genotype: gap-2(tm748) X. Description: No apparent phenotype. tm748 is a UV/TMP-induced gap-2 deletion allele generated by National Bioresource Project (see http://shigen.lab.nig.ac.jp/c.elegans/index.jsp?lang=englis h for info). Mutagen: UV/TMP Outcrossed: x6 Made by: Yuichi Iino Received: 06/17/04 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN1483 Species: C. elegans Genotype: daf-18(pe407) IV. Description: pe407 suppresses the defects in salt-starve associative learning of casy-1(tm718). Reference: Ohno H, et al. Science. 2014 Jul 18;345(6194):313-7. PMID: 25035490 Mutagen: EMS Outcrossed: x9 Made by: Hayao Ohno Received: 03/09/22 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN1484 Species: C. elegans Genotype: daf-18(pe408) IV. Description: pe408 suppresses the defects in salt-starve associative learning of casy-1(tm718). Reference: Ohno H, et al. Science. 2014 Jul 18;345(6194):313-7. PMID: 25035490 Mutagen: EMS Outcrossed: x9 Made by: Ohno H. Received: 03/09/22 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN1489 Species: C. elegans Genotype: daf-2(pe1230) III. Description: Daf-c phenotype at 25C; normal development at 20C. Defects in salt-starve associative learning and olfactory learning. Reference: Ohno H, et al. Science. 2014 Jul 18;345(6194):313-7. PMID: 25035490 Mutagen: EMS Outcrossed: x4 Made by: Ohno H. Received: 03/09/22 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN1601 Species: C. elegans Genotype: lite-1(ce314) X; peIs1091. Description: peIs1091 [gcy5p::ChR2Y2 + unc-122p::mCherry]. ChR2 expression in the ASER neuron. Reference: Kunitomo H, et al., 2013, Nat Commun. 2013;4:2210. doi: 10.1038/ncomms3210. Mutagen: Outcrossed: x3 Made by: Satoh Y. Received: 03/09/22 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN1695 Species: C. elegans Genotype: lite-1(ce314) X; peIs1095. Description: peIs1095 [gcy-7p::ChR2Y2::Venus + unc-122::mCherry]. ChR2Y2 expression in ASEL. Reference: Wang L, et al. J Neurosci, 2017. 37(8): p. 2097-2111. PMID: 28126744 Mutagen: Outcrossed: x3 Made by: Kunitomo H. Received: 03/09/22 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN1709 Species: C. elegans Genotype: peIs1709. Description: peIs1709 [gpc-1p::FLAG::pab-1::sl2::NLS::GFP + unc-122p::mCherry]. FLAG-tagged poly(A)-binding protein (PAB-1) can be used for mRNA tagging. Reference: Tomioka M, et al. Nat Commun. 2016 May 20;7:11645. PMID: 27198602 Mutagen: Outcrossed: x4 Made by: Tomioka M. Received: 03/09/22 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN1710 Species: C. elegans Genotype: peIs1710. Description: peIs1710 [glr-1p::FLAG::pab-1::sl2::NLS::GFP + unc-122p::mCherry]. FLAG-tagged poly(A)-binding protein (PAB-1) can be used for mRNA tagging. Reference: Tomioka M, et al. Nat Commun. 2016 May 20;7:11645. PMID: 27198602 Mutagen: Outcrossed: x4 Made by: Tomioka M. Received: 03/09/22 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN1713 Species: Caenorhabditis elegans Genotype: peIs1713. Description: peIs1713 [sra-6p::mCasp-1 + unc-122p::mCherry]. ASH neurons are eliminated. Abnormal odor chemotaxis. Reference: Yoshida K, et al. Nat Commun. 2012 Mar 13;3:739. Mutagen: gamma irradiation for integration of Ex Outcrossed: x4 Made by: Kazushi Yoshida Received: 09/11/18 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN1715 Species: Caenorhabditis elegans Genotype: peIs1715. Description: peIs1715 [str-1p::mCasp-1 + unc-122p::GFP]. AWB neurons are eliminated. Abnormal odor chemotaxis. Reference: Yoshida K, et al. Nat Commun. 2012 Mar 13;3:739. Mutagen: UV for integration of Ex into the genome Outcrossed: x4 Made by: Kazushi Yoshida Received: 09/11/18 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN2113 Species: C. elegans Genotype: peIs2113. Description: peIs2113 [gcy-21p::mCaspase + tax-4p::NLS::YC2.60 + lin-44p::GFP]. Genetic ablation of ASG neurons by specific expression of caspase. tax-4p::YC2.60 facilitates calcium imaging. Reference: Jang MS, et al. Proc Natl Acad Sci U S A. 2019 Sep 10;116(37):18673-18683. PMID: 31455735 Mutagen: Outcrossed: x4 Made by: Jang Moon Sun Received: 03/09/22 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN213 Species: Caenorhabditis elegans Genotype: iff-1(tm483)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate sterile iff-1 homozygotes and Dumpy sterile qC1 homozygotes. Maintain by picking non-Dpy fertile heterozygotes. tm483 is a UV/TMP-induced iff-1 deletion allele generated by K. Gengyo-Ando and S. Mitani. Mutagen: UV/TMP Outcrossed: x4 Made by: Momoyo Hanazawa Received: 06/17/04 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN214 Species: Caenorhabditis elegans Genotype: iff-2(tm393)/mIn1 [dpy-10(e128) mIs14] II. Description: Heterozygotes are WT and GFP+ and segregate Dpy GFP+ and slow-growing, GFP- iff-2 homozygotes. Mutagen: UV/TMP Outcrossed: x4 Made by: Momoyo Hanazawa Received: 06/22/04 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN215 Species: Caenorhabditis elegans Genotype: iff-1(tm483) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Segregates GFP+ glowing heterozygotes and non-glowing sterile iff-1 homozygotes. tm483 is a UV/TMP-induced iff-1 deletion allele generated by K. Gengyo-Ando and S. Mitani. hT2[qIs48] homozygotes inviable. qIs48 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and a gut promoter driving GFP in the intestine. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype. Mutagen: UV/TMP Outcrossed: x4 Made by: Yuichi Iino Received: 06/17/04 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN218 Species: Caenorhabditis elegans Genotype: asb-1(tm498)/qC1 [dpy-19(e1259) glp-1(q339) qIs26] III. Description: qIs26 [lag-2::GFP + rol-6(su1006)]. Heterozygotes are Rollers and GFP+ in the distal tip cell. qIs26 was integrated into qC1 and in the process made qC1 homozygous lethal. asb-1(tm498) is homozygous sterile. Mutagen: UV/TMP Outcrossed: x8 Made by: Ichiro Kawasaki Received: 02/25/08 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN219 Species: Caenorhabditis elegans Genotype: asb-1(tm499)/qC1 [dpy-19(e1259) glp-1(q339) qIs26] III. Description: qIs26 [lag-2::GFP + rol-6(su1006)]. Heterozygotes are Rollers and GFP+ in the distal tip cell. qIs26 was integrated into qC1 and in the process made qC1 homozygous lethal. asb-1(tm499) is homozygous sterile. Mutagen: UV/TMP Outcrossed: x8 Made by: Ichiro Kawasaki Received: 02/25/08 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN2389 Species: C. elegans Genotype: lite-1(xu7) X; peIs1090. Description: peIs1090 [sra-6p::ChR2Y2]. ChR2 expression in ASH neuron. Reference: Yamada K, et al. 2012 Jul;2(7):741-51. PMID: 22870397 Mutagen: Outcrossed: x3 Made by: Kunitomo H. Received: 03/09/22 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN2411 Species: C. elegans Genotype: sinh-1(pe420) II. Description: Chemotaxis abnormality, developmental delay, small brood size.  pe420 is a 22 bp deletion in exon 1. Mutagen: CRISPR-Cas9 Outcrossed: x4 Made by: Hayao Ohno/Naoko Sakai Received: 06/21/17 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN2512 Species: C. elegans Genotype: peIs422. Description: peIs422 [gcy-5p::Downward DAG2(worm) + gcy-5p::mCherry + unc-122p::mCherry]. Transgene allows imaging of changes in the amount of diacylglycerol (DAG) in ASER neuron. Reference: Ohno H, et al. Cell Rep. 2017 Sep 5;20(10):2294-2303. PMID: 28877465 Mutagen: Outcrossed: x5 Made by: Ohno H. Received: 03/09/22 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN2513 Species: Caenorhabditis elegans Genotype: peEx2513. Description: peEx2513 [ceh36p::DownwardDAG2(worm) + unc-122p::mCherry]. The diacylglycerol reporter, DownwardDAG2, is expressed in AFD. Mutagen: - Outcrossed: x0 Made by: Manami Kanamori Received: 12/07/17 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN2514 Species: Caenorhabditis elegans Genotype: peEx2514. Description: peEx2514 [gcy-8p::DownwardDAG2(codon-optimized) + unc-122p::mCherry]. The diacylglycerol reporter, DownwardDAG2, is expressed in AFD. Mutagen: - Outcrossed: x0 Made by: Manami Kanamori Received: 12/07/17 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN2722 Species: C. elegans Genotype: daf-2(pe2722) III. Description: daf-2(pe2722) is a daf-2c-isoform specific mutation. pe2722 is a CRISPR/Cas9-engineered 41 bp deletion (ggttgatgacgatgatgagcccggcggcaggaggcagtgagcaaca) in daf-2 exon 11.5. Guide RNA sequence: gacgatgaagagcccggcgg. Reference: Nagashima T, et al. PLoS Genet. 2019 Jul 19;15(7):e1008297. PMID: 31323047 Mutagen: Outcrossed: x0 Made by: Tomioka M. Received: 03/09/22 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN3038 Species: C. elegans Genotype: qjIs11; peIs3042; peIs2100; qjIs14. Description: qjIs11 [glr-1p::SVNLS2::TagBFPsyn + ser-2(prom2)::SVNLS2::TagBFPsyn]. peIs3042 [eat-4p::svnls2::TagRFP675syn + lin-44p::GFP]. peIs2100 [H-20p::NLS4::mCherry]. qjIs14 [H20p::NLS::YC2.60]. Suitable for whole-brain calcium imaging. mCherry and YC2.60 expression in almost all head neurons. BFP and RFP are also expressed to annotate neurons. H20p is a pan-neuronal promoter expressed in almost all neurons, GLR glial cells, XXX hypodermal cells, pharyngeal gland cells and HMC cells. Reference: Toyoshima Y et al. BMC Biol. 2020 Mar 19;18(1):30. PMID: 32188430; Shioi G, et al. Genetics. 2001 Apr;157(4):1611-22. PMID: 11290717. Mutagen: Outcrossed: x0 Made by: Kanamori M. Received: 03/09/22 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN356 Species: Caenorhabditis elegans Genotype: nep-2(pe356) II. Description: Olfactory plasticity defect. Reference: Science. 2010 Sep 24;329(5999):1647-50. Mutagen: EMS Outcrossed: x5 Made by: Koji Yamada Received: 05/13/11 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN372 Species: Caenorhabditis elegans Genotype: gpc-1(pe372) X. Description: Mutagen: Outcrossed: x Made by: Received: 09/04/09 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN379 Species: Caenorhabditis elegans Genotype: nep-2(pe379) II. Description: Olfactory plasticity defect. Reference: Science. 2010 Sep 24;329(5999):1647-50. Mutagen: UV/TMP Outcrossed: x5 Made by: Koji Yamada Received: 05/13/11 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN414 Species: Caenorhabditis elegans Genotype: casy-1(pe401) II. Description: Mutagen: Outcrossed: x Made by: Received: 09/04/09 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN554 Species: Caenorhabditis elegans Genotype: dyf-11(pe554) X. Description: Deletion flanking sequence (X: 764793) AGTCAACTACTAAAAAACGT-TTTTTTT-TTTTTTCAAATTCTAGAATAAGTT (X:765504). 667 BP DELETION. 7 T insertion. Mutagen: UV/TMP Outcrossed: x4 Made by: Hirofumi Kunitomo Received: 02/25/08 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN572 Species: C. elegans Genotype: clh-1(pe572) II. Description: pe572 mutants exhibit defects in salt chemotaxis after low salt concentration with food conditioning. Reference: Park C, et al. Elife. 2021 Jan 25;10:e55701. doi: 10.7554/eLife.55701. PMID: 33492228 Mutagen: EMS Outcrossed: x6 Made by: Park C. Received: 03/09/22 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN577 Species: C. elegans Genotype: clh-1(pe577) II. Description: pe577 mutants exhibit defects in salt chemotaxis after low salt concentration with food conditioning. Reference: Park C, et al. Elife. 2021 Jan 25;10:e55701. doi: 10.7554/eLife.55701. PMID: 33492228 Mutagen: EMS Outcrossed: x6 Made by: Park C. Received: 03/09/22 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN578 Species: Caenorhabditis elegans Genotype: peIs578. Description: peIs578 [npr-9p::casp1 + npr-9p::Venus + unc-122p::mCherry]. AIB neurons are ablated by specific expression of caspase. Reference: Kunitomo H, et al., 2013, Nat Commun. 2013;4:2210. doi: 10.1038/ncomms3210. Mutagen: No mutagens used Outcrossed: x3 Made by: Hirofumi Sato Received: 11/19/21 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN579 Species: Caenorhabditis elegans Genotype: peIs579. Description: peIs579 [ttx-3p::casp1 + ttx-3p::Venus + lin-44p::GFP]. AIY neurons are ablated by specific expression of caspase. Reference: Kunitomo H, et al., 2013, Nat Commun. 2013;4:2210. doi: 10.1038/ncomms3210. Mutagen: No mutagens used Outcrossed: x3 Made by: Hirofumi Sato Received: 11/19/21 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN580 Species: Caenorhabditis elegans Genotype: peIs580. Description: peIs580 [ins-1p(short)::casp1 + ins-1p(short)::Venus + unc-122p::GFP]. AIA neurons are ablated by specific expression of caspase. Reference: Kunitomo H, et al., 2013, Nat Commun. 2013;4:2210. doi: 10.1038/ncomms3210. Mutagen: No mutagens used Outcrossed: x3 Made by: Hirofumi Sato Received: 11/19/21 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN773 Species: C. elegans Genotype: goa-1(pe773) I. Description: This strain shows a preference for high salt concentration when conditioned with food. Reference: Ohno H, et al. Cell Rep. 2017 Sep 5;20(10):2294-2303. PMID: 28877465 Mutagen: EMS Outcrossed: x6 Made by: Sakai N. Received: 03/09/22 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN785 Species: C. elegans Genotype: peIs785. Description: peIs785 [casy-1p::daf-2 E11-E11.5(+c)-E12::EGFP + casy-1p::daf-2 E11-E11.5(+c)-E12(-c)::mRFP]. peIs785 caries a daf-2 splicing reporter expressed in many neurons. Reference: Tomioka M, et al. Nat Commun. 2016 May 20;7:11645. PMID: 27198602 Mutagen: Outcrossed: x4 Made by: Tomioka M. Received: 03/09/22 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JN930 Species: C. elegans Genotype: egl-30(pe914) I. Description: Presumptive gain-of-function mutant shows defects in salt-food associative learning and olfactory learning. Reference: Tomioka M, et al. 2006 Sep 7;51(5):613-25. PMID: 16950159 Mutagen: EMS Outcrossed: x4 Made by: Adachi T. Received: 03/09/22 University of Tokyo, Tokyo, Japan ----------------------------------------------------------------------------- Strain: JNC100 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; dotSi100 II. Description: dotSi100 [T06E6.2 + unc-119(+)] II. Single copy Mos insertion. Suppresses mdf-1(gk2) sterility. Maintain under normal conditions. References: Tarailo-Graovac M, et al. Cell Cycle. 2010 Dec 15;9(24):4858-65. Tarailo-Graovac M, Chen N. G3 (Bethesda). 2012 Aug;2(8):865-71. Mutagen: MosSCI Outcrossed: x0 Made by: Maja Tarailo-Graovac Received: 08/10/12 Simon Fraser University, Burnaby, BC, Canada ----------------------------------------------------------------------------- Strain: JNC144 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; dotSi110 IV. Description: dotSi110 [T06E6.2 + unc-119(+)] IV. Single copy Mos insertion. Suppresses mdf-1(gk2) sterility. Maintain under normal conditions. References: Tarailo-Graovac M, Chen N. G3 (Bethesda). 2012 Aug;2(8):865-71. Mutagen: MosSCI Outcrossed: x0 Made by: Maja Tarailo-Graovac Received: 08/10/12 Simon Fraser University, Burnaby, BC, Canada ----------------------------------------------------------------------------- Strain: JPS271 Species: Caenorhabditis elegans Genotype: vxEx265. Description: vxEx265 [gcy-8p::ICE + myo-2p:mCherry]. Pick mCherry+ animals to maintain. Expresses human caspase (ICE) to ablate AFD neurons. Reference: Vidal-Gadea AG, et al. Elife. 2015 Jun 17;4. doi: 10.7554/eLife.07493. Mutagen: Outcrossed: x0 Made by: Andres Vidal-Gadea Received: 12/04/15 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS278 Species: Caenorhabditis elegans Genotype: vxEx277. Description: vxEx277 [mec-3p::ICE + myo-2p::mCherry]. Pick animals with mCherry expression in the pharynx to maintain the array. vxEx277 expresses a human cell death caspase (ICE) to ablate six touch neurons (ALML, ALMR, AVM, PLML, PLMR, and PVM), FLP, and PVD. Reference: Russell J, et al. Proc Natl Acad Sci U S A. 2014 Jun 3;111(22):8269-74. Mutagen: Outcrossed: x0 Made by: Josh Russell Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS282 Species: Caenorhabditis elegans Genotype: asic-1(ok415) I; vxEx282. Description: vxEx282 [WRM0621dC07 + unc-122p::GFP]. Pick GFP+ to maintain. GFP expression in coelomocytes. Fosmid rescues ok415. Reference: Russell J, et al. Proc Natl Acad Sci USA. 2014 Jun 3;111(22):8269-74. Mutagen: Outcrossed: x0 Made by: Josh Russell Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS325 Species: Caenorhabditis elegans Genotype: slo-1(js379)V; vxEx325. Description: vxEx325 [slo-1p::hslo(T352I)::mCherry::unc-54 3'UTR + myo-2p::mCherry]. Pick animals with mCherry expression in the pharynx to maintain the array. vxEx325 rescues ethanol-independent phenotypes of a slo-1(null) mutants without rescuing ethanol intoxication. vxEx325 expresses human BK channel protein (hslo(T352I)) with a C-terminal mCherry tag. Reference: Davis SJ, Scott LL, Hu K & Pierce-Shimomura JT. J Neurosci. 2014 Jul 16;34(29):9562-73. Mutagen: Outcrossed: x0 Made by: Scott Davis Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS327 Species: Caenorhabditis elegans Genotype: slo-1(js379)V; vxEx327. Description: vxEx327 [slo-1p::slo-1(T381I)::mCherry::unc-54 3'UTR + myo-2p::mCherry]. Pick animals with mCherry expression in the pharynx to maintain the array. vxEx327 rescues ethanol-independent phenotypes of a slo-1(null) mutants without rescuing ethanol intoxication. vxEx327 expresses worm BK channel protein (slo-1(T381I)) with a C-terminal mCherry tag. Reference: Davis SJ, Scott LL, Hu K & Pierce-Shimomura JT. J Neurosci. 2014 Jul 16;34(29):9562-73. Mutagen: Outcrossed: x0 Made by: Scott Davis Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS338 Species: Caenorhabditis elegans Genotype: slo-1(js379)V; vxEx338. Description: vxEx338 [slo-1p::hslo(+)::mCherry::unc-54 3'UTR + myo-2p::mCherry]. Pick animals with mCherry expression in the pharynx to maintain the array. vxEx338 rescues ethanol-independent and ethanol intoxication phenotypes of a slo-1(null) mutants. vxEx338 expresses human BK channel protein (hslo(+)) with a C-terminal mCherry tag. Reference: Davis SJ, Scott LL, Hu K & Pierce-Shimomura JT. J Neurosci. 2014 Jul 16;34(29):9562-73. Mutagen: Outcrossed: x0 Made by: Scott Davis Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS344 Species: Caenorhabditis elegans Genotype: slo-1(js379)V; vxEx344. Description: vxEx344 [slo-1p::slo-1(+)::mCherry::unc-54 3'UTR + myo-2p::mCherry]. Pick animals with mCherry expression in the pharynx to maintain the array. vxEx344 rescues ethanol-independent and ethanol intoxication phenotypes of a slo-1(null) mutants. vxEx344 expresses worm BK channel protein (slo-1(+)) with a C-terminal mCherry tag. Reference: Davis SJ, Scott LL, Hu K & Pierce-Shimomura JT. J Neurosci. 2014 Jul 16;34(29):9562-73. Mutagen: Outcrossed: x0 Made by: Scott Davis Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS346 Species: Caenorhabditis elegans Genotype: slo-1(js379)V; vxEx346. Description: vxEx346 [slo-1p::hslo(D362/367A + 5D5N)::mCherry::unc-54 3'UTR + myo-2p::mCherry]. Pick animals with mCherry expression in the pharynx to maintain the array. Partially resistant to ethanol and crooked neck phenotype. vxEx346 expresses human BK channel protein (hslo(D362/367A + 5D5N)) with compromised calcium-sensing residues and a C-terminal mCherry tag. Reference: Davis SJ, Scott LL, Hu K & Pierce-Shimomura JT. J Neurosci. 2014 Jul 16;34(29):9562-73. Mutagen: Outcrossed: x0 Made by: Scott Davis Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS350 Species: Caenorhabditis elegans Genotype: slo-1(js379)V; vxEx350. Description: vxEx350 [slo-1p::slo-1(5D5N)::mCherry::unc-54 3'UTR + myo-2p::mCherry]. Pick animals with mCherry expression in the pharynx to maintain the array. Partial crooked neck phenotype. vxEx350 expresses worm BK channel protein (slo-1(5D5N)) with purported calcium-sensing residues in the calcium bowl compromised and a C-terminal mCherry tag. Reference: Davis SJ, Scott LL, Hu K & Pierce-Shimomura JT. J Neurosci. 2014 Jul 16;34(29):9562-73. Mutagen: Outcrossed: x0 Made by: Scott Davis Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS358 Species: Caenorhabditis elegans Genotype: slo-1(js379)V; vxEx358. Description: vxEx358 [slo-1p::slo-1(D391/396A + 5D5N)::mCherry::unc-54 3'UTR + myo-2p::mCherry]. Pick animals with mCherry expression in the pharynx to maintain the array. Crooked neck phenotype. vxEx358 expresses worm BK channel protein (slo-1(D391/396A + 5D5N)) with purported calcium-sensing residues compromised and a C-terminal mCherry tag. Reference: Davis SJ, Scott LL, Hu K & Pierce-Shimomura JT. J Neurosci. 2014 Jul 16;34(29):9562-73. Mutagen: Outcrossed: x0 Made by: Scott Davis Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS360 Species: Caenorhabditis elegans Genotype: slo-1(js379)V; vxEx360. Description: vxEx360 [slo-1p::slo-1(D391/396A)::mCherry::unc-54 3'UTR + myo-2p::mCherry]. Pick animals with mCherry expression in the pharynx to maintain the array. Partially crooked neck phenotype. vxEx360 expresses worm BK channel protein (slo-1(D391/396A)) with purported calcium-sensing residues in the RCK1 domain compromised and a C-terminal mCherry tag. Reference: Davis SJ, Scott LL, Hu K & Pierce-Shimomura JT. J Neurosci. 2014 Jul 16;34(29):9562-73. Mutagen: Outcrossed: x0 Made by: Scott Davis Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS383 Species: Caenorhabditis elegans Genotype: slo-1(js379)V; vxEx383. Description: vxEx383 [myo-2p::mCherry]. Pick animals with mCherry expression in the pharynx to maintain the array. Resistant to the effects of ethanol. Reference: Davis SJ, Scott LL, Hu K & Pierce-Shimomura JT. J Neurosci. 2014 Jul 16;34(29):9562-73. Mutagen: Outcrossed: x0 Made by: Scott Davis Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS428 Species: Caenorhabditis elegans Genotype: slo-1(gk602291) V. Description: Resistant to the effects of ethanol on egg-laying and locomotion. Derived by outcrossing Million Mutation Project strain VC40372 and verification that this outcrossed strain retains the slo-1(gk602291) allele and resistance to ethanol. Reference: Davis SJ, Scott LL, Hu K & Pierce-Shimomura JT. J Neurosci. 2014 Jul 16;34(29):9562-73. Mutagen: Outcrossed: x6 Made by: Scott Davis Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS470 Species: Caenorhabditis elegans Genotype: vxEx470. Description: vxEx470 [asic-1p::mCherry]. Reporter construct contains 7.5 kb 5' promoter region. Pick animals with red fluorescence in neurons to maintain. Reference: Russell J, et al. Proc Natl Acad Sci USA. 2014 Jun 3;111(22):8269-74. Mutagen: Outcrossed: x0 Made by: Josh Russell Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS471 Species: Caenorhabditis elegans Genotype: asic-1(ok415) I; vxEx283. Description: vxEx283 [mec-10p::asic-1(+) + unc-122p::GFP]. Array rescues asic-1(lf) in ALMl, ALMR, AVM, PLML, PLMR, FLP, and PVD. Pick GFP+ animals to maintain. Reference: Russell J, et al. Proc Natl Acad Sci USA. 2014 Jun 3;111(22):8269-74. Mutagen: Outcrossed: x0 Made by: Josh Russell Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS474 Species: Caenorhabditis elegans Genotype: asic-1(ok415) I; vxEx284. Description: vxEx284 [sto-5p::asic-1(+) + unc-122p::GFP]. Array rescues asic-1(lf) in FLP and BDU neurons. Pick GFP+ animals to maintain. Reference: Russell J, et al. Proc Natl Acad Sci USA. 2014 Jun 3;111(22):8269-74. Mutagen: Outcrossed: x0 Made by: Josh Russell Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS478 Species: Caenorhabditis elegans Genotype: asic-1(ok415) I; mec-10(tm1552) X; vxEx478. Description: vxEx478 [sto-5p::asic-1(+) + unc-122p::GFP]. Array rescues asic-1(lf) in FLP and BDU neurons. Pick GFP+ animals to maintain. Reference: Russell J, et al. Proc Natl Acad Sci USA. 2014 Jun 3;111(22):8269-74. Mutagen: Outcrossed: x0 Made by: Josh Russell Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS48 Species: Caenorhabditis elegans Genotype: vxEx105. Description: vxEx105 [dat-1p::ChR2::mCherry + myo-2p::mCherry]. Raise on retinal for optogenetic manipulations (per Vidal-Gadea, 2011). Not necessary for survival. Reference: Vidal-Gadea A, et al. Proc Natl Acad Sci U S A. 2011 Oct 18;108(42):17504-9. Mutagen: Outcrossed: x0 Made by: Stephen Topper Received: 07/29/16 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS481 Species: Caenorhabditis elegans Genotype: vxEx280. Description: vxEx280 [sto-5p::ICE + gcy-8::ICE + myo-2p::mCherry]. Pick mCherry+ animals to maintain. Genetic ablation of AFDL, AFDR, FLPL, FLPR, BDUL, and BDUR via expression of a human cell death caspase (ICE). Reference: Russell J, et al. Proc Natl Acad Sci USA. 2014 Jun 3;111(22):8269-74. Mutagen: Outcrossed: x0 Made by: Josh Russell Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS482 Species: Caenorhabditis elegans Genotype: tax-4(p678) III; vxEx280. Description: vxEx280 [sto-5p::ICE + gcy-8::ICE + myo-2p::mCherry]. Pick animals with mCherry expression in the pharynx to maintain the array. Genetic ablation of AFD, FLP, and BDU via expression of a human cell death caspase (ICE). Reference: Russell J, et al. Proc Natl Acad Sci USA. 2014 Jun 3;111(22):8269-74. Mutagen: Outcrossed: x0 Made by: Josh Russell Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS540 Species: Caenorhabditis elegans Genotype: tax-4(p678) III; vxEx540. Description: vxEx540 [tax-4(fosmid) + unc-122p::GFP]. Pick animals with GFP expression in coelomocytes to maintain array. This strain rescues tax-4 function with fosmid WRM069cE04 that includes full tax-4 genomic DNA. Reference: Russell J, et al. Proc Natl Acad Sci U S A. 2014 Jun 3;111(22):8269-74. Mutagen: Outcrossed: x0 Made by: Josh Russell Received: 12/15/15 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS569 Species: Caenorhabditis elegans Genotype: che-6(e1126) IV; vxEx280. Description: vxEx280 [sto-5p::ICE + gcy-8::ICE + myo-2p::mCherry]. Pick animals with mCherry expression in the pharynx to maintain the array. Genetic ablation of AFD, FLP, and BDU via expression of a human cell death caspase (ICE). Reference: Russell J, et al. Proc Natl Acad Sci USA. 2014 Jun 3;111(22):8269-74. Mutagen: Outcrossed: x0 Made by: Josh Russell Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS572 Species: Caenorhabditis elegans Genotype: slo-1(js379) V; vsIs48; vxEx345. Description: vsIs48 [unc-17::GFP]. GFP expressed in all cholinergic neurons. vxEx345 [slo-1p::slo-1(+)::mCherry::unc-54 3'UTR + myo-2p::mCherry]. Pick animals with mCherry expression in the pharynx to maintain the array. vxEx345 rescues ethanol-independent and ethanol intoxication phenotypes of a slo-1(null) mutants. vxEx345 expresses worm BK channel protein (slo-1(+)) with a C-terminal mCherry tag. Reference: Davis SJ, Scott LL, Hu K & Pierce-Shimomura JT. J Neurosci. 2014 Jul 16;34(29):9562-73. Mutagen: Outcrossed: x0 Made by: Scott Davis Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS573 Species: Caenorhabditis elegans Genotype: slo-1(js379) V; vsIs48; vxEx348. Description: vsIs48 [unc-17::GFP]. GFP expressed in all cholinergic neurons. vxEx328 [slo-1p::slo-1(T381I)::mCherry::unc-54 3'UTR + myo-2p::mCherry]. Pick animals with mCherry expression in the pharynx to maintain the array. vxEx328 rescues ethanol-independent phenotypes of a slo-1(null) mutants without rescuing ethanol intoxication. vxEx328 expresses worm BK channel protein (slo-1(T381I)) with a C-terminal mCherry tag. Reference: Davis SJ, Scott LL, Hu K & Pierce-Shimomura JT. J Neurosci. 2014 Jul 16;34(29):9562-73. Mutagen: Outcrossed: x0 Made by: Scott Davis Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS574 Species: Caenorhabditis elegans Genotype: slo-1(js379) V; vsIs48; vxEx339. Description: vsIs48 [unc-17::GFP]. GFP expressed in all cholinergic neurons. vxEx339 [slo-1p::hslo(+)::mCherry::unc-54 3'UTR + myo-2p::mCherry]. Pick animals with mCherry expression in the pharynx to maintain the array. vxEx339 rescues ethanol-independent and ethanol intoxication phenotypes of a slo-1(null) mutants. vxEx339 expresses human BK channel protein (hslo(+)) with a C-terminal mCherry tag. Reference: Davis SJ, Scott LL, Hu K & Pierce-Shimomura JT. J Neurosci. 2014 Jul 16;34(29):9562-73. Mutagen: Outcrossed: x0 Made by: Scott Davis Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS575 Species: Caenorhabditis elegans Genotype: slo-1(js379) V; vsIs48; vxEx326. Description: vsIs48 [unc-17::GFP]. GFP expressed in all cholinergic neurons. vxEx326 [slo-1p::hslo(T352I)::mCherry::unc-54 3'UTR + myo-2p::mCherry]. Pick animals with mCherry expression in the pharynx to maintain the array. vxEx326 rescues ethanol-independent phenotypes of a slo-1(null) mutants without rescuing ethanol intoxication. vxEx326 expresses human BK channel protein (hslo(T352I)) with a C-terminal mCherry tag. Reference: Davis SJ, Scott LL, Hu K & Pierce-Shimomura JT. J Neurosci. 2014 Jul 16;34(29):9562-73. Mutagen: Outcrossed: x0 Made by: Scott Davis Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS582 Species: Caenorhabditis elegans Genotype: che-6(e1126) IV; vxEx582. Description: vxEx582 [gcy-8p::ICE + myo-2p::mCherry]. Pick animals with mCherry expression in the pharynx to maintain the array. Genetic ablation of AFD via expression of a human cell death caspase (ICE). Reference: Russell J, et al. Proc Natl Acad Sci USA. 2014 Jun 3;111(22):8269-74. Mutagen: Outcrossed: x0 Made by: Josh Russell Received: 08/14/14 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS611 Species: Caenorhabditis elegans Genotype: vxEx611. Description: vxEx611 [mtq-2p::mCherry::unc-54 UTR + unc-122p::GFP]. Pick mCherry+/GFP+ to maintain. mCherry is expressed in ventral cord neurons and most head neurons. Reference: Nordquist, S.K., Smith, S.R., and Pierce, J.T. (2018) G3: Genes|Genomes|Genetics, g3.200019.2018. http://doi.org/10.1534/g3.118.200019. Mutagen: Outcrossed: x0 Made by: Sarah Nordquist Received: 03/22/18 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS617 Species: C. elegans Genotype: vxIs591. Description: vxIs591 [tph-1p::GFP::unc-54 3'UTR]. GFP expression in all serotonergic neurons. Reference: Sae-Lee et al. G3 (Bethesda) 2020 Aug 5;10(8):2851-2861. PMID: 32580938 Mutagen: Outcrossed: x0 Made by: Luisa Scott Received: 10/07/20 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS809 Species: C. elegans Genotype: vxSi808 X; vxIs591. Description: vxSi808 [rab-3p::APP::mCherry::unc-54 3'UTR] X. vxIs591 [tph-1p::GFP::unc-54 3'UTR]. Single-copy insertion of human APP transgene tagged with mCherry expressed throughout the nervous system, visible along ventral nerve cord, head, tail, and other neurons. GFP expression in all serotonergic neurons. References: Yi B, et al. 2017 J Neurochem 140(4): 561-575. PMID: 27926996. Mondal et al. 2018 ACS Chem Neurosci. DOI: 10.1021/acschemneuro.7b00428 PMID: 29426225 Mutagen: MosSCI Outcrossed: x1 Made by: Wisath Sae-Lee Received: 10/07/20 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS844 Species: C. elegans Genotype: vxIs824; vxIs591. Description: vxIs824 [rab-3p::ND18ApoE4::unc-54 3'UTR + myo-2p::mCherry::unc-54 3'UTR]. vxIs591 [tph-1p::GFP::unc-54 3'UTR]. Integrated transgene driving expression of human APOE4 throughout the nervous system; induces age-related neurodegeneration of HSNs and bag-of-worms in up to 60% of D3 adult worms. GFP expression in all serotonergic neurons. Reference: Sae-Lee et al. G3 (Bethesda) 2020 Aug 5;10(8):2851-2861. PMID: 32580938 Mutagen: Outcrossed: x1 Made by: Wisath Sae-Lee Received: 10/07/20 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JPS845 Species: C. elegans Genotype: vxIs823 II; vxIs824; vxIs591. Description: vxIs823 [rab-3p::APP::mCherry::unc-54 3'UTR] II. vxIs824 [rab-3p::ND18ApoE4::unc-54 3'UTR + myo-2p::mCherry::unc-54 3'UTR]. vxIs591 [tph-1p::GFP::unc-54 3'UTR]. Integrated transgenes driving expression of human APOE4 and human APP throughout the nervous system; induces age-related neurodegeneration of HSNs and bag-of-worms in >60% of D3 adult worms. GFP expression in all serotonergic neurons. Reference: Sae-Lee et al. G3 (Bethesda) 2020 Aug 5;10(8):2851-2861. PMID: 32580938 Mutagen: Outcrossed: x1 Made by: Susan Rozmiarek Received: 10/07/20 University of Texas at Austin, Austin TX, USA ----------------------------------------------------------------------------- Strain: JR1000 Species: Caenorhabditis elegans Genotype: ced-1(e1735) I; unc-119(ed4) III; wIs78 IV. Description: wIs78 [SCMp::GFP + ajm-1p::GFP + F58E10 (cosmid) + unc-119(+)] IV. GFP expression in seam cells and adherens junctions. ajm-1 previously called jam-1. Mutagen: Gamma Rays Outcrossed: x0 Made by: Morris Maduro Received: 11/10/01 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: JR113 Species: Caenorhabditis elegans Genotype: sma-1(e30) unc-76(e911) wDf2/sqt-3(sc8) unc-61(e228) V. Description: Heterozygotes are WT and segregate WT, RolUncs and dead eggs. Homozygous wDf1 embryos arrest uniformly as unenclosed balls of differentiated cells. wDf2 formerly called zen-1(w1). sc8 previously called rol-4(sc8). Mutagen: EMS Outcrossed: x4 Made by: Paul Heid Received: 07/14/97 University of California, Santa Barbara CA, USA ----------------------------------------------------------------------------- Strain: JR1279 Species: Caenorhabditis elegans Genotype: cep-1(+) I; cep-1(w40). Description: Resistant to germ cell death induced by ionizing radiation. Sensitive to hypoxia and starvation stresses. This strain contains an intact copy of the gene at the normal locus on LG I. w40 is a translocated partially deleted copy of the cep-1 locus that has a dominant-negative effect on cep-1 function and is not linked to the chromosomal location of cep-1. Mutagen: UV/TMP Outcrossed: x10 Made by: W. B. Derry Received: 02/12/02 University of California, Santa Barbara CA, USA ----------------------------------------------------------------------------- Strain: JR1380 Species: Caenorhabditis elegans Genotype: die-1(w34)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are wild type and segregate wild type, paralyzed Dpys, and die-1 homozygous embryos. Mutagen: EMS Outcrossed: x Made by: Steve Gendreau Received: 02/12/02 University of California, Santa Barbara CA, USA ----------------------------------------------------------------------------- Strain: JR1763 Species: Caenorhabditis elegans Genotype: wcDf1 dpy-1(e1)/daf-7(e1372) par-2(it46) III. Description: At 25C, heterozygotes are WT and segregate WT, dead eggs and Dauers (which will give only dead eggs if they exit dauer). e1372 and it46 are both temperature sensitive. Mutagen: Outcrossed: x Made by: Received: 03/20/00 University of California, Santa Barbara CA, USA ----------------------------------------------------------------------------- Strain: JR1904 Species: Caenorhabditis elegans Genotype: wIs117. Description: wIs117 [med-1p::GFP::pop-1::med-1 3'UTR + rol-6(su1006)]. Rollers. Reference: Maduro MF, Lin R & Rothman JH. 2002. Developmental Biology 248: 128-142. Mutagen: Gamma radiation Outcrossed: x0 Made by: Morris Maduro Received: 10/01/15 University of California, Riverside, CA ----------------------------------------------------------------------------- Strain: JR2274 Species: Caenorhabditis elegans Genotype: wIs137 V. Description: wIs137 [end-3p::end-3(P202L)::GFP + rol-6(su1006)] V. Rollers. Reference: Maduro MF, et al. Dev Biol. 2005 Aug 15;284(2):509-22. Mutagen: Gamma radiation Outcrossed: x0 Made by: Morros Maduro Received: 09/22/14 University of California, Riverside, CA ----------------------------------------------------------------------------- Strain: JR2370 Species: Caenorhabditis elegans Genotype: egl-18(ok290) IV. Description: Mutagen: UV/TMP Outcrossed: x4 Made by: Received: 03/25/05 University of California, Santa Barbara CA, USA ----------------------------------------------------------------------------- Strain: JR2750 Species: Caenorhabditis elegans Genotype: bli-6(sc16) unc-22(e66)/unc-24(e138) vha-17(w13) dpy-20(e2017) IV. Description: Pick wild type animals to maintain. Mutagen: Outcrossed: x4 Made by: Kenji Kontani Received: 02/08/06 University of California, Santa Barbara CA, USA ----------------------------------------------------------------------------- Strain: JR36 Species: Caenorhabditis elegans Genotype: cdl-1(e2510)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, dead eggs (homozygous cdl-1(e2510), and Dpy Unc. e2510 carries a missense mutation at the 249th amino acid from Pro to Ser. Mutagen: EMS Outcrossed: x Made by: Joel Rothman Received: 05/22/02 Tohoku University, Sendai, Japan ----------------------------------------------------------------------------- Strain: JR41 Species: Caenorhabditis elegans Genotype: unc-76(e911) wDf1/unc-61(e228) dpy-21(e428) V. Description: Heterozygotes are WT and segregate WT, DpyUncs and dead eggs. Homozygous wDf1 embryos arrest uniformly as unenclosed balls of differentiated cells. wDf1 formerly called zen-1(e2482). 2/02: dpy-21 appears to have been lost from this strain. Mutagen: EMS Outcrossed: x>2 Made by: Joel Rothman Received: 07/14/97 University of California, Santa Barbara CA, USA ----------------------------------------------------------------------------- Strain: JR417 Species: Caenorhabditis elegans Genotype: wDf3/dpy-? V. Description: Pick WT to maintain. This strain is balanced by a dumpy, but not sure which one. wDf3 arrests as dead eggs with no gut. Mutagen: EMS Outcrossed: x>2 Made by: Steve Gendreau Received: 12/01/98 University of California, Santa Barbara CA, USA ----------------------------------------------------------------------------- Strain: JR423 Species: Caenorhabditis elegans Genotype: rhDf1/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, Dpy Steriles and dead eggs. Mutagen: Gamma Rays Outcrossed: x Made by: Asako Sugimoto Received: 03/26/96 University of California, Santa Barbara CA, USA ----------------------------------------------------------------------------- Strain: JR667 Species: Caenorhabditis elegans Genotype: unc-119(e2498::Tc1) III; wIs51 V. Description: wIs51 [SCMp::GFP + unc-119(+)] V. GFP expression in seam cells. Superficially wild-type. Mutagen: Outcrossed: x1 Made by: Jodie Kasmir Received: 08/28/00 University of California, Santa Barbara CA, USA ----------------------------------------------------------------------------- Strain: JR728 Species: Caenorhabditis elegans Genotype: sqt-3(sc8) unc-61(e228)/wDf4 V. Description: Pick WT to maintain. Throws WT, Roller Uncs and dead eggs. wDf4 arrests as dead eggs with no gut. sc8 previously called rol-4(sc8). Mutagen: EMS Outcrossed: x>2 Made by: Steve Gendreau Received: 12/01/98 University of California, Santa Barbara CA, USA ----------------------------------------------------------------------------- Strain: JR8 Species: Caenorhabditis elegans Genotype: die-1(e2500)/mnC1 [dpy-10(e128) unc-52(e444)] II; him-8(e1489) IV. Description: Heterozygotes are WT and segregate WT, paralyzed Dpys, and arrested embryos. Throws males. e2500 pka zen-2(e2500). Mutagen: EMS Outcrossed: x5 Made by: Joel Rothman Received: 02/12/02 University of California, Santa Barbara CA, USA ----------------------------------------------------------------------------- Strain: JRB1 Species: Halicephalobus mephisto Genotype: Halicephalobus mephisto wild isolate. Description: Halicephalobus mephisto wild isolate. Grow at 37C: can survive higher temperatures than C. elegans. Useful model organism for studying heat tolerance; has expanded Hsp70 and AIG1 gene families. Has approximately 1.15% snp heterozygosity. Parthenogenetic reproduction so it cannot be out-crossed. References: Borgonie G, et al. Nature. 2011 Jun 2;474(7349):79-82. Weinstein DJ, et al. Nat Commun. 2019 Nov 21;10(1):5268. Mutagen: Outcrossed: x0 Made by: John Bracht Received: 09/23/20 American University ----------------------------------------------------------------------------- Strain: JS345 Species: Caenorhabditis elegans Genotype: gck-1(km15) V/nT1 [qIs51] (IV;V). Description: Maintain by picking GFP+ wild-type worms. Heterozygotes segregate wild-type GFP+ heterozygotes, sterile GFP- gck-1 homozygotes, and dead eggs (nT1 homozygotes). Reference: Schouest et al, Plos ONE 4(10):e7450 (2009). Mutagen: Outcrossed: x Made by: Katie Schouest Received: 11/06/09 University of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: JS604 Species: Caenorhabditis elegans Genotype: dpy-17(e164) tlk-1(tm2395) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and Dpy Stu homozygotes that are non-GFP. Homozygous hT2[bli-4 let-? qIs48] inviable. Mutagen: Outcrossed: x2 Made by: Tokiko Furuta Received: 11/14/07 University of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: JS71 Species: Caenorhabditis elegans Genotype: dpy-11(e224) air-1(vw5) V/eT1 (III;V). Description: Heterozygotes are WT and segregate WT, Dpy Stu, Unc-36 (eT1 homozygotes) and dead eggs. Also weak Him. Mutagen: UV/TMP Outcrossed: x>7 Made by: Tokiko Furuta Received: 04/06/04 University of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: JS803 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; vwIs346. Description: vwIs346 [pie-1p::LAP::CDC-48.2 + unc-119(+)]. Mutagen: Outcrossed: x Made by: Tokiko Furuta Received: 02/26/09 University of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: JS83 Species: Caenorhabditis elegans Genotype: air-1(vw5) V/eT1 (III;V). Description: Heterozygotes are WT and segregate WT, Unc-36, Steriles (vw5 homozygotes) and dead eggs. Mutagen: TMP Outcrossed: x3 Made by: Tokiko Furuta Received: 08/30/04 University of Texas, Houston, TX ----------------------------------------------------------------------------- Strain: JT10066 Species: Caenorhabditis elegans Genotype: unc-1(e719) pdk-1(sa680) X. Description: Unc. Daf-c at 25C. Egl, Clumpy, Lon, low brood size. Dauers do not recover when moved to 15C in the presence of food. Daf-c, Lon, Clumpy and fertility defects can be rescued maternally. Maintain at 15C. Mutagen: Outcrossed: x Made by: Michael Ailion Received: 08/27/99 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT10189 Species: Caenorhabditis elegans Genotype: daf-14(m77) IV; scd-2(sa935) V. Description: Daf-c strongly but not completely suppressed; some dauers visible at 25C. sa935 alone is moderate Daf-d (dauer defective) in plate starvation assays, and resistant to exogenous dauer pheromone. sa935 confers strong suppression of daf-8 and daf-14, weak suppression of daf-1, -4, -7. scd-2(sa935) single mutant looks WT. snb-1(md247) hypomorph is an excellent balancer for scd-2; snb-1 is immediately neighboring. Maintain at 15 degrees. Mutagen: EMS Outcrossed: x2 Made by: Takao Inoue Received: 11/24/09 University of California-Berkeley, CA, USA ----------------------------------------------------------------------------- Strain: JT1058 Species: Caenorhabditis elegans Genotype: hid-1(sa1058) X. Description: Hid. Aex. Dec-s. Unc. Mutagen: EMS Outcrossed: x2 Made by: Michael Ailion Received: 10/15/04 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT10783 Species: Caenorhabditis elegans Genotype: scd-2(sa935) V; lin-15B&lin-15A(n765) X; saEx472. Description: saEx472 [(pTJ1369) scd-2(+) + lin-15(+)]. Pick wildtype worms, maintain at 25 degrees (non-transgenics will be Muv). Mutagen: Outcrossed: x Made by: Michael Ailion Received: 11/24/09 University of California-Berkeley, CA, USA ----------------------------------------------------------------------------- Strain: JT10800 Species: Caenorhabditis elegans Genotype: ncr-2(nr2023) III; ncr-1(nr2022) X. Description: Daf-c (partial dauer), vulval abnormalities, broken alae, gonadal migration defects, low brood size, short lifespan. May grow better at 15C. Strain is healthier when recovered from starved plates by chunking than when continuously propagated. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: EMS Outcrossed: x>8 Made by: Jie Li Received: 09/11/06 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT11069 Species: Caenorhabditis elegans Genotype: xbx-1(ok279) V. Description: Dyf. Osm. Reduced mating efficiency (ME 2-3). Deletion extends over 1610 bp in the intron between exons 3 and 4 and ending 30 bp after the STOP codon (cosmid F02D8 pb 25954-27563 are deleted). Complements dyf-4(m158). Mutagen: UV/TMP Outcrossed: x7 Made by: OMRF Knockout Group Received: 01/27/03 Karolinska Institute, Huddinge, Sweden ----------------------------------------------------------------------------- Strain: JT11323 Species: Caenorhabditis elegans Genotype: heh-1(ok603) III. Description: Cholesterol concentration-dependent Daf-c. If cholesterol is omitted from NGM, animals form dauers (no dauers are formed on regular NGM). Temperature sensitive Daf-c; heh-1 animals more readily form dauers at 27C than WT animals. Partial dauer phenotype; dauers exhibit ale but do not fully constrict pharynges nor arrest germline proliferation. On low cholesterol, vulva protrudes and sometimes ruptures; animals exhibit egg-laying defects. Hypersensititve to progesterone in culture media. Mutagen: UV/TMP Outcrossed: x3 Made by: Gemma Brown Received: 09/05/07 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT11398 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Reference: Andersen EC, et al. Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: J. Kemner Received: 09/04/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: JT156 Species: Caenorhabditis elegans Genotype: daf-12(sa156) X. Description: daf-d. Weak heterochronic phenotypes in seam, somatic gonad, intestine. Class III allele. Mutagen: Outcrossed: x Made by: Jim Thomas Received: 10/23/00 Max Planck Institute, Cologne, Germany ----------------------------------------------------------------------------- Strain: JT191 Species: Caenorhabditis elegans Genotype: daf-28(sa191) V. Description: Temperature sensitive dauer constitutive. Highly penetrant at 25C, much less so at 20C and 15C. The dauers recover almost immediately after temperature down shift, so one may work with synchronous broods. Semi-dominant: about 10% of heterozygotes form dauers. Mutagen: EMS Outcrossed: x2 Made by: Betsy Malone Received: 11/15/93 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT193 Species: Caenorhabditis elegans Genotype: daf-2(sa193) III. Description: Adults Age and Itt, but not Unc (class 1 allele). sa193 results in an amino acid substitution(A580T) in the extracellular portion of the DAF-2 receptor (specifically, the L2 domain). Mutagen: EMS Outcrossed: x Made by: Betsy Malone Received: 10/09/08 University College, London, England ----------------------------------------------------------------------------- Strain: JT200 Species: Caenorhabditis elegans Genotype: unc-43(sa200) IV. Description: Defecation motor program executed twice each cycle period. Hyperactive loopy movement. Previously called dec-8(sa200). Mutagen: EMS Outcrossed: x4 Made by: Dennis Liu Received: 07/18/94 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT204 Species: Caenorhabditis elegans Genotype: daf-12(sa204) X. Description: Lon. Daf-d. Mutagen: EMS Outcrossed: x2 Made by: Jim Thomas Received: 10/17/05 Karolinska Institute, Huddinge, Sweden ----------------------------------------------------------------------------- Strain: JT23 Species: Caenorhabditis elegans Genotype: aex-5(sa23) I. Description: aBoc and Exp defective. Constipated. Mutagen: EMS Outcrossed: x2 Made by: Jim Thomas Received: 01/25/93 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT24 Species: Caenorhabditis elegans Genotype: aex-6(sa24) I. Description: aBoc and Exp defective. Constipated. Mutagen: EMS Outcrossed: x2 Made by: Jim Thomas Received: 01/25/93 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT248 Species: Caenorhabditis elegans Genotype: scd-1(sa248) X. Description: Mutagen: EMS Outcrossed: x2 Made by: Takao Inoue Received: 03/01/01 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT249 Species: Caenorhabditis elegans Genotype: scd-2(sa249) V. Description: Responds poorly to dauer pheromone. Mutagen: EMS Outcrossed: x2 Made by: Takao Inoue Received: 03/01/01 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT253 Species: Caenorhabditis elegans Genotype: scd-3(sa253) I. Description: Responds poorly to dauer pheromone. Low brood size. Egl. Gonadal left/right reversal. Mab. Mutagen: EMS Outcrossed: x2 Made by: Takao Inoue Received: 03/01/01 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT292 Species: Caenorhabditis elegans Genotype: dec-11(sa292) IV. Description: Long defecation cycle period. Slightly slow growing and scrawny. Mutagen: EMS Outcrossed: x2 Made by: K. Iwasaki Received: 02/14/96 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT293 Species: Caenorhabditis elegans Genotype: dec-9(sa293) IV. Description: Short defecation cycle period. Semi-dominant. Mutagen: EMS Outcrossed: x2 Made by: K. Iwasaki Received: 02/14/96 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT294 Species: Caenorhabditis elegans Genotype: dec-10(sa294) X. Description: Short defecation cycle period. Dominant. Mutagen: EMS Outcrossed: x2 Made by: K. Iwasaki Received: 02/14/96 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT295 Species: Caenorhabditis elegans Genotype: dec-12(sa295) I. Description: Long defecation cycle period. Slightly slow growing and scrawny. Mutagen: EMS Outcrossed: x2 Made by: Iwasaki/Liu/Thomas Received: 02/14/96 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT296 Species: Caenorhabditis elegans Genotype: dec-7(sa296) III. Description: Temperature sensitive. Short defecation cycle period at 20C, longer at 25C. Appears to feed normally. Mutagen: EMS Outcrossed: x2 Made by: Kouichi Iwasaki Received: 03/12/96 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT3 Species: Caenorhabditis elegans Genotype: aex-2(sa3) X. Description: Constipated. aBoc and Exp defective. Mutagen: EMS Outcrossed: x2 Made by: Jim Thomas Received: 01/15/93 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT307 Species: Caenorhabditis elegans Genotype: egl-9(sa307) V. Description: 243 bp internal deletion. Mutagen: EMS Outcrossed: x5 Made by: C Darby Received: 10/09/01 University of California, San Francisco, CA ----------------------------------------------------------------------------- Strain: JT363 Species: Caenorhabditis elegans Genotype: nrf-3(sa363) IV. Description: Nrf. Mutagen: EMS Outcrossed: x2 Made by: Robert Choy Received: 01/21/00 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT366 Species: Caenorhabditis elegans Genotype: vhp-1(sa366) II. Description: Nrf. sa366 previously called nrf-2. Mutagen: EMS Outcrossed: x2 Made by: Robert Choy Received: 01/21/00 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT37 Species: Caenorhabditis elegans Genotype: lag-2(sa37) V. Description: Semidominant suppressor of lin-12(d) alleles. Mutagen: EMS Outcrossed: x Made by: F. Tax Received: 07/18/94 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT47 Species: Caenorhabditis elegans Genotype: egl-8(sa47) V. Description: Mutagen: Outcrossed: x Made by: J Thomas Received: 12/01/10 California Institute of Technology, Pasadena, CA ----------------------------------------------------------------------------- Strain: JT48 Species: Caenorhabditis elegans Genotype: dec-1(sa48) X. Description: Constipated. Long defecation cycle in mid to late adults. Mutagen: EMS Outcrossed: x2 Made by: Jim Thomas Received: 01/15/93 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT5 Species: Caenorhabditis elegans Genotype: aex-3(sa5) X. Description: Constipated. aBoc and Exp defective. Mutagen: EMS Outcrossed: x2 Made by: Jim Thomas Received: 01/15/93 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT513 Species: Caenorhabditis elegans Genotype: nrf-5(sa513) V. Description: Nrf, Peg, accumulates yolk in pseudo-coelomic space, slow growing, partial embryonic lethality. Mutagen: EMS Outcrossed: x2 Made by: Robert Choy Received: 01/21/00 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT5132 Species: Caenorhabditis elegans Genotype: +/eT1 III; exp-2(sa26)/eT1 [let-?(n886)] V. Description: Heterozygotes have jerky movement, are Exp defective, and are Egl (dominant). Homozygous exp-2 are recessive lethal. Homozygous eT1 are lethal also. Mutagen: EMS Outcrossed: x2 Made by: Jim Thomas Received: 01/15/93 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT5205 Species: Caenorhabditis elegans Genotype: lin-12(n950) III; lag-2(sa37) V. Description: lag-2(sa37) is a semi-dominant suppressor of lin-12(n302sd). Mutagen: EMS Outcrossed: x Made by: Received: 10/08/99 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT5210 Species: Caenorhabditis elegans Genotype: lin-12(n302) III; emb-4(sa44) V. Description: emb-4(sa44) is a recessive suppressor of lin-12(n302sd). Mutagen: EMS Outcrossed: x Made by: Received: 10/08/99 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT5218 Species: Caenorhabditis elegans Genotype: sup-17(sa38) I; lin-12(n302) III. Description: sa38 is a weak Egl and a moderate suppressor of lin-12(gf). Mutagen: EMS Outcrossed: x Made by: F. Tax / J. Thomas Received: 03/25/05 MIT, Cambridge MA, USA ----------------------------------------------------------------------------- Strain: JT5222 Species: Caenorhabditis elegans Genotype: sup-19(sa45) I; lin-12(n302) III. Description: sa45 is a weak Egl and a moderate suppressor of lin-12(gf). Mutagen: EMS Outcrossed: x Made by: F. Tax / J. Thomas Received: 03/25/05 MIT, Cambridge MA, USA ----------------------------------------------------------------------------- Strain: JT524 Species: Caenorhabditis elegans Genotype: nrf-1(sa524) III. Description: Moderate Nrf, gonad migration defective. Mutagen: EMS Outcrossed: x2 Made by: Robert Choy Received: 01/21/00 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT5244 Species: Caenorhabditis elegans Genotype: aex-4(sa22) X. Description: Constipated. aBoc and Exp defective. Mutagen: EMS Outcrossed: x3 Made by: Jim Thomas Received: 01/15/93 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT525 Species: Caenorhabditis elegans Genotype: nrf-6(sa525) II. Description: Nrf, Peg, accumulates yolk in pseudo-coelomic space, slow growing, partial embryonic lethality. Mutagen: EMS Outcrossed: x2 Made by: Robert Choy Received: 01/21/00 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT5277 Species: Caenorhabditis elegans Genotype: sel-8(sa54) lin-12(n302) III. Description: sel-8(sa54) is a recessive suppressor of lin-12(n302sd). Mutagen: EMS Outcrossed: x Made by: Received: 10/08/99 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT528 Species: Caenorhabditis elegans Genotype: nrf-4(sa528) I. Description: Moderate Nrf. Produces pale eggs (Peg) but does not accumulate yolk in pseudo-coelomic space. Mutagen: EMS Outcrossed: x2 Made by: Robert Choy Received: 01/21/00 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT5283 Species: Caenorhabditis elegans Genotype: lin-12(n302) III; sel-7(n1253) X. Description: sel-7(n1253) is a recessive suppressor of lin-12(n302sd). Mutagen: EMS Outcrossed: x Made by: Received: 10/08/99 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT529 Species: Caenorhabditis elegans Genotype: ndg-4(sa529) III. Description: Ndg: nordihydroguairetic acid resistant. Nrf: nose resistant to fluoxetine-induced hypercontraction. Pale eggs. Accumulates yolk. Some embryonic lethality. Nonsense mutation W404stop(UGA). Mutagen: EMS Outcrossed: x Made by: RKM Choy Received: 08/15/06 Oxford University, Oxford, England ----------------------------------------------------------------------------- Strain: JT5516 Species: Caenorhabditis elegans Genotype: saDf1/lon-1(e185) III. Description: Heterozygotes are WT and segregate WT, Lon and dead eggs. Hets are slow growing and sick. Mutagen: X-rays Outcrossed: x>2 Made by: Jim Thomas Received: 11/18/93 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT573 Species: Caenorhabditis elegans Genotype: akt-1(sa573) V. Description: Hid. Bor. Mutagen: EMS Outcrossed: x2 Made by: Michael Ailion Received: 09/16/04 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT6 Species: Caenorhabditis elegans Genotype: exp-1(sa6) II. Description: Exp defective. Constipated. May contain a weak daf-2 mutation (sa753). See Ailion and Thomas in Genetics 165: 127-144 2003. Mutagen: EMS Outcrossed: x2 Made by: Jim Thomas Received: 01/25/93 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT603 Species: Caenorhabditis elegans Genotype: gpb-2(sa603) I. Description: Recessive, loss of function, early stop mutation within the coding sequence makes sa603 a likely null allele. Variable locomotion ranging from lethargic to hyperactive. Eat (pale, scrawny). Loopy movement - increased amplitude of locomotory wave-form (variable). Suppresses the enteric muscle contraction (EMC) defect, the lethargy and egg-laying defects of unc-43(n498). Mutagen: EMS Outcrossed: x2 Made by: Reiner/Knapp Received: 03/01/01 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT609 Species: Caenorhabditis elegans Genotype: eat-16(sa609) I. Description: Recessive. Loss of function. Hyperactive, lays early stage eggs. Suppresses the enteric muscle contraction (EMC) defect, the lethargy and the egg-laying defects of unc-43(n498). Mutagen: EMS Outcrossed: x3 Made by: Reiner/Knapp Received: 03/01/01 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT6130 Species: Caenorhabditis elegans Genotype: hsp-90(p673) V. Description: Recessive Daf-c mutation that also causes Odr and Che defects. Maintain at 15C. [The mec-1 mutation present in the original isolates has been eliminated.] Previously known as daf-21. Mutagen: Outcrossed: x6 Made by: Received: 02/03/95 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT6428 Species: Caenorhabditis elegans Genotype: exp-3(n2372) V. Description: Defective in enteric muscle contraction (expulsion) step of defecation motor program and egg-laying (severe bloating-Egl Type A: serotonin and imipramine resistant). Enteric and egg-laying muscle structures are normal. Semi-dominant for Egl defect, but not for constipation defect. n2372/+ : ME3. ES3: adult heterozygotes and homozygotes. Mutagen: EMS Outcrossed: x2 Made by: E. Jorgensen Received: 02/14/96 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT646 Species: Caenorhabditis elegans Genotype: hid-3(sa646) I. Description: Hid. Insensitive to dauer-inducing pheromone. Mutagen: EMS Outcrossed: x2 Made by: Michael Ailion Received: 10/15/04 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT666 Species: Caenorhabditis elegans Genotype: hid-4(sa666) IV. Description: Hid. Mutagen: EMS Outcrossed: x2 Made by: Michael Ailion Received: 10/15/04 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT6924 Species: Caenorhabditis elegans Genotype: daf-19(m86) II; daf-12(sa204) X. Description: Lon. Daf-d. Suppresses Daf-c phenotype of daf-19(m86). Dyf. Mutagen: EMS Outcrossed: x2 Made by: Jim Thomas Received: 10/17/05 Karolinska Institute, Huddinge, Sweden ----------------------------------------------------------------------------- Strain: JT6932 Species: Caenorhabditis elegans Genotype: exp-4(n2373) IV. Description: Defective in enteric muscle contraction (expulsion) step of defecation motor program and egg-laying (severe bloating- Egl Type A). Enteric and egg-laying muscle birefringence normal. Semi-dominant: n2373/+ is moderately Egl but not constipated. ME3 : n2373/+. ES3: adult homozygotes. ES2: heterozygotes. Mutagen: EMS Outcrossed: x4 Made by: E. Jorgensen Received: 02/14/96 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT698 Species: Caenorhabditis elegans Genotype: hid-2(sa698) X. Description: Hid. Mutagen: EMS Outcrossed: x2 Made by: Michael Ailion Received: 10/15/04 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT699 Species: Caenorhabditis elegans Genotype: aex-6(sa699) I. Description: Hid. Con. Egl. Lethargic. Moderately Ric. Mutagen: EMS Outcrossed: x2 Made by: Michael Ailion Received: 09/16/04 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT7 Species: Caenorhabditis elegans Genotype: pbo-1(sa7) III. Description: Constipated. pBoc weak. Very slow growing. Mutagen: EMS Outcrossed: x2 Made by: Jim Thomas Received: 01/15/93 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT709 Species: Caenorhabditis elegans Genotype: pdk-1(sa709) X. Description: Daf-c at 27C. Weakly Egl and Clumpy. Maintain at 15C. Mutagen: EMS Outcrossed: x2 Made by: Michael Ailion Received: 08/27/99 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT711 Species: Caenorhabditis elegans Genotype: hid-6(sa711) I. Description: Hid. Mutagen: EMS Outcrossed: x2 Made by: Michael Ailion Received: 09/16/04 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT722 Species: Caenorhabditis elegans Genotype: hid-1(sa722) X. Description: Hid. Aex. Dec-S. Unc. Mutagen: EMS Outcrossed: x2 Made by: Michael Ailion Received: 09/16/04 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT725 Species: Caenorhabditis elegans Genotype: hid-5(sa725) X. Description: Hid. Egl. Weakly Clumpy. Mutagen: EMS Outcrossed: x2 Made by: Michael Ailion Received: 09/16/04 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT73 Species: Caenorhabditis elegans Genotype: itr-1(sa73) IV. Description: Long defecation cycle period. Slightly slow growing and scrawny. Reduced brood size, especially at 25C. See also WBPaper00003693; previously called dec-4(sa73). Mutagen: EMS Outcrossed: x2 Made by: P. Liu Received: 02/14/96 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT734 Species: Caenorhabditis elegans Genotype: goa-1(sa734) I. Description: Recessive, early stop mutation within the coding sequence (C to T substitution in aa52) makes sa734 a likely null allele. May grow slightly better at 15C. Hyperactive, lays early stage eggs, increased amplitude of locomotort wave-form. Suppresses the lethargy and egg-laying defects of unc-43(n498). Reverses direction of locomotion more frequently than WT. Mutagen: EMS Outcrossed: x3 Made by: Merrilee Robatzek Received: 03/01/01 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT748 Species: Caenorhabditis elegans Genotype: dgk-1(sa748) X. Description: Recessive. Loss of function. Hyperactive, lays early stage eggs. Suppresses both the lethargy and egg-laying defect of unc-43(n498). Mutagen: EMS Outcrossed: x2 Made by: Merrilee Robatzek Received: 03/01/01 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT8132 Species: Caenorhabditis elegans Genotype: lin-49(sa470) IV; him-5(e1490) V. Description: Males are abnormal. Variable additional defects: Con, Egl, Unc. Temperature sensitive. See also WBPaper00003938. Mutagen: EMS Outcrossed: x Made by: Helen Chamberlin Received: 02/28/00 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT8193 Species: Caenorhabditis elegans Genotype: lin-59(sa489) I; him-5(e1490) V. Description: Males are abnormal. Variable additional defects: Con, Egl, Unc. Temperature sensitive. See also WBPaper00003938. Mutagen: EMS Outcrossed: x Made by: Helen Chamberlin Received: 02/28/00 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT8724 Species: Caenorhabditis elegans Genotype: hid-7(sa572) IV. Description: Hid. Mutagen: EMS Outcrossed: x0 Made by: Michael Ailion Received: 09/16/04 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT89 Species: Caenorhabditis elegans Genotype: dec-2(sa89) III. Description: Long defecation cycle period. Slightly slow growing and scrawny. Mutagen: Background mutation in CB1338 Outcrossed: x2 Made by: P. Liu/J.H. Thomas Received: 02/14/96 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT9 Species: Caenorhabditis elegans Genotype: aex-1(sa9) I. Description: Constipated. aBoc and Exp defective. Mutagen: EMS Outcrossed: x2 Made by: Jim Thomas Received: 01/15/93 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT94 Species: Caenorhabditis elegans Genotype: unc-25(sa94) III. Description: Temperature sensitive. Weak allele. Mutagen: EMS Outcrossed: x2 Made by: Yishi Jin Received: 09/17/99 MIT, Cambridge MA, USA ----------------------------------------------------------------------------- Strain: JT9461 Species: Caenorhabditis elegans Genotype: lin-48(sa469) III; him-5(e1490) V. Description: Male spicule defect. Mutagen: EMS Outcrossed: x2 Made by: H. Chamberlin Received: 09/07/01 Ohio State University, Columbus, OH ----------------------------------------------------------------------------- Strain: JT9609 Species: Caenorhabditis elegans Genotype: pdk-1(sa680) X. Description: Daf-c at 25C. Egl, Clumpy, Lon, low brood size. Dauers do not recover when moved to 15C in the presence of food. Daf-c, Lon, Clumpy and fertility defects can be rescued maternally. Maintain at 15C. Mutagen: EMS Outcrossed: x4 Made by: Michael Ailion Received: 08/27/99 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JT9819 Species: Caenorhabditis elegans Genotype: unc-24(e138) lin-49(s1198) unc-22(s7) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Unc, lethal Twitchers and dead eggs. Twitchers arrest in early-mid larval development. See also WBPaper00003938. Mutagen: Outcrossed: x Made by: Received: 02/28/00 University of Washington, Seattle ----------------------------------------------------------------------------- Strain: JTL611 Species: C. elegans Genotype: hsf-1(ljt3[hsf-1::degron::gfp]) I; ieSi57 II; unc-119(ed3) III. Description: ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR + Cbr-unc-119(+)] II. Endogenous hsf-1 tagged with the auxin-inducible-degron and GFP allows depletion of endogenous HSF-1 in the somatic tissues upon auxin treatment. Animals treated with 1mM of auxin when eggs are laid will arrest in L1 or L2 stage. Reference: Edwards SL, et al. Cell Rep. 2021 Aug 31;36(9):109623. PMID2021 Aug 31;36(9):109623. PMID: 34469721 Mutagen: Outcrossed: x6 Made by: Alison Jalanti Received: 09/22/21 New York Medical College ----------------------------------------------------------------------------- Strain: JTL720 Species: C elegans Genotype: haps-1(ljt1) I. Description: CRISPR-engineered KO strain of haps-1 made by knock-in of tandem stop codons to the first exon of endogenous haps-1 locus. haps-1(C48B6.3) is the C. elegans ortholog of human HAPSTR1. haps-1(ljt1) is superficially wild-type when cultured at 20C, but is hypersensitive to paraquat (redox stress), MLN4924 (neddylation inhibition), and camptothecin (genotoxic stress). Reference: Amici DR, et al. Proc Natl Acad Sci U S A. 2022 Jul 5;119(27):e2111262119. doi: 10.1073/pnas.2111262119. PMID: 35776542. Mutagen: Outcrossed: x6 Made by: Jian Li Received: 05/30/24 New York Medical College ----------------------------------------------------------------------------- Strain: JU1018 Species: Caenorhabditis briggsae Genotype: mfIs42. Description: mfIs42 [Cel-sid-2 + Cel-myo-2::DsRed]. JU977 integrated with gamma-rays. Mutagen: Outcrossed: x5 Made by: Received: 03/01/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1038 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: Isolated from rotting pears sampled below their tree on 15 Oct 2006 in Le Blanc (Indre, France). Sample plated on 16 Oct 2006. Picked as an adult on 18 Oct 2006. PrA1. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 03/01/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1082 Species: Caenorhabditis remanei Genotype: C. remanei wild isolate. Description: Male-female strain. Isolated by Marie-Anne Felix from decomposing fruit and vegetables sampled on March 11, 2007, in small vegetable gardens in Okazaki (East of Nagoya), Aichi Prefecture, Japan. Isofemale line. Maintain by mating. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 09/24/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1084 Species: Caenorhabditis remanei Genotype: C. remanei wild isolate. Description: Male-female strain. Isolated by Marie-Anne Felix from decomposing fruit and vegetables sampled on March 14, 2007, in small vegetable gardens next to the Kacho-en aviary (ca. 100 m) in Kakegawa, Shizuoka prefecture, Japan. Isofemale line. Maintain by mating. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 09/24/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1085 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: Isolated by Marie-Anne Felix from leaf litter/bark/soil sampled on March 14, 2007, in the woods behind the parking lot of the Kacho-en aviary in Kakegawa, Shizuoka prefecture, Japan (ca. 100 m from the aviary and 200 m from the vegetable gardens). Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 09/24/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1086 Species: Caenorhabditis remanei Genotype: C. remanei wild isolate. Description: Male-female strain. Isolated by Marie-Anne Felix from leaf litter/bark/soil sampled on March 14, 2007, in the woods behind the parking lot of the Kacho-en aviary in Kakegawa, Shizuoka prefecture, Japan (ca. 100 m from the aviary and 200 m from the vegetable gardens). Isofemale line. Maintain by mating. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 09/24/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1087 Species: Caenorhabditis remanei Genotype: C. remanei wild isolate. Description: Male-female strain. Isolated by Marie-Anne Felix from leaf litter/bark/soil sampled on March 18, 2007, in the Botanical Gardens of the University of Tokyo in Tokyo, Japan. Isofemale line. Maintain by mating. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 09/24/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1088 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated by Marie-Anne Felix from soil sampled on March 14, 2007, in the Kacho-en aviary in Kakegawa, Shizuoka prefecture, Japan. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 09/24/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1171 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Caenorhabditis elegans wild isolate. Isolated from a compost sample collected in April 2007 in Concepcion, Chile, in the Palomares area, 2 km NE of the town. Mutagen: Outcrossed: x Made by: M-A Felix Received: 11/26/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1172 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated from a sample of soil (compost) in a pot with rotting tomatoes collected in April 2007 in Concepcion, Chile, in the Villuco area, 3 km SE of the town. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 02/16/10 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1184 Species: Caenorhabditis remanei Genotype: mfEx34. Description: Male-female strain. mfEx34 [Cel-sid-2 + Cel-myo-2::DsRed]. Made by injection of PB4641 with a PCR product of the Cel-sid-2 gene and a myo-2::DsRed plasmid. Sensitive to RNAi by feeding. Mutagen: Outcrossed: x Made by: Isabelle Nuez Received: 09/24/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1199 Species: Caenorhabditis afra Genotype: Caenorhabditis afra wild isolate. Description: Caenorhabditis sp. 7 Male-female strain. Isolated by Marie-Anne Felix from rotting citrus fruit sampled by M. Herrmann in Begoro, Ghana in June 2007. New male-female species of the elegans group. Does not produce cross-progeny with any of the other previous elegans group species. Isofemale line. Maintain by mating. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 09/24/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1200 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated by Tony Page from a compost heap sample recovered on 1 Aug 2007 in SouthWest Scotland 4°36.0' West 55°34.6' North. Mutagen: Outcrossed: x Made by: Tony Page Received: 02/16/10 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1201 Species: Caenorhabditis sinica Genotype: Caenorhabditis sinica wild isolate. Description: Caenorhabditis sinica wild isolate. Caenorhabditis sp. 5 Male-female strain. Isolated from a small fruit found in the Garden of Harmony in Suzhou, China, on August 17, 2007. Mutagen: Outcrossed: x Made by: Received: 10/09/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1202 Species: Caenorhabditis sinica Genotype: Caenorhabditis sinica wild isolate. Description: Caenorhabditis sinica wild isolate. Caenorhabditis sp. 5 Male-female strain. Isolated from a small fruit found in the Feilaifeng Park in Hangzhou, China, on August 25, 2007. Similar fruit as JU1201. Mutagen: Outcrossed: x Made by: Received: 10/09/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1212 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: M-A. Felix Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: JU1213 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: M-A. Felix Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: JU1242 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: M-A. Felix Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: JU1246 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: M-A. Felix Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: JU1286 Species: Caenorhabditis afra Genotype: Caenorhabditis afra wild isolate. Description: Caenorhabditis sp. 7 Male-female strain. Caenorhabditis sp. 7 is currently an undescribed species. The sequenced strain, JU1286, is an isogenic inbred line derived from strain JU1199 by 20 consecutive matings of 1 virgin female and 1 male. The inbreeding was done by Marie-Anne Felix. Strain JU1199 was isolated in June 2007 by Matthias Herrmann from a rotting citrus fruit in Begoro, Ghana, Africa. C. sp. 7 is a gonochoristic species with about equal proportions of males and females. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 09/15/11 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1325 Species: Caenorhabditis nigoni Genotype: Caenorhabditis nigoni wild isolate. Description: Caenorhabditis sp. 9 Male-female strain. Isolated from rotting flowers and leaves sampled in the Zoo/Botanical Garden of Trivandrum, Kerala, India on 21 Dec 2007. Culture at 20°C or above. Reference: Félix, Braendle & Cutter, 2014 Mutagen: Outcrossed: x0 Made by: Marie-Anne Felix Received: 04/23/14 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1333 Species: Caenorhabditis doughertyi Genotype: Caenorhabditis doughertyi wild isolate. Description: Caenorhabditis sp. 10 Male-female strain. Isolated by Marie-Anne Félix from rotting cacao fruit sampled in Angela Spice Garden a few kilometers from Periyar, Kerala, India on 27 Dec 2007. Culture at 20°C or above. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 03/11/10 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1341 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: Hermaphrodite. Isolated by MAF from rotting small red fruits (same as JU1342) and bark in the forest near the top of the mountain in Ponmudi, Kerala, India on 22 Dec 2007 (ca. 20 m from JU1340). Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 06/13/12 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1345 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: Hermaphrodite. Isolated by MAF from dark brown "fruits" (1x1.5 cm) on fallen tree trunk in the forest near the top of the mountain in Ponmudi, Kerala, India on 22 Dec 2007. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 06/13/12 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1348 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: Hermaphrodite. Isolated by MAF from a mix of rotting fruits, leaf litter, soil, bark, flowers, etc. sampled in a ca. 3 km wide area in Periyar Natural Preserve, Kerala, India on 28 Dec 2007. Isolated from a plate containing a rotting fruit similar to that of sample #46 - JU1361/2). Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 06/13/12 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1373 Species: Caenorhabditis tropicalis Genotype: Caenorhabditis tropicalis wild isolate. Description: Caenorhabditis sp. 11 Isolated by Marie-Anne Felix from rotting torch ginger (Etlingeria elatior) flowers sampled in the island of La Réunion by Valérie Robert and Loïc Sablé in Jan 2008. Hermaphrodite. Culture at 20°C or above. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 03/11/10 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1395 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: M-A. Felix Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: JU1400 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated from rotting orange fruits, Garden Catalina de Ribera, Sevilla, Spain sampled on 29 Mar 08 by MAF. Not bleached. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 02/16/10 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1401 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated from Rumina decollata snail #4, sampled in front of the Parador of Carmona, Spain on 31 Mar 08 by MAF. Plated 3 Apr 08. Isolated as adult on 9 Apr 08. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 02/16/10 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1409 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: M-A. Felix Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: JU1422 Species: Caenorhabditis nigoni Genotype: Caenorhabditis nigoni wild isolate. Description: Caenorhabditis sp. 9 Male-female strain. Derived by 25 rounds of inbreeding (1 virgin female + 1 male) from JU1325, isolated from rotting flowers and leaves sampled in the Zoo/Botanical Garden of Trivandrum, Kerala, India on 21 Dec 2007. Culture at 20°C or above. Mutagen: Outcrossed: x25 Made by: Marie-Anne Felix Received: 09/15/11 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1426 Species: Caenorhabditis castelli Genotype: Caenorhabditis castelli wild isolate. Description: Caenorhabditis sp. 12 Male-female strain. Isolated in Nouragues, French Guiana. Reference: Kiontke KC, et al. BMC Evol Biol. 2011 Nov 21;11:339. Mutagen: Outcrossed: x Made by: M-A Felix Received: 04/01/12 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1427 Species: Caenorhabditis castelli Genotype: Caenorhabditis castelli wild isolate. Description: Caenorhabditis sp. 12 Male-female strain. Isolated in May 2008 from rotting Micropholis cayennensis fruit (#1, subsample L), sampled by P. Châtelet on the "Petit Plateau" near the CNRS Biological station, Nouragues, French Guyana. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 01/26/11 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1428 Species: Caenorhabditis tropicalis Genotype: Caenorhabditis tropicalis wild isolate. Description: Caenorhabditis sp. 11 Isolated by Marie-Anne Felix from rotting Duguetia surinamensis fruit, sampled by Patrick Châtelet on the "Petit Plateau" in the Nouragues Forest, French Guyana in May 2008. Hermaphrodite. Culture at 20°C or above. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 03/11/10 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1440 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated from rotting palm fruit sampled by MAF on 9 June 2008 in the Park Guëll, Barcelona, Spain. Picked as young adult on 11 June 2008. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 02/16/10 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1491 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: M-A. Felix Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: JU1530 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: M-A. Felix Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: JU1568 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: M-A. Felix Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: JU1580 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: M-A. Felix Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: JU1581 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: M-A. Felix Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: JU1586 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: M-A. Felix Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: JU1593 Species: Caenorhabditis afra Genotype: Caenorhabditis afra wild isolate. Description: Caenorhabditis sp. 7 Male-female strain. Isolated from leaf litter near pond edge, sampled by J. and G. Hatty on 29 Nov 08 near Shonga, Nigeria. Mutagen: Outcrossed: x Made by: J Hatty & M-A. Felix Received: 08/17/11 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1615 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated by Matthew Crook from compost heap, 13 Cherry St., Macleod, Melbourne, Australia, March 2009. *** NOTE: JU1615 is probably a N2 contaminant and not a real wild isolate. This is inferred from genotyping data from Erik Andersen et al. in the Kruglyak lab. (M-A Felix, Dec 2010). Mutagen: Outcrossed: x Made by: Matthew Crook Received: 02/16/10 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1652 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated by Rosina Giordano from compost sampled in Montevideo, Uruguay. Mutagen: Outcrossed: x Made by: Rosina Giordano Received: 02/16/10 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1667 Species: Caenorhabditis monodelphis Genotype: Caenorhabditis monodelphis wild isolate. Description: Inbred line from wild isolate SB341. Inbred for 20 generations by sib mating L4 female and male. Previously known as Caenorhabditis sp. 1. Mutagen: Outcrossed: x20 Made by: M-A. Felix Received: 12/12/12 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1771 Species: Caenorhabditis doughertyi Genotype: Caenorhabditis doughertyi wild isolate. Description: Caenorhabditis sp. 10 Male-female strain. Inbred line from JU1333. Inbred for 25 generations by brother-sister (L4) mating. Mutagen: Outcrossed: x25 Made by: M-A. Felix Received: 12/12/12 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1825 Species: Caenorhabditis nouraguensis Genotype: Caenorhabditis nouraguensis wild isolate. Description: Caenorhabditis sp. 17. Maintain at 23C. Isolated from rotten wide bean of Barokia sp. sampled on Grand Plateau in Nouragues Forest, French Guiana, on 21 Nov 2009. Reference: Félix, Braendle & Cutter, 2014 Mutagen: Outcrossed: x0 Made by: Marie-Anne Felix Received: 04/23/14 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1857 Species: Caenorhabditis macrosperma Genotype: Caenorhabditis macrosperma wild isolate. Description: Caenorhabditis sp. 18 Male-female strain. Isolated in Nouragues, French Guiana. Reference: Kiontke KC, et al. BMC Evol Biol. 2011 Nov 21;11:339. Mutagen: Outcrossed: x Made by: Christian Braendle Received: 04/01/12 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1873 Species: Caenorhabditis wallacei Genotype: Caenorhabditis wallacei wild isolate. Description: Isolated in Sanda Center, Indonesia . Reference: Kiontke KC, et al. BMC Evol Biol. 2011 Nov 21;11:339. Mutagen: Outcrossed: x Made by: J-B Penigault Received: 04/01/12 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1896 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: M. Barkoulas Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: JU1904 Species: Caenorhabditis wallacei Genotype: Caenorhabditis wallacei wild isolate. Description: Caenorhabditis sp. 16 25X inbred derivative of JU1873. Use for genome sequencing. Mutagen: Outcrossed: x Made by: J-B. Pénigault Received: 08/17/11 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1905 Species: Caenorhabditis imperialis Genotype: Caenorhabditis imperialis wild isolate. Description: Caenorhabditis sp. 14 Male-female strain. Isolated in Petit-Bourg, Guadeloupe. Reference: Kiontke KC, et al. BMC Evol Biol. 2011 Nov 21;11:339. Mutagen: Outcrossed: x Made by: J. Gomes & M-A Felix Received: 04/01/12 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU1968 Species: Caenorhabditis virilis Genotype: Caenorhabditis virilis wild isolate. Description: Caenorhabditis sp. 13 Male-female strain. Inbred derivative of JU1528. Derived by sib mating (1 virgin female + 1 male) for 25 generations. Mutagen: Outcrossed: x Made by: M-A. Felix Received: 08/17/11 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU2039 Species: Caenorhabditis elegans Genotype: mfIs70 IV; rde-1(ne219) V. Description: mfIs70 [lin-31p::rde-1 + myo2p::GFP]. mfIs70 is a spontaneous integration in LG IV. Superficially wild-type. This strain can be used for Pn.p-specific RNAi. Reference: Barkoulas et al. (2013) Developmental Cell Jan 14;24(1):64-75 Mutagen: Spontaneous Integrant Outcrossed: x0 Made by: Michalis Barkoulas Received: 03/15/25 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU2058 Species: Caenorhabditis elegans Genotype: rrf-3(pk1426) II; mfIs70 IV; rde-1(ne219) V. Description: mfIs70 [lin-31p::rde-1 + myo2p::GFP]. mfIs70 is a spontaneous integration in LG IV. Superficially wild-type. This strain can be used for Pn.p-specific RNAi. Reference: Barkoulas et al. (2013) Developmental Cell Jan 14;24(1):64-75 Mutagen: Spontaneous Integrant Outcrossed: x0 Made by: Michalis Barkoulas Received: 03/15/25 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU2079 Species: Caenorhabditis nouraguensis Genotype: Caenorhabditis nouraguensis wild isolate. Description: Caenorhabditis sp. 17 Male-female strain. Isogenic wild type line derived from JU1825. 28 rounds of sib mating with virgin females. Use as reference. Mutagen: Outcrossed: x Made by: M-A. Felix Received: 08/17/11 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU2083 Species: Caenorhabditis macrosperma Genotype: Caenorhabditis macrosperma wild isolate. Description: Caenorhabditis sp. 18 Male-female strain. Isogenic wild type line derived from JU1857. 25 rounds of sib mating with virgin females. Mutagen: Outcrossed: x Made by: M-A. Felix Received: 08/17/11 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU2156 Species: Caenorhabditis zanzibari Genotype: Caenorhabditis zanzibari wild isolate. Description: Isolated by M.-A. Félix from rotting fruits of Calophyllum inophyllum (red mahogany) sampled in the Jozani National Forest, Zanzibar, Tanzania (6.2715°S, 39.416°E), on 3/9/2012. Previously known as Caenorhabditis sp. 26. Mutagen: Outcrossed: x Made by: M-A. Felix Received: 02/28/13 New York University, New York ----------------------------------------------------------------------------- Strain: JU2161 Species: Caenorhabditis zanzibari Genotype: Caenorhabditis zanzibari wild isolate. Description: Male-Female strain. Isolated by M.-A. Félix from a rotting mandarin sampled on a farm in the Dole district, Zanzibar, Tanzania (6.1074°S, 39.2515°E) , on 3/7/2012. Previously known as Caenorhabditis sp. 26. Mutagen: Outcrossed: x Made by: M-A. Felix Received: 02/28/13 New York University, New York ----------------------------------------------------------------------------- Strain: JU2190 Species: Caenorhabditis zanzibari Genotype: Caenorhabditis zanzibari wild isolate. Description: Male-Female strain. 26 rounds of sib mating (L4 female) of JU2161. Healthy. Use for genome sequencing. Previously known as Caenorhabditis sp. 26. Mutagen: Outcrossed: x26 Made by: M-A. Felix Received: 12/12/12 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU2469 Species: Caenorhabditis uteleia Genotype: Caenorhabditis uteleia wild isolate. Description: Male-female strain. From a rotting kumquat-like fruit collected by Ludmilla Lokmane in Madre de Dios (Peru) in Jan 2013. Isolated by Lise Frézal. Line started from an adult isolated on 9 Jan 2013. Previously known as Caenorhabditis sp. 31. Mutagen: Outcrossed: x0 Made by: Marie-Anne Félix Received: 11/27/13 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU258 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: From Madeira, Ribeiro Frio. Fruit and vegatable gardens. Caenorhabditis elegans wild isolate. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 05/15/02 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU2585 Species: Caenorhabditis uteleia Genotype: Caenorhabditis uteleia wild isolate. Description: Male-female strain. Inbred line derived from JU2469 by 25 rounds of brother-sister mating using a L4 female larva and a male. Healthy. Use for genome sequencing. Previously known as Caenorhabditis sp. 31. Mutagen: Outcrossed: x Made by: Marie-Anne Félix Received: 01/22/15 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU262 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: From Le Blanc (Indre, France). From a vegetable garden garbage pile. Weak Phm phenotype. Caenorhabditis elegans wild isolate. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 05/15/02 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU263 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: From Le Blanc (Indre, France). From a vegetable garden garbage pile. Same soil sample as JU262. Old adults in this strain get vacuoles in their intestinal cells. Caenorhabditis elegans wild isolate. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 05/15/02 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU2745 Species: Caenorhabditis quiockensis Genotype: Caenorhabditis quiockensis wild isolate. Description: Male-female strain. Isolated from a rotting fruit sampled on 09 June 2014 by Fabrice Besnard in Guadeloupe (Basse Terre, 16.1761, -61.6951). Started with a plugged female. Previously known as Caenorhabditis sp. 38. Mutagen: Outcrossed: x Made by: Lise Frézal Received: 01/22/15 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU2774 Species: Caenorhabditis tribulationis Genotype: Caenorhabditis tribulationis wild isolate. Description: Isolated from humus sampled on 08 August 2014 below the cathedral fig tree Ficus destruens by Danbulla Road, Australia (-17.1774, 145.6600) by Jean-Baptiste Pénigault. Started on 19 August from a plugged female. Crossed with sp. 5 JU727 in both directions; the outcome is more than 100 embryos, no larvae. Previously known as Caenorhabditis sp. 40. Mutagen: Outcrossed: x Made by: Lise Frézal Received: 01/22/15 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU2788 Species: Caenorhabditis sulstoni Genotype: Caenorhabditis sulstoni wild isolate. Description: Inbred line derived from SB454 by 25 rounds of brother-sister mating using a L4 female larva and a male. Healthy. Use for genomic sequencing. Previously known as Caenorhabditis sp. 32. Mutagen: Outcrossed: x Made by: Marie-Anne Félix Received: 01/22/15 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU279 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: From Jardin des Plantes, Paris, France, 6 Aug 02. Isolated from a decomposing Helix aspersa snail containing maggots (same snail as JU280, JU296). Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 03/01/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU2809 Species: Caenorhabditis quiockensis Genotype: Caenorhabditis quiockensis wild isolate. Description: Inbred line derived from JU2745 by 25 rounds of brother-sister mating using a L4 female larva and a male. Used for DNA/RNA sequencing. Previously known as Caenorhabditis sp. 38. Mutagen: Outcrossed: x Made by: Lise Frézal Received: 01/22/15 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU2818 Species: Caenorhabditis tribulationis Genotype: Caenorhabditis tribulationis wild isolate. Description: Inbred line derived from JU2774 by 25 rounds of brother-sister mating using a L4 female larva and a male. Used for DNA/RNA sequencing. Previously known as Caenorhabditis sp. 40. Mutagen: Outcrossed: x Made by: Lise Frézal Received: 01/22/15 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU298 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated by Marie-Anne Felix. Le Blanc, Indre, France, in a vegetable garden compost pile, August 25, 2002. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 10/09/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU299 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Le Blanc, Indre, France in a vegetable garden compost pile on August 25, 2002. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU300 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Le Blanc, Indre, France in a vegetable garden compost pile on August 25, 2002. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU301 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Le Blanc, Indre, France in a vegetable garden compost pile on August 25, 2002. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU302 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Le Blanc, Indre, France in a vegetable garden compost pile on August 25, 2002. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU303 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Le Blanc, Indre, France in a vegetable garden compost pile on August 25, 2002. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU304 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Le Blanc, Indre, France in a vegetable garden compost pile on August 25, 2002. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU305 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Le Blanc, Indre, France in a vegetable garden compost pile on August 25, 2002. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU306 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Le Blanc, Indre, France in a vegetable garden compost pile on August 25, 2002. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU307 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Le Blanc, Indre, France in a vegetable garden compost pile on August 25, 2002. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU308 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Le Blanc, Indre, France in a vegetable garden compost pile on August 25, 2002. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU309 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Le Blanc, Indre, France in a vegetable garden compost pile on August 25, 2002. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU310 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Le Blanc, Indre, France in a vegetable garden compost pile on August 25, 2002. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU311 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated by Marie-Anne Felix. Merlet, Lagorce (Ardeche), France, on September 8, 2002, in wash from snail tube - snails coming from under a tree full of ivy. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 10/09/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU312 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 in the wash from snail tube - snails coming from under a tree full of ivy. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU313 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 in the wash from snail tube - snails coming from under a tree full of ivy. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU314 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002. Snails resembling Helix, under a tree full of ivy. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU3143 Species: Mesorhabditis okuensis Genotype: Mesorhabditis okuensis wild isolate. Description: Mesorhabditis okuensis wild isolate. Male-female strain: maintain by crossing. Typically very few males on plates (only 10%); might be necessary to transfer large chunks to the new plates to ensure enough males are transferred. This species displays a striking reproductive system (described in Grosmaire & al. Science 2019. doi: 10.1126/science.aau0099). Mutagen: Outcrossed: x0 Made by: Marie-Anne Félix Received: 11/12/19 Ecole Normale Superieure de Lyon, Lyon, France ----------------------------------------------------------------------------- Strain: JU315 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from Pomatias elegans (?) snails. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU316 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from Pomatias elegans (?) snails. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU317 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from destroyed snail Oxychilus sp. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU318 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from soil under a tree full of ivy. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU319 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated on Sept 8, 2002, from garden soil in Merlet, Lagorce (Ardeche), France. See Barriere & Felix, Current Biology 2005 (sample Merlet 1). Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 07/20/05 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU320 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from soil under a tree full of ivy. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU321 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from soil under a tree full of ivy. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU322 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated on Sept 8, 2002, from a Helix snail on the trunk of a mulberry tree in Merlet, Lagorce (Ardeche), France. See Barriere & Felix, Current Biology 2005 (sample Merlet 2). Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 07/20/05 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU323 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from a Helix (?) snail #3 on the trunk of a mulberry tree (not the same snail as JU322). Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU342 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from the wash from a snail tube (mulberry tree). Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU343 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from a Glomeris myriapod in the compost pile (same myriapod as JU344, JU345 and JU346). Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU344 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from a Glomeris myriapod in the compost pile (same myriapod as JU343, JU345 and JU346). Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU345 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated on Sept 8, 2002, from a Glomeris myriapod in a compost pile in Merlet, Lagorce (Ardeche), France. See Barriere & Felix, Current Biology 2005 (sample Merlet 3). Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 07/20/05 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU346 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from a Glomeris myriapod in the compost pile (same myriapod as JU342, JU343 and JU344). Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU347 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Merlet, Lagorce (Ardche), France on September 8, 2002 from a Pomatias elegans (?) snail, under the mulberry tree. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU348 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: From Merlet, Lagorce (Ardèche), France. 8 Sep 02. From a Oxychilus sp. snail , under the mulberry tree. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 03/01/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU360 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated by Marie-Anne Felix. Franconville (Val d'Oise), France, in a compost heap in Claude Pieau's gargen, on September 16, 2002. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 10/09/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU361 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated by Marie-Anne Felix. Franconville (Val d'Oise), France, in a compost heap in Claude Pieau's gargen, on September 16, 2002. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 10/09/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU362 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Franconville (Val d Oise), France on September 16, 2002 from a compost heap in Claude Pieau's garden. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU363 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated by Marie-Anne Felix. Franconville (Val d'Oise), France, in a compost heap in Claude Pieau's gargen, on September 16, 2002. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 10/09/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU364 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Franconville (Val d Oise), France on September 16, 2002 from a compost heap in Claude Pieau's garden. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU365 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Franconville (Val d Oise), France on September 16, 2002 from a compost heap in Claude Pieau's garden. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU366 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Franconville (Val d Oise), France on September 16, 2002 from a compost heap in Claude Pieau's garden. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU367 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Franconville (Val d Oise), France on September 16, 2002 from a compost heap in Claude Pieau's garden. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU368 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Franconville (Val d Oise), France on September 16, 2002 from a compost heap in Claude Pieau's garden. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU369 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Franconville (Val d Oise), France on September 16, 2002 from a compost heap in Claude Pieau's garden. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU370 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Franconville (Val d Oise), France on September 16, 2002 from a compost heap in Claude Pieau's garden. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU371 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Franconville (Val d Oise), France on September 16, 2002 from a compost heap in Claude Pieau's garden. Mutagen: Outcrossed: x Made by: MA Felix Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU372 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: From Viosne Valley, next to Santeuil, Val d'Oise. France. Isolated from embankment on the road, below a cultivated field. 22 Sep 02. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 03/01/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU393 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated in Hermanville (Calvados), France. Compost pile. Sept 02. Mutagen: Outcrossed: x Made by: Antione Barriere Received: 06/13/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU394 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated in Hermanville (Calvados), France. Compost pile. Sept 02. Mutagen: Outcrossed: x Made by: Antione Barriere Received: 06/13/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU395 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated in Hermanville (Calvados), France. Compost pile. Sept 02. Mutagen: Outcrossed: x Made by: Antione Barriere Received: 06/13/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU396 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Hermanville (Calvados) , France in September, 2002 from a compost pile. Mutagen: Outcrossed: x Made by: Antoine Barrière Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU397 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Hermanville (Calvados) , France in September, 2002 from a compost pile. Mutagen: Outcrossed: x Made by: Antoine Barrière Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU398 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated in Hermanville (Calvados), France. Compost pile. Sept 02. Mutagen: Outcrossed: x Made by: Antione Barriere Received: 06/13/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU399 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Hermanville (Calvados) , France in September, 2002 from a compost pile. Mutagen: Outcrossed: x Made by: Antoine Barrière Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU400 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated in Hermanville (Calvados), France. Compost pile. Sept 02. Mutagen: Outcrossed: x Made by: Antione Barriere Received: 06/13/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU401 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Hermanville (Calvados) , France in September, 2002 from a compost pile. Mutagen: Outcrossed: x Made by: Antoine Barrière Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU402 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated in Hermanville (Calvados), France. Compost pile. Sept 02. Mutagen: Outcrossed: x Made by: Antione Barriere Received: 06/13/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU403 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: From Hermanville (Calvados), France, from a compost pile, Sept 2002. Mutagen: Outcrossed: x Made by: Antoine Barrière Received: 03/01/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU406 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: C. elegans wild isolate. Isolated in Hermanville (Calvados) , France on December 30, 2002 from a compost pile. Mutagen: Outcrossed: x Made by: Antoine Barrière Received: 08/13/08 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU407 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated in Hermanville (Calvados), France. Compost pile. Sept 02. Mutagen: Outcrossed: x Made by: Antione Barriere Received: 06/13/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU42 Species: Oscheius tipulae Genotype: Oscheius tipulae unc-2(mf29). Description: Sluggish Unc. Recessive autosomal. Parental strain Oscheius tipulae CEW1. AKA Oscheius sp. 1. Mutagen: EMS Outcrossed: x5 Made by: M Felix/M Dichtel Received: 03/01/01 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU438 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated in Hermanville (Calvados), France. Compost pile. Sept 02. Mutagen: Outcrossed: x Made by: Antione Barriere Received: 06/13/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU439 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: From ReykjavIk cemetery (Iceland). Collected on August 10, 2003. Mutagen: Outcrossed: x Made by: Antoine Barrière Received: 03/01/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU440 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated by Dorothee Baille. Beauchene (Eure & Loir), France, in a compost pile, on September 12, 2003. Mutagen: Outcrossed: x Made by: Dorothee Baille Received: 10/09/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU441 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: From Beauchêne (Eure & Loir -France), in the cabbage parcel, 12 Sep 03. Mutagen: Outcrossed: x Made by: David Baillie Received: 03/01/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU486 Species: Caenorhabditis elegans Genotype: mfIs4. Description: mfIs4 [egl-17::YFP + daf-6::CFP + unc-119(+)]. YFP is expressed in the secondary vulval lineage (vulC, D) and CFP in the primary vulval lineage (vulE, F). egl-17::YFP from the pDRS17 plasmid (D. Sherwood and P. Sternberg). daf-6::CFP from the pCK1 plasmid (C. Kolditz and MA Felix). Slightly Egl, Pvl. unc-119(ed3) might still be present in the background. Mutagen: Gamma Rays Outcrossed: x10 Made by: MA Felix/C Braendle Received: 07/20/05 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU516 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: Isolated from compost heap in Marsas (Hautes-Pyrénées, France) on August 15, 2004. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 03/01/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU533 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated from compost heap in Primel-Trégastel (Finistère, France), 03 Oct 04. Picked as adult on 6 Oct 04. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 02/16/10 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU561 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: M-A. Felix Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: JU642 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated from the compost heap in Jean-Antoine Lepesant's garden (Le Perreux sur Marne -94- France), 14 Dec 04. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 02/16/10 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU724 Species: Caenorhabditis remanei Genotype: C. remanei wild isolate. Description: Male-female strain. Isolated on May 13, 2005 in Zhouzhuang, Jiangsu, China from soil in cultivated fields (beans, cereal) at the SW entrance of the village. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 07/20/05 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU725 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: Isolated on May 4, 2005 from mushroom compost in a farmyard 1 km south of Yangshuo, Guangxi, China. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 07/20/05 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU726 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: Isolated on May 6, 2005 from soil with freshly added compost in a cabbage field in Chengyang Village, 20 km north of Sanjiang, Guangxi, China. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 07/20/05 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU727 Species: Caenorhabditis species Genotype: Caenorhabditis sp. wild isolate. Description: Male-female strain. Isolated on May 6, 2005 under a tree along a path between two villages in Changyang, 20 km north of Sanjiang, Guangxi, China. Does not cross with C. remanei PB4641 or JU724, nor with C. sp. PB2801. sp. 5 in Kiontke and Sudhaus Wormbook Ecology chapter. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 07/20/05 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU751 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: M-A. Felix Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: JU757 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: Isolated in Le Blanc (12 June 2005), from a compost heap. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 03/01/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU774 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated in Carcavelos, Portugal from garden garbage left on pavement, 10 July 05. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 02/16/10 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU775 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated in the Botanical Garden, Lisbon, Portugal. July 10, 2005 below a tree with red fruits rotting. Mutagen: Outcrossed: x Made by: Marie Anne Felix Received: 06/13/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU776 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated in the Botanical Garden, Lisbon, Portugal. July 10, 2005 below a tree with red fruits rotting. Mutagen: Outcrossed: x Made by: Marie Anne Felix Received: 06/13/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU777 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated in the Botanical Garden, Lisbon, Portugal. July 10, 2005 below a tree with red fruits rotting. Mutagen: Outcrossed: x Made by: Marie Anne Felix Received: 06/13/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU778 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated in the Botanical Garden, Lisbon, Portugal. July 10, 2005 below a Ficus isophlebia tree with fruit. Mutagen: Outcrossed: x Made by: Marie Anne Felix Received: 06/13/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU779 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated in the Botanical Garden, Lisbon, Portugal. July 10, 2005 below a Ficus isophlebia tree with fruit. Mutagen: Outcrossed: x Made by: Marie Anne Felix Received: 06/13/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU780 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated in the Botanical Garden, Lisbon, Portugal. July 10, 2005 below a Ficus isophlebia tree with fruit. Mutagen: Outcrossed: x Made by: Marie Anne Felix Received: 06/13/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU781 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated in the Botanical Garden, Lisbon, Portugal. July 10, 2005 below a Ficus isophlebia tree with fruit. Mutagen: Outcrossed: x Made by: Marie Anne Felix Received: 06/13/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU782 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: M-A. Felix Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: JU792 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: M-A. Felix Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: JU793 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: Isolated from the compost heap at the top of the garden in Frechendets (Hautes Pyrénées), on August 31, 2005. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 03/01/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU799 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Isolated in the Botanical Garden, Lisbon, Portugal. July 10, 2005 below a tree with red fruits rotting. Mutagen: Outcrossed: x Made by: Marie Anne Felix Received: 06/13/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU800 Species: Caenorhabditis species Genotype: Caenorhabditis sp. wild isolate. Description: Male-female strain. Inbred derivative of JU727. Derived from JU727 by 20 generations of sib mating. Male/female strain. Isolated on May 6, 2005 under a tree along a path between two villages in Changyang, 20 km north of Sanjiang, Guangxi, China. Does not cross with C. remanei PB4641 or JU724, nor with C. sp. PB2801. sp. 5 in Kiontke and Sudhaus Wormbook Ecology chapter. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 11/10/06 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU829 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Compost heap of Ray Hong in Tübingen, Germany, 28 Sep 05. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 02/16/10 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU830 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: R. Hong Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: JU835 Species: Caenorhabditis briggsae Genotype: C. briggsae wild isolate. Description: Isolated from a vegetable garden/orchard near Obernai (Bas-Rhin), France on October 2, 2005. Mutagen: Mos1 Outcrossed: x5 Made by: Marie-Anne Felix Received: 03/01/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU847 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Maintain under normal conditions. Reference: Andersen EC, Nat Genet. 2012 Jan 29;44(3):285-90. Mutagen: Outcrossed: x0 Made by: M-A. Felix Received: 10/03/12 University of California, Los Angeles, CA ----------------------------------------------------------------------------- Strain: JU852 Species: Caenorhabditis elegans Genotype: C. elegans wild isolate. Description: Sampled in a compost heap in vegetable gardens/vineyards (Bas-Rhin), France on 3 Oct 05 by MAF. Plated 4 Oct, picked as L4 on 4 Oct 05. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 02/16/10 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JU929 Species: Caenorhabditis briggsae Genotype: Cbr-dpy-18(mf104). Description: Mos1 insertion in CBG13195 = Cbr-dpy-18 in exon before TATgtgagtcgatttgtttgatcgg. Dpy phenotype. Mutagen: Outcrossed: x Made by: Marie-Anne Felix Received: 03/01/07 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JUb134 Species: Sphingomonas molluscorum Genotype: Sphingomonas molluscorum Description: Bacteria. CeMbio Collection. Natural isolate from a C. elegans population in rotting stem. Sampled in: Santeuil, France. Slow grower. More information about collection on the project's wiki: http://www.cembio.uni-kiel.de/. 16S rRNA primer: 27F/1492R. 16S rRNA sequence: CTTGAGAGTTTGATCCTGGCTCAGAACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGAGACCTTCGGGTCTAGTGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTTGGGTTCGGAATAACAGTTGGAAACGACTGCTAATACCGGATGATGACGTAAGTCCAAAGATTTATCGCCCAGGGATGAGCCCGCGTAGGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCCTTAGCTGGTCTGAGAGGATGATCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCAATGCCGCGTGAGTGATGAAGGCCTTAGGGTTGTAAAGCTCTTTTACCCGGGATGATAATGACAGTACCGGGAGAATAAGCCCCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTAGGCGGCTTTGTAAGTTAGAGGTGAAAGCCTGGAGCTTAACTCCAGAACTGCCTTTAAGACTGCATCGCTTGAATCCAGGAGAGGTGAGTGGAATTCCGAGTGTAGAGGTGAAATTCGTAGATATTCGGAAGAACACCAGTGGCGAAGGCGGCTCACTGGACTGGTATTGACGCTGAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATAACTAGCTGTCCGGGTGCTTGGCATTTGGGTGGCGCAGCTAACGCATTAAGTTATCCGCCTGGGGAGTACGGTCGCAAGATTAAAACTCAAAGGAATTGACGGGGGCCTGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGCAGAACCTTACCAGCGTTTGACATGGTAGGACGACTGGCAGAGATGCCTTTCTTCCCTTCGGGGACCTACACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCGCCTTTAGTTACCATCATTTAGTTGGGTACTCTAAAGGAACCGCCGGTGATAAGCCGGAGGAAGGTGGGGATGACGTCAAGTCCTCATGGCCCTTACGCGCTGGGCTACACACGTGCTACAATGGCAACTACAGTGGGCAGCAATCCCGCGAGGGTGAGCTAATCTCCAAAAGTTGTCTCAGTTCGGATTGTTCTCTGCAACTCGAGAGCATGAAGGCGGAATCGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCAGGCCTTGTACACACCGCCCGTCACACCATGGGAGTTGGATTCACCCGAAGGCGTTGCGCCAACCCGCAAGGGGAGCAGGCGACCACGGTGGGTTCAGCGACTGGGGTGAAGTCGTAACAAGGTAGCCGTAGGGGAACCTGCGGCTGGATCACCTCCTTT GenBank: BioSample SAMN10361612 Mutagen: Outcrossed: x0 Made by: Wild isolate Received: 07/17/19 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JUb19 Species: Stenotrophomonas maltophilia Genotype: Stenotrophomonas maltophilia Description: Bacteria. CeMbio Collection. Natural isolate from C. elegans natural habitat (Rotting pear). LB, 20-26C. Sampled in Le Blanc, France. More information about collection on the project's wiki: http://www.cembio.uni-kiel.de/. 16S rRNA primer: 27F/1492R. 16S rRNA sequence: TGAAGAGTTTGATCCTGGCTCAGAGTGAACGCTGGCGGTAGGCCTAACACATGCAAGTCGAACGGCAGCACAGAGGAGCTTGCTCCTTGGGTGGCGAGTGGCGGACGGGTGAGGAATACATCGGAATCTACTTTTTCGTGGGGGATAACGTAGGGAAACTTACGCTAATACCGCATACGACCTACGGGTGAAAGCAGGGGACCTTCGGGCCTTGCGCGATTGAATGAGCCGATGTCGGATTAGCTAGTTGGCGGGGTAAAGGCCCACCAAGGCGACGATCCGTAGCTGGTCTGAGAGGATGATCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGATCCAGCCATACCGCGTGGGTGAAGAAGGCCTTCGGGTTGTAAAGCCCTTTTGTTGGGAAAGAAATCCAGCCGGCTAATACCTGGTTGGGATGACGGTACCCAAAGAATAAGCACCGGCTAACTTCGTGCCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTAGGTGGTTGTTTAAGTCTGTTGTGAAAGCCCTGGGCTCAACCTGGGAACTGCAGTGGAAACTGGACAACTAGAGTGTGGTAGAGGGTAGCGGAATTCCCGGTGTAGCAGTGAAATGCGTAGAGATCGGGAGGAACATCCATGGCGAAGGCAGCTACCTGGACCAACACTGACACTGAGGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCCTAAACGATGCGAACTGGATGTTGGGTGCAATTTGGCACGCAGTATCGAAGCTAACGCGTTAAGTTCGCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGTATGTGGTTTAATTCGATGCAACGCGAAGAACCTTACCTGGCCTTGACATGTCGAGAACTTTCCAGAGATGGATTGGTGCCTTCGGGAACTCGAACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTCCTTAGTTGCCAGCACGTAATGGTGGGAACTCTAAGGAGACCGCCGGTGACAAACCGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGGCCAGGGCTACACACGTACTACAATGGTAGGGACAGAGGGCTGCAAGCCGGCGACGGTAAGCCAATCCCAGAAACCCTATCTCAGTCCGGATTGGAGTCTGCAACTCGACTCCATGAAGTCGGAATCGCTAGTAATCGCAGATCAGCATTGCTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGGGAGTTTGTTGCACCAGAAGCAGGTAGCTTAACCTTCGGGAGGGCGCTTGCCACGGTGTGGCCGATGACTGGGGTGAAGTCGTAACAAGGTAGCCGTATCGGAAGGTGCGGCTGGATCACCTCCTTT Mutagen: Outcrossed: x0 Made by: Wild isolate Received: 07/17/19 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JUb44 Species: Chryseobacterium sp. Genotype: Chryseobacterium sp. Description: Bacteria. CeMbio Collection. Natural isolate from C. elegans natural habitat (Rotting apple). LB, 20-26C. Sampled in Santeuil, France. More information about collection on the project's wiki: http://www.cembio.uni-kiel.de/. 16S rRNA primer: 27F/1492R. 16S rRNA sequence: ATGGAGAGTTTGATCCTGGCTCAGGATGAACGCTAGCGGGAGGCCTAACACATGCAAGCCGAGCGGTAGAGATCTTTCGGGATCTTGAGAGCGGCGTACGGGTGCGGAACACGTGTGCAACCTGCCTTTATCAGGGGGATAGCCTTTCGAAAGGAAGATTAATACCCCATAATATATTGAATGGCATCATTTGATATTGAAAACTCCGGTGGATAGAGATGGGCACGCGCAAGATTAGATAGTTGGTAGGGTAACGGCCTACCAAGTCAGTGATCTTTAGGGGGCCTGAGAGGGTGATCCCCCACACTGGTACTGAGACACGGACCAGACTCCTACGGGAGGCAGCAGTGAGGAATATTGGACAATGGGTGAGAGCCTGATCCAGCCATCCCGCGTGAAGGACGACGGCCCTATGGGTTGTAAACTTCTTTTGTATAGGGATAAACCTTTCCACGTGTGGAAAGCTGAAGGTACTATACGAATAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTAGGCGGATCTGTAAGTCAGTGGTGAAATCTCATAGCTTAACTATGAAACTGCCATTGATACTGCAGGTCTTGAGTAAAGTAGAAGTGGCTGGAATAAGTAGTGTAGCGGTGAAATGCATAGATATTACTTAGAACACCAATTGCGAAGGCAGGTCACTATGTTTTAACTGACGCTGATGGACGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGCTAACTCGTTTTTGGGTCTTCGGATTCAGAGACTAAGCGAAAGTGATAAGTTAGCCACCTGGGGAGTACGTTCGCAAGAATGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGATTATGTGGTTTAATTCGATGATACGCGAGGAACCTTACCAAGGCTTAAATGGGAATTGACAGGTTTAGAAATAGACTTTTCTTCGGACAATTTTCAAGGTGCTGCATGGTTGTCGTCAGCTCGTGCCGTGAGGTGTTAGGTTAAGTCCTGCAACGAGCGCAACCCCTGTCACTAGTTGCCATCATTCAGTTGGGGACTCTAGTGAGACTGCCTACGCAAGTAGAGAGGAAGGTGGGGATGACGTCAAATCATCACGGCCCTTACGCCTTGGGCCACACACGTAATACAATGGCCGGTACAGAGGGCAGCTACCTAGCGATAGGATGCGAATCTCGAAAGCCGGTCTCAGTTCGGATTGGAGTCTGCAACTCGACTCTATGAAGCTGGAATCGCTAGTAATCGCATATCAGCCATGATGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCAAGCCATGGAAGTTTGGGGTACCTGAAGTCGGTGACCGTAACAGGAGCTGCCTAGGGTAAAACAAGTAACTAGGGCTAAGTCGTAACAAGGTAGCCGTACCGGAAGGTGCGGCTGGAACATCTCATT Mutagen: Outcrossed: x0 Made by: Wild isolate Received: 07/17/19 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JUb66 Species: Lelliottia amnigena Genotype: Lelliottia amnigena Description: [NOTE: (04/06/2023) A user has reported that they have performed whole-genome sequencing and found this strain is actually Lelliottia nimipressuralis, not Lelliottia amnigena.] Bacteria. CeMbio Collection. Natural isolate from C. elegans natural habitat (Rotting apple). LB, 20-26C. Sampled in Santeuil, France. More information about collection on the project's wiki: http://www.cembio.uni-kiel.de/. 16S rRNA primer: 27F/1492R. 16S rRNA sequence: TTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGA GCGGTAGCACAGAGAGCTTGCTCTCGGGTGACGAGCGGCGGACGGGTGAGTAATGTCTG GGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGC AAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCT AGTAGGTGGGGTAATGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAG CCACACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGC ACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAA AGTACTTTCAGCGAGGAGGAAGGCATTGTGGTTAATAACCGCAGTGATTGACGTTACTCGCA GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA ATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTCTGTCAAGTCGGATGTGAAATCCCC GGGCTCAACCTGGGAACTGCATTCGAAACTGGCAGGCTAGAGTCTTGTAGAGGGGGGTAGA ATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCC CCCTGGACAAAGACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCC TGGTAGTCCACGCCGTAAACGATGTCGACTTGGAGGTTGTTCCCTTGAGGAGTGGCTTCC GGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGA ATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGATGCAACGCGAAGAAC CTTACCTACTCTTGACATCCAGAGAACTTAGCAGAGATGCTTTGGTGCCTTCGGGAACTCTG AGACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTTGTGAAATGTTGGGTTAAGTCCCGCAA CGAGCGCAACCCTTATCCTTTGTTGCCAGCGGTTCGGCCGGGAACTCAAAGGAGACTGCC AGTGATAAACTGGAGGAAGGTGGGGATGACGTCAAGTCATCATGGCCCTTACGAGTAGGGCT ACACACGTGCTACAATGGCATATACAAAGAGAAGCGACCTCGCGAGAGCAAGCGGACCTCA CAAAGTATGTCGTAGTCCGGATCGGAGTCTGCAACTCGACTCCGTGAAGTCGGAATCGCTA GTAATCGTAGATCAGAATGCTACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCA CACCATGGGAGTGGGTTGCAAAAGAAGTAGGTAGCTTAACCTTCGGGAGGGCGCTTACCAC TTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGA TCACCTCCTT GenBank: BioSample SAMN10361116 Mutagen: Outcrossed: x0 Made by: Wild isolate Received: 07/17/19 Inst Biology of the Ecole Normale Superieure, Paris, France ----------------------------------------------------------------------------- Strain: JUP1 Species: Caenorhabditis elegans Genotype: oxSi120 II; him-8(e1489) IV. Description: oxSi120 [peel-1p::tagRFP::MSP-142 3'utr+ unc-119(+)]. Him. Derived from EG5897 and CB1489; not known if is unc-119(ed3) is still present in background. Reference: Batchelder EL, et al. Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11429-34. Mutagen: Outcrossed: x Made by: Julie Plastino Received: 08/19/11 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: JV1 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; jrIs1. Description: jrIs1 [rpl-17p::HyPer + unc-119(+)]. Stable transgene ubiquituously expressing the YFP-based hydrogen peroxide sensor HyPer under a ribosomal promoter. Reference: Back P, et al. Free Radic Biol Med. 2012 Mar 1;52(5):850-9. Mutagen: Outcrossed: x2 Made by: Back & Braeckman Received: 03/02/17 University of Gent, Gent, Belgium ----------------------------------------------------------------------------- Strain: JV2 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; jrIs2. Description: jrIs2 [rpl-17p::Grx1-roGFP2 + unc-119(+)]. Stable transgene ubiquituously expressing the roGFP2 sensor under a ribosomal promoter for in vivo estimation of GSSG/2GSH ratios. Reference: Back P, et al. Free Radic Biol Med. 2012 Mar 1;52(5):850-9. Mutagen: Outcrossed: x3 Made by: Back & Braeckman Received: 03/02/17 University of Gent, Gent, Belgium ----------------------------------------------------------------------------- Strain: JVR406 Species: C.elegans Genotype: jerEx30. Description: jerEx30 [ddr-2p::BiFC1 (EGFH1-LINK-SYN) + tph-1p::BIFC2 (SYN-EGFH2) + rol-6(su1006)]. Pick Rollers to maintain. a-Synuclein BiFC transfer strain is a model to investigate neuron-to-neuron alpha-syn transfer. Reference: Tyson T, et al. Sci Rep. 2017 Aug 8;7(1):7506. Mutagen: Outcrossed: x0 Made by: Megan Senchuk, Trevor Tyson Received: 11/24/20 Van Andel Research Institute, Grand Rapids, MI ----------------------------------------------------------------------------- Strain: JW101 Species: Caenorhabditis elegans Genotype: unc-73(e936) I; sup-39(je5) II. Description: WT movement. Various degrees of embryonic lethality and Egl. About 1% Roll after L4. Mutagen: EMS Outcrossed: x1 Made by: Received: 02/07/97 Fuji ImmunoPharmaceuticals, Lexington, MA ----------------------------------------------------------------------------- Strain: JW102 Species: Caenorhabditis elegans Genotype: sup-39(je5) II. Description: je5 is a dominant suppressor of unc-73(e936). WT movement. Various degrees of embryonic lethality and Egl. About 1% Roller after L4. Mutagen: EMS Outcrossed: x Made by: Received: 02/07/97 Fuji ImmunoPharmaceuticals, Lexington, MA ----------------------------------------------------------------------------- Strain: JW103 Species: Caenorhabditis elegans Genotype: sup-39(je6) II. Description: je6 is a dominant suppressor of unc-73(e936). WT movement. Various degrees of embryonic lethality and Egl. About 1% Roller after L4. Mutagen: EMS Outcrossed: x Made by: Received: 02/07/97 Fuji ImmunoPharmaceuticals, Lexington, MA ----------------------------------------------------------------------------- Strain: JW104 Species: Caenorhabditis elegans Genotype: dpy-10(e128) sup-39(je5) unc-4(e120) II. Description: Dpy Unc-4 phenotype. Sick. Mutagen: Outcrossed: x Made by: Received: 02/07/97 Fuji ImmunoPharmaceuticals, Lexington, MA ----------------------------------------------------------------------------- Strain: JW105 Species: Caenorhabditis elegans Genotype: dpy-10(e128) sup-39(je5) unc-4(e120)/unc-104(e1265) II. Description: Heterozygotes are WT and segregate WT, Dpy Unc-4, and Unc-104s. Mutagen: Outcrossed: x Made by: Received: 02/07/97 Fuji ImmunoPharmaceuticals, Lexington, MA ----------------------------------------------------------------------------- Strain: JW106 Species: Caenorhabditis elegans Genotype: sup-39(je5) II; syr-1(je11) ?. Description: Synthetic Roller after L4 stage (85% penetrance). je5 is dominant and je11 is recessive. See 1996 East Coast Worm Meeting Abstract #80. Mutagen: Tc1 Outcrossed: x Made by: Received: 02/07/97 Fuji ImmunoPharmaceuticals, Lexington, MA ----------------------------------------------------------------------------- Strain: JW29 Species: Caenorhabditis elegans Genotype: jeIs1I. Description: jeIs1 [mec-7(+)::lacZ + rol-6(su1006)]. Rollers. Males will mate. Mutagen: transformation-gamma rays Outcrossed: x Made by: Jeff Way Received: 07/23/92 Merck Research Laboratories, Rahway, NJ ----------------------------------------------------------------------------- Strain: JY190 Species: Caenorhabditis elegans Genotype: osm-9(yz6) IV. Description: Downregulation of tph-1::GFP expression in the ADF neurons. CORRECTION: The yz6 allele had been erroneously listed as y26 in our database. Mutagen: EMS Outcrossed: x6 Made by: Ji Ying Sze Received: 03/25/05 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: JY243 Species: Caenorhabditis elegans Genotype: ocr-2(yz5) IV. Description: Downregulation of tph-1::GFP expression in the ADF neurons. Mutagen: EMS Outcrossed: x6 Made by: Ji Ying Sze Received: 03/25/05 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: JY359 Species: Caenorhabditis elegans Genotype: lim-4(yz12) X. Description: Down regulation of tph-1::GFP expression in the ADF neurons. Mutagen: EMS Outcrossed: x2 Made by: Ji Ying Sze Received: 08/26/03 Albert Einstein College of Medicine, Bronx, NY ----------------------------------------------------------------------------- Strain: JZ500 Species: Caenorhabditis elegans Genotype: pyIs500. Description: pyIs500 [ofm-1p::GFP + odr-1p::DsRed + odr-3p::GFP::egl-4]. Reference: O'Halloran DM, et al. PLoS Genet. 2009 Dec;5(12):e1000761. Lee JL et al. Proc Natl Acad Sci USA. 2010 Mar 30;107(13):6016-21. Mutagen: UV/TMP Outcrossed: x10 Made by: Lee & Eastman-Anders Received: 06/12/12 University of California, San Francisco, CA ----------------------------------------------------------------------------- Strain: KA41 Species: Caenorhabditis elegans Genotype: lkIs1 II. Description: lkIs1 [elp-1::GFP + lin-15(+)] II. Relatively low levels of GFP expression as compared to KA42. Superficially wild-type. Maintain under normal conditions. Reference: Hueston JL, et al. BMC Dev Biol. 2008 Nov 17;8:110. Mutagen: UV/TMP Outcrossed: x10 Made by: Andrew Box Received: 02/22/11 University of Kansas, Lawrence, KS ----------------------------------------------------------------------------- Strain: KA42 Species: Caenorhabditis elegans Genotype: lkIs2 IV. Description: lkIs2 [elp-1::GFP + lin-15(+)] IV. Relatively high levels of GFP expression as compared to KA41. Superficially wild-type. Maintain under normal conditions. Reference: Hueston JL, et al. BMC Dev Biol. 2008 Nov 17;8:110. Mutagen: UV/TMP Outcrossed: x10 Made by: Andrew Box Received: 02/22/11 University of Kansas, Lawrence, KS ----------------------------------------------------------------------------- Strain: KA6 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) X; lkEx1. Description: lkEx1 [elp-1::GFP + lin-15(+)]. Animals carrying the aray are superficially wild-type. Pick wild-type to maintain. Maintain under normal conditions. Reference: Hueston JL, et al. BMC Dev Biol. 2008 Nov 17;8:110. Mutagen: Outcrossed: x10 Made by: Jennifer Hueston Received: 02/22/11 University of Kansas, Lawrence, KS ----------------------------------------------------------------------------- Strain: KAB111 Species: C. elegans Genotype: louIs7. Description: louIs7 [ges-1p::sqst-1::mCherry::GFP::unc-54 3'UTR]. Tandemly-tagged p62/sequestosome marker expressed in the gut can be used to monitor autophagic activity. Reference: Villalobos TV, et al. Nat Aging. 2023 Sep;3(9):1091-1106. PMID: 37580394. Mutagen: Outcrossed: x5 Made by: Adam Bohnert Received: 07/24/24 Louisiana State University ----------------------------------------------------------------------------- Strain: KAB122 Species: C. elegans Genotype: louIs8. Description: louIs8 [ges-1p::nuc-1::mCherry::unc-54 3'UTR]. mCherry-tagged lysosomal nuclease NUC-1 expression can be used to monitor lysosomes in the gut. Reference: Villalobos TV, et al. Nat Aging. 2023 Sep;3(9):1091-1106. PMID: 37580394. Mutagen: Outcrossed: x5 Made by: Adam Bohnert Received: 07/24/24 Louisiana State University ----------------------------------------------------------------------------- Strain: KAB157 Species: C. elegans Genotype: louIs10. Description: louIs10 [myo-3p::sqst-1::mCherry::GFP::unc-54 3'UTR]. Tandemly-tagged p62/sequestosome marker expressed in the muscle can be used to monitor autophagic activity. Reference: Villalobos TV, et al. Nat Aging. 2023 Sep;3(9):1091-1106. PMID: 37580394. Mutagen: Outcrossed: x5 Made by: Adam Bohnert Received: 07/24/24 Louisiana State University ----------------------------------------------------------------------------- Strain: KAB34 Species: C. elegans Genotype: louIs2. Description: louIs2 [ges-1p::mCherry::GFP::SKL::unc-54 3' UTR]. Expresses a mCherry::GFP::serine-lysine-leucine peroxisome signal sequence (SKL) fluorescent pexophagy reporter in the C. elegans intestine. Generated in N2 background. Reference: Dolese DA, et al. Autophagy. 2022 Jul;18(7):1522-1533. https://doi.org/10.1080/15548627.2021.1990647 PMID: 34689720 Mutagen: integrated by UV Outcrossed: x6 Made by: Adam Bohnert Received: 01/25/23 Louisiana State University ----------------------------------------------------------------------------- Strain: KAB72 Species: C. elegans Genotype: spin-2(ok2121) IV; spin-1(ok2087) V; spin-3(ok2286) X. Description: Triple mutant of the three spinster paralogs expressed exclusively in somatic tissues. Reference: Villalobos TV, et al. Nat Aging. 2023 Sep;3(9):1091-1106. PMID: 37580394. Mutagen: Outcrossed: x0 Made by: Adam Bohnert Received: 07/24/24 Louisiana State University ----------------------------------------------------------------------------- Strain: KAE10 Species: Caenorhabditis elegans Genotype: seaSi40 I; unc-119(ed3) III. Description: seaSi40 [(pCFJ448) (eft-3p::fmo-2 + H2B::GFP) + Cbr-unc-119(+)] I. Higher level of FMO-2 over-expression compared to KAE9. Improved healthspan, stress resistance and longevity. Reference: Leiser SF, et al. Science 350.6266 (2015): 1375-8. Mutagen: MosSCI Outcrossed: x6 Made by: Scott Leiser Received: 01/22/16 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: KAE112 Species: C. elegans Genotype: seaIs201. Description: seaIs201 [myo-3p::human tau (0N4R;V337M)::unc-54 3'UTR + vha-6p::mCherry::unc-54 3'UTR]. Reduced crawling speed, reduced brood size, shortened lifespan, slow development, early paralysis. Human tau transgene is expressed in body wall muscles, producing strong phenotypes suitable for screening and is sensitive to knockdown by feeding RNAi. Generated in N2 background. Mutagen: Outcrossed: x6 Made by: Joshua Russell Received: 10/12/21 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: KAE12 Species: Caenorhabditis elegans Genotype: seaSi182 II; unc-119(ed3) III. Description: seaSi182 [(pCFJ150) (vha-6p::fmo-2 + H2B::GFP) + Cbr-unc-119(+)] II. Over-expression of FMO-2 in the intestine. Long-lived. Reference: Leiser SF, et al. Science 350.6266 (2015): 1375-8. Mutagen: MosSCI Outcrossed: x4 Made by: Hillary Miller Received: 01/22/16 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: KAE28 Species: Caenorhabditis elegans Genotype: unc-119(ed3) III; seaEx13. Description: seaEx13 [fmo-2p::GFP + unc-119(+)]. Pick non-Unc to maintain. Reference: Leiser SF, et al. Science 350.6266 (2015): 1375-8. Mutagen: Outcrossed: x0 Made by: Hillary Miller Received: 01/22/16 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: KAE9 Species: Caenorhabditis elegans Genotype: seaSi39 I; unc-119(ed3) III. Description: seaSi39 [(pCFJ448) (eft-3p::fmo-2 + H2B::GFP) + Cbr-unc-119(+)] I. Lower level of FMO-2 over-expression compared to KAE10. Improved healthspan, stress resistance and longevity. Reference: Leiser SF, et al. Science 350.6266 (2015): 1375-8. Mutagen: MosSCI Outcrossed: x4 Made by: Scott Leiser Received: 01/22/16 University of Washington, Seattle, WA ----------------------------------------------------------------------------- Strain: KB10 Species: Caenorhabditis elegans Genotype: mir-67(n4899) III. Description: MT15982 mir-67(n4899) was outcrossed 6x to produce this strain. Deletion breakpoints are:GGGTGCCTAATGCAAA / AGTACACATTTATGAAT...GCGAGTTTAAAGCAACG / AGTAGCAGAAGGACCA.Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: UV/TMP Outcrossed: x6 Made by: Horvitz Lab Received: 04/29/10 University of Missouri, Columbia ----------------------------------------------------------------------------- Strain: KB11 Species: Caenorhabditis elegans Genotype: mir-83(n4638) IV. Description: MT15501 mir-83(n4638) was outcrossed 6x to produce this strain. Deletion breakpoints are:GTTGAGAATTCCTGTTGCAAT / TAAAACTGAAATTTCGATCTA...TTTTTAGAATTGAGAGCA / ACGAAAGAACAAAATAAGAGA.Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Mutagen: UV/TMP Outcrossed: x6 Made by: Horvitz Lab Received: 04/29/10 University of Missouri, Columbia ----------------------------------------------------------------------------- Strain: KB12 Species: Caenorhabditis elegans Genotype: mir-67(n4899) III; mir-83(n4638) IV. Description: Combination mutant made with 6x outcrossed lines KB10 mir-67(n4899) and KB11 mir-83(n4638). Mutagen: UV/TMP Outcrossed: x6 Made by: Bennett Lab Received: 04/29/10 University of Missouri, Columbia ----------------------------------------------------------------------------- Strain: KB2 Species: Caenorhabditis elegans Genotype: glh-2(um2) I. Description: Mutagen: UV/TMP Outcrossed: x>6 Made by: Kathleen Kuznicki Received: 10/09/07 University of Missouri, Columbia ----------------------------------------------------------------------------- Strain: KB3 Species: Caenorhabditis elegans Genotype: kgb-1(um3) IV. Description: Temperature sensitive sterile at 26C, with EMO oocytes. Grows at 15C or 20C. Mutagen: UV/Psoralen Outcrossed: x6 Made by: Karen Bennett Received: 01/19/06 University of Missouri, Columbia ----------------------------------------------------------------------------- Strain: KB4 Species: Caenorhabditis elegans Genotype: glh-4(gk225) glh-1(ok439) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Description: Pick GFP+ to maintain balanced stock. Heterozygotes are superficially wild-type GFP+ and segregate wild-type GFP+ (heterozygotes), arrested hT2 aneuploids, and non-GFP glh-4 glh-1 homozygotes (sterile; incompletely penetrant). NOTE (K. Bennett, 2012): KB4 strain is only 63% sterile at 20C and 92% sterile at 26C (Spike et al., Genetics 2008 178:1973). glh-1(ok439) is not a null allele. Mutagen: UV/TMP Outcrossed: x>6 Made by: Chris Yee Received: 10/23/12 University of Missouri, Columbia ----------------------------------------------------------------------------- Strain: KB6 Species: Caenorhabditis elegans Genotype: rle-1(cxTi510) III. Description: Slowed development. Increased life span. Decreased embyronic viability. Decreased brood sizes. Mutagen: Tc5 Outcrossed: x6 Made by: L. Segalat Received: 05/04/09 University of Missouri, Columbia ----------------------------------------------------------------------------- Strain: KB7 Species: Caenorhabditis elegans Genotype: kgb-1(um3) kgb-2(km16) IV. Description: Deletion alleles. Can be maintained at 20C. Sterile at 26C. Can use PCR with the following primer to determine whether both deletions are present. (5'end)5'GGTCTACCAGAGTTTGTGCGCAATC3' (3'end)5'GATAGCCTTGCACTTCGTTG3'. PCR products from wild type show two bands; kgb-1 gene shows a smaller band; kgb-2 gene shows a bigger band.The double mutant should have no PCR product. [NOTE: The 5' primer sequence was corrected 03/28/12 (K. Bennett)] Mutagen: UV/TMP Outcrossed: x6 Made by: April Orsborn Received: 10/18/07 University of Missouri, Columbia ----------------------------------------------------------------------------- Strain: KC322 Species: Caenorhabditis remanei Genotype: C. remanei (wx23) A. Description: Caenorhabditis remanei mutant. Small (non-Mab). Male-female strain. Mutagen: EMS Outcrossed: x2 Made by: King Chow Received: 02/17/04 Hong Kong U of Sci & Tech, Clear Water Bay, Kowloon, Hong Kong ----------------------------------------------------------------------------- Strain: KC421 Species: Caenorhabditis remanei Genotype: C. remanei (wx32) A. Description: Caenorhabditis remanei mutant. Unc. Male-female strain. Mutagen: EMS Outcrossed: x2 Made by: King Chow Received: 02/17/04 Hong Kong U of Sci & Tech, Clear Water Bay, Kowloon, Hong Kong ----------------------------------------------------------------------------- Strain: KC422 Species: Caenorhabditis remanei Genotype: C. remanei (wx36) A. Description: Caenorhabditis remanei mutant. Dumpy. Male-female strain. Mutagen: EMS Outcrossed: x2 Made by: King Chow Received: 02/17/04 Hong Kong U of Sci & Tech, Clear Water Bay, Kowloon, Hong Kong ----------------------------------------------------------------------------- Strain: KC423 Species: Caenorhabditis remanei Genotype: C. remanei (wx42) A. Description: Caenorhabditis remanei mutant. Uncoordinated (coiler). Male-female strain. Mutagen: EMS Outcrossed: x2 Made by: King Chow Received: 02/17/04 Hong Kong U of Sci & Tech, Clear Water Bay, Kowloon, Hong Kong ----------------------------------------------------------------------------- Strain: KC427 Species: Caenorhabditis remanei Genotype: C. remanei (wx29) A. Description: Caenorhabditis remanei mutant. Unc. Male-female strain. Mutagen: EMS Outcrossed: x2 Made by: King Chow Received: 02/17/04 Hong Kong U of Sci & Tech, Clear Water Bay, Kowloon, Hong Kong ----------------------------------------------------------------------------- Strain: KC431 Species: Caenorhabditis remanei Genotype: C. remanei (wx34) A. Description: Caenorhabditis remanei mutant. Small (non-Mab)/Dumpy. Male-female strain. Mutagen: EMS Outcrossed: x2 Made by: King Chow Received: 02/17/04 Hong Kong U of Sci & Tech, Clear Water Bay, Kowloon, Hong Kong ----------------------------------------------------------------------------- Strain: KC435 Species: Caenorhabditis remanei Genotype: C. remanei (wx30) X. Description: Caenorhabditis remanei mutant. Small (non-Mab)/Dumpy. Male-female strain. Mutagen: EMS Outcrossed: x2 Made by: King Chow Received: 02/17/04 Hong Kong U of Sci & Tech, Clear Water Bay, Kowloon, Hong Kong ----------------------------------------------------------------------------- Strain: KC436 Species: Caenorhabditis remanei Genotype: C. remanei (wx33) X. Description: Caenorhabditis remanei mutant. Dumpy. Male-female strain. Mutagen: EMS Outcrossed: x2 Made by: King Chow Received: 02/17/04 Hong Kong U of Sci & Tech, Clear Water Bay, Kowloon, Hong Kong ----------------------------------------------------------------------------- Strain: KC440 Species: Caenorhabditis remanei Genotype: C. remanei (wx49) A. Description: Caenorhabditis remanei mutant. Dumpy. Slightly Uncoordinated. Male-female strain. Mutagen: EMS Outcrossed: x2 Made by: King Chow Received: 02/17/04 Hong Kong U of Sci & Tech, Clear Water Bay, Kowloon, Hong Kong ----------------------------------------------------------------------------- Strain: KC441 Species: Caenorhabditis remanei Genotype: C. remanei (wx51) A. Description: Caenorhabditis remanei mutant. Dumpy. Male-female strain. Mutagen: EMS Outcrossed: x2 Made by: Fan Chu Received: 02/17/04 Hong Kong U of Sci & Tech, Clear Water Bay, Kowloon, Hong Kong ----------------------------------------------------------------------------- Strain: KC442 Species: Caenorhabditis remanei Genotype: C. remanei (wx41) X. Description: Caenorhabditis remanei mutant. Dumpy. Male-female strain. Mutagen: EMS Outcrossed: x2 Made by: King Chow Received: 02/17/04 Hong Kong U of Sci & Tech, Clear Water Bay, Kowloon, Hong Kong ----------------------------------------------------------------------------- Strain: KC443 Species: Caenorhabditis remanei Genotype: C. remanei (wx50) A. Description: Caenorhabditis remanei mutant. Dumpy. Male-female strain. Mutagen: EMS Outcrossed: x2 Made by: Fan Chu Received: 02/17/04 Hong Kong U of Sci & Tech, Clear Water Bay, Kowloon, Hong Kong ----------------------------------------------------------------------------- Strain: KC467 Species: Caenorhabditis remanei Genotype: C. remanei (wx38) A. Description: Caenorhabditis remanei mutant. Unc (coiler). Male-female strain. Mutagen: EMS Outcrossed: x2 Made by: King Chow Received: 02/17/04 Hong Kong U of Sci & Tech, Clear Water Bay, Kowloon, Hong Kong ----------------------------------------------------------------------------- Strain: KC470 Species: Caenorhabditis remanei Genotype: C. remanei (wx53) A. Description: Male-female strain. Caenorhabditis remanei mutant. Dumpy. Mutagen: EMS Outcrossed: x3 Made by: YM Wong Received: 02/17/04 Hong Kong U of Sci & Tech, Clear Water Bay, Kowloon, Hong Kong ----------------------------------------------------------------------------- Strain: KC565 Species: Caenorhabditis elegans Genotype: sin-3(tm1276) I; him-5(e1490) V. Description: Mab. Pvl. Throws males. Mutagen: UV/TMP Outcrossed: x2 Made by: Sarah SW Choy Received: 07/16/07 Hong Kong U of Sci & Tech, Clear Water Bay, Kowloon, Hong Kong ----------------------------------------------------------------------------- Strain: KDK53250 Species: C. elegans Genotype: oskEx53250. Description: oskEx53250 [dat-1p::GCaMP6f + dat-1p::mCherry + lin-44p::mRFP + N2 genomic DNA cut with Pvu II (as a carrier)]. Pick animals with red fluorescence to maintain. GCaMP6f and mCherry expressed in dopaminergic neurons. Generated in N2 background. Reference: Tanimoto Y, et al. Sci Rep. 2016 May 19;6:26297. doi: 10.1038/srep26297. PMID: 27193056. Mutagen: Outcrossed: x0 Made by: Kotaro Kimura Received: 10/14/22 Nagoya City University, Nagoya, Japan ----------------------------------------------------------------------------- Strain: KG115 Species: Caenorhabditis elegans Genotype: lin-1(e1275) unc-33(e204)/ric-8(ok98) IV. Description: ok98/+ animals are slightly Egl-d and have a small vulval bump. ok98 homozygotes are straight and paralyzed and slow growing. About 2/3 eventually reach adulthood and are paralyzed and sterile (they produce few if any oocytes). Segregates Unc Muv(ts). Mutagen: Outcrossed: x3 Made by: Received: 02/23/05 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG1180 Species: Caenorhabditis elegans Genotype: lite-1(ce314) X. Description: Defective response to short wavelength light; response strongly reduced but not eliminated. All other characteristics seem wild type, including reponse to mechanosensory stimuli. Strong, probably null, allele. This mutation also blocks the coordinated light response of unc-31(e928) and egl-30(ad805). To identify lite-1 homozygous mutants when crossing into different backgrounds, use a fluorescence stereomicroscope with a GFP filter and zoom to the hightest magnification (60-100X) to distinguish Lite from non-Lite animals. This works best when the animals are mired in thicker parts of the food to slow their spontaneous locomotion but not their response to light. Scan animals around the edge of the food where it is thickest. Leave the lid of the plate off for a minute or so before starting to let the animals adjust to air currents. Mutagen: Outcrossed: x5 Made by: Received: 09/24/08 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG1278 Species: Caenorhabditis elegans Genotype: unc-73(ce362) I. Description: Slow growing. Sluggish. Egl-D. Mutagen: ENU Outcrossed: x Made by: Received: 11/08/07 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG1397 Species: Caenorhabditis elegans Genotype: unc-73(ev802) I. Description: Mutagen: Outcrossed: x Made by: Received: 11/08/07 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG2338 Species: Caenorhabditis elegans Genotype: unc-16(ce483) III. Description: Null or nearly null allele. Sluggish locomotion. Slightly short. Egl. Growth rate ~72% of wild-type. Reference: Edwards SL, et al. Genetics May 2013 194:143-161. (See Commentary in same issue 194:35-37). Mutagen: EMS Outcrossed: x5 Made by: Ken Miller Received: 04/29/13 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG2430 Species: C. elegans Genotype: ceIs56 X. Description: ceIs56 [unc-129p::ctns-1::mCherry + nlp-21p::Venus + ttx-3p::RFP]; Maps to X: 9.0 +/ 3.0 m.u. Expresses the lysosomal membrane marker CTNS-1 in a subset of 9 DA/DB cholinergic motor neurons in the ventral cord, and NLP-21::Venus in the same neurons as a marker for soluble DCV cargo and to help identify the boundaries of the somas and axons when imaging lysosomes. Reference: Edwards SL, et al. Genetics. 2015 Sep;201(1):91-116. Edwards SL, et al. Genetics. 2015 Sep;201(1):117-41. Mutagen: Gamma Rays Outcrossed: x2 Made by: Nicole Charlie Received: 05/02/18 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG2730 Species: Caenorhabditis elegans Genotype: clu-1(ok2). Description: Mild locomotory defects, sluggish response to physical stimuli, and reduced thrashing rate. Phenotype more severe than ok3. ok2 is a 1189 bp deletion (III:9519159-9520346) removing amino acids 314-693 and inserting a glutamic acid at the deletion site. Flanking sequence: GACCGACTTCCAACCAGTTACCCAG...ATGTTATGAAGTTCAATCCGGATTGTTTCTC ATCAAATGT. Mutagen: UV-Psoralen Outcrossed: x Made by: R Barstead/M Clarke Received: 03/16/11 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG2731 Species: Caenorhabditis elegans Genotype: clu-1(ok3). Description: Mild locomotory defects, sluggish response to physical stimuli, and reduced thrashing rate. Phenotype less severe than ok2. ok3 is a 1047 bp deletion (III:9518654-9519699) and 13 nucleotude insertion (producing two termination codons) resulting in a protein truncated at amino acid 693 plus an inserted glutamic acid. Flanking sequence: GCTCGAGGATGCTGCTCACAAACTGAAAATG...GAATAGTATCCGTGAATAGTATCCG TGAAGATTCTG. Mutagen: UV-Psoralen Outcrossed: x Made by: R Barstead/M Clarke Received: 03/16/11 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG421 Species: Caenorhabditis elegans Genotype: gsa-1(ce81) I. Description: Coordinated hyperactive locomotion. Hypersensitive to mechanical stimuli. Most mature adults have vulval bump. Mature animals are slightly short and egl-d. Terminal phenotype is usually bag of worms. Population tends to severely crash within 2-3 weeks of starvation. Dominant, gain-of-function allele. Heterozygotes are difficult to distinguish from homozygotes. Mutation is R182C. This same mutation causes constitutive Gs activity in vertebrates. Sequence in WT: CCT TCG GTG TCG. Sequence in ce81: CCT TCG GTG TTG. Mutagen: Outcrossed: x5 Made by: Received: 02/23/05 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG4247 Species: C. elegans Genotype: ceIs201 I. Description: ceIs201 [unc-17p::ins-22::Venus + unc-17p::RFP + unc-17p::ssmCherry + myo-2p::RFP]; integration site maps to I:0.77. Expresses INS-22::Venus to mark Dense Core Vesicles in the cholinergic nervous system, including the ventral cord cholinergic motor neurons. Also expresses mCherry in the same neurons to help identify the boundaries of the somas, axons, and dendrites. ssmCherry is mCherry with a secretion signal on its N-terminus, which is constitutively secreted and taken up by coelomoctyes in the pseuodcoelomic space, making it a useful marker for those coelomocytes. The INS-22::Venus also gets secreted by DCVs and taken up by coelomocytes. The myo-2::RFP is a co-tranformation marker that lights up the pharyngeal muscle cells and is useful for crossing the integrant into various mutant backgrounds. Reference: Hoover CM, et al. Genetics. 2014 Mar;196(3):745-65. Mutagen: Gamma Rays Outcrossed: x4 Made by: Ken Miller Received: 05/02/18 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG4386 Species: Caenorhabditis elegans Genotype: unc-104(ce782) II. Description: Temperature sensitive. Maintain at 15C. Animals grown at 14C take 23% longer than wild-type to reach adulthood, exhibit 0% larval arrest, locomotion rates that are ~40% of wild type, and synaptic vesicle densities at synapses that are ~60% of wild type. Animals grown at 20C take 80% longer than wild type to reach adulthood, exhibit locomotion rates that are ~3% of wild type, and synaptic vesicle densities at synapses that are ~2% of wild type. Animals grown at 25C exhibit >90% larval arrest. When shifting late larval stage and adult animals from 14C to 25C, it takes 8-12 hours to see effects on locomotion and SV density. Reference: Edwards SL, et el. Genetics. 2015 Sept 201(1): 91-116. Mutagen: EMS Outcrossed: x2 Made by: Ken Miller Received: 09/15/15 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG4400 Species: C. elegans Genotype: sad-1(ce749) X. Description: Superficially wild-type on plates. Shows defects in the transport and capture of dense core vesicles and synaptic vesicles in cholinergic motor neurons. Suppresses organelle accumulation (lysosomes, early endosomes, Golgi) in axons in an unc-16(-) background. Reference: Edwards SL, et al. Genetics. 2015 Sep;201(1):91-116. Edwards SL, et al. Genetics. 2015 Sep;201(1):117-41. Mutagen: EMS Outcrossed: x2 Made by: Ken Miller Received: 05/02/18 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG4671 Species: C. elegans Genotype: ceIs259 IV. Description: ceIs259 [unc-129p::RFP::syn-13 + unc-129p::Venus + ttx-3p::RFP]; Inserted at center of LG IV. Expresses RFP::SYN-13 to mark early endosomes in a set of 9 DA/DB cholinergic motor neurons in the ventral nerve cord. unc-129::Venus fills the neuron and thus is useful for identifying regions and can also be used as a control for effects on expression of the transgene. Reference: Edwards SL, et al. Genetics. 2015 Sep;201(1):91-116. Edwards SL, et al. (manuscript in revision). "Sentryn Acts with a Subset of Active Zone Proteins in the Guided Transport and Capture of Synaptic Vesicles in Caenorhabditis elegans." Mutagen: Gamma Rays Outcrossed: x2 Made by: Stacey Edwards Received: 05/02/18 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG4687 Species: C. elegans Genotype: ceIs269 I. Description: ceIs269 [unc-129p::tomm-20::Venus + unc-129p::mCherry + ttx-3p::RFP]; Integration in Chromosome I (near genetic position -3.20 or 1.14). The ttx-3 marker is quite faint in this strain especially in larvae. The tomm-20::Venus construct was injected at 0.5 ng/ ul in the strain used to make this integrant, so virtually all of the visible tomm-20::Venus signal s associated with mitochondria with essentially no background. The unc-129p::mCherry marker is expressed at a very low level that are detectable only with a sensitive camera (and often not by eye at 1000X). In strains lacking mitochondria in the dorsal cord, use the camera to focus on the mCherry in the dorsal cord, then acquire in the YFP channel. Mutagen: Gamma Rays Outcrossed: x2 Made by: Logan Morrison Received: 05/02/18 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG4731 Species: C. elegans Genotype: unc-116(ce815[LoxP+sup-1(e995)+LoxP]) III. Description: Lox P sites in 3' UTR and 4th intron flank kinesin motor domain sequences; sup-1(e995) mini-gene inserted in second intron. Appears wild-type on plates and in quantitative locomotion assays. Can be used to conditionally delete gene sequences encoding the conventional kinesin motor domain in a tissue specific manner by driving expression of Cre recombinase in the tissue of interest. Reference: Stec N, et al. (Submitted). An Intron Compatible Marker for Long Distance CRISPR Mediated Gene Editing in Caenorhabditis elegans. Mutagen: Outcrossed: x2 Made by: Stacey Edwards Received: 05/02/18 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG4995 Species: C. elegans Genotype: rimb-1(ce828) III. Description: Superficially wild type on plates. Slight (<10%), but significant, expansion of synaptic vesicles from the synaptic region of the DA9 motor neuron into the flanking asynaptic regions. Synaptic vesicles also showed a slight but significant accumulation in rimb-1 mutant dendrites in the DA9 motor neuron (192 +/- 32% compared to wild type; N=15; P=.015). The sequence GC TAG C TAA A TGA (3 successive stop codons in different reading frames) was inserted after codon 16 of the rimb-1 gene; described in Edwards SL, et al. (manuscript in revision). "Sentryn Acts with a Subset of Active Zone Proteins in the Guided Transport and Capture of Synaptic Vesicles in Caenorhabditis elegans." Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Stacey Edwards Received: 05/02/18 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG5082 Species: C. elegans Genotype: ceIs308. Description: ceIs308 [mig-13p::ins-22::Emerald + mig-13p::mCherry + odr-1p::RFP]; Linked to IVC (ceP86; 3.37): 0/17 recombinants. Expresses INS-22::Emerald to mark Dense Core Vesicles in the the DA9 and VA12 cholinergic motor neurons, and also mCherry in the same neurons to help identify the boundaries of the somas, axons, and dendrites. Useful for visualizing Dense Core Vesicles in a single, well-segregated neuron in living animals. When picking homozygotes from crosses with other strains, focus on the brightness of the RFP puncta in the cord. Autofluorescence in the worm body can make it difficult to gauge differences in brightness of the odr-1::RFP marker. Reference: Edwards SL, et al. (manuscript in revision). "Sentryn Acts with a Subset of Active Zone Proteins in the Guided Transport and Capture of Synaptic Vesicles in Caenorhabditis elegans." Mutagen: Gamma Rays Outcrossed: x2 Made by: Stacey Edwards Received: 05/02/18 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG5148 Species: C. elegans Genotype: unc-104(ce833[5xMyc::AID::unc-104+sup-1(e995)]) II. Description: The Auxin Inducible Degron (AID) flanked by a 5X Myc/spacer tag on left and a single spacer on the right is fused to the N-terminus of the unc-104 gene. Allows conditional degradation of UNC-104 protein when combined with tissue specific expression of TIR1 in the presence of 1 mM Auxin in plate media. Note: KG5148 does not express TIR1. On standard plates: wild type growth, appearance, and locomotion rate. For animals expressing a TIR1 transgene in the nervous system: Animals that hatch and develop on 1 mM Auxin plates generally remain tightly coiled near the location of hatching and exhibit slow growth (up to 7 days to reach adulthood, with 98% reaching adulthood by 7 days). Adults placed on Auxin abruptly lose about 75% of their locomotion function between 6 and 12 hours after plating. The remaining locomotion function is lost gradually between 12 and 52 hours. Reference: Stec N, et al. (Submitted). An Intron Compatible Marker for Long Distance CRISPR Mediated Gene Editing in Caenorhabditis elegans. Mutagen: N/A Outcrossed: x2 Made by: Stacey Edwards Received: 05/02/18 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG518 Species: Caenorhabditis elegans Genotype: acy-1(ce2) III. Description: About normal size and distribution on food. Hyperactive locomotion. Hypersensitive to stimuli. Most mature adults have slight vulval bump. Not obviously Egl-d or Egl-c. Dominant, gain-of-function allele. Mutation is P260S. Sequence in WT: CAGTCTGTGATG C CT. Sequence in ce2: CAGTCTGTGATG T CT. Mutagen: EMS Outcrossed: x5 Made by: Received: 02/23/05 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG522 Species: Caenorhabditis elegans Genotype: acy-1(md1756) III. Description: About normal size. Hyperactive locomotion. Hypersensitive to stimuli. Most mature adults have slight vulval bump. Not obviously Egl-d or Egl-c. Dominant, gain-of-function allele. Mutation is A337T. Sequence in WT: TAAATCT G CCGACG. Sequence in md1756: TAAATCT A CCGACG. Mutagen: EMS Outcrossed: x4 Made by: Received: 02/23/05 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG524 Species: Caenorhabditis elegans Genotype: gsa-1(ce94) I. Description: Short. Relatively constant motion (coordinated hyperactivity). Adults quickly become Egl-d and most have vulval bump. Hypersensitive to stimuli. Loopy backing, but does not like to back, especially after repeated stimuli. Terminal phenotype is usually bag of worms. Population tends to severely crash after 2-3 weeks of starvation. Dominant, gain-of-function allele. Heterozygotes are also hyperactive and difficult to tell apart from homozygotes. Mutation is G45R. Sequence in WT: GGC GCC G GA GAG AGC. Sequence in ce94: GGC GCC A GA GAG AGC. Mutagen: Outcrossed: x5 Made by: Received: 02/23/05 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG532 Species: Caenorhabditis elegans Genotype: kin-2(ce179) X. Description: Adults generally short, although some can reach near normal length. Slow growth rate. Backing can be loopy, but does not like to back. Strongly hypersensitive to stimuli. Relatively constant spontaneous movement. Some clustering especially in response to stimuli. Mature adults have vulval bump. Most young adults are Egl-c and young embryos can be found on the plate. Some mature adults become moderately Egl-d. Recessive allele. Males are small and slow growing. Mutation is R92C, which is a conserved residue in the 10 amino acid inhibitory domain that normally functions to keep protein kinase A turned off in the absence of cAMP. Population tends to severely crash within 2-3 weeks of starvation. Mutagen: Outcrossed: x5 Made by: Received: 02/23/05 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG571 Species: Caenorhabditis elegans Genotype: eat-16(ce71) I. Description: Mutagen: Outcrossed: x6 Made by: Received: 02/23/05 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KG744 Species: Caenorhabditis elegans Genotype: pde-4(ce268) II. Description: Hyperactive locomotion and hypersensitive to stimuli such as plate dropping. Restores wild type levels of locomotion to paralyzed ric-8(md303) mutants. Most, but not all adults, appear significantly Egl-c. Some mature adults are thinner than normal. Aldicarb sensitivity, growth rate, pumping, length, and distribution on food are all similar to wild type. The ce268 mutation is a D448N change relative to PDE-4D isoform. It disrupts the catalytic domain by changing one of the four active site residues that together chelate an active site zinc atom. Inheritance is semi-dominant due to dominant negative side effects. Mutagen: EMS Outcrossed: x5 Made by: Ken Miller Received: 06/08/06 OMRF, Oklahoma City, OK ----------------------------------------------------------------------------- Strain: KH1125 Species: Caenorhabditis elegans Genotype: asd-1(yb978) III; ybIs733. Description: ybIs733 [myo-3::egl-15::BGAR + lin-15(+)]. GFP/RFP chimeric expression of egl-15::BGAR reporter in body wall muscles. Mutagen: EMS Outcrossed: x Made by: Hidehito Kuroyanagi Received: 01/16/07 University of the Ryukyus, Okinawa, Japan ----------------------------------------------------------------------------- Strain: KIR1 Species: Caenorhabditis elegans Genotype: smc-4(tm1868) III/qC1 [dpy-19(e1259) glp-1(q339) qIs26] (III). Description: qIs26 [lag-2::GFP + rol-6(su1006)]. Rollers. Homozygous sterile deletion allele tm1868 balanced by qC1 with rol and GFP markers. Segregates GFP + Roller heterozygotes, and non-rol non-GFP tm1868 homozygotes (sick, sterile, unc). qIs26 was integrated into qC1 and in the process made qC1 homozygous lethal. Pick Rol GFP+ and check for correct segregation of progeny to maintain. Reference: Csankovszki G, et al., Curr Biol. 2009 Jan 13;19(1):9-19. Mutagen: Outcrossed: x4 Made by: K. Spahl Received: 02/22/11 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: KIR2 Species: Caenorhabditis elegans Genotype: capg-2(tm1833) V/nT1 [qIs51] (IV;V). Description: Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP capg-2 homozygotes (sick, sterile, Unc). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. Reference: Csankovszki G, et al., Curr Biol. 2009 Jan 13;19(1):9-19. Mutagen: Outcrossed: x8 Made by: K. Collette Received: 02/22/11 University of Massachusetts, Worcester, MA ----------------------------------------------------------------------------- Strain: KJ216 Species: Caenorhabditis elegans Genotype: crt-1(jh101) V. Description: Mutagen: UV/TMP Outcrossed: x6 Made by: BJ Park Received: 02/12/03 Hanyang University, Seoul, Korea ----------------------------------------------------------------------------- Strain: KJ300 Species: Caenorhabditis elegans Genotype: cnb-1(jh103) V. Description: Decreased brood size. Small body size. Slow growing. Resistant to 5HT serotonin. Mutagen: UV/TMP Outcrossed: x>6 Made by: J. Lee Received: 02/12/03 Hanyang University, Seoul, Korea ----------------------------------------------------------------------------- Strain: KJ306 Species: Caenorhabditis elegans Genotype: tax-6(jh107) IV. Description: Gain-of-function mutant. Hypersensitive to serotonin. Mutagen: UV/TMP Outcrossed: x6 Made by: Jungsoo Lee Received: 12/08/04 Hanyang University, Seoul, Korea ----------------------------------------------------------------------------- Strain: KJ355 Species: Caenorhabditis elegans Genotype: csq-1(jh109) X. Description: Sensitive to high and low calcium conditions. Mutagen: UV/TMP Outcrossed: x6 Made by: Jonghoon Cho Received: 06/25/07 Hanyang University, Seoul, Korea ----------------------------------------------------------------------------- Strain: KJ412 Species: Caenorhabditis elegans Genotype: trx-1(jh127) II. Description: Mutagen: UV/TMP Outcrossed: x6 Made by: Changhoon Ji Received: 06/25/07 Hanyang University, Seoul, Korea ----------------------------------------------------------------------------- Strain: KJ461 Species: Caenorhabditis elegans Genotype: cng-1(jh111) V. Description: Mutagen: TMP Outcrossed: x>6 Made by: Received: 12/29/08 Brandeis University ----------------------------------------------------------------------------- Strain: KJ462 Species: Caenorhabditis elegans Genotype: cng-3(jh113) IV. Description: Thermotolerant. Mutagen: EMS Outcrossed: x6 Made by: Suk-Woo Cho Received: 03/03/05 Hanyang University, Seoul, Korea ----------------------------------------------------------------------------- Strain: KJ487 Species: Caenorhabditis elegans Genotype: vha-8(jh135)/bli-6(sc16) egl-19(ad695) unc-24(e318) IV. Description: Heterozygotes are WT and segregate WT, Bli Unc, and dead larva. vha-8(jh135) homozygotes are larval lethal. Mutagen: UV/TMP Outcrossed: x6 Made by: YJ Ji & J Ahnn Received: 09/20/07 Hanyang University, Seoul, Korea ----------------------------------------------------------------------------- Strain: KJ550 Species: Caenorhabditis elegans Genotype: aco-1(jh131) X. Description: Mutagen: UV/TMP Outcrossed: x6 Made by: Youngil Kim Received: 06/25/07 Hanyang University, Seoul, Korea ----------------------------------------------------------------------------- Strain: KJ5559 Species: Caenorhabditis elegans Genotype: tax-4(p678) III; cng-1(jh111) V. Description: Mutagen: Outcrossed: x Made by: Received: 12/29/08 Brandeis University ----------------------------------------------------------------------------- Strain: KJ5560 Species: Caenorhabditis elegans Genotype: cng-3(jh113) IV; cng-1(jh111) V. Description: Mutagen: Outcrossed: x Made by: Received: 12/29/08 Brandeis University ----------------------------------------------------------------------------- Strain: KJ5561 Species: Caenorhabditis elegans Genotype: tax-4(p678) III; cng-3(jh113) IV. Description: Mutagen: Outcrossed: x Made by: Received: 12/29/08 Brandeis University ----------------------------------------------------------------------------- Strain: KJ5562 Species: Caenorhabditis elegans Genotype: tax-4(p678) III; cng-3(jh113) IV; cng-1(jh111) V. Description: Mutagen: Outcrossed: x Made by: Received: 12/29/08 Brandeis University ----------------------------------------------------------------------------- Strain: KK1105 Species: Caenorhabditis elegans Genotype: lgl-1(tm2616) X. Description: Mutagen: Outcrossed: x6 Made by: S Mitani/K Kemphues Received: 12/08/10 University of Minnesota, Minneapolis, MN ----------------------------------------------------------------------------- Strain: KK112 Species: Caenorhabditis elegans Genotype: mel-8(b309) unc-4(e120) sqt-1(sc13)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: mel-8 is a maternal effect lethal. WT heterozygotes (sc13 recessive) segregate WT, MelUncSqt (sick and small), mnC1 dpy-10 unc-52 homozygotes (paralyzed Dpys). Rescued by male mating. Mutagen: EMS Outcrossed: x>2 Made by: K. Kemphues Received: 02/27/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK114 Species: Caenorhabditis elegans Genotype: daf-7(e1372) par-2(it5) III. Description: Temperature sensitive-grow at 15C. Par at 25C. Strict maternal effect lethal. Mutagen: EMS Outcrossed: x3 Made by: Ken Kemphues Received: 04/21/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK1216 Species: Caenorhabditis elegans Genotype: par-3(it298[par-3::GFP]) III. Description: Superficially wild-type. Made in N2 background. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Ken Kemphues Received: 07/06/16 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK1218 Species: Caenorhabditis elegans Genotype: par-3(it300[par-3::mCherry]) III. Description: Superficially wild-type. Made in N2 background. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Ken Kemphues Received: 07/06/16 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK1228 Species: Caenorhabditis elegans Genotype: pkc-3(it309[GFP::pkc-3]) II. Description: Superficially wild-type. Note that double homozygous mutants of pkc-3(it309) with par-2(it315) exhibited partially penetrant and variable maternal effect lethality and maternal effect sterility that was stronger than it315 alone. Thus, although the GFP tag on pkc-3 shows no effect in an otherwise wild-type background, it does seem to somewhat compromise the activity of the protein it tags. Made in N2 background. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: L Coyne & K Kemphues Received: 07/06/16 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK1248 Species: Caenorhabditis elegans Genotype: par-6(it310[par-6::GFP]) I. Description: Superficially wild-type. Made in N2 background. [NOTE: There was an error in the information originally submitted to the CGC for this strain. The correct allele name is par-6(it310), not par-6(it319).] Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: A Syed & K Kemphues Received: 07/06/16 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK1254 Species: Caenorhabditis elegans Genotype: par-2(it315[mCherry::par-2]) III. Description: par-2(it315) exhibits a weak maternal effect sterility, suggesting that the tag reduces the protein activity. Note, however, that double homozygous mutants of it315 with pkc-3(it309) or par-6(it310) exhibited partially penetrant and variable maternal effect lethality and maternal effect sterility that was stronger than it315 alone. Thus, although the GFP tags on pkc-3 and par-6 show no effect in an otherwise wild-type background, they do seem to somewhat compromise the activity of the proteins they tag. Made in N2 background. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Ken Kemphues Received: 07/06/16 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK1262 Species: Caenorhabditis elegans Genotype: par-1(it324[par-1::GFP::par-1 exon11a]) V. Description: Superficially wild-type. Made in N2 background. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Diane Morton Received: 07/06/16 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK1273 Species: Caenorhabditis elegans Genotype: par-2(it328[GFP::par-2]) III. Description: Superficially wild-type. Made in N2 background. Mutagen: Crispr/Cas9 Outcrossed: x0 Made by: Ken Kemphues Received: 07/06/16 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK157 Species: Caenorhabditis elegans Genotype: mel-9(b293) II. Description: Maternal effect lethal mutation. Temperature sensitive-grow at 15C. Some growth at 20C. Does not grow at 25C. Rescued by male mating. Mutagen: EMS Outcrossed: x>3 Made by: K. Kemphues Received: 02/27/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK184 Species: Caenorhabditis elegans Genotype: par-4(it47) V. Description: Strict maternal effect lethal. Temperature sensitive-grow at 15C. Par at 20C and 25C. Mutagen: Gamma Rays Outcrossed: x>3 Made by: P. Carter/D. Morton Received: 04/21/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK204 Species: Caenorhabditis elegans Genotype: sma-4(e729) mab-5(e1239) unc-36(e251) III. Description: Small and Unc. Mutagen: Outcrossed: x Made by: Colleen Kirby Received: 04/21/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK237 Species: Caenorhabditis elegans Genotype: lon-1(e185) par-3(e2074) III; sDp3 (III;f). Description: WT sDp3-bearing animals segregate WT and lon-1 par-3 homozygotes that lay eggs that don't hatch. par-3 exhibits mildly ts penetrance in homozygotes. Strict maternal effect. Received new stock 3/01. Mutagen: EMS Outcrossed: x>3 Made by: Jim Priess Received: 04/21/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK241 Species: Caenorhabditis elegans Genotype: lon-1(e185) par-3(it62) III; sDp3 (III;f). Description: Animals with the Duplication are WT. Animals which have lost the Duplication are Lon and throw dead eggs. Mutagen: EMS Outcrossed: x10 Made by: Received: 10/31/88 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK255 Species: Caenorhabditis elegans Genotype: dpy-20(e1282) unc-22(e66) IV. Description: Dpy. Twitcher. Mutagen: Outcrossed: x Made by: Diane Morton Received: 03/27/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK26 Species: Caenorhabditis elegans Genotype: unc-4(e120) ooc-3(b310)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUnc and Unc-4 which give dead eggs. Maintain by picking WT. Strict maternal effect. Mutagen: EMS Outcrossed: x>2 Made by: Ken Kemphues Received: 03/27/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK28 Species: Caenorhabditis elegans Genotype: unc-4(e120) mel-17(b299)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Strict maternal effect. Mutagen: EMS Outcrossed: x>2 Made by: Ken Kemphues Received: 03/14/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK288 Species: Caenorhabditis elegans Genotype: sqt-3(sc8) par-1(b274) V/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Unc, RolPar (adult homozygotes lay eggs that don't hatch) and dead eggs. nT1[unc-?(n754) let-?] is dominant Unc and recessive lethal. Strict maternal effect. sc8 previously called rol-4(sc8). Mutagen: EMS Outcrossed: x>3 Made by: Ken Kemphues Received: 04/21/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK299 Species: Caenorhabditis elegans Genotype: par-5(it55) unc-22(e66) IV/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Unc, Twitchers which give only dead eggs, and dead eggs. nT1 heterozygotes are shorter and slower than par-5 unc-22 homozygous worms. par-5 region is not well balanced by nT1: check to make sure that unc-22 homozygotes lay dead eggs. Strict maternal effect lethal. Mutagen: EMS Outcrossed: x>3 Made by: Diane Morton Received: 12/17/01 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK300 Species: Caenorhabditis elegans Genotype: par-4(it57) V. Description: Temperature-sensitive maternal effect lethal (strict). Maintain at 15-16C (95% of embyros hatch). Shift to 25C for Par phenotype (no hatching embryos). Mutagen: EMS Outcrossed: x>3 Made by: Diane Morton Received: 12/17/01 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK312 Species: Caenorhabditis elegans Genotype: him-14(it44) II. Description: Maternal effect lethal mutation. High incidence of males in surviving offspring. Not rescued by male mating. Temperature sensitive. Mutagen: EMS Outcrossed: x>2 Made by: Ken Kemphues Received: 02/27/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK332 Species: Caenorhabditis elegans Genotype: mel-11(it26) unc-4(e120) sqt-1(sc13)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUnc, and UncRol (which give only dead eggs). Maintain by picking WT. mel-11 is not strict. See also WBPaper00002726. Mutagen: EMS Outcrossed: x>2 Made by: Ken Kemphues Received: 02/27/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK337 Species: Caenorhabditis elegans Genotype: unc-4(e120) mex-1(it9)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUnc and Unc-4 which give dead eggs. Maintain by picking WT. Strict maternal effect. it9 was previously called mel-21(it9). Mutagen: EMS Outcrossed: x>2 Made by: Ken Kemphues Received: 03/27/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK338 Species: Caenorhabditis elegans Genotype: mel-10(it10) unc-4(e120) sqt-1(sc13)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Strict maternal effect. Mutagen: EMS Outcrossed: x>2 Made by: Ken Kemphues Received: 03/14/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK34 Species: Caenorhabditis elegans Genotype: unc-4(e120) mel-16(b298)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Mutagen: EMS Outcrossed: x>2 Made by: Ken Kemphues Received: 03/14/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK344 Species: Caenorhabditis elegans Genotype: unc-4(e120) mel-22(it30)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUnc and Unc-4 which give dead eggs. Maintain by picking WT. Strict maternal effect. Mutagen: EMS Outcrossed: x>2 Made by: Ken Kemphues Received: 03/27/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK352 Species: Caenorhabditis elegans Genotype: unc-4(e120) top-2(it38)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Not a strict maternal effect-rescued by male mating. Mutagen: EMS Outcrossed: x>2 Made by: Ken Kemphues Received: 03/14/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK356 Species: Caenorhabditis elegans Genotype: mel-4(it12) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs (it12 is temperature sensitive). Maintain by picking WT. Strict maternal effect. Mutagen: EMS Outcrossed: x>2 Made by: Ken Kemphues Received: 01/16/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK357 Species: Caenorhabditis elegans Genotype: mel-1(it19) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Strict maternal effect. Mutagen: EMS Outcrossed: x>2 Made by: Ken Kemphues Received: 01/16/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK359 Species: Caenorhabditis elegans Genotype: tofu-6(it20) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Strict maternal effect. Mutagen: EMS Outcrossed: x>2 Made by: Ken Kemphues Received: 01/16/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK36 Species: Caenorhabditis elegans Genotype: unc-4(e120) mel-13(b306) sqt-1(sc13)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, paralysed DpyUnc and RolUnc-4 which give dead eggs. Maintain by picking WT. Strict maternal effect. Mutagen: EMS Outcrossed: x>2 Made by: Ken Kemphues Received: 03/14/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK364 Species: Caenorhabditis elegans Genotype: mel-8(it27) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: mel-8 is a maternal effect lethal. WT heterozygotes segregate WT; mel-8 unc-4 homozygotes that lay eggs that don't hatch, and mnC1 dpy-10 unc-52 homozygotes (paralyzed Dpys). Some F2 mel homozygotes hatch, but such escapers arrest at L1 or L2. Previously called mel-6(it27). Mutagen: EMS Outcrossed: x>2 Made by: Ken Kemphues Received: 01/16/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK366 Species: Caenorhabditis elegans Genotype: mel-7(it36) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Strict maternal effect. Mutagen: EMS Outcrossed: x>2 Made by: Ken Kemphues Received: 02/27/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK37 Species: Caenorhabditis elegans Genotype: mel-5(b314) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs (0.5% leaky). Maintain by picking WT. Not a strict maternal effect. Mutagen: EMS Outcrossed: x>2 Made by: Ken Kemphues Received: 01/16/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK375 Species: Caenorhabditis elegans Genotype: mel-12(it42) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Not a strict maternal effect-rescued by male mating. Mutagen: EMS Outcrossed: x>2 Made by: Ken Kemphues Received: 02/27/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK40 Species: Caenorhabditis elegans Genotype: mel-3(b281) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Strict maternal effect. Mutagen: EMS Outcrossed: x>2 Made by: Ken Kemphues Received: 01/16/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK41 Species: Caenorhabditis elegans Genotype: unc-4(e120) mel-20(b317)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Not a strict maternal effect-rescued by male mating. Mutagen: EMS Outcrossed: x>2 Made by: Ken Kemphues Received: 03/27/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK436 Species: Caenorhabditis elegans Genotype: unc-4(e120) mel-14(it24)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs (approx .2% leaky). Maintain by picking WT. Mutagen: EMS Outcrossed: x>2 Made by: Ken Kemphues Received: 03/14/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK49 Species: Caenorhabditis elegans Genotype: unc-4(e120) mel-18(b300)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUnc and Unc-4s which give only dead eggs. Maintain by picking WT. Not a strict maternal effect-rescued by male mating. Mutagen: EMS Outcrossed: x>2 Made by: Ken Kemphues Received: 03/27/89 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK571 Species: Caenorhabditis elegans Genotype: lon-1(e185) par-3(it71)/qC1 [dpy-19(e1259) glp-1(q339)] III. Description: Heterozygotes are WT and segregate WT, Dpy Steriles, and Lon which give only dead eggs. Mutagen: Outcrossed: x Made by: Received: 03/19/01 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK627 Species: Caenorhabditis elegans Genotype: itDf2 V/nT1 [unc-?(n754) let-?] (IV;V). Description: Heterozygotes are Unc and segregate Uncs and dead eggs. Mutagen: Gamma Rays Outcrossed: x Made by: Received: 04/05/93 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK696 Species: Caenorhabditis elegans Genotype: ooc-5(it145) unc-4(e120)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Heterozygotes are WT and segregate WT, DpyUncs and Sterile Uncs. it145 is a recessive mutation that results in multiple rows of small oocytes instead of a single row of normal size oocytes. it145 is a recessive maternal effect lethal mutation: hermaphrodites produce embryos that fail to hatch - the embryos exhibit a partial defect in P0 nuclear rotation and a strong defect in P1 nuclear rotation; PAR proteins are mislocalized in P1. Mutagen: EMS Outcrossed: x4 Made by: Lesilee Rose Received: 03/17/00 University of California, Davis, CA ----------------------------------------------------------------------------- Strain: KK725 Species: Caenorhabditis elegans Genotype: nop-1(it142) III. Description: Recessive maternal effect. Embryos from nop-1 mothers exhibit no pseudocleavage contractions or furrow. The majority of the embryos survive and grow into fertile adults. Mutagen: EMS Outcrossed: x4 Made by: Lesilee Rose Received: 05/18/95 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK818 Species: Caenorhabditis elegans Genotype: par-6(zu222) unc-101(m1)/hIn1 [unc-54(h1040)] I. Description: Heterozygotes are WT and segregate WT, paralyzed Unc, and Coilers which give only dead eggs (slightly leaky, but survivors are agametic). zu222 is a strict maternal effect embryonic lethal. Partitioning defect similar to that of par-3; fails to localize PAR-3 protein. Mutagen: mut-6 induced. Outcrossed: x11 Made by: Received: 11/25/96 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK822 Species: Caenorhabditis elegans Genotype: par-1(zu310) V. Description: Temperature-sensitive maternal effect lethal. Maintain at 15C. At 25C embryos show polarity defects and fail to hatch. Reference: Morton DG, Hoose WA, Kemphues KJ. Genetics. 2012 Aug 10. Mutagen: EMS Outcrossed: x8 Made by: Su Guo Received: 10/03/12 Cornell University, Ithaca, NY ----------------------------------------------------------------------------- Strain: KK866 Species: Caenorhabditis elegans Genotype: itIs153. Description: itIs153 [pie-1p::par-2::GFP + rol-6(su1006) + N2 genomic DNA]. Maintain at 25C. itIs153 is an integrated derivitive of axEx1094. Mutagen: Gamma Rays Outcrossed: x1 Made by: Wallenfang/Schetter Received: 07/31/01 Johns Hopkins University, Baltimore, MD ----------------------------------------------------------------------------- Strain: KK96 Species: Caenorhabditis elegans Genotype: unc-4(e120) mix-1(b285) sqt-1(sc13)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: mix-1(b285) is maternal effect lethal. Heterozygotes are WT and segregate WT, Unc-4 worms which are strong Egl, and paralyzed Dpy Uncs. Mutagen: Outcrossed: x Made by: Ken Kemphues Received: 10/28/10 University of California-Berkeley, CA, USA ----------------------------------------------------------------------------- Strain: KM134 Species: Caenorhabditis elegans Genotype: mef-2(gv1) I; ayIs. Description: Slightly short and fat as adults. 1376 bp deletion of part of intron 1 through intron 4. Contains an integrated hlh-8::GFP reporter marking the postembryonic M lineage. Mutagen: EMS (Barstead/Moulder) Outcrossed: x3 Made by: M. Krause Received: 09/07/00 niddk ----------------------------------------------------------------------------- Strain: KM137 Species: Caenorhabditis elegans Genotype: mef-2(gv2) I. Description: 760 bp deletion beginnin in exon 3 and ending in exon 4. No strong visible phenotype. Mutagen: EMS (Barstead/Moulder) Outcrossed: x3 Made by: M. Krause Received: 09/07/00 niddk ----------------------------------------------------------------------------- Strain: KM166 Species: Caenorhabditis elegans Genotype: cye-1(eh10)/dpy-14(e188) I. Description: Heterozygotes are WT and segregate WT, Dpys, and Sterile Pvl. Mutagen: Outcrossed: x4 Made by: Mike Krause Received: 08/19/03 niddk ----------------------------------------------------------------------------- Strain: KM48 Species: Caenorhabditis elegans Genotype: +/szT1 [lon-2(e678)] I; cdk-4(gv3)/szT1 X. Description: 745 bp deletion of cdk-4 from intron I to exon3 removing putative ATP binding domain and catalytic residues. Most homozygous animals arrest at L2 due to absence of most or all postembryonic somatic cell divisions. Some germline proliferation resulting in slightly elongated gonad. Mutagen: EMS Outcrossed: x3 Made by: Brodigan & Krause Received: 12/16/99 niddk ----------------------------------------------------------------------------- Strain: KMW1 Species: Caenorhabditis elegans Genotype: rha-1(tm329) II. Description: Strain grows normally at 16C. Egg laying is slightly delayed at 20C. Hermaphrodites and males have a maternal effect, temperature-sensitive sterile phenotype at 25.5C. Therefore, L4s shifted to 25.5C will lay offspring, but the offspring will be 90-100% sterile. Mutagen: UV/TMP Outcrossed: x8 Made by: S. Mitani Received: 06/21/05 New College of Florida ----------------------------------------------------------------------------- Strain: KN1510 Species: Caenorhabditis elegans Genotype: huIs120. Description: huIs120 [hsp-16.2p::sfrp-1 + myo-2p::Tomato]. Wild-type under normal culture conditions. Heat-shock (10 min) during embryogenesis causes embryonic lethality and HSN migration defects. Heat-shock (10 min) at hatching causes Ql and QR migration defects. Reference: Harterink M, et al. Development. 2011 Jul;138(14):2915-24. Mutagen: Gamma radiation Outcrossed: x0 Made by: Martin Harterink Received: 06/29/11 Hubrecht Institute ----------------------------------------------------------------------------- Strain: KN259 Species: Caenorhabditis elegans Genotype: huIs33. Description: huIs33 [sod-3::GFP + rol-6(su1006)]. Pick Rollers to maintain. Mutagen: Outcrossed: x Made by: Received: 04/17/06 Hubrecht Institute ----------------------------------------------------------------------------- Strain: KN369 Species: Caenorhabditis elegans Genotype: muIs32 II; egl-20(hu105) IV. Description: muIs32 [mec-7p::GFP + lin-15(+)]. Egl. Anterior migration of the QL.d, defects in QR.d and HSN migration. Mutagen: EMS Outcrossed: x3 Made by: Rik Korswagen Received: 06/23/06 Hubrecht Institute ----------------------------------------------------------------------------- Strain: KN387 Species: Caenorhabditis elegans Genotype: muIs32 II; egl-20(hu120) IV. Description: muIs32 [mec-7p::GFP + lin-15(+)]. Egl. Anterior migration of the QL.d, defects in QR.d and HSN migration. Mutagen: EMS Outcrossed: x Made by: Rik Korswagen Received: 06/23/06 Hubrecht Institute ----------------------------------------------------------------------------- Strain: KN4 Species: Caenorhabditis elegans Genotype: huIs4. Description: huIs4 [hsp-16.2p::pop-1(delta N) + rol-6(su1006)]. Rollers. Truncated pop-1 fragment encoding delta N-POP-1(45-438) driven by heat-shock promotor hsp-16.2. Reference: Korswagen et al. (2000) Nature 406: 527-32. Mutagen: Outcrossed: x0 Made by: Rik Korswagen Received: 02/12/16 Hubrecht Institute ----------------------------------------------------------------------------- Strain: KN478 Species: Caenorhabditis elegans Genotype: daf-16(mu86) I; huIs33. Description: huIs33 [sod-3::GFP + rol-6(su1006)]. Pick Rollers to maintain. Mutagen: Outcrossed: x Made by: Received: 04/17/06 Hubrecht Institute ----------------------------------------------------------------------------- Strain: KN53 Species: Caenorhabditis elegans Genotype: huIs7. Description: huIs7 [hsp-16.2p::Myc::bar-1(delta N) + mec-7p::GFP + dpy-20(+)]. Muv. Posterior migration of the QR descendants. Reference: de Groot et al. (2014) Science Signal. Ra26. Mutagen: Outcrossed: x5 Made by: Rik Korswagen Received: 02/12/16 Hubrecht Institute ----------------------------------------------------------------------------- Strain: KN555 Species: Caenorhabditis elegans Genotype: vps-35(hu68) II. Description: QL.d localize in anterior. HSN neurons fail to migrate. Strong egg-laying defect. Mutagen: Damien Coudreuse Outcrossed: x3 Made by: Tc1 Received: 06/15/06 Hubrecht Institute ----------------------------------------------------------------------------- Strain: KN562 Species: Caenorhabditis elegans Genotype: pop-1(hu9) I; muIs32 II. Description: muIs32 [mec-7p::GFP + lin-15(+)]. QL daughter cells (e.g. PVM) migrate to positions anterior of the vulva in about 25% of the animals. Mutagen: Outcrossed: x4 Made by: Rik Korswagen Received: 04/09/04 Hubrecht Institute ----------------------------------------------------------------------------- Strain: KN594 Species: Caenorhabditis elegans Genotype: cwn-1(ok546) II; egl-20(n585) IV. Description: Mutagen: Outcrossed: x Made by: Rik Korswagen Received: 09/18/06 UMBC, Baltimore, MD ----------------------------------------------------------------------------- Strain: KN611 Species: Caenorhabditis elegans Genotype: axl-1(tm1095) I. Description: tm1095 enhances Q neuroblast migration and vulva induction phenotype of pry-1. Minor defect in excretory cell development. Reference: Oosterveen et al. (2007) Dev Biol 308: 438-48. Mutagen: UV/TMP Outcrossed: x4 Made by: Tony Oosterveen Received: 02/12/16 Hubrecht Institute ----------------------------------------------------------------------------- Strain: KN637 Species: Caenorhabditis elegans Genotype: axl-1(tm1095) I; muIs32 II; bar-1(ga80) X. Description: muIs32 [mec-7p::GFP + lin-15(+)] II. Reference: Oosterveen et al. (2007) Dev Biol 308: 438-48. Mutagen: Outcrossed: x4 Made by: Tony Oosterveen Received: 02/12/16 Hubrecht Institute ----------------------------------------------------------------------------- Strain: KN644 Species: Caenorhabditis elegans Genotype: pop-1(hu9) axl-1(tm1095) I; muIs32 II. Description: muIs32 [mec-7p::GFP + lin-15(+)] II. Reference: Oosterveen et al. (2007) Dev Biol 308: 438-48. Mutagen: Outcrossed: x4 Made by: Tony Oosterveen Received: 02/12/16 Hubrecht Institute ----------------------------------------------------------------------------- Strain: KN689 Species: Caenorhabditis elegans Genotype: axl-1(tm1095) pry-1(mu38) I; muIs32 II; huEx83. Description: muIs32 [mec-7p::GFP + lin-15(+)] II. huEx83 [pry-1p::axl-1::GFP + myo-2p::GFP]. Pick animals with GFP+ pharynx to maintain. Reference: Oosterveen et al. (2007) Dev Biol 308: 438-48. Mutagen: Outcrossed: x Made by: Tony Oosterveen Received: 02/12/16 Hubrecht Institute ----------------------------------------------------------------------------- Strain: KN720 Species: Caenorhabditis elegans Genotype: dpy-20(e1282) IV; huIs60. Description: huIs60 [egl-20p::egl-20::protA + dpy-20(+)]. Mutagen: Gamma Rays Outcrossed: x Made by: Received: 06/15/06 Hubrecht Institute ----------------------------------------------------------------------------- Strain: KN800 Species: Caenorhabditis elegans Genotype: lon-8(hu187) V. Description: Long body size. Mutagen: UV/TMP Outcrossed: x3 Made by: Gwen Soete Received: 05/29/07 Hubrecht Institute ----------------------------------------------------------------------------- Strain: KN846 Species: Caenorhabditis elegans Genotype: lon-8(hu188) V. Description: Long body size. Mutagen: UV/TMP Outcrossed: x3 Made by: Gwen Soete Received: 05/29/07 Hubrecht Institute ----------------------------------------------------------------------------- Strain: KP1008 Species: Caenorhabditis elegans Genotype: egl-36(n728n398) X. Description: Reference: Elkes DA, et al. Neuron. 1997 Jul;19(1):165-74. Mutagen: Outcrossed: x Made by: Kaplan Lab Received: 06/10/13 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: KP1097 Species: Caenorhabditis elegans Genotype: dgk-1(nu62) X. Description: Hyperactive locomotion and egg-laying. Hypersensitive to aldicarb. Suppresses goa-1(gf). Allelic to sag-1. Mutagen: EMS Outcrossed: x2 Made by: S. Nurrish Received: 11/01/99 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: KP1148 Species: Caenorhabditis elegans Genotype: nuIs25. Description: nuIs25 [glr-1p::glr-1::GFP + lin-15(+)]. Reference: Rango C, et al. Cell. 1998 Sep 18;94(6):751-9. Mutagen: Outcrossed: x Made by: Chris Rongo Received: 07/05/14 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: KP1182 Species: Caenorhabditis elegans Genotype: acy-1(nu329) III. Description: No obvious phenotype on its own. Suppresses cell swelling and death caused by expression of a GTPase defective Gxs subunit expressed from the glr-1 promoter, semi-dominant. Strongest allele. Mutagen: Outcrossed: x Made by: Allison Berger Received: 09/11/98 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: KP1287 Species: Caenorhabditis elegans Genotype: nuIs26 IV. Description: nuIs26 [cat-1::GFP] IV. nuIs26 contains a full length CAT-1::GFP fusion protein (with GFP fused at the predicted carboxy terminus of CAT-1). This transgene rescues the hyperactive locomotion defect of cat-1(e1111). This strain is slightly sluggish for locomotion and has a high degree of sterility (80%), both of which appear to be associated with the transgene. GFP expression in this strain is similar to the CAT expression pattern described by Duerr et al (1999) J Neurosci 19: 72-84. Mutagen: Outcrossed: x2 Made by: S. Nurrish Received: 09/10/01 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: KP2018 Species: Caenorhabditis elegans Genotype: egl-21(n476) IV. Description: Mutation confirmed by Nonet lab. Mutagen: EMS Outcrossed: x5 Made by: Received: 06/12/06 Washington University, St. Louis, MO ----------------------------------------------------------------------------- Strain: KP2048 Species: Caenorhabditis elegans Genotype: ric-7(nu447) V. Description: Reference: Hao Y, et al. PLoS Genet. 2012 Jan;8(1):e1002464. Mutagen: Outcrossed: x4 Made by: Kaplan Lab Received: 06/10/13 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: KP2342 Species: Caenorhabditis elegans Genotype: pkc-1(nu448) V. Description: Mutagen: EMS Outcrossed: x Made by: Derek Sieburth Received: 04/06/07 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: KP2932 Species: Caenorhabditis elegans Genotype: lin-23(nu412)/mnC1 [dpy-10(e128) unc-52(e444)] II. Description: Mutagen: EMS Outcrossed: x1 Made by: Lars Derier Received: 04/06/07 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: KP3085 Species: C. elegans Genotype: nuIs122 IV. Description: nuIs122 [acr-2p::pHluorin::snb-1 + myo-2p::dsRed2] IV. Integrated transgene expressing synaptopHluorin under a cholinergic promoter for imaging motor neuron synapses. Mutagen: Outcrossed: x8 Made by: Jeremy Dittman Received: 08/19/19 Cornell University Medical College, New York, NY ----------------------------------------------------------------------------- Strain: KP3814 Species: C. elegans Genotype: nuIs152 II. Description: nuIs152 [unc-129p::GFP::snb-1 + ttx-3p::mRFP] II. Mutagen: none Outcrossed: x2 Made by: Ch'ng Q and Sieburth D Received: 06/06/17 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: KP3947 Species: Caenorhabditis elegans Genotype: nuIs183. Description: nuIs183 [unc-129p::nlp-21::Venus + myo-2p::GFP]. [NOTE: nuIs183 was previously described as carrying myo-2p::NLS::GFP.] Reference: Sieburth D, et al. Nat Neurosci. 2007 Jan;10(1):49-57. Mutagen: Outcrossed: x10 Made by: Joshua Kaplan Received: 03/20/13 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: KP3948 Species: Caenorhabditis elegans Genotype: eri-1(mg366) IV; lin-15B(n744) X. Description: Enhanced RNAi. Temperature sensitive sterile at 25C. Grow at 15 to 20C. Mutagen: EMS Outcrossed: x4 Made by: S Kennedy/D Wang Received: 08/10/05 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: KP3957 Species: Caenorhabditis elegans Genotype: nuIs190 X. Description: nuIs190 [unc-129p::ins-22::Venus + myo-2p::NLS::GFP] X. Pick GFP+ to maintain. Not all animals express GFP. Array might be prone to silencing or not homozygous. [NOTE: nuIs190 was previously described as carrying myo-2p::GFP without an NLS.] Reference: Ch'ng Q, Sieburth D, Kaplan JM. PLoS Genet. 2008 Nov;4(11):e1000283. Mutagen: Outcrossed: x4 Made by: D Sieburth & Q Ch'ng Received: 08/06/14 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: KP3972 Species: C. elegans Genotype: nuIs184 X. Description: nuIs184 [unc-129p::apt-4::GFP + myo-2p::GFP] X. Mutagen: none Outcrossed: x3 Made by: : Ch'ng Q and Sieburth D Received: 06/06/17 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: KP3991 Species: Caenorhabditis elegans Genotype: nuIs145 V. Description: nuIs145 [glr-1p::vps-4(dominant negative)] V. Reference: Chun DK, et al. Mol Biol Cell. 2008 Jul;19(7):2682-95. Mutagen: Outcrossed: x Made by: Kaplan Lab Received: 06/10/13 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: KP4 Species: Caenorhabditis elegans Genotype: glr-1(n2461) III. Description: pka not-3. Loss of function allele. Defective in response to nose touch but not to osmotic repellents. Mutagen: EMS Outcrossed: x4 Made by: J. Kaplan Received: 12/19/95 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: KP4211 Species: C. elegans Genotype: nuIs214 III. Description: nuIs214 [unc-129p::itsn-1::GFP + myo-2p::GFP] III. Mutagen: none Outcrossed: x3 Made by: Ch'ng Q and Sieburth D Received: 06/06/17 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: KP5445 Species: C. elegans Genotype: nuIs165. Description: nuIs165 [unc-129p::unc-10::GFP + myo-2p::GFP] II. Mutagen: N/A Outcrossed: x3 Made by: Ch'ng Q and Sieburth D Received: 06/06/17 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: KP5832 Species: Caenorhabditis elegans Genotype: unc-108(nu415) I. Description: Reference: Chun DK, et al. Mol Biol Cell. 2012; 19, 2682-95. Mutagen: Outcrossed: x Made by: JM Kaplan Received: 06/04/12 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: KP6200 Species: Caenorhabditis elegans Genotype: ckr-2(tm3082) III. Description: Reference: Hu Z, et al. Neuron. 2011 Jul 14;71(1):92-102. Mutagen: Outcrossed: x Made by: Josh Kaplan Received: 11/28/12 Massachusetts General Hospital, Boston, MA ----------------------------------------------------------------------------- Strain: KP696 Species: Caenorhabditis elegans Genotype: eos-2(nu268) III. Description: Weak Osm as homozygote. Non-allelic non-complementor of osm-10(n1602). Mutagen: EMS Outcrossed: x0 Made by: J. Kass Received: 01/18/00 Brown University ----------------------------------------------------------------------------- Strain: KP715 Species: Caenorhabditis elegans Genotype: eos-1(nu288) IV. Description: Weak Osm as homozygote. Non-allelic non-complementor of osm-10(n1602). Mutagen: EMS Outcrossed: x0 Made by: J. Kass Received: 01/18/00 Brown University ----------------------------------------------------------------------------- Strain: KP987 Species: Caenorhabditis elegans Genotype: lin-15B&lin-15A(n765) nuIs1 X. Description: nuIs1 [glr-1p::GFP + glr-1(+) + lin-15(+)] X. GFP expression in 17 classes of neurons after 3-fold (see WBPaper00002309). This strain is WT at glr-1. Mutagen: Outcrossed: x5 Made by: Anne Hart Received: 03/19/01 Brown University ----------------------------------------------------------------------------- Strain: KQ1323 Species: Caenorhabditis elegans Genotype: akt-2(tm812) sgk-1(ft15) X. Description: ft15 allele is a gain-of-function mutation in sgk-1. sgk-1(ft15) mutants have normal body size and normalize the small body size of rict-1 loss-of-function mutants. Reference: Jones KT, et al. PLos Biol. 2009 Mar 3;7(3):e60. doi: 10.1371/journal.pbio.1000060. PMID: 19260765. Mutagen: EMS Outcrossed: x2 Made by: Kevin Jones Received: 07/01/09 University of California, San Francisco, CA ----------------------------------------------------------------------------- Strain: KQ1366 Species: Caenorhabditis elegans Genotype: rict-1(ft7) II. Description: Small, slightly developmentally delayed. Increased Nile Red staining. Slightly more severe than mg360. Mutagen: EMS Outcrossed: x3 Made by: K Jones/E Greer Received: 04/27/09 University of California, San Francisco, CA ----------------------------------------------------------------------------- Strain: KQ1564 Species: Caenorhabditis elegans Genotype: sgk-1(ft15) X. Description: ft15 allele is a gain-of-function mutation in sgk-1. sgk-1(ft15) mutants have normal body size and normalize the small body size of rict-1 loss-of-function mutants. Reference: Jones KT, et al. PLos Biol. 2009 Mar 3;7(3):e60. doi: 10.1371/journal.pbio.1000060. PMID: 19260765. Mutagen: EMS Outcrossed: x3 Made by: Kevin Jones Received: 07/01/09 University of California, San Francisco, CA ----------------------------------------------------------------------------- Strain: KQ2841 Species: Caenorhabditis elegans Genotype: aat-1(ft1003) IV. Description: sf1003 is a CRISPR-engineered deletion in aat-1. aat-1(sf1003) mutants show elevated pumping rates and enhanced learning capacity compared to well-fed wild type animals (similar to nkat-1 mutants). Reference: Lin L, et al. Genes Dev. 2020 Aug 1;34(15-16):1033-1038. doi: 10.1101/gad.339119.120. PMID: 32675325. Mutagen: Crispr/Cas9 Outcrossed: x4 Made by: Lin Lin Received: 07/11/22 University of California, San Francisco, CA ----------------------------------------------------------------------------- Strain: KQ6 Species: Caenorhabditis elegans Genotype: rict-1(mg360) II. Description: Small, slightly developmentally delayed. Increased Nile Red staining. Mutagen: EMS Outcrossed: x3 Made by: Kevin Jones Received: 04/27/09 University of California, San Francisco, CA ----------------------------------------------------------------------------- Strain: KR1037 Species: Caenorhabditis elegans Genotype: unc-13(e51)/hT1 I; dpy-11(e224)/hT1 [unc-42(e270)] V. Description: Pick wild-type to maintain. Segregates wild-type, Dpy Unc, arrested hT1 homozygotes, and dead eggs. Reference: McKim KS, et al. Genetics. 1988 Dec;120(4):987-1001. Mutagen: Outcrossed: x0 Made by: Kim McKim Received: 06/10/13 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: KR1054 Species: Caenorhabditis elegans Genotype: dpy-5(e61) unc-13(e450)/hT1 I; +/hT1 [unc-42(e270)] V. Description: Pick wild-type to maintain. Segregates wild-type, Dpy Unc, arrested hT1 homozygotes, and dead eggs. Reference: McKim KS, et al. Genetics. 1988 Dec;120(4):987-1001. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to Ann Rose. Mutagen: Gamma Rays Outcrossed: x2 Made by: Kim McKim Received: 06/10/13 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: KR1107 Species: Caenorhabditis elegans Genotype: unc-11(e47) dpy-5(e61) I; hDp10 (I;f). Description: Dpy. Segregatess DpyUncs. Slow. DpyUnc will take over. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to Ann Rose. Mutagen: gamma on KR16 Outcrossed: x Made by: Kim McKim Received: 09/16/92 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: KR1108 Species: Caenorhabditis elegans Genotype: unc-11(e47) dpy-5(e61) I; hDp8 (I;f). Description: Dpy-5 phenotype. Segregates Dpy-5 and Unc-11 Dpy-5 progeny. Duplication-bearing animals are slow-growing, and UncDpys will overgrow the population. Pick several Dpy-5 animals and check for correct segregation of progeny to maintain. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to Ann Rose. Mutagen: 3000 R gamma Outcrossed: x0 Made by: McKim K Received: 05/16/94 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: KR1110 Species: Caenorhabditis elegans Genotype: unc-11(e47) dpy-5(e61) I; hDp11 (I;f). Description: Dpy-5 phenotype. Segregates Dpy-5, Unc-11 Dpy-5. Duplication-bearing animals are slow-growing, and UncDpys will take over population. Pick several Dpy-5 animals and check for correct segregation of progeny to maintain. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to Ann Rose. Mutagen: 3000 R gamma Outcrossed: x0 Made by: McKim K Received: 05/16/95 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: KR1112 Species: Caenorhabditis elegans Genotype: unc-11(e47) dpy-5(e61) I; hDp9 (I;f). Description: Dpy-5 phenotype. Segregates Dpy-5, Unc-11 Dpy-5. Duplication-bearing animals are slow-growing, and UncDpys will take over population. Pick several Dpy-5 animals and check for correct segregation of progeny to maintain. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to Ann Rose. Mutagen: 1500 R gamma Outcrossed: x0 Made by: McKim K Received: 04/25/95 University of British Columbia, Vancouver ----------------------------------------------------------------------------- Strain: KR1115 Species: Caenorhabditis elegans Genotype: dpy-5(e61) unc-13(e450) hDf9/szT1 [lon-2(e678)] I; +/szT1 X. Description: Wild-type phenotype, Him. Segregates wild-type, Lon-2 males and two classes of arrested embryos (dpy-5 unc-13 hDf9 homozygotes and szT1 aneuploids). Lon males are fertile, carrying hDf9 and both szT1 half translocations