Search Strains

Heterozygotes are WT and segregate WT and dead eggs. (Chromosome carrying unc-42 ama-2 is homozygous lethal.) Strain is resistant to _-amanitin.

DpyUnc.

DpyUnc.

Dominant loopy Unc with exaggerated sine wave.

More resistant to alpha-amanitin than ama-1(m118). Will grow in the presence of 0.5% triton-X 100 with 100 ug/ml amanitin whereas ama-1(m118) will not. DR1099 displays temperature-sensitive defects consistent with defective RNA Pol II function. Sterile at 25C (maternal effect sterile). Fertile at 20C, but produces few viable progeny (~80% eggs that do not hatch). Periodically check for Emb to prevent spontaneously suppressed animals from taking over a population. Reference: Rogalski TM, et al. Genetics. 1990 Dec;126(4):889-98.

Heterozygotes are WT and segregate WT, Lethal Twitchers, and dead eggs. Lethal mid-larval (leaky). Maintain by picking WT. Heterozygotes twitch in 1% nicotine.

Unc-44 masks the Unc-24 phenotype. Paralyzed coiler, Dpy, tends to curl.

Heterozygotes are WT and segregate WT, dead eggs and Twitchers that arrest as larvae. Maintain by picking WT. Hets twitch in 1% nicotine.

Blistered. Mechanosensory abnormal.

Dauer defective. Dpy.

Dauer defective. Dpy.

Dauer defective. Unc-coiler.

Dauer defective. Unc-coiler.

WT phenotype. Segregates WT and DpyUncs. mDp4 carries dpy-13(+) and unc-24(+). Duplication may recombine with normal homologues. Presence of unc-17(e245) on mDp4 confirmed: got Unc-17 segregants after heat shock of this stock to generate males. Pick WT and check for segregation of progeny to maintain.

Temperature sensitive dauer constitutive. Dauer recovery poor at 15C.

Dominant Twitcher Unc. Semi-dominant Dpy.

Dpy. Unc.

Temperature sensitive dauer constitutive . Dpy. Maternal effect.

Kinky. Coiler.

Temperature sensitive dauer constitutive. Dpy. Leaky double at 25C.

Temperature sensitive dauer constitutive. Unc.

Weak Unc (kinker) as adult. Increased life span. Maintain at 15C.

Approximately 30% dauer at 27C. Probably thermotolerant because it comes from a thermotolerant selection, but it was not retested. Increaesed longevity. Maintain at 15C.

daf-d. Maintain at 15C.

daf-d on starvation. Sterile at 25C. Maintain at 15C. Mild Unc.

Dpy. Segregates males.

Temperature sensitive dauer constitutive. Unc.

Temperature sensitive dauer constitutive. Dominant Twitcher

Dpy. Unc-slow moving.

Dpy. Unc-slow moving.

Unc-coiler. Segregates males. M-MATING-NO SUCCESS.

Dpy. Heterozygotes Roll. Segregates males. M-MATING-NO SUCCESS.

Semi-dominant Unc-shaker. Segregates males.

Dpy. Segregates males. Semi-dominant.

Severe Unc. Segregates males. M-MATING-NO SUCCESS. Semi-dominant. Heterozygotes are slow moving.

Dauer defective. Segregates males. Chemotaxis defective (Na+).

Temperature sensitive dauer constitutive. 100% dauers at 25C. Dauer recovery poor at 15C. Maternal effect. Egg retainer.

Heterozygotes are semi-Dpy and segregate semi-Dpy, Dpy and dauers. Dauers are lethal and SDS-sensitive. NOTE: WT recombinants that have lost dpy-13 can quickly overtake a population.

Heterozygotes are WT and segregate WT, Unc, and lethal dauers. Dauers are SDS-sensitive.

DpyUnc.

Temperature sensitive dauer constitutive. Unc.

Unc-Twitcher. Dominant. Genotype questionable.

Heterozygotes are WT and segregate WT, Vul, and lethals. Lethal early larval. Heterozygotes twitch in 1% nicotine. Well balanced.

Strong Twitcher. Recessive.

Heterozygotes are WT and segregate WT, Vul and lethals. Lethal early larval. Heterozygotes twitch in 1% nicotine.

Hypercontracted and amanitin resistant.

Heterozygotes are semi-Unc and segregate semi-Unc and Vul and dead eggs. (Does not segregate Unc homozygotes because linked to the unc is a lethal in the amanitin gene.)

Slow movement.