| CB1393 |
C. elegans |
daf-8(e1393) I. Show Description
Dauer constitutive. Temperature sensitive. Crowds. Chemotaxis normal. Grow at 15C.
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| PJ1195 |
C. elegans |
daf-8(e1393) I; ccIs55 V. Show Description
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Maintain at 15C. Temperature-sensitive; constitutive dauer formation at 25C.
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| BW1046 |
C. elegans |
unc-37(e262) daf-8(e1393) I. Show Description
Unc. Constitutive dauer formation (Daf-c) at 25C.
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| BW635 |
C. elegans |
lin-10(e1438) daf-8(e1393) I. Show Description
Maintain at 15C. Vulvaless. Temperature-sensitive constitutive dauer (Daf-c) at 25C.
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| BW708 |
C. elegans |
lin-28(n719) daf-8(e1393) I. Show Description
Egl. Constitutive dauer (Daf-c) at 25C.
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| DR137 |
C. elegans |
dpy-5(e61) daf-8(e1393) I. Show Description
Temperature sensitive dauer constitutive. Dpy.
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| DR168 |
C. elegans |
unc-13(e51) daf-8(e1393) I. Show Description
Temperature sensitive dauer constitutive. Unc. Semi-paralyzed. Closely linked.
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| DR184 |
C. elegans |
daf-8(e1393) I; daf-10(e1387) IV. Show Description
Temperature sensitive dauer constitutive. Dauer recovery poor at 15C.
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| DR443 |
C. elegans |
dpy-14(e188) daf-8(e1393) I. Show Description
Temperature sensitive dauer constitutive. Dpy.
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| DR848 |
C. elegans |
daf-8(e1393) unc-29(e1072) I. Show Description
Unc-weak kinker, doesn't back well. Temperature sensitive dauer constitutive. Maintain at 15C. Makes high percentage of dauers at 25C. tsp for dauer formation is L1 molt. Type C Egl. Active. Resistant to 1 mM levamisole. Sensitive to hypoosmotic shock.
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| DR927 |
C. elegans |
daf-8(e1393) fer-1(hc1) I. Show Description
Temperature sensitive dauer constitutive. Temperature sensitive fertilization defective. WT at 15C, segregates some dauers at 20C, sterile at 25C. Tightly linked.
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| TY3581 |
C. elegans |
daf-8(e1393) I; scd-2(y386) V. Show Description
Egl. Not temperature sensitive -- can be grown at any temperature.
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| HR767 |
C. elegans |
daf-8(e1393) mel-26(ct61sb4) I. Show Description
ct61 is a dominant, temperature sensitive, maternal effect lethal. sb4 is a revertant of the dominant phenotype. sb4 is a recessive, temperature sensitive, maternal effect lethal with approx. 10% hatching at 15C and 0% at 25C.
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| PJ1276 |
C. elegans |
daf-8(e1393) I; daf-14(m77) IV; ccIs55 V. Show Description
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Temperature-sensitive. Some dauer formation at 16C.
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| DR189 |
C. elegans |
dpy-5(e61) daf-8(e1393) unc-29(e1072) I. Show Description
Temperature sensitive dauer constitutive. Dpy. Unc.
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| DR943 |
C. elegans |
daf-8(e1393) fer-1(hc1) unc-29(e1072) I. Show Description
Unc. Temperature sensitive dauer constitutive. Temperature sensitive fertilization defective (late). UncDaf at 25C, Unc at 15C.
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| JT10189 |
C. elegans |
daf-14(m77) IV; scd-2(sa935) V. Show Description
Daf-c strongly but not completely suppressed; some dauers visible at 25C. sa935 alone is moderate Daf-d (dauer defective) in plate starvation assays, and resistant to exogenous dauer pheromone. sa935 confers strong suppression of daf-8 and daf-14, weak suppression of daf-1, -4, -7. scd-2(sa935) single mutant looks WT. snb-1(md247) hypomorph is an excellent balancer for scd-2; snb-1 is immediately neighboring. Maintain at 15 degrees.
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| OP790 |
C. elegans |
unc-119(tm4063) III; wgIs790. Show Description
wgIs790 [daf-8::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov M, et al. Nat Methods (2006) 10:839-44. Zhong, M, et al. PLoS Genet (2010) 6(2):e1000848. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org).
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| SYS605 |
C. elegans |
daf-8(dev177([mNeonGreen::daf-8]) I; ujIs113 II. Show Description
ujIs113 [pie-1p::mCherry::H2B::pie-1 3'UTR + nhr-2p::mCherry::his-24::let-858 3Â’UTR + unc-119(+)] II. mNeonGreen knockin at N-terminus of daf-8 locus. Cellular protein expression pattern during embryogenesis (until bean stage) is available at http://dulab.genetics.ac.cn/TF-atlas. Reference: Ma X, Zhao Z, Xiao L, et al. Nat Methods. 2021;18(8):893-902. doi:10.1038/s41592-021-01216-1.
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