Search Strains

Catecholamine abnormal. Recessive. M-MATING++ 1-10%WT. Previously called ctl-2.

Migration defective: pre VC. Variable expressivity. Reverse kinker as adult. L1 moves well. Temperature sensitive. Can be maintained at 20C.

Piggy phenotype (severe Dpy). Growth slow. Poor viability.

Unc-kinky backing. Slow moving.

Slow moving Unc. Paramyosin abnormal.

Slow moving Unc. Body Muscle abnormal. M-MATING-NO SUCCESS.

Unc. Slow moving.

Faint or invisible alae in L1. Segregates males.

Slow moving Unc. Recessive. M-MATING+POOR <1%WT.

tra-2 suppressed. Viable hermaphrodite. See also CGC 397. Grow between 22.5C and 24C.

tra-3 suppressed. Viable hermaphrodite. See also CGC 397.

Heterozygotes are wild-type, and segregate wild-type heterozygotes, fertile wild-type males (tra-1 homozygotes), and Dpy. Can recombine: pick individual WT hermaphrodites and check for correct segregation of progeny to maintain.

Heterozygotes are slow moving. Segregates paralysed small. eDf1 eDp21 homozygotes die in larval development.

Slow moving Unc. Body muscle abnormal. Temperature sensitive. Recessive. M-MATING+POOR <1%WT.

Selfed hermaphrodites produce 5% males and 2% XXX hermaphrodites (percent of viable progeny), and 30% unhatched eggs (percent of total progeny).

Temperature sensitive lethal: inviable at 25C, grows at 20C and 15C. Somewhat sickly. Small brood sizes. At 25C homozygotes have a starved translucent appearance, facilitating Nomarski visualization of neuron processes.

Homozygous viable and looks almost normal by dissecting scope, but under Nomarski microscopy the gut has a very abnormal spongy appearance, which is especially noticeable in L4 larvae.

Variably Dpy, slow growing. Inviable on MYOB medium.

Poorly balanced. Hets are WT and segregate WT, Dpys and ect-2 homozygotes. ect-2 homozygotes are sterile Uncs which reach adulthood, sometimes giving polynucleate oocytes. ect-2 pka let-21.

Dpy. Severe. Semidominant. Inviable at 15C.

Heterozygotes are WT and segregate WT, Dpy, and Long Thin Steriles (adults lay no or few eggs). lin-23 animals have excess cell divisions in larval blast cell lineages.

Heterozygotes are Unc and segregate Unc, dead eggs, and Lon males. e1913 is a dominant Unc and recessive lethal. Maintain by picking Unc. e1913 previously called unc-110.

Heterozygotes are weakly Dpy and segregate more weak Dpys, dpy-13 homozygotes and ncl-2 homozygotes. ncl-2 homozygotes are viable, sterile animals with abnormal nucleoli (abnormal refractile appearance by Nomarski). Imperfectly balanced: May Break Down!!

Variable failures in cytokinesis. Mostly viable and fertile.

Severe roundheaded Dpy. Almost inviable at 15C.

Heterozygotes are Unc (shaker; don't move well) and segregate more Shaker Unc, WT and DpyUnc (short and fairly paralyzed). The WT give only males. e1957 previously called sup-21.

Suppressed Dpy. e1957 previously called sup-21.

Obligate XX female, XO male strain. Maintain by mating. Viable 15-25C. e2046 is a dominant mutation transforming XX into fertile female; minimal effect on XO.

Weakest known fem-3 mutation. Viable non-temperature-sensitive self-fertile XX hermaphrodites. e1999 XO males are slightly feminized, sometimes male-fertile. Reference: Hodgkin (1986) PMID: 3770465.

Temperature sensitive. Sterile at 25C. Maintain at 15C. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. [2/98: Craig Mello noticed a different embryonic phenotype in this strain as compared to the e2141 stock that Jim Priess obtained from England-the ABp fate appears WT.] [NOTE (11/16/10 - J. Hubbard): This strain is NOT synonymous with glp-1(e2144) as previously reported in Kodoyianni V, Maine EM, Kimble J. (1992) [Molecular basis of loss-of-function mutations in the glp-1 gene of Caenorhabditis elegans. Mol Biol Cell. 3,1199-213. PMID: 1457827]. As reported in Worm Breeders Gazette December 2010; 18(3), e2144 carries the mutation c2785t in exon 8, leading to the amino acid change L929F, whereas e2141 carries the mutations c2920t and a3610g in exon 8, leading to the amino acid changes R974C and T1204A.]

Phenotypically wildtype XX hermaphrodite at 20C; poor viability and fertility at 25C. Rare XO animals are hermaphrodite due to suppression of amber mutation e2115 and consequent transformation of XO due to tra-2(e2046) gain-of-function. Reference: Hodgkin & Albertson (1995) PMID: 8647390.

Viable Dpy at 15C. Extreme Dpy or L1 lethal at 25C.

Long, especially obvious in adults. Males mate well.

Spontaneous mutation in TR679. Adults slightly Unc, slow and loopy. Larvae more affected, slow moving.

ZZ21 lev-1(x21) tra-3(e2333) isolated by Jim Lewis as EMS-induced levamisole resistant derivative of N2. Found to carry a cryptic tra-3 allele by Jonathan Hodgkin. XX animals are viable hermaphrodites which produce 500 rather than 330 self-progeny; occasionally Egl; no other signs of masculinization.

Wild type. previously called unc-110.

Dpy hermaphrodites. Protruding vulva in adult; viable at all temperatures.

Dpy hermaphrodites. Protruding vulva in adult; viable at all temperatures.

y1 is a temperature sensitive mutation affecting only XX animals. him-8(e1489) causes X non-disjunction, resulting in many males. Grow at 15C. XX and XXX animals not viable above 15C. After shifting to 25C, surviving population is almost all XO males.

Wild type. Low Tc1 copy number; pattern X. [Obtained from Rothamsted by S. Brenner as 'Panagrellus redivivus'. Cross fertile with C. elegans N2.] Caenorhabditis elegans wild isolate. CB subclone of N3 (Tc1 pattern X). For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Maternally rescued. Homozygous e2519 worms coming from homozygous mothers develop slowly. Adult rhythmic behaviors are also slowed.

Heterozygotes are WT and segregate WT, Lethals (L2/L3 arrest at 20C, some survive to sterile adults at 15C), and DpyRol. Not balanced well: get recombinants. Must maintain by picking hets at each generation and check for proper segregants in F1.

dpy-26(n199) is XO viable, XX lethal. eDp26 is a duplication of the left end of X, attached to X in an inverted orientation. Strain gives non-Dpy Egl hermaphrodites, intersexes, males and dead eggs.

eDp26 is a duplication of the left end of X, attached to X in an inverted orientation. CB4950 is homozygous for eDp26, which is XX viable and XO lethal. XX hermaphrodites are slightly small and slightly Egl.

At 20C or 25C heterozygotes are semi-Dpy and segregate semi-Dpys (heterozygotes), Dpys (dpy-25/dpy-25) and animals which arrest as Dpyish young larvae (let-552/let-552). Maintain by picking semi-Dpy. At 15C it is difficult to distinguish between let-552 homozygotes and dpy-25 homozygotes (e817 is inviable at 15C).

Slow growth and movement. Egl. Somewhat Uncoordinated. Maternal effect: m+z- homozygotes have no obvious phenotype.

Obligate XO male/female strain, propagate by crossing. Low fertility. Fertile XO females and XO males; inviable XX embryos and larvae. Reference: Strain 7 Hodgkin (2002) PMID: 12399387.

eEx36 [F16E1 + rol-6(su1006)]. eEx36 carries multiple copies of fox-1, which confers a Xol phenotype and pRF4 which confers a Rol phenotype. dpy-26(n199) is XO viable and XX lethal. Strain consists mostly of Rol hermaphrodites and non-Rol males, all XO.

Slow growth. Maternal rescue: m+z- homozygotes have no obvious phenotype.

Male-female strain. Reference WBG 9(3) 121. Isolated from sugar cane in Trinidad by D.J. Hunt (Commonwealth Institute of Parasitology). [7/7/93: Scott Baird and David Fitch question whether this strain is really C. remanei.] [7/95: Not cross-fertile with the authentic C. remanei isolated by Walter Sudhaus.] Previously called C. remanei by the CGC. Walter Sudhaus has tentatively described this strain as Caenorhabditis sp.; this description is not offical and is contigent upon its being published. See WBPaper00002633 and WBPaper00003993. sp. 4 in Kiontke and Sudhaus Wormbook Ecology chapter.