Search Strains

Small Dpy.

Unc. Suppressed by sup-5 and sup-7. Null allele? Milder than most null.

Paralyzed Unc. Severe phenotype. Recessive. Null allele.

Dpy. Heterozygotes Roll.

Temperature sensitive dauer constitutive. 100% dauers at 25C. Dauer recovery poor at 15C. Small. M-Mating--no success.

Body muscle abnormal. Movement almost WT. Slightly small. M-MATING+++ 10-30%WT.

Suppressor. Null allele. Sterile at 15C. Grow between 22.5 and 24C.

Shrinker-contracts both dorsally and ventrally when prodded. Slow, poor backing. Slightly small.

Long. Abnormal bursae. Epistatic to Small. Recessive. M-MATING-NO SUCCESS.

Semi-paralyzed Unc. Null allele. Recessive. M-MATING-NO SUCCESS.

Small. Unc. Linked closely.

Growth slow. Unc and Small. Revertible. M-MATING-NO SUCCESS.

Severe dumpy (piggy), grows poorly. Behaves as a null allele. Sequenced: early nonsense mutation R4opal.

Heterozygotes are slow moving. Segregates paralysed small. eDf1 eDp21 homozygotes die in larval development.

Unc. Small. Egg Retainer. Serotonin Def. Recessive. M-MATING-NO SUCCESS.

Heterozygotes are WT. Segregates Dpy. Segregates sterile Unc--Unc cannot back. Balanced well.

Pick Unc hermaphrodite to maintain (these will be tra-1/+; unc-42 him-5 dpy-21 XO animals). Segregates Unc hermaphrodites (tra-1/+ III), Unc males (+/+ III), and DpyUnc hermaphrodites (+/+ III). dpy-21 not expressed in XO. dpy-21(e428) V; XX animals are weak Dpy. dpy-21(e428) V; XO animals are non-Dpy.

Balanced well. Animals with the duplication are WT. Animals which have lost the duplication have a hermaphrodite gonad and intestine (they are self-fertile); the rest of the body is male.

Balanced well. Animals with the duplication are WT. Animals which have lost the duplication have a hermaphrodite gonad and intestine (they are self-fertile); the rest of the body is male.

Balances well. Transformer weak. Heterozygotes are WT and segregate WT, DpyUnc and males. Maintain by picking WT.

Small. Recessive. Abnormal bursae. Males abnormal. M-MATING-NO SUCCESS. See also WBPaper00003721.

Slightly Unc. Suppressed by sup-5 and sup-7. Null allele?

Caenorhabditis elegans wild isolate. Apparently identical to N2. Isolated by Carl Johnson in June 1973 in a garden in Altadena, CA. Sample obtained from laboratory of R.E. Russell in October 1980. Sent to CGC in Jan. 1997. Reference WBG 10(2) 140-141. For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Delayed hatching from egg shell. QL and descendant cells migrate forward instead of backward (incomplete penetrance).

Abnormal cell death.

Temperature sensitive lethal: inviable at 25C, grows at 20C and 15C. Somewhat sickly. Small brood sizes. At 25C homozygotes have a starved translucent appearance, facilitating Nomarski visualization of neuron processes.

Abnormal cell death.

Hermaphrodites are Dpy. Males are non-Dpy and have abnormal bursae in adult, with swollen rays. Males will mate, but with very low efficiency. [3/98: King Chow isolated a line from the CGC stock that was throwing 100% Mabs. Sent the strain back to the CGC to replace the old stock.]

Abnormal large nucleoli in all cells.

e1873 allele is the only anc-1 allele in which the molecular lesion has been identified (Starr and Han, 2002) and is considered by the authors to be the reference allele. e1873 does not revert, unlike most other alleles of anc-1. Reference: Hedgecock and Thomson. Cell. 1982 Aug;30(1):321-30. Starr and Han. Science. 2002 Oct 11;298(5592):406-9.

Heterozygotes are WT and segregate WT, Dpy, and Long Thin Steriles (adults lay no or few eggs). lin-23 animals have excess cell divisions in larval blast cell lineages.

Paralyzed Unc. Small. Recessive. M-MATING-NO SUCCESS.

Slightly Unc. Suppressed by sup-5 and sup-7. Null allele?

Heterozygotes are Unc (shaker; don't move well) and segregate more Shaker Unc, WT and DpyUnc (short and fairly paralyzed). The WT give only males. e1957 previously called sup-21.

Shrinker-contracts both dorsally and ventrally when prodded. Slow, poor backing. Slightly small. M-MATING++ 1-10%WT

High Tc1 copy number, arose spontaneously in CB30. Him. Small, roundheaded.

Temperature sensitive. Sterile at 25C. Maintain at 15C. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. [2/98: Craig Mello noticed a different embryonic phenotype in this strain as compared to the e2141 stock that Jim Priess obtained from England-the ABp fate appears WT.] [NOTE (11/16/10 - J. Hubbard): This strain is NOT synonymous with glp-1(e2144) as previously reported in Kodoyianni V, Maine EM, Kimble J. (1992) [Molecular basis of loss-of-function mutations in the glp-1 gene of Caenorhabditis elegans. Mol Biol Cell. 3,1199-213. PMID: 1457827]. As reported in Worm Breeders Gazette December 2010; 18(3), e2144 carries the mutation c2785t in exon 8, leading to the amino acid change L929F, whereas e2141 carries the mutations c2920t and a3610g in exon 8, leading to the amino acid changes R974C and T1204A.]

Long, especially obvious in adults. Males mate well.

Wild-type hermaphrodites segregating WT, Fem, Unc Mor. Maintain by picking wild-type hermaphrodites. Deletion allele of fem-1, with unusual maternal effect. Reference: Spence AM, et al. Cell. 1990 Mar 23;60(6):981-90. Johnson CL & Spence AM.. Science. 2011 Sep 2;333(6047):1311-4.

Paralyzed Unc. This strain carries a tightly linked mutation, che-3(ky1018), that changes Q1107 of che-3 to a stop codon. Reference: Larsch, et al. (2015) Cell Reports.

Heterozygotes mostly fertile at or below 20C; all sterile at 25C. Progeny are fertile heterozygotes with variable Gon abnormality, e1254 homozygotes (strong Gon, "white patch" phenotype) and eDf18 homozygotes (embryonic lethal). See also WBPaper00003841.

Dpy hermaphrodites. Protruding vulva in adult; viable at all temperatures.

Dpy hermaphrodites. Protruding vulva in adult; viable at all temperatures.

y1 is a temperature sensitive mutation affecting only XX animals. him-8(e1489) causes X non-disjunction, resulting in many males. Grow at 15C. XX and XXX animals not viable above 15C. After shifting to 25C, surviving population is almost all XO males.

Wild-type hermaphrodites segregating wild-type hermaphrodites, Unc-24 females, Unc Dpy hermaphrodites. Maintain by picking wild-type hermaphrodites. Deletion allele of fem-1, with unusual maternal effect. Reference: Spence AM, et al. Cell. 1990 Mar 23;60(6):981-90. Johnson CL & Spence AM.. Science. 2011 Sep 2;333(6047):1311-4.

Small, severely uncoordinated. Spontaneous segregant from N2/RW7000 (Bristol/Bergerac) hybrid strain. Daf-d.

Hermaphrodites are WT at all stages. Adult males abnormal with swollen distorted bursal anatomy. Male mating efficiency is zero. Natural mutation present in original KR314 isolate. For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Medium coiler Unc at all stages.

Heterozygotes are WT and segregate WT, Lethals (L2/L3 arrest at 20C, some survive to sterile adults at 15C), and DpyRol. Not balanced well: get recombinants. Must maintain by picking hets at each generation and check for proper segregants in F1.

Small. Recessive. Males abnormal-abnormal spicules. M-MATING-NO SUCCESS.