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Unbalanced two-fold arrest lethal. Clone 20 - 30 WT, screen F1 for presence of mup-1 homozygotes. Easily scored, they look like tiny little croissants.

Unbalanced two-fold arrest lethal. Clone 20 - 30 WT, screen F1 for presence of mup-1 homozygotes. Easily scored, they look like tiny little croissants.

Unbalanced two-fold arrest lethal. Clone 20 - 30 WT, screen F1 for presence of mup-1 homozygotes. Easily scored, they look like tiny little croissants.

mup-1 is a two-fold arrest lethal, suppressed in this strain by dpy-10.

mup-1 is a two-fold arrest lethal, suppressed in this strain by dpy-10.

mup-1(e2430), a ts two-fold arrest lethal, is suppressed by dpy-10.

mup-1(e2430) is a temperature sensitive two-fold arrest lethal. Maintain at 15C. Easily scored, at restrictive temperature they look like tiny little croissants. Muv and Unc.

Small, severely uncoordinated. Spontaneous segregant from N2/RW7000 (Bristol/Bergerac) hybrid strain. Daf-d.

NOTE: Whole-genome analysis indicates that this stock is genotypically CB4858. Users interested in this strain are encouraged to obtain a verified CB4855-derivied strain. To obtain ECA247, a sequenced isolate of this wild strain, or other whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org). Isolated from compost in Palo Alto, CA in 1982(?). Wild type (plg-1(e2001)). Low copy Tc1, pattern VI. Caenorhabditis elegans wild isolate CB subclone of Sta-5 (Tc1 pattern VI). Original stock isolated by T. Doniach.

Medium coiler Unc at all stages.

Maternally rescued. Homozygous e2519 worms coming from homozygous mothers develop slowly. Adult rhythmic behaviors are also slowed.

Heterozygotes are WT and segregate WT, Lethals (L2/L3 arrest at 20C, some survive to sterile adults at 15C), and DpyRol. Not balanced well: get recombinants. Must maintain by picking hets at each generation and check for proper segregants in F1.

Intersexual self-fertile XX hermaphrodites with Smg (protruding vulva) phenotype. Grows poorly, does best at 20C. Class B1 allele of tra-1 combined with smg-2(NMD), useful for selection of modifiers. Reference: Zarkower et al. (1994) PMID: 7520378

Hypomorphic allele. Homozygous XX animals are masculinized; have hermaphrodite gonad, abnormal male non-gonadal anatomy; self-fertile.

At 20C or 25C heterozygotes are semi-Dpy and segregate semi-Dpys (heterozygotes), Dpys (dpy-25/dpy-25) and animals which arrest as Dpyish young larvae (let-552/let-552). Maintain by picking semi-Dpy. At 15C it is difficult to distinguish between let-552 homozygotes and dpy-25 homozygotes (e817 is inviable at 15C).

Slow growth and movement. Egl. Somewhat Uncoordinated. Maternal effect: m+z- homozygotes have no obvious phenotype.

Obligate XO male/female strain, propagate by crossing. Low fertility. Fertile XO females and XO males; inviable XX embryos and larvae. Reference: Strain 7 Hodgkin (2002) PMID: 12399387.

Slow growth. Maternal rescue: m+z- homozygotes have no obvious phenotype.

Hermaphrodites have abnormal truncated or blebbed tails, pale appearance, some excretory cell abnormalities, especially in adults. Males have variably abnormal tails, some are able to mate.

Heterozygotes are Unc and segregate Uncs, dead eggs, and WT (dif-1/dif-1). The WT animals give 100% dead eggs. dif-1 is a maternal effect embryonic lethal gene and e2562 is a putative null allele.

Heterozygous. Maintain by picking fertile hermaphrodites. Stable XX male/female strain (fertile XX females and low fertility XX males); males sire only female progeny. Reference: Strain 3 in Hodgkin (2002) PMID: 12399387.

Self-fertile hermaprodite or female. Protruding vulva. tra-1(e2272) XX homozygotes are sterile with abnormal gonads; e2272 mutation is partly suppressed by NMD (smg-2(e2008)), hence double mutants are fertile. Reference: Zarkower et al. (1994) PMID: 7520378.

Hermaphrodites are WT. Males form copulatory plug when mating. Weaker than CB3855 males.

Intragenic partial revertant of tra-1(e2272). Fertile hermaphrodites or females. Reference: Zarkower et al. (1994) PMID: 7520378.

Severely uncoordinated coiler, slow growing. Useful strain for selecting non-Sup-1 suppressors of unc-17(e245). Reference: Mathews et al. (2012) PMID: 23051648.

WT hermaphrodites segregate WT hermaphrodites and males, Dpy hermaphrodites and males and Stu (Sterile and Uncoordinated) hermaphrodites and males.

Partial intragenic revertant. Weak Egl phenotype, less severe than egl-26(e1952). Reference: Hodgkin (1986) PMID: 3770465.

Small, blunt rounded nose; heterozygous e2656/+ are animals similar, XO males can mate. Molecularly and phenotypically different from sma-8(e2111).

Unable to propagate indefinitely: lines become sterile from F10-F28, with short telomeres and fused chromosomes. Hypersensitive to X-irradiation; weak Him phenotype; strong Him phenotype in later generations resulting from X-A fusions. Cross once or twice, freeze down many F2 mrt-2 plates, and go back to these plates every two months for fresh a mrt-2 line. A BstN1 RFLP makes the mrt-2 mutation easy to track.

let-560(e2658) was a spontaneous mutation in strain DH424. let-560 homozygotes are late embryonic lethal. Strain segregates WT, Vul and dead eggs.

fox-1(e2643) is a 1.2 kb deletion of fox-1; functional null. Hermaphrodites and males are WT in gross phenotype, slightly abnormal in hermaphrodite fertility and male mating. Synergistic masculinizing effects with some X chromosome deletions.

Him. Sterile at 25C. Weak allele, R862stop.

Resistant to infection by Microbacterium nematophilum (no tail swelling). Slightly small.

Dumpy. e2760/0 Dpy males are able to mate (ME 1/2), in contrast to other dpy-6 alleles. mrt-2 induced.

Resistant to infection by Microbacterium nematophilum (no tail swelling).

Resistant to infection by Microbacterium nematophilum (no tail swelling).

Resistant to infection by Microbacterium nematophilum (no tail swelling).

Resistant to infection by Microbacterium nematophilum (no tail swelling). Slightly skiddy movement, some cuticle fragility.

Resistant to infection by Microbacterium nematophilum (no tail swelling).

Bus (M. nematophilum resistant), bleach-sensitive, non-Bah (Yersinia sensitive). e2701 is an intermediate strength missense allele, S167N (YAASKAA>YAANKAA). Reference: O'Rourke et al (in preparation).

Resistant to infection by Microbacterium nematophilum (no tail swelling).

Resistant to infection by Microbacterium nematophilum (no tail swelling). Abnormal lectin staining. ptr-15 also known as bus-13.

Resistant to infection by Microbacterium nematophilum (no tail swelling).

Male/hermaphrodite strain, propagate by crossing. Fertile XX hermaphrodites and fertile XX males. Obligate XX strain with sex determined by amber-suppressing tRNA, sup-34. Reference: Strain 14 in Hodgkin (2002) PMID: 12399387.

Surface abnormal, resistant to infection by Microbacterium nematophilum. Reference: Gravato-Nobre et al. (2005) PMID: 16079230.

Dumpy (partially-suppressed Dpy-31). Sqt-3 mediated suppression of dpy-31 lethality. Reference: Novelli et al. (2004) PMID: 15579684.

Resistant to infection by Microbacterium nematophilum (no tail swelling). Skiddy movement; frequent vulval rupture; bleach sensitive; hypersensitive to drugs. Abnormal lectin staining.

Abnormal response to infection by Microbacterium nematophilum (no tail swelling). Abnormal lectin staining.

Him. Sterile at 25C. Weak allele, M223I.

Resistant to infection by Microbacterium nematophilum (no tail swelling). Small, skiddy movement; slow-growing; frequent vulval rupture; bleach sensitive, hypersensitive to drugs. Abnormal lectin staining.