More Fields
Strain Species Genotype
RM3571 C. elegans sup-1(e995 e2636) III. Show Description
Putative null allele; e995 e2636 homozygotes are superficially wild type in appearance, development, and behavior (except for a modest 20-25% decrease in swimming rate), and do not suppress unc-17(e245). e995 corresponds to G84E (gga>>gaa) and e2636 corresponds to W58stop (tgg>>tag). PCR methods for scoring e995 and e2636 mutations in individual worms are presented in the Supporting Information File of Mathews et al., 2012. Reference: Mathews EA, et al. Genetics. 2012 Dec;192(4):1315-25.
RM3670 C. elegans sup-1(e995) III. Show Description
e995 homozygotes are superficially wild type in appearance, development, and behavior. e995 is a strong, dominant suppressor of UNC-17 G347R mutations (including e245, e359, p300). e995 corresponds to G84E (gga>>gaa) in the SUP-1 transmembrane domain. PCR method for scoring the e995 mutation in individual worms is presented in the Supporting Information File of Mathews et al., 2012. [Note: although it has been suggested that unc-123 and sup-1 represent the same gene (Walthall et al. 1993), sequence analysis demonstrated no molecular lesions at the sup-1 locus in unc-123 mutants (Mathews et al., 2012). Therefore unc-123 and sup-1 represent different genes.] Reference: Mathews EA, et al. Genetics. 2012 Dec;192(4):1315-25.
KG4731 C. elegans unc-116(ce815[LoxP+sup-1(e995)+LoxP]) III. Show Description
Lox P sites in 3' UTR and 4th intron flank kinesin motor domain sequences; sup-1(e995) mini-gene inserted in second intron. Appears wild-type on plates and in quantitative locomotion assays. Can be used to conditionally delete gene sequences encoding the conventional kinesin motor domain in a tissue specific manner by driving expression of Cre recombinase in the tissue of interest. Reference: Stec N, et al. (Submitted). An Intron Compatible Marker for Long Distance CRISPR Mediated Gene Editing in Caenorhabditis elegans.
KG5148 C. elegans unc-104(ce833[5xMyc::AID::unc-104+sup-1(e995)]) II. Show Description
The Auxin Inducible Degron (AID) flanked by a 5X Myc/spacer tag on left and a single spacer on the right is fused to the N-terminus of the unc-104 gene. Allows conditional degradation of UNC-104 protein when combined with tissue specific expression of TIR1 in the presence of 1 mM Auxin in plate media. Note: KG5148 does not express TIR1. On standard plates: wild type growth, appearance, and locomotion rate. For animals expressing a TIR1 transgene in the nervous system: Animals that hatch and develop on 1 mM Auxin plates generally remain tightly coiled near the location of hatching and exhibit slow growth (up to 7 days to reach adulthood, with 98% reaching adulthood by 7 days). Adults placed on Auxin abruptly lose about 75% of their locomotion function between 6 and 12 hours after plating. The remaining locomotion function is lost gradually between 12 and 52 hours. Reference: Stec N, et al. (Submitted). An Intron Compatible Marker for Long Distance CRISPR Mediated Gene Editing in Caenorhabditis elegans.
CB2108 C. elegans dpy-18(e364) sup-1(e995) III. Show Description
Dpyish/Suppressed. Variable expression.
RM3643 C. elegans sup-1(e995 e2636) III; unc-17(e245) IV. Show Description
Indistinguishable from unc-17(e245) single mutants (small, slow-growing, coily uncoordinated, jerky going backward, aldicarb-resistant). For additional information, see descriptions of the RM908 unc-17(e245) IV and RM3671 sup-1(e995 e2636)) III single mutant strains. PCR methods for scoring e245, e995, and e2636 mutations in individual worms are presented in the Supporting Information File of Mathews et al., 2012. Derived by crossing RM908 (6x outcrossed) and RM3571 (6x outcrossed). Reference: Mathews EA, et al. Genetics. 2012 Dec;192(4):1315-25.
RM3660 C. elegans sup-1(e995) III; unc-17(e245) IV. Show Description
Almost full suppression of all unc-17 mutant phenotypes; animals are superficially wild type in appearance, development, and behavior, and males mate well. For additional information, see descriptions of the RM908 unc-17(e245) IV and the RM3670 sup-1(e995) III single mutant strains. PCR methods for scoring e245 and e995 mutations in individual worms are presented in the Supporting Information File of Mathews et al., 2012. Reference: Mathews EA, et al. Genetics. 2012 Dec;192(4):1315-25.
CB5265 C. elegans sup-1(e995e2636) III; unc-17(e245) IV; xol-1(y9) X. Show Description
Severely uncoordinated coiler, slow growing. Useful strain for selecting non-Sup-1 suppressors of unc-17(e245). Reference: Mathews et al. (2012) PMID: 23051648.
CB933 C. elegans unc-17(e245) IV. Show Description
M-MATING-NO SUCCESS. UNC-Severe coiler at all stages-small and thin. SCORED EASILY. Suppressed by sup-1, sup-2, and snb-1. Resistant to lannate. See also CGC 1770.
ER10 C. elegans unc-123(jd5) III. Show Description
Temperature sensitive, semidominant mutant that is Unc when moving backward. Dorsal muscular contraction is stronger than that of ventral, resulting in an asymmetric pattern of locomotion in which the animal either forms a dorsal coil or moves in circles with the dorsal side central to the circle. Most likely a gain of function allele of sup-1.
ER50 C. elegans unc-123(jd5jd10) III. Show Description
Recessive suppressor of unc-123(jd5). Exhibits a subtle dorsal asymmetric locomotory defect as a homozygote when grown at 25C. Dominant suppressor of unc-17(e245). Most likely unc-123 is allelic with sup-1.
DR605 C. elegans unc-15(e73) I; sup-19(m210) V. Show Description
Unc phenotype weakly suppressed. Very slow.
JK1009 C. elegans fog-1(q180)/sup-11(n403) unc-11(e47) I. Show Description
Heterozygotes are WT and segregate WT, small scrawny slow-growing Uncs, and females. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JT5218 C. elegans sup-17(sa38) I; lin-12(n302) III. Show Description
sa38 is a weak Egl and a moderate suppressor of lin-12(gf).
JT5222 C. elegans sup-19(sa45) I; lin-12(n302) III. Show Description
sa45 is a weak Egl and a moderate suppressor of lin-12(gf).
MT1122 C. elegans sup-11(n403) I; unc-93(e1500) III. Show Description
Phenotype: small, scrawny, thin, lays few eggs. unc-93(e1500) rubberband phenotype is completely suppressed by sup-11(n403) so only sup-11 phenotype is visible. n403 is semidominant.
MT1132 C. elegans unc-93(e1500) sup-18(n463) III. Show Description
MT15080 C. elegans sup-17(n1306) unc-29(e1072) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
Heterozygotes are WT and GFP+. hT2[qIs48] animals are recessive lethal. n1306 is recessive late larval lethal. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype.
MT15081 C. elegans sup-17(n1315) unc-29(e1072) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
Heterozygotes are WT and GFP+. hT2[qIs48] animals are recessive lethal. n1315 is recessive lethal. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype.
MT15082 C. elegans sup-17(n1318) unc-29(e1072) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
Heterozygotes are WT and GFP+. hT2[qIs48] animals are recessive lethal. n1318 is recessive lethal. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype.
MT15083 C. elegans sup-17(n1319) unc-29(e1072) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
Heterozygotes are WT and GFP+. hT2[qIs48] animals are recessive lethal. n1319 is recessive lethal. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype.
MT15084 C. elegans sup-17(n1320) unc-29(e1072) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
Heterozygotes are WT and GFP+. hT2[qIs48] animals are recessive lethal. n1320 is recessive lethal. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype.
MT1789 C. elegans sup-17(n316) I. Show Description
Dpy. Egl. Abnormal vulval cell lineages.
MT2056 C. elegans sup-10(n983) X. Show Description
Class 3 (neomorphic) allele. Uncoordinated, rubberband paralysis like unc-93(e1500). Suppressed by intragenic revertants and unc-93 alleles.
MT2769 C. elegans sup-17(n1258) I. Show Description
sup-17(n1258) is a recessive suppressor of lin-12(n302sd).
MT2773 C. elegans sup-17(n1260) I. Show Description
Temperature sensitive. Sup Vul. Egl of n302.
MT2941 C. elegans sup-17(n1305) unc-29(e1072) I. Show Description
ts. Dpy. Egl. sup of lin-12(d).
MT2966 C. elegans sup-17(n1313) unc-29(e1072) I. Show Description
Suppressor of lin-12(d).
MT2967 C. elegans sup-17(n1314) unc-29(e1072) I. Show Description
Suppressor of lin-12(d).
MT2969 C. elegans sup-17(n1316) unc-29(e1072)/dpy-14(e188) I. Show Description
Heterozygotes are WT and segregate WT, Dpys and very slow growing Uncs. Maintain by picking WT.
MT2970 C. elegans sup-17(n1317) unc-29(e1072) I. Show Description
Suppressor of lin-12(d).
MT3497 C. elegans dpy-17(e164) sup-18(n1014) III; sup-10(n983) X. Show Description
Animals are Dpy non-Unc.
MT3589 C. elegans sup-9(n1435) lin-31(n301) II; sup-10(n983) X. Show Description
Non-rubberband. Muv.
MT3807 C. elegans lin-17(n671) sup-11(n403) I. Show Description
Bivulva. n403 is homozygous scrawny.
MT3964 C. elegans sup-9(n1550) II; lin-15B&lin-15A(n765) sup-10(e2127) X. Show Description
n765 is temperature-sensitive Muv. Loss-of-function of sup-10(e2127) suppresses the sup-9(n1550) Rubberband mutation.
MT5519 C. elegans sup-9(n1550n2174) II; sup-10(n983) X. Show Description
Weak Rubberband.
MT5896 C. elegans sup-9(n1550) II; unc-93(n2289) sup-18(n1014) III. Show Description
MT6015 C. elegans unc-93(n2334) dpy-17(e164) III; sup-10(n983) X. Show Description
n2334 suppresses n983. Dpy.
MT6322 C. elegans sup-11(n710) I; sup-9(n1550) II. Show Description
Unc and Egl. Grows slowly.
MT7026 C. elegans mek-2(n2679)/sup-11(n403) dpy-5(e61) I. Show Description
Heterozygotes are WT and segregate WT, steriles with a vulval defect, and scrawny Dpys. n2679 is a suppressor of let-60(n1046) Muv, and is recessive sterile with vulval defects. n267 is an intermediate strength allele. See also WBPaper00002150.
MT725 C. elegans unc-93(e1500) III; sup-10(n183) X. Show Description
Non-Unc.
MT8667 C. elegans mek-2(n2678)/sup-11(n403) dpy-5(e61) I. Show Description
Heterozygotes are WT and segregate WT, Sterile Vuls and scrawny Dpys. n2678 is a suppressor of let-60(n1046) Muv. n2678 animals are Vul and recessive sterile. n2678 is a strong allele, probably null. See also WBPaper00002150.
MT912 C. elegans sup-11(n403) I. Show Description
Dominant suppressor of unc-93(e1500), recessive small scrawny slow growing phenotype.
RW2306 C. elegans unc-60(e677) V; sup-12(st89) X. Show Description
Hermaphrodite ovaries are abnormal in morphology and accumulate many mature but unfertilized oocytes.
RW2337 C. elegans sup-13(st210) III; unc-78(e1217) X. Show Description
WT strain.
SP797 C. elegans unc-3(e151) osm-1(p808) X; mnDp13 (X;f). Show Description
WT phenotype. Segregates Uncs which are Osm. [Osm phenotype checked 2/94.] mnDp3[sup-10(mn338)] and mnDp13 are the same. mnDp13 was derived from mnDp3 as lacking sup-10(+). It is not known if mnDp3 picked up a mutation in sup-10 or if the end of mnDp3 was shortened.
TY788 C. elegans lin-15B&lin-15A(n765) sup-10(n983) X. Show Description
Animals are Muv and Unc at 20C and 25C. At 15C the animals are Unc. Temperature sensitive Muv phenotype. See also WBPaper00001990.
VC1595 C. elegans +/szT1 [lon-2(e678)] I; sup-12(ok1843)/szT1 X. Show Description
T22B2.4. Apparent homozygous lethal deletion chromosome balanced by lon-2-marked translocation. Heterozygotes are WT, and segregate WT, Lon-2 males, arrested szT1 aneuploids, and ok1843 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: TGCAGGTCAATTTCACGGTA. External right primer: CAATGAGGATGTGCAAATGG. Internal left primer: AATGTACGGCCAAGTCCAAG. Internal right primer: TATTGCTGGGACGACAATGA. Internal WT amplicon: 2626 bp. Deletion size: 1803 bp. Deletion left flank: CGCCGAACCAGTAGTTGGTGAGTTTCCTGT. Deletion right flank: CAATGATTTTACTTTTCATTCTATCCAAAT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
XE1026 C. elegans sup-17(n316); oxIs12. Show Description
oxIs12 [unc-47p::GFP] X. GFP expression in GABA neurons. Neuron. 2012 Jan 26;73(2):268-78.
XE1065 C. elegans sup-17(n316) I; oxIs12 X ; wpEx16. Show Description
oxIs12 [unc-47p::GFP] X. GFP expression in GABA neurons. wpEx16 [unc-47p::sup-17 + myo-2p::GFP]. Pick animals with GFP+ pharynx to maintain array. GABAergic expression of SUP-17 from wpEx16 rescues sup-17(n316). Reference: El Bejjani R, Hammarlund M. Neuron. 2012; 73(2):268-78.