| RW12196 |
C. elegans |
egrh-3(st12196[egrh-3::TY1::EGFP::3xFLAG]) IV. Show Description
egrh-3(st12196[egrh-3::TY1::EGFP::3xFLAG]) IV.
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| RW12212 |
C. elegans |
lin-1(st12212[lin-1::TY1::EGFP::3xFLAG]) IV. Show Description
lin-1(st12212[lin-1::TY1::EGFP::3xFLAG]) IV.
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| RW12221 |
C. elegans |
nhr-41(st12221[nhr-41::TY1::EGFP::3xFLAG]) IV. Show Description
CRISPR/Cas9 engineered tagged endogenous locus.
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| RW12239 |
C. elegans |
pes-1(st12239[pes-1::TY1::EGFP::3xFLAG]) IV. Show Description
CRISPR/Cas9 engineered tagged endogenous locus.
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| RW12249 |
C. elegans |
uaf-2(st12249[uaf-2::TY1::EGFP::3xFLAG]) IV. Show Description
CRISPR/Cas9 engineered tagged endogenous locus.
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| RW12252 |
C. elegans |
elg-18(st12252[elg-18::TY1::EGFP::3xFLAG]) IV. Show Description
CRISPR/Cas9 engineered tagged endogenous locus.
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| RW12295 |
C. elegans |
Y67H2A.10(st12295[Y67H2A.10::TY1::EGFP::3xFLAG]) IV. Show Description
CRISPR/Cas9 engineered tagged endogenous locus.
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| RW12304 |
C. elegans |
aptf-2(st12304[aptf-2::TY1::EGFP::3xFLAG]) IV. Show Description
eGFP and 3xFLAG tags inserted into endogenous locus by CRISPR/Cas9.
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| RW12314 |
C. elegans |
lag-1(st12314[lag-1::TY1::EGFP::3xFLAG]) IV. Show Description
CRISPR/Cas9 engineered tagged endogenous locus.
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| RW12324 |
C. elegans |
madf-9(st12324[madf-9::TY1::EGFP::3xFLAG]) IV. Show Description
CRISPR/Cas9 engineered tagged endogenous locus.
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| RW12332 |
C. elegans |
sta-1(st12332[sta-1::TY1::EGFP::3xFLAG]) IV. Show Description
CRISPR/Cas9 engineered tagged endogenous locus.
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| RW12337 |
C. elegans |
dnj-11(st12337[TY1::EGFP::3xFLAG::dnj-11]) IV. Show Description
CRISPR/Cas9 engineered tagged endogenous locus.
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| RW12339 |
C. elegans |
zip-7(st12339[TY1::EGFP::3xFLAG::zip-7]) IV. Show Description
CRISPR/Cas9 engineered tagged endogenous locus.
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| RW12345 |
C. elegans |
madf-1(st12345[madf-1::TY1::EGFP::3xFLAG]) IV. Show Description
CRISPR/Cas9 engineered tagged endogenous locus.
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| RW1324 |
C. elegans |
fem-1(e1991) unc-24(e138) unc-22(s12)/stDf7 IV. Show Description
Heterozygotes have Unc-24 phenotype and segregate additional hets, embryonic lethals (stDf7/stDf7) and Unc females (or sometimes hermaphrodites) that Twitch.
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| RW1333 |
C. elegans |
fem-1(e1991) unc-24(e138) unc-22(s12)/stDf8 IV. Show Description
Heterozygotes have Unc-24 phenotype and segregate additional hets, embryonic lethals (stDf8/stDf8) and Unc females (or sometimes hermaphrodites) that Twitch.
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| RW1350 |
C. elegans |
unc-44(e362) unc-82(e1323)/stDf7 IV. Show Description
Segregates heterozygotes that display only the unc-82 phenotype: slow but normal movement, disorganized muscle structure, somewhat Egl. stDf7 homozygotes die as embyros. unc-44 unc-82 homozygotes generally die as coiled, poorly-moving larvae.
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| RW20105 |
C. briggsae |
Cbr-unc-119(st20000) III; stIs20105 IV. Show Description
stIs20105 [Cbr-myo-2p::GFP + Cbr-unc-119(+)] IV. Transgene inserted into RW20000 Cbr-unc-119. Insertion site is between 5.64 and 12.05 Mb on Chromosome IV. Reference: Bi Y, et al. PLoS Genet. 2015 Feb 18;11(2):e1004993.
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| RW3562 |
C. elegans |
unc-44(e362) deb-1(st555)/unc-82(e1323) unc-24(e138) IV. Show Description
Heterozygotes are WT and segregate WT, Uncs (unc-82 unc-24 homozygotes) and dead eggs (unc-44 deb-1 homozygotes).
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| RW3563 |
C. elegans |
egl-19(st556)/unc-82(e1323) unc-24(e138) IV. Show Description
Heterozygotes are WT and segregate WT, Uncs, and Pats. Maintain by picking WT and checking for correct segregation of progeny. st556 pka pat-5.
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| RW3568 |
C. elegans |
pat-6(st561)/dpy-9(e12) IV. Show Description
Heterozygotes are WT and segregate WT, Dpys and Pats. Maintain by picking WT and checking for correct segregation of progeny.
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| RW3616 |
C. elegans |
deb-1(st554)/unc-5(e53) unc-24(e138) IV. Show Description
Heterozygotes are WT and segregate WT, Uncs and Pats. Maintain by picking WT and checking for correct segregation of progeny. Received new stock from Waterston lab 5/98. st554 previously called pat-8.
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| RW5020 |
C. elegans |
unc-22(s32) IV. Show Description
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| RW7080 |
C. elegans |
mut-4(st700) I; mut-5(st701) II; unc-22(st136) IV. Show Description
Uncoordinated Twitchers, moves slowly with constant twitching. Thin. Egl. Mutators give spontaneous reversion of unc-22.
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| RW7096 |
C. elegans |
mut-6(st702) unc-22(st192) IV. Show Description
Twitcher.
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| RW7097 |
C. elegans |
mut-6(st702) unc-22(st192st527) IV. Show Description
Mutator stock, carrying Tc1 induced revertant of a Tc1 induced unc-22 allele. Do not maintain by passaging.
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| SB133 |
Oscheius guentheri |
Oscheius guentheri wild isolate. Show Description
Wild-type reference strain for Oscheius guentheri (Sudhaus & Hooper, 1994). Isolated by D. J. Hooper in July, 1990, from decaying stem tissue of rice plants (Oryzae sativa, local cultivar Chet Som). Rice stems were sent to Hooper from R. Plowright (International Institute of Parasitology, St. Albans, Herts, England) and were originally collected by N. T. T. Cuc (Univ. of Can Tho) from An Brih, near Can Tho, Hau Giang Province, Vietnam. Hermaphroditic. Originally cultured on Nigon’s agar and agar with pieces of raw potato or meat, but grows on E. coli OP50 at 20-25˚C. Survives freezing using the C. elegans protocol. Synonymous strain designations: DF5032, PS2309. Sent to the CGC by David Fitch who obtained the strain from Walter Sudhaus Sept. 24, 1993.
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| SD366 |
C. elegans |
mpk-1(n2521) III; let-60(n1046) IV. Show Description
Less than 5% Muv animals.
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| SD39 |
C. elegans |
unc-30(e596) IV. Show Description
Unc. Allele specific suppression by smg-1(e1228), sup-5(e1464) and sup-7(st5) [in descending order of suppression].
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| SD551 |
C. elegans |
let-60(ga89) IV. Show Description
Temperature sensitive. Nearly WT at 15C. At 20C the animals are 18% Muv and brood size is 88. At 25C the animals are 57% Muv and almost sterile (brood size is 6). Maintain at 15C.
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| SHG1682 |
C. elegans |
elli-1(ust354[elli-1::GFP::3xFlag]) IV. Show Description
GFP::3xFlag inserted into endogenous elli-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans. Reference: Chen X, et al. Nat Commun. 2024 Jul 10;15(1):5799. doi: 10.1038/s41467-024-50027-3. PMID: 38987544.
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| SHG2028 |
C. elegans |
pgl-1(ust363[pgl-1::tagBFP]) IV. Show Description
tagBFP inserted into endogenous pgl-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans. Reference: Chen X, et al. Nat Commun. 2024 Jul 10;15(1):5799. doi: 10.1038/s41467-024-50027-3. PMID: 38987544.
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| SHG2040 |
C. elegans |
csr-1(ust371[3xFlag::GFP::csr-1]) IV. Show Description
3xFlag::GFP inserted into endogenous csr-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2240 |
C. elegans |
dcap-1(ust409[dcap-1::GFP::3xFlag]) IV. Show Description
GFP::3xFlag inserted into endogenous dcap-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2328 |
C. elegans |
plp-1(ust432[plp-1::GFP::3xFlag]) IV. Show Description
GFP::3xFlag inserted into endogenous plp-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2901 |
C. elegans |
csr-1(ust400[mCherry::csr-1]) IV. Show Description
mCherry inserted into endogenous csr-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHG2905 |
C. elegans |
pgl-1(ust588[pgl-1::GFP::3xFlag]) IV. Show Description
GFP::3xFlag inserted into endogenous pgl-1 locus using CRISPR/CAS9 engineering. Reference: Huang X, et al. 2024. Dev Cell. Compartmentalized localization of perinuclear proteins within germ granules in C. elegans.
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| SHX3908 |
C. elegans |
C42D4.3(zju273[C42D4.3::8xMS2]) IV; zjuEx2144. Show Description
8xMS2 tag inserted at C-terminus of endogenous C42D4.3 locus. zjuEx2144 [semo-1p::MCP::24xSuntag + col-19p::scFv::sfGFP + ttx-3p::RFP]. Pick ttx-3::RFP+ animals to maintain. MS2-based signal Amplification with Suntag System (MASS). Enhanced MS2-based single-molecule RNA imaging system utilizes Suntag signal amplifier. Suntag is a 19 amino acid protein tag that binds to its specific single-chain variable fragment (scFv) antibody. zjuEx2144 transgene expression provides MS2 coat protein (MCP) fused to 24xSuntag and scFv tagged with GFP. When all the required elements of the MASS (MS2, MCP, 24xSuntag, and scFv antibodies) are present, bright GFP foci will mark the tagged RNA. Reference: Hu Y, et al. eLife. 2023 Mar 3;12:e82178. doi: 10.7554/eLife.82178. PMID: 36867026.
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| SK4013 |
C. elegans |
zdIs13 IV. Show Description
zdIs13 [tph-1p::GFP] IV. Transcriptional tph-1 reporter. Reference: Lloret-Fernández et al. eLife 2018;7:e32785 DOI: 10.7554/eLife.32785. Clark SG & Chiu C. Development. 2003 Aug;130(16):3781-94.
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| SK85 |
C. elegans |
zag-1(zd85) IV. Show Description
Unc.
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| SK86 |
C. elegans |
zag-1(zd86) IV. Show Description
Unc.
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| SL1138 |
C. elegans |
spe-42(tn1231) V/nT1 [unc-?(n754) let-? qIs50] (IV;V). Show Description
Heterozygotes are Unc and express myo-2::GFP in the pharynx. spe-42(tn1231) homozygotes are non-Unc and non-GFP. spe-42(tn1231) produces <1 self progeny at 16C and no self progeny at 25C. The Spe defect can be rescued by WT sperm. Homozygous nT1[unc-?(n754) let-? qIs50] are embryonic lethal.
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| SL305 |
C. elegans |
spe-26(eb8) IV/nT1 [unc-?(n754) let-?] (IV;V). Show Description
Heterozygotes are Unc and segregate Unc and WT which are Spe (they lay only oocytes on the plate). Maintain by picking Unc.
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| SL753 |
C. elegans |
spe-39(tx12) V/nT1 [unc-?(n754) let-?] (IV;V). Show Description
Heterozygote are fertile and Unc. spe-39(tx12) homozygotes are self-sterile due to defects in spermatogenesis.
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| SL754 |
C. elegans |
spe-39(eb9) V/nT1 [unc-?(n754) let-?] (IV;V). Show Description
Heterozygote are fertile and Unc. spe-39(eb9) homozygotes are self-sterile due to defects in spermatogenesis.
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| SL958 |
C. elegans |
unc-42(e270) spe-42(eb5) V/nT1 [unc-?(n754) let-? qIs50] (IV;V). Show Description
Heterozygotes are Unc and express myo-2::GFP in the pharynx. unc-42(e270) spe-42(eb5) homozygotes are Unc and non-GFP. Whle unc-42(e270) spe-42(eb5) self progeny have not been quantified, spe-42(eb5) alone produces 9 self progeny at 16C and <1 self progeny at 25C. The Spe defect can be rescued by WT sperm. Homozygous nT1[unc-?(n754) let-? qIs50] are embryonic lethal.
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| SLR158 |
C. elegans |
pmk-3(tm745) IV; dvIs67; stxEx12. Show Description
dvIs67 [tbb-6p::GFP + myo-3p::dsRed]. stxEx12 [eft-3p::pmk-3 S(EE)::SL2::mCherry]. Pick animals with mCherry expression in intestinal cells. Reference: Munkacsy E, et al. PLoS Genet. 2016 Jul 15;12(7):e1006133.
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| SLR246 |
C. elegans |
unc-119(ed3) III; stxEx39. Show Description
stxEx39 [alx-1::TY1::eGFP::3xFLAG + Cbr-unc-119(+)]. Pick non-Unc to maintain. Constitutive expression of GFP. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of alx-1 coding sequence in fosmid WRM064D_F05 by recombineering (TransgeneOme bacterial clone 095794875301489 F02). Reference: Radetskaya O, et al. The PMK-3 (p38) Mitochondrial Retrograde Response Functions in Intestinal Cells to Extend Life via the ESCRT Machinery. bioRxiv 797308; doi: https://doi.org/10.1101/797308
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| SLR247 |
C. elegans |
unc-119(ed3) III; stxEx48. Show Description
stxEx48 [C01A2.4::TY1::eGFP::3xFLAG + Cbr-unc-119(+)]. Pick non-Unc to maintain. Constitutive expression of GFP. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of C01A2.4 coding sequence in fosmid WRM061C_F01 by recombineering (TransgeneOme bacterial clone 7225558184899882 D12). Reference: Radetskaya O, et al. The PMK-3 (p38) Mitochondrial Retrograde Response Functions in Intestinal Cells to Extend Life via the ESCRT Machinery. bioRxiv 797308; doi: https://doi.org/10.1101/797308
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| SLR248 |
C. elegans |
unc-119(ed3) III; stxEx35. Show Description
stxEx35 [istr-1::TY1::eGFP::3xFLAG + Cbr-unc-119(+)]. Pick non-Unc to maintain. Constitutive expression of GFP. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of istr-1 coding sequence in fosmid WRM0636D_F01 by recombineering (TransgeneOme bacterial clone 5438954842362824 D10). Reference: Radetskaya O, et al. The PMK-3 (p38) Mitochondrial Retrograde Response Functions in Intestinal Cells to Extend Life via the ESCRT Machinery. bioRxiv 797308; doi: https://doi.org/10.1101/797308
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