Strain Information

Name SHX3908   View On Wormbase
Species C. elegans
GenotypeC42D4.3(zju273[C42D4.3::8×MS2]) IV; zjuEx2144.
Description8xMS2 tag inserted at C-terminus of endogenous C42D4.3 locus. zjuEx2144 [semo-1p::MCP::24×Suntag + col-19p::scFv::sfGFP + ttx-3p::RFP]. Pick ttx-3::RFP+ animals to maintain. MS2-based signal Amplification with Suntag System (MASS). Enhanced MS2-based single-molecule RNA imaging system utilizes Suntag signal amplifier. Suntag is a 19 amino acid protein tag that binds to its specific single-chain variable fragment (scFv) antibody. zjuEx2144 transgene expression provides MS2 coat protein (MCP) fused to 24xSuntag and scFv tagged with GFP. When all the required elements of the MASS (MS2, MCP, 24xSuntag, and scFv antibodies) are present, bright GFP foci will mark the tagged RNA. Reference: Hu Y, et al. eLife. 2023 Mar 3;12:e82178. doi: 10.7554/eLife.82178. PMID: 36867026.
Made byJingxiu Xu
Laboratory SHX
Reference Yucen Hu # 1, Jingxiu Xu # 2, Erqing Gao # 2, Xueyuan Fan 1, Jieli Wei 1, Bingcheng Ye 1, Suhong Xu 2 3, Weirui Ma 1, Enhanced single RNA imaging reveals dynamic gene expression in live animals, Elife. 2023 Mar 3;12:e82178. doi: 10.7554/eLife.82178.
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