Search Strains

Use as a replacement strain for SP457. Rubberband Unc.

nIs396 [sams-5 3'::4xNLS-GFP + lin-15(+)] V. GFP expression in MI. Reference: Nakano S, et al. Development. 2010 Dec;137(23):4017-27

nIs407 [hlh-2::GFP + lin-15(+)]. Reference: Nakano S, Ellis RE, Horvitz HR. Development. 2010 Dec;137(23):4017-27.

nIs394 [ngn-1::GFP + lin-15(+)] III. Translational GFP reporter. Reference: Nakano S, et al. Development. 2010 Dec;137(23):4017-27.

nIs408 [lin-29p::lin-29::mCherry + ttx-3p::GFP] I. Reference: Harris DT, Horvitz HR. Development. 2011 Sep;138(18):4051-62.

Heterozygotes are Unc and segregate Unc, DpyUnc (Dpy is ts) and dead eggs. Maintain by picking Uncs.

Reference: Nakano S, et al. Cell. 2011 Dec 23;147(7):1525-36.

nIs281 [myo-2::RFP] integrated near qC1. Recombination between nIs281 and qC1 has been reported. Fails to complemement all markers on qC1. Heterozygotes are WT. Segregates Dpy Sterile and Dpy Unc.

Heterozygotes are WT GFP+. Segregates GFP+ Dpy Sterile and non-GFP Dpy Unc. nIs189 [myo-2::GFP] integrated in or near qC1. No recombination seen between nIs189 and qC1; fails to complement all markers on qC1.

nIs190 [myo-2::GFP] integrated in or near mnC1. Approx 0.5% recombination seen between nIs190 and mnC1. Fails to complemement all markers on mnC1. Heterozygotes are WT. Segregates WT GFP+ and Egl Unc Rol; no Dpy Uncs are seen as nIs190 mnC1 homozygotes are embryonic lethal.

nIs190 [myo-2::GFP] integrated in or near mnC1. Approx 0.5% recombination seen between nIs190 and mnC1. Fails to complemement all markers on mnC1. Heterozygotes are WT. Segregates WT GFP+ and Egl Unc Bli; no Dpy Uncs are seen as nIs190 mnC1 homozygotes are embryonic lethal.

nIs267 [myo-2::GFP] integrated in or near eT1. Unc.

nIs408 [lin-29p::lin-29::mCherry + ttx-3p::GFP] I. nIs454 [mab-10p::mab-10::GFP + ttx-3p::GFP] II. Reference: Harris DT, Horvitz HR. Development. 2011 Sep;138(18):4051-62.

nIs471 [lgc-55::GFP + lin-15(+)]. GFP expression in GLR glia-like cells and head muscles. Reference: Ringstad N, et al. Science. 2009 Jul 3;325(5936):96-100.

Egl. Dominant allele. Reference: Genetics 121(4):703-21 (1989).

Heterozygotes are WT and segregate WT, Vul and dead eggs. Maintain by picking WT.

Biv. Temperature-sensitive amber allele. Reference: Genetics (1985) 110:17-72.

Heterozygotes are Vul and segregate Vul, UncVul and dead eggs. Maintain by picking Vul non-Unc.

nIs540 [pig-1p::GFP + rol-6(su1006)] X. Rollers. Reference: Hirose T, Horvitz HR. Nature. 2013 Aug 15;500(7462):354-8.

Heterozygotes are WT and segregate WT, UncVul and dead eggs. The UncVuls are small, kinky, paralyzed and vulvaless. Maintain by picking WT.

Defective locomotion in response to blue/UV light. Double mutant has almost no response to light. Reference: Bhatla N & Horvitz HR. Neuron. 2015 Feb 18;85(4):804-18.

Heterozygotes are WT and segregate WT, UncVul and dead eggs. UncVuls are small, kinky, paralyzed and vulvaless. Maintain by picking WT.

Hets are WT and segregate WT, dead eggs, and VulUnc. VulUncs are small, kinky, paralyzed and vulvaless. Maintain by picking WT.

nEx2065 [gur-3p::GFP + lin-15(+)]. Maintain by picking non-Muv. GFP expression in I2, I4, AVD and PVC. Reference: Bhatla N & Horvitz HR. Neuron. 2015 Feb 18;85(4):804-18. PMID: 25640076.

Egl. [10/02: This strain was previously listed as being sel-10(n1069) or egl-41(n1069); these were found to be incorrect and the mutation is now called egl-1(n4065). H. Schwartz comm.]

Dominant egg laying defective. Slightly Unc.

Egl. Semi-dominant allele. Reference: Genetics 121(4):703-21 (1989).

nEx2287 [egl-6Ap::gur-3 + lin-15(+)]. Pick non-Muv to maintain. Exposure to light induces egg-laying. Reference: Bhatla N & Horvitz HR. Neuron. 2015 Feb 18;85(4):804-18. doi: 10.1016/j.neuron.2014.12.061. PMID: 25640076.

nIs534 [odr-1p::GCaMP3 + lin-15(+)]. nEx2314 [odr-1p::gur-3 + ges-1p::GFP]. Pick animals with GFP expression in gut to maintain. odr-1p::gur-3 expression in AWC and AWB causes them to respond to light exposure. Reference: Bhatla N & Horvitz HR. Neuron. 2015 Feb 18;85(4):804-18. doi: 10.1016/j.neuron.2014.12.061. PMID: 25640076.

Cells that normally die survive. [3/02: A mutation that was not reported (nucleotide 1251 C-> T causing codon 80 ->ochre) was found by Tak Hung. It turns out the mutation was misannotated in the original paper (Development, 1992, 116:309). Bob Horvitz also confirmed the discovery.

Heterozygotes are WT (slightly Unc) and segregate WT, Unc-36 and dead eggs. Maintain by picking WT.

Animals heterozygous for the duplication are WT. Animals which have lost the duplication are Unc. Animals homozygous for the duplication are viable. They are small, sickly Egl worms which don't give rise to Uncs.

Heterozygotes are WT and segregate WT, SmaUnc and dead eggs. Maintain by picking WT.

Heterozygotes are Sma. At 25C, hets segregate Sma, SmaMuv (lin-8; sma-3 lin-37), and SmaUncMuvSte (lin-8; sma-3 lin-13 unc-36). At 15C, hets segregate Sma, SmaMuv (lin-8; sma-3 lin-37) and SmaUnc which give sterile F2 (lin-8; sma-3 lin-13 unc-36). n387 is a ts maternal effect mutation.

Low penetrance Egl. HSN migration defective.

Egl. HSNs are migration defective and defective in serotonin expression. See also WBPaper00003422.

Egl. Defective in sex muscles (missing).

Egl. Maintain under normal conditions. Reference: DeVore DL, et al. Cell. 1995 Nov 17;83(4):611-20.

Egl. Maintain under normal conditions. Reference: DeVore DL, et al. Cell. 1995 Nov 17;83(4):611-20.

Partially osmotic avoidance defective. Recessive. FITC filling normal.

Osm. FITC filling defective.

Osmotic avoidance defective. FITC fills most amphid cells.

Semidominant osmotic avoidance defective. FITC fills normally.

Osmotic avoidance defective. FITC fills normally. Previously called osm-13.

Low penetrance Egl. HSN migration defective.

Egl. HSNs are migration defective and defective in serotonin expression.

Dpy. Persistent cell corpses. Maternal effect. Recessive.

Persistent cell corpses. Maternal effect. Recessive.

Temperature sensitive Muv. Semi-dominant Dpy. Unc-moves backward better than forward; slight kinker in forward movement; larvae more severly Unc.