Search Strains

wdEx455 [acr-14::GFP + unc-119(+)]. Pick non-Unc to maintain.

wdEx457 [F39B2.8::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in pharyngeal muscles, posterior ventral cord motor neurons, tail neurons, and a few head neurons. Construct made by M. Vidal lab; candidate unc-37 target gene. F39B2.8 also known as gpr-158.

wdIs62 contains [ceh-12::GFP + unc-119(+)]. Superficially wild-type.

wdEx60 contains [acr-5::GFP + dpy-20(+)]. Maintain by picking non-Dpy.

wdIs51 [F49H12.4::GFP + unc-119(+)]; likely integrated in X. GFP expression in PVD.

wdIs52 [F49H12.4::GFP + unc-119(+)].

wdEx637 [dat-1::3xFLAG::pab-1 + unc-119(+)]. Pick non-Unc to maintain. Reference: Spencer WC, et al. Genome Res. 2011 Feb;21(2):325-41.

hdIs32 [glr-1::DsRed2] III. otEx239 [rig-3::GFP + pha-1(+)]. Maintain at 25C and pick GFP+ to ensure the array is retained. AVA neurons are marked with GFP and DsRed2. Can be used to isolate AVA by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/). Derived from parental strain OH4326. Reference: Hutter H. J Microsc. 2004 Aug;215(Pt 2):213-8. doi: 10.1111/j.0022-2720.2004.01367.x. PMID: 15315508.

hdIs32 [glr-1::DsRed2]. gvEx173 [opt-3::GFP + rol-6(su1006)]. Maintain by picking Rollers, which should be GFP+. hdIs32 might be prone to silencing.

wdIs6 [del-1::GFP + dpy-20(+)]. Embryonic GFP expression in SABVR, SABVL, VBs in mid-L2, VAs in mid-L3.

wdEx75 [acr-5::GFP + lin-15(+)]. Expressed in B-type motor neurons.

wdIs5 [unc-4::GFP + dpy-20(+)] III. Reference: Lickteig KM, et al. J Neurosci. 2001 Mar 15;21(6):2001-14.

wdEx848 [mec-4::mCherry + unc-119(+)]. Pick non-Unc to maintain. mCherry expression in all six touch neurons. Array generated by bombardment; ~90% transmission rate. Reference: Smith CJ, O'Brien T, et al. Neuron. 2013 July 24; 79.

Unc. Reference: Winnier AR, et al. Genes Dev. 1999 Nov 1;13(21):2774-86.

hdIs1 [unc-53p::GFP + rol-6(su1006)] X. ufIs26 [unc-4p::mCherry + lin-15(+)]. Rollers. DA neurons marked with unc-4::mCherry and unc-53::GFP. unc-53::GFP expression in DAs is dim during L1. Can be used to isolate DA neurons by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).

ynIs37 [flp-13::GFP] III. juIs223 [ttr-39p::mCherry + ttx-3p::GFP] IV. ttr-39::mCherry alone marks VD and DD neurons. ttx-3::GFP marks AIY neurons. Combined ttr-39::mCherry and flp-13::GFP co-expression marks DD neurons in the L2 (only a few mCherry+/GFP+ DD neurons will show up briefly in the L2 stage and gene silencing apparently dims the GFP marker over time). VD neurons can be identified by mCherry alone, no GFP. Derived by crossing parental strains CZ8332 and NY2037. Can be used to isolate VB neurons by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).

ufIs26 [unc-4p::mCherry + lin-15(+)] II. vsIs13 [lin-11::pes-10::GFP + lin-15(+)] IV. GFP expression in six VC neurons and posterior intestine. VC neurons are labeled with both GFP and mCherry; co-labeling in VCs1-5 is brightest during L4 stage. Derived by crossing parental strains NC2957 and LX959. Can be used to isolate VC neurons by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).

otIs458[ceh-63p::GFP + unc-119(+)]. GFP expression in DVC neurons. Can be used to isolate DVC by FACS. Derived by outcrossing parental strain OH11974 to remove him-5(e1490). Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).

ufIs26 [unc-4p::mCherry + lin-15(+)] II. otIs707 [bnc-1p(1.8kb)::GFP]. VB neurons are GFP+ only; VA and SABV have both mCherry and GFP. Can be used to isolate VB neurons by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).

ynIs37 [flp-13p::GFP] III. ufIs26 [unc-4p::mCherry + lin-15(+)]. I5 neurons are labeled with GFP and RFP. Can be used to isolate I5 by FACS. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).

wdIs20 [unc-4p::snb-1::GFP + dpy-20(+)]. Animals are Lon, likely due to dpy-20(+) overexpression. Punctate GFP expression observed in dorsal nerve cord, ventral nerve cord, VC4, and VC5. Reference: Lickteig KM, et al. J Neurosci. 2001 Mar 15;21(6):2001-14.

wdIs22 [acr-5p::YFP + rol-6(su1006)]. Integrated array derived from wdEx223. Maintain by picking Rollers.

wdEx257 [unc-4::3XFLAG::pab-1 + (pSV17) unc-119 minigene]. Animals exhibit slight forward movement defect. Good antibody staining witih anit-FLAG M2 antibody (Sigma). 100% penetrant array. Expressed in all unc-4 neurons. Ectopically expressed in at least one head neuron (dorsal to pharynx, between two bulbs).

wdEx258 [twk-30::GFP + myo-3::DsRed2)]. GFP expression in perinuclear motor neurons.

wdEx290 [acr-15::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in VNC, head neurons, and tail neurons. Construct made by M. Vidal lab; candidate unc-37 target gene.

wdEx329 [F09C3.2::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in ventral nerve cord. Construct made by M. Vidal lab; candidate unc-37 target gene.

wdEx345 [F55C12.4::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in VNC, head neurons, and tail neurons. Construct made by M. Vidal lab; candidate unc-37 target gene.

wdEx346 [tig-1::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in head neurons, tail neurons. head muscles, and vulva. Construct made by M. Vidal lab; candidate unc-37 target gene.

wdEx348 [C13G3.1::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in VNC, head neurons, and tail neurons. Construct made by M. Vidal lab; candidate unc-37 target gene.

wdEx351 [tsp-7::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in motor neurons. Construct made by M. Vidal lab; candidate unc-37 target gene.

wdEx359 [F29G6.2::GFP + unc-119(+)]. GFP+ seen in ventral nerve cord. Pick non-Unc worms that are GFP+.

wdEx381 [F55C12.4::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in VNC, head neurons, and tail neurons. Construct made by M. Vidal lab; candidate unc-37 target gene.

wdEx419 [acr-16::GP + rol-6(su1006)]. Rollers. Maintain by picking rollers. GFP expression detectable in body wall muscle and DB motor neurons.

wdEx451 [Y71D11A.5::GFP + rol-6(su1006))]. Pick rollers to maintain. GFP expression in VD, DD, and AS neurons, multiple head and tail neurons, and muscles.

mig-22(tk69): DTC migration defect and maternal effect embryonic lethal. Heterozygotes are WT with pharyngeal GFP signal. Homozygous hT2[bli-4 let-? qIs48] are inviable.

Distal tip cell migration defective. Growth delay. Protruding vulva.

DTC migration defect.

Distal tip cell migration defective.

DTC migration defect.

Distal tip cell migration defective. Growth delay. Protruding vulva.

Distal tip cell migration defective. Tc1 insertion mutant. Spontaneous mutant with ventral white patch phenotype.

DTC migration defect.

Distal tip cell migration defective.

DTC migration defect.

Distal tip cell, HSN migration defective.

Distal tip cell, HSN, left cc mother cell, QR(d) migration defective.

Distal tip cell migration defective.

Cell corpses persist as unengulfed, refractile dics. DTC mismigrates. Sequenced: point mutation R38>STOP, probably a null.

vlcEx588 [aak-2p::GFP::unc-54 3'UTR + rol-6(su1006)]. Not all transgenic animals roll well; pick GFP+ or Rollers to maintain. Reporter contains aak-2 enhancer sequence (+748, +1527) inserted into pDD95.75. Reference: Lloret-Fernández et al. eLife 2018;7:e32785 DOI: 10.7554/eLife.32785. PMID: 29553368.

vlcEx599 [ast-1p::GFP::unc-54 3'UTR + rol-6(su1006)]. Not all transgenic animals roll well; pick GFP+ or Rollers to maintain. Reporter contains ast-1 enhancer sequence (-2852, -1999) inserted into pDD95.75. Reference: Lloret-Fernández et al. eLife 2018;7:e32785 DOI: 10.7554/eLife.32785.