Search Strains

Homozygous sterile mutation balanced by GFP- and dpy-10-marked inversion. Heterozygotes are wild-type with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP ok308 homozygotes (mostly sterile or Mel, but can be slowly propagated at 20C). Pick WT dim GFP and check for correct segregation of progeny to maintain.

Homozygous sterile mutation balanced by GFP- and dpy-10-marked inversion. Heterozygotes are wild-type with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP nos-2 nos-1 homozygotes (segregate many dead embryos). Pick wild-type dim GFP and check for correct segregation of progeny to maintain. Reference: Hansen D, et al. Development. 2004 Jan;131(1):93-104.

Heterozygotes are WT and segregate WT, Steriles (hd43 homozygotes) and Dpy Uncs.

Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Maintain by picking WT.

Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Maintain by picking WT.

Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Pick wild-type heterozygotes to maintain. ct281 homozygotes show Nob phenotype: approximately 80% of homozygous embryos arrest at about the time of hatching with fairly normal anterior development but a severely deformed posterior with a variable knob-like shape; approximately 20% fail to enclose and do not hatch. ct281 is a 4.7kb deletion removing intron 5, exon 6, and the 3'UTR of the pal-1 gene. Reference: Edgar LG, et al. Dev Biol. 2001 Jan 1;229(1):71-88.

Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Pick wild-type heterozygotes to maintain. ct224 homozygotes show Nob phenotype: approximately 80% of homozygous embryos arrest at about the time of hatching with fairly normal anterior development but a severely deformed posterior with a variable knob-like shape; approximately 20% fail to enclose and do not hatch. ct224 is a 4.2kb deletion removing exon 1 through exon 6 of the pal-1 gene. Reference: Edgar LG, et al. Dev Biol. 2001 Jan 1;229(1):71-88.

WT hermaphrodites that segregate WT, Dpy Steriles and Nob larvae. The Nob phenotype results in 100% lethality. Maintain by picking WT.

ctIs33 [pal-1::GFP + rol-6(su1006)]. Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Pick wild-type heterozygotes to maintain. ct224 homozygotes show Nob phenotype: approximately 80% of homozygous embryos arrest at about the time of hatching with fairly normal anterior development but a severely deformed posterior with a variable knob-like shape; approximately 20% fail to enclose and do not hatch. ct224 is a 4.2kb deletion removing exon 1 through exon 6 of the pal-1 gene. ctIs33 carries a non-rescuing pal-1::GFP fusion containing ~7kb 5' of the SL1 splice site through part of exon 5 fused to GFP. GFP expression is primarily embryonic and limited to a few cells; not visible except at high magnification. Reference: Edgar LG, et al. Dev Biol. 2001 Jan 1;229(1):71-88.

May have unc-4(e120) mutation in background. svDp1 balances from pal-1 through unc-36 on III. svDp1 made by fusing array containing [unc-4(+) + sur-5::GFP] to sDp3(III;f).

Uncoordinated. May contain a weak daf-2 mutation (sa875). See Ailion and Thomas in Genetics 165: 127-144 2003.

Paralyzed Unc. Null allele.

Limp paralyzed Unc. Larvae move slightly better. Egl. Disorganized muscle structure. Suppressed by sup-5 and sup-7.

Slow moving Unc. Paramyosin abnormal.

Body muscle abnormal. Limp paralyzed or very sluggish; somewhat Egl; larvae more paralyzed. M-MATING-NO SUCCESS.

Slow moving Unc. Body Muscle abnormal. M-MATING-NO SUCCESS.

Slightly slow Unc. Movement almost wild-type. Body muscle abnormal. M-MATING++ 1-10%WT.

Chemosensory abnormal. Slightly small. Defective dauer formation. M-MATING NO SUCCESS.

Blebs on tail of adult hermaphrodite. Tail irregular, tail cuticle sometimes separated, tail spike sometimes truncated. Some animals have enlarged excretory canals.

Throws 11% males.

Paralyzed Unc. Null allele.

Temperature sensitive Dpy. Reproduction poor at 25C. M-MATING+ AT LOW TEMPERATURE ONLY.

Paralyzed Unc. Small.

Paralyzed Unc.

Unc. Slow moving.

6% XO self-progeny. Males sterile. M-MATING-NO SUCCESS.

Male gonad abnormal. Males sterile. 6% XO self-progeny. M-MATING-NO SUCCESS.

Unc-fainter: stops. M-MATING+++ 10-30%WT.

Temperature sensitive. Lineage abnormal. Multi-vulva. M-MATING+POOR <1%WT.

Small Dpy.

Dpy. Temperature sensitive.

Dpy. Temperature sensitive. Lethal cold sensitive. Male lethal. M-MATING++ 1-10%WT.

Unc-fainter: stops.

Touch insensitive; lethargic.

Medium Dpy.

Dpy. Coiler Unc. Closely linked. Doesn't grow at 25C.

Lineage abnormal. Recessive. Segregates males. Males abnormal.

Segregates males. Males abnormal.

Heterozygotes are WT and segregate WT, Dpy and males.

Heterozygotes are WT and segregate WT, DpyUnc and males. Maintain by picking WT hermaphrodites.

Heterozygotes are WT and segregate WT, Dpy and Vulvaless. Maintain by picking WT.

Heterozygotes are WT. Segregates Dpy. Segregates sterile Unc--Unc cannot back. Balanced well.

Heterozygotes are WT and segregate WT, DpyUnc and males. Maintain by picking WT.

Balances well. Transformer weak. Heterozygotes are WT and segregate WT, DpyUnc and males. Maintain by picking WT.

Dominant suppressor of Unc. Dpy. sup-6 is recessive lethal. Heterozygotes are Dpy non-Unc. Pick Dpy non-Unc to maintain.

Movement slow. Suppressed Unc. Revertant. Heterozygotes move slowly and segregate larval lethals (eDf1 eDp21 homozygotes), and paralyzed Uncs.

Suppressed Unc.

Lineage abnormal. Bursae abnormal. Segregates abnormal males. M-MATING-NO SUCCESS.

Bursae abnormal. Have slightly protruding vulva in adults. Segregates males with abnormal swollen bursae. M-MATING++ 1-10%WT.

Bursae abnormal. Vulva protruding. Segregates abnormal males. M-MATING++ 1-10%WT.