Strain Information
| Name | BW1927 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | pal-1(ct224)/qC1 [dpy-19(e1259) glp-1(q339)] III; ctIs33. |
| Description | ctIs33 [pal-1::GFP + rol-6(su1006)]. Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Pick wild-type heterozygotes to maintain. ct224 homozygotes show Nob phenotype: approximately 80% of homozygous embryos arrest at about the time of hatching with fairly normal anterior development but a severely deformed posterior with a variable knob-like shape; approximately 20% fail to enclose and do not hatch. ct224 is a 4.2kb deletion removing exon 1 through exon 6 of the pal-1 gene. ctIs33 carries a non-rescuing pal-1::GFP fusion containing ~7kb 5' of the SL1 splice site through part of exon 5 fused to GFP. GFP expression is primarily embryonic and limited to a few cells; not visible except at high magnification. Reference: Edgar LG, et al. Dev Biol. 2001 Jan 1;229(1):71-88. |
| Mutagen | TMP/UV |
| Outcrossed | x4 |
| Made by | Lois Edgar |
| Laboratory | BW |
| Reference | n/a |
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