Search Strains

Heterozygotes are Unc and segregate Uncs, dead eggs, and Pvul slow growing tm2610 homozygotes. Pick Unc to maintain and check for correct segregation of progeny. Reference: Bhalla N, et al. PLoS Genet. 2008 4(10) e1000235.

ieSi1 [htp-3p::GFP::him-8 + unc-119(+)] II. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. ieSi1 is prone to silencing; GFP might not be visible at lower magnifications. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Wynne DJ, et al. J Cell Biol. 2012 Jan 9;196(1):47-64.

mIs11 [myo-2p::GFP + pes-10p::GFP + F22B7.9::GFP]. Heterozygotes are wild-type with dim GFP signal in the pharynx. mIs11 homozygotes are wild-type with bright GFP in the pharynx. ieDf2 homozygotes (non-GFP) develop normally but produce 97.5% inviable embryos and a high frequency of males among the surviving self-progeny. Pick WT with dim GFP+ in pharynx to maintain. mIs11 homozygotes will quickly overtake the population if not selected against. GFP expression in 4-cell embryos, pharyngeal muscle and gut. ieDf2 is a deficiency of zim-1, zim-2, zim-3, and him-8 generated by MosDel, resulting in single-copy insertion of a copy of the C. briggsae unc-119 gene on Chromosome IV. The deletion spans the sequences from the beginning of the zim-1 coding sequence through the ttTi22866 Mos1 insertion site.

Degenerate tail. Posterior half is thin, pale, uncoordinated.

Dauer defective. Notched head. Unlinked double. M-MATING-NO SUCCESS.

Unc-fainter. Stops. Stiff anterior.

Unc-stiff anterior.

Very sluggish as L1, moves better as adult. Weak kinker. Head region stiff. Moves better in reverse, fairly active. Levamisole resistant.

Chemotaxis abnormal. Crawls off plate. Dauer defective. Small. M-MATING-NO SUCCESS.

Chemotaxis defective: Cl-. Eggs laid off bacteria. Small. Recessive. M-MATING+++ 10-30%WT.

3% XO self progeny.

Uncoordinated. May contain a weak daf-2 mutation (sa875). See Ailion and Thomas in Genetics 165: 127-144 2003.

Blebs on tail of adult hermaphrodite. Tail irregular, tail cuticle sometimes separated, tail spike sometimes truncated. Some animals have enlarged excretory canals.

6% XO self-progeny. Males sterile. M-MATING-NO SUCCESS.

Male gonad abnormal. Males sterile. 6% XO self-progeny. M-MATING-NO SUCCESS.

Egg-laying defective. Moderate to severe bloating. 30% make bags of worms. Males mate. [NOTE: Probably contains a tightly linked mutation which cannot be crossed off. Use MT3188 as reference strain. Michael Stern 6\97]

Temperature sensitive dauer constitutive. 100% dauers at 25C. Dauer recovery poor at 15C. Small. M-Mating--no success.

Temperature sensitive dauer constitutive. Maintain at 15C. 100% dauers at 25C. 15% dauer formation at 20C. Long-lived. M-MATING+LOW TEMPERATURE ONLY.

Temperature sensitive dauer constitutive. Crowds. 100% dauers at 25C. Leaky at 20C. Growth slow. M-MATING+LOW TEMPERATURE ONLY.

Dauer defective. Non-crowder. Chemotaxis normal.

Dauer defective. Non-crowder. M-MATING++++ >30%WT.

Dauer defective. Crowds. Leaky. Dauer recovery normal. Chemotaxis defective-Na+. M-MATING++++ >30%WT.

Dauer defective. WT phenotype. M-MATING+++ 10-30%WT.

Amphid defect. Chemotaxis defective (Na+). Crowds. Dauer defective. Very leaky. M-MATING+POOR <1%WT.

Dauer constitutive. Temperature sensitive. Crowds. Chemotaxis normal. Grow at 15C.

Egg-laying deficient. Lineage abnormal. Mechanosensory abnormal. M-MATING++ 1-10%WT. Serotonin def.

20% XO self-progeny at 16C. Recessive. Temperature sensitive. M-MATING++++ >30%WT.

15% XO self-progeny. 6% XXX self-progeny. Low brood size. 78% unhatched eggs. General non-disjunction. Recessive.

3% XO self-progeny. Recessive.M-MATING+++ 10-30%WT.

Recessive. M-MATING++++ >30%WT.

37% XO self-progeny. 6% XXX self-progeny. Recessive. M-MATING++ 1-10%WT. [Background mutation that causes rays 8 and 9 to fuse.]

Temperature sensitive. 2% XO self-progeny at 15C, 12% at 20C, and 27% at 25C. M-MATING++++ >30%WT.

DpyUnc. See also WBPaper0000498 and WBPaper00002306.

Transforms XX to males. Variable expression. Masculinized self-fertile hermaphrodite. tra-2(f70) was crossed into the N2 background to construct a dpy-10 tra-2 unc-4 triple mutant, then crossed to remove the flanking dpy and unc (Jonathan Hodgkin).

Heterozygotes are WT and segregate WT, UncDaf and Lethals. Suppressed Unc in hets. Suppressed Daf in hets. sup-6 is recessive lethal.

Temperature sensitive dauer constitutive. Unc. Closely linked.

Dauer defective. Long.

Dauer defective. Long.

Temperature sensitive dauer constitutive. Long. Unc. Linked closely.

Heterozygotes are wild-type, and segregate wild-type heterozygotes, fertile wild-type males (tra-1 homozygotes), and Dpy. Can recombine: pick individual WT hermaphrodites and check for correct segregation of progeny to maintain.

Heterozygotes are WT and segregate WT, lethal dauers (DAUER-LIKE LARVAE) and Lon. Can recombine; check for correct segregation of progeny to maintain.

Unc. Small. Egg Retainer. Serotonin Def. Recessive. M-MATING-NO SUCCESS.

Balanced well. Animals with the duplication are WT. Animals which have lost the duplication have a hermaphrodite gonad and intestine (they are self-fertile); the rest of the body is male.

Balanced well. Animals with the duplication are WT. Animals which have lost the duplication have a hermaphrodite gonad and intestine (they are self-fertile); the rest of the body is male.

Intragenic revertant of dominant mutation e665. Slight uncoordinated phenotype.

Dominant suppressor of Unc. Dpy. sup-6 is recessive lethal. Heterozygotes are Dpy non-Unc. Pick Dpy non-Unc to maintain.

Dominant suppressor of Unc. Movement almost WT.

Caenorhabditis elegans wild isolate. Apparently identical to N2. Isolated by Carl Johnson in June 1973 in a garden in Altadena, CA. Sample obtained from laboratory of R.E. Russell in October 1980. Sent to CGC in Jan. 1997. Reference WBG 10(2) 140-141. For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Delayed hatching from egg shell. QL and descendant cells migrate forward instead of backward (incomplete penetrance).

Selfed hermaphrodites produce 5% males and 2% XXX hermaphrodites (percent of viable progeny), and 30% unhatched eggs (percent of total progeny).