Search Strains

Long. Can split in two.

Hyperactive. n1134 is a G to A substitution in the initiation codon of goa-1.

Cells that normally die survive. [3/02: A mutation that was not reported (nucleotide 1251 C-> T causing codon 80 ->ochre) was found by Tak Hung. It turns out the mutation was misannotated in the original paper (Development, 1992, 116:309). Bob Horvitz also confirmed the discovery.

Unc. Cells that normally die survive.

Dpy. Cells that normally die survive.

n1165 blocks cell death. Temperature sensitive Egl. Semi-dominant Egl.

Non-Egl.

WT. Intragenic revertant.

WT revertant.

WT revertant.

WT revertant.

WT revertant.

WT revertant.

WT revertant.

Unc. Egl.

Temperature sensitive Multi-vulva. Slow growing. Unc-coils, jerky going backward. Small.

n484: Egl, displaced muscles. e2225: Unc-lethargic and kinker.

Pick DpyUnc worms to maintain, as e1913 is a dominant Unc and a recessive lethal. Actually, the Uncs are not very Dpy, so pick non-Dpy-looking animals that don't move well. e1913 previously called unc-110.

Temperature sensitive. About 50% HSN missing. Mab confirmed by Hillel Schwartz 12/5/00. Rec'd new stock from Horvitz lab 12/2000. [NOTE: The genotype of this strain was incorrectly described as carrying mab-5(mu114); however, the actual allele is mab-5(mu14).]

DpyUnc. Segregates males.

Lon. Unc-ts.

Vulvaless.

Dpy.

Maintain by picking wild-type animals raised at 25C. Heterozygotes will be wild-type and segregate wild-type, Unc, and Sterile Muv. The phenotype of homozygous lin-13 hermaphrodites segregating from a heterozygous mother depends on the temperature at which the strain was grown. At 25C, homozygous hermaphrodites segregating from a heterozygote are both Muv and sterile. At 20C, ~1/2 of hermaphrodites segregating from a heterozygote are sterile, but only a few are Muv. At 15C, hermaphrodites segregating from a heterozygote are almost wild type in appearance and fertility. However, if the progeny of these 15C animals are grown at 15C, all are sterile and some are Muv. If the progeny of these 15C animals are grown at 25C, then some animals arrest during larval growth and the rest are both sterile and Muv. Reference: Ferguson EL & Horvitz HR. Genetics. 1985 May;110(1):17-72. PMID: 3996896.

Cat. Serotonin minus.

Kinker Unc, neuronal lineage defects including lineage reiterations and abnormal corpse pattern in ventral cord. Allele causes W113 opal.

Synthetic Muv. n433 is lin-15 Class A allele.

bwIs4 [fax-1::GFP + rol-6(su1006)]. Rollers. GFP reporter with expression in various neurons and DTC.

Quinine avoidance defective. In qui-1(gb404) a CAA to TAA transition at position 11215 of Y45F10B generates a stop codon in the fifth exon of Y45F10B.10. Putative null allele.

Heterozygotes are WT and GFP+. mIn1 homozygotes are Dpy and GFP+. dna-2 homozygotes are non-GFP.  Reference: Lee, K.H., Lee, M.H., Lee, T.H., Han, J.W., Park, Y.J., Ahnn, J., Seo, Y.S. and Koo, H.S. (2003). Mol Cell 15, 81-6.

Reduced apoptosis after gamma-ray treatment compared to N2. Double mutant exhibits hypersensitivity to gamma rays similar to brc-1(tm1145) alone. Reference: Ryu JS, et al; PLoS One. (In Press).

wdEx455 [acr-14::GFP + unc-119(+)]. Pick non-Unc to maintain.

wdEx457 [F39B2.8::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in pharyngeal muscles, posterior ventral cord motor neurons, tail neurons, and a few head neurons. Construct made by M. Vidal lab; candidate unc-37 target gene. F39B2.8 also known as gpr-158.

wdEx575 [ZC155.2::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expressed in cholinergic motor neurons, head & tail , neurons, and excretory cell. Construct made by Marc Vidal's group at Harvard as part of the promoterome project.

wdEx595 [F08G12.1::GFP + unc-119(+)]. Pick non-Unc to maintain. Construct made by Marc Vidal's group at Harvard as part of the promoterome project.

wdIs62 contains [ceh-12::GFP + unc-119(+)]. Superficially wild-type.

wdIs51 [F49H12.4::GFP + unc-119(+)]; likely integrated in X. GFP expression in PVD.

wdIs52 [F49H12.4::GFP + unc-119(+)].

wdIs52 [F49H12.4::GFP + unc-119(+)]. PVD defects in primary branch guidance, number of secondary branches, and tertiary branches are longer than wild-type.

wdEx848 [mec-4::mCherry + unc-119(+)]. Pick non-Unc to maintain. mCherry expression in all six touch neurons. Array generated by bombardment; ~90% transmission rate. Reference: Smith CJ, O'Brien T, et al. Neuron. 2013 July 24; 79.

otIs458[ceh-63p::GFP + unc-119(+)]. GFP expression in DVC neurons. Can be used to isolate DVC by FACS. Derived by outcrossing parental strain OH11974 to remove him-5(e1490). Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).

wdIs132 [gcy-35::GFP]. uIs152 [mec-3::RFP]. AVM neurons are labeled with GFP and RFP. Can be used to isolate AVM by FACS. wdIs132 arose from spontaneous integration of kyEx1162 [gcy-35::GFP]. Used by CeNGEN project for RNA-Seq (https://www.cengen.org/).

wdEx257 [unc-4::3XFLAG::pab-1 + (pSV17) unc-119 minigene]. Animals exhibit slight forward movement defect. Good antibody staining witih anit-FLAG M2 antibody (Sigma). 100% penetrant array. Expressed in all unc-4 neurons. Ectopically expressed in at least one head neuron (dorsal to pharynx, between two bulbs).

wdEx290 [acr-15::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in VNC, head neurons, and tail neurons. Construct made by M. Vidal lab; candidate unc-37 target gene.

wdEx329 [F09C3.2::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in ventral nerve cord. Construct made by M. Vidal lab; candidate unc-37 target gene.

wdEx345 [F55C12.4::GFP + unc-119(+)]. Pick non-Unc to maintain. GFP expression in VNC, head neurons, and tail neurons. Construct made by M. Vidal lab; candidate unc-37 target gene.