Caenorhabditis Genetics Center (CGC)

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Strain Species Genotype
MT1522 C. elegans ced-3(n717) IV. Show Description
Abnormal cell death. Cells that normally die survive.
MT1743 C. elegans ced-3(n718) IV. Show Description
n718 is a strong allele of ced-3.
MT3002 C. elegans ced-3(n1286) IV. Show Description
Absence of cell death. Strong allele.
MT5166 C. elegans ced-3(n1040) IV. Show Description
MT5353 C. elegans ced-3(n1949) IV. Show Description
Strong allele of ced-9. Reference: (1999) Genetics 153(4):1655-71.
MT6347 C. elegans ced-3(n2433) IV. Show Description
n2433 is a suppressor of ced-9(n1950n2161).
MT7632 C. elegans ced-3(n2432) IV. Show Description
Suppressor of ced-9(n1950 n2162). Reference: (1999) Genetics 153(4):1655-71.
MT8309 C. elegans ced-3(n2921) IV. Show Description
Suppressor of ced-9(n1950n2161) maternal effect lethality.
MT8312 C. elegans ced-3(n2877) IV. Show Description
Medium strong allele. Suppressor of ced-9(n1950n2161) maternal effect lethality.
MT8313 C. elegans ced-3(n2885) IV. Show Description
Medium-strong alllele; suppresses of ced-9(n1950 n2162) maternal effect lethality. Reference: (1999) Genetics 153(4):1655-71.
MT8319 C. elegans ced-3(n2888) IV. Show Description
Suppressor of ced-9(n1950n2161) maternal effect lethality. Strong allele of ced-3.
MT8347 C. elegans ced-3(n2452) IV. Show Description
MT8352 C. elegans ced-3(n2439) IV. Show Description
Suppressor of ced-9(n1950n2161) maternal effect lethality.
MT8354 C. elegans ced-3(n2454) IV. Show Description
n2454 is a strong allele of ced-3.
MT8696 C. elegans ced-3(n2449) IV. Show Description
n2449 is a weak allele of ced-3.
MT8699 C. elegans ced-3(n2424) IV. Show Description
n2424 is a weak allele of ced-3.
RB2071 C. elegans ced-3(ok2734) IV. Show Description
C48D1.2. Homozygous. Outer Left Sequence: CAAAAGGACGCTCTGCCTAT. Outer Right Sequence: TGTCGTGTCGAGACCAGGTA. Inner Left Sequence: AGCAGATCGATTGTTGTTCAAG. Inner Right Sequence: TTGGTCCCAAAAACCAAAAA. Inner Primer PCR Length: 1118 bp. Deletion Size: 682 bp. Deletion left flank: GGCTTTCGCTCCAAAGAATATAAAATAATC. Deletion right flank: TTAAATATCAGTTTACATTAGGAACAAGGC. Insertion Sequence: TT. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
DL308 C. elegans ced-3(n717) IV; mxIs28 X. Show Description
mxIs28 [ceh-20p::ceh-20::YFP + lin-15(+)] X. Reference: Potts MB, Wang DP, & Cameron S. Dev Biol. 2009 May 15;329(2):374-85.
KX84 C. elegans ced-3(n2452) IV; bcIs39 V. Show Description
bcIs39 [lim-7p::ced-1::GFP and lin-15(+)] V.  Resistant to germ cell apoptosis. No apoptosing germ cells are decorated by CED-1::GFP in the gonad. ced-3 deletion confirmed by genomic triple primer PCR with 5’-AGTTCACCGTGACAGCGTCTCTTC-3’, 5’-CGATTACGACTTGAACTGTATCCGA-3’, and 5’-TCTTGTGTAAACGAGATTTGCAATG-3’. Homozygous ced-3(n2452) yields only 1,110 bp product. Outcrossed heterozygotes yield both 1,411 bp (wild type ced-3) and 1,110 bp products. [NOTE: (11-05-2019) A user has reported this strain exhibits temperature-sensitive sterility when raised at 25C.]
OH4322 C. elegans ced-3(ot289) IV; vtIs1 V. Show Description
vtIs1 [dat-1p::GFP + rol-6(su1006)] V. Rollers. The undead sisters of CEPvs and PVDs express dat-1::GFP.
MT2405 C. elegans ced-3(n717) unc-26(e205) IV. Show Description
Unc. Abnormal cell death. Cells that normally die survive.
MT2563 C. elegans ced-3(n1165) IV; egl-1(n487) V. Show Description
n1165 blocks cell death. Temperature sensitive Egl. Semi-dominant Egl.
MT5811 C. elegans ced-1(e1735) I; ced-3(n717) IV. Show Description
MT7386 C. elegans ced-9(n2812) III; ced-3(n717) IV. Show Description
n2812 is a strong loss-of-function allele and a maternal effect lethal.
MT7735 C. elegans ced-9(n2812) III; ced-3(n2427) IV. Show Description
XR2 C. elegans ced-3(n717) IV; abl-1(ok171) X. Show Description
MT5729 C. elegans dpy-20(e1282) unc-30(e191) ced-3(n717) IV. Show Description
Dpy (ts). Unc. Abnormal cell death. Cells that normally die survive.
MT7169 C. elegans sem-4(n1378) I; ced-5(n2098) ced-3(n2720) IV. Show Description
MT7170 C. elegans sem-4(n1378) I; ced-5(n2098) ced-3(n2719) IV. Show Description
MT7502 C. elegans sem-4(n1378) I; ced-5(n2098) ced-3(n2830) IV. Show Description
XR5 C. elegans lagr-1(gk327) I; hyl-1(ok976) IV; ced-3(n717) IV. Show Description
DA2356 C. elegans ced-1(e1735) I; ced-3(n717) IV; lin-15B&lin-15A(n765) X; adEx2342. Show Description
adEx2342 [efl-3::mCherry::FLAG + LIN-15(+)]. Maintain by picking non-Muv. Fluorescence is dim, but array is stable (>95% transmission).
AY178 C. elegans ynIs78; acEx178. Show Description
ynIs78 [flp-8p::GFP]. acEx178 [flp-8p::ced-3 (p15)::nz + flp-32::cz::ced-3 (p17) + unc-122p::RFP]. AUA interneurons ablated in flp-8p::GFP background. GFP-labelled AUA neurons are missing in the neuronal ablated animals. Pick RFP+ animals to maintain. Reference: Filipowicz A, et al. BMC Biol. 2022 Oct 8;20(1):229. doi: 10.1186/s12915-022-01424-x. PMID: 36209082.
AY179 C. elegans ynIs87; acEx179. Show Description
ynIs78 [flp-8p::GFP]. acEx179 [flp-21p::ced-3 (p15)::nz + ncs-1p::cz::ced-3 (p17) + unc-122p::RFP]. RMG interneurons ablated in flp-21p::GFP background. GFP-labelled RMG neurons are missing in the neuronal ablated animals. Pick RFP+ animals to maintain. Reference: Filipowicz A, et al. BMC Biol. 2022 Oct 8;20(1):229. doi: 10.1186/s12915-022-01424-x. PMID: 36209082.
AY190 C. elegans acEx190. Show Description
acEx190 [tax-2p::CZ::ced-3(p17)::unc-54 3’UTR + lim-6p::ced-3(p15)::NZ::unc-54 3’UTR + myo-3p::mCherry]. Pick mCherry+ to maintain.  ASG is ablated in animals carrying the array by employing a two-component system reconstituted caspase (recCaspase) using the tax-2 and lim-6 promoters.  Strain viable at all temperatures.  Reference Otarigho, B. and Aballay, A., 202. Cell reports, 35(8). PMID: 34038721.
AY191 C elegans acEx191. Show Description
acEx191 [odr-2p::CZ::ced-3(p17)::unc-54 3’UTR+ unc-53p::ced-3(p15)::NZ::unc-54 3’UTR + rol-6(su1006)]. Pick Rollers to maintain.  PVP is ablated in animals carrying the array by employing a two-component system reconstituted caspase (recCaspase) using the odr-2 and unc-53 promoters.  Strain viable at all temperatures.  Reference Otarigho, B. and Aballay, A., 202. Cell reports, 35(8). PMID: 34038721.
CU394 C. elegans smIs10 I. Show Description
smIs10 [ced-3p::ced-3::GFP + rol-6(su1006)] I. Rollers. Reference: Geng X, et al. Nat Struct Mol Biol. 2008 Oct;15(10):1094-101.
KX110 C. elegans ced-9(n1653) mab-5(mu14) III; bcIs39 V. Show Description
bcIs39 [lim-7p::ced-1::GFP and lin-15(+)] V.  Temperature sensitive induction of germ cell apoptosis at 25 C. Large number of germ cells are decorated by CED-1::GFP within 48 h. Activates CED-3-mediated cleavage of IFG-1, visualized by western blot (Contreras, V., et al, 2011).
MT11090 C. elegans mcd-1(n3376) II; nIs106 X. Show Description
nIs106 [lin-11::GFP + lin-15(+)] X. Egl, Him. lin-11::GFP expressed in vulva, head neurons, VC neurons. n3376 enhances ced-3(n2427). Reference: (2007) Genetics 175(4)::1719-33.
MT9371 C. elegans nIs50 IV. Show Description
nIs50 [mec-7::ced-3a + rol-6(su1006)]. Rollers.