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Strain Species Genotype
DQM300 C. elegans egl-43(bmd88[LoxP::gfp::egl-43]) II. Show Description
GFP reporter inserted internally into endogenous egl-43 locus. Reference: Medwig-Kinney TN, et al. Development. 2020 Jan 2;147(1).
DQM494 C. elegans egl-43(bmd136[LoxP::gfp::egl-43(long)]) II. Show Description
GFP reporter inserted into N-terminus of endogenous egl-43 locus specifically tags the long isoform. Reference: Medwig-Kinney TN, et al. Development. 2020 Jan 2;147(1).
RW12181 C. elegans egl-43(st12181[egl-43::TY1::EGFP::3xFLAG]) II. Show Description
egl-43(st12181[egl-43::TY1::EGFP::3xFLAG]) II.
MT2246 C. elegans egl-43(n1079) II. Show Description
Egl. Mate with about 50% of WT efficiency.
PJ1017 C. elegans egl-43(n1079) II; ccIs55 V. Show Description
ccIs55 [unc-54::lacZ + sup-7(st5)] V. Maintain at 25C.
MT3553 C. elegans egl-43(n997) II; unc-76(e911) V. Show Description
n997: Egl, 5HT-S, IMIP-R.
MT2801 C. elegans unc-4(e120) egl-43(n997) II; egl-46(n1127) V. Show Description
Unc. Egl.
MT2816 C. elegans egl-44(n1080) unc-4(e120) egl-43(n997) II. Show Description
Unc. Egl.
DQM1285 C. elegans bmdSi363 I; egl-43(bmd88[egl-43p::egl-43::LoxP::GFP::egl-43]) II. Show Description
bmdSi363 [^SEC^ser-2p::mKate2-STOP-STOP-DAMc1::VHH4GFP] I. Pick Rollers to maintain. Wild-type growth. NanoDam toolkit will allows identification of direct genomic targets of TFs as well as chromatin modifiers. In this system, Dam methylase is fused with a binding reagent, an anti-GFP nanobody (vhhGFP4). Thus, genome-wide profiling can be achieved by combining cell type-specific Dam::vhhGFP4 fusion constructs with GFP knock-in alleles.