More Fields
Strain Species Genotype
CX3709 C. elegans unc-115(ky275) X. Show Description
Unc. Lon.
SP1789 C. elegans unc-115(mn481) X. Show Description
Unc. Isolated from RW7097 and not outcrossed.
BC15831 C. elegans dpy-5(e907) I; sEx15831. Show Description
sEx15831 [rCesF09B9.2a::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525).
MT3790 C. elegans unc-115(e2225) egl-15(n484) X. Show Description
n484: Egl, displaced muscles. e2225: Unc-lethargic and kinker.
SP1981 C. elegans unc-115(mn481) dpy-6(e14) X; stDp2 (X;II)/+. Show Description
Wt strain throws WT and DpyUncs.
VC2739 C. elegans +/szT1 [lon-2(e678)] I; unc-115(ok2640)/szT1 X. Show Description
F09B9.2. Homozygous viable deletion chromosome balanced by lon-2-marked translocation. Heterozygotes are WT, and segregate WT, Lon-2 males, arrested szT1 aneuploids, and ok2640 homozygotes (Unc). Pick WT and check for correct segregation of progeny to maintain. External left primer: TCTCATTTTGGTGACGGTGA. External right primer: AAAGGGCAATGAGTTTGCAC. Internal left primer: AGACGAGATCTGGCATCCAT. Internal right primer: GAGAAGAAGAAAAGGCGCAC. Internal WT amplicon: 1358 bp. Deletion size: 512 bp. Deletion left flank: GCAGAATAAAAATTAAAAAAAAATGTTTAA. Deletion right flank: TTGAATCAGTAGCTGGCTATAGAGCACAAC. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
CH121 C. elegans dgn-1(cg121)/dpy-6(e14) unc-115(mn481) X. Show Description
Heterozygotes are WT and segregate WT, DpyUncs, and Ste (and Gon) cg121 homozygotes.
PB1 C. elegans him-5(e1490) V; unc-115(e2225) vab-3(bx23) X. Show Description
Unc-lethargic and kinker. Throws abnormal males-fused rays 4 and 6.
CX3662 C. elegans lin-15B&lin-15A(n765) X; kyIs121. Show Description
kyIs121 [unc-115::GFP + lin-15(+)]; autosomal. Maintain under normal conditions. Reference: Lundquist E, et al. (1998) Neuron 21(2):385-92.