Search Strains

83% Egl. 4% Sterile.

ku194 is a loss of function allele, likely to be molecular null. Connection of gonad defective, >95% Egl. Anchor cell and uterine seam cell do not fuse. Males can mate. Hermaphrodites are very difficult to mate. Previously called cog-2(ku194).

Point mutation W257-> stop codon. Disrupts P cell nuclear migration at 25C. This leads to an Egl, Unc worm. P cell nuclear migration is normal at 15C. hyp7 nuclear migration is normal at all temperatures.

kuIs29 [egl-13p::GFP + unc-119(+)] V. egl-13 is the new gene name for cog-2. Transcriptional fusion of GFP to egl-13 gene. Nuclear localized. Bright expression in body wall muscles, expressed in uterine pi lineage, extensive neuronal expression. Note that a very low penetrance Cog phenotype is seen in this strain. Transgenes with egl-13 promoter can cause Cog phenotype. Conflicting map data: Wendy Hanna-Rose mapped kuIs129 to the left of dpy-11; Shi lab reported it close to gon-10 and unc-76.

kuIs36 [egl-26::GFP + unc-119(+)] II. Transcriptional fusion of GFP to egl-26 gene. Nuclear localized. Embryonic expression. Expressed in somatic gonad (uterus and spermatheca) and vulva in vulE and vulB in L4. Spermatheca expression persists into adulthood. Expressed in pharyngeal-intestinal junction cells and in tail.

Temperature sensitive Egl.

Semi-dominant suppressor of let-60(n1046). Strong reduction of function mutation. At 20C: <1% Muv, 24% Egl, 6% larval lethal, and SM migration defects.

At 20C: 7% Egl, 22% larval lethal. Vulval lineages WT.

keyIs21 [egl-6p::HisCl::unc-54 3' UTR + egl-6p::mCherry::unc-54 3' UTR + lin-15(+)]. Animals express HisCl and mCherry in the HSN neurons, two head sheath glia, two tail neurons, and 1-2 head neurons. Expression is bright in the head and tail. Animals are slightly egg-laying defective, ~10% of animals are Egl. Reference: Ravi B, et al. J. Neuroscience. 38 (28), 6283-6298.

lb130/+ dominantly suppresses the e15 backing defect and causes recessive sterility. Three phenotypes are present in this strain: backing steriles (lb130 homozygotes), backing fertiles which are bloated (heterozygotes), and non-backing coiled Unc animals which are fertile and bloated (sup-40(+)). Maintain strain by picking fertile animals which can back. egl-1(n487) is included in the strain to facilitate identification of lb130 homozygotes, which grow slowly; their sterility contrasts with the bloating of lb130/+ heterozygotes.

Egl. Unc slightly suppressed; the animals will usually back some. [unc-8(e15); egl-15(n484) animals are extremely Unc and will not back.]

100% Unc. 100% Egl. 15% Lid.

Nuclear Ultrabright GFP::mNeonGreen Fluorescent protein (NuGFP) tag inserted downstream of the endogenous daf-2 gene locus by CRISPR/Cas9 engineering. NuGFP cassette is composed of an intercistronic region (ICR) from the C. elegans SL2-type operon, a SV40 nuclear localization sequence (NLS), the coding sequence of GFP, the coding sequence of mNeonGreen, and egl-13 NLS. The expression of NuGFP is tied to that of the endogenous daf-2, but after trans-splicing, the NuGFP protein is synthesized independently of DAF-2. This high-sensitivity daf-2 expression reporter was readily detectable in most C. elegans cells throughout development and adulthood. Reference: Zhang Y, et al. BioRxiv. 2021 Aug 2. 2007.2031.454567. https://doi.org/10.1101/2021.07.31.454567.

WT at 25C. Muv and Egl at 15C.

Egl.

Egg laying defective. Makes bags of worms. Backward Unc.

Egl. Variably Vul.

Egl. Fails to complement daf-14 as well; small deletion or background?? [NOTE: The CGC has received reports that n476 might be heterozygous in this strain. KP2018 is an outcrossed version of this strain and has been confirmed as homozygous for n476.].

Egl. Odr-see 1998 ECWM #71.

Egg laying defective. Retains late stage eggs. Odr-see 1998 ECWM #71.

Temperature sensitive Egl. Odr-see 1998 ECWM #71.

Egg laying defective. Makes bags of worms. Abnormal vulva.

Egg laying defective. Makes bags of worms. Abnormal vulva. Previously called egl-29.

Egg laying defective. Makes bags of worms. Males mate.

Heterozygotes are WT and segregate WT, Unc-36 (eT1 homozygotes), and dpy-18 lis-1 homozygotes which are Mel, Unc, Egl, and Dpy.

Egg laying defective. Makes bags of worms. Males mate.

Egl-moderate bloating, uncoordinated coiler phenotype. Males do not mate.

Egg-laying defective. Retains late stage eggs. Semi-dominant. Males mate successfully. Egg-laying serotinin sensitive and imipramine resistant.

Egg laying defective. Retains late stage eggs.

Gain of function allele. Small, almost paralyized. Egl. Semi-dominant. daf-C at 27C.

nIs106 [lin-11::GFP + lin-15(+)] X. Egl, Him. lin-11::GFP expressed in vulva, head neurons, VC neurons. n3376 enhances ced-3(n2427). Reference: (2007) Genetics 175(4)::1719-33.

Egg laying defective. Most progeny released, but released late (lima bean or later). Males mate poorly.

Egl.

Egg laying defective. Retains late stage eggs. Temperature sensitive.

Egl.

Egg laying defective. Retains late stage eggs.

Egg laying defective. Retains late stage eggs. Partially temperature sensitive. Semidominant.

Egl. Reference: Genetics (1983) 104:619-47.

Temperature sensitive. pka egl-22.

Egl. Strain contains both a mec mutation and an egl mutation. Touch insensitive. Abnormal vulva. Forms bags of worms.

Egg laying defective. Retains late stage eggs. Slow and Floppy; Long.

Egg laying defective. Retains late stage eggs. partially ts

Egg laying defective. Retains late stage eggs. Temperature sensitive->non or weakly egl at 15C

Egg laying defective. Retains late stage eggs. Partially temperature sensitive.

Semi-dominant Egl.

Egl.

Egg laying defective. Retains late stage eggs. Males mate. Weak kinker. Semidominant.

Egl.

Egg laying defective. Makes bags of worms. Dominant. Sluggish.

Egg laying defective. Retains late stage eggs. Semidominant.