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Strain Species Genotype
MT302 C. elegans lin-12(n302) III. Show Description
Vulvaless. Semi-dominant. Males mate.
MT3063 C. elegans lin-12(n950) III. Show Description
Muv. Semi-dominant.
MT1035 C. elegans lin-12(n137n460) III. Show Description
WT at 25C. Muv and Egl at 15C.
MT1337 C. elegans lin-12(n137) III; nT1 (IV;V). Show Description
MT1341 C. elegans lin-12(n302) III; nT1 (IV;V). Show Description
Vulvaless.
BC13816 C. elegans dpy-5(e907) I; sEx13816. Show Description
sEx13816 contains [rCes R107.8::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525).
JT5205 C. elegans lin-12(n950) III; lag-2(sa37) V. Show Description
lag-2(sa37) is a semi-dominant suppressor of lin-12(n302sd).
JT5210 C. elegans lin-12(n302) III; emb-4(sa44) V. Show Description
emb-4(sa44) is a recessive suppressor of lin-12(n302sd).
JT5218 C. elegans sup-17(sa38) I; lin-12(n302) III. Show Description
sa38 is a weak Egl and a moderate suppressor of lin-12(gf).
JT5222 C. elegans sup-19(sa45) I; lin-12(n302) III. Show Description
sa45 is a weak Egl and a moderate suppressor of lin-12(gf).
JT5277 C. elegans sel-8(sa54) lin-12(n302) III. Show Description
sel-8(sa54) is a recessive suppressor of lin-12(n302sd).
JT5283 C. elegans lin-12(n302) III; sel-7(n1253) X. Show Description
sel-7(n1253) is a recessive suppressor of lin-12(n302sd).
MJ61 C. elegans emb-5(hc61) lin-12(ar170) III. Show Description
ts embryonic lethal. Grows at 15C, 20C. Lethal at 25C. [Also contains a temperature sensitive lin-12 hypomorphic allele called ar170. Has 2 anchor cells. Jane Hubbard, 3/96 See WBPaper00002483. See GS1369 for emb-5 reference strain.]
MT1329 C. elegans lin-12(n302) III; him-5(e1467) V. Show Description
Vulvaless. Segregates males which mate well.
MT2021 C. elegans lin-12(n952) III; him-5(e1467) V. Show Description
Muv. Throws males.
MT2982 C. elegans sel-4(n1259) II; lin-12(n302) III. Show Description
sel-4(n1259) is a recessive suppressor of lin-12(n302sd).
MT3000 C. elegans sel-5(n1254) lin-12(n302) III. Show Description
sel-5(n1254) is a recessive suppressor of lin-12(n302sd). n1254 is cold sensitive.
GS60 C. elegans unc-32(e189) lin-12(n676n930) III. Show Description
Unc. Temperature sensitive hypomorphic lin-12 allele. At restrictive temperature of 25C: 100% Egl in unc-32 background (95% Egl without unc-32); 30% have 2 anchor cells, some proximal mitosis, abnormal vulval lineages but has opening; leaks some eggs; mates with males. At permissive temperature of 15C: 93% Egl(+) and 7% Egl(-) [lin-12(d)-like: no anchor cell and no vulval opening]. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
NOA3 C. elegans lin-41(ma104) I; lin-12(n137n460) III. Show Description
VT757 C. elegans lin-28(n719) I; lin-12(n137n460) III. Show Description
Only gives a decent brood size at 20C. At 15C: Muv/Blip. At 20C: some Muv/Blip, some Blip but not Muv. At 25C: Not Muv, but do have Blip (due to lin-12). Egl- at all temps.
JK1195 C. elegans lin-12(q269) glp-1(q231)/unc-32(e189) III. Show Description
Heterozygotes are WT and segregate WT, Uncs, and Lags (ts). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
MT1965 C. elegans lin-12(n941)/eT1 III; him-5(e1467)/eT1 [him-5(e1467)] V. Show Description
n941 is a lin-12 null allele. e1467 is also carried on eT1.
MT688 C. elegans lin-12(n137)/unc-32(e189) III; him-5(e1467) V. Show Description
Heterozygotes are Muv and segregate Muv, Unc and males. Maintain by picking Muv which throw Uncs.
MT2343 C. elegans dpy-19(e1259) lin-12(n137)/unc-32(e189) lin-12(n137n720) III. Show Description
Heterozygotes are Muv and Egl (n137 is semi-dominant) and segregate DpyMuv and Unc lethals (most arrest as L1-L3, the few survivors are sterile, scrawny, Unc and have a large ventral blip at hte position of the vulva). Maintain by picking non-Dpy non-Unc.
NOA1 C. elegans lin-12(n137n460) III; him-5(e1467) V; let-7(n2853) X. Show Description
NOA4 C. elegans sqt-1(sc13) lin-29(n333) II; lin-12(n137n460) III. Show Description
GS883 C. elegans dpy-5(e61) sel(ar40) I; unc-32(e189) lin-12(n676n930) III. Show Description
DpyUnc. ar40 is a semi-dominant suppressor. At 25C ar40 suppresses the Egl phenotype of ne676n930. At 15C a high percentage of hermaphrodites have a 0 AC-Egl phenotype. ar40 suppresses proximal mitosis. ar40 does not suppress vulval lineage defects. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JK1313 C. elegans lin-12(n941) glp-1(q46)/dpy-19(e1259) unc-69(e587) III. Show Description
Heterozygotes are WT and segregate WT, Dpy(ts) Uncs, and Lag L1 lethals (not ts). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
VC1955 C. elegans lin-12(ok2215) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). Show Description
R107.8. Homozygous sterile deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok2215 homozygotes (sterile with vulval blip). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: AATCTTTTCTCGCAGCTCCA. External right primer: CATACATTTGCGTGTGTCCC. Internal left primer: GGGCTGTCATTCCGTTTCTA. Internal right primer: AAACCTGGGAACACATCGAC. Internal WT amplicon: 3327 bp. Deletion size: 1227 bp. Deletion left flank: ATTAATTCTGTTGGTGTGGTTTGGTTTTAT. Deletion right flank: GATTTCTAGAAAACAAACTGGTTGCTTGAA. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
GS107 C. elegans unc-32(e189) lin-12(n676n930) III; dpy-11(e224) sel-9(ar22) V. Show Description
DpyUnc. At 25C ar22 recessively suppresses the Egl defect of n676n930. At 15C ar22 dominantly suppresses the 2 AC-Egl phenotype of n676n930. Suppresses the vulval lineage defects and proximal mitosis defects of lin-12. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS776 C. elegans unc-32(e189) lin-12(n676n930) III; unc-42(e270) sel-11(ar39) V. Show Description
Unc. At 25C ar39 recessively suppresses the Egl phenotype of n676n930. At 15C a high percentage of hermaphrodites have a 0 AC-Egl phenotype. ar39 recessively suppresses vulval lineage defects and proximal mitosis. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS807 C. elegans unc-32(e189) lin-12(n676n930) III; sqt-3(sc8) sel-1(e1948) V. Show Description
RollerUnc. At 25C e1948 recessively suppresses the Egl phenotype of n676n930. At 15C a high percentage of hermaphrodites have a 0 AC-Egl phenotype. e1948 recessively suppresses vulval lineage defects and proximal mitosis. sc8 previously called rol-4(sc8). See also WBPaper00002448. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS878 C. elegans unc-32(e189) lin-12(n676n930) III; lon-3(e2175) sel-10(ar41) V. Show Description
Long. Unc. At 15C ar41 suppresses the 2 AC (anchor cell) phenotype of n676n930. (Strain GS878 does not contain sel(arX) and therefore does not suppress the Egl phenotype at 25C.) See also WBPaper00002966. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
MT2351 C. elegans lin-10(e1439) I; dpy-19(e1259) lin-12(n137)/unc-32(e189) lin-12(n137n720) III. Show Description
Heterozygotes are Muv. Segregates DpyMuv and Unc.
NOA2 C. elegans lin-12(n137n460) III; him-5(e1467) V; unc-3(e151) let-7(mn112) X. Show Description
PS3996 C. elegans lin-10(e1439) I; dpy-19(e1259) lin-12(n137)/unc-32(e189) lin-12(n137n720) III; syIs57. Show Description
syIs57 [chd-3::CFP]. Anchor cell present in approx. 1% d/o animals.
GS1369 C. elegans emb-5(hc61) III. Show Description
Temperature sensitive maternal effect lethal if shifted from 15C to 25C at the L4 and later stages; sterile if it gets past embryogenesis before being shifted. Maintain at 15C. MJ61 contains a lin-12 allele (ar170) which results in 2 anchor cells. GS1369 was derived by outcrossing MJ61 to remove ar170. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Added 10/2005: Zhuoyu Ni in Sylvia Lee's lab did not find the published missense mutation in this strain. It does have the ts sterile/lethal phenotype still.
GS2381 C. elegans sel-5(ok149) III. Show Description
WT. Suppresses the egg-laying defect of lin-12(n302). Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS3012 C. elegans spr-1(ar200) V. Show Description
ar200 does not display any apparent phenotype. It suppresses the Egl defect of both sel-12(ar171) and sel-12(ar131), and displays genetic interactions with various lin-12 and glp-1 alleles. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS4249 C. elegans unc-4(e120) II; arIs82. Show Description
arIs82 [lin-12::GFP + egl-17p::lacZ + unc-4(+)]. Reference: Shaye DD, Greenwald I. Nature. 2002 Dec 12;420(6916):686-90. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
GS4335 C. elegans arIs41 II. Show Description
arIs41 [lin-12::GFP + rol-6(su1006)]. Rollers. Reference: Levitan D, Greenwald I. Development. 1998 Aug;125(16):3101-9. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
JK590 C. elegans glp-1(q35)/eT1 III; him-5(e1490)/eT1 V. Show Description
Heterozygotes are wild-type and segregate wild-type heterozygotes, glp-1(q35) homozygotes (Muv steriles), eT1 homozygotes (Unc-36), and males. glp-1(q35) has a semi-dominant multi-vulva phenotype as well as the loss-of-function Glp phenotype (sterility and embryonic lethality). The q35 mutation is a nonsense mutation that eliminates 122 C-terminal amino acids including a PEST sequence. The C terminus was thought to contain a negative regulatory domain that inactivates glp-1 in the VPCs; the inappropriate glp-1(q35) activity can substitute for lin-12 vulval fate determination. References: Austin J & Kimble J. Cell. 1987 Nov 20;51(4):589-99. Mango S, et al. Nature. 1991 Aug 29;352(6338):811-5.
JT37 C. elegans lag-2(sa37) V. Show Description
Semidominant suppressor of lin-12(d) alleles.
MT2769 C. elegans sup-17(n1258) I. Show Description
sup-17(n1258) is a recessive suppressor of lin-12(n302sd).
MT2941 C. elegans sup-17(n1305) unc-29(e1072) I. Show Description
ts. Dpy. Egl. sup of lin-12(d).
MT2966 C. elegans sup-17(n1313) unc-29(e1072) I. Show Description
Suppressor of lin-12(d).
MT2967 C. elegans sup-17(n1314) unc-29(e1072) I. Show Description
Suppressor of lin-12(d).
MT2970 C. elegans sup-17(n1317) unc-29(e1072) I. Show Description
Suppressor of lin-12(d).
OP72 C. elegans unc-119(ed3) III; wgIs72. Show Description
wgIs72 [lin-12::TY1::EGFP::3xFLAG(92C12) + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov M, et al. Cell. 2012 Aug 17;150(4):855-66. Strain was constructed as part of the Regulatory Element Project, part of modENCODE (http://www.modencode.org)