Search Strains

meIs8 [pie-1p::GFP::cosa-1 + unc-119(+)] II. ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. Single copy transgene inserted into chromosome IV (cxTi10882) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in germ line and early embryos. This strain can be used for auxin-inducible degradation (AID) in germ line and early embryos. References: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Zhang et al., Elife. 2018 Mar 9;7. pii: e30789.

meIs8 [pie-1p::GFP::cosa-1 + unc-119(+)] II. ieSi38 [sun-1p::TIR1::mRuby::sun-1 3'UTR + Cbr-unc-119(+)] IV. Single copy transgene inserted into chromosome IV (cxTi10882) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in germ line and early embryos. This strain can be used for auxin-inducible degradation (AID) in germ line and early embryos. References: Zhang L, et al. Development. 2015 Nov 9. pii: dev.129635. Zhang et al., Elife. 2018 Mar 9;7. pii: e30789.

40% XO self progeny. Recessive.

37% XO self progeny. Recessive.

Weak Him. Produces unhatched eggs.

Weak Him. Produces unhatched eggs.

Unc. Weak Him. Produces unhatched eggs.

mIs11 [myo-2p::GFP + pes-10p::GFP + F22B7.9::GFP]. Pick WT with dim GFP+ in pharynx to maintain. mIs11 homozygotes will quickly overtake the population if not selected against. GFP expression in 4-cell embryos, pharyngeal muscle and gut. Heterozygotes are WT with dim GFP signal in the pharynx. mIs11 homozygotes are WT with bright GFP in the pharynx. rad-51(lg8701) homozygotes are non-GFP and throw dead eggs.

Heterozygotes are Unc and segregate Uncs, dead eggs, and Pvul slow growing tm2610 homozygotes. Pick Unc to maintain and check for correct segregation of progeny. Reference: Bhalla N, et al. PLoS Genet. 2008 4(10) e1000235.

ieSi1 [htp-3p::GFP::him-8 + unc-119(+)] II. ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. ieSi1 is prone to silencing; GFP might not be visible at lower magnifications. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Wynne DJ, et al. J Cell Biol. 2012 Jan 9;196(1):47-64.

mIs11 [myo-2p::GFP + pes-10p::GFP + F22B7.9::GFP]. Heterozygotes are wild-type with dim GFP signal in the pharynx. mIs11 homozygotes are wild-type with bright GFP in the pharynx. ieDf2 homozygotes (non-GFP) develop normally but produce 97.5% inviable embryos and a high frequency of males among the surviving self-progeny. Pick WT with dim GFP+ in pharynx to maintain. mIs11 homozygotes will quickly overtake the population if not selected against. GFP expression in 4-cell embryos, pharyngeal muscle and gut. ieDf2 is a deficiency of zim-1, zim-2, zim-3, and him-8 generated by MosDel, resulting in single-copy insertion of a copy of the C. briggsae unc-119 gene on Chromosome IV. The deletion spans the sequences from the beginning of the zim-1 coding sequence through the ttTi22866 Mos1 insertion site.

Multi-vulva (Muv).

Unc. DNAse undetectable. Gut DNA fluoresence abnormal. M-MATING-NO SUCCESS.

Mild Twitcher Unc.

Dauer defective. Notched head. Unlinked double. M-MATING-NO SUCCESS.

Unc. Chemotaxis abnormal. Short. Variable Dpyish. M-MATING++ 1-10%WT.

Chemotaxis abnormal. Rounded head.

Chemotaxis defective: Cl-. Dauer recovery slow. Movement almost wild-type. Recessive. M-MATING+++ 10-30%WT.

Unc, coiler. Lannate sensitive.

Segregates males (5%).

3% XO self progeny.

Dpy. M-MATING+++ 10-30%WT. Weakly semi-dominant.

Unc.

Twitcher Unc.

Unc-almost paralyzed. Growth slow. Not revertible DES.

Unc-severe kinker. Small. Scrawny. Flaccid. Little movement. Slow pharyngeal pumping.

Coiler Unc. Growth slow.

Dpy.

Slightly slow Unc. Movement almost wild-type. Body muscle abnormal. M-MATING++ 1-10%WT.

Blebs on tail of adult hermaphrodite. Tail irregular, tail cuticle sometimes separated, tail spike sometimes truncated. Some animals have enlarged excretory canals.

Throws 11% males.

Paralyzed Unc. Small.

Paralyzed Unc.

Temperature sensitive. Lineage abnormal. Multi-vulva. M-MATING+POOR <1%WT.

Dpy. Temperature sensitive. Lethal cold sensitive. Male lethal. M-MATING++ 1-10%WT.

Slow but normal development, disorganized muscle structure, somewhat Egl.

Mechanosensory abnormal. Neurons/Touch abnormal. Lethargic. M-MATING++ 1-10%WT.

Dauer defective. Non-crowder. Chemotaxis normal.

Weak kinker.

Amphid defect. Chemotaxis defective (Na+). Crowds. Dauer defective. Very leaky. M-MATING+POOR <1%WT.

Vulvaless. Recessive. M-MATING+++ 10-30%WT.

15% XO self-progeny. 6% XXX self-progeny. Low brood size. 78% unhatched eggs. General non-disjunction. Recessive.

37% XO self-progeny. 6% XXX self-progeny. Recessive. M-MATING++ 1-10%WT. [Background mutation that causes rays 8 and 9 to fuse.]

Unc.

Unc.

Slow moving Unc. Recessive. M-MATING+POOR <1%WT.

Heterozygotes are WT and segregate WT, UncDaf and Lethals. Suppressed Unc in hets. Suppressed Daf in hets. sup-6 is recessive lethal.

Semi-dominant Dpy. Mapping marker standard. M-MATING++ 1-10%WT.

DpyUnc. e184 is semi-dominant.

Paralyzed Unc. Recessive. M-MATING-NO SUCCESS.