Laboratory Information
| Name | NK View on WormBase |
|---|---|
| Allele designation | qy |
| Head | David Royal Sherwood |
| Institution | Duke University |
| Address | 124 Science Dr. FFSC4244 PO BOX 90338 Durham 27708-0338 United States |
| Website | http://www.biology.duke.edu/sherwoodlab/Welcome.html |
| Gene classes |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| DQM1152 | bmdSi243 I; ljf3(unc-34::mNG[C1]^3xFlag::AID) V; qy41(lam-2::mKate2) X. | C. elegans | bmdSi243 (LoxN + cdh-3p::TIR1::F2A::DHB::2xmTurquoise2) I. bmdSi243 is a MosSCI insertion. mNG tag inserted into the C-temrinus of the endogenous unc-34 locus. mKate2 tag inserted into the C-temrinus of the endogenous lam-2 locus. |
| FDU1056 | mig-17(shc19[mig-17::mNG +LoxP]) V. | C. elegans | C-terminus of endogenous mig-17 locus tagged with mNeonGreen using CRISPR/Cas9. Reference: Fan J, et al. Elife. 2020 Apr 7;9:e55890. PMID: 32255430 |
| LP511 | lin-3(cp226[lin-3::mNG::3xFLAG]) IV. | C. elegans | mNG and 3xFlag tags inserted into the C-terminus of the endogenous lin-3 locus. |
| NK1339 | rrf-3(pk1426) II; qyIs127 V; qyIs166 X. | C. elegans | qyIs127 [lam-1p::lam-1::mCherry + unc-119(+)] V. qyIs166 [cdh-3p::GFP::CAAX + unc-119(+)] X. Temperature-sensitive sterile; maintain at 20C or lower for optimum fertility. Increased sensitivity to RNAi when compared to wild-type animals. lam-1p::lam-1::mCherry expression can be weak and variable. Reference: Kelley, LC, et al. Developmental Cell. 2019 Feb 11;48(3):313-328.e8. |
| NK1531 | unc-119(ed4) III; qyIs366 V. | C. elegans | qyIs366 [ser-2(prom3)::hpo-30::GFP + unc-119(+)] V. Reporter allows visualization of HPO-30 trans-membrane protein in PVD dendrites. Reference: Zou W, et al. PLoS Genet. 2015 Sep 22;11(9):e1005484. PMID: 26394140. |
| NK1532 | qyIs368 I; unc-119(ed4) III. | C. elegans | qyIs368 [ser-2(prom3)::dma-1::GFP + unc-119(+)] I. Reporter allows visualization of DMA-1 trans-membrane protein in PVD dendrites. Reference: Zou W, et al. PLoS Genet. 2015 Sep 22;11(9):e1005484. PMID: 26394140. |
| NK2009 | dgn-1(qy206[dgn-1::GFP]) X. | C. elegans | GFP tag inserted into the C-terminus of the endogenous dlg-1 locus. |
| NK2115 | cpIs121 I; rrf-3(pk1426) II; rde-1(ne219) V. | C. elegans | cpIs121 [lag-2p::mNG::PH::F2A::rde-1] I. Temperature-sensitive: maintain at 16-20C. RNAi-response variant. RNAi-hypersensitized DTC-specific RNAi strain that labels all rde-1(+) cells with mNeonGreen. Reference: Linden LM, et al. Dev Biol. 2017 Sep 1;429(1):271-284. |
| NK2225 | unc-59(qy50[unc-59::GFP::3xflag::AID]) I. | C. elegans | CRISPR/Cas9-engineered insertion of GFP::3xflag::AID tags into endogenous unc-59/septin locus. Reference: https://www.micropublication.org/journals/biology/micropub-biology-000200/ |
| NK2318 | dgn-1(qy18[dgn-1::mNG]) X. | C. elegans | Superficially wild-type. Reference: Jayadev R, et al. J Cell Biol. 2019 Aug 6. pii: jcb.201903124. doi: 10.1083/jcb.201903124. |
| NK2322 | cle-1(qy22[cle-1::mNG+loxP]) I. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2324 | ina-1(qy23[ina-1::mNG]) III. | C. elegans | Superficially wild-type. Reference: Jayadev R, et al. J Cell Biol. 2019 Aug 6. pii: jcb.201903124. doi: 10.1083/jcb.201903124. |
| NK2326 | emb-9(qy24[emb-9::mNG+loxP]) III. | C. elegans | Superficially wild-type. Slow growth. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2335 | lam-2(qy20[lam-2::mNG+LoxP]) X. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. Reference: Jayadev R, et al. J Cell Biol. 2019 Aug 6. pii: jcb.201903124. doi: 10.1083/jcb.201903124. |
| NK2353 | agr-1 (qy27[agr-1::mNG+loxP]) II. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2404 | epi-1(qy31[epi-1::mNG+loxP]) IV. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2413 | sdn-1(qy29[sdn-1::mNG+loxP]) X. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2422 | him-4(qy33[him-4::mNG+loxP]) X. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2425 | lam-3(qy28[lam-3::mNG+loxP]) I. | C. elegans | Superficially wild-type. Slow growth. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2436 | pat-3(qy36[pat-3::mNG]) III. | C. elegans | Superficially wild-type. Reference: Jayadev R, et al. J Cell Biol. 2019 Aug 6. pii: jcb.201903124. doi: 10.1083/jcb.201903124. |
| NK2442 | mig-6(qy37[mNG+loxP::mig-6]) V. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. mNeonGreen is inserted at the N-terminus right before a putative proprotein convertase cleavage site and Western analysis indicates most of the mNeonGreen is cut from the MIG-1 protein and is diffuse in the extracellular fluid. See Figure S1 in Keeley et al., Dev Cell. 2020 Jul 6;54(1):60-74.e7. doi: 10.1016/j.devcel.2020.05.022. PMID: 32585132 |
| NK2443 | nid-1(qy38[nid-1::mNG+loxP]) V. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2444 | pxn-2(qy39[pxn-2::mNG+loxP]) X. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2445 | pxn-1(qy40[pxn-1::mNG+loxP]) V. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2446 | lam-2(qy41[lam-2::mKate2]) X. | C. elegans | Superficially wild-type. Reference: Jayadev R, et al. J Cell Biol. 2019 Aug 6. pii: jcb.201903124. doi: 10.1083/jcb.201903124. |
| NK2456 | ddr-1(qy43[ddr-1::mNG+loxP]) X. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2457 | ddr-2(qy44[ddr-2::mNG+loxP]) X. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2476 | ina-1(qy46[ina-1::mKate+loxP]) III. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mKate. Insertion site verified by PCR and sequencing. |
| NK2477 | ptp-3(qy47[ptp-3::mNG+loxP]) II. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2478 | deb-1(qy48[deb-1::mNG + LoxP]) IV. | C. elegans | CRISPR/Cas9 insertion of mNeonGreen tag into endogneous deb-1 locus. Low penetrance Rup and Pvl. Reference: Park K, et al. eLife. 2023 Jul 5;12:RP87037. doi: 10.7554/eLife.87037. PMID: 37405383. |
| NK2479 | pat-2(qy49[pat-2::2xmNG]) III. | C. elegans | Superficially wild-type. Reference: Jayadev R, et al. J Cell Biol. 2019 Aug 6. pii: jcb.201903124. doi: 10.1083/jcb.201903124. |
| NK248 | unc-119(ed4) III; qyIs10 IV. | C. elegans | qyIs10 [lam-1p::lam-1::GFP + unc-119(+)]. Reference: Ziel JW, et al. Nat Cell Biol. 2009 Feb;11(2):183-9. |
| NK2500 | unc-52(qy53[unc-52::mNG+loxP]) II. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2502 | ten-1(qy56[ten-1::mNG+loxP]) III. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2503 | rap-3(qy57[rap-3::mNG]) IV. | C. elegans | Superficially wild-type. Reference: Jayadev R, et al. J Cell Biol. 2019 Aug 6. pii: jcb.201903124. doi: 10.1083/jcb.201903124. |
| NK2511 | ddr-2(qy64[LoxP]) X. | C. elegans | CRISPR-engineered deletion of ddr-2. Low penetrance Rup. Reference: Park K, et al. eLife. 2023 Jul 5;12:RP87037. doi: 10.7554/eLife.87037. PMID: 37405383. |
| NK2540 | fgt-1(qy65[fgt-1::mNG +loxP]) II. | C. elegans | Superficially wild-type. mNG tag inserted into the endogenous fgt-1 locus. Reference: Garde A, et. al. Dev. Cell. 2022 Mar 28;57(6):732-749.e7. PMID: 35316617 |
| NK2555 | unc-52(qy75[mNG+loxP::unc-52]) II. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2557 | mig-6(qy73[mig-6::mNG+loxP]) V. | C. elegans | Superficially wild-type. Specifically tags the long isoform of mig-6. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2565 | pxn-2(qy76[mNG+loxP::pxn-2]) X. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2579 | fbl-1(qy62[mNG+loxP::fbl-1]) IV. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2580 | spon-1(qy30[spon-1::mNG+loxP]) II. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2581 | gpn-1(qy35[gpn-1::mNG+loxP]) X. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2582 | lon-2(qy55[lon-2::mNG+loxP]) X. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2583 | unc-52(qy80[mNG+loxP (synthetic exon)::unc-52]) II. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2585 | emb-9(qy83[emb-9::mRuby2 + LoxP]) III. | C. elegans | mRuby2G tag inserted into the endogenous emb-9 locus (internal tag). Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218. |
| NK2590 | gon-1(qy45[gon-1::mNG+loxP]) IV. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2604 | emb-9 (qy89[emb-9::mEos2+loxP]) III. | C. elegans | Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. |
| NK2609 | qyIs50 V; qyIs550. | C.elegans | qyIs50 [cdh-3p::moeABD::mCherry + unc-119(+)] V. qyIs550 [zmp-1p::MLS::GFP + unc-119(+)]. Superficially wild-type animals expressing mitochondrial GFP and red F-actin in the anchor cell. Reference: Garde A, et. al. Dev. Cell. 2022 Mar 28;57(6):732-749.e7. PMID: 35316617 |
| NK2617 | qyIs23 II; IqIs80 IV. | C. elegans | qyIs23 [cdh-3p::mCherry::PLCdPH + unc-119(+)] II. lqIs80 [SCMp::GFP::caax] IV. Utse and seam markers. Reference: Gianakas CA, et al. J Cell Biol. 2023 Jan 2;222(1):e202112096. doi: 10.1083/jcb.202112096. PMID: 36282214. |
| NK2621 | eif-1.A(qy90[eif-1.A::mNG]) IV. | C. elegans | mNG tag inserted into the C-terminus of the endogenous eif-1.A locus. |
| NK2629 | fgt-1(tm3165) qyIs23 II; qyIs10 IV. | C. elegans | qyIs23 [cdh-3p::mCherry::PLCdPH + unc-119(+)] II. qyIs10 [lam-1p::lam-1::GFP + unc-119(+)] IV. Reference: Reference: Garde A, et. al. Dev. Cell. 2022 Mar 28;57(6):732-749.e7. PMID: 35316617 |
| NK2631 | qySi99 I; unc-6(ev400) X. | C.elegans | qySi99 [lin-29p::fgt-1::mNG + loxP] I. Unc. Single-copy insertion. unc-6 mutant expressing anchor cell specific fgt-1 glucose transporter. Reference: Garde A, et. al. Dev. Cell 2022 March 21. https://doi.org/10.1016/j.devcel.2022.02.019 |
| NK2632 | qySi99 I. | C. elegans | qySi99 [lin-29p::fgt-1::mNG] I. Single-copy insertion of mNG-tagged fgt-1. Reference: Garde A, et. al. Dev. Cell. 2022 Mar 28;57(6):732-749.e7. PMID: 35316617 |
| NK2633 | elo-1(qy97[elo-1::mNG]) IV. | C. elegans | mNG tag inserted into the C-terminus of the endogenous elo-1 locus. |
| NK2635 | fgt-1(tm3165) II; qyIs50 V. | C. elegans | qyIs50 [cdh-3p::moeABD::mCherry + unc-119(+)] V. fgt-1(tm3165) null mutant expressing an anchor cell specific F-actin marker. Reference: Garde A, et. al. Dev. Cell. 2022 Mar 28;57(6):732-749.e7. PMID: 35316617 |
| NK2637 | unc-119(ed4) III; qyIs553 | C. elegans | qyIs553 [lin-29p::ceGreenGlifon4000 +unc-119(+)]. Superficially wild-type strain expressing green glucose biosensor in the anchor cell. Reference: Garde A, et. al. Dev. Cell. 2022 Mar 28;57(6):732-749.e7. PMID: 35316617 |
| NK2639 | fgt-1(tm3165) II; qyIs50 V; qyIs550. | C.elegans | qyIs50 [cdh-3p::moeABD::mCherry + unc-119(+)] V. qyIs550 [zmp-1p::MLS::GFP + unc-119(+)]. fgt-1 glucose transporter null mutants (tm3165) expressing anchor cell specific mitochondrial matrix localized GFP and F-actin mCherry. Useful for analyzing mitochondrial localization, morphology and dynamics in the absence of glucose import. Reference: Garde A, et. al. Dev. Cell. 2022 Mar 28;57(6):732-749.e7. PMID: 35316617 |
| NK2643 | lin-35(n745) I; unc-52(qy80[mNG+loxP (synthetic exon)::unc-52]) II. | C. elegans | CRISPR/Cas9 insertion of mNeonGreen into the endogenous unc-52 locus in an RNAi-sensitized background. Reference: Gianakas CA, et al. J Cell Biol. 2023 Jan 2;222(1):e202112096. doi: 10.1083/jcb.202112096. PMID: 36282214. |
| NK2644 | lin-35(n745) I; fbl-1(qy62[mNG+loxP::fbl-1]) IV. | C. elegans | CRISPR/Cas9 insertion of mNeonGreen into the endogenous fbl-1 locus in an RNAi-sensitized background. Reference: Gianakas CA, et al. J Cell Biol. 2023 Jan 2;222(1):e202112096. doi: 10.1083/jcb.202112096. PMID: 36282214. |
| NK2645 | lin-35(n745) I; him-4(qy33[him-4::mNG+loxP]) X. | C. elegans | CRISPR/Cas9 insertion of mNeonGreen into the endogenous him-4 locus in an RNAi-sensitized background. Reference: Gianakas CA, et al. J Cell Biol. 2023 Jan 2;222(1):e202112096. doi: 10.1083/jcb.202112096. PMID: 36282214. |
| NK2651 | lin-35(n745) I; emb-9(qy83[emb-9::mRuby2 + LoxP]) III. | C. elegans | CRISPR/Cas9 insertion of mRuby2G into the endogenous emb-9 locus (internal tag) in an RNAi-sensitized background. Reference: Gianakas CA, et al. J Cell Biol. 2023 Jan 2;222(1):e202112096. doi: 10.1083/jcb.202112096. PMID: 36282214. |
| NK2667 | F26E4.7(qy103[F26E4.7::mNG + LoxP]) I. | C. elegans | mNG tag inserted into the endogenous F26E4.7 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218. |
| NK2668 | C16E9.1(qy104[C16E9.1::mNG + LoxP]) X. | C. elegans | mNG tag inserted into the endogenous C16E9.1 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218. |
| NK2669 | F26E4.3(qy105[F26E4.3::mNG + LoxP]) I. | C. elegans | mNG tag inserted into the endogenous F26E4.3 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218. |
| NK2672 | unc-40(qy68[unc-40::mNG + LoxP]) IV. | C. elegans | mNG tag inserted into the endogenous unc-40 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218. |
| NK2673 | unc-5(qy70[unc-5::2xmNG + LoxP]) IV. | C. elegans | 2x mNG tag inserted into the endogenous unc-5 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218. |
| NK2688 | fbn-1(qy107[fbn-1::mNG (internal tag) + LoxP]) III. | C. elegans | mNG tag inserted into the endogenous fbn-1 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218. |
| NK2689 | lin-35(n745) I; qyIs23 II; IqIs80 IV. | C. elegans | qyIs23 [cdh-3p::mCherry::PLCdPH + unc-119(+)] II. lqIs80 [SCMp::GFP::caax] IV. RNAi sensitized strain with utse and seam markers. Reference: Gianakas CA, et al. J Cell Biol. 2023 Jan 2;222(1):e202112096. doi: 10.1083/jcb.202112096. PMID: 36282214. |
| NK2692 | test-1(qy108[test-1::mNG + LoxP]) IV. | C. elegans | mNG tag inserted into the endogenous test-1 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218. |
| NK2694 | bmdSi15 rpl-31(qy110[rpl-31::gfp11]) I. | C. elegans | bmdSi15 [eef-1A.1p::GFP1-10::unc-54 3'UTR + myo-2p::mCherry::tbb-2 3'UTR] I. Split GFP tag (GFP11) inserted into the C-terminus of the endogenous rpl-31 locus. |
| NK2698 | sax-3(qy113[sax-3::mNG + LoxP]) X. | C. elegans | mNG tag inserted into the endogenous sax-3 locus (internal tag). Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218. |
| NK2700 | eva-1(qy114[eva-1::mNG + LoxP]) I. | C. elegans | mNG tag inserted into the endogenous eva-1 locus (internal tag). Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218. |
| NK2701 | nas-39(qy115[nas-39::mNG + LoxP]) X. | C. elegans | mNG tag inserted into the endogenous nas-39 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218. |
| NK2702 | C48E7.6(qy116[C48E7.6::mNG + LoxP]) I. | C. elegans | mNG tag inserted into the endogenous C48E7.6 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218. |
| NK2704 | cpi-2(qy117[cpi-2::mNG + LoxP]) V. | C. elegans | mNG tag inserted into the endogenous cpi-2 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218. |
| NK2705 | col-99(qy118[col-99::mNG (internal tag) + LoxP]) IV. | C. elegans | mNG tag inserted into the endogenous col-99 locus (internal tag). Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218. |
| NK2706 | col-99(qy119[col-99::mNG + LoxP]) IV. | C. elegans | mNG tag inserted into the endogenous col-99 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218. |
| NK2721 | qySi569 I. | C. elegans | qySi569 [cdh-3p::fgt-2::mNG + loxP] I. Superficially wild type animal expressing a single copy insertion of glucose transporter fgt-2 in the anchor cell. Reference: Garde A, et. al. Dev. Cell. 2022 Mar 28;57(6):732-749.e7. PMID: 35316617 |
| NK2728 | cpi-1(qy127[cpi-1::mNG + LoxP]) IV. | C. elegans | mNG tag inserted into the endogenous nas-39 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218. |
| NK2730 | rpl-4(qy128[rpl-4::gfp11]) bmdSi15 I. | C. elegans | bmdSi15 [eef-1A.1p::GFP1-10::unc-54 3'UTR + myo-2p::mCherry::tbb-2 3'UTR] I. Split GFP tag (GFP11) inserted into the C-terminus of the endogenous rpl-41 locus. |
| NK2751 | T19D12.6(qy149[T19D12.6::mNG + LoxP]) II. | C. elegans | mNG tag inserted into the endogenous T19D12.6 locus (internal tag). Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218. |
| NK2764 | adm-4(qy153[adm-4::mNG + LoxP]) X. | C. elegans | mNG tag inserted into the endogenous adm-4 locus (internal tag). Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218. |
| NK2779 | fgt-1(tm3165) II; qyIs553. | C.elegans | qyIs553 [lin-29p::ceGreenGlifon4000 +unc-119(+)]. fgt-1(tm3165) null mutant strain expressing green glucose biosensor in the anchor cell. Reference: Garde A, et. al. Dev. Cell. 2022 Mar 28;57(6):732-749.e7. PMID: 35316617 |
| NK2780 | qySi564 I. | C. elegans | qySi564 [lin-29p::pfk-1.1::mNG +loxP] I. Single-copy insertion. Anchor cell-specific expression of the mNG-tagged glycolytic enzyme PFK-1.1 forms localized puncta at the site of invasion. Reference: Garde A, et. al. Dev. Cell. 2022 Mar 28;57(6):732-749.e7. PMID: 35316617 |
| NK2781 | qySi565 I. | C. elegans | qySi565 [lin-29p::pyk-1a::mNG + loxP] I. Single-copy insertion. Anchor cell-specific expression of the mNG-tagged glycolytic enzyme PYK-1A forms puncta at the site of invasion. Reference: Garde A, et. al. Dev. Cell. 2022 Mar 28;57(6):732-749.e7. PMID: 35316617 |
| NK2782 | qySi566 I. | C.elegans | qySi566 [lin-29p::enol-1a::mNG + loxP] I. Single-copy insertion. Anchor cell specific expression of glycolytic enzyme enol-1a which forms puncta at the invasive side and is also expressed in the nucleus. Reference: Garde A, et. al. Dev. Cell. 2022 Mar 28;57(6):732-749.e7. PMID: 35316617 |
| NK2783 | qySi567 I. | C. elegans | qySi567 [ced-10p::pyk-1a::mNG + loxP] I. Single-copy insertion. Glycolytic enzyme pyk-1a expressed under the ced-10 promoter which shows uterine expression and forms clusters only in the anchor cell. Reference: Garde A, et. al. Dev. Cell. 2022 Mar 28;57(6):732-749.e7. PMID: 35316617 |
| NK2784 | trak-1(qy158[trak-1::mNG + loxP]) I. | C. elegans | trak-1 locus endogenously tagged with mNG at the C-terminus. Reference: Garde A, et. al. Dev. Cell. 2022 Mar 28;57(6):732-749.e7. PMID: 35316617 |
| NK2785 | qySi569 I; fgt-1(tm3165) II. | C.elegans | qySi569 [cdh-3p::fgt-2::mNG + loxP] I. fgt-1 glucose transporter null mutant expressing a single copy insertion of the glucose transporter fgt-2 in the anchor cell. Reference: Garde A, et. al. Dev. Cell. 2022 Mar 28;57(6):732-749.e7. PMID: 35316617 |
| NK2789 | bmdSi15 I; shy61(sec-61.B::GFP11x2) IV. | C. elegans | bmdSi15 [eef-1A.1p::GFP1-10::unc-54 3'UTR + myo-2p::mCherry::tbb-2 3'UTR] I. 2x split GFP tag (GFP11) inserted into the C-terminus of the endogenous sec-61.B locus. |
| NK2790 | qySi121 I. | C. elegans | qySi121 [eef-1A.1p::GFP] I. MosSCI insertion. |
| NK2794 | fgt-1(qy65[fgt-1::mNG + loxP]) II; unc-6(ev400) X. | C.elegans | mNG tag inserted into the endogenous fgt-1 locus. Unc. Reference: Garde A, et. al. Dev. Cell. 2022 Mar 28;57(6):732-749.e7. PMID: 35316617 |
| NK2800 | tct-1(qy161[tct-1::mNG]) I. | C. elegans | mNG tag inserted into the C-terminus of the endogenous tct-1 locus. |
| NK2811 | F25H2.6(qy162[F25H2.6::mNG + LoxP]) I. | C. elegans | mNG tag inserted into the endogenous F25H2.6 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218. |
| NK2827 | snb-1(qy164[snb-1::mNG]) V. | C. elegans | mNG tag inserted into the C-terminus of the endogenous snb-1 locus. |
| NK2902 | bmdSi15 I; rpl-31(qy189[rpl-31::ZF1::GFP11]) I; zif-1(gk117) III; qyIs463. | C. elegans | qyIs463 [lin-29p::zif-1::SL2::mCherry]. bmdSi15 [eef-1A.1p::GFP1-10::unc-54 3'UTR + myo-2p::mCherry::tbb-2 3'UTR] I. ZF1 and split GFP tag (GFP11) inserted into the C-terminus of the endogenous rpl-31 locus. |
| NK2920 | emb-9(qy83[emb-9::mRuby2 + LoxP]) III; gon-1(qy45[gon-1::mNG+loxP]) IV. | C. elegans | CRISPR/Cas9 insertion of mNeonGreen into the endogenous gon-1 locus and mRuby2G tag inserted into the endogenous emb-9 locus (internal tag). Reference: Gianakas CA, et al. J Cell Biol. 2023 Jan 2;222(1):e202112096. doi: 10.1083/jcb.202112096. PMID: 36282214. |
| NK2922 | lin-35(n745) I; gon-1(qy45[gon-1::mNG+loxP]) IV. | C. elegans | CRISPR/Cas9 insertion of mNeonGreen into the endogenous gon-1 locus in an RNAi-sensitized background. Reference: Gianakas CA, et al. J Cell Biol. 2023 Jan 2;222(1):e202112096. doi: 10.1083/jcb.202112096. PMID: 36282214. |
| NK2936 | unc-6(cp190[unc-6::mNG::3xFLAG + LoxP]) X. | C. elegans | mNG and 3xFlag tags inserted into the endogenous unc-6 locus. Superficially wild-type. Reference: Naegeli KM, et al. Dev Cell. 2017 Nov 20;43(4):403-417.e10. doi: 10.1016/j.devcel.2017.10.024. PMID: 29161591 |
| NK2961 | rf-3(pk1426) II; qyIs550. | C. elegans | qyIs550 [zmp-1p::MLS::GFP]. |
| NK2962 | rrf-3(pk1426) II; zmp-1(qy17[zmp-1::mNG::GPI]) III. | C. elegans | mNG and GPI tags inserted into the C-terminus of the endogenous zmp-1 locus. |
| NK2964 | nifk-1(qy126[nifk-1::mNG]) zmp-1(cg115) III. | C. elegans | mNG tag inserted into the C-terminus of the endogenous nifk-1 locus. |
| NK2987 | let-60(qy220[mNG::let-60 + LoxP]) IV. | C. elegans | CRISPR/Cas9 insertion of mNeonGreen tag into endogneous let-60 locus. Fairly high penetrance of L1 rod-like lethality. Reference: Jayadev et al. 2023. Post-embryonic endogenous expression and localization of LET-60/Ras in C. elegans. microPublication Biology. 10.17912/micropub.biology.000931. |
| NK2998 | rrf-3(pk1426) II; qyIs570. | C. elegans | qyIs570 [lin-29p::EMTB::GFP]. |
| NK358 | unc-119(ed4) III; qyIs43. | C. elegans | qyIs43 [pat-3::GFP + ina-1(genomic) + unc-119(+)]. Superficially wild-type. Maintain under normal conditions. Reference: Hagedorn et al., Dev Cell (2009) Aug 17(2):187-98. |
| NK364 | unc-119(ed3) III; qyIs46. | C. elegans | qyIs46 [emb-9p::emb-9::mCherry + unc-119(+)] X. Superficially wild-type with very low penetrance (~5%) rupture. Integrated collagen::mCherry reporter. Reference: Ihara S, et al. Nat Cell Biol. 2011 Jun;13(6):641-51. |
| NK413 | rrf-3(pk1426) II; qyIs50 V. | C. elegans | qyIs50 [cdh-3p::moeABD::mCherry + unc-119(+)] V. |
| NK564 | unc-119(ed4) III; qyEx78. | C. elegans | qyEx78 [Venus::unc-6(deltaSP) + unc-119(+)]. Maintain by picking non-Unc. |
| NK640 | rrf-3(pk1426) II; unc-119(ed4) III; rde-1(ne219) V; qyIs102. | C. elegans | qyIs102 [fos-1ap::rde-1(genomic) + myo-2::YFP + unc-119(+)]. Uterine-specific RNAi. |
| NK651 | unc-119(ed4) III; qyIs108. | C. elegans | qyIs108 [lam-1p::lam-1::Dendra + unc-119(+)]. High expression in basement membranes; cleaner laminin-dendra expression than NK652. Reference: Ihara S, et al. Nat Cell Biol. 2011 Jun;13(6):641-51. |
| NK652 | unc-119(ed4) III; qyIs109. | C. elegans | qyIs109 [lam-1p::lam-1::Dendra + unc-119(+)]. High expression in basement membranes, also accumulates in body wall muscle. Reference: Ihara S, et al. Nat Cell Biol. 2011 Jun;13(6):641-51. |
| NK696 | unc-119(ed4) III; qyIs127. | C. elegans | qyIs127 [lam-1p::lam-1::mCherry + unc-119(+)] V. Reference: Ihara S, et al. Nat Cell Biol. 2011 Jun;13(6):641-51. |
| NK741 | rrf-3(pk1426) II; unc-119(ed4) III; rde-1(ne219) V; qyIs138. | C. elegans | qyIs138 [unc-62p::rde-1(genomic) + myo-2::YFP + unc-119(+)]. VPCs sensitive to RNAi. Vul phenotype from lin-39 RNAi depletion. |
| NK742 | rrf-3(pk1426) II; unc-119(ed4) III; rde-1(ne219) V; qyIs139. | C. elegans | qyIs139 [unc-62p::rde-1(genomic) + myo-2::YFP + unc-119(+)]. VPCs sensitive to RNAi. Vul phenotype from lin-39 RNAi depletion. |
| NK772 | unc-119(ed4) III; qyEx115. | C. elegans | qyEx115 [C11D9.1::GFP + unc-119(+)]. Maintain by picking non-Unc. |
| NK773 | unc-119(ed4) III; qyEx114. | C. elegans | qyEx114 [C28C12.10::GFP + unc-119(+)]. Maintain by picking non-Unc. |
| NK774 | unc-119(ed4) III; qyEx116. | C. elegans | qyEx116 [C14A11.3b::GFP + unc-119(+)]. Maintain by picking non-Unc. |
| NK775 | unc-119(ed4) III; qyEx117. | C. elegans | qyEx117 [C14A11.3a,c::GFP + unc-119(+)]. Maintain by picking non-Unc. |
| NK776 | unc-119(ed4) III; qyEx118. | C. elegans | qyEx118 [exc-5::GFP + unc-119(+)]. Maintain by picking non-Unc. |
| NK777 | unc-119(ed4) III; qyEx121. | C. elegans | qyEx121 [F55C7.7d::GFP + unc-119(+)]. Maintain by picking non-Unc. |
| NK778 | unc-119(ed4) III; qyEx120. | C. elegans | qyEx120 [F55C7.7c::GFP + unc-119(+)]. Maintain by picking non-Unc. |
| NK779 | unc-119(ed4) III; qyEx119. | C. elegans | qyEx119 [F13E6.6::GFP + unc-119(+)]. Maintain by picking non-Unc. |
| NK780 | unc-119(ed4) III; qyEx122. | C. elegans | qyEx122 [F55C7.7e::GFP + unc-119(+)]. Maintain by picking non-Unc. |
| NK781 | unc-119(ed4) III; qyEx123. | C. elegans | qyEx123 [K07D4.7a::GFP + unc-119(+)]. Maintain by picking non-Unc. |
| NK782 | unc-119(ed4) III; qyEx124. | C. elegans | qyEx124 [pix-1::GFP + unc-119(+)]. Maintain by picking non-Unc. |
| NK783 | unc-119(ed4) III; qyEx125. | C. elegans | qyEx125 [R02F2.2::GFP + unc-119(+)]. Maintain by picking non-Unc. |
| NK784 | unc-119(ed4) III; qyEx126. | C. elegans | qyEx126 [T19E10.1::GFP + unc-119(+)]. Maintain by picking non-Unc. |
| NK785 | unc-119(ed4) III; qyEx127. | C. elegans | qyEx127 [ced-5::GFP + unc-119(+)]. Maintain by picking non-Unc. |
| NK786 | unc-119(ed4) III; qyEx128. | C. elegans | qyEx128 [F22G12.5::GFP + unc-119(+)]. Maintain by picking non-Unc. |
| NK860 | unc-119(ed4) III; qyIs161. | C. elegans | qyIs161 [emb-9p::emb-9::Dendra + unc-119(+)]. Reference: Ihara S, et al. Nat Cell Biol. 2011 Jun;13(6):641-51. |
| NK885 | unc-119(ed4) III; qyIs174. | C. elegans | qyIs174 [hlh-2p::GFP::hlh-2 + unc-119(+)]. Translational fusion protein displaying cytoplasmic and nuclear localization; contains 8 kb upstream, N-terminal GFP fusion, full open reading frame and 3'UTR. Reference: Schindler AJ, Sherwood DR. Dev Biol. 2011 Sep 15;357(2):380-91. |
| NK887 | unc-119(ed4) III; qyIs176. | C. elegans | qyIs176 [zmp-1(50-51)p::mCherry::moeABD + unc-119(+)]. Reference: Schindler AJ, Sherwood DR. Dev Biol. 2011 Sep 15;357(2):380-91. |
| PS4865 | unc-119(ed4) III; him-5(e1490) V; syEx684. | C. elegans | syEx684 [unc-119(+) + fos-1a/b::GFP-TL]. Ex line of functional fos-1 gene tagged with GFP at the C-terminus. Pick WT to maintain. Throws males. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. |
| RIE102 | unc-119(ed3) III; ftt-2(tm1486) X; atrIs1. | C. elegans | atrIs1 [ftt-2p::ftt-2::mCherry + unc-119(+)]. Line is slightly sick and burrows. FTT-2::mCherry fusion protein rescues ftt-2(tm1486) deletion mutant. Reference: Linden LM, et al. Dev Biol. 2017 Sep 1;429(1):271-284. PMID: 28648843 |
| SBW292 | ebp-2(wow47[ebp-2::GFP::3xFLAG]) II. | C. elegans | GFP and 3xFlag tags inserted into the C-terminus of the endogenous ebp-2 locus. |
This laboratory hasn't submitted any alleles to the CGC.