Laboratory Information

NameNK View on WormBase
Allele designationqy
HeadDavid Royal Sherwood
InstitutionDuke University
Address 124 Science Dr.
FFSC4244
PO BOX 90338
Durham 27708
United States
Website http://www.biology.duke.edu/sherwoodlab/Welcome.html
Gene classes

Strains contributed by this laboratory

Strain Genotype Species Description
FDU1056 mig-17(shc19[mig-17::mNG +LoxP]) V. C. elegans C-terminus of endogenous mig-17 locus tagged with mNeonGreen using CRISPR/Cas9. Reference: Fan J, et al. Elife. 2020 Apr 7;9:e55890. PMID: 32255430
KG1271 lite-1(ce314) X; ceIs37. C. elegans ceIs37 [myo-3p::lite-1 + myo-3p::GFP]. myo-3 promoter driving expression of lite-1 cDNA allows optogenetic induction of muscle contraction and egg laying. Exposure to blue light induces contraction of the muscles, and egg-laying in adults. Reference: Edwards SL, et al. PLoS Biol. 2008 Aug 5;6(8):e198.
NK1339 rrf-3(pk1426) II; qyIs127 V; qyIs166 X. C. elegans qyIs127 [lam-1p::lam-1::mCherry + unc-119(+)] V. qyIs166 [cdh-3p::GFP::CAAX + unc-119(+)] X. Temperature-sensitive sterile; maintain at 20C or lower for optimum fertility. Increased sensitivity to RNAi when compared to wild-type animals. lam-1p::lam-1::mCherry expression can be weak and variable. Reference: Kelley, LC, et al. Developmental Cell. 2019 Feb 11;48(3):313-328.e8.
NK2115 cpIs121 I; rrf-3(pk1426) II; rde-1(ne219) V. C. elegans cpIs121 [lag-2p::mNG::PH::F2A::rde-1] I. Temperature-sensitive: maintain at 16-20C. RNAi-response variant. RNAi-hypersensitized DTC-specific RNAi strain that labels all rde-1(+) cells with mNeonGreen. Reference: Linden LM, et al. Dev Biol. 2017 Sep 1;429(1):271-284.
NK2225 unc-59(qy50[unc-59::GFP::3xflag::AID]) I. C. elegans CRISPR/Cas9-engineered insertion of GFP::3xflag::AID tags into endogenous unc-59/septin locus. Reference: https://www.micropublication.org/journals/biology/micropub-biology-000200/
NK2318 dgn-1(qy18[dgn-1::mNG]) X. C. elegans Superficially wild-type. Reference: Jayadev R, et al. J Cell Biol. 2019 Aug 6. pii: jcb.201903124. doi: 10.1083/jcb.201903124.
NK2322 cle-1 (qy22[cle-1::mNG+loxP]) I. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2324 ina-1(qy23[ina-1::mNG]) III. C. elegans Superficially wild-type. Reference: Jayadev R, et al. J Cell Biol. 2019 Aug 6. pii: jcb.201903124. doi: 10.1083/jcb.201903124.
NK2326 emb-9 (qy24[emb-9::mNG+loxP]) III. C. elegans Superficially wild-type. Slow growth. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2335 lam-2(qy20[lam-2::mNG+LoxP]) X. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. Reference: Jayadev R, et al. J Cell Biol. 2019 Aug 6. pii: jcb.201903124. doi: 10.1083/jcb.201903124.
NK2353 agr-1 (qy27[agr-1::mNG+loxP]) II. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2404 epi-1(qy31[epi-1::mNG+loxP]) IV. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2413 sdn-1(qy29[sdn-1::mNG+loxP]) X. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2422 him-4(qy33[him-4::mNG+loxP]) X. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2425 lam-3(qy28[lam-3::mNG+loxP]) I. C. elegans Superficially wild-type. Slow growth. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2436 pat-3(qy36[pat-3::mNG]) III. C. elegans Superficially wild-type. Reference: Jayadev R, et al. J Cell Biol. 2019 Aug 6. pii: jcb.201903124. doi: 10.1083/jcb.201903124.
NK2442 mig-6(qy37[mNG+loxP::mig-6]) V. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2443 nid-1(qy38[nid-1::mNG+loxP]) V. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2444 pxn-2(qy39[pxn-2::mNG+loxP]) X. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2445 pxn-1(qy40[pxn-1::mNG+loxP]) V. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2446 lam-2(qy41[lam-2::mKate2]) X. C. elegans Superficially wild-type. Reference: Jayadev R, et al. J Cell Biol. 2019 Aug 6. pii: jcb.201903124. doi: 10.1083/jcb.201903124.
NK2456 ddr-1(qy43[ddr-1::mNG+loxP]) X. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2457 ddr-2(qy44[ddr-2::mNG+loxP]) X. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2476 ina-1(qy46[ina-1::mKate+loxP]) III. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mKate. Insertion site verified by PCR and sequencing.
NK2477 ptp-3(qy47[ptp-3::mNG+loxP]) II. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2479 pat-2(qy49[pat-2::2xmNG]) III. C. elegans Superficially wild-type. Reference: Jayadev R, et al. J Cell Biol. 2019 Aug 6. pii: jcb.201903124. doi: 10.1083/jcb.201903124.
NK248 unc-119(ed4) III; qyIs10 IV. C. elegans qyIs10 [lam-1p::lam-1::GFP + unc-119(+)]. Reference: Ziel JW, et al. Nat Cell Biol. 2009 Feb;11(2):183-9.
NK2500 unc-52(qy53[unc-52::mNG+loxP]) II. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2502 ten-1(qy56[ten-1::mNG+loxP]) III. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2503 rap-3(qy57[rap-3::mNG]) IV. C. elegans Superficially wild-type. Reference: Jayadev R, et al. J Cell Biol. 2019 Aug 6. pii: jcb.201903124. doi: 10.1083/jcb.201903124.
NK2555 unc-52(qy75[mNG+loxP::unc-52]) II. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2556 mig-6(qy72[mig-6::mNG+loxP]) V. C. elegans Reduced brood size but can be maintained as homozygotes. Specifically tags the short isoform of mig-6. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2557 mig-6(qy73[mig-6::mNG+loxP]) V. C. elegans Superficially wild-type. Specifically tags the long isoform of mig-6. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2558 let-2(qy61[Let-2::mNG+loxP]) X. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. 2/28/2021: notified strain received was incorrect - qy61 is homozygous inviable. Anyone wanting this strain should contact the Sherwood lab.
NK2565 pxn-2(qy76[mNG+loxP::pxn-2]) X. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2579 fbl-1(qy62[mNG+loxP::fbl-1]) IV. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2580 spon-1(qy30[spon-1::mNG+loxP]) II. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2581 gpn-1(qy35[gpn-1::mNG+loxP]) X. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2582 lon-2(qy55[lon-2::mNG+loxP]) X. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2583 unc-52(qy80[mNG+loxP (synthetic exon)::unc-52]) II. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2585 emb-9(qy83[emb-9::mRuby2 + LoxP]) III. C. elegans mRuby2G tag inserted into the endogenous emb-9 locus (internal tag). Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218.
NK2590 gon-1(qy45[gon-1::mNG+loxP]) IV. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2604 emb-9 (qy89[emb-9::mEos2+loxP]) III. C. elegans Superficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing.
NK2667 F26E4.7(qy103[F26E4.7::mNG + LoxP]) I. C. elegans mNG tag inserted into the endogenous F26E4.7 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218.
NK2668 C16E9.1(qy104[C16E9.1::mNG + LoxP]) X. C. elegans mNG tag inserted into the endogenous C16E9.1 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218.
NK2669 F26E4.3(qy105[F26E4.3::mNG + LoxP]) I. C. elegans mNG tag inserted into the endogenous F26E4.3 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218.
NK2672 unc-40(qy68[unc-40::mNG + LoxP]) IV. C. elegans mNG tag inserted into the endogenous unc-40 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218.
NK2673 unc-5(qy70[unc-5::2xmNG + LoxP]) IV. C. elegans 2x mNG tag inserted into the endogenous unc-5 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218.
NK2688 fbn-1(qy107[fbn-1::mNG (internal tag) + LoxP]) III. C. elegans mNG tag inserted into the endogenous fbn-1 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218.
NK2692 test-1(qy108[test-1::mNG + LoxP]) IV. C. elegans mNG tag inserted into the endogenous test-1 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218.
NK2698 sax-3(qy113[sax-3::mNG + LoxP]) X. C. elegans mNG tag inserted into the endogenous sax-3 locus (internal tag). Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218.
NK2700 eva-1(qy114[eva-1::mNG + LoxP]) I. C. elegans mNG tag inserted into the endogenous eva-1 locus (internal tag). Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218.
NK2701 nas-39(qy115[nas-39::mNG + LoxP]) X. C. elegans mNG tag inserted into the endogenous nas-39 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218.
NK2702 C48E7.6(qy116[C48E7.6::mNG + LoxP]) I. C. elegans mNG tag inserted into the endogenous C48E7.6 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218.
NK2704 cpi-2(qy117[cpi-2::mNG + LoxP]) V. C. elegans mNG tag inserted into the endogenous cpi-2 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218.
NK2705 col-99(qy118[col-99::mNG (internal tag) + LoxP]) IV. C. elegans mNG tag inserted into the endogenous col-99 locus (internal tag). Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218.
NK2706 col-99(qy119[col-99::mNG + LoxP]) IV. C. elegans mNG tag inserted into the endogenous col-99 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218.
NK2728 cpi-1(qy127[cpi-1::mNG + LoxP]) IV. C. elegans mNG tag inserted into the endogenous nas-39 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218.
NK2751 T19D12.6(qy149[T19D12.6::mNG + LoxP]) II. C. elegans mNG tag inserted into the endogenous T19D12.6 locus (internal tag). Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218.
NK2764 adm-4(qy153[adm-4::mNG + LoxP]) X. C. elegans mNG tag inserted into the endogenous adm-4 locus (internal tag). Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218.
NK2811 F25H2.6(qy162[F25H2.6::mNG + LoxP]) I. C. elegans mNG tag inserted into the endogenous F25H2.6 locus. Reference: Jayadev R, et al. Sci Adv. 2022 May 20;8(20):eabn2265. doi: 10.1126/sciadv.abn2265. PMID: 35584218.
NK358 unc-119(ed4) III; qyIs43. C. elegans qyIs43 [pat-3::GFP + ina-1(genomic) + unc-119(+)]. Superficially wild-type. Maintain under normal conditions. Reference: Hagedorn et al., Dev Cell (2009) Aug 17(2):187-98.
NK364 unc-119(ed3) III; qyIs46. C. elegans qyIs46 [emb-9p::emb-9::mCherry + unc-119(+)] X. Superficially wild-type with very low penetrance (~5%) rupture. Integrated collagen::mCherry reporter. Reference: Ihara S, et al. Nat Cell Biol. 2011 Jun;13(6):641-51.
NK564 unc-119(ed4) III; qyEx78. C. elegans qyEx78 [Venus::unc-6(deltaSP) + unc-119(+)]. Maintain by picking non-Unc.
NK640 rrf-3(pk1426) II; unc-119(ed4) III; rde-1(ne219) V; qyIs102. C. elegans qyIs102 [fos-1ap::rde-1(genomic) + myo-2::YFP + unc-119(+)]. Uterine-specific RNAi.
NK651 unc-119(ed4) III; qyIs108. C. elegans qyIs108 [lam-1p::lam-1::Dendra + unc-119(+)]. High expression in basement membranes; cleaner laminin-dendra expression than NK652. Reference: Ihara S, et al. Nat Cell Biol. 2011 Jun;13(6):641-51.
NK652 unc-119(ed4) III; qyIs109. C. elegans qyIs109 [lam-1p::lam-1::Dendra + unc-119(+)]. High expression in basement membranes, also accumulates in body wall muscle. Reference: Ihara S, et al. Nat Cell Biol. 2011 Jun;13(6):641-51.
NK696 unc-119(ed4) III; qyIs127. C. elegans qyIs127 [lam-1p::lam-1::mCherry + unc-119(+)] V. Reference: Ihara S, et al. Nat Cell Biol. 2011 Jun;13(6):641-51.
NK741 rrf-3(pk1426) II; unc-119(ed4) III; rde-1(ne219) V; qyIs138. C. elegans qyIs138 [unc-62p::rde-1(genomic) + myo-2::YFP + unc-119(+)]. VPCs sensitive to RNAi. Vul phenotype from lin-39 RNAi depletion.
NK742 rrf-3(pk1426) II; unc-119(ed4) III; rde-1(ne219) V; qyIs139. C. elegans qyIs139 [unc-62p::rde-1(genomic) + myo-2::YFP + unc-119(+)]. VPCs sensitive to RNAi. Vul phenotype from lin-39 RNAi depletion.
NK772 unc-119(ed4) III; qyEx115. C. elegans qyEx115 [C11D9.1::GFP + unc-119(+)]. Maintain by picking non-Unc.
NK773 unc-119(ed4) III; qyEx114. C. elegans qyEx114 [C28C12.10::GFP + unc-119(+)]. Maintain by picking non-Unc.
NK774 unc-119(ed4) III; qyEx116. C. elegans qyEx116 [C14A11.3b::GFP + unc-119(+)]. Maintain by picking non-Unc.
NK775 unc-119(ed4) III; qyEx117. C. elegans qyEx117 [C14A11.3a,c::GFP + unc-119(+)]. Maintain by picking non-Unc.
NK776 unc-119(ed4) III; qyEx118. C. elegans qyEx118 [exc-5::GFP + unc-119(+)]. Maintain by picking non-Unc.
NK777 unc-119(ed4) III; qyEx121. C. elegans qyEx121 [F55C7.7d::GFP + unc-119(+)]. Maintain by picking non-Unc.
NK778 unc-119(ed4) III; qyEx120. C. elegans qyEx120 [F55C7.7c::GFP + unc-119(+)]. Maintain by picking non-Unc.
NK779 unc-119(ed4) III; qyEx119. C. elegans qyEx119 [F13E6.6::GFP + unc-119(+)]. Maintain by picking non-Unc.
NK780 unc-119(ed4) III; qyEx122. C. elegans qyEx122 [F55C7.7e::GFP + unc-119(+)]. Maintain by picking non-Unc.
NK781 unc-119(ed4) III; qyEx123. C. elegans qyEx123 [K07D4.7a::GFP + unc-119(+)]. Maintain by picking non-Unc.
NK782 unc-119(ed4) III; qyEx124. C. elegans qyEx124 [pix-1::GFP + unc-119(+)]. Maintain by picking non-Unc.
NK783 unc-119(ed4) III; qyEx125. C. elegans qyEx125 [R02F2.2::GFP + unc-119(+)]. Maintain by picking non-Unc.
NK784 unc-119(ed4) III; qyEx126. C. elegans qyEx126 [T19E10.1::GFP + unc-119(+)]. Maintain by picking non-Unc.
NK785 unc-119(ed4) III; qyEx127. C. elegans qyEx127 [ced-5::GFP + unc-119(+)]. Maintain by picking non-Unc.
NK786 unc-119(ed4) III; qyEx128. C. elegans qyEx128 [F22G12.5::GFP + unc-119(+)]. Maintain by picking non-Unc.
NK860 unc-119(ed4) III; qyIs161. C. elegans qyIs161 [emb-9p::emb-9::Dendra + unc-119(+)]. Reference: Ihara S, et al. Nat Cell Biol. 2011 Jun;13(6):641-51.
NK885 unc-119(ed4) III; qyIs174. C. elegans qyIs174 [hlh-2p::GFP::hlh-2 + unc-119(+)]. Translational fusion protein displaying cytoplasmic and nuclear localization; contains 8 kb upstream, N-terminal GFP fusion, full open reading frame and 3'UTR. Reference: Schindler AJ, Sherwood DR. Dev Biol. 2011 Sep 15;357(2):380-91.
NK887 unc-119(ed4) III; qyIs176. C. elegans qyIs176 [zmp-1(50-51)p::mCherry::moeABD + unc-119(+)]. Reference: Schindler AJ, Sherwood DR. Dev Biol. 2011 Sep 15;357(2):380-91.
PS4865 unc-119(ed4) III; him-5(e1490) V; syEx684. C. elegans syEx684 [unc-119(+) + fos-1a/b::GFP-TL]. Ex line of functional fos-1 gene tagged with GFP at the C-terminus. Pick WT to maintain. Throws males. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
This laboratory hasn't submitted any alleles to the CGC.