Strain Information

Name NK2442   View On Wormbase
Species C. elegans
Genotypemig-6(qy37[mNG+loxP::mig-6]) V.
DescriptionSuperficially wild-type. CRISPR/Cas9 insertion of mNeonGreen. Insertion site verified by PCR and sequencing. mNeonGreen is inserted at the N-terminus right before a putative proprotein convertase cleavage site and Western analysis indicates most of the mNeonGreen is cut from the MIG-1 protein and is diffuse in the extracellular fluid. See Figure S1 in Keeley et al., Dev Cell. 2020 Jul 6;54(1):60-74.e7. doi: 10.1016/j.devcel.2020.05.022. PMID: 32585132
MutagenCrispr/Cas9
Outcrossedx4
Made byQiuyi Chi, Daniel Keeley,Eric Hastie, Ranjay Jayad
Laboratory NK
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