Strain Information

Name NK3304   View On Wormbase
Species C. elegans
GenotypeqySi313 I; unc-119(ed4) III; qyIs629.
DescriptionqySi313 [lin-29p::ucp-4::SL2::mKate2::PLC(delta)PH::3xHA::tbb-2 3'UTR] I. qyIs629 [eef-1A.1p::PercevalHR::unc-54 3'UTR + unc-119(+)]. Ubiquitous somatic expression of ratiometric ATP:ADP biosensor PercevalHR. Anchor cell specific overexpression of mitochondrial uncoupling protein, UCP-4, (expression confirmed by presence of red anchor cell membrane). qySi313 inserted into ttTi4348 mosSCI site (I:-5.32) using CRISPR/Cas9-mediated recombination.
MutagenCrispr/Cas9 & gamma irradiation
Outcrossedx3
Made byIsabel Kenny-Ganzert
Laboratory NK
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