Strain Information

Name NK3237   View On Wormbase
Species C. elegans
Genotypelet-2(qy286[let-2::P2A::PEST::mNG]) X.
DescriptionEndogenous reporter of type IV collagen alpha chain (let-2) translation. mNG tag inserted at the endogenous C-terminus locus with a P2A sequence between the C-terminus of let-2 and mNG. P2A causes let-2 to be translated independently of mNG. Cytosolic mNG is a reporter of translation. Reference: Srinivasan S,et al. J Cell Biol. 2025 224(6):e202412118. doi:10.1083/jcb.202412118 PMID: 40100062.
MutagenCrispr/Cas9
Outcrossedx0
Made bySherwood Lab
Laboratory NK
Reference Srinivasan S, Ramos-Lewis W, Morais MRPT, et al. A collagen IV fluorophore knock-in toolkit reveals trimer diversity in C. elegans basement membranes. J Cell Biol. 2025;224(6):e202412118. doi:10.1083/jcb.202412124
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