Search Strains

nIs267 [myo-2::GFP] integrated in or near eT1. Heterozygotes are wild-type and segregate WT, Dpy Unc, and Unc. Maintain by picking wild-type; check for presence of Unc progeny.

nIs267 [myo-2::GFP] integrated in or near eT1. Heterozygotes are wild-type and segregate WT, Dpy Unc, and Unc. Maintain by picking wild-type; check for presence of Unc progeny.

Pick wild-type GFP+ to maintain. Heterozygotes are wild-type GFP+ and segregate WT green-glowing heterozygotes and non-glowing rba-1 homozygotes. rba-1(n5418) homozygotes are sterile or produce eggs that fail to hatch. qIs48 is an insertion of ccEx9747 (carries myo-2::GFP, pes-10::GFP, and a gut enhancer fused to GFP) onto the hT2 chromosome and is homozygous lethal. Reference: Nakano S, Stillman B, Horvitz HR. Cell. 2011 December 23; 147(7): 1525-1536.

Temperature sensitive. Wild type at 15C. Egl at 25C. HSN-. Egl.

Wild-type revertant of n1324.

Wild type. n1412 loss-of-function suppresses the e1500sd rubberband phenotype.

Wild type. n1415 loss-of-function suppresses the e1500sd rubberband phenotype.

Wild Type.

Maintain by picking wild-type animals raised at 25C. Heterozygotes will be wild-type and segregate wild-type, Unc, and Sterile Muv. The phenotype of homozygous lin-13 hermaphrodites segregating from a heterozygous mother depends on the temperature at which the strain was grown. At 25C, homozygous hermaphrodites segregating from a heterozygote are both Muv and sterile. At 20C, ~1/2 of hermaphrodites segregating from a heterozygote are sterile, but only a few are Muv. At 15C, hermaphrodites segregating from a heterozygote are almost wild type in appearance and fertility. However, if the progeny of these 15C animals are grown at 15C, all are sterile and some are Muv. If the progeny of these 15C animals are grown at 25C, then some animals arrest during larval growth and the rest are both sterile and Muv. Reference: Ferguson EL & Horvitz HR. Genetics. 1985 May;110(1):17-72. PMID: 3996896.

Wild type phenotype. e1500 rubberband phenotype is suppressed by the loss-of-function n234 mutation.

Natural isolate; obtained in April 2002 from compost heap in Lingen, Emsland, North-West Germany; frozen within 5 generations after isolation; microsatellite genotype "EU1". For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Natural isolate; obtained in July 2002 from compost heap in Roxel, Münster, North-West Germany; frozen within 5 generations after isolation; microsatellite genotype "EU3". For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Natural isolate; obtained in July 2002 from compost heap in Roxel, Münster, North-West Germany; frozen within 5 generations after isolation; microsatellite genotype "EU3". For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Natural isolate. Obtained in July 2002 from a compost heap in Roxel, Munster, Northwest Germany. Frozen within 5 generations after isolation. Microsatellite genotype "EU5". For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Natural isolate. Obtained in July 2002 from a compost heap in Roxel, Munster, Northwest Germany. Frozen within 5 generations after isolation. Microsatellite genotype "EU3". For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Natural isolate. Obtained in September 2002 from a compost heap in Mecklenbeck, Munster, Northwest Germany. Frozen within 5 generations after isolation. Microsatellite genotype "EU2". For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Natural isolate. Obtained in September 2002 from a compost heap in Mecklenbeck, Munster, Northwest Germany. Frozen within 5 generations after isolation. Microsatellite genotype "EU2". For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Natural isolate. Obtained in September 2002 from a compost heap in Mecklenbeck, Munster, Northwest Germany. Frozen within 5 generations after isolation. Microsatellite genotype "EU6". For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Natural isolate. Obtained in October 2002 from a compost heap in Roxel, Munster, Northwest Germany. Frozen within 5 generations after isolation. Microsatellite genotype "EU5". For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Natural isolate. Obtained in October 2002 from a compost heap in Roxel, Munster, Northwest Germany. Frozen within 5 generations after isolation. Microsatellite genotype "EU4". For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Natural isolate. Obtained in October 2002 from a compost heap in Roxel, Munster, Northwest Germany. Frozen within 5 generations after isolation. Microsatellite genotype "EU3". For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Natural isolate; obtained in July 2002 from compost heap in Roxel, Münster, North-West Germany; frozen within 5 generations after isolation; microsatellite genotype "EU2". For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Natural isolate; obtained in October 2002 from compost heap in Roxel, Münster, North-West Germany; frozen within 5 generations after isolation; microsatellite genotype "EU3". For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Natural isolate; obtained in October 2002 from compost heap in Roxel, Münster, North-West Germany; frozen within 5 generations after isolation; microsatellite genotype "EU5".

Natural isolate; obtained in October 2002 from compost heap in Roxel, Münster, North-West Germany; frozen within 5 generations after isolation; microsatellite genotype "EU5".

Natural isolate; obtained in October 2002 from compost heap in Roxel, Münster, North-West Germany; frozen within 5 generations after isolation; microsatellite genotype "EU2".

Male-female strain. Isolated from a decaying apple at an apple plantation in Tubingen, Germany.

Natural isolate; obtained in July 2002 from compost heap in Roxel, Münster, North-West Germany; frozen within 5 generations after isolation; microsatellite genotype "EU3".

Male-female strain. Isolated from a decaying apple at an apple plantation in Tubingen, Germany.

Male-female strain. Isolated from a decaying apple at an apple plantation in Tubingen, Germany.

Male-female strain. Isolated from a decaying apple at an apple plantation in Tubingen, Germany.

Natural isolate; obtained in July 2002 from compost heap in Roxel, Münster, North-West Germany; frozen within 5 generations after isolation; microsatellite genotype "EU3".

Natural isolate; obtained in July 2002 from compost heap in Roxel, Münster, North-West Germany; frozen within 5 generations after isolation; microsatellite genotype "EU3".

Natural isolate. Obtained in July 2002 from a compost heap in Roxel, Munster, Northwest Germany. Frozen within 5 generations after isolation. Microsatellite genotype "EU4".

Natural isolate; obtained in July 2002 from compost heap in Roxel, Münster, North-West Germany; frozen within 5 generations after isolation; microsatellite genotype "EU3".

Natural isolate. Obtained in July 2002 from a compost heap in Roxel, Munster, Northwest Germany. Frozen within 5 generations after isolation. Microsatellite genotype "EU3".

Natural isolate; obtained in July 2002 from compost heap in Roxel, Münster, North-West Germany; frozen within 5 generations after isolation; microsatellite genotype "EU3".

C. elegans var Bristol. Generation time is about 3 days. Brood size is about 350. Also CGC reference 257. Isolated from mushroom compost near Bristol, England by L.N. Staniland. Cultured by W.L. Nicholas, identified to genus by Gunther Osche and species by Victor Nigon; subsequently cultured by C.E. Dougherty. Given to Sydney Brenner ca. 1966. Subcultured by Don Riddle in 1973. Caenorhabditis elegans wild isolate. DR subclone of CB original (Tc1 pattern I). [NOTE: This stock might carry a ~1.8 kb deletion in alh-2 in the background. (UPDATE: 03/26/2018 - a user reported the stock they received was homozygous for the alh-2(ot588) mutation.)]

WT C. elegans. From Cambridge collection-originally frozen around 1968: In 1980, in order to establish an ancestral stock, Jonathan Hodgkin thawed one of the earliest frozen tubes of N2, dating from 1968. From this plate J.H. grew up a population en masse (without subculturing) on NGM plates (about 2 generations). Multiple samples of this were frozen in order to provide a reference N2 stock. This set of stock samples was replenished by regrowth in 1985 and 1991, using the same procedure, and a freshly thawed sample was sent to the CGC in 1993. Thus, samples from this frozen stock, called N2 (ancestral), should be only about 6 generations away from the stock used by Sydney Brenner as his standard WT N2. [Isolated from mushroom compost near Bristol, England by L.N. Staniland. Cultured by W.L. Nicholas, identified to genus by Gunther Osche and species by Victor Nigon; subsequently cultured by C.E. Dougherty. Given to Sydney Brenner ca. 1966.] Caenorhabditis elegans wild isolate. Note: N2 (ancestral) has reduced lifespan and fertility relative to the standard CGC N2 strains. See Worm Breeder's Gazette 16(5): 24 (February 1,2001).

C. elegans var Bristol. Self-fertilizing hermaphrodite. Generation time is about 3.5 days at 20C. Male stock maintained by mating. Also CGC reference 257. Isolated from mushroom compost near Bristol, England by L.N. Staniland. Cultured by W.L. Nicholas, identified to genus by Gunther Osche and species by Victor Nigon; subsequently cultured by C.E. Dougherty. Given to Sydney Brenner ca. 1966. Subcultured by Don Riddle in 1973. Caenorhabditis elegans wild isolate. DR subclone of CB original (Tc1 pattern I). [NOTE: (09/07/2018) The Gems Lab has identified a mutation in the gene fln-2 carried in this stock causing an increased lifespan. The effect is quite modest (+11%, median lifespan), but this effect can be more pronounced in other genetic backgrounds.] [NOTE: (03/26/2018) - a user reported the stock they received was homozygous wild-type for alh-2; some N2 stocks carry the ot588 mutation in alh-2.)

5% of the wild type b-glucuronidase activity.

Hypersensitivity to ionizing radiation due to the parg-2(ok980) mutation is rescued by the polq-1(tm2572) mutation.  The double mutant is as sensitive as the wild-type to ionizing radiation. Parental parg-2(ok980) strain outcrossed 6 times; parental polq-1(tm2572) strain outcrossed 5 times. Reference: Bae W, et al. Hypersensitivity to DNA double-strand breaks associated with PARG deficiency is suppressed by exo-1 and polq-1 mutations in Caenorhabditis elegans. The FEBS Journal, In press.

wdIs62 contains [ceh-12::GFP + unc-119(+)]. Superficially wild-type.

wdIs52 [F49H12.4::GFP + unc-119(+)]. PVD defects in primary branch guidance, number of secondary branches, and tertiary branches are longer than wild-type.

Superficially wild-type. wd114 created by the insertion of a tandem array containing seven copies of the GFP11 beta-strand (gfp11x7) in the endogenous oig-1 locus; can be crossed with reporter lines expressing the complementing split GFP fragment (gfp1-10) in specific cell types to facilitate tissue-specific labeling. Reference: He S, et al. Genetics. 2019 Jun;212(2):387-395.

vlc19[ast-1::GFP] II. Superficially wild-type. Endogenous ast-1 locus tagged with GFP using Cas9-triggered homologous recombination. GFP was inserted at the C-terminus using a 9 amino acid flexible linker present in the plasmids (Dickinson et al., 2015). Reference: Lloret-Fernández et al. eLife 2018;7:e32785 DOI: 10.7554/eLife.32785.

vlc28[hlh-3::mNeonGreen] II. Superficially wild-type. Endogenous hlh-3 locus tagged with mNeonGreen using Cas9-triggered homologous recombination. mNeonGreen was inserted at the C-terminus using a 9 amino acid flexible linker present in the plasmids (Dickinson et al., 2015). Reference: Lloret-Fernández et al. eLife 2018;7:e32785 DOI: 10.7554/eLife.32785.

NeonGreen tag inserted into endogenous dat-1 locus by CRISPR/Cas9 engineering. NeonGreen expression in dopaminergic neurons. Superficially wild type. Reference: Jimeno-Martín A, et al. Genome Res. 2022 Mar;32(3):459-473. PMID: 35074859.

Male-Female strain. Do not keep below 20C. Reference (type) isolate for Caenorhabditis sp. 43. Gonochoristic species; isofemale line. Isolated from rotting berries in Chichén Itzá, Yucatán, Mexico (2014).

Caenorhabditis sp. 20 Male-female. Maintain by mating. Isolated by Christian Braendle from rotten Heliconia flowers, Souffriere Forest trail Guadeloupe (16.0328, -61.676) in March 2010.