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RW11970 C. elegans unc-119(tm4063) III; stIs11970. Show Description
stIs11970 [W02D7.6::H1-wCherry + unc-119(+)].
RW11980 C. elegans unc-119(tm4063) III; stIs11980. Show Description
stIs11980 [K05F1.5::H1-wCherry + unc-119(+)].
RW12006 C. elegans unc-119(tm4063) III; stIs12006. Show Description
stIs12006 [ZC376.7a.1::H1-wCherry + unc-119(+)].
RW12009 C. elegans unc-119(tm4063) III; stIs12009. Show Description
stIs12009 [ZK662.4.1::H1-wCherry + unc-119(+)].
RW12012 C. elegans unc-119(tm4063) III; stIs12012. Show Description
stIs12012 [F08C6.7::H1-wCherry + unc-119(+)].
RW12013 C. elegans unc-119(tm4063) III; stIs12013. Show Description
stIs12013 [Y48E1B.7.1::H1-wCherry + unc-119(+)].
RW12022 C. elegans unc-119(tm4063) III; stIs12022. Show Description
stIs12022 [ZK909.4::H1-wCherry + unc-119(+)].
RW12024 C. elegans unc-119(tm4063) III; stIs12024. Show Description
stIs12024 [ZK867.1c::H1-wCherry + unc-119(+)].
RW12061 C. elegans unc-119(tm4063) III; stIs12061. Show Description
stIs12061 [mxl-3::H1-wCherry + unc-119(+)].
RW12063 C. elegans unc-119(tm4063) III; stIs12063. Show Description
stIs12063 [Y5F2A.4::H1-wCherry + unc-119(+)].
RW12065 C. elegans unc-119(tm4063) III; stIs12065. Show Description
stIs12065 [B0414.2::H1-wCherry + unc-119(+)].
RW12164 C. elegans ceh-20(st12164[ceh-20::GFP + loxP + unc-119(+) + loxP] unc-119(tm4063) III. Show Description
ceh-20(st12164[ceh-20::GFP + loxP + unc-119(+) + loxP].
RW12167 C. elegans zfh-2(st12167[zfh-2::GFP + loxP + unc-119(+) + loxP] I; unc-119(tm4063) III. Show Description
zfh-2(st12167[zfh-2::GFP + loxP + unc-119(+) + loxP] I.
RW12170 C. elegans ceh-10(st12170[ceh-10::GFP + loxP + unc-119(+) + loxP]) unc-119(tm4063) III. Show Description
ceh-10(st12170[ceh-10::GFP + loxP + unc-119(+) + loxP]) III.
RW12173 C. elegans egl-44(st12173[egl-44::GFP + loxP + unc-119(+) + loxP]) II; unc-119(tm4063) III. Show Description
egl-44(st12173[egl-44::GFP+loxP+unc-119(+) loxP]) II.
RW12174 C. elegans hlh-26(st12174[hlh-26::GFP + loxP + unc-119(+) + loxP] II; unc-119(tm4063) III. Show Description
hlh-26(st12174[hlh-26::GFP + loxP + unc-119(+) + loxP] II.
RW12175 C. elegans ast-1(st12175[ast-1::GFP + loxP + unc-119(+) + loxP] II; unc-119(tm4063) III. Show Description
ast-1(st12175[ast-1::GFP + loxP + unc-119(+) + loxP] II.
RW12178 C. elegans lin-26(st12178[lin-26::GFP + loxP + unc-119(+) + loxP] II; unc-119(tm4063) III. Show Description
lin-26(st12178[lin-26::GFP + loxP + unc-119(+) + loxP] II.
RW12320 C. elegans K11D2.4(st12320[K11D2.4::TY1::EGFP::3xFLAG]) I. Show Description
CRISPR/Cas9 engineered tagged endogenous locus.
RW20022 C. briggsae Cbr-unc-119(stIs20000) III; stIs20022. Show Description
stIs20022 [Cbr-his-72::GFP::pie-1 3'UTR + Cbr-unc-119(+)]. C. briggsae with fluorescently labeled (GFP) histones. Reference: Zhou Z, et al. Genetics. 2010 Mar; 184(3): 853–863. doi: 10.1534/genetics.109.110270 PMID: 20008572.
RW20025 C. briggsae Cbr-unc-119(stIs20000) III; stIs20025. Show Description
stIs20025 [Cbr-his-72::mCherry::pie-1 3'UTR + Cbr-unc-119(+)]. C. briggsae with fluorescently labeled (mCherry) histones. Reference: Zhou Z, et al. Genetics. 2010 Mar; 184(3): 853–863. doi: 10.1534/genetics.109.110270 PMID: 20008572.
RW3609 C. elegans unc-112(st581)/unc-39(e257) V. Show Description
Heterozgyotes are WT and segregate WT, Unc and Pats. Maintain by picking WT and checking for correct segregation of progeny.
SA1108 C. elegans tbb-1(tj59[tbb-2 substitution]) III. Show Description
The endogenous tbb-1 coding sequence was re-encoded with tbb-2 mimic sequence, expressing TBP-2 from the tbp-1 locus. Reference: Honda Y, et al. J Cell Sci. 2017 May 1;130(9):1652-1661. doi: 10.1242/jcs.200923. PMID: 28302908.
SA115 C. elegans unc-119(ed3) III; tjIs1. Show Description
tjIs1[pie-1::GFP::rho-1 + unc-119(+)]. GFP-RHO-1 is found at the basement membrane location in germline and early embryos and at the anterior cortical region in one-cell embyros. Small brood size. pID3.01-rho-1. Integration site unknown.
SA1171 C. elegans tba-4(tj63[gfp::TEV::3xFLAG::tba-4]) II. Show Description
GFP::TEV::3xFLAG tag inserted into the endogenous tba-2 locus. Reference: Nishida K, et al. Cell Struct. Funct. 2021 Jun 30;46(1):51-64. PMID: 33967119.
SA1176 C. elegans tba-7(tj66[gfp::TEV::3xFLAG::tba-7]) III. Show Description
GFP::TEV::3xFLAG tag inserted into the endogenous tba-7 locus. Reference: Nishida K, et al. Cell Struct. Funct. 2021 Jun 30;46(1):51-64. PMID: 33967119.
SA1180 C. elegans mec-12(tj70[gfp::TEV::3xFLAG::mec-12]) III. Show Description
GFP::TEV::3xFLAG tag inserted into the endogenous mec-12 locus. Reference: Nishida K, et al. Cell Struct. Funct. 2021 Jun 30;46(1):51-64. PMID: 33967119.
SA1205 C. elegans tbb-4(tj74[gfp::TEV::3xFLAG::tbb-4]) X. Show Description
GFP::TEV::3xFLAG tag inserted into the endogenous tbb-4 locus. Reference: Nishida K, et al. Cell Struct. Funct. 2021 Jun 30;46(1):51-64. PMID: 33967119.
SA1208 C. elegans mec-7(tj77[gfp::TEV::3xFLAG::mec-7]) X. Show Description
GFP::TEV::3xFLAG tag inserted into the endogenous mec-7 locus. Reference: Nishida K, et al. Cell Struct. Funct. 2021 Jun 30;46(1):51-64. PMID: 33967119.
SA125 C. elegans unc-119(ed3) III; tjIs4. Show Description
tjIs4 [pie-1::GFP::ect-2 + unc-119(+)]. GFP-ECT-2 is found at the anterior cortex in early embyros. pID3.01-ect-2. Integration site unknown. Maintain at 25C to avoid transgene silencing.
SA127 C. elegans unc-119(ed3) III; tjIs5. Show Description
tjIs5 [pie-1p::GFP::plk-1 + unc-119(+)]. GFP::PLK-1 is found at the mitotic centrosomes, kinetochores, and midbody in early embyros. pID3.01-plk-1. Integration site unknown. Maintain at 25C to avoid transgene silencing.
SA1280 C. elegans tbb-6(tj80[gfp::TEV::3xFLAG::tbb-6]) V. Show Description
GFP::TEV::3xFLAG tag inserted into the endogenous tbb-6 locus. Reference: Nishida K, et al. Cell Struct. Funct. 2021 Jun 30;46(1):51-64. PMID: 33967119.
SA1303 C. elegans tba-9(tj100[gfp::TEV::3xFLAG::tba-9]) X. Show Description
GFP::TEV::3xFLAG tag inserted into the endogenous tba-9 locus. Reference: Nishida K, et al. Cell Struct. Funct. 2021 Jun 30;46(1):51-64. PMID: 33967119.
SA131 C. elegans unc-119(ed3) III; tjIs6. Show Description
tjIs6 [pie-1p::GFP::cdc-42 + unc-119(+)]. GFP::CDC-42 localizes to basement membranes in both embryos and germline, and to the anterior cortex of one-cell embryos. pID3.01-cdc-42. Integration site unknown.
SA1358 C. elegans tba-5(tj102[gfp::TEV::3xFLAG::tba-5]) I. Show Description
GFP::TEV::3xFLAG tag inserted into the endogenous tba-5 locus. Reference: Nishida K, et al. Cell Struct. Funct. 2021 Jun 30;46(1):51-64. PMID: 33967119.
SA1378 C. elegans ben-1(tj87[gfp::TEV::3xFLAG::ben-1]) III. Show Description
GFP::TEV::3xFLAG tag inserted into the endogenous ben-1 locus. Reference: Nishida K, et al. Cell Struct. Funct. 2021 Jun 30;46(1):51-64. PMID: 33967119.
SA1386 C. elegans tba-6(tj105[gfp::TEV::3xFLAG::tba-6]) I. Show Description
GFP::TEV::3xFLAG tag inserted into the endogenous tba-6 locus. Reference: Nishida K, et al. Cell Struct. Funct. 2021 Jun 30;46(1):51-64. PMID: 33967119.
SA141 C. elegans unc-119(ed3) III; tjIs8. Show Description
tjIs8 [pie-1p::GFP::ebp-1 + unc-119(+)]. GFP::ebp-1 is found at the tips of growing microtubules in early embryos. pID3.01-ebp-1. Integration site unknown.
SA1430 C. elegans tba-8(tj108[gfp::TEV::3xFLAG::tba-8]) X. Show Description
GFP::TEV::3xFLAG tag inserted into the endogenous tba-8 locus. Reference: Nishida K, et al. Cell Struct. Funct. 2021 Jun 30;46(1):51-64. PMID: 33967119.
SA250 C. elegans tjIs54; tjIs57. Show Description
tjIs54 [pie-1p::GFP::tbb-2 + pie-1p::2xmCherry::tbg-1 + unc-119(+)]. tjIs57 [pie-1p::mCherry::his-48 + unc-119(+)]. Maintain at 25C to avoid transgene silencing. Reference: Toya M, et al. Methods Cell Biol. 2010;97:359-72.
SBP67 C. elegans spat-3(mgw14[R209Q]) X. Show Description
Egl, with defects in HSN migration, and HSN and PVQ axon guidance. Crispr/Cas9 induced mutation in Ring1 domain with epigenetic effects on neurodevelopment. Amino acid substitution that likely disrupts interaction with a specific substrate, resulting in a phenotype comparable to a complete gene deletion. Reference: Pierce SB, et al. Proc Natl Acad Sci U S A. 2018 Feb 13;115(7):1558-1563.
SBP74 C. elegans spat-3(mgw26) X. Show Description
Egl, with defects in HSN migration, and HSN and PVQ axon guidance. Crispr/Cas9 induced deletion. Reference: Pierce SB, et al. Proc Natl Acad Sci U S A. 2018 Feb 13;115(7):1558-1563.
SBW115 C. elegans lem-2(sbw5[lem-2::mNeonGreen]) II. Show Description
mNeonGreen tag inserted at C-terminus of endogenous lem-2 locus using self-exising drug selection casette method (described by Hastie et al., 2019 J Microbiol Biol Educ). Reference: Barger SR, et al. J Cell Sci 1 November 2023; 136 (21): jcs261385. doi: https://doi.org/10.1242/jcs.261385. PMID: 37795681.
SD1135 C. elegans +/szT1 [lon-2(e678)] I; dlg-1(ok318)/szT1 X. Show Description
SD1144 C. elegans pha-1(e2123) III; gaEx[F41H108.8p::GFP + pha-1(+)]. Show Description
Array name unknown. Maintain at 20-25C to select for transgenic animals.
SD1146 C. elegans pha-1(e2123) III; gaEx[B0218.8::GFP + pha-1(+)]. Show Description
Array name unknown. Maintain at 20-25C to select for transgenic animals.
SD1149 C. elegans pha-1(e2123) III; gaEx[zk970.2p::GFP + pha-1(+)]. Show Description
Array name unknown. Maintain at 20-25C to select for transgenic animals.
SD1333 C. elegans ccIs4251 I; stIs10047. Show Description
ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. stIs10047 [unc-14p::his-24::mCherry + unc-119(+)]. Reference: Liu, X, et al., Cell (2009).
SD1340 C. elegans ccIs4251 I; stIs10035. Show Description
ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. stIs10035 [eft-3p::HIS-24::mCherry + unc-119(+)]. Reference: Liu, X, et al., Cell (2009).
SD1345 C. elegans ccIs4251 I; stIs10077. Show Description
ccIs4251 [(pSAK2) myo-3p::GFP::LacZ::NLS + (pSAK4) myo-3p::mitochondrial GFP + dpy-20(+)] I. stIs10077 [pha-4p(I2L)::his-24::mCherry + unc-119(+)]. Reference: Liu, X, et al., Cell (2009).