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Strain Species Genotype
JM311 C. elegans lem-2(ca19) II. Show Description
Overall healthy but reduced brood size and pharyngeal pumping rate. Synthetic lethal with emr-1(-). ca19 is a Leu to Arg mutation at position 16 of LEM-2, reconstituting a mutation in the American Hutterite Population that causes juvenile cataracts and premature cardiomyopathy.
BN224 C. elegans lem-2(tm1582) bqSi210 II. Show Description
bqSi210 [lem-2p::lem-2::GFP + unc-119(+)] II. Might contain unc-119(ed3) in the background. Single-copy lem-2::GFP transgene under control of lem-2 regulatory sequences. lem-2(tm1582) embryos arrest when emr-1 is depleted by RNAi; bqSi210 fully rescues this phenotype. Reference: Morales-Martínez A, Dobrzynska A, Askjaer P. J Cell Sci. 2015 Feb 4.
VC1317 C. elegans lem-2(ok1807) II. Show Description
W01G7.5. Superficially wild type. External left primer: CGACCGAAATCACGAGAAAT. External right primer: CAGACGGAAGCACAAGAACA. Internal left primer: TCGAGACTCGCAGAGGATTT. Internal right primer: GTAGCGACGCGAGACTCATT. Internal WT amplicon: 2741 bp. Deletion size: 2169 bp. Deletion left flank: CTTAATTTCCGGTGAGATATTCTGTAGTTT. Deletion right flank: CTTTTCCTCGTCCCTCGTGAGGAAAAAGTTGCAGTGTCGG. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
BN147 C. elegans emr-1(gk119) I; bqSi142 II. Show Description
bqSi142 [emr-1p::emr-1::mCherry + unc-119(+)] II. Might contain unc-119(ed3) in the background. Single-copy emr-1::mCherry transgene under control of emr-1 regulatory sequences. emr-1(gk119) embryos arrest when lem-2 is depleted by RNAi; bqSi142 fully rescues this phenotype. Reference: Morales-Martínez A, Dobrzynska A, Askjaer P. J Cell Sci. 2015 Feb 4.
BN228 C. elegans bqSi210 II; bqSi226 IV. Show Description
bqSi210 [lem-2p::lem-2::GFP + unc-119(+)] II. bqSi226 [emr-1p::emr-1::mCherry + unc-119(+)] IV. Might contain unc-119(ed3) in the background. Single-copy lem-2::GFP transgene under control of lem-2 regulatory sequences and emr-1::mCherry under control of emr-1 regulatory sequences. Both transgenes are ubiquitously expressed and able to rescue development of animals without expression of endogenous lem-2 and emr-1. Reference: Morales-Martínez A, Dobrzynska A, Askjaer P. J Cell Sci. 2015 Feb 4.
BN243 C. elegans bqSi235 II; bqSi242 IV. Show Description
bqSi235 [emr-1p::emr-1::GFP + unc-119(+)] II. bqSi226 [lem-2p::lem-2::mCherry + unc-119(+)] IV. Might contain unc-119(ed3) in the background. Single-copy emr-1::GFP transgene under control of emr-1 regulatory sequences and lem-2::mCherry under control of lem-2 regulatory sequences. Both transgenes are ubiquitously expressed and able to rescue development of animals without expression of endogenous lem-2 and emr-1. Reference: Morales-Martínez A, Dobrzynska A, Askjaer P. J Cell Sci. 2015 Feb 4.
GW1539 C. elegans gwSi34 II; met-2(gw1419[met-2::FLAG::TEV::mCherry]) III. Show Description
gwSi34 [lem-2p::lem-2::GFP-3xFlag::lem-2 3'UTR] II. Superficially wild-type. Endogenously tagged met-2 locus. LEM-2::GFP is detectable at the nuclear periphery, and MET-2::mCherry signal detectable throughout nucleoplasm of all germline and somatic tissues. Reference: Delaney CE, et al. J Cell Biol. 2019 Mar 4;218(3):820-838. PMID: 30737265
OD83 C. elegans unc-119(ed3) III; ltIs37 IV; qaIs3507. Show Description
ltIs37 [(pAA64) pie-1p::mCherry::his-58 + unc-119(+)] IV. qaIs3507 [pie-1::GFP::LEM-2 + unc-119(+)].
XA3507 C. elegans unc-119(ed3) qaIs3507 III. Show Description
qaIs3507 [unc-119(+) + pie-1p::GFP::lem-2] WT. Stable expression of GFP::LEM-2 when grown at 20C. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. Commercial requests should be addressed to info@embl-em.de