Laboratory Information

NameUP View on WormBase
Allele designationcs
HeadMeera Vedavalli Sundaram
InstitutionUniversity of Pennsylvania, Philadelphia, PA
Address 445 Clinical Research Building
Dept of Genetics, Perelman School of Medicine
415 Curie Blvd
Philadelphia 19104-6145
United States
Website http://www.med.upenn.edu/camb/faculty/ggr/sundaram.html
Gene classes ave  cnk  ekl  eor  lpr  lron 

Strains contributed by this laboratory

Strain Genotype Species Description
HP17 sos-1(pd10) V. C. elegans sos-1 gain-of-function allele originally isolated as a suppressor of sem-5(n1619) lethality. pd10 causes a E99K substitution within the N-terminal histone fold domain of SOS-1. Single mutants appear wild-type.
HP23 sos-1(pd9) V. C. elegans sos-1(pd9) is a gain-of-function allele causing a C662Y substitution within the Ras exchange motif of SOS-1. Originally isolated as a suppressor of sem-5(n1619) lethality. Single mutants appear wild-type. Reference: Wooller A & Hopper N. (2014) European Worm Meeting. (Anyone using this allele may cite Neil Hopper as a personal communication based on this meeting abstract.)
MH575 lin-45(ku51) dpy-20(e1282) IV. C. elegans Weak hypomorphic allele of lin-45. Animals are Dpy but otherwise appear normal.
MH620 lin-45(ku112) dpy-20(e1282) IV. C. elegans Weak hypomorphic allele of lin-45. Animals are Dpy but otherwise appear normal. Occasional larval lethality.
UP1135 csEx52. C. elegans csEx52[hsp::lin-45AA + sur-5::GFP]. Maintain by picking GFP+.
UP1136 csEx53. C. elegans csEx53[hsp::lin-45AA + sur-5::GFP]. Maintain by picking GFP+.
UP1153 csEx63. C. elegans csEx63 [(pMS88) hsp16-41::torso^4021-Draf + (pTG96) sur-5::GFP]. Pick GFP+ to maintain. Muv phenotype in GFP+ after heat shock during larval stage. References: Kao G, et al. Development. 2004 Feb;131(4):755-65. Rocheleau CE, et al. Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11757-62.
UP1226 csEx72. C. elegans csEx72[hsp::lin-45ED + sur-5::GFP]. Maintain by picking GFP+.
UP1321 lpr-1(cs73) I. C. elegans Most animals die as rod-like lethal L1 larvae. Grow at 20C.
UP148 sem-5(cs15) X. C. elegans Truncation allele of sem-5 with complex behavior. About 15% larval lethal, about 75% Egl/Vul. Synthetic Muv in gap-1 background.
UP233 eor-1(cs28) IV. C. elegans Deletion allele. Mildly Unc, low percentage larval lethal, low percentage Egl.
UP2436 lpr-1(cs207) I. C. elegans cs207 is an early nonsense allele (Q72stop). Approximately 90% rod-like larval lethal, with 10% normal escapers. Exact percentages are sensitive to temperature and culture conditions.
UP2813 csSi3 [lin-3::lin-3S + unc-119(+)] II; lin-3(n1059) IV/nT1[qIs51] (IV;V) C. elegans lin-3(-) heterozygous balanced strain containing single copy MOS-mediated insertion of csSi1 transgene encoding lin-3S (short) splice isoform, expressed under control of the lin-3 promoter. The transgene rescues lethal and Vulvaless phenotypes (but not sterility) of lin-3 mutants, which can be recognized by absence of myo-2::GFP from the nT1 balancer. Pick GFP+ to maintain.
UP2814 csSi1 [lin-3::lin-3L + unc-119(+)] II; lin-3(n1059) IV/nT1[qIs51] (IV;V) C. elegans lin-3(-) heterozygous balanced strain containing single copy MOS-mediated insertion of csSi1 transgene encoding lin-3L (long) splice isoform, expressed under control of the lin-3 promoter. The transgene partially rescues lethal and Vulvaless phenotypes (but not sterility) of lin-3 mutants, which can be recognized by absence of myo-2::GFP from the nT1 balancer. Pick GFP+ to maintain.
UP2815 csSi2 [lin-3::lin-3XL + unc-119(+)] II; lin-3(n1059) IV/nT1[qIs51] (IV;V) C. elegans lin-3(-) heterozygous balanced strain containing single copy MOS-mediated insertion of csSi1 transgene encoding lin-3XL (extra long) splice isoform, expressed under control of the lin-3 promoter. The transgene partially rescues lethality (but not Vulvaless or sterile phenotypes) of lin-3 mutants, which can be recognized by absence of myo-2::GFP from the nT1 balancer. Pick GFP+ to maintain.
UP3542 lpr-3(cs231) X; csEx436. C. elegans csEx436 [lpr-3 (fosmid WRM619dE09) + myo-2p::mCherry]. Pick mCherry+ animals to maintain. cs231 is a Crispr/Cas9-induced null allele of lpr-3: a 13 nucleotide deletion in exon 1 results in frameshift. Homozygous mutants are embryonic lethal, but are rescued by csEx436 containing lpr-3(+) fosmid WRM619dE09. NOTE: lpr-3(cs231) should be considered the canonical allele as ok2351 also perturbs expression of adjacent gene lpr-6. Reference: Forman-Rubinsky R, Cohen JD and Sundaram MV. Genetics. 2017 Oct;207(2):625-642.
UP3666 lpr-3(cs250[ssSfGFP::lpr-3]) X​. C. elegans Endogenous lpr-3 locus tagged with ssSfGFP using CRISPR/Cas9. Superficially wild-type. Reference: Cohen JD, et al. Elife. 2020 Sep 25;9:e57874. PMID: 32975517.
UP3746 let-653(cs262[let-653::SfGFP]) IV. C. elegans Endogenous let-653 locus tagged with SfGFP using CRISPR/Cas9. Superficially wild-type. Reference: Cohen JD, et al. Elife. 2020 Sep 25;9:e57874. PMID: 32975517.
UP3756 let-4(cs265[ssmCherry::let-4]) X. C. elegans Endogenous let-4 locus tagged with mCherry using CRISPR/Cas9. mCherry expression is faint. Superficially wild-type. Reference: Cohen JD, et al. Elife. 2020 Sep 25;9:e57874. PMID: 32975517.
UP533 eor-2(cs42) X. C. elegans Nonsense allele. Mildly Unc, low percentage larval lethal, low percentage Egl.
UP604 sos-1(cs41) V. C. elegans Temperature sensitive missense allele. Larval lethal and Vul at 25C. About WT at 20C. Previously called let-341.
UP63 mat-3(ku233) III. C. elegans Weak hypomorphic allele. Cold-sensitive. Egl, Pvul and sterile at 20C.
UP725 mat-3(cs53)/unc-45(e286) III. C. elegans Heterozygotes are WT and segregate WT, Uncs, and Pvul which are sterile.
UP994 sur-6(sv30) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III). C. elegans sur-6(sv30) homozygotes are viable but segregate 100% dead embryos, and are GFP-. Weak Vulvaless and Unc. Maintain strain by picking GFP+ heterozygotes. qIs48 is an insertion of ccEx9747 (carries myo-2::GFP, pes-10::GFP, and a gut enhancer fused to GFP) onto the hT2 chromosome and is homozygous lethal. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype.
XW18042 qxIs722 II. C. elegans qxIs722 [dpy-7p::dpy-7::SfGFP (single copy)]. qxIs722 is a single copy insertion into ttTi5605 via CRISPR/Cas9. DPY-7::SfGFP expression in cuticle over hyp7. Reference: Miao R, et al. Dev Cell. 2020 Jan 6;52(1):21-37.e5.
XW5399 unc-76(e911) V; qxIs257. C. elegans qxIs257 [ced-1p::nuc-1::mCherry + unc-76(+)]. Integrated nuc-1::mCherry transgene marking lysosomes in epidermal cells. Site of chromosomal integration unknown. Not known if unc-76(e911) is still present in background. Reference: Li Y, et al. J Cell Biol. 2016 Oct 24;215(2):167-185.
XW8056 unc-76(e911) V; qxIs430. C. elegans qxIs430 [scav-3::GFP + unc-76(+)]. Integrated GFP translational fusion transgene marking lysosomes in epithelial cells. Not known if unc-76(e911) is still present in background. Reference: Li Y, et al. J Cell Biol. 2016 Oct 24;215(2):167-185.

Alleles contributed by this laboratory

Allele Type DNA Change Protein Change
cs73 Allele substitution
cs15 Allele substitution nonsense
cs28 Allele deletion
cs42 Allele substitution nonsense
cs41 Allele substitution
cs53 Allele substitution nonsense