| CB3844 |
C. elegans |
fem-3(e2006) IV. Show Description
Temperature sensitive Fem. Hermaphrodites at 15C, female at 25C. Maintain at 15C.
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| CB4015 |
C. elegans |
fem-3(e1999) IV. Show Description
Weakest known fem-3 mutation. Viable non-temperature-sensitive self-fertile XX hermaphrodites. e1999 XO males are slightly feminized, sometimes male-fertile. Reference: Hodgkin (1986) PMID: 3770465.
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| JK1973 |
C. elegans |
fem-3(q96) IV. Show Description
Semi-dominant Mog. Temperature-sensitive sterile. Maintain at 15C. Masculization of germline (Mog) at 25C.
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| JK654 |
C. elegans |
fem-3(q23) IV. Show Description
Maintain at 15C. Do not distribute this strain; other labs should request it from the CGC.
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| JK816 |
C. elegans |
fem-3(q20) IV. Show Description
Gain of function. Temperature sensitive. XX germline makes only sperm at 25C; XX germline makes oocytes and excess sperm at 15C. Do not distribute this strain; other labs should request it from the CGC.
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| CP87 |
C. briggsae |
Cbr-fem-3(nm63) IV. Show Description
XO are self-fertile hermaphrodites with low brood size and some somatic gonad defects. XX animals have normal brood size.
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| JK551 |
C. elegans |
unc-5(e53) fem-3(q22) IV. Show Description
Temperature sensitive. At 25C, XX germline makes only sperm; at 15C, germline makes oocytes and excess sperm. Unc. Do not distribute this strain; other labs should request it from the CGC.
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| JK860 |
C. elegans |
fem-3(q20) IV; fog-2(q71) V. Show Description
Temperature-sensitive. Maintain at 15 C. Do not distribute this strain; other labs should request it from the CGC.
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| CB3843 |
C. elegans |
fem-3(e1996)/unc-24(e138) dpy-20(e1282) IV. Show Description
Heterozygotes are WT and segregate WT, DpyUnc and fertile females.
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| CP89 |
C. briggsae |
Cbr-fem-2(nm27) III; Cbr-fem-3(nm63) IV. Show Description
XX are self-fertile hermaphrodites.
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| CB4760 |
C. elegans |
fem-1(e2044) mor-2(e1125) unc-24(e158) fem-3(q20) / unc-5(e53) dpy-20(e1282) IV Show Description
Wild-type hermaphrodites segregating wild-type hermaphrodites, Unc-24 females, Unc Dpy hermaphrodites. Maintain by picking wild-type hermaphrodites. Deletion allele of fem-1, with unusual maternal effect. Reference: Spence AM, et al. Cell. 1990 Mar 23;60(6):981-90. Johnson CL & Spence AM.. Science. 2011 Sep 2;333(6047):1311-4.
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| JK3965 |
C. elegans |
fbf-1(ok91) fbf-2(q704)/mIn1 [mIs14 dpy-10(e128)] II; fem-3(e1996)/qIs24 IV. Show Description
qIs24 [lag-2p::GFP]. Maintain by picking individual animals with green DTCs and green pharynx and scoring offspring for Fems and Fbfs. fem-3 is not well balanced by qIs24. fbf-1 fbf-2 homozygotes are germline proliferation defective and make only sperm. fem-3 homozygotes make only oocytes. fbf-1 fbf-3; fem-3 proliferates more than fbf-1 fbf-2 and makes only oocytes; also Muv. qIs24 contains lag-2::GFP; Distal Tip Cells are green; homozygotes are Rollers and heterozygotes Roll weakly. mIn1 is Dpy and GFP+ in the pharynx. Do not distribute this strain; other labs should request it from the CGC.
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| MOS370 |
C. elegans |
him-5(e1490) V; lite-1(ce314) X; fsIs22; ljIs114. Show Description
fsIs22 [osm-5p::fem-3::mCherry + unc-122p::GFP]. ljIs114 [gpa-13p?FLPase + sra-6p?FRT::mCherry::StopCodon::FRT?ChR2?YFP]. Him. Pan-ciliated masculinization with ChR2 specifically in ASH. Reference: Pechuk V., et al. 2022, Current Biology 32, 114. https://doi.org/10.1016/j.cub.2022.08.038
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| OH16790 |
C. elegans |
him-8(e1489) IV; lin-14(cc2841[lin-14::gfp]) X; otEx7578. Show Description
otEx7578 [rab-3p::fem-3::SL2::TagRFP + inx-6(prom18)::TagRFP]. Panneuronal overexpression of fem-3 causes panneuronal masculation. Reference: Sun H & Hobert O. Nature. 2021 Dec;600(7887):93-99. doi: 10.1038/s41586-021-04071-4. PMID: 34759317.
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| OH16837 |
C. elegans |
him-8(e1489) IV; nlp-45(ot1032[nlp-45::T2A::GFP::H2B]) X; otEx7578. Show Description
otEx7578 [rab-3p::fem-3::SL2::TagRFP + inx-6(prom18)::TagRFP]. Panneuronal overexpression of fem-3 causes panneuronal masculation. Reference: Sun H & Hobert O. Nature. 2021 Dec;600(7887):93-99. doi: 10.1038/s41586-021-04071-4. PMID: 34759317.
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| PS8509 |
C. elegans |
syIs640. Show Description
syIs640 [15xUAS::fem-3::mCherry::let-858 3'UTR + ttx-3p:RFP + 1kb DNA ladder (NEB)] cGAL effector to induce masculinization
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