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Strain Species Genotype
BS553 C. elegans fog-2(oz40) V. Show Description
Male/female strain. Maintain by mating.
CB4108 C. elegans fog-2(q71) V. Show Description
Male/female strain. Maintain by mating. XX animals are female. XO animals are WT males.
JK574 C. elegans fog-2(q71) V. Show Description
Male-female strain. Maintain by mating. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be used for any commercial purpose or for work on human subjects.
JH3644 C. elegans fog-2(q71) V; meg-3(ax4320[meg-3::mCherry]) X. Show Description
mCherry inserted at C terminus of endogenous meg-3 locus. fog-2(q71) is male/female strain. Maintain by mating. XX animals are female. XO animals are WT males. Reference: Putnam A, et al. Nat Struct Mol Biol. 2019 Mar;26(3):220-226.
JH3657 C. elegans pgl-3(ax4300[pgl-3::mCherry]) fog-2(q71) V. Show Description
mCherry inserted at C terminus of endogenous pgl-3 locus. fog-2(q71) is male/female strain. Maintain by mating. XX animals are female. XO animals are WT males. Reference: Putnam A, et al. Nat Struct Mol Biol. 2019 Mar;26(3):220-226.
CB6121 C. elegans eT5 (X; III); fog-2(q71) V females and eT5 (X; III) / eT1(III; V); fog-2 / eT1 (fog-2 V; III) males Show Description
Male/female strain, propagate by crossing. Females are eT5 (III;X); fog-2(q71) V. Males are eT5 (III;X) / eT1(III;V); fog-2 / eT1 (fog-2 III;V). Strain with multichromosomal sex determination. Reference: Strain 21 in Hodgkin (2002) PMID: 12399387.
DG1604 C. elegans fog-2(q71) V; ceh-18(mg57) X. Show Description
Maintain by mating females with males.
DG2356 C. elegans glp-1(tn777) III; fog-2(oz40) V. Show Description
Temperature sensitive. Maintain at 15C.
DG2474 C. elegans glp-1(ar202) III; fog-2(oz40) V. Show Description
Temperature sensitive. Maintain at 15C.
DG2884 C. elegans daf-2(e1370) III; fog-2(oz40) V. Show Description
Male-female strain. Maintain by mating at 15 C.
DG3449 C. elegans sacy-1(tn1385) I; fog-2(oz40) V. Show Description
sacy-1(tn1385) partially suppresses fog-2(oz40) self-sterility. Reference: Kim S, et al. 2012 Genetics
JK860 C. elegans fem-3(q20) IV; fog-2(q71) V. Show Description
Temperature-sensitive. Maintain at 15 C. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
XM1003 C. elegans nmr-1(ak4) II; fog-2(q71) V. Show Description
Negative regulator of oocyte maturation. Male/female strain.
XM1012 C. elegans inx-22(tm1661) I; fog-2(q71) V. Show Description
Negative regulator of oocyte maturation. Male/Female strain.
AU166 C. elegans daf-16(mgDf47) I; fog-2(q71) V. Show Description
Temperature sensitive. Maintain by mating males & females at 15C. fog-2(q71) is male-female.
JK993 C. elegans unc-51(e1189) fog-2(q71)/let-?(q265) V. Show Description
Heterozygotes are WT and segregate WT, Unc Females and Lethals. Maintain by picking WT. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.
CB4734 C. elegans tra-2(e1095)/+ II; fog-2(q71) V; xol-1(y9) X. [XX females and tra-2; fog-2; xol-1 XX males] Show Description
Obligate male/female strain; maintain by crossing. Anatomically normal XX females and XX males. Male/female XX strain with sex determined by tra-2(+). Reference: Strain 6 in Hodgkin (2002) PMID: 12399387.
JK1521 C. elegans fog-2(q71) pha-4(q490)/stu-3(q265) rol-9(sc148) V. Show Description
Heterozygotes are WT and segregate WT, Females which lack a pharynx (arrest as embryos or L1) and Sterile Unc Rollers. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects. See also WBPaper00003770.
DG1612 C. elegans vab-1(dx31)/mIn1 [dpy-10(e128) mIs14] II; fog-2(q71) V. Show Description
Maintain by mating GFP+ females with GFP+ males.
DG1650 C. elegans vab-1(dx31)/mIn1 [dpy-10(e128) mIs14] II; fog-2(q71) V; ceh-18(mg57) X. Show Description
Maintain by mating GFP+ females with GFP+ males.
JH2163 C. elegans unc-119(ed3) III; axIs1564. Show Description
axIs1564 [pie-1p::GFP::fog-2 ORF::fog-2 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C.
JH2207 C. elegans unc-119(ed3) III; axIs1584. Show Description
axIs1584 [pie-1p::GFP::histone H2B::fog-2 3'utr + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C.
PB303 C. elegans Show Description
Caenorhabditis elegans from association with a terrestrial isopod, Porcellio scaber, that was obtained from Ward's Biological Supply. Species ID confirmed by mating tests with JK574 fog-2(q71). Caenorhabditis elegans wild isolate.
PB306 C. elegans Show Description
Caenorhabditis elegans from association with a terrestrial isopod, Porcellio scaber, that was obtained from Connecticut Valley Biological Supply. Species ID confirmed by mating tests with JK574 fog-2(q71). Caenorhabditis elegans wild isolate.
PX624 C. elegans fxSi1 I; spe-44(fx123[spe-44::degron]) IV; fog-2(fx111) V. Show Description
fxSi1 [pie-1p::TIR-1::mRuby::unc-54 3' UTR + loxP, I: 2851003] I. Degron tag was inserted into the endogenous spe-44 locus in the JU2526 wild isolate background, allowing auxin-inducible spermatogenesis arrest and reversible male sterility. Reference: Kasimatis, KR et al. (2020) Post-Insemination Selection Dominates Pre-Insemination Selection in Driving Male Competitive Ability. bioRxiv doi: https://doi.org/10.1101/2021.06.23.449605
PX631 C. elegans fxSi3 I; fxSi4 II; fog-2(q71) V. Show Description
fxSi3 [hsp-16.41p::PEEL-1::tbb-2 3' UTR + rpl-28p::mKate2::unc-54 3'UTR + rps-0p::HygR::unc-54 3' UTR, I:2851003] I. fxSi4 [hsp-16.41p::PEEL-1::tbb-2 3'UTR + loxP, II: 8420157] II. Heat-shock strain can be maintained at 20C without any issues. Degron tag was inserted into the endogenous spe-44 locus, allowing auxin-inducible spermatogenesis arrest and reversible male sterility. Heat-shock-induced expression of PEEL-1 will cause lethality in both sexes. Five generations of lab adaptation following genome editing, all in the CB4856 background. Reference: Kasimatis, KR et al. (2021) Post-Insemination Selection Dominates Pre-Insemination Selection in Driving Male Competitive Ability. bioRxiv doi: https://doi.org/10.1101/2021.06.23.449605
PX658 C. elegans fxSi1 I; fxSi4 II; fxSi6 III; spe-44(fx123[spe-44::degron]) IV; fog-2(fx111) V. Show Description
fxSi1 [pie-1p::TIR-1::mRuby::unc-54 3' UTR + loxP, I: 2851003] I. fxSi4 [hsp-16.41p::PEEL-1::tbb-2 3'UTR + loxP, II: 8420157] II. fxSi6 [hsp-16.41p::PEEL-1::tbb-2 3' UTR + rpl-28p::mKate2::unc-54 3'UTR + rps-0p::HygR::unc-54 3' UTR, III: 10158855] III. Heat-shock strain can be maintained at 20C without any issues. Degron tag was inserted into the endogenous spe-44 locus, allowing auxin-inducible spermatogenesis arrest and reversible male sterility. Heat-shock-induced expression of PEEL-1 will cause lethality in both sexes. fxSi4 was originally inserted in a CB4845 background, but has been sufficiently backcrossed so that PX658 is >98.5% JU2526 genetic background. Reference: Kasimatis, KR. et al. (2021) Post-Insemination Selection Dominates Pre-Insemination Selection in Driving Male Competitive Ability. bioRxiv doi: https://doi.org/10.1101/2021.06.23.449605