| OH11119 |
C. elegans |
otIs377; otEx4945. Show Description
otIs377 [myo-3p::mCherry]. otEx4945 [hsp16-2p::hlh-1::2xFLAG + rol-6(su1006)]. Rollers. Maintain at 15-20C; pick Rollers. Both otIs377 and otEx4945 were generated as complex arrays with digested bacterial genomic DNA.
|
|
| OH11319 |
C. elegans |
otIs412 X. Show Description
otIs412 [unc-3p::GFP + rgef-1p::DsRed] X. GFP expression in DA/DB/VA/VB class neurons. Reference: Kerk SY, et al. Neuron 2017 (in press).
|
|
| OH11350 |
C. elegans |
otIs235; otIs252; otEx5141. Show Description
otEx5141 [hsp::tbx-37 + hsp::tbx-38 + elt-2p::DsRed]. Maintain by picking animals with dsRed in intestinal nuclei. Transcription factors TBX-37 and TBX-38 are ectopically expressed under control of a heat shock-inducible promoter. otIs235 [che-1p::mCherry + rol-6(su1006)]. otIs252 [lsy-6(+)(fosmid)::YFP + rol-6(su1006)]. Rollers. YFP expression in ASEL.
|
|
| OH11411 |
C. elegans |
pha-1(e2123) III; otEx5174. Show Description
otEx5174 [unc-31(fosmid)::SL2::NLS::YFP::H2B + pha-1(+)]. Maintain at 25C to select for array. Pan-neuronal nuclear YFP expression. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
|
|
| OH11746 |
C. elegans |
pha-1(e2123) III; otIs447 IV. Show Description
otIs447 [unc-3p::mCherry + pha-1(+)] IV. DA/DB/VA/VB motor neuron class-specific red fluorescent reporter. Reference: Kerk SY, et al. Neuron 2017 (in press).
|
|
| OH12412 |
C.elegans |
otIs502. Show Description
otIs502 [hlh-2(fosmid)::YFP + myo-3p::mCherry]. Broad embryonic expression; later in development transgene is expressed in DTCs, vulva, and some gland cells in pharynx. Reference: Masoudi N, et al. PLoS Biol. 2018 Apr 19;16(4):e2004979.
|
|
| OH12500 |
C. elegans |
otEx5664. Show Description
otEx5664 [oig-1(fosmid)::GFP + ttx-3p::mCherry]. Pick mCherry+ animals to maintain. Fosmid-based oig-1 transcritpional reporter.
|
|
| OH12644 |
C. elegans |
otEx5765. Show Description
otEx5765 [lin-14(fosmid)::GFP + ttx-3p::mCherry]. Pick mCherry+ animals to maintain. mCherry might be difficult to see at lower magnification. Fosmid-based lin-14 transcritpional reporter; see Li and Greenwald, Current Biology 2010, Oct 26;20(20):1875-9.
|
|
| OH12738 |
C. elegans |
otIs545; otIs593. Show Description
otIs545 [gcy-5p::RFP + LacO repeats]. otIs593 [let-858p::GFP::LacI + ttx-3p::mCherry]. Maintain at 20C; otIs545 is very unstable at lower temperatures. RFP expression in ASER neuron. LACI::GFP aggregates on LacO repeats to form two dots in every nucleus. otIs545 is unstable, presumably due to the highly repetitive nature of the LacO array. Stock should be periodically examined for RFP expression and GFP localization to retain expression of the array. Reference: Patel T & Hobert O. eLife 2017.
|
|
| OH12849 |
C. elegans |
pha-1(e2123) III; otIs356 V; otEx5886. Show Description
otIs356 [rab-3p(prom1)::2xNLS::TagRFP] V. otEx5886 [unc-57(fosmid)::SL2::NLS::YFP::H2B + pha-1(+)]. Maintain at 25C to select for array. Pan-neuronal nuclear YFP expression. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
|
|
| OH12860 |
C. elegans |
pha-1(e2123) III; otIs356 V; otEx5897. Show Description
otIs356 [rab-3p(prom1)::2xNLS::TagRFP] V. Pan-neuronal nuclear RFP expression. otEx5897 [egl-21(fosmid)::SL2::NLS::YFP::H2B + pha-1(+)]. Maintain at 25C to select for array. Pan-neuronal nuclear YFP expression. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
|
|
| OH12863 |
C. elegans |
pha-1(e2123) III; otIs356 V; otEx5900. Show Description
otIs356 [rab-3p(prom1)::2xNLS::TagRFP] V. otEx5900 [tbb-4(fosmid)::SL2::NLS::YFP::H2B + pha-1(+)]. Maintain at 25C to select for array. Neuronal nuclear YFP expression. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
|
|
| OH12888 |
C. elegans |
pha-1(e2123) III; otIs356 V; otEx5924. Show Description
otIs356 [rab-3p(prom1)::2xNLS::TagRFP] V. otEx5924 [snt-1(fosmid)::SL2::NLS::YFP::H2B + pha-1(+)]. Maintain at 25C to select for otEx5924. Pan-neuronal nuclear YFP expression. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
|
|
| OH12889 |
C. elegans |
pha-1(e2123) III; otIs356 V; otEx5925. Show Description
otIs356 [rab-3p(prom1)::2xNLS::TagRFP] V. otEx5925 [unc-18(fosmid)::SL2::NLS::YFP::H2B + pha-1(+)]. Maintain at 25C to select for array. Pan-neuronal nuclear YFP expression. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
|
|
| OH13098 |
C. elegans |
che-1(ot75) I. Show Description
che-1(ot75) is a null allele caused by an early STOP codon in exon 1. Reference: Chang S, et al. Genes Dev. 2003 Sep 1;17(17):2123-37.
|
|
| OH13099 |
C. elegans |
otIs583. Show Description
otIs583 [gcy-5p*::GFP + ttx-3p::mCherry]. Robust GFP expression in both ASE neurons. mCherry expression in AIY meurons. *This strain contains a short gcy-5 promoter with the CHE-1 binding ASE motif multimerized. GFP expression is strong enough to visualize morphology of both ASE neurons simultaneously. Reference: Patel T & Hobert O. eLife 2017. Etchberger JF, et al. Development. 2009 Jan;136(1):147-60.
|
|
| OH13102 |
C. elegans |
otIs586 X. Show Description
otIs586 [gcy-6(fosmid)::SL2::NLS::GFP + ttx-3p::mCherry] X. Reporter tag inserted into gcy-6(+) fosmid; expressed only in the ASEL neuron during normal growth conditions. Reference: Patel T & Hobert O. eLife 2017.
|
|
| OH13421 |
C. elegans |
pha-1(e2123) III; otIs356 V; otEx6254. Show Description
otIs356 [rab-3p(prom1)::2xNLS::TagRFP] V. otEx6254 [snb-1(fosmid)::SL2::NLS::YFP::H2B + pha-1(+)]. Maintain at 25C to select for otEx6254. Ubiquitous nuclear YFP expression. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
|
|
| OH13422 |
C. elegans |
pha-1(e2123) III; otIs356 V; otEx6255. Show Description
otIs356 [rab-3p(prom1)::2xNLS::TagRFP] V. otEx6255 [unc-31(fosmid)::SL2::NLS::YFP::H2B + pha-1(+)]. Maintain at 25C to select for otEx6254. Ubiquitous nuclear YFP expression. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
|
|
| OH13423 |
C. elegans |
pha-1(e2123) III; otIs356 V; otEx6256. Show Description
otIs356 [rab-3p(prom1)::2xNLS::TagRFP] V. otEx6256 [unc-10a(fosmid)::SL2::NLS::YFP::H2B + pha-1(+)]. Maintain at 25C to select for otEx6256. Pan-neuronal nuclear YFP expression. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
|
|
| OH13425 |
C. elegans |
pha-1(e2123) III; otIs356 V; otEx6258. Show Description
otIs356 [rab-3p(prom1)::2xNLS::TagRFP] V. otEx6258 [unc-108(fosmid)::SL2::NLS::YFP::H2B + pha-1(+)]. Maintain at 25C to select for otEx6258. Ubiquitous nuclear YFP expression. Reference: Stefanakis N., Carrera I., Neuron. 2015 Aug 19;87(4):733-50.
|
|
| OH13426 |
C. elegans |
pha-1(e2123) III; otIs356 V; otEx6259. Show Description
otIs356 [rab-3p(prom1)::2xNLS::TagRFP] V. otEx6259 [unc-10B(fosmid)::SL2::NLS::YFP::H2B + pha-1(+)]. Maintain at 25C to select for otEx6259. Pan-neuronal nuclear YFP expression. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50.
|
|
| OH13787 |
C. elegans |
otEx6368. Show Description
otEx6368 [GFP::oig-8(fosmid) + ttx-3p::mCherry]. Pick ttx-3p::mCherry to maintain. Fosmid-based oig-8 translational reporter (GFP inserted in N-terminus of OIG-8 after peptide sequence).
|
|
| OH13851 |
C. elegans |
pha-1(e2123) III; him-5(e1490) V; otEx6415. Show Description
otEx6415 [sri-36p::GFP + pha-1(+)]. Maintain at 25C to select for transgenic array. Reference: Vidal B, et al. (2018) PLoS Biol 16(1): e2004218.
|
|
| OH13853 |
C. elegans |
pha-1(e2123) III; him-5(e1490) V; otEx6417. Show Description
otEx6417 [sri-39p::GFP + pha-1(+)]. Maintain at 25C to select for transgenic array. Reference: Vidal B, et al. (2018) PLoS Biol 16(1): e2004218.
|
|
| OH13867 |
C. elegans |
pha-1(e2123) III; him-5(e1490) V; otEx6431. Show Description
otEx6431 [srx-3p::GFP + pha-1(+)]. Maintain at 25C to select for transgenic array. Reference: Vidal B, et al. (2018) PLoS Biol 16(1): e2004218.
|
|
| OH13885 |
C. elegans |
pha-1(e2123) III; him-5(e1490) V; otEx6449. Show Description
otEx6449 [sru-30p::GFP + pha-1(+)]. Maintain at 25C to select for transgenic array. Reference: Vidal B, et al. (2018) PLoS Biol 16(1): e2004218.
|
|
| OH14049 |
C. elegans |
otIs629; ntIs1 V. Show Description
otIs629 [gcy-7p::TagRFP + ttx-3p::GFP]. ntIs1 [gcy-5p::GFP + lin-15(+)] V. RFP expression in ASEL neurons. GFP expression appears in ASER (in adults) and AIY neurons. Reference: Patel T & Hobert O. eLife 2017.
|
|
| OH14230 |
C. elegans |
pha-1(e2123) III; him-5(e1490) V; otEx6607. Show Description
otEx6607 [srj-38p::GFP + pha-1(+)]. Maintain at 25C to select for transgenic array. Reference: Vidal B, et al. (2018) PLoS Biol 16(1): e2004218.
|
|
| OH14235 |
C. elegans |
pha-1(e2123) III; him-5(e1490) V; otEx6612. Show Description
otEx6612 [srsx-38p::GFP + pha-1(+)]. Maintain at 25C to select for transgenic array. Reference: Vidal B, et al. (2018) PLoS Biol 16(1): e2004218.
|
|
| OH14243 |
C. elegans |
pha-1(e2123) III; him-5(e1490) V; otEx6619. Show Description
otEx6619 [srg-31p::GFP + pha-1(+)]. Maintain at 25C to select for transgenic array. Reference: Vidal B, et al. (2018) PLoS Biol 16(1): e2004218.
|
|
| OH14244 |
C. elegans |
pha-1(e2123) III; him-5(e1490) V; otEx6620. Show Description
otEx6620 [srg-32p::GFP + pha-1(+)]. Maintain at 25C to select for transgenic array. Reference: Vidal B, et al. (2018) PLoS Biol 16(1): e2004218.
|
|
| OH14246 |
C. elegans |
pha-1(e2123) III; him-5(e1490) V; otEx6622. Show Description
otEx6622 [srg-39p::GFP + pha-1(+)]. Maintain at 25C to select for transgenic array. Reference: Vidal B, et al. (2018) PLoS Biol 16(1): e2004218.
|
|
| OH14271 |
C. elegans |
pha-1(e2123) III; him-5(e1490) V; otEx6634. Show Description
otEx6634 [str-31p::GFP + pha-1(+)]. Maintain at 25C to select for transgenic array. Reference: Vidal B, et al. (2018) PLoS Biol 16(1): e2004218.
|
|
| OH14368 |
C. elegans |
pha-1(e2123) III; him-5(e1490) V; otEx6710. Show Description
otEx6710 [srd-32p::GFP + pha-1(+)]. Maintain at 25C to select for transgenic array. Reference: Vidal B, et al. (2018) PLoS Biol 16(1): e2004218.
|
|
| OH14370 |
C. elegans |
pha-1(e2123) III; him-5(e1490) V; otEx6711. Show Description
otEx6711 [srsx-37p::GFP + pha-1(+)]. Maintain at 25C to select for transgenic array. Reference: Vidal B, et al. (2018) PLoS Biol 16(1): e2004218.
|
|
| OH14420 |
C. elegans |
pals-22(ot811) III; otIs381 V; otEx6761. Show Description
otIs381 [ric-19p(prom6)::2xNLS::GFP + elt-2::DsRed] V. Pan-neuronal nuclear GFP expression. otEx6761 [pals-22(+) + ttx-3p::mChOPTI]; derived from PCR fragment containing the genomic pals-22 locus. pals-22(ot811) mutant shows transgene-silencing phenotype; otEx6761 rescues ot811 and restores otIs381 expression at wild-type levels. Reference: Stefanakis N., Carrera I., Hobert O. Neuron. 2015 Aug 19;87(4):733-50. Leyva-Díaz E, et al. Genetics (2017), 207(2):529-545.
|
|
| OH14431 |
C. elegans |
otIs638. Show Description
otIs638 [unc-6(fosmid)::NLS::YFP::H2B + ttx-3p::mCherry + pha-1(+)]. Integrated fosmid reporter for unc-6. Reference: Weinberg P, et al. Curr Biol. 2018 Feb 19;28(4):623-629.e3.
|
|
| OH14454 |
C. elegans |
otIs587; otIs304. Show Description
otIs587 [gcy-5(fosmid)::SL2::NLS::GFP + ttx-3p::mCherry]. otIs304 [hsp16-2p::che-1::3xHA::BLRP + rol-6(su1006)]. Rollers. GFP reporter tag inserted into gcy-5(+) fosmid. gcy-5 is normally expressed in ASER and RIGL/R neurons, but upon heatshock, expression of CHE-1 induces ectopic gcy-5 expression (the extent of ectopic expression is dependent on the timing of the heatshock). Reference: Patel T & Hobert O. eLife 2017.
|
|
| OH14545 |
C. elegans |
pha-1(e2123) III; otEx6801. Show Description
otEx6801 [lgc-38p::GFP + pha-1(+)]. Maintain at 25C to select for array. PCR used to fuse 3.9 kb lgc-38 promoter with GFP reporter. Line 10. Reference: Gendrel M, et al. Elife. 2016 Oct 14;5.
|
|
| OH14546 |
C. elegans |
pha-1(e2123) III; otEx6802. Show Description
otEx6802 [lgc-37p::GFP + pha-1(+)]. Maintain at 25C to select for maintain array. Reporter contains 5 kb of lgc-37 promoter fused with GFP. Derived from injection of pMG249; line 5. Reference: Gendrel M, et al. Elife. 2016 Oct 14;5.
|
|
| OH14570 |
C. elegans |
otEx6820. Show Description
otEx6820 [ceh-36(prom3)::oig-8 + ttx-3p::mCherry]. Pick ttx-3p::mCherry to maintain.
|
|
| OH14750 |
C. elegans |
pha-1(e2123) III; him-5(e1490) V; otEx6853. Show Description
otEx6853 [srv-3p::GFP + pha-1(+)]. Maintain at 25C to select for transgenic array. Reference: Vidal B, et al. (2018) PLoS Biol 16(1): e2004218.
|
|
| OH14757 |
C. elegans |
pha-1(e2123) III; him-5(e1490) V; otEx6860. Show Description
otEx6860 [srv-32p::GFP + pha-1(+)]. Maintain at 25C to select for transgenic array. Reference: Vidal B, et al. (2018) PLoS Biol 16(1): e2004218.
|
|
| OH14758 |
C. elegans |
pha-1(e2123) III; him-5(e1490) V; otEx6861. Show Description
otEx6861 [srv-34p::GFP + pha-1(+)]. Maintain at 25C to select for transgenic array. Reference: Vidal B, et al. (2018) PLoS Biol 16(1): e2004218.
|
|
| OH14762 |
C. elegans |
pha-1(e2123) III; him-5(e1490) V; otEx6865. Show Description
otEx6865 [srm-3p::GFP + pha-1(+)]. Maintain at 25C to select for transgenic array. Reference: Vidal B, et al. (2018) PLoS Biol 16(1): e2004218.
|
|
| OH14767 |
C. elegans |
pha-1(e2123) III; him-5(e1490) V; otEx6870. Show Description
otEx6870 [srr-3p::GFP + pha-1(+)]. Maintain at 25C to select for transgenic array. Reference: Vidal B, et al. (2018) PLoS Biol 16(1): e2004218.
|
|
| OH14864 |
C. elegans |
kyIs140 I; oig-8(ot818) II; otTi24. Show Description
kyIs140: [str-2::GFP + lin-15(+)] I. otTi24 [ceh-36(prom3)::oig-8 + NeoR]. Single-copy mini-Mos insertion of rescuing ceh-36(prom3)::oig-8 transgene.
|
|
| OH14888 |
C. elegans |
daf-16(ot853[daf-16::mNG::3xFlag::AID*]) I; ieSi57 II; daf-2(e1370) III. Show Description
ieSi57 [eft-3p::TIR1::mRuby::unc-54 3'UTR] II. Maintain at 15C. Temperature-sensitive dauer constitutive. CRISPR/Cas9-engineered AID* conditional daf-16 allele in daf-2(e1370) background with ubiquitous TIR1 expression. Reference: Aghayeva U et al. PLoS Biol. 2021 Apr 23;19(4):e3001204. doi: 10.1371/journal.pbio.3001204. PMID: 33891586.
|
|
| OH14962 |
C. elegans |
pha-1(e2123) III; him-5(e1490) V; otEx6955. Show Description
otEx6955 [srz-32p::GFP + pha-1(+)]. Maintain at 25C to select for transgenic array. Reference: Vidal B, et al. (2018) PLoS Biol 16(1): e2004218.
|
|
