Strain Information
| Name | RG3429 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | gst-25 & spe-15(ve929[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP])/szT1 [lon-2(e678) umnIs61] I; +/szT1 X. |
| Description | umnIs61 [myo-2p::mKate2 + NeoR, X: 15420938 (intergenic)] I. Sterile. Deletion of 1754 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break in parental strain N2. Sterile phenotype is likely spe-15.Heterozygotes are wild-type GFP+ mKate2+, and segregate wild-type GFP+ mKate2+, sterile GFP+ non-mKate2 (ve929 homozygotes), Lon non-GFP mKate2+ males (szT1 hemizygotes), Lon GFP+ non-mKate2+ males (ve929 hemizygotes) and dead eggs (szT1 homozygotes and aneuploids). Maintain by picking wild-type GFP+ mKate2+. Maintain by picking wild-type GFP+ mKate2+. Left flanking Sequence: GAGACAAAATCTTCTGAATATCAAGTTCCT; Right flanking sequence: AGGAGAATTTAAGGGTCATTTTGATAAGTC. gst-25 sgRNA A: TGTGGATTACGTGCTCTTCG; gst-25 sgRNA B: GATACCGCGGGTTCCAAAGT. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation. |
| Mutagen | Crispr/Cas9 |
| Made by | RG KO Group |
| Laboratory | RG |
| Reference | n/a |
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