Search Strains

Pick wild-type to maintain. Segregates lethals. Dp/Dp are homozygous lethal.

LETHAL EARLY LARVAL Pick WT to maintain.

Lethal early larval. Maintain by picking WT.

LETHAL LATE LARVAL MATERNAL EFFECT Pick WT to maintain.

Pick WT to maintain. Throws WT, Sterile Uncs, and dead eggs(homozygous Dp).

LETHAL LATE LARVAL SEX-INFLUENCED Maintain by picking WT.

LETHAL EARLY LARVAL. Maintain by picking WT.

Pick wild-type to maintain. Segregates wild-type and lethals. mnDp1 duplication rescues unc-3 & let-2. mnDp1 is homozygous lethal.

LETHAL EARLY LARVAL. Maintain by picking WT.

FERTILITY POOR LETHAL EARLY LARVAL Maintain by picking WT.

LETHAL EARLY LARVAL. Maintain by picking WT.

LETHAL EARLY LARVAL. Maintain by picking WT.

LETHAL EARLY LARVAL. Maintain by picking WT.

LETHAL EMBRYONIC. WT strain.

LETHAL EARLY LARVAL. Maintain by picking WT. mn125 pka let-17.

LETHAL EARLY LARVAL. Maintain by picking WT.

NOT MALE-RESCUED MATERNAL EFFECT STERILE Maintain by picking WT.

LETHAL EARLY LARVAL. Maintain by picking WT.

GROWTH SLOW LETHAL EMBRYONIC ts(TEMPERATURE-SENS) See also CGC 1806.

LETHAL EARLY LARVAL. Maintain by picking WT.

Maintain by picking WT.

LETHAL EARLY LARVAL. Maintain by picking WT.

GROWTH SLOW. MALE-RESCUED. STERILE. Maintain by picking WT.

STERILE. Maintain by picking WT.

Pick WT to maintain. WT segregates DpyUnc and WT.

LETHAL EMBRYONIC RECESSIVE Pick WT to maintain. See also CGC 1806.

LETHAL EARLY LARVAL. Pick WT to maintain.

LETHAL EARLY LARVAL. Maintain by picking WT.

WT PHENOTYPE.

DPYISH. LETHAL EARLY LARVAL AT 25C. STERILE 20C.

LETHAL EMBRYONIC NULL ALLELE Maintain by picking WT. See also CGC 1806.

Rubberband Unc. Reverts. There is some question regarding e1500 in this strain. Please see MT19321 as a better strain.

Strain throws WT and Uncs. Maintain by picking WT.

Strain throws WT and Unc. Maintain by picking WT.

WT phenotype.

WT phenotype. Segregates Uncs which are Osm. [Osm phenotype checked 2/94.] mnDp3[sup-10(mn338)] and mnDp13 are the same. mnDp13 was derived from mnDp3 as lacking sup-10(+). It is not known if mnDp3 picked up a mutation in sup-10 or if the end of mnDp3 was shortened.

Strain throws WT and Unc. Maintain by picking WT.

ncIs2 [pH20::GFP + pBlueScript]. Heterozygotes are WT and segregate WT, Uncs, and animals with muscle attachment defects and ventral cord displacement and detachment which arrest in larval development. Not well balanced. Neurons visualized with ncIs2.

Heterozygotes are Unc-93 and segregate more Unc-93, yDf10 homozygotes (dead eggs) and Unc-93 Dpy-17 homozygotes (young dpy-17 larvae are easily recognizable as abnormal spindle-shaped things). Difficult to maintain and use. yDf10 apparently causes semi-sterility (a second strain constructed by the Mark Edgley at the CGC, yDf10/qC1, exhibits same sterility), and unc-93 is Egl and difficult to mate into. Some Df homozygotes are laid, but most remain inside the mother.

Heterozygotes are WT hermphrodites whose progeny include WT hermaphrodites, Dpy hermaphrodites (mnDp66; yDf13 unc-1 dpy-3), WT males and Dpy males.

WT hermaphrodites whose progeny include WT hermaphrodites, Dpy hermaphrodites (mnDp66; unc-1 dpy-3), Unc hermaphrodites (yDp14; unc-1 dpy-3), DpyTra hermaphrodites (mnDp66/yDp14; yDf17), WT males, Unc males, and Dpy males. There are 2 types of WT hermaphrodites in this strain which are indistinguishable unless you score their offspring: mnDp66/yDp14; him-8; yDf17/unc-1 dpy-3 animals will have many WT males progeny; but mnDp66/yDp14; him-8; unc-1 dpy-3 animals will have primarily dpy male progeny [mnDp66/yDp14; unc-1 dpy-3 XO animals are mostly dead, but there are some escapers of lethality]. Maintain by picking L4 WT hermaphrodites and checking for correct segregation of progeny.

This strain is homozygous for a deletion (gk804) in ZK185.2, detectable by PCR using the following primers. External left primer: AACCAAACGATGTCCCTGAC. External right primer: TCGGAAATGAAAACCCCATA. Internal left primer: TATTAGAGGCATATCGGCGG. Internal right primer: ATCACACCGGCGAGAATTAG. Internal WT amplicon: 1842 bp. Deletion size: 780 bp. Deletion left flank: ACTTGAATTTTTCCAGTAATTTTAGTTTTG. Deletion right flank: CTCTACCGGAGTTTGAAGGCACTTATTCGT. Validation: No CGH probes for gk804. Other deletion (gk3143) identified by CGH. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

This strain is homozygous for a deletion (gk1084) in ZK270.2, detectable by PCR using the following primers. External left primer: AATGGTGACACGATGCTCAA. External right primer: ACACAGACACAGCAAGACGG. Internal left primer: GTTAAATTCCAGTGGCTGCG. Internal right primer: GAAGCCGATGGACAAAGAGA. Internal WT amplicon: 796 bp. Deletion size: 179 bp. Deletion left flank: ATAATTTGCTAGTCTTTTTGGAATTTTTCT. Deletion right flank: GATTTAGGTATTTTAAAGTCGACGGACAAA. Validation: gk1084 passed by diagnostic PCR. No CGH probes for gk1084. Other deletions (gk3193, gkDf29) identified by CGH. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

T06C10.4, T06C10.6, T06C10.3, cTe154X.1. The gk1263 allele was identified by PCR and validated by CGH, and can be detected with PCR using the following primers. External left primer: GATGGAGTCGGTGGTGTTCT. External right primer: ATTTTTCAACTGCGAGCGAT. Internal left primer: GCTGGCAGTATTCGGATGAT. Internal right primer: AAATTTGCCGAAACGTGAAC. Internal WT amplicon: 2806 bp. Deletion size: 1036 bp. Deletion left flank: ACAAAATAAACGAGTTTAAAAAAACATTCA. Deletion right flank: TAACAAGGTACAATGGTTCCTGTAGTACTC. Other lesions identified by CGH. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

vkEx1093 [nhx-2p::mCherry::lgg-1]. Maintain by picking mCherry+ animals. Increased puncta under autophagy conditions. Reference: Gosai SJ, et al. PLoS One. 2010 Nov 12;5(11):e15460.

vkEx1243 [nhx-2p::ubiquitin-V::mCherry + myo-2p::GFP]. Increased Ub-tagged mCherry accumulation upon blockage of the proteosome by RNAi. Faint mCherry expression in intestine. GFP+ pharynx. References: Miedel MT, et al. PLoS One. 2012;7(7):e40145. Gosai SJ, et al. PLoS One. 2010 Nov 12;5(11):e15460. Dantuma NP, et al. Nat Biotechnol. 2000 May;18(5):538-43.

vkEx1244 [nhx-2p::ubiquitin-Met::mCherry + myo-2p::GFP]. mCherry behaves as an umodified cytosolic protein upon ubiquitin cleavage due to the absence of a degredation signal (N-terminal methionine). Diffuse mCherry expression in intestine. GFP+ pharynx. References: Miedel MT, et al. PLoS One. 2012;7(7):e40145. Gosai SJ, et al. PLoS One. 2010 Nov 12;5(11):e15460. Dantuma NP, et al. Nat Biotechnol. 2000 May;18(5):538-43.

Dpy and Vab (tail abnormalities).

Temperature sensitive embryonic lethal. Maintain at 15C. Received new stock 3/20/2008 from Mello lab. Replacement stock received from Mello Lab 01/14/2010.