umnIs25 [myo-2p::GFP + NeoR, X: 15420938 (intergenic)]. Pick wild-type GFP+ to maintain. Segregates lethals: homozygous Dp/Dp are lethal. Derived by insertion of myo-2p::GFP transgene into mnDp1 duplication in parental strain SP219 using CRISPR/Cas9.

Animals with Dp/+ are WT. Animals which have lost the Dp arrest as L1 with Gob (gut-obstructed) phenotype. Animals with Dp/Dp are sterile homozygotes. gob-1(ca17) is a small deletion, removing nine genes between R03A10.4 and H13N06.4 (inclusive) and possibly the seven additional genes F39D8.2 to R03A10.3 and H13N05.6.

Animals with the duplication are WT and segregate WT, sterile and Pats. Strain can be propagated by chunking.

Pick wild-type to maintain. Segregates lethals. Dp/Dp are homozygous lethal.

LETHAL EARLY LARVAL Pick WT to maintain.

Lethal early larval. Maintain by picking WT.

LETHAL LATE LARVAL MATERNAL EFFECT Pick WT to maintain.

Pick WT to maintain. Throws WT, Sterile Uncs, and dead eggs(homozygous Dp).

LETHAL LATE LARVAL SEX-INFLUENCED Maintain by picking WT.

LETHAL EARLY LARVAL. Maintain by picking WT.

Pick wild-type to maintain. Segregates wild-type and lethals. mnDp1 duplication rescues unc-3 & let-2. mnDp1 is homozygous lethal.

LETHAL EARLY LARVAL. Maintain by picking WT.

FERTILITY POOR LETHAL EARLY LARVAL Maintain by picking WT.

LETHAL EARLY LARVAL. Maintain by picking WT.

LETHAL EARLY LARVAL. Maintain by picking WT.

LETHAL EARLY LARVAL. Maintain by picking WT.

LETHAL EMBRYONIC. WT strain.

LETHAL EARLY LARVAL. Maintain by picking WT. mn125 pka let-17.

LETHAL EARLY LARVAL. Maintain by picking WT.

NOT MALE-RESCUED MATERNAL EFFECT STERILE Maintain by picking WT.

LETHAL EARLY LARVAL. Maintain by picking WT.

GROWTH SLOW LETHAL EMBRYONIC ts(TEMPERATURE-SENS) See also CGC 1806.

LETHAL EARLY LARVAL. Maintain by picking WT.

Maintain by picking WT.

LETHAL EARLY LARVAL. Maintain by picking WT.

GROWTH SLOW. MALE-RESCUED. STERILE. Maintain by picking WT.

STERILE. Maintain by picking WT.

WT PHENOTYPE

WT Phenotype. Gives dead eggs.

WT PHENOTYPE.

WT PHENOTYPE. Should throw dead eggs.

WT PHENOTYPE. This strain should throw dead eggs, but it is not doing so. Hasn't been checked to see if the deficiency is still there.

WT PHENOTYPE. Should throw dead eggs.

WT PHENOTYPE.

WT PHENOTYPE.

WT PHENOTYPE

WT PHENOTYPE

WT phenotype. Should throw dead eggs.

WT PHENOTYPE

WT PHENOTYPE

WT PHENOTYPE

WT PHENOTYPE.

WT PHENOTYPE.

WT PHENOTYPE

LETHAL EMBRYONIC RECESSIVE Pick WT to maintain. See also CGC 1806.

LETHAL EARLY LARVAL. Pick WT to maintain.

LETHAL EARLY LARVAL. Maintain by picking WT.

WT PHENOTYPE.

WT PHENOTYPE.

DPYISH. LETHAL EARLY LARVAL AT 25C. STERILE 20C.