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Strain Species Genotype
PR808 C. elegans osm-1(p808) X. Show Description
Osmotic non-avoider. M-MATING++ 1-10%WT.
PR816 C. elegans osm-1(p816) X. Show Description
Osmotic non-avoider.
BC13809 C. elegans dpy-5(e907) I; sIs13297. Show Description
sIs13297 [rCes T27B1.1::GFP + pCeh361]. Maintain by picking WT. WT animals are GFP+. Strain construction supported by Genome British Columbia and Genome Canada. Please acknowledge McKay et al, Cold Spring Harbor Symposia on Quantitative Biology 68: 159-169 2004 (WBPaper00006525).
SP797 C. elegans unc-3(e151) osm-1(p808) X; mnDp13 (X;f). Show Description
WT phenotype. Segregates Uncs which are Osm. [Osm phenotype checked 2/94.] mnDp3[sup-10(mn338)] and mnDp13 are the same. mnDp13 was derived from mnDp3 as lacking sup-10(+). It is not known if mnDp3 picked up a mutation in sup-10 or if the end of mnDp3 was shortened.
SP1882 C. elegans dpy-1(e1) ncl-1(e1865) III; unc-3(e151) osm-1(p808) X; mnDp92 [dpy-1(+) ncl-1(+) unc-3(+) osm-1(+)] (III;X;f). Show Description
Animals with mnDp92 are WT. Animals which have lost mnDp92 are DpyUncOsm and Ncl. Males containing mnDp92 are fertile. mnDp92 was derived by fusing mnDp14 and mnDp90.
SP1911 C. elegans dpy-1(e1) ncl-1(e1865) III; unc-3(e151) osm-1(p808) X; mnDp91 (III;X;f). Show Description
Animals with mnDp91 are WT. Animals which have lost mnDp91 are DpyUncOsm and Ncl. mnDp91 derived by fusing mnDp14 and mnDp90.
CG644 C. elegans pha-1(e2123) III; him-5(e1490) V; rgEx251. Show Description
rgEx251[pha-1(+) + osm-12 promoter:: unc-103(gf)]. Maintain at 20C.
HA1019 C. elegans osm-11(rt142) X. Show Description
Isolated from a deletion library.
HA2031 C. elegans rtIs31 X. Show Description
rtIs31 [elt-2p::GFP + Posm-10::HtnQ150].
HA3 C. elegans nuIs11. Show Description
nuIs11 [osm-10::GFP + lin-15(+)]. Array contains pool28; KP#57-59 (osm-10::GFP insert at Nrul site) and pJM24 [lin-15(+) rescues n765 at 20C]. GFP expressed in ASH, ASI, PHA and PHB after 3-fold. nuIs11 may be inserted on LG I.
HA659 C. elegans rtIs11 V. Show Description
rtIs11 [osm-10p::GFP + osm-10p::HtnQ150 + dpy-20(+)]. HtnQ150 causes ASH degeneration 30% at 8 days.
HA661 C. elegans rtIs18 I. Show Description
rtIs18 [elt-2p::GFP + osm-10p::HtnQ150].
HA759 C. elegans pqe-1(rt13) III; rtIs11 V. Show Description
rtIs11 [osm-10p::GFP + osm-10p::HtnQ150 + dpy-20(+)]. osm-10 promoter drives expression of both GFP and Htn-Q150 strongly in ASH and more weakly in other neurons of the head and tail. pqe-1(rt13) accelerates Htn-Q150 induced toxicity resulting in ASH neuron cell death predominantly during larval stages. Hence, many adult animals will lack overt GFP expression in ASH neurons. rtEx377 in the original HA759 was selected against leaving only rtIs11 in the strain available at the CGC.
KP696 C. elegans eos-2(nu268) III. Show Description
Weak Osm as homozygote. Non-allelic non-complementor of osm-10(n1602).
KP715 C. elegans eos-1(nu288) IV. Show Description
Weak Osm as homozygote. Non-allelic non-complementor of osm-10(n1602).
MT3641 C. elegans osm-10(n1602) III. Show Description
Osmotic avoidance defective. FITC fills most amphid cells.
MT3643 C. elegans osm-11(n1604) X. Show Description
MT3645 C. elegans osm-12(n1606) III. Show Description
Semidominant osmotic avoidance defective. FITC fills normally.
MT3664 C. elegans ric-1(n1600) III. Show Description
Osmotic avoidance defective. FITC fills normally. Previously called osm-13.
MT3743 C. elegans unc-84(e1410) osm-11(n1604) X. Show Description
OH4769 C. elegans ttx-3(mg158) X; nuIs11. Show Description
nuIs11 [osm-10::GFP + lin-15(+)]. Array contains pool28; KP#57-59 (osm-10::GFP insert at Nrul site) and pJM24 [lin-15(+) rescues n765 at 20C]. GFP expressed in ASH, ASI, PHA and PHB after 3-fold. nuIs11 may be inserted on LG I.
PS8846 C. elegans syIs707; syIs300. Show Description
syIs707 [osm-10p::NLS::cGAL(DBD)::gp41-1-N-intein::let-858 3'UTR, srb-6p::NLS::gp41-1-C-intein::cGAL(AD)::let-858 3'UTR + unc-122p::RFP + 1kb DNA ladder (NEB)] split cGAL driver for PHA and PHB neurons. syIs300 [15xUAS::(delta)pes-10::GFP::let-858 3'UTR + ttx-3p::RFP + 1kb DNA ladder(NEB)] is a GFP cGAL effector. Some worms do not express ttx-3p::RFP marker, but will consistently produce worms with the transgenic marker in next generation.
RB1268 C. elegans osm-12(ok1351) III. Show Description
Y75B8A.12 Homozygous. Outer Left Sequence: gcagaaaagaaaccaggcag. Outer Right Sequence: gcacccccacagtttttcta. Inner Left Sequence: ttccacgtcaccagatacca. Inner Right Sequence: ccccacagtgctcctacaat. Inner Primer PCR Length: 2849. Estimated Deletion Size: about 1300 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807
SRS85 C. elegans sraIs49 V; lite-1(ce314) X; sraEx80. Show Description
sraIs49 contains [nmr-1p::G-CaMP + unc-119(+)]. sraEx80 contains [sra-6p::chop-2(H134R)::mCherry + osm-10p::G-CaMP + unc-122p::mCherry]. Superficially wild-type. Maintain by picking red fluorescent worms.