Search Strains

Heterozygotes are WT and segregate WT, Unc-36 and dead eggs. Maintain by picking WT. Original isolation: BO strain BC1261 unc-22(s727) hermaphrodite heat shocked for 1 hour at 34C. Crossed to N2 strain BC1270 unc-22(s7) unc-31(e169)/nT1 IV; +/nT1 V. Picked individual WT hermaphrodites. Screened for the absence of gravid Unc-22. Retained strain as BC1379. Balanced over eT1: BC1379 unc-22(s727)[BO]/nT1[N2] IV; sDf31[BO]/nT1[N2] hermaphrodite crossed to BC 1265 dpy-18(e364)/eT1 III; unc-46(e177)/eT1 V. Pick WT hermaphrodites that twitch in 1% nicotine. Retained one strain that segregated Unc-36 as BC1428. Pseudolinked sDf31 to dpy-18: BC1428 +/eT1 III; sDf31[BO]/eT1 V crossed to BC 1265 dpy-18/eT1 III; unc-46/eT1 V male. Picked WT hermaphrodite F1. From strains segregating Unc-36 but no Dpy or Unc-46 or DpyUnc-46, cross WT hermaphrodites to BC1265 males. From strains producing Dpy, crossed individual WT males to BC70 eT1;eT1. From each cross, crossed WT X WT. Kept one strain producing on Dpy or DpyUncs as S-H716 male. Picked one hermaphrodite to start BC1519. Comments: 1) The LGV region balanced by eT1 should be all BO. 2) Probably the rest of the genome still has a considerable amount from BO since it was crossed to N2 only 4 times. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.

Animals with the duplication are WT. Animals which have lost the duplication are DpyUnc. Segregates 2% males. The males are sterile. The duplication is homozygous lethal.

Dpy. Best maintained at 16C (dpy-14 is ts). High recombination.

Heterozygotes are WT and segregate WT, Vul and UncLethals. Strong allele of let-60. Unc Twitchers arrest as rod-like early larvae; rare escapers die as Vul adults.

Heterozygotes are WT and segregate WT, Unc-36, DpyUncs which arrest as embryos or as early larva and dead eggs.

Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal early larval. Maintain by picking WT. [11/96: the strain is not throwing Unc-36 (eT1 homozygotes). eT1 may have picked up a lethal mutations which causes these animals to arrest, possibly as L1s.]

srl-2 is a recessive suppressor of the s1040 temperature sensitive lethal phenotype. srl-2 has no effect on the Roller phenotype of s1040. (At 20C, s1040 homozygotes arrest development at mid-larval stage. At 15C, s1040 homozygotes are viable weak left-handed rollers.) Maintain at 20C to select for retention of suppressor. Animals at 20C will be DpyUncRol.

Heterozygotes are WT and segregate WT, Vul, UncLet and dead eggs. Early larval arrest (L1-L2). Maintain by picking WT. See also WBPaper00002328 and WBPaper00003721.

Heterozygotes are WT and segregate WT, lethal Uncs (arrest as L1) and dead eggs. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.

Heterozygotes are WT (some seem to look a bit Dpyish) and segregate WT and dead eggs. sDf33 homozygotes arrest as embryos. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.

Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal embryo to early larval. Maintain by picking WT.

sDf83 homozygotes arrest as late larva or sterile adults after 2 weeks.

Heterozygotes are WT and segregate WT and dead eggs. sDf85 homozygotes arrest as embryos or early L1 larva. This strain was generated by the Genetic Toolkit project, which should be acknowledged in any publications resulting from its use: The Genetic Toolkit is funded by the NIH National Center for Research Resources (NCRR) (USA) to Ann M. Rose, David L. Baillie, and Donald L. Riddle. Report all experimental results to David Baillie.

srl-1 is a recessive suppressor of the s1040 temperature sensitive lethal phenotype. srl-1 has no effect on the Roller phenotype of s1040. (At 20C, s1040 homozygotes arrest development at mid-larval stage. At 15C, s1040 homozygotes are viable weak left-handed rollers.) Maintain at 20C to select for retention of suppressor. Animals at 20C will be DpyUncRol.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in mid to late larval development.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in early larval development. s2433 previously assigned to let-701.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and Sterile as adults.

Animals with the duplication are Unc. Animals which have lost the duplication arrest as embryos.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and give only dead eggs.

Maintain by picking Uncs. DpyUncs arrest as early larvae.

Maintain by picking Uncs. DpyUncs arrest at early larvae. s2441 previously assigned to let-703.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest as Sterile Adults.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest as early larva. s2443 pka let-708.

Maintain by picking Uncs. DpyUncs arrest as early larvae. This is a slow developing strain. Received new stock 9/03. 3/09: Buelow lab reported not seeing lethal DpyUncs.

Maintain by picking Uncs. DpyUncs arrest as early larvae. This is a slow developing strain.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUncs and are sterile adults.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUncs and arrest at the mid-larval stage of development.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest at a mid-larval stage of development.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUncs and are sterile adults (some lay eggs that don't hatch).

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest as Sterile Adults.

Maintain by picking Uncs. DpyUncs arrest at early to mid larval stage.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUncs which arrest at an early larval stage.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in mid-late larval development.

Maintain by picking Uncs. DpyUncs arrest at an early larval stage.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest at the early larval stage.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest as early larva.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and become sterile adults.

Maintain by picking Uncs. DpyUncs arrest as early larvae.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest in early larval development.

Maintain by picking Uncs. DpyUncs arrest at early to mid larval stage.

Maintain by picking Uncs. DpyUncs arrest as early larvae.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest at an early larval stage.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest at an early larval stage.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest as sterile adults.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest at an early larval stage, but s2572 is leaky.

Maintain by picking Uncs. DpyUncs arrest at early-mid larval stage.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest as Sterile Adults.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and are embyronic lethals.

Maintain by picking Uncs. DpyUncs arrest as early larvae. s2582 previously thought to be an allele of let-726.

Animals with the duplication are Unc. Animals which have lost the duplication are DpyUnc and arrest at the early larval stage.