Heterozygotes are WT and segregate WT, Unc-36, DpyUncs which arrest as embryos or as early larva and dead eggs.

Heterozygotes are WT and segregate WT, Unc-36, DpyUncLet and dead eggs. Lethal embryo to early larval. Maintain by picking WT.

Apparent homozygous lethal or sterile deletion as unbalanced heterozygote. Deletion of 9239 bp with Calarco/Colaiacovo selection cassette conferring myo-2 GFP and G418 resistance inserted at break. Pick viable fertile GFP+ animals to maintain. Left flanking sequence: GATACTCTCAAAATAATCGAGATTGCAGAA. Right flanking sequence: GTGGGAAAAACAAGGAGCAATTTCTATTTT. Please reference Au et al., G3 9(1): 135-144 2019 in any work resulting from use of this mutation.