Search Strains

aSi27 [lox2272::Cbr-unc-119(+)::lox2272::mec-7p::GCaMP7s::SL2::mScarlet-I] II. mec-7 promoter driving expression of GCaMP7s in ALM, PLM, AVM & PVM neurons. Reference: Ding J, et al. GE (Bethesda). 2023 Sep 30;13(10):jkad183. doi: 10.1093/g3journal/jkad183. PMID: 37565483.

aSi36 [lox2272::Cbr-unc-119(+)::lox2272::mec-7p::GCaMP7f::SL2::mScarlet-I] II. mec-7 promoter driving expression of GCaMP7f in ALM, PLM, AVM & PVM neurons. Reference: Ding J, et al. GE (Bethesda). 2023 Sep 30;13(10):jkad183. doi: 10.1093/g3journal/jkad183. PMID: 37565483.

isIs17 [pGZ295 (pie-1p::GFP::pcn-1(W03D2.4)) + pDP#MM051 (unc-119(+))] Maintain at 25C if possible to avoid possible germ line silencing. The entire coding sequence of PCNA (PCN-1, W03D2.4) was PCR-amplified and the resulting fragment cloned into pie-1::GFP germline expression vector to generate pGZ295, which was co-bombarded with pDP#MM051, which carries an unc-119 cDNA. The transgenic line behaves as though the two plasmids have been jointly integrated.

goeIs247 [ceh-24p::GCaMP6s::mKate2::unc-54 3'UTR + unc-119(+)]. Reporter expresses calcium sensor GCaMP6s with expression control mKate2. Reference: Schwarz J & Bringmann H. eLife 2017;10.7554/eLife.24846.

goeIs5 [nmr-1p::SL1::GCaMP3.35::SL2::unc-54 3'UTR + unc-119(+)]. Reporter allows visualization of several command interneurons. Reference: Schwarz J & Bringmann H. PLoS One. 2013 Sep 20;8(9):e75853.

goeIs22 [mec-4p::SL1::GCaMP3.35::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Reporter allows visualization of several mechanosensitive neurons. Reference: Schwarz J & Bringmann H. PLoS One. 2013 Sep 20;8(9):e75853.

goeIs3 [myo-3p::SL1::GCamP3.35::SL2::unc54 3'UTR + unc-119(+)]. Reporter expresses the calcium indicator GCaMP3 in all body wall muscles. Reference: Schwarz J, et al. Worm. 2012 Jan 1;1(1):12-4.

RNAi-capable OP50 strain. Tetracycline & Chloramphenicol resistant. Uracil auxotroph. E. coli B. Biosafety Level: BSL-1. Standard OP50 (CGC) was rendered RNAi competent through two modifications by phage transduction. The OP50 RNase III (rnc) allele was replaced with the deletion allele (rnc14::?Tn10) found in HT115(DE3), and a genomically encoded IPTG-inducible T7 RNA polymerase (lacz?A::T7pol camFRT) was introduced. Reference: Neve IAA, et al. G3 (Bethesda). 2019 Nov 11. pii: g3.400741.2019.

ufIs104 [nlp-12(+) + lgc-11p::GFP]. ufIs104 contains a 1.76 kb PCR product containing the nlp-12 genomic locus (?354 bp to +1407 bp relative to the transcriptional start). Over-expression of nlp-12 results in increased body bend amplitude. This strain has a high rate of sterility but can be successfully maintained as a homozygote. Reference: Bhattacharya R, et al. PLoS Genet. 2014 Aug 28;10(8):e1004584. doi: 10.1371/journal.pgen.1004584. PMID: 25167143.

axIs1460 [pie-1p::GFP::pal-1(genomic)::pal-1 3'UTR + unc-119(+)]. Transgene is prone to silencing -- maintain at 25C. pal-1 coding region and 3’ sequences were amplified from genomic DNA and cloned into pDONR201 to create ORF+3’ Entry Clones.

Low percent sterile, more sperm than WT, delayed oogenesis, larger broods than WT. PCR amplify: WT band is about 3 kb, deletion band is about 1.2 kb. Oligos used: outer: 201A and 202S; inner: 203A and 204S. Do not distribute this strain; other labs should request it from the CGC.

T05C12.6, T05C12.7. Heterozygotes are WT and GFP+ in the pharynx. mIn1[mIs14 dpy-10(e128)] homozygotes are Dpy and GFP+ in the pharynx. Homozygous mig-5&cct-1(ok280) worms arrest at about L2/L3. Strong loss of function for cct-1 and weak for mig-5. mig-5 is a dishelved homologue. External left primer: CGGCCTAAACGTTGATTGTT. External right primer: CAGAGTGAGTCGTGAACCGA. Internal left primer: TAATCCTGAATCCGGACGAG. Internal right primer: TACGAGATTTCGGTCCCTTG. Internal WT amplicon: 3284 bp. Deletion size: 987 bp. Deletion left flank: GCTGCAGTCTGATGTGCATGGCGGCTCCAT. Deletion right flank: TTGGTGTTGTCTATGCTTCAAGAAAATTGA. Do not distribute this strain; other labs should request it from the CGC.

B0273.2. External left primer: AAGCATACAGGCGCAGAGAT. External right primer: TACCGTATTGTGGTGCGAAA. Internal left primer: AACGGTTGCTCATCTGACTT. Internal right primer: AAAATTGCGTCGATTTTTGG. Internal WT amplicon: 2701 bp. Deletion size: 1751 bp. Deletion left flank: TTAAAACTTCTAATTTAAATAAAAAATATA. Deletion right flank: TATGTCGTTCAGCAAATGATTTCGATTTGC. Do not distribute this strain; other labs should request it from the CGC.

Recessive, loss of function, early stop mutation within the coding sequence makes sa603 a likely null allele. Variable locomotion ranging from lethargic to hyperactive. Eat (pale, scrawny). Loopy movement - increased amplitude of locomotory wave-form (variable). Suppresses the enteric muscle contraction (EMC) defect, the lethargy and egg-laying defects of unc-43(n498).

Recessive, early stop mutation within the coding sequence (C to T substitution in aa52) makes sa734 a likely null allele. May grow slightly better at 15C. Hyperactive, lays early stage eggs, increased amplitude of locomotort wave-form. Suppresses the lethargy and egg-laying defects of unc-43(n498). Reverses direction of locomotion more frequently than WT.

CRISPR-engineered KO strain of haps-1 made by knock-in of tandem stop codons to the first exon of endogenous haps-1 locus. haps-1(C48B6.3) is the C. elegans ortholog of human HAPSTR1. haps-1(ljt1) is superficially wild-type when cultured at 20C, but is hypersensitive to paraquat (redox stress), MLN4924 (neddylation inhibition), and camptothecin (genotoxic stress). Reference: Amici DR, et al. Proc Natl Acad Sci U S A. 2022 Jul 5;119(27):e2111262119. doi: 10.1073/pnas.2111262119. PMID: 35776542.

Isolated from rotting pears sampled below their tree on 15 Oct 2006 in Le Blanc (Indre, France). Sample plated on 16 Oct 2006. Picked as an adult on 18 Oct 2006. PrA1. For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Male-female strain. Isolated by Marie-Anne Felix from decomposing fruit and vegetables sampled on March 11, 2007, in small vegetable gardens in Okazaki (East of Nagoya), Aichi Prefecture, Japan. Isofemale line. Maintain by mating.

Male-female strain. Isolated by Marie-Anne Felix from decomposing fruit and vegetables sampled on March 14, 2007, in small vegetable gardens next to the Kacho-en aviary (ca. 100 m) in Kakegawa, Shizuoka prefecture, Japan. Isofemale line. Maintain by mating.

Isolated by Marie-Anne Felix from leaf litter/bark/soil sampled on March 14, 2007, in the woods behind the parking lot of the Kacho-en aviary in Kakegawa, Shizuoka prefecture, Japan (ca. 100 m from the aviary and 200 m from the vegetable gardens). For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Male-female strain. Isolated by Marie-Anne Felix from leaf litter/bark/soil sampled on March 14, 2007, in the woods behind the parking lot of the Kacho-en aviary in Kakegawa, Shizuoka prefecture, Japan (ca. 100 m from the aviary and 200 m from the vegetable gardens). Isofemale line. Maintain by mating.

Male-female strain. Isolated by Marie-Anne Felix from leaf litter/bark/soil sampled on March 18, 2007, in the Botanical Gardens of the University of Tokyo in Tokyo, Japan. Isofemale line. Maintain by mating.

Isolated by Marie-Anne Felix from soil sampled on March 14, 2007, in the Kacho-en aviary in Kakegawa, Shizuoka prefecture, Japan. For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Caenorhabditis elegans wild isolate. Isolated from a compost sample collected in April 2007 in Concepcion, Chile, in the Palomares area, 2 km NE of the town. For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Isolated from a sample of soil (compost) in a pot with rotting tomatoes collected in April 2007 in Concepcion, Chile, in the Villuco area, 3 km SE of the town. For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Caenorhabditis sp. 7 Male-female strain. Isolated by Marie-Anne Felix from rotting citrus fruit sampled by M. Herrmann in Begoro, Ghana in June 2007. New male-female species of the elegans group. Does not produce cross-progeny with any of the other previous elegans group species. Isofemale line. Maintain by mating.

Isolated by Tony Page from a compost heap sample recovered on 1 Aug 2007 in SouthWest Scotland 4°36.0' West 55°34.6' North. For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Caenorhabditis sp. 9 Male-female strain. Isolated from rotting flowers and leaves sampled in the Zoo/Botanical Garden of Trivandrum, Kerala, India on 21 Dec 2007. Culture at 20°C or above. Reference: Félix, Braendle & Cutter, 2014

Caenorhabditis sp. 10 Male-female strain. Isolated by Marie-Anne Félix from rotting cacao fruit sampled in Angela Spice Garden a few kilometers from Periyar, Kerala, India on 27 Dec 2007. Culture at 20°C or above.

Hermaphrodite. Isolated by MAF from a mix of rotting fruits, leaf litter, soil, bark, flowers, etc. sampled in a ca. 3 km wide area in Periyar Natural Preserve, Kerala, India on 28 Dec 2007. Isolated from a plate containing a rotting fruit similar to that of sample #46 - JU1361/2). For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Caenorhabditis sp. 11 Isolated by Marie-Anne Felix from rotting torch ginger (Etlingeria elatior) flowers sampled in the island of La Réunion by Valérie Robert and Loïc Sablé in Jan 2008. Hermaphrodite. Culture at 20°C or above. For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Isolated from rotting orange fruits, Garden Catalina de Ribera, Sevilla, Spain sampled on 29 Mar 08 by MAF. Not bleached. For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Isolated from Rumina decollata snail #4, sampled in front of the Parador of Carmona, Spain on 31 Mar 08 by MAF. Plated 3 Apr 08. Isolated as adult on 9 Apr 08. For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Caenorhabditis sp. 9 Male-female strain. Derived by 25 rounds of inbreeding (1 virgin female + 1 male) from JU1325, isolated from rotting flowers and leaves sampled in the Zoo/Botanical Garden of Trivandrum, Kerala, India on 21 Dec 2007. Culture at 20°C or above.

Caenorhabditis sp. 12 Male-female strain. Isolated in May 2008 from rotting Micropholis cayennensis fruit (#1, subsample L), sampled by P. Châtelet on the "Petit Plateau" near the CNRS Biological station, Nouragues, French Guyana.

Caenorhabditis sp. 11 Isolated by Marie-Anne Felix from rotting Duguetia surinamensis fruit, sampled by Patrick Châtelet on the "Petit Plateau" in the Nouragues Forest, French Guyana in May 2008. Hermaphrodite. Culture at 20°C or above. For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Isolated from rotting palm fruit sampled by MAF on 9 June 2008 in the Park Guëll, Barcelona, Spain. Picked as young adult on 11 June 2008. For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Caenorhabditis sp. 7 Male-female strain. Isolated from leaf litter near pond edge, sampled by J. and G. Hatty on 29 Nov 08 near Shonga, Nigeria.

Isolated by Rosina Giordano from compost sampled in Montevideo, Uruguay. For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Caenorhabditis sp. 17. Maintain at 23C. Isolated from rotten wide bean of Barokia sp. sampled on Grand Plateau in Nouragues Forest, French Guiana, on 21 Nov 2009. Reference: Félix, Braendle & Cutter, 2014

Isolated by M.-A. Félix from rotting fruits of Calophyllum inophyllum (red mahogany) sampled in the Jozani National Forest, Zanzibar, Tanzania (6.2715°S, 39.416°E), on 3/9/2012. Previously known as Caenorhabditis sp. 26.

Male-Female strain. Isolated by M.-A. Félix from a rotting mandarin sampled on a farm in the Dole district, Zanzibar, Tanzania (6.1074°S, 39.2515°E) , on 3/7/2012. Previously known as Caenorhabditis sp. 26.

Caenorhabditis elegans wild isolate. From Le Blanc (Indre, France). From a vegetable garden garbage pile. Same soil sample as JU262. Old adults in this strain get vacuoles in their intestinal cells. For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Male-female strain. Isolated from a rotting fruit sampled on 09 June 2014 by Fabrice Besnard in Guadeloupe (Basse Terre, 16.1761, -61.6951). Started with a plugged female. Previously known as Caenorhabditis sp. 38.

Isolated from humus sampled on 08 August 2014 below the cathedral fig tree Ficus destruens by Danbulla Road, Australia (-17.1774, 145.6600) by Jean-Baptiste Pénigault. Started on 19 August from a plugged female. Crossed with sp. 5 JU727 in both directions; the outcome is more than 100 embryos, no larvae. Previously known as Caenorhabditis sp. 40.

Isolated on Sept 8, 2002, from garden soil in Merlet, Lagorce (Ardeche), France. See Barriere & Felix, Current Biology 2005 (sample Merlet 1). For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Isolated on Sept 8, 2002, from a Helix snail on the trunk of a mulberry tree in Merlet, Lagorce (Ardeche), France. See Barriere & Felix, Current Biology 2005 (sample Merlet 2). For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Male-female species. Maintain at 20C or warmer. Elegans group. Isolated from rotting stems of banana family (Musaceae) sampled on 5 March 2018 in Pugao Laozhai village, Yuanyang, Yunnan, China. 23.0854, 102.7993. Reference: Felix et al., in preparation.

Isolated on Sept 8, 2002, from a Glomeris myriapod in a compost pile in Merlet, Lagorce (Ardeche), France. See Barriere & Felix, Current Biology 2005 (sample Merlet 3). For whole-genome sequence-verified wild strains, please request from the Caenorhabditis Natural Diversity Resource (www.caendr.org).

Male-female species. Maintain at 20C or warmer. Elegans group. Isolated from rotting flower on upright Musa coccinea plant sampled near Sapa, Vietnam on 30 Nov 2019. GPS 22.33947, 103.86148. Reference: Felix et al., in preparation.