T05C12.6, T05C12.7. Heterozygotes are WT and GFP+ in the pharynx. mIn1[mIs14 dpy-10(e128)] homozygotes are Dpy and GFP+ in the pharynx. Homozygous mig-5&cct-1(ok280) worms arrest at about L2/L3. Strong loss of function for cct-1 and weak for mig-5. mig-5 is a dishelved homologue. External left primer: CGGCCTAAACGTTGATTGTT. External right primer: CAGAGTGAGTCGTGAACCGA. Internal left primer: TAATCCTGAATCCGGACGAG. Internal right primer: TACGAGATTTCGGTCCCTTG. Internal WT amplicon: 3284 bp. Deletion size: 987 bp. Deletion left flank: GCTGCAGTCTGATGTGCATGGCGGCTCCAT. Deletion right flank: TTGGTGTTGTCTATGCTTCAAGAAAATTGA. Do not distribute this strain; other labs should request it from the CGC. This strain cannot be distributed to commercial organizations. This strain cannot be used for any commercial purpose or for work on human subjects.

K02G10.7. Homozygous. Outer Left Sequence: AAGCAGAAAAGGAGCGTGAA. Outer Right Sequence: TCAGGGCCTACCGTAACATC. Inner Left Sequence: ATGTTTTAGAGGGCGGTTGC. Inner Right Sequence: GCCCATTCTGAAGTTTCGAC. Inner Primer PCR Length: 1177 bp. Deletion Size: 436 bp. Deletion left flank: TAACTATGTATAAACATGGATCAGAAGTTG. Deletion right flank: ATTCCCCAGGCAATATGGCAAGACGTTGAG. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

M01H9.3 Homozygous. Outer Left Sequence: ttgtttgtttgacggaacca. Outer Right Sequence: tgggtcccaattcaaatcat. Inner Left Sequence: tgggaaaagcgtgagctact. Inner Right Sequence: gctgaatacttctcaccgcc. Inner Primer PCR Length: 1207. Deletion size: about 900 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

D1086.6 Homozygous. Outer Left Sequence: cgatgatcaaaaacgctgtg. Outer Right Sequence: gagaagcagcacgtgttcag. Inner Left Sequence: cgacaagacaaaagaagttatttaca. Inner Right Sequence: gactgatcacagtgcggaaa. Inner Primer PCR Length: 1203. Deletion size: about 400 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F59B1.9 Homozygous. Outer Left Sequence: acatgtagacgtcgatggca. Outer Right Sequence: cagaatgagccgcaacaata. Inner Left Sequence: gtttccaaacgcacacattg. Inner Right Sequence: cgtgggcggtagaggtaaat. Inner Primer PCR Length: 1100. Deletion size: about 700 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

Y6B3B.5 Homozygous. Outer Left Sequence: aaattccttgatttggtgcg. Outer Right Sequence: cattccacacgacgaaaatg. Inner Left Sequence: tccgcgtccaatacaataca. Inner Right Sequence: aatgcaggtcaaactccacc. Inner Primer PCR Length: 1131. Deletion size: about 700 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

F35C5.7 Homozygous. Outer Left Sequence: ccggaaatttttattccggt. Outer Right Sequence: aatttttgtttgtgccccac. Inner Left Sequence: gcaggaaattttcattccga. Inner Right Sequence: ggctccaaaacagcaaagtc. Inner Primer PCR Length: 1104. Deletion size: about 600 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

C56E6.3 Homozygous. Outer Left Sequence: ttttgtcgcagttcaacgag. Outer Right Sequence: gcgacaatatcggaagaagc. Inner Left Sequence: gccaaaatctctgtttttcagg. Inner Right Sequence: caggcttggaggagattctg. Inner Primer PCR Length: 1177. Deletion size: about 600 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

Y39A3CL.5 Homozygous. Outer Left Sequence: cttttggtttttgcggagtc. Outer Right Sequence: caaggcgggacttgattaaa. Inner Left Sequence: cagggatcactggagccac. Inner Right Sequence: tcgaaaaatttcgggaaaaa. Inner Primer PCR Length: 1206. Deletion size: about 600 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

ZC487.5 Homozygous. Outer Left Sequence: caagaagccaactcctcgtc. Outer Right Sequence: acttcgcggttttcttctga. Inner Left Sequence: accccatccttaaaaccgac. Inner Right Sequence: cctcagccatcattccactt. Inner Primer PCR Length: 1292. Deletion size: about 500 bp. Attribution: This strain was provided by the C. elegans Gene Knockout Project at the Oklahoma Medical Research Foundation, which was part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807

ZC376.3. Homozygous lethal deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP ok2803 homozygotes (early larval arrest). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: ATAAGGCCCGAATAGCTTGG. External right primer: AGACCAAAACGGCAATTCAT. Internal left primer: TTTTTCATTAGAACACAAACAACAC. Internal right primer: CGAGATTGACAACAAGTGTGC. Internal WT amplicon: 3073 bp. Deletion size: 1600 bp. Deletion left flank: AAAAAATCCGTTACATTCACGAAAATTGAA. Deletion right flank: TTTGCATACAAACAATCTTCAGAAATCGGT. Insertion Sequence: TT. Attribution: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence WBPaper00041807