Strain Information
| Name | GZ264 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | unc-119(ed3) III; isIs17. |
| Description | isIs17 [pGZ295 (pie-1p::GFP::pcn-1(W03D2.4)) + pDP#MM051 (unc-119(+))] Maintain at 25C if possible to avoid possible germ line silencing. The entire coding sequence of PCNA (PCN-1, W03D2.4) was PCR-amplified and the resulting fragment cloned into pie-1::GFP germline expression vector to generate pGZ295, which was co-bombarded with pDP#MM051, which carries an unc-119 cDNA. The transgenic line behaves as though the two plasmids have been jointly integrated. |
| Mutagen | |
| Made by | Michael Brauchle |
| Laboratory | GZ |
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