Strain Information

Name iOP50   View On Wormbase
Species E. coli
GenotypeOP50 E. coli, rnc14::(delta)Tn10, lacz(gamma)A::T7pol camFRT
DescriptionRNAi-capable OP50 strain. Tetracycline & Chloramphenicol resistant. Uracil auxotroph. E. coli B. Biosafety Level: BSL-1. Standard OP50 (CGC) was rendered RNAi competent through two modifications by phage transduction. The OP50 RNase III (rnc) allele was replaced with the deletion allele (rnc14::ΔTn10) found in HT115(DE3), and a genomically encoded IPTG-inducible T7 RNA polymerase (laczγA::T7pol camFRT) was introduced. Reference: Neve IAA, et al. G3 (Bethesda). 2019 Nov 11. pii: g3.400741.2019.
MutagenPhage transduction (2x)
Made byJessica Sowa, Isaiah Neve
Laboratory MCW
Reference Neve IAA, Sowa JN, Lin CJ, Sivaramakrishnan P, Herman C, Ye Y, Han L, Wang MC. Escherichia coli Metabolite Profiling Leads to the Development of an RNA Interference Strain for Caenorhabditis elegans. G3 (Bethesda). 2019 Nov 11 pii: g3.400741.2019. doi: 10.1534/g3.119.400741. PMID: 31712257
Sign in or register an account if you want to order this strain.