Strain Information
| Name | iOP50 View On Wormbase |
|---|---|
| Species | E. coli |
| Genotype | OP50 E. coli, rnc14::(delta)Tn10, lacz(gamma)A::T7pol camFRT |
| Description | RNAi-capable OP50 strain. Tetracycline & Chloramphenicol resistant. Uracil auxotroph. E. coli B. Biosafety Level: BSL-1. Standard OP50 (CGC) was rendered RNAi competent through two modifications by phage transduction. The OP50 RNase III (rnc) allele was replaced with the deletion allele (rnc14::ΔTn10) found in HT115(DE3), and a genomically encoded IPTG-inducible T7 RNA polymerase (laczγA::T7pol camFRT) was introduced. Reference: Neve IAA, et al. G3 (Bethesda). 2019 Nov 11. pii: g3.400741.2019. |
| Mutagen | Phage transduction (2x) |
| Outcrossed | x0 |
| Made by | Jessica Sowa, Isaiah Neve |
| Laboratory | MCW |
| Reference | Neve IAA, Sowa JN, Lin CJ, Sivaramakrishnan P, Herman C, Ye Y, Han L, Wang MC. Escherichia coli Metabolite Profiling Leads to the Development of an RNA Interference Strain for Caenorhabditis elegans. G3 (Bethesda). 2019 Nov 11 pii: g3.400741.2019. doi: 10.1534/g3.119.400741. PMID: 31712257 |
Sign in
or
register an account if you want to order this strain.