| JK654 |
C. elegans |
fem-3(q23) IV. Show Description
Maintain at 15C. Do not distribute this strain; other labs should request it from the CGC.
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| MQD1543 |
C. elegans |
daf-16(hq23[daf-16::GFP]) I. Show Description
GFP tag inserted at the 3' end of the endogenous daf-16 gene locus by CRISPR/Cas9 engineering. The GFP fusion tag does not interfere with the function of DAF-16 protein. DAF-16::GFP is expressed ubiquitously in most or all somatic tissues, including neurons, intestine, body wall muscles, and hypodermis, and also in the germ cells and oocytes. Reference: Zhang Y, et al. BioRxiv. 2021 Aug 2. 2007.2031.454567. https://doi.org/10.1101/2021.07.31.454567.
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| EL69 |
C. elegans |
unc-32(e189) glp-1(q231) III; sog-3(q294) IV. Show Description
Unc. Fertile with viable progeny at or below 20C. Glp-1 sterile at higher temperatures. No obvious visible phenotype associated with sog-3.
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| JK1195 |
C. elegans |
lin-12(q269) glp-1(q231)/unc-32(e189) III. Show Description
Heterozygotes are WT and segregate WT, Uncs, and Lags (ts). Do not distribute this strain; other labs should request it from the CGC.
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| JK509 |
C. elegans |
glp-1(q231) III. Show Description
Temperature sensitive. Sterile at 25C. Fertile at 15C. Do not distribute this strain; other labs should request it from the CGC.
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| JK892 |
C. elegans |
unc-32(e189) glp-1(q231)/eT1 III; +/eT1 V. Show Description
Balanced temperature-sensitive allele of glp-1. Maintain at 20-25C. At restrictive temperature (25C), heterozygotes are wild-type and segregate wild-type, Unc-36 eT1 homozygotes, UncSteriles, and arrested eT1 aneuploid progeny (dead eggs). At permissive temperature (15C), heterozygotes are wild-type and segregate wild-type, Unc-36 eT1 homozygotes, fertile Unc-32 (can be maintained as a homozygous stock at 15C), and arrested eT1 aneuploid progeny (dead eggs). Maintain by picking wild-type and check for correct segregation of progeny to maintain. eT1 previously known as unc-36(e873). Do not distribute this strain; other labs should request it from the CGC.
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| JK907 |
C. elegans |
mog-4(q233)/mnC1 [dpy-10(e128) unc-52(e444)] II. Show Description
Do not distribute this strain; other labs should request it from the CGC.
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| JK948 |
C. elegans |
unc-32(e189) glp-1(q231) III; sog-4(q304) V. Show Description
Unc. sog-4 suppresses glp-1 at or below 20C. Do not maintain above 20C. Some Glp dead embryos because suppression is incomplete. Do not distribute this strain; other labs should request it from the CGC.
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| JK967 |
C. elegans |
ubr-5(q298) I; unc-32(e189) glp-1(q231) III. Show Description
Unc. Fertile with viable progeny at or below 20C. Glp-1 sterile at higher temperatures. No obvious visible phenotype associated with sog-1. Do not distribute this strain; other labs should request it from the CGC.
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| JK968 |
C. elegans |
unc-32(e189) glp-1(q231) III; sog-6(q306) IV. Show Description
Unc. Fertile with viable progeny at or below 20C. Glp-1 sterile at higher temperatures. No obvious visible phenotype associated with sog-6. Do not distribute this strain; other labs should request it from the CGC.
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| MT4925 |
C. elegans |
glp-1(q231) ced-7(n1892) unc-69(e587) III. Show Description
Maintain at 15C. glp-1(q231) is temperature sensitive. Unc. ced-7(n1892) causes persistent cell corpes and has a maternal effect.
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| TQ233 |
C. elegans |
trpa-1(ok999) IV. Show Description
Shorter lifespan than wild-type worms at 15-20 C, but not at 25 C. Reference: Xiao R, et al. Cell. 2013 Feb 14;152(4):806-17.
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