Strain Information
| Name | WRM104 View On Wormbase |
|---|---|
| Species | C. elegans |
| Genotype | pgl-1(spr20[mCherry::pgl-1]) IV; pos-1(spr28[(gfp::tev::3xFlag::pos-1::(delta)3' UTR *tn1730]) V. |
| Description | mCherry tag inserted at N-terminus of endogenous pgl-1 locus. mCherry fluorescence in P-granules throughout embryogenesis and larval morphogenesis. spr28 is a 239-nucleotide deletion (bp 45-285) and insertion of a jump board gRNA sequence (Duan et al. 2020) within the 3' UTR of the endogenously-tagged pos-1 locus. GFP::POS-1 protein expression is mis-regulated causing overexpression throughout the gonad where it is not normally expressed. A large portion of progeny fail to properly develop a germline. Other phenotypes include reduced brood size, reduced hatch rate, hermaphrodites developing only one gonad arm, oogenesis defects, and multi-vulva. Temperature stress of 25C increases severity of phenotypes. Derived by crossing WRM101 males with WRM85 hermaphrodites. Reference: Varderesian HV, et al. PLoS Genet. 2026 Apr 27;22(4):e1012129. doi: 10.1371/journal.pgen.1012129. PMID: 42044121. |
| Mutagen | N/A |
| Outcrossed | x0 |
| Made by | Haik V. Varderesian |
| Laboratory | WRM |
| Reference | Varderesian HV, et al. PLoS Genet. 2026 Apr 27;22(4):e1012129. doi: 10.1371/journal.pgen.1012129. PMID: 42044121. |
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