Laboratory Information
| Name | WRM View on WormBase |
|---|---|
| Allele designation | spr |
| Head | Sean Patrick Ryder |
| Institution | University of Massachusetts Medical School, Worcester, MA |
| Address | University of Massachusetts Medical School LRB-906 364 Plantation Street Worcester 01605 United States |
| Website | http://www.umassmed.edu/bmp/faculty/ryder.cfm |
| Gene classes |
Strains contributed by this laboratory
| Strain | Genotype | Species | Description |
|---|---|---|---|
| WRM1 | sprSi1 II; unc-119(ed3) III. | C. elegans | sprSi1 [pie-1p::GFP::histone-H2B::nos-2(MRE mut) 3'UTR + Cbr-unc-119(+)] II. Nuclear GFP fluorescence in germline progenitor cells in early embryos. Reference: Pagano JM, et al. Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20252-7. |
| WRM10 | sprSi10 II; unc-119(ed3) III. | C. elegans | sprSi10 [mex-5p::MODC PEST::GFP::H2B::atg-4.2 3'UTR + Cbr-unc-119(+)] II. Reference: Kaymak et al., Dev Dyn. 2016 Sep;245(9):925-36. |
| WRM12 | sprSi11 II; unc-119(ed3) III. | C. elegans | sprSi11 [mex-5p::MODC PEST::GFP::H2B::cul-4 3'UTR + Cbr-unc-119(+)] II. Reference: Kaymak et al., Dev Dyn. 2016 Sep;245(9):925-36. |
| WRM17 | sprSi13 II; unc-119(ed3) III. | C. elegans | sprSi13 [mex-5p::MODC PEST::GFP::H2B::ets-4 3'UTR + Cbr-unc-119(+)] II. Reference: Kaymak et al., Dev Dyn. 2016 Sep;245(9):925-36. |
| WRM18 | sprSi14 II; unc-119(ed3) III. | C. elegans | sprSi14 [mex-5p::MODC PEST::GFP::H2B::hbl-1 3'UTR + Cbr-unc-119(+)] II. Reference: Kaymak et al., Dev Dyn. 2016 Sep;245(9):925-36. |
| WRM19 | sprSi15 II; unc-119(ed3) III. | C. elegans | sprSi15 [mex-5p::MODC PEST::GFP::H2B::lin-26 3'UTR + Cbr-unc-119(+)] II. Reference: Kaymak et al., Dev Dyn. 2016 Sep;245(9):925-36. |
| WRM2 | sprSi2 II; unc-119(ed3) III. | C. elegans | sprSi2 [pie-1p::GFP::histone-H2B::nos-2 3'UTR + Cbr-unc-119(+)] II. Fluorescence in all cells of early embryo. This fluorescence reporter has mutations in both MEX-3 binding sites and shows ectopic expression relative to strain WRM1. Reference: Pagano JM, et al. Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20252-7. |
| WRM22 | sprSi16 II; unc-119(ed3) III. | C. elegans | sprSi16 [mex-5p::MODC PEST::GFP::H2B::mbk-2 3'UTR + Cbr-unc-119(+)] II. Reference: Kaymak et al., Dev Dyn. 2016 Sep;245(9):925-36. |
| WRM24 | sprSi17 II; unc-119(ed3) III. | C. elegans | sprSi17 [mex-5p::MODC PEST::GFP::H2B::mex-3 3'UTR + Cbr-unc-119(+)] II. Reference: Kaymak et al., Dev Dyn. 2016 Sep;245(9):925-36. |
| WRM27 | sprSi19 II; unc-119(ed3) III. | C. elegans | sprSi19 [mex-5p::MODC PEST::GFP::H2B::set-6 3'UTR + Cbr-unc-119(+)] II. Reference: Kaymak et al., Dev Dyn. 2016 Sep;245(9):925-36. |
| WRM3 | sprSi3 II; unc-119(ed3) III. | C. elegans | sprSi3 [mex-5p::GFP::histone-H2B::glp-1 3'UTR + Cbr-unc-119(+)] II. Fluorescence in the distal germline and in early embryos. Reference: Pagano JM, et al. Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20252-7. |
| WRM30 | sprSi20 II; unc-119(ed3) III. | C. elegans | sprSi20 [mex-5p::MODC PEST::GFP::H2B::usp-14 3'UTR + Cbr-unc-119(+)] II. Reference: Kaymak et al., Dev Dyn. 2016 Sep;245(9):925-36. |
| WRM31 | sprDf1 V/nT1 [qIs51] (IV,V). | C elegans | Pick GFP+ to maintain. sprDf1 is a ~0.25 Mb microdeletion allele on the left arm of chromosome V that removes 32 adjacent protein-coding genes, including mex-5. Heterozygous animals (GFP+) are fertile, but sometimes die by bursting, will have polynucleated embryos, and form uterine tumors ~6 days after hatching. sprDf1 homozygotes (GFP-) have maternal effect lethality, are small, sterile, form large uterine tumors that consist of poly nucleated embryos, have squashed vulvas, are uncoordinated, and die by bursting within eight days of hatching. nT1 homozygotes are inviable (dead eggs). Reference: Antkowiak KR, et al. G3 (Bethesda). 2023 Nov 13:jkad258. doi: 10.1093/g3journal/jkad258. PMID: 37956108. |
| WRM45 | mex-3(spr5[*tn1753]) I. | C. elegans | Homozygous fertile; reduced brood size. mex-3(spr5) is a CRISPR/Cas9 engineered deletion removing 488 bp of the mex-3 3´UTR. Derived by modification of parental strain DG4269 mex-3(tn1753[gfp::3xflag::mex-3]) I. Reference: Albarqi MMY & Ryder SP. PLoS Genet 2021 Aug 23;17(8):e1009775. PMID: 34424904 |
| WRM49 | mex-3(spr6[*tn1753]) I. | C. elegans | Homozygous fertile. mex-3(spr6) is a CRISPR/Cas9 engineered deletion removing 142 bp of the mex-3 3´UTR. Derived by modification of parental strain DG4269 mex-3(tn1753[gfp::3xflag::mex-3]) I. Reference: Albarqi MMY & Ryder SP. PLoS Genet 2021 Aug 23;17(8):e1009775. PMID: 34424904 |
| WRM5 | sprSi5 II; unc-119(ed3) III. | C. elegans | sprSi5 [mex-5p::MODC PEST::GFP::H2B::glp-1 3'UTR + Cbr-unc-119(+)] II. Reference: Farley BM, Ryder SP. Mol Biol Cell. 2012 Oct 3. |
| WRM50 | mex-3(spr7[*tn1753]) I. | C. elegans | Homozygous fertile. mex-3(spr7) is a CRISPR/Cas9 engineered deletion removing 134 bp of the mex-3 3´UTR. Derived by modification of parental strain DG4269 mex-3(tn1753[gfp::3xflag::mex-3]) I. Reference: Albarqi MMY & Ryder SP. PLoS Genet 2021 Aug 23;17(8):e1009775. PMID: 34424904 |
| WRM52 | mex-3(spr9[*tn1753]) I. | C. elegans | Homozygous fertile; reduced brood size. mex-3(spr9) is a CRISPR/Cas9 engineered deletion of the mex-3 3´UTR (removes 624 of 689 bp). Derived by modification of parental strain DG4269 mex-3(tn1753[gfp::3xflag::mex-3]) I. Reference: Albarqi MMY & Ryder SP. PLoS Genet 2021 Aug 23;17(8):e1009775. PMID: 34424904 |
| WRM53 | mex-3(spr10[*tn1753]) I. | C. elegans | Homozygous fertile; reduced brood size. mex-3(spr5) is a CRISPR/Cas9 engineered deletion removing 190 bp of the mex-3 3´UTR. Derived by modification of parental strain DG4269 mex-3(tn1753[gfp::3xflag::mex-3]) I. Reference: Albarqi MMY & Ryder SP. PLoS Genet 2021 Aug 23;17(8):e1009775. PMID: 34424904 |
| WRM6 | sprSi6 II; unc-119(ed3) III. | C. elegans | sprSi6 [mex-5p::MODC PEST::GFP::H2B::glp-1 ( |
| WRM7 | sprSi7II; unc-119(ed3) III. | C. elegans | sprSi7 [mex-5p::MODC PEST::GFP::H2B::glp-1 ( |
| WRM8 | sprSi8II; unc-119(ed3) III. | C. elegans | sprSi8 [mex-5p::MODC PEST::GFP::H2B::glp-1 ( |
| WRM9 | sprSi9II; unc-119(ed3) III. | C. elegans | sprS9 [mex-5p::MODC PEST::GFP::H2B::glp-1 ( |
This laboratory hasn't submitted any alleles to the CGC.