Laboratory Information
Name | WRM View on WormBase |
---|---|
Allele designation | spr |
Head | Sean Patrick Ryder |
Institution | University of Massachusetts Medical School, Worcester, MA |
Address | University of Massachusetts Medical School LRB-906 364 Plantation Street Worcester 01605 United States |
Website | http://www.umassmed.edu/bmp/faculty/ryder.cfm |
Gene classes |
Strains contributed by this laboratory
Strain | Genotype | Species | Description |
---|---|---|---|
WRM1 | sprSi1 II; unc-119(ed3) III. | C. elegans | sprSi1 [pie-1p::GFP::histone-H2B::nos-2(MRE mut) 3'UTR + Cbr-unc-119(+)] II. Nuclear GFP fluorescence in germline progenitor cells in early embryos. Reference: Pagano JM, et al. Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20252-7. |
WRM10 | sprSi10 II; unc-119(ed3) III. | C. elegans | sprSi10 [mex-5p::MODC PEST::GFP::H2B::atg-4.2 3'UTR + Cbr-unc-119(+)] II. Reference: Kaymak et al., Dev Dyn. 2016 Sep;245(9):925-36. |
WRM12 | sprSi11 II; unc-119(ed3) III. | C. elegans | sprSi11 [mex-5p::MODC PEST::GFP::H2B::cul-4 3'UTR + Cbr-unc-119(+)] II. Reference: Kaymak et al., Dev Dyn. 2016 Sep;245(9):925-36. |
WRM17 | sprSi13 II; unc-119(ed3) III. | C. elegans | sprSi13 [mex-5p::MODC PEST::GFP::H2B::ets-4 3'UTR + Cbr-unc-119(+)] II. Reference: Kaymak et al., Dev Dyn. 2016 Sep;245(9):925-36. |
WRM18 | sprSi14 II; unc-119(ed3) III. | C. elegans | sprSi14 [mex-5p::MODC PEST::GFP::H2B::hbl-1 3'UTR + Cbr-unc-119(+)] II. Reference: Kaymak et al., Dev Dyn. 2016 Sep;245(9):925-36. |
WRM19 | sprSi15 II; unc-119(ed3) III. | C. elegans | sprSi15 [mex-5p::MODC PEST::GFP::H2B::lin-26 3'UTR + Cbr-unc-119(+)] II. Reference: Kaymak et al., Dev Dyn. 2016 Sep;245(9):925-36. |
WRM2 | sprSi2 II; unc-119(ed3) III. | C. elegans | sprSi2 [pie-1p::GFP::histone-H2B::nos-2 3'UTR + Cbr-unc-119(+)] II. Fluorescence in all cells of early embryo. This fluorescence reporter has mutations in both MEX-3 binding sites and shows ectopic expression relative to strain WRM1. Reference: Pagano JM, et al. Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20252-7. |
WRM22 | sprSi16 II; unc-119(ed3) III. | C. elegans | sprSi16 [mex-5p::MODC PEST::GFP::H2B::mbk-2 3'UTR + Cbr-unc-119(+)] II. Reference: Kaymak et al., Dev Dyn. 2016 Sep;245(9):925-36. |
WRM24 | sprSi17 II; unc-119(ed3) III. | C. elegans | sprSi17 [mex-5p::MODC PEST::GFP::H2B::mex-3 3'UTR + Cbr-unc-119(+)] II. Reference: Kaymak et al., Dev Dyn. 2016 Sep;245(9):925-36. |
WRM27 | sprSi19 II; unc-119(ed3) III. | C. elegans | sprSi19 [mex-5p::MODC PEST::GFP::H2B::set-6 3'UTR + Cbr-unc-119(+)] II. Reference: Kaymak et al., Dev Dyn. 2016 Sep;245(9):925-36. |
WRM3 | sprSi3 II; unc-119(ed3) III. | C. elegans | sprSi3 [mex-5p::GFP::histone-H2B::glp-1 3'UTR + Cbr-unc-119(+)] II. Fluorescence in the distal germline and in early embryos. Reference: Pagano JM, et al. Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20252-7. |
WRM30 | sprSi20 II; unc-119(ed3) III. | C. elegans | sprSi20 [mex-5p::MODC PEST::GFP::H2B::usp-14 3'UTR + Cbr-unc-119(+)] II. Reference: Kaymak et al., Dev Dyn. 2016 Sep;245(9):925-36. |
WRM45 | mex-3(spr5[*tn1753]) I. | C. elegans | Homozygous fertile; reduced brood size. mex-3(spr5) is a CRISPR/Cas9 engineered deletion removing 488 bp of the mex-3 3´UTR. Reference: Albarqi MMY & Ryder SP. PLoS Genet 2021 Aug 23;17(8):e1009775. PMID: 34424904 |
WRM49 | mex-3(spr6[*tn1753]) I. | C. elegans | Homozygous fertile. mex-3(spr6) is a CRISPR/Cas9 engineered deletion removing 142 bp of the mex-3 3´UTR. Reference: Albarqi MMY & Ryder SP. PLoS Genet 2021 Aug 23;17(8):e1009775. PMID: 34424904 |
WRM5 | sprSi5 II; unc-119(ed3) III. | C. elegans | sprSi5 [mex-5p::MODC PEST::GFP::H2B::glp-1 3'UTR + Cbr-unc-119(+)] II. Reference: Farley BM, Ryder SP. Mol Biol Cell. 2012 Oct 3. |
WRM50 | mex-3(spr7[*tn1753]) I. | C. elegans | Homozygous fertile. mex-3(spr7) is a CRISPR/Cas9 engineered deletion removing 134 bp of the mex-3 3´UTR. Reference: Albarqi MMY & Ryder SP. PLoS Genet 2021 Aug 23;17(8):e1009775. PMID: 34424904 |
WRM52 | mex-3(spr9[*tn1753]) I. | C. elegans | Homozygous fertile; reduced brood size. mex-3(spr9) is a CRISPR/Cas9 engineered deletion of the mex-3 3´UTR (removes 624 of 689 bp). Reference: Albarqi MMY & Ryder SP. PLoS Genet 2021 Aug 23;17(8):e1009775. PMID: 34424904 |
WRM53 | mex-3(spr10[*tn1753]) I. | C. elegans | Homozygous fertile; reduced brood size. mex-3(spr5) is a CRISPR/Cas9 engineered deletion removing 190 bp of the mex-3 3´UTR. Reference: Albarqi MMY & Ryder SP. PLoS Genet 2021 Aug 23;17(8):e1009775. PMID: 34424904 |
WRM6 | sprSi6 II; unc-119(ed3) III. | C. elegans | sprSi6 [mex-5p::MODC PEST::GFP::H2B::glp-1 ( |
WRM7 | sprSi7II; unc-119(ed3) III. | C. elegans | sprSi7 [mex-5p::MODC PEST::GFP::H2B::glp-1 ( |
WRM8 | sprSi8II; unc-119(ed3) III. | C. elegans | sprSi8 [mex-5p::MODC PEST::GFP::H2B::glp-1 ( |
WRM9 | sprSi9II; unc-119(ed3) III. | C. elegans | sprS9 [mex-5p::MODC PEST::GFP::H2B::glp-1 ( |
This laboratory hasn't submitted any alleles to the CGC.